LOCUS LT727351 8946 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pBATEM2, complete sequence.
ACCESSION LT727351
VERSION LT727351.1
KEYWORDS .
SOURCE Mammalian expression vector pBATEM2
ORGANISM Mammalian expression vector pBATEM2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8946)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..8946
/organism="Mammalian expression vector pBATEM2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 3585."
/db_xref="taxon:1944750"
CDS <1..809
/codon_start=3
/transl_table=11
/note="part of SV40 VP1"
/protein_id="SJL88185.1"
/translation="PLPNLNEDLTCGNILMWEAVTVKTEVIGVTAMLNLHSGTQKTHE
NGAGKPIQGSNFHFFAVGGEPLELQGVLANYRTKYPAQTVTPKNATVDSQQMNTDHKA
VLDKDNAYPVECWVPDPSKNENTRYFGTYTGGENVPPVLHITNTATTVLLDEQGVGPL
CKADSLYVSAVDICGLFTNTSGTQQWKGLPRYFKITLRKRSVKNPYPISFLLSDLINR
RTQRVDGQPMIGMSSQVEEVRVYEDTEELPGDPDMIRYIDEFGQTTTRMQ"
regulatory complement(810..906)
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory 810..906
/note="SV40 polyA late"
/regulatory_class="terminator"
misc_feature complement(907..1458)
/note="3' UTR SV40 small t-antigen"
misc_feature complement(1459..1525)
/note="intron of SV40 small t-antigen"
CDS complement(1526..>1600)
/codon_start=1
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL88186.1"
/translation="DLCEGTLLLWCDIIGQTTYRDLKL"
misc_feature complement(1876..2265)
/note="3' UTR of mCdh1"
CDS complement(2266..4920)
/transl_table=11
/note="mCdh1"
/protein_id="SJL88187.1"
/translation="MGARCRSFSALLLLLQVSSWLCQELEPESCSPGFSSEVYTFPVP
ERHLERGHVLGRVRFEGCTGRPRTAFFSEDSRFKVATDGTITVKRHLKLHKLETSFLV
RARDSSHRELSTKVTLKSMGHHHHRHHHRDPASESNPELLMFPSVYPGLRRQKRDWVI
PPISCPENEKGEFPKNLVQIKSNRDKETKVFYSITGQGADKPPVGVFIIERETGWLKV
TQPLDREAIAKYILYSHAVSSNGEAVEDPMEIVITVTDQNDNRPEFTQEVFEGSVAEG
AVPGTSVMKVSATDADDDVNTYNAAIAYTIVSQDPELPHKNMFTVNRDTGVISVLTSG
LDRESYPTYTLVVQAADLQGEGLSTTAKAVITVKDINDNAPVFNPSTYQGQVPENEVN
ARIATLKVTDDDAPNTPAWKAVYTVVNDPDQQFVVVTDPTTNDGILKTAKGLDFEAKQ
QYILHVRVENEEPFEGSLVPSTATVTVDVVDVNEAPIFMPAERRVEVPEDFGVGQEIT
SYTAREPDTFMDQKITYRIWRDTANWLEINPETGAIFTRAEMDREDAEHVKNSTYVAL
IIATDDGSPIATGTGTLLLVLLDVNDNAPIPEPRNMQFCQRNPQPHIITILDPDLPPN
TSPFTAELTHGASVNWTIEYNDAAQESLILQPRKDLEIGEYKIHLKLADNQNKDQVTT
LDVHVCDCEGTVNNCMKAGIVAAGLQVPAILGILGGILALLILILLLLLFLRRRTVVK
EPLLPPDDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPQ
YRPRPANPDEIGNFIDENLKAADSDPTAPPYDSLLVFDYEGSGSEAASLSSLNSSESD
QDQDYDYLNEWGNRFKKLADMYGGGEDD"
regulatory complement(5112..5216)
/note="HSV-TK promoter"
/regulatory_class="promoter"
misc_feature complement(5342..6336)
/note="5' UTR of chicken beta-actin"
regulatory complement(6337..6829)
/note="Ac promoter"
/regulatory_class="promoter"
rep_origin complement(6935..7337)
/note="pMB1 ori"
CDS complement(7878..8738)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88188.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 2127 a 2402 c 2335 g 2073 t
ORIGIN
1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgggaccaaa gcggccatcg
1621 tgcctcccca ctcctgcagt tcgggggcat ggatgcgcgg atagccgctg ctggtttcct
1681 ggatgccgac ggatttgcac tgccggtaga actccgcgag gtcgtccagc ctcaggcagc
1741 agctgaacca actcgcgagg ggatcgagcc catcaaatta atacgactca ctatagggag
1801 aagcttgcat gccacgcgtc tcgagggccc ctgcaggtcg actctagagg atccccgggt
1861 accgagctcg aagaattcag gaacatgagt gactattaga atacagcaaa catggtaatt
1921 gagggcaagt acacaatgta aaaaattagt gaggtgtgag gggcttgggg ctgttgtgtc
1981 tggggcagat catagctcaa aagaacaaaa acagaaacaa acaaacaaaa aaaggcacag
2041 tttatatctc agcactgtaa aaataaaaaa taaaaaaaga tttcaacaat cagtgaaaac
2101 catcctaaca cagacagtcc agtagggaga gtgagaccac tatgcaatct gcacacttct
2161 cagatcagtc tctcttctac acactcaggg aaggagctgg aaagaccact gatataatta
2221 ttctgcatct cccatggtgc cacacggggg agacttgcta gtcccctagt cgtcctcgcc
2281 accgccgtac atgtccgcca gcttcttgaa tcggttgccc cactcgttca gataatcgta
2341 gtcctggtcc tgatccgact cagaggagtt cagtgagctc aggctagcgg cttcagaacc
2401 actgccctcg taatcgaaca ccaacagaga gtcgtaaggg ggtgccgtgg ggtcgctgtc
2461 ggctgccttc aggttttcat cgatgaagtt tccaatttca tcaggattgg caggacgggg
2521 acgatactgg ggcacgctca tgagggtggg agccacatca tttcgagtca cttccggtcg
2581 ggcatccagg cccctgtgca gctggctcaa atcaaagtcc tggtcttctt ctccacctcc
2641 ttcttcatca tagtaataca cattgtcccg ggtatcatca tctggtggca gcaggggctc
2701 tttgaccacc gttctcctcc gtagaaacag taggagcagc aggatcagaa tcagcagggc
2761 gaggatccct ccaaggattc cgaggatggc aggaacttgc aatcctgctg ccacgattcc
2821 cgccttcatg cagttgttga ccgtcccttc acagtcacac acatggacgt ccaacgtggt
2881 cacctggtct ttgttctggt tatccgcgag cttgagatgg attttgtatt cgccaatctc
2941 taagtccttt cttggttgca aaatgagaga ttcttgagct gcgtcattat actcaatggt
3001 ccagttgacg ctggccccat gggttagctc agcagtaaag ggggacgtgt tggggggaag
3061 gtctggatcc aagatggtga tgatatgagg ctgtgggttc ctctggcaga actgcatgtt
3121 tcgaggttct gggatgggag cgttgtcatt gacgtctaac aggaccagga gaagagtgcc
3181 cgtgccagtg gcaatgggtg aaccatcatc tgtggcgatg atgagagcta catatgtgct
3241 gttcttcaca tgctcagcgt cttctctgtc catctcagcg cgcgtgaaaa tggcaccagt
3301 ctctgggtta atctccagcc agttggcagt gtccctccaa atccgatacg tgatcttctg
3361 atccatgaac gtgtccggct ctcgagcggt ataagatgtg atttcctgac ccacaccaaa
3421 gtcttcgggc acttcgactc tcctctccgc aggcataaag atgggggctt cattcacgtc
3481 taccacgtcc acagtgacag tggctgtgga agggacaaga gacccctcaa agggttcctc
3541 gttctccact ctcacatgaa ggatgtattg ctgcttggcc tcaaaatcca agcccttggc
3601 tgttttcaaa atgccatcat tggtcgtggg gtctgtgacg acaacgaact gctggtcagg
3661 atcgttgact acggtgtaca cagctttcca cgccggagtg ttgggggcat catcatcggt
3721 cactttgagt gtggcgatcc gggcattgac ctcattctca ggcacttgac cctgatacgt
3781 gctcgggttg aagacaggag cgttgtcatt aatatccttg acagtgatca cagccttggc
3841 tgttgtgctc aagccttcgc cttgaaggtc agcagcctga accaccagag tgtatgtagg
3901 gtaactctct cggtccagcc cagaggtgag cacactgatg accccggtgt ccctattgac
3961 agtgaacatg tttttgtgag gcagctcagg atcctggctg acgatggtgt aggcgatggc
4021 agcgttgtag gtgttgacgt catcgtctgc atcggtggct gagaccttca tcacggaggt
4081 tcctggaaca gcgccttctg caacggatcc ctcaaacacc tcctgggtaa actctggcct
4141 gttgtcattc tgatctgtca ctgtgatcac tatctccatg ggatcctcca ccgcttcccc
4201 atttgatgac acggcatgag aatagaggat gtacttggca atggcttctc tatccagagg
4261 ctgtgtcact ttcagccagc ctgtctccct ctcaatgatg aaaacgccaa cggggggttt
4321 gtcagctcct tggccggtga tgctgtagaa aacctttgtt tctttgtccc tgttggattt
4381 gatctgaacc aggttctttg ggaattcacc cttttcattt tcggggcagc tgatgggagg
4441 gatgacccag tctcgtttct gtcttctgag acctgggtac acgctgggaa acatgagcag
4501 ctctgggttg gattcagagg cagggtcgcg gtggtggtgc cggtgatggt ggtgccccat
4561 ggacttcagc gtcactttgg tagacagctc cctatgactg gagtcccggg cgcggacgag
4621 gaaactggtc tccagcttgt ggagctttag atgccgcttc actgtgatgg tgccgtctgt
4681 cgccactttg aatcgggagt cttccgaaaa gaaggctgtc cttggccggc cggtgcatcc
4741 ttcaaatctc actctgccca ggacatggcc tctctccagg tgcctctccg gcaccgggaa
4801 ggtgtagacc tcggaactga agccgggact gcaggactca ggctccagct cctggcaaag
4861 ccatgaggag acctgcagca ggagcaggag cgcggaaaag ctgcggcacc gggctcccat
4921 ggcggacccg gcagacgccg agcaaacact gagctcgggt gcggtcgggc agggccgggt
4981 taggccggcc cgaattctat agtgtcacct aaatcgtatg tgtatgatac aaaaggttat
5041 gtattaattg taagggatct gcggcacgct gttgacgctg ttaagcgggt cgctgcaggg
5101 tcgctcggtg ttcgaggcca cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg
5161 ccgccccgac tgcatctgcg tgttcgaatt cgccaatgac aagacgctgg gcggggtttg
5221 tgtcatcata gaactaaaga catgcaaata tatttcttcc ggggacaccg ccagcaaacg
5281 cgagcaacgg gccacgggga tgaagcagaa gcttnnnnna gatcctctag agtcgacngc
5341 tggctgcgga ggaacagaga agggaagata taaaccccgc cggtcacacg ccagaagccg
5401 aaccccgccc tgccccgtcc cccccgaagg cagccgtccc cctgcggcag ccccgaggct
5461 ggagatggag aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct
5521 tcctgccggc gccgcaccgc ttcgcccgcg cccgctagag ggggtgcggc ggcgcctccc
5581 agatttcggc tccgccagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac
5641 cataaaaggc aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccggggccgc
5701 cgcgcccctc ccccgagccc tccccggccc gaggcggccc cgccccgccc ggcaccccca
5761 cctgccgcca ccccccgccc ggcacggcga gccccgcgcc acgccccgca cggagccccg
5821 cacccgaagc cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg gggttacagc
5881 ccgaccgccg cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct
5941 ttgttcccct cgcagccccc ccgcaccgcg gggcaccgcc cccggccgcg ctcccctcgc
6001 gcacacgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag
6061 cgcgggccgc acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc
6121 ccccccgcac aaagggccct cccggagccc tttaaggctt tcacgcagcc acagaaaaga
6181 aacgagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
6241 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcgcg
6301 gagcggggca cggggcgaag gcaacgcagc gactcccgcc cgccgcgcgc ttcgcttttt
6361 atagggccgc cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg
6421 attggctgcc gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc
6481 gcctggcgcg cgcccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa
6541 ataaatacaa aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg
6601 ggctcacctc gaggtgaggg tgtggggcgg gggtgtgacg cgcccctcca tcccccctcc
6661 cccctttttg attccacagt gctttgttgg ggagggggag cggggtccgc gtgtgcagcc
6721 ctccccccac ccccgaggtg gtcctgcggt ccccttgaag tcacctcccg gggagcgcca
6781 gcaggagagg cagggatagc gatgggcaat gagcgtgcat gcactgagcn nnactggctt
6841 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
6901 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac
6961 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
7021 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
7081 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
7141 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
7201 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
7261 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
7321 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
7381 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
7441 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
7501 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
7561 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
7621 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
7681 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
7741 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
7801 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
7861 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
7921 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
7981 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
8041 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
8101 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
8161 gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg
8221 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
8281 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
8341 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
8401 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
8461 atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc
8521 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
8581 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
8641 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
8701 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
8761 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
8821 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
8881 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt
8941 caagaa
//