LOCUS LT727331 7493 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector LNXCO4, complete sequence.
ACCESSION LT727331
VERSION LT727331.1
KEYWORDS .
SOURCE Mammalian expression vector LNXCO4
ORGANISM Mammalian expression vector LNXCO4
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 7493)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..7493
/organism="Mammalian expression vector LNXCO4"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4229."
/db_xref="taxon:1944743"
regulatory 145..733
/note="5LTR"
/regulatory_class="promoter"
CDS 1656..2450
/transl_table=11
/note="neomycin resistance"
/protein_id="SJL88131.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory complement(2950..3121)
/note="polyA"
/regulatory_class="terminator"
misc_feature complement(2950..4349)
/note="CO4"
misc_feature complement(3176..3637)
/note="intron"
regulatory complement(3643..4349)
/note="hCMV"
/regulatory_class="promoter"
misc_feature 4539..5132
/note="3LTR"
rep_origin complement(5490..5880)
/note="pMB1 ori"
CDS complement(6428..7288)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL88132.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1744 a 2017 c 1924 g 1808 t
ORIGIN
1 gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat
61 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag
121 ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt
181 aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat
241 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc
301 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc
361 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc
421 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa
481 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg
541 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc
601 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc
661 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg
721 ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac
781 ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc
841 tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg
901 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag
961 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac
1021 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
1081 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag
1141 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca
1201 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc
1261 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg
1321 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca
1381 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat
1441 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc
1501 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc
1561 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc
1621 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
1681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
1801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
2161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
2461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
2521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
2581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc
2641 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa
2701 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca
2761 ggagtgggga ggcacgatgg ccgctttggt cgaggcggat cctagccccc acgaaccata
2821 aaccattccc catgggggac cccgtcccta acccacgggg ccagtggcta tggcagggcc
2881 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg
2941 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga
3001 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt
3061 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
3121 ttcagttctt aagaattgcg gccgcgatcg cgttaacaga tctggccgca agcttggttg
3181 gaatctaaaa tacacaaaca attaggatcc ctgaggagca gttctttgat ttgcaccacc
3241 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc
3301 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg
3361 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat
3421 gggagacgga tccgtagttt aacacattat acactagggt cgactgtgga attgtgagcg
3481 ctcacaattc cacaacctag ggtcgactgt ggaattgtga gcgctcacaa ttccacaacc
3541 tagggtcgac tgtggaattg tgagcgctca caattccaca acctagtaaa ttttatattt
3601 accttagagc tttaaatctc tgtaggtagt ttgtccaagc ttggggccgc ggaggctgga
3661 tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc aggcgatctg
3721 acggttcact aaacgagtgt ggaattgtga gcgctcacaa ttccacactc tgcttatata
3781 gacctcccac cgtacatgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca
3841 ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg
3901 gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa
3961 ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
4021 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
4081 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
4141 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
4201 cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt
4261 aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta
4321 ctattaataa ctagtcaata atcaatgtca acatggcggt aatgttggac atgagccaat
4381 ataaatgtac atattatgat atggatacaa cgtatgcaat ggccaatagc caatattgat
4441 ttatgctata taaccaatga ataatatggc taatggccgg atccccggtg aacatcgata
4501 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
4561 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
4621 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
4681 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
4741 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
4801 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
4861 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
4921 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
4981 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
5041 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
5101 gattgactac ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc
5161 ctgcccaggg accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt
5221 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
5281 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
5341 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
5401 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
5461 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
5521 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
5581 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
5641 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
5701 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
5761 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
5821 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
5881 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
5941 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
6001 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
6061 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
6121 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
6181 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
6241 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
6301 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
6361 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
6421 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
6481 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
6541 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
6601 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
6661 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
6721 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
6781 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
6841 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
6901 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
6961 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
7021 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
7081 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
7141 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
7201 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
7261 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
7321 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
7381 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
7441 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caa
//