LOCUS LT727214 6926 bp DNA circular SYN 06-FEB-2017
DEFINITION Insect expression vector pBlueBacmsGCB1, complete sequence.
ACCESSION LT727214
VERSION LT727214.1
KEYWORDS .
SOURCE Insect expression vector pBlueBacmsGCB1
ORGANISM Insect expression vector pBlueBacmsGCB1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6926)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6926
/organism="Insect expression vector pBlueBacmsGCB1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4466."
/db_xref="taxon:1944740"
regulatory 7..95
/note="AcNPV-PH promoter"
/regulatory_class="promoter"
CDS 194..2056
/transl_table=11
/note="msGCB1"
/protein_id="SJL87775.1"
/translation="MYGFVNHALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRIIYD
DSKTYDLVAAASKVLNLNAGEILQMFGKMFFVFCQESGYDTILRVLGSNVREFLQNLD
ALHDHLATIYPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIVIGIIKTVAQQIHGT
EIDMKVIQQRNEECDHTQFLIEEKESKEEDFYEDLDRFEENGTQESRISPYTFCKAFP
FHIIFDRNLVVTQCGNAIYRVLPQLQPGNCSLLSVFSLVRPHIDISFHGILSHINTVF
VLRSKEGLLDVEKLECEDELTGAEISCLRLKGQMIYLPEADSILFLCSPSVMNLDDLT
RRGLYLSDIPLHDATRDLVLLGEQFREEYKLTQELEILTDRLQLTLRALEDEKKKTDT
LLYSVLPPSVANELRHKRPVPAKRYDNVTILFSGIVGFNAFCSKHASGEGAMKIVNLL
NDLYTRFDTLTDSRKNPFVYKVETVGDKYMTVSGLPEPCIHHARSICHLALDMMEIAG
QVQVDGESVQITIGIHTGEVVTGVIGQRMPRYCLFGNTVNLTSRTETTGEKGKINVSE
YTYRCLMSPENSDPLFHLEHRGPVSMKGKKEPMQVWFLSRKNTGTEETNEEDEN"
misc_feature 2095..2136
/note="V5 epitope"
misc_feature 2146..2163
/note="His-tag"
regulatory 2188..2262
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2188..2262)
/note="SV40 polyA late"
/regulatory_class="terminator"
CDS complement(2450..>3328)
/codon_start=1
/transl_table=11
/note="3' AcNPV ORF 1629"
/protein_id="SJL87776.1"
/translation="PETSTIVEVPTVLPKETFEPKPPSASPPPPPPPPPPPAPPAPPP
MVDLSSAPPPPPLVDLPSEMLPPPAPSLSNVLSELKSGTVRLKPAQKRPQSEIIPKSS
TTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNRPNKANTPDVKYVQALFNV
FTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKARLLLQDLASFVALSENPL
DSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIFALTYHPKDYKFNELLK
YVQQLSVNQQRTESSA"
CDS 3716..4576
/transl_table=11
/note="ampicillin"
/protein_id="SJL87777.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5117..5511
/note="pMB1 ori"
CDS complement(<5512..6624)
/transl_table=11
/note="5' lacZ fragment"
/protein_id="SJL87778.1"
/translation="MIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPS
QQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYP
ITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQD
SRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIE
NGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQD"
regulatory complement(6625..6899)
/note="AcNPV-ETL promoter"
/regulatory_class="promoter"
BASE COUNT 1787 a 1655 c 1695 g 1789 t
ORIGIN
1 gatatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
61 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
121 ccatcgggcg tgctagcgga tccgagctcg agatctgcag ctggtacccg ggtctagaat
181 tgcccttgac accatgtacg gtttcgtgaa ccatgccctg gagctgctgg tgatccgcaa
241 ttatggtccc gaggtgtggg aagacatcaa aaaagaggca cagctggatg aagaaggcca
301 gtttcttgtc agaataatat acgatgattc caaaacatat gacctggtgg ctgctgcaag
361 caaagtcctc aacctcaatg ctggcgaaat cctgcagatg tttgggaaga tgtttttcgt
421 cttctgccag gagtctggct atgataccat cttgcgtgtc ctgggatcta atgtcagaga
481 gtttttgcag aacctcgatg ccctgcatga ccacctcgcc accatttacc cagggatgcg
541 cgcgccttcc ttcaggtgca ccgatgcgga gaaaggcaaa gggctcatcc tgcactacta
601 ctcggaaaga gaggggcttc aggacatcgt gatcgggatt atcaagactg ttgctcaaca
661 gatacacggc actgagatag acatgaaggt gattcagcaa agaaatgaag aatgtgatca
721 cacccagttt ttaattgaag aaaaggaatc aaaagaagag gatttttatg aagatctgga
781 taggtttgaa gagaatggta cccaggaatc acgtatcagc ccttacacct tctgcaaagc
841 atttcctttt cacatcatat ttgaccggaa cctagtggtc actcagtgtg gcaatgccat
901 ctacagagtg ctcccccagc tccagcctgg gaactgcagc cttctgtctg tcttctctct
961 ggtccgccct cacatcgaca tcagtttcca tgggattctt tcacacatca atacagtctt
1021 tgtactgaga agcaaggaag ggttgctgga tgtggagaaa cttgagtgtg aggatgaact
1081 gactggagca gagattagct gcttacgtct caaaggccaa atgatctact taccagaagc
1141 agatagcatc ctcttcctct gttcaccaag tgtgatgaac ctggacgacc taacaagaag
1201 aggcctgtat ctgagtgaca tccctctcca cgatgctacc cgagacctgg ttcttttggg
1261 agaacagttc cgggaggagt acaaactgac acaagagctg gaaatcctca ccgacaggct
1321 gcagctcaca ctgagagcct tggaggatga gaagaaaaag acagacacat tgctgtattc
1381 tgtccttcct ccatctgttg ccaatgagct gagacacaag cgcccagtgc ctgccaaaag
1441 atacgacaat gtgaccatcc tcttcagcgg cattgtgggc ttcaatgctt tctgtagcaa
1501 gcatgcatct ggagaagggg ccatgaagat tgtcaatctc ctcaacgatc tctacacccg
1561 atttgacaca ctgactgatt cacgaaaaaa cccatttgtt tacaaggtgg aaacagttgg
1621 tgacaagtat atgacagtga gtggcttgcc agaaccttgt atccaccatg cacggtccat
1681 ttgccacctg gctttagaca tgatggaaat tgctggtcaa gttcaagtag atggtgaatc
1741 tgttcagata acaatcggga tccataccgg ggaggtggtg acaggtgtga ttggacagcg
1801 gatgcctcgg tattgtctct ttgggaatac cgtcaacctc acaagcagga cagaaaccac
1861 aggagaaaag ggaaagataa atgtttccga atatacatac aggtgtctca tgtctccaga
1921 aaactcggat ccactgttcc atttggagca cagaggccca gtgtctatga agggcaagaa
1981 ggaaccaatg caagtctggt tcctatccag gaaaaataca ggcacggagg aaacaaatga
2041 ggaggatgaa aactgagtgt caggttgtgg aagggcaatt cgaagcttag gcctggtaag
2101 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
2161 cattgacgtc tctagcttgg agtcgacaac ttgtttattg cagcttataa tggttacaaa
2221 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
2281 ggtttgtcca aactcatcaa tgtatcttat catctctcga ctctgctgaa gaggaggaaa
2341 ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt
2401 tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag
2461 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt
2521 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc
2581 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca
2641 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa
2701 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt
2761 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt
2821 cgttagtgta caattgactc gacgtaaaca cgttaaataa agcctggaca tatttaacat
2881 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag
2941 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca
3001 cgtccgcgat caaatttgta gttgagcttt ttggaattat ttctgattgc gggcgttttt
3061 gggcgggttt caatctaact gtgcccgatt ttaattcaga caacacgtta gaaagcgatg
3121 gtgcaggcgg tggtaacatt tcagacggca aatctactaa tggcggcggt ggtggagctg
3181 atgataaatc taccatcggt ggaggcgcag gcggggctgg cggcggaggc ggaggcggag
3241 gtggtggcgg tgatgcagac ggcggtttag gctcaaatgt ctctttaggc aacacagtcg
3301 gcacctcaac tattgtactg gtttcgggcg atggtgcact ctcagtacaa tctgctctga
3361 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
3421 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
3481 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
3541 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
3601 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
3661 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
3721 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
3781 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
3841 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
3901 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
3961 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
4021 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
4081 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
4141 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
4201 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
4261 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
4321 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
4381 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
4441 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
4501 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
4561 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
4621 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
4681 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
4741 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
4801 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
4861 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
4921 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
4981 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
5041 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
5101 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
5161 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
5221 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
5281 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
5341 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
5401 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
5461 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag catcctgcac
5521 catcgtctgc tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc
5581 tcgaatcagc aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg
5641 gaaaccgaca tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac
5701 caccgcacga tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag
5761 acgtagtgtg acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg
5821 cgcggtgccg ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta
5881 gtcacgcaac tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc
5941 attaaagcga gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga
6001 gacgtcacgg aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc
6061 actccagcgc agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag
6121 gtcaaattca gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca
6181 cagatgaaac gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca
6241 gctttcatca acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa
6301 cggcggattg accgtaatgg gataggtcac gttggtgtag atgggcgcat cgtaaccgtg
6361 catctgccag tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca
6421 gctttccggc accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct
6481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
6541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
6601 ttgtaaaacg acgggatcta tcatttttag cagtgattct aattgcagct gctctttgat
6661 acaactaatt ttacgacgac gatgcgagct tttattcaac cgagcgtgca tgtttgcaat
6721 cgtgcaagcg ttatcaattt ttcattatcg tattgttgca catcaacagg ctggacacca
6781 cgttgaactc gccgcagttt tgcggcaagt tggacccgcc gcgcatccaa tgcaaacttt
6841 ccgacattct gttgcctacg aacgattgat tctttgtcca ttgatcgaag cgagtgcctt
6901 cgactttttc gtgtccagtg tggctt
//