LOCUS LT727211 5683 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pEF-BOS, complete sequence.
ACCESSION LT727211
VERSION LT727211.1
KEYWORDS .
SOURCE Mammalian expression vector pEF-BOS
ORGANISM Mammalian expression vector pEF-BOS
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5683)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5683
/organism="Mammalian expression vector pEF-BOS"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4456."
/db_xref="taxon:1945010"
rep_origin 207..663
/note="M13 ori"
regulatory complement(918..1484)
/note="3' UTR of hGM-CSF, incl. polyA"
/regulatory_class="terminator"
regulatory complement(1948..3091)
/note="hEF1 promoter"
/regulatory_class="promoter"
misc_feature complement(1957..2895)
/note="intron hEF1 promoter"
CDS complement(<3132..3201)
/transl_table=11
/note="part of SV40 small t-antigen"
/protein_id="SJL87767.1"
/translation="MDKVLNREESLQLMDLLGLERSA"
rep_origin complement(3240..3315)
/note="SV40 ori"
regulatory complement(3272..3443)
/note="SV40 early promoter"
/regulatory_class="promoter"
regulatory complement(3505..3588)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(3680..4082)
/note="pMB1 ori"
CDS complement(4623..5483)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87768.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1353 a 1558 c 1474 g 1298 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatgctcg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
241 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
301 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
361 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
421 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
481 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
541 tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa
601 caggattttc gcctgctggg gcaaaccagc gtggaccgaa ttttaacaaa atattaacgt
661 tttgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
721 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
781 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
841 ccagtcacga cgttgtaaaa cgacggccag tgaattcttt tttttttttt tttttttttt
901 tttttttttt tttttttgga ggcaaacact ttattatcct gaagttcagt tacaatgtga
961 cagtggaggg gacactggtg agtggggggt gaagaggtgg agggtgacag tggagcttcc
1021 tttcacacac aggcctgaca tgattcctcc tgtctgctcc ctcccacatc cccagtcccc
1081 atccagcaag gcaaatgtcc aggcacctgc ctggccctaa aaagagtcac agtcctgacc
1141 ctcgtggggg cagggcaggt gtcccgaggc cacccagaaa aacaggagac acgtcggtga
1201 tgttcgggag tcaaaccatg tcccaaaagt cttaagaaga aaaacgagat tccccagcaa
1261 attcgtggga ccccagggcc atgcctccca gctctggcca cctctgcttg tccaggggcc
1321 tcacagccaa ggggatgcag gggccgctgt gcagtcggct gaggccagag tgaggggtgc
1381 aaggacccag atggggaaca ctgctgttta aatattaaac agggatttct tgtctcccac
1441 gtgggagacc tgatactctc aagggtccca ggtggggacc ctcactctag agtcgcggcc
1501 gcgacctgca ggcgcagaac tggtaggtat ggaagatccc tcgagatcca ttgtgctggc
1561 ggtaggcgag cagcgcctgc ctgaagctgc gggattcccg atcagaaatg agcgccagtc
1621 gtcgtcggct ctcggcaccg aagcgtatga ttctccgcca gcatggcttc ggccagtgcg
1681 tcgagcagcg cccgcttgtt cctgaagtgc cagtaaagcg ccggctgctg cacccccaac
1741 cgttccgcca gtttgcgtgt cgtcagaccg tctacgccga cctcgttcaa cagtccaggg
1801 ccggcacgga tcactgtatt cggctgcaac tttgtcatgc ttgacacttt atcactgata
1861 aacataatat gtccaccaac ttatcagtga taaagaatcc gcgccagcac aatggatctc
1921 gaggtcgagg gatctctaga gaattcctca cgacacctga aatggaagaa aaaaactttg
1981 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa
2041 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg
2101 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga
2161 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc
2221 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg
2281 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca
2341 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc
2401 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg
2461 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc
2521 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact
2581 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt
2641 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg
2701 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga
2761 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt
2821 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
2881 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
2941 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
3001 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
3061 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc
3121 ggagcctcac gaggcactcc tttcaagacc tagaaggtcc attagctgca aagattcctc
3181 tctgtttaaa actttatcca tctttgcaaa gctttttgca aaagcctagg cctccaaaaa
3241 agcctcctca ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa
3301 taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg
3361 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
3421 tacttctgcc tgctggggag cctaagcttg gcgtaatcat ggtcatagct gtttcctgtg
3481 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
3541 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
3601 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
3661 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
3721 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
3781 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
3841 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
3901 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
3961 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
4021 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
4081 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
4141 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
4201 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
4261 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
4321 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
4381 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
4441 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
4501 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
4561 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
4621 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
4681 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
4741 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
4801 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
4861 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
4921 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
4981 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
5041 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
5101 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
5161 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
5221 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
5281 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
5341 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
5401 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
5461 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
5521 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
5581 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
5641 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
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