LOCUS LT727201 6071 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-2, complete sequence.
ACCESSION LT727201
VERSION LT727201.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-2
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-2
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6071)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6071
/organism="Mammalian expression vector pCAGGS-E-mABIN-2"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4370."
/db_xref="taxon:1944804"
misc_feature 13..51
/note="E-tag"
CDS 67..1359
/transl_table=11
/note="mABIN-2"
/protein_id="SJL87747.1"
/translation="MSSGDPRSGRQDGAPRAAAALCGLYHEAGQQLQRLKDQLAARDA
LIASLRTRLAALEGHTAPSLVDALLDQVERFREQLRRQEEGASETQLRQEVERLTERL
EEKEREMQQLMSQPQHEQEKEVVLLRRSVAEKEKARAASDVLCRSLADETHQLRRTLA
ATAHMCQHLAKCLDERQCAQGDAGEKSPAELEQTSSDASGQSVIKKLQEENRLLKQKV
THVEDLNAKWQRYDASRDEYVKGLHAQLKRRQVPLEPELMKKEISRLNRQLEEKISDC
AEANQELTAMRMSRDTALERVQMLEQQILAYKDDFKSERADRERAHSRIQELEEKIMS
LMYQVSQRQDSREPGPCRIHTGNKTAKYLEMDALEHVTPGGWRPESRSQQMEPSAEGG
HVCTAQRGQGDLQCPHCLRCFSDEQGEAFLRHLSECCQ"
regulatory 1361..1452
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1453..1809
/note="3' FR of beta-globin"
regulatory complement(1887..1969)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2111..2186
/note="SV40 ori"
regulatory 2216..2291
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2216..2291)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2406..2807)
/note="pMB1 ori"
CDS complement(3348..4208)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87748.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4344..4721
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4727..6068
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5096..6020
/note="intron AG promoter"
BASE COUNT 1329 a 1582 c 1753 g 1407 t
ORIGIN
1 ttcctcgaga tgggtgcgcc ggtgccgtat ccggatccgc tggaaccgcg tgaattcggg
61 atccgtatgt cgtctgggga cccaaggtct ggtagacagg acggggcccc gcgtgcggcc
121 gcagcgctct gtggcctgta ccacgaggcc ggccagcaac tacagcgcct gaaggatcag
181 ctggccgcgc gtgacgccct catcgcgagc ctccgcaccc gcctcgcggc tctggaaggg
241 cacacggcgc cgtcactcgt ggacgcactt ctggatcagg tggagcgctt ccgtgagcag
301 ctgcgacgac aggaggaagg cgcttcggag acccagctgc ggcaggaagt tgaaagactt
361 acggagcgtc tagaggaaaa agagagggag atgcaacagc tgatgagcca gcctcagcat
421 gagcaagaga aggaggtagt cttgcttcgg cgaagtgtgg cagagaagga gaaagccagg
481 gccgccagtg atgttctgtg ccgctccttg gctgatgaga cccaccaact gcgcaggaca
541 ttggcagcca ctgcccacat gtgccaacat ctggccaaat gtctggatga acgacagtgt
601 gcacagggag acgctgggga gaaaagccct gctgagctag agcaaacaag cagcgatgct
661 tctggccaga gtgttattaa gaagttacag gaagaaaatc gactgttaaa acagaaggtg
721 actcatgtag aagacctcaa tgctaagtgg cagcgttatg atgcaagtag ggacgaatat
781 gtgaaggggt tgcatgccca gctaaagagg cggcaggtcc ctctggagcc tgagctgatg
841 aagaaggaga tttcccgact taacagacag ttggaggaga aaataagtga ctgtgcggaa
901 gcaaaccagg agctgacagc catgaggatg tcccgggaca ctgcgctgga gcgagtgcag
961 atgctagaac agcagattct tgcttacaag gatgacttca aatcagaaag ggcagatcgg
1021 gaacgagcgc acagtaggat tcaagagctg gaggaaaaga tcatgtcctt gatgtaccaa
1081 gtgtcccaga gacaggactc ccgggagcca ggaccctgtc ggattcatac ggggaacaaa
1141 actgccaagt acttagagat ggatgcactg gagcatgtga cccctggcgg ctggaggcct
1201 gagtctaggt cccaacagat ggaaccttct gcagagggtg ggcatgtgtg cacagcccag
1261 agaggtcagg gtgaccttca gtgccctcat tgcctgcggt gcttcagtga tgagcaaggc
1321 gaggcattcc tcaggcacct gtctgagtgc tgccaatgaa gatctttttc cctctgccaa
1381 aaattatggg gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt
1441 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
1501 gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc
1561 ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca
1621 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
1681 tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
1741 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
1801 atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
1861 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
1921 aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
1981 tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata
2041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
2101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
2161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact
2221 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
2281 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
2341 atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
2401 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
2461 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag ggataacgca
2521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
2581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
2641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
2701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
2761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
2821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
2881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
2941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
3001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
3061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
3121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
3181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
3241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
3301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
3361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
3421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
3481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
3541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
3601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
3661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
3721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
3781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
3841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
3901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
3961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
4021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
4081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
4141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
4201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
4261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
4321 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
4381 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
4441 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
4501 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
4561 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
4621 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
4681 tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac
4741 gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat
4801 tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc ccccaggcgg
4861 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg cagccaatca
4921 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
4981 aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg tgccccgctc
5041 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
5101 cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat gacggcttgt
5161 ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt tgtgcggggg
5221 gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc
5281 gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt
5341 gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa
5401 caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt
5461 cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc cggcttcggg
5521 tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca
5581 ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg gggaaggggc
5641 gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt
5701 tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga
5761 aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg
5821 gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc
5881 ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg
5941 cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg
6001 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
6061 ttggcaaaga a
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