LOCUS LT727155 6293 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-EYopH, complete sequence.
ACCESSION LT727155
VERSION LT727155.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-EYopH
ORGANISM Mammalian expression vector pCAGGS-EYopH
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6293)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6293
/organism="Mammalian expression vector pCAGGS-EYopH"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4663."
/db_xref="taxon:1944822"
misc_feature 16..54
/note="E-tag"
CDS <64..1467
/codon_start=1
/transl_table=11
/note="YopH"
/protein_id="SJL87615.1"
/translation="NLSLSDLHRQVSRLVQQESGDCTGKLRGNVAANKETTFQGLTIA
SGARESEKVFAQTVLSHVANIVLTQEDTAKLLQSTVKHNLNNYELRSVGNGNSVLVSL
RSDQMTLQDAKVLLEAALRQESGARGHVSSHSHSVLHAPGTPVREGLRSHLDPRTPPL
PPRERPHTSGHHGAGEARATAPSTVSPYGPEARAELSSRLTTLRNTLAPATNDPRYLQ
ACGGEKLNRFRDIQCCRQTAVRADLNANYIQVGNTRTIACQYPLQSQLESHFRMLAEN
RTPVLAVLASSSEIANQRFGMPDYFRQSGTYGSITVESKMTQQVGLGDGIMADMYTLT
IREAGQKTISVPVVHVGNWPDQTAVSSEVTKALASLVDQTAETKRNMYESKGSSAVAD
DSKLRPVIHCRAGVGRTAQLIGAMCMNDSRNSQLSVEDMVSQMRVQRNGIMVQKDEQL
DVLIKLAEGQGRPLLNS"
regulatory 1583..1674
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1675..2031
/note="3' FR of beta-globin"
regulatory complement(2109..2191)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2333..2408
/note="SV40 ori"
regulatory 2439..2513
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2439..2513)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2628..3029)
/note="pMB1 ori"
CDS complement(3570..4430)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87616.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4566..4943
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4949..6290
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5818..6242
/note="intron AG promoter"
BASE COUNT 1432 a 1622 c 1709 g 1530 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gctaacttat cattaagcga tcttcatcgt caggtatctc gattggtgca gcaagagagc
121 ggtgattgta ccgggaaatt aagaggtaac gttgctgcca ataaagaaac tacctttcaa
181 ggtttgacca tagccagtgg tgccagagag tcagaaaaag tatttgctca aactgtacta
241 agccacgtag caaatattgt tctaactcaa gaagataccg ctaagctatt gcaaagcacg
301 gtaaagcata atttgaataa ttatgaatta agaagtgtcg gcaatggtaa tagtgtactt
361 gtcagtttac gtagtgacca aatgacacta caagacgcca aagtgctgtt ggaggctgca
421 ttgcgacaag agtcgggagc gagggggcat gtatcatctc attcacattc agtccttcac
481 gcaccgggaa ccccggtgcg tgaaggactg cgttcacatc tagaccccag aacaccaccg
541 ttgccaccgc gtgaacgacc acacacttct ggccatcacg gggctggcga agccagagcc
601 accgcaccaa gcactgtttc tccttatggc ccagaagcgc gcgcagaact cagcagccgc
661 ctcaccacat tgcgcaatac gctggcgcca gcaacgaatg atccgcgtta cttacaagcc
721 tgcggcggtg aaaagctaaa ccgatttaga gatattcaat gctgtcggca aaccgcagta
781 cgcgccgatc ttaatgccaa ttacatccag gtcggtaaca ctcgtaccat agcgtgccag
841 tatccgctac aatctcaact tgaaagccat ttccgtatgc tggcagaaaa ccgaacgcca
901 gtgttggctg ttttagcgtc cagttctgag atagccaatc aaagattcgg tatgccagat
961 tatttccgcc agagtggtac ctatggcagt atcactgtag agtctaaaat gactcagcaa
1021 gttggtctcg gtgacgggat tatggcagat atgtatactt taacgattcg tgaagcgggt
1081 caaaaaacaa tttctgttcc tgtggttcat gttggcaatt ggcccgatca gaccgcagtc
1141 agctctgaag ttaccaaggc actcgcttca ctggtagatc aaacagcaga aacaaaacgc
1201 aatatgtatg aaagcaaagg aagttcagcg gtagcagatg actccaaatt acggccggta
1261 atacattgcc gtgcgggtgt tggccgtact gcgcaactga ttggcgcaat gtgcatgaat
1321 gatagtcgta atagtcagtt aagcgtagaa gatatggtca gccaaatgcg agtacaaaga
1381 aatggtatta tggtacaaaa agatgagcaa cttgatgttc tgattaagtt ggctgaagga
1441 caagggcgac cattattaaa tagctaaccc gggacattaa acgcgtgggt accggatccg
1501 gcaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
1561 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
1621 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
1681 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
1741 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
1801 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
1861 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
1921 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
1981 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
2041 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
2101 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg
2161 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
2221 gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
2281 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
2341 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
2401 ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata
2461 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
2521 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca
2581 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
2641 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
2701 aaaggcggta atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca
2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
3241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
3541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
3601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
3661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
3721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
3781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
3841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
3901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
3961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
4021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
4081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
4141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
4201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
4261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
4321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
4381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
4561 gggtcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
4621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
4681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
4741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
4801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
4861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
4921 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
4981 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
5041 agcgatgggg gcgggggggg ggggggggcc ccccccaggc ggggcggggc ggggcgaggg
5101 gcggggcggg gcgaggcgga aaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
5161 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
5221 ggcgggagtc gttgcgcgct gccttccccc cgtgccccgc tccgccgccg cctcgcgccg
5281 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
5341 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
5401 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
5461 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
5521 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
5581 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
5641 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
5701 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
5761 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
5821 ggcggggccg cctcgggccg gggagggctc gggggaaggg gcgcggcggc ccccggagcg
5881 ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga
5941 gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc
6001 gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
6061 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct
6121 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
6181 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
6241 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaa
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