LOCUS LT727106 6261 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-CrmA-T291R, complete sequence.
ACCESSION LT727106
VERSION LT727106.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-CrmA-T291R
ORGANISM Mammalian expression vector pCAGGS-CrmA-T291R
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6261)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6261
/organism="Mammalian expression vector pCAGGS-CrmA-T291R"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 4577."
/db_xref="taxon:1944757"
misc_feature 4..296
/note="5' UTR of cowpox virus crmA"
CDS 297..1322
/transl_table=11
/note="cowpox virus crmA mutant"
/protein_id="SJL87444.1"
/translation="MDIFREIASSMKGENVFISPPSISSVLTILYYGANGSTAEQLSK
YVEKEADKNKDDISFKSMNKVYGRYSAVFKDSFLRKIGDNFQTVDFTDCRTVDAINKC
VDIFTEGKINPLLDEPLSPDTCLLAISAVYFKAKWLMPFEKEFTSDYPFYVSPTEMVD
VSMMSMYGEAFNHASVKESFGNFSIIELPYVGDTSMVVILPDNIDGLESIEQNLTDTN
FKKWCDSMDAMFIDVHIPKFKVTGSYNLVDALVKLGLTEVFGSTGDYSNMCNSDVSVD
AMIHKTYIDVNEEYREAAAATCALVADCASTVTNEFCADHPFIYVIRHVDGKILFVGR
YCSPTTN"
misc_feature 1167..1175
/note="T291R"
misc_feature 1323..1468
/note="3' UTR of cowpox virus crmA"
regulatory 1551..1642
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1643..1999
/note="3' flanking region of rabbit beta-globin"
regulatory complement(2077..2159)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2301..2376
/note="SV40 ori"
regulatory complement(2407..2481)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 2407..2481
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2596..2997)
/note="pMB1 ori"
CDS complement(3538..4398)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL87445.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4534..4911
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4917..6258
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5286..6210
/note="intron AG promoter"
BASE COUNT 1492 a 1500 c 1625 g 1644 t
ORIGIN
1 ttcccatgga agaacgaaag tagtataaaa gtaataaaac aaaaaaaaga atataaaaaa
61 tttatagcca ctttctttga ggactgtttt cctgaaggaa atgaacctct ggaattagtt
121 agatatatag aattagtata cacgctagat tattctcaaa ctcctaatta tgacagacta
181 cgtagactgt ttatacaaga ttgaaaatat atttcttttt attgagtggt ggtagttacg
241 gatatctaat attaatatta gactatctct atcgtcacac aacaaaatcg attgccatgg
301 atatcttcag ggaaatcgca tcttctatga aaggagagaa tgtattcatt tctccaccgt
361 caatctcgtc agtattgaca atactgtatt atggagctaa tggatccact gctgaacagc
421 tatcaaaata tgtagaaaag gaggcggaca agaataagga tgatatctca ttcaagtcca
481 tgaataaagt atatgggcga tattctgcag tgtttaaaga ttcctttttg agaaaaattg
541 gagataattt ccaaactgtt gacttcactg attgtcgcac tgtagatgcg atcaacaagt
601 gtgttgatat cttcactgag gggaaaatta atccactatt ggatgaacca ttgtctccag
661 atacctgtct cctagcaatt agtgccgtat actttaaagc aaaatggttg atgccatttg
721 aaaaggaatt taccagtgat tatccctttt acgtatctcc aacggaaatg gtagatgtaa
781 gtatgatgtc tatgtacggc gaggcattta atcacgcatc tgtaaaagaa tcattcggca
841 acttttcaat catagaactg ccatatgttg gagatactag tatggtggta attcttccag
901 acaatattga tggactagaa tccatagaac aaaatctaac agatacaaat tttaagaaat
961 ggtgtgactc tatggatgct atgtttatcg atgtgcacat tcccaagttt aaggtaacag
1021 gctcgtataa tctggtggat gcgctagtaa agttgggact gacagaggtg ttcggttcaa
1081 ctggagatta tagcaatatg tgtaattcag atgtgagtgt cgacgctatg atccacaaaa
1141 cgtatataga tgtcaatgaa gagtatcgcg aagcggctgc agcaacttgt gcgctggtgg
1201 cagactgtgc atcaacagtt acaaatgagt tctgtgcaga tcatccgttc atctatgtga
1261 ttaggcatgt cgatggcaaa attcttttcg ttggtagata ttgctctcca acaactaatt
1321 aaatcacatt cttaatatta gaatattaga atattatata gttaagattt ttactaattg
1381 gttaaccatt tttttaaaaa aatagaaaaa aaacatgtta tattagcgag ggtcgttatt
1441 cttccaattg caattggtaa gatgacgggg aattcactcc tcaggtgcag gctgcctatc
1501 agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga gatctttttc
1561 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
1621 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
1681 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
1741 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
1801 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
1861 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
1921 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
1981 ctcttctctt atggagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
2041 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
2101 ataaagtgta aagcctgggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
2161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
2221 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
2281 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
2341 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
2401 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
2461 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
2521 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
2581 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
2641 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
2701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
2761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
2821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
2881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
2941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
3001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
3061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
3121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
3181 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
3241 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
3301 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
3361 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
3421 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
3481 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
3541 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
3601 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
3661 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
3721 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
3781 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
3841 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
3901 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
3961 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
4021 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
4081 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
4141 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
4201 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
4261 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
4321 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
4381 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
4441 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
4501 gcgcacattt ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat
4561 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
4621 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
4681 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
4741 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
4801 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
4861 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
4921 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
4981 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcccc
5041 ccccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaaa ggtgcggcgg
5101 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
5161 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgt tgcgcgctgc cttccccccg
5221 tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc
5281 cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc ttggtttaat
5341 gacggcttgt ttcttttctg tggctgcgtg aaagccttga ggggctccgg gagggccctt
5401 tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt gtgcgtgggg agcgccgcgt
5461 gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg ctttgtgcgc
5521 tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc ggggggggct
5581 gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg ggggtgagca gggggtgtgg
5641 gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc ccgagttgct gagcacggcc
5701 cggcttcggg tgcggggctc cgtacggggc gtggcgcggg gctcgccgtg ccgggcgggg
5761 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
5821 gggaaggggc gcggcggccc ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc
5881 cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt
5941 gcggagccga aatctgggag gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg
6001 tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt
6061 ccccttctcc ctctccagcc tcggggctgt ccgcgggggg acggctgcct tcggggggga
6121 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
6181 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg
6241 tctcatcatt ttggcaaaga a
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