LOCUS LT727026 5518 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(469-647), complete
sequence.
ACCESSION LT727026
VERSION LT727026.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(469-647)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(469-647)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5518)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5518
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(469-647)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5372."
/db_xref="taxon:1944801"
misc_feature 16..54
/note="E-tag"
CDS 64..603
/transl_table=11
/note="mABIN-1(469-647)"
/protein_id="SJL87225.1"
/translation="MTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQA
QVTLTNAQLKTLKEEEKAKEALKQQKRKAKASGERYHMEPHPEHVCGAYPYAYPPMPA
MVPHHAYKDWSQIRYPPPPVPMEHPPPHPNSRLFHLPEYTWRPPCAGIRNQSSQVMDP
PPDRPAEPESADNDCDGPQ"
regulatory 808..899
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 900..1256
/note="3' FR of beta-globin"
regulatory complement(1334..1416)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1558..1633
/note="SV40 ori"
regulatory complement(1664..1738)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1664..1738
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1853..2254)
/note="pMB1 ori"
CDS complement(2795..3655)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87226.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3791..4168
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4174..5515
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4543..5467
/note="intron AG promoter"
BASE COUNT 1190 a 1495 c 1528 g 1305 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag
121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta
181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct caaagaggag
241 gagaaggcca aggaagccct caaacagcag aagaggaaag caaaggcttc gggagagcgc
301 taccacatgg aaccccaccc tgagcacgtc tgcggcgcct atccctatgc ctacccaccc
361 atgccagcca tggtacctca ccatgcctac aaggactggt cccagatccg ataccctcca
421 ccccctgtgc ccatggagca cccgccccca caccccaact ctcgcctctt ccatctgccg
481 gagtacacct ggcgtccacc ctgtgcaggg attcggaatc agagctctca agtgatggac
541 ccgcccccag acaggcctgc agagccagag tctgcagaca atgactgtga tgggccccag
601 tgaggctgca gtgggtcatt tggttccacc ttcatctttc agagccagct gacctcagat
661 tgccaaaagt ttgaaggcca tgtgcatgtt ctgtgtgacc caagccttgg cagaggagag
721 gctgggatgg gtagctggct cacatcccca gccaagcctc gaactgttga caagaccccc
781 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
841 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
901 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
961 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
1021 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
1081 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
1141 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
1201 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
1261 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
1321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc
1381 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
1441 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
1501 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
1561 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
1621 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
1681 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
1741 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
1801 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
1861 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
1921 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt
1981 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
2041 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
2101 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
2161 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
2221 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
2281 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
2341 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
2401 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
2461 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
2521 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
2581 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
2641 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
2701 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
2761 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
2821 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
2881 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
2941 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
3001 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
3061 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
3121 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
3181 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
3241 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
3301 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
3361 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
3421 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
3481 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
3541 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
3601 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
3661 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
3721 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
3781 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
3841 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
3901 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
3961 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
4021 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
4081 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
4141 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
4201 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
4261 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc
4321 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc
4381 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
4441 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg
4501 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
4561 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
4621 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
4681 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
4741 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
4801 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
4861 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
4921 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
4981 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
5041 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg
5101 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
5161 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
5221 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
5281 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
5341 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
5401 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
5461 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaa
//