LOCUS LT727018 5220 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(388-524), complete
sequence.
ACCESSION LT727018
VERSION LT727018.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(388-524)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(388-524)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5220)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5220
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(388-524)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5364."
/db_xref="taxon:1944787"
misc_feature 16..54
/note="E-tag"
CDS <64..477
/codon_start=1
/transl_table=11
/note="mABIN-1 (388-524)"
/protein_id="SJL87209.1"
/translation="EMEETDKEQLTAEAKELRQKVRYLQDQLSPLTRQREYQEKEIQR
LNKALEEALSIQASPSSPPAAFGSPEGVGGHLRKQELVTQNELLKQQVKIFEEDFQRE
RSDRERMNEEKEELKKQVEKLQAQVTLTNAQLKTL"
regulatory 510..601
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 602..958
/note="3' FR of beta-globin"
regulatory complement(1036..1118)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1260..1335
/note="SV40 ori"
regulatory complement(1366..1440)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1366..1440
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1555..1956)
/note="pMB1 ori"
CDS complement(2497..3357)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87210.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3493..3870
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3876..5217
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4245..5169
/note="intron AG promoter"
BASE COUNT 1130 a 1382 c 1465 g 1243 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagagatgg aagagaccga caaggagcag ctgacagcag aggccaagga actgcgccag
121 aaggtcaggt acctacagga tcagctgagc ccgctcacaa ggcaacgaga ataccaggag
181 aaggagatcc agcggctcaa taaggccctg gaggaggccc tcagcatcca ggcctctcca
241 tcatctccgc ctgcagcttt tgggagtcca gaaggcgttg ggggccatct gaggaagcag
301 gaactagtga cacagaatga gttgctgaaa cagcaggtaa agatctttga agaggacttc
361 cagagggaac ggagtgaccg tgaacgcatg aatgaagaga aggaggagct gaagaagcaa
421 gtagagaagc tgcaggccca ggtcaccctg actaatgccc agctcaaaac tctctgaccc
481 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
541 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
601 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
661 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
721 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
781 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
841 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
901 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
961 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
1021 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctgggtg
1081 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
1141 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
1201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
1261 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
1321 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt gtttattgca
1381 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
1441 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
1501 cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
1561 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
1621 ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag gaaagaacat
1681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
1741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
1801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
1861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
1921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
1981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
2041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
2101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
2161 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
2221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
2281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
2341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
2401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
2461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
2521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
2581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
2641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
2701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
2761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
2821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
2881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
2941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
3001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
3061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
3121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
3181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
3241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
3301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
3361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
3421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
3481 gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
3541 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
3601 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
3661 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
3721 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
3781 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
3841 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
3901 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
3961 tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg gcggggcggg
4021 gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag agcggcgcgc
4081 tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg
4141 cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct
4201 cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg
4261 gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt
4321 ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg
4381 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg
4441 gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga
4501 gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg
4561 tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac
4621 cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc
4681 gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc
4741 cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg cggcggcccc
4801 cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg
4861 tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg
4921 cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa
4981 atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct
5041 cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc
5101 ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt
5161 ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa
//