LOCUS LT726960 6271 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hPELI3, complete sequence.
ACCESSION LT726960
VERSION LT726960.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hPELI3
ORGANISM Mammalian expression vector pCAGGS-E-hPELI3
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6271)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6271
/organism="Mammalian expression vector pCAGGS-E-hPELI3"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5201."
/db_xref="taxon:1944777"
misc_feature 11..49
/note="E-tag"
CDS 59..1468
/transl_table=11
/note="hPELI3"
/protein_id="SJL87062.1"
/translation="MVLEGNPEVGSPRTSDLQHRGNKGSCVLSSPGEDAQPGEEPIKY
GELIVLGCCEEGGEETEAQRGEVTGPRAHSCYNGCLASGDKGRRRSRLALSRRSHANG
VKPDVMHHISTPLVSKALSNRGQHSISYTLSRSHSVIVEYTHDSDTDMFQIGRSTENM
IDFVVTDTSPGGGAAEGPSAQSTISRYACRILCDRRPPYTARIYAAGFDASSNIFLGE
RAAKWRTPDGLMDGLTTNGVLVMHPAGGFSEDSAPGVWREISVCGNVYTLRDSRSAQQ
RGKLVENESNVLQDGSLIDLCGATLLWRTPAGLLRAPTLKQLEAQRQEANAARPQCPV
GLSTLAFPSPARGRTAPDKQQPWVYVRCGHVHGYHGWGCRRERGPQERECPLCRLVGP
YVPLWLGQEAGLCLDPGPPSHAFAPCGHVCSEKTARYWAQTPLPHGTHAFHAACPFCG
AWLTGEHGCVRLIFQGPLD"
regulatory 1556..1647
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 1648..2004
/note="3' FR of beta-globin"
regulatory complement(2082..2164)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 2306..2381
/note="SV40 ori"
regulatory 2412..2486
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(2412..2486)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2601..3002)
/note="pMB1 ori"
CDS complement(3543..4403)
/transl_table=11
/note="ampicillin"
/protein_id="SJL87063.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4539..4916
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4922..6263
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 5291..6215
/note="intron AG promoter"
BASE COUNT 1271 a 1760 c 1799 g 1441 t
ORIGIN
1 ttccaccatg ggtgcgccgg tgccgtatcc ggacccgctg gaaccgcgtg cggccgcaat
61 ggtgctggaa ggaaaccctg aagtggggtc cccccgaacc tcagacctcc agcaccgggg
121 gaacaagggc tcttgcgttc tctcctctcc cggtgaagat gcgcagccag gcgaggagcc
181 catcaagtat ggtgaactca tcgtcctggg atgctgtgag gaaggaggtg aggaaaccga
241 ggctcagaga ggggaagtga ctggcccaag ggcacacagc tgctacaatg gttgtctggc
301 aagtggggac aagggccgcc ggcgaagccg cctggcactg agccgccggt cgcacgccaa
361 cggggtgaag ccagacgtca tgcaccacat ctccacgccg ctcgtctcca aggcactgag
421 taaccgtggt cagcacagca tctcgtatac actgtcccgg agccactcgg tcatagtgga
481 gtatacacat gatagcgaca cagacatgtt ccagattggc cgctccacag agaacatgat
541 tgacttcgtg gtaacagaca cgtcccctgg aggaggggct gccgagggcc cttctgccca
601 gagcaccatc tcccgctatg cctgccgcat cctctgtgac cgccggccac cctatactgc
661 ccgcatctat gccgctggct tcgacgcctc tagcaacatc ttccttggag agcgagcggc
721 caaatggcgg accccagatg gcctgatgga tggactgacc accaatggag tcctggtgat
781 gcacccggca ggcggcttct ccgaggactc agccccgggt gtctggcggg agatctcggt
841 ctgtgggaat gtgtacacat tgcgggacag ccgctcagcc cagcagcggg gcaagctggt
901 ggaaaacgag tccaacgtgc tgcaggacgg ctctctcatc gacctgtgtg gggccacact
961 gctgtggcgc acaccggcgg ggctgctgcg ggctcccaca ctgaagcaac tggaggccca
1021 gcggcaggag gcaaatgcag cgcggcccca gtgccccgtg ggcctcagca ctctggcctt
1081 ccccagccca gcccgtggcc gcacagcgcc cgacaaacag cagccctggg tctacgtccg
1141 ctgcgggcac gtccatggct accacggctg gggctgccgg cgggagcggg gcccccagga
1201 gcgcgaatgt cctctctgcc gccttgtggg gccttatgtg cctctatggc ttggccagga
1261 ggccggcctc tgcctggacc ctgggccgcc tagccatgcc tttgcacctt gcggccacgt
1321 ctgctctgag aagactgccc gctactgggc ccagacacca ctgccccacg gcacccatgc
1381 tttccatgcc gcctgcccct tttgcggggc ctggcttacc ggcgagcatg gctgcgtccg
1441 cctcattttc cagggcccgc tggattagct cgaggaattc actcctcagg tgcaggctgc
1501 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
1561 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
1621 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
1681 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
1741 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
1801 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
1861 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
1921 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
1981 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
2041 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
2101 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
2161 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
2221 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
2281 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
2341 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
2401 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
2461 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
2521 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
2581 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
2641 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
2701 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
2761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
2821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
2881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
2941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
3001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
3061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
3121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
3181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
3241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
3301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
3361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
3421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
3481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
3541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
3601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
3661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
3721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
3781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
3841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
3901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
3961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
4021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
4081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
4141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
4201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
4261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
4321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
4381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
4441 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
4501 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
4561 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
4621 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
4681 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
4741 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
4801 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
4861 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
4921 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
4981 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
5041 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
5101 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
5161 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
5221 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
5281 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
5341 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
5401 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
5461 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
5521 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
5581 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
5641 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
5701 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
5761 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
5821 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
5881 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
5941 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
6001 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
6061 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
6121 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
6181 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
6241 tgctgtctca tcattttggc aaagaattga a
//