LOCUS LT726920 7910 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCD-MK, complete sequence. ACCESSION LT726920 VERSION LT726920.1 KEYWORDS . SOURCE Mammalian expression vector pCD-MK ORGANISM Mammalian expression vector pCD-MK other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7910) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7910 /organism="Mammalian expression vector pCD-MK" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5536." /db_xref="taxon:1944916" regulatory 235..818 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory 863..879 /note="T7 promoter" /regulatory_class="promoter" CDS 933..>3404 /transl_table=11 /note="hMLT" /protein_id="SJL86942.1" /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQT TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE IIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHL VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF SRSNVPVETTDEIPFSFSDRLRISEK" misc_feature 936..938 /note="Kozak" misc_feature 2418..2420 /note="stability creator" misc_feature 2880..2882 /note="PCR artefact" misc_feature 3414..3443 /note="Myc epitope" misc_feature 3459..3476 /note="His-tag" regulatory 3505..3729 /note="BGH polyA" /regulatory_class="terminator" rep_origin 3775..4203 /note="f1 ori" regulatory 4208..4489 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 4446..4521 /note="SV40 ori" CDS 4613..5407 /transl_table=11 /note="Tn5 neomycin resistance" /protein_id="SJL86943.1" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 5583..5657 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(5583..5657) /note="SV40 polyA late" /regulatory_class="terminator" regulatory complement(5796..5879) /note="lac promoter" /regulatory_class="promoter" rep_origin complement(5971..6373) /note="pMB1 ori" CDS complement(6914..7774) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86944.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1979 a 1918 c 1968 g 2045 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatcctgcca ccatggcgct gttgggggac ccgctacagg 961 ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc 1021 tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg 1081 agggccgggg ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca 1141 gttgcctaga cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc 1201 tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc 1261 tgcaggctat ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta 1321 ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg 1381 caactggaca tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg 1441 gaaatacatc agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct 1501 gtcgagttaa taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg 1561 acatcccaga gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct 1621 gtgttgaacc aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg 1681 ctgttggaag ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg 1741 agaccaaaaa gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt 1801 gtcatgtata taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa 1861 gaacagatga ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata 1921 ataaagagca aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa 1981 atatgaatta ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga 2041 ctaacttact gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg 2101 agatgcgtaa tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat 2161 tatattatgc aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg 2221 ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc 2281 aagaaaaaga aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact 2341 acgatgatac cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat 2401 atgccacgtg tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa 2461 tctttatgaa atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg 2521 atgaagttgc agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga 2581 ttcgaagtag tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt 2641 ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta 2701 tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca 2761 atgtcatgat catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg 2821 cctacgttac tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca 2881 cgcctgaaga aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata 2941 ccagactcag ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat 3001 atcagtactc aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac 3061 ctctcattgc taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt 3121 gtcttatgtc taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt 3181 atcactcatt gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta 3241 atgttacacc agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt 3301 tcgctcacca tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg 3361 aaataccatt tagtttctct gacaggctca gaatttctga aaaagggccc ttcgaacaaa 3421 aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag 3481 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 3721 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 3781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 3901 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 3961 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 4021 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 4081 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4141 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4201 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4501 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4561 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 4621 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 4681 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 4741 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 4801 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 4861 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 4921 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 4981 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 5041 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 5101 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 5161 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 5221 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 5281 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 5341 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 5401 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat 5461 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc 5521 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc 5581 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5701 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc 5761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 5821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 5881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 5941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc 6001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt 6061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 6121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 6181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 6241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 6301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 6361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 6421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 6481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 6541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 6601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 6661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 6721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 6781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 6841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 6901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 6961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 7801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 7861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //