LOCUS       LT726920                7910 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCD-MK, complete sequence.
ACCESSION   LT726920
VERSION     LT726920.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCD-MK
  ORGANISM  Mammalian expression vector pCD-MK
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Mammalian expression vector pCD-MK"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 5536."
                     /db_xref="taxon:1944916"
     regulatory      235..818
                     /note="hCMV-IE promoter and enhancer"
                     /regulatory_class="promoter"
     regulatory      863..879
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     CDS             933..>3404
                     /transl_table=11
                     /note="hMLT"
                     /protein_id="SJL86942.1"
                     /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL
                     LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT
                     VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW
                     FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS
                     VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM
                     VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQT
                     TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR
                     NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ
                     EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN
                     GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG
                     TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE
                     IIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHL
                     VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS
                     AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF
                     SRSNVPVETTDEIPFSFSDRLRISEK"
     misc_feature    936..938
                     /note="Kozak"
     misc_feature    2418..2420
                     /note="stability creator"
     misc_feature    2880..2882
                     /note="PCR artefact"
     misc_feature    3414..3443
                     /note="Myc epitope"
     misc_feature    3459..3476
                     /note="His-tag"
     regulatory      3505..3729
                     /note="BGH polyA"
                     /regulatory_class="terminator"
     rep_origin      3775..4203
                     /note="f1 ori"
     regulatory      4208..4489
                     /note="SV40 early promoter"
                     /regulatory_class="promoter"
     rep_origin      4446..4521
                     /note="SV40 ori"
     CDS             4613..5407
                     /transl_table=11
                     /note="Tn5 neomycin resistance"
                     /protein_id="SJL86943.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      5583..5657
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(5583..5657)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     regulatory      complement(5796..5879)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(5971..6373)
                     /note="pMB1 ori"
     CDS             complement(6914..7774)
                     /transl_table=11
                     /note="ampicillin resistance"
                     /protein_id="SJL86944.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT         1979 a         1918 c         1968 g         2045 t
ORIGIN      
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatcctgcca ccatggcgct gttgggggac ccgctacagg
      961 ccctgccgcc ctcggccgcc cccacggggc cgctgctcgc ccctccggcc ggcgcgaccc
     1021 tcaaccgcct gcgggagccg ctgctgcgga ggctcagcga gctcctggat caggcgcccg
     1081 agggccgggg ctggaggaga ctggcggagc tggcggggag tcgcgggcgc ctccgcctca
     1141 gttgcctaga cctggagcag tgttctctta aggtactgga gcctgaagga agccccagcc
     1201 tgtgtctgct gaagttaatg ggtgaaaaag gttgcacagt cacagaattg agtgatttcc
     1261 tgcaggctat ggaacacact gaagttcttc agcttctcag ccccccagga ataaagatta
     1321 ctgtaaaccc agagtcaaag gcagtcttgg ctggacagtt tgtgaaactg tgttgccggg
     1381 caactggaca tccttttgtt caatatcagt ggttcaaaat gaataaagag attccaaatg
     1441 gaaatacatc agagcttatt tttaatgcag tgcatgtaaa agatgcaggc ttttatgtct
     1501 gtcgagttaa taacaatttc acctttgaat tcagccagtg gtcacagctg gatgtttgcg
     1561 acatcccaga gagcttccag agaagtgttg atggcgtctc tgaatccaag ttgcaaatct
     1621 gtgttgaacc aacttcccaa aagctgatgc caggcagcac attggtttta cagtgtgttg
     1681 ctgttggaag ccctattcct cactaccagt ggttcaaaaa tgaattacca ttaacacatg
     1741 agaccaaaaa gctatacatg gtgccttatg tggatttgga acaccaagga acctactggt
     1801 gtcatgtata taatgatcga gacagtcaag atagcaagaa ggtagaaatc atcataggaa
     1861 gaacagatga ggcagtggag tgcactgaag atgaattaaa taatcttggt catcctgata
     1921 ataaagagca aacaactgac cagcctttgg cgaaggacaa ggttgccctt ttgataggaa
     1981 atatgaatta ccgggagcac cccaagctca aagctccttt ggtggatgtg tacgaattga
     2041 ctaacttact gagacagctg gacttcaaag tggtttcact gttggatctt actgaatatg
     2101 agatgcgtaa tgctgtggat gagtttttac tccttttaga caagggagta tatgggttat
     2161 tatattatgc aggacatggt tatgaaaatt ttgggaacag cttcatggtc cccgttgatg
     2221 ctccaaatcc atataggtct gaaaattgtc tgtgtgtaca aaatatactg aaattgatgc
     2281 aagaaaaaga aactggactt aatgtgttct tattggatat gtgtaggaaa agaaatgact
     2341 acgatgatac cattccaatc ttggatgcac taaaagtcac cgccaatatt gtgtttggat
     2401 atgccacgtg tcaaggagct gaagcttttg aaatccagca ttctggattg gcaaatggaa
     2461 tctttatgaa atttttaaaa gacagattat tagaagataa gaaaatcact gtgttactgg
     2521 atgaagttgc agaagatatg ggtaagtgtc accttaccaa aggcaaacag gctctagaga
     2581 ttcgaagtag tttatctgag aagagagcac ttactgatcc aatacaggga acagaatatt
     2641 ctgctgaatc tcttgtgcgg aatctacagt gggccaaggc tcatgaactt ccagaaagta
     2701 tgtgtcttaa gtttgactgt ggtgttcaga ttcaattagg atttgcagct gagttttcca
     2761 atgtcatgat catctataca agtatagttt acaaaccacc ggagataata atgtgtgatg
     2821 cctacgttac tgattttcca cttgatctag atattgatcc aaaagatgca aataaaggca
     2881 cgcctgaaga aactggcagc tacttggtat caaaggatct tcccaagcat tgcctctata
     2941 ccagactcag ttcactgcaa aaattaaagg aacatctagt cttcacagta tgtttatcat
     3001 atcagtactc aggattggaa gatactgtag aggacaagca ggaagtgaat gttgggaaac
     3061 ctctcattgc taaattagac atgcatcgag gtttgggaag gaagacttgc tttcaaactt
     3121 gtcttatgtc taatggtcct taccagagtt ctgcagccac ctcaggagga gcagggcatt
     3181 atcactcatt gcaagaccca ttccatggtg tttaccattc acatcctggt aatccaagta
     3241 atgttacacc agcagatagc tgtcattgca gccggactcc agatgcattt atttcaagtt
     3301 tcgctcacca tgcttcatgt cattttagta gaagtaatgt gccagtagag acaactgatg
     3361 aaataccatt tagtttctct gacaggctca gaatttctga aaaagggccc ttcgaacaaa
     3421 aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag
     3481 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     3541 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     3601 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     3661 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     3721 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     3781 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3841 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3901 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3961 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4021 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4081 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4141 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4201 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4261 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4321 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     4501 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     4561 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     4621 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     4681 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     4741 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     4801 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     4861 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     4921 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     4981 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     5041 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     5101 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     5161 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     5221 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     5281 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     5341 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     5401 cttctgagcg ggactctggg gttcgcgaaa tgaccgacca agcgacgccc aacctgccat
     5461 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5521 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5581 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5701 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5761 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5821 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5881 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5941 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6001 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6061 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6121 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6181 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6241 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6301 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6361 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6421 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6481 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6541 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6601 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6661 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6721 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6781 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6841 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6901 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6961 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7021 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7081 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7141 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7201 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7261 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7321 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7381 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7441 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7501 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7561 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7621 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7681 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7741 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7801 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7861 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//