LOCUS LT726919 6104 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCD-F-Bcl10, complete sequence.
ACCESSION LT726919
VERSION LT726919.1
KEYWORDS .
SOURCE Mammalian expression vector pCD-F-Bcl10
ORGANISM Mammalian expression vector pCD-F-Bcl10
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6104)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6104
/organism="Mammalian expression vector pCD-F-Bcl10"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5535."
/db_xref="taxon:1944904"
regulatory 235..818
/note="hCMV-IE promoter and enhancer"
/regulatory_class="promoter"
regulatory 863..879
/note="T7 promoter"
/regulatory_class="promoter"
misc_feature 923..946
/note="FLAG epitope"
CDS 968..1669
/transl_table=11
/note="hBcl-10"
/protein_id="SJL86939.1"
/translation="MEPTAPSLTEEDLTEVKKDALENLRVYLCEKIIAERHFDHLRAK
KILSREDTEEISCRTSSRKRAGKLLDYLQENPKGLDTLVESIRREKTQNFLIQKITDE
VLKLRNIKLEHLKGLKCSSCEPFPDGATNNLSRSNSDESNFSEKLRASTVMYHPEGES
STTPFFSTNSSLNLPVLEVGRTENTIFSSTTLPRPGDPGAPPLPPDLQLEEEGTCANS
SEMFLPLRSRTVSRQ"
regulatory 1701..1925
/note="BGH polyA"
/regulatory_class="terminator"
rep_origin 1971..2399
/note="f1 ori"
regulatory 2404..2685
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 2642..2717
/note="SV40 ori"
CDS 2809..3603
/transl_table=11
/note="Tn5 neomycin resistance"
/protein_id="SJL86940.1"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
regulatory 3777..3851
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3777..3851)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory complement(3990..4073)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4165..4567)
/note="pMB1 ori"
CDS complement(5108..5968)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86941.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1488 a 1558 c 1515 g 1543 t
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttacca tggattacaa ggacgatgac gataaggtac ctaaggatcc
961 tgccaccatg gagcccaccg caccgtccct caccgaggag gacctcactg aagtgaagaa
1021 ggacgcctta gaaaatttac gtgtatacct gtgtgagaaa atcatagctg agagacattt
1081 tgatcatcta cgtgcaaaaa aaatactcag tagagaagac actgaagaaa tttcttgtcg
1141 aacatcaagt agaaaaaggg ctggaaaatt gttagactac ttacaggaaa acccaaaagg
1201 tctggacacc cttgttgaat ctattcggcg agaaaaaaca cagaacttcc tgatacagaa
1261 gattacagat gaagtgctga aacttagaaa tataaaacta gaacatctga aaggactaaa
1321 atgtagcagt tgtgaacctt ttccagatgg agccacgaac aacctctcca gatcaaattc
1381 agatgagagt aatttctctg aaaaactgag ggcatccact gtcatgtacc atccagaagg
1441 agaatccagc acgacgccct ttttttctac taattcttct ctgaatttgc ctgttctaga
1501 agtaggcaga actgaaaata ccatcttctc ttcaactaca cttcccagac ctggggaccc
1561 aggggctcct cctttgccac cagatctaca gttagaagaa gaaggaactt gtgcaaactc
1621 tagtgagatg tttcttccct taagatcacg tactgtttca cgacaatgag ggcccgttta
1681 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
1741 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
1801 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
1861 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
1921 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
1981 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
2041 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
2101 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
2161 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
2221 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
2281 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
2341 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
2401 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
2461 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
2521 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
2581 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
2641 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
2701 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
2761 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
2821 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
2881 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
2941 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
3001 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
3061 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
3121 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
3181 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
3241 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
3301 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
3361 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
3421 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
3481 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
3541 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
3601 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
3661 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
3721 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
3781 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
3841 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
3901 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
3961 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
4021 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
4081 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
4141 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
4201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
4261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
4321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
4381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
4441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
4501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
4561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
4621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
4681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
4741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
4801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
4861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
4921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
4981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
5041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
5101 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
5161 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
5221 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
5281 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
5341 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
5401 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
5461 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
5521 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
5581 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
5641 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
5701 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
5761 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
5821 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
5881 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
5941 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
6001 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
6061 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
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