LOCUS LT726915 5627 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha-HS, complete sequence. ACCESSION LT726915 VERSION LT726915.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha-HS ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha-HS other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5627) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..5627 /organism="Mammalian expression vector pCAGGS-Flag-IKKalpha-HS" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5488." /db_xref="taxon:1944847" misc_feature 13..36 /note="FLAG epitope" CDS <43..825 /codon_start=1 /transl_table=11 /note="hIKKalpha-HS" /protein_id="SJL86931.1" /translation="SIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGY LEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMV KIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGK RQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDG ETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE" regulatory 913..1004 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 1005..1361 /note="3' FR of beta-globin" regulatory complement(1439..1521) /note="lac promoter" /regulatory_class="promoter" rep_origin 1663..1738 /note="SV40 ori" regulatory 1769..1843 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(1769..1843) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin complement(1958..2359) /note="pMB1 ori" CDS complement(2900..3760) /transl_table=11 /note="ampicillin" /protein_id="SJL86932.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 3900..4277 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 4283..5624 /note="AG promoter" /regulatory_class="promoter" misc_feature 4652..5576 /note="intron AG promoter" BASE COUNT 1262 a 1432 c 1547 g 1386 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccagcattca gcttgacttg 61 gagagataca gcgagcagat gacgtatggg atatcttcag aaaaaatgct aaaagcatgg 121 aaagaaatgg aagaaaaggc catccactat gctgaggttg gtgtcattgg atacctggag 181 gatcagatta tgtctttgca tgctgaaatc atggagctac agaagagccc ctatggaaga 241 cgtcagggag acttgatgga atctctggaa cagcgtgcca ttgatctata taagcagtta 301 aaacacagac cttcagatca ctcctacagt gacagcacag agatggtgaa aatcattgtg 361 cacactgtgc agagtcagga ccgtgtgctc aaggagctgt ttggtcattt gagcaagttg 421 ttgggctgta agcagaagat tattgatcta ctccctaagg tggaagtggc cctcagtaat 481 atcaaagaag ctgacaatac tgtcatgttc atgcagggaa aaaggcagaa agaaatatgg 541 catctcctta aaattgcctg tacacagagt tctgcccggt cccttgtagg atccagtcta 601 gaaggtgcag taacccctca gacatcagca tggctgcccc cgacttcagc agaacatgat 661 cattctctgt catgtgtggt aactcctcaa gatggggaga cttcagcaca aatgatagaa 721 gaaaatttga actgccttgg ccatttaagc actattattc atgaggcaaa tgaggaacag 781 ggcaatagta tgatgaatct tgattggagt tggttaacag aatgactcga ggaattcact 841 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac 901 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt 961 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat 1021 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat 1081 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt 1141 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 1201 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 1261 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 1321 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag 1381 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 1441 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa 1501 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag 1561 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc 1621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 1681 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 1741 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa 1801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 1861 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc 1921 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 1981 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 2041 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc 2101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 2161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 2221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 2281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 2341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 2401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 2461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 2521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 2581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 2641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 2701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 2761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 2881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 2941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 3001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 3061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 3121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 3181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 3241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 3301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 3361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 3421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 3481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 3541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 3601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 3661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 3721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 3841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg 3901 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 3961 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 4021 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 4081 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 4141 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 4201 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 4261 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc 4321 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat 4381 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg 4441 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc 4501 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg 4561 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc 4621 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 4681 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag 4741 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt 4801 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg 4861 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg 4921 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg 4981 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc 5041 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg 5101 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg 5161 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg 5221 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc 5281 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc 5341 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg 5401 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc 5461 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga 5521 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc 5581 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa //