LOCUS       LT726915                5627 bp    DNA     circular SYN 06-FEB-2017
DEFINITION  Mammalian expression vector pCAGGS-Flag-IKKalpha-HS, complete
            sequence.
ACCESSION   LT726915
VERSION     LT726915.1
KEYWORDS    .
SOURCE      Mammalian expression vector pCAGGS-Flag-IKKalpha-HS
  ORGANISM  Mammalian expression vector pCAGGS-Flag-IKKalpha-HS
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   De Schamphelaire W.
  JOURNAL   Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM.
REFERENCE   2
  AUTHORS   De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
            Roggeman Fonseca M., Vanhoucke M., Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished.
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Mammalian expression vector
                     pCAGGS-Flag-IKKalpha-HS"
                     /mol_type="other DNA"
                     /note="BCCM/LMBP Plasmid collection (Ghent
                     University,Belgium) accession number LMBP 5488."
                     /db_xref="taxon:1944847"
     misc_feature    13..36
                     /note="FLAG epitope"
     CDS             <43..825
                     /codon_start=1
                     /transl_table=11
                     /note="hIKKalpha-HS"
                     /protein_id="SJL86931.1"
                     /translation="SIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGY
                     LEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMV
                     KIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGK
                     RQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAWLPPTSAEHDHSLSCVVTPQDG
                     ETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDWSWLTE"
     regulatory      913..1004
                     /note="beta-globin polyA"
                     /regulatory_class="terminator"
     misc_feature    1005..1361
                     /note="3' FR of beta-globin"
     regulatory      complement(1439..1521)
                     /note="lac promoter"
                     /regulatory_class="promoter"
     rep_origin      1663..1738
                     /note="SV40 ori"
     regulatory      1769..1843
                     /note="SV40 polyA early"
                     /regulatory_class="terminator"
     regulatory      complement(1769..1843)
                     /note="SV40 polyA late"
                     /regulatory_class="terminator"
     rep_origin      complement(1958..2359)
                     /note="pMB1 ori"
     CDS             complement(2900..3760)
                     /transl_table=11
                     /note="ampicillin"
                     /protein_id="SJL86932.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3900..4277
                     /note="hCMV-IE enhancer"
                     /regulatory_class="promoter"
     regulatory      4283..5624
                     /note="AG promoter"
                     /regulatory_class="promoter"
     misc_feature    4652..5576
                     /note="intron AG promoter"
BASE COUNT         1262 a         1432 c         1547 g         1386 t
ORIGIN      
        1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccagcattca gcttgacttg
       61 gagagataca gcgagcagat gacgtatggg atatcttcag aaaaaatgct aaaagcatgg
      121 aaagaaatgg aagaaaaggc catccactat gctgaggttg gtgtcattgg atacctggag
      181 gatcagatta tgtctttgca tgctgaaatc atggagctac agaagagccc ctatggaaga
      241 cgtcagggag acttgatgga atctctggaa cagcgtgcca ttgatctata taagcagtta
      301 aaacacagac cttcagatca ctcctacagt gacagcacag agatggtgaa aatcattgtg
      361 cacactgtgc agagtcagga ccgtgtgctc aaggagctgt ttggtcattt gagcaagttg
      421 ttgggctgta agcagaagat tattgatcta ctccctaagg tggaagtggc cctcagtaat
      481 atcaaagaag ctgacaatac tgtcatgttc atgcagggaa aaaggcagaa agaaatatgg
      541 catctcctta aaattgcctg tacacagagt tctgcccggt cccttgtagg atccagtcta
      601 gaaggtgcag taacccctca gacatcagca tggctgcccc cgacttcagc agaacatgat
      661 cattctctgt catgtgtggt aactcctcaa gatggggaga cttcagcaca aatgatagaa
      721 gaaaatttga actgccttgg ccatttaagc actattattc atgaggcaaa tgaggaacag
      781 ggcaatagta tgatgaatct tgattggagt tggttaacag aatgactcga ggaattcact
      841 cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac
      901 aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt
      961 gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat
     1021 tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat
     1081 gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt
     1141 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat
     1201 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct
     1261 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga
     1321 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag
     1381 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     1441 acacaacata cgagccggaa gcataaagtg taaagcctgg gtgcctaatg agtgagctaa
     1501 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1561 cggatccgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     1621 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     1681 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     1741 ttggaggcct aggcttttgc aaaaagctaa cttgtttatt gcagcttata atggttacaa
     1801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1861 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgctgca ttaatgaatc
     1921 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1981 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     2041 atacggttat ccacagaatc agggataacg caggaaagaa catgtgagca aaaggccagc
     2101 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2161 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2221 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2281 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2341 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2401 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2461 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2521 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2581 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2641 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2701 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     2761 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     2821 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     2881 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     2941 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3001 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3061 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3121 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3181 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3241 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3301 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3361 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3421 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3481 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3541 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3601 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3661 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3721 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     3781 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     3841 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gggtcgatcg
     3901 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     3961 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     4021 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     4081 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     4141 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     4201 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     4261 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     4321 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     4381 gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg
     4441 cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
     4501 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
     4561 agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
     4621 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
     4681 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
     4741 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
     4801 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
     4861 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     4921 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     4981 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     5041 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     5101 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     5161 gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg
     5221 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     5281 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     5341 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     5401 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     5461 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     5521 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     5581 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa
//