LOCUS LT726910 7082 bp DNA circular SYN 06-FEB-2017 DEFINITION Mammalian expression vector pCAGGS-Flag-IKKalpha, complete sequence. ACCESSION LT726910 VERSION LT726910.1 KEYWORDS . SOURCE Mammalian expression vector pCAGGS-Flag-IKKalpha ORGANISM Mammalian expression vector pCAGGS-Flag-IKKalpha other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7082) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..7082 /organism="Mammalian expression vector pCAGGS-Flag-IKKalpha" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5483." /db_xref="taxon:1944843" misc_feature 13..36 /note="FLAG epitope" CDS 43..2280 /transl_table=11 /note="hIKKalpha" /protein_id="SJL86921.1" /translation="MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIK SCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNILIHDVPLLAMEYCSGGD LRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKII HKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGY RPFLHHLQPFTWHEKIKKKDPKCIFACEEMSGEVRFSSHLPQPNSLCSLVVEPMENWL QLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESL HSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKT VYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRA AMLSLLRYNANLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKML KAWKEMEEKAIHYAEVGVIGYLEDQIMSLHAEIMELQKSPYGRRQGDLMESLEQRAID LYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPK VEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGAVTPQTSAW LPPTSAEHDHSLSCVVTPQDGETSAQMIEENLNCLGHLSTIIHEANEEQGNSMMNLDW SWLTE" regulatory 2368..2459 /note="beta-globin polyA" /regulatory_class="terminator" misc_feature 2460..2816 /note="3' FR of beta-globin" regulatory complement(2894..2976) /note="lac promoter" /regulatory_class="promoter" rep_origin 3118..3193 /note="SV40 ori" regulatory complement(3224..3298) /note="SV40 polyA late" /regulatory_class="terminator" regulatory 3224..3298 /note="SV40 polyA early" /regulatory_class="terminator" rep_origin complement(3413..3814) /note="pMB1 ori" CDS complement(4355..5215) /transl_table=11 /note="ampicillin" /protein_id="SJL86922.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" regulatory 5355..5732 /note="hCMV-IE enhancer" /regulatory_class="promoter" regulatory 5738..7079 /note="AG promoter" /regulatory_class="promoter" misc_feature 6107..7031 /note="intron AG promoter" BASE COUNT 1705 a 1690 c 1886 g 1801 t ORIGIN 1 ttcctcgaga tggactacaa ggacgacgat gacaagggta ccatggagcg gcccccgggg 61 ctgcggccgg gcgcgggcgg gccctgggag atgcgggagc ggctgggcac cggcggcttc 121 gggaacgtct gtctgtacca gcatcgggaa cttgatctca aaatagcaat taagtcttgt 181 cgcctagagc taagtaccaa aaacagagaa cgatggtgcc atgaaatcca gattatgaag 241 aagttgaacc atgccaatgt tgtaaaggcc tgtgatgttc ctgaagaatt gaatattttg 301 attcatgatg tgcctcttct agcaatggaa tactgttctg gaggagatct ccgaaagctg 361 ctcaacaaac cagaaaattg ttgtggactt aaagaaagcc agatactttc tttactaagt 421 gatatagggt ctgggattcg atatttgcat gaaaacaaaa ttatacatcg agatctaaaa 481 cctgaaaaca tagttcttca ggatgttggt ggaaagataa tacataaaat aattgatctg 541 ggatatgcca aagatgttga tcaaggaagt ctgtgtacat cttttgtggg aacactgcag 601 tatctggccc cagagctctt tgagaataag ccttacacag ccactgttga ttattggagc 661 tttgggacca tggtatttga atgtattgct ggatataggc cttttttgca tcatctgcag 721 ccatttacct ggcatgagaa gattaagaag aaggatccaa agtgtatatt tgcatgtgaa 781 gagatgtcag gagaagttcg gtttagtagc catttacctc aaccaaatag cctttgtagt 841 ttagtagtag aacccatgga aaactggcta cagttgatgt tgaattggga ccctcagcag 901 agaggaggac ctgttgacct tactttgaag cagccaagat gttttgtatt aatggatcac 961 attttgaatt tgaagatagt acacatccta aatatgactt ctgcaaagat aatttctttt 1021 ctgttaccac ctgatgaaag tcttcattca ctacagtctc gtattgagcg tgaaactgga 1081 ataaatactg gttctcaaga acttctttca gagacaggaa tttctctgga tcctcggaaa 1141 ccagcctctc aatgtgttct agatggagtt agaggctgtg atagctatat ggtttatttg 1201 tttgataaaa gtaaaactgt atatgaaggg ccatttgctt ccagaagttt atctgattgt 1261 gtaaattata ttgtacagga cagcaaaata cagcttccaa ttatacagct gcgtaaagtg 1321 tgggctgaag cagtgcacta tgtgtctgga ctaaaagaag actatagcag gctctttcag 1381 ggacaaaggg cagcaatgtt aagtcttctt agatataatg ctaacttaac aaaaatgaag 1441 aacactttga tctcagcatc acaacaactg aaagctaaat tggagttttt tcacaaaagc 1501 attcagcttg acttggagag atacagcgag cagatgacgt atgggatatc ttcagaaaaa 1561 atgctaaaag catggaaaga aatggaagaa aaggccatcc actatgctga ggttggtgtc 1621 attggatacc tggaggatca gattatgtct ttgcatgctg aaatcatgga gctacagaag 1681 agcccctatg gaagacgtca gggagacttg atggaatctc tggaacagcg tgccattgat 1741 ctatataagc agttaaaaca cagaccttca gatcactcct acagtgacag cacagagatg 1801 gtgaaaatca ttgtgcacac tgtgcagagt caggaccgtg tgctcaagga gctgtttggt 1861 catttgagca agttgttggg ctgtaagcag aagattattg atctactccc taaggtggaa 1921 gtggccctca gtaatatcaa agaagctgac aatactgtca tgttcatgca gggaaaaagg 1981 cagaaagaaa tatggcatct ccttaaaatt gcctgtacac agagttctgc ccggtccctt 2041 gtaggatcca gtctagaagg tgcagtaacc cctcagacat cagcatggct gcccccgact 2101 tcagcagaac atgatcattc tctgtcatgt gtggtaactc ctcaagatgg ggagacttca 2161 gcacaaatga tagaagaaaa tttgaactgc cttggccatt taagcactat tattcatgag 2221 gcaaatgagg aacagggcaa tagtatgatg aatcttgatt ggagttggtt aacagaatga 2281 ctcgaggaat tcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc 2341 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac 2401 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca 2461 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat 2521 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct 2581 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc 2641 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt 2701 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt 2761 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc 2821 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 2881 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctgggtgcc 2941 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga 3001 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa 3061 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 3121 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 3181 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc 3241 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 3301 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg 3361 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc 3421 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 3481 cactcaaagg cggtaatacg gttatccaca gaatcaggga taacgcagga aagaacatgt 3541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 3721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 3781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 3841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 3901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 3961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 4141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 4201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 4801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 4861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 4921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 4981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 5041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 5101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 5161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 5221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5341 cacctgggtc gatcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc 5401 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc 5461 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt 5521 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca 5581 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg 5641 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca 5701 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt 5761 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt 5821 attttgtgca gcgatggggg cggggggggg gggggggccc cccccaggcg gggcggggcg 5881 gggcgagggg cggggcgggg cgaggcggaa aggtgcggcg gcagccaatc agagcggcgc 5941 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag 6001 cgcgcggcgg gcgggagtcg ttgcgcgctg ccttcccccc gtgccccgct ccgccgccgc 6061 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga 6121 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct 6181 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc 6241 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg 6301 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg 6361 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg 6421 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca 6481 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct 6541 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt 6601 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaagggg cgcggcggcc 6661 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat 6721 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga 6781 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg 6841 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc 6901 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg 6961 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct 7021 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag 7081 aa //