LOCUS LT726909 8251 bp DNA circular SYN 06-FEB-2017 DEFINITION Vector pCal-n-MK, complete sequence. ACCESSION LT726909 VERSION LT726909.1 KEYWORDS . SOURCE Vector pCal-n-MK ORGANISM Vector pCal-n-MK other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8251) AUTHORS De Schamphelaire W. JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K., Roggeman Fonseca M., Vanhoucke M., Beyaert R. TITLE BCCM/LMBP Plasmid collection JOURNAL Unpublished. FEATURES Location/Qualifiers source 1..8251 /organism="Vector pCal-n-MK" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University,Belgium) accession number LMBP 5466." /db_xref="taxon:1945272" regulatory complement(213..260) /note="T7 terminator" /regulatory_class="terminator" CDS complement(<331..2802) /transl_table=11 /note="hMLT" /protein_id="SJL86917.1" /translation="MALLGDPLQALPPSAAPTGPLLAPPAGATLNRLREPLLRRLSEL LDQAPEGRGWRRLAELAGSRGRLRLSCLDLEQCSLKVLEPEGSPSLCLLKLMGEKGCT VTELSDFLQAMEHTEVLQLLSPPGIKITVNPESKAVLAGQFVKLCCRATGHPFVQYQW FKMNKEIPNGNTSELIFNAVHVKDAGFYVCRVNNNFTFEFSQWSQLDVCDIPESFQRS VDGVSESKLQICVEPTSQKLMPGSTLVLQCVAVGSPIPHYQWFKNELPLTHETKKLYM VPYVDLEHQGTYWCHVYNDRDSQDSKKVEIIIGRTDEAVECTEDELNNLGHPDNKEQT TDQPLAKDKVALLIGNMNYREHPKLKAPLVDVYELTNLLRQLDFKVVSLLDLTEYEMR NAVDEFLLLLDKGVYGLLYYAGHGYENFGNSFMVPVDAPNPYRSENCLCVQNILKLMQ EKETGLNVFLLDMCRKRNDYDDTIPILDALKVTANIVFGYATCQGAEAFEIQHSGLAN GIFMKFLKDRLLEDKKITVLLDEVAEDMGKCHLTKGKQALEIRSSLSEKRALTDPIQG TEYSAESLVRNLQWAKAHELPESMCLKFDCGVQIQLGFAAEFSNVMIIYTSIVYKPPE IIMCDAYVTDFPLDLDIDPKDANKGTPEETGSYLVSKDLPKHCLYTRLSSLQKLKEHL VFTVCLSYQYSGLEDTVEDKQEVNVGKPLIAKLDMHRGLGRKTCFQTCLMSNGPYQSS AATSGGAGHYHSLQDPFHGVYHSHPGNPSNVTPADSCHCSRTPDAFISSFAHHASCHF SRSNVPVETTDEIPFSFSDRLRISEK" misc_feature complement(853..855) /note="PCR artefact" misc_feature complement(1315..1317) /note="stability creator" misc_feature complement(2797..2799) /note="Kozak" misc_feature complement(2836..2853) /note="thrombin recognition site" misc_feature complement(2854..2931) /note="calmodulin-binding peptide tag" misc_feature complement(2935..2979) /note="5' UTR of T7 gene 10, incl. RBS" regulatory complement(2981..3022) /note="T7 promoter with lac operator" /regulatory_class="promoter" regulatory 3331..3408 /note="lacI promoter" /regulatory_class="promoter" CDS 3409..4491 /transl_table=11 /note="lacI" /protein_id="SJL86918.1" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" CDS 5803..5994 /transl_table=11 /note="rop" /protein_id="SJL86919.1" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH ADELYRSCLARFGDDGENL" rep_origin complement(6240..6642) /note="pMB1 ori" CDS complement(7183..8043) /transl_table=11 /note="ampicillin resistance" /protein_id="SJL86920.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1985 a 2155 c 2031 g 2080 t ORIGIN 1 ttgtcatgtt tgacagctta tcttcgatta gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 301 cgggctttgt tagcagccgg atctaagctc tttttcagaa attctgagcc tgtcagagaa 361 actaaatggt atttcatcag ttgtctctac tggcacatta cttctactaa aatgacatga 421 agcatggtga gcgaaacttg aaataaatgc atctggagtc cggctgcaat gacagctatc 481 tgctggtgta acattacttg gattaccagg atgtgaatgg taaacaccat ggaatgggtc 541 ttgcaatgag tgataatgcc ctgctcctcc tgaggtggct gcagaactct ggtaaggacc 601 attagacata agacaagttt gaaagcaagt cttccttccc aaacctcgat gcatgtctaa 661 tttagcaatg agaggtttcc caacattcac ttcctgcttg tcctctacag tatcttccaa 721 tcctgagtac tgatatgata aacatactgt gaagactaga tgttccttta atttttgcag 781 tgaactgagt ctggtataga ggcaatgctt gggaagatcc tttgatacca agtagctgcc 841 agtttcttca ggcgtgcctt tatttgcatc ttttggatca atatctagat caagtggaaa 901 atcagtaacg taggcatcac acattattat ctccggtggt ttgtaaacta tacttgtata 961 gatgatcatg acattggaaa actcagctgc aaatcctaat tgaatctgaa caccacagtc 1021 aaacttaaga cacatacttt ctggaagttc atgagccttg gcccactgta gattccgcac 1081 aagagattca gcagaatatt ctgttccctg tattggatca gtaagtgctc tcttctcaga 1141 taaactactt cgaatctcta gagcctgttt gcctttggta aggtgacact tacccatatc 1201 ttctgcaact tcatccagta acacagtgat tttcttatct tctaataatc tgtcttttaa 1261 aaatttcata aagattccat ttgccaatcc agaatgctgg atttcaaaag cttcagctcc 1321 ttgacacgtg gcatatccaa acacaatatt ggcggtgact tttagtgcat ccaagattgg 1381 aatggtatca tcgtagtcat ttcttttcct acacatatcc aataagaaca cattaagtcc 1441 agtttctttt tcttgcatca atttcagtat attttgtaca cacagacaat tttcagacct 1501 atatggattt ggagcatcaa cggggaccat gaagctgttc ccaaaatttt cataaccatg 1561 tcctgcataa tataataacc catatactcc cttgtctaaa aggagtaaaa actcatccac 1621 agcattacgc atctcatatt cagtaagatc caacagtgaa accactttga agtccagctg 1681 tctcagtaag ttagtcaatt cgtacacatc caccaaagga gctttgagct tggggtgctc 1741 ccggtaattc atatttccta tcaaaagggc aaccttgtcc ttcgccaaag gctggtcagt 1801 tgtttgctct ttattatcag gatgaccaag attatttaat tcatcttcag tgcactccac 1861 tgcctcatct gttcttccta tgatgatttc taccttcttg ctatcttgac tgtctcgatc 1921 attatataca tgacaccagt aggttccttg gtgttccaaa tccacataag gcaccatgta 1981 tagctttttg gtctcatgtg ttaatggtaa ttcatttttg aaccactggt agtgaggaat 2041 agggcttcca acagcaacac actgtaaaac caatgtgctg cctggcatca gcttttggga 2101 agttggttca acacagattt gcaacttgga ttcagagacg ccatcaacac ttctctggaa 2161 gctctctggg atgtcgcaaa catccagctg tgaccactgg ctgaattcaa aggtgaaatt 2221 gttattaact cgacagacat aaaagcctgc atcttttaca tgcactgcat taaaaataag 2281 ctctgatgta tttccatttg gaatctcttt attcattttg aaccactgat attgaacaaa 2341 aggatgtcca gttgcccggc aacacagttt cacaaactgt ccagccaaga ctgcctttga 2401 ctctgggttt acagtaatct ttattcctgg ggggctgaga agctgaagaa cttcagtgtg 2461 ttccatagcc tgcaggaaat cactcaattc tgtgactgtg caaccttttt cacccattaa 2521 cttcagcaga cacaggctgg ggcttccttc aggctccagt accttaagag aacactgctc 2581 caggtctagg caactgaggc ggaggcgccc gcgactcccc gccagctccg ccagtctcct 2641 ccagccccgg ccctcgggcg cctgatccag gagctcgctg agcctccgca gcagcggctc 2701 ccgcaggcgg ttgagggtcg cgccggccgg aggggcgagc agcggccccg tgggggcggc 2761 cgagggcggc agggcctgta gcgggtcccc caacagcgcc atggtggcag gatccatgga 2821 gtctagaatt cccggggatc cacgcggaac cagaagtgcc ccggaggatg agattttctt 2881 aaagcggttg gctgctgaga cggctatgaa attctttttc catcgtcgct tcatatgtat 2941 atctccttct taaagttaaa caaaattatt tctagagggg aattgttatc cgctcacaat 3001 tcccctatag tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac 3061 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca 3121 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg 3181 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat 3241 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg 3301 agtcgcataa gggagagcgt cgagatcccg gacaccatcg aatggcgcaa aacctttcgc 3361 ggtatggcat gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta 3421 acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg 3481 aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag 3541 ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt 3601 ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa 3661 tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc 3721 gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt 3781 aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg 3841 gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa 3901 gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg 3961 ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat 4021 ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc 4081 ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt 4141 gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt 4201 ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg 4261 ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg 4321 ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg 4381 aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 4441 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 4501 aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc ccttgagagc 4561 cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg ccgcacttat 4621 gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct gggtcatttt 4681 cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg cggtattcgg 4741 aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac gtttcggcga 4801 gaagcaggcc attatcgccg gcatggcggc cgacgcgctg ggctacgtct tgctggcgtt 4861 cgcgacgcga ggctggatgg ccttccccat tatgattctt ctcgcttccg gcggcatcgg 4921 gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat gacgaccatc agggacagct 4981 tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc actggaccgc tgatcgtcac 5041 ggcgatttat gccgcctcgg cgagcacatg gaacgggttg gcatggattg taggcgccgc 5101 cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca tggagccggg ccacctcgac 5161 ctgaatggaa gccggcggca cctcgctaac ggattcacca ctccaagaat tggagccaat 5221 caattcttgc ggagaactgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg 5281 tccgccatct ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg 5341 gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact 5401 ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg 5461 tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa 5521 acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg 5581 ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt 5641 tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac 5701 cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc 5761 attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa acaggaaaaa 5821 accgccctta acatggcccg ctttatcaga agccagacat taacgcttct ggagaaactc 5881 aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga ccacgctgat 5941 gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg 6001 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt 6061 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc 6121 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 6181 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 6241 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6301 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6361 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6421 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 6481 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 6541 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 6601 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 6661 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 6721 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 6781 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 6841 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 6901 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 6961 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7021 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7081 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7141 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7201 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7261 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7321 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7381 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7441 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 7501 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 7561 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 7621 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 7681 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 7741 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 7801 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 7861 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 7921 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 7981 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8041 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8101 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8161 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8221 gcgtatcacg aggccctttc gtcttcaaga a //