LOCUS LT726880 4977 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mABIN-1(469-524), complete
sequence.
ACCESSION LT726880
VERSION LT726880.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mABIN-1(469-524)
ORGANISM Mammalian expression vector pCAGGS-E-mABIN-1(469-524)
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4977)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..4977
/organism="Mammalian expression vector
pCAGGS-E-mABIN-1(469-524)"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 5376."
/db_xref="taxon:1944798"
misc_feature 16..54
/note="E-tag"
CDS 64..234
/transl_table=11
/note="mABIN-1(469-524)"
/protein_id="SJL86756.1"
/translation="MTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQA
QVTLTNAQLKTL"
regulatory 267..358
/note="beta-globin polyA"
/regulatory_class="terminator"
misc_feature 359..715
/note="3' FR of beta-globin"
regulatory complement(793..875)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1017..1092
/note="SV40 ori"
regulatory complement(1123..1197)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1123..1197
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(1312..1713)
/note="pMB1 ori"
CDS complement(2254..3114)
/transl_table=11
/note="ampicillin"
/protein_id="SJL86757.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3250..3627
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 3633..4974
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4002..4926
/note="intron AG promoter"
BASE COUNT 1061 a 1317 c 1388 g 1211 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcagtgacac agaatgagtt gctgaaacag caggtaaaga tctttgaaga ggacttccag
121 agggaacgga gtgaccgtga acgcatgaat gaagagaagg aggagctgaa gaagcaagta
181 gagaagctgc aggcccaggt caccctgact aatgcccagc tcaaaactct ctgaccccca
241 atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat tatggggaca
301 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
361 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
421 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
481 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
541 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
601 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
661 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
721 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
781 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctgggtgcct
841 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
901 acctgtcgtg ccagcggatc cgcatctcaa ttagtcagca accatagtcc cgcccctaac
961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
1021 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
1081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct
1141 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
1201 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatccgc
1261 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
1321 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
1381 actcaaaggc ggtaatacgg ttatccacag aatcagggat aacgcaggaa agaacatgtg
1441 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
1501 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
1561 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
1621 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
1681 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
1741 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
1801 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
1861 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
1921 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
1981 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
2041 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
2101 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
2161 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
2221 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
2281 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
2341 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
2401 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
2461 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
2521 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
2581 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
2641 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
2701 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
2761 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
2821 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
2881 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
2941 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
3001 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
3061 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
3121 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
3181 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
3241 acctgggtcg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
3301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
3361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
3421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
3481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
3541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
3601 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
3661 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
3721 gtgcagcgat gggggcgggg gggggggggg ggcccccccc aggcggggcg gggcggggcg
3781 aggggcgggg cggggcgagg cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc
3841 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
3901 ggcgggcggg agtcgttgcg cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc
3961 gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc
4021 cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc
4081 tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg
4141 gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct
4201 gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg
4261 cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc
4321 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc
4381 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta
4441 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
4501 gcggggcggg gccgcctcgg gccggggagg gctcggggga aggggcgcgg cggcccccgg
4561 agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc
4621 gagagggcgc agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc
4681 cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg
4741 ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg
4801 ggctgtccgc ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc
4861 tggcgtgtga ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc
4921 ctacagctcc tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaa
//