LOCUS LT726871 6245 bp DNA circular SYN 06-FEB-2017
DEFINITION Shuttle vector pAM1, complete sequence.
ACCESSION LT726871
VERSION LT726871.1
KEYWORDS .
SOURCE Shuttle vector pAM1
ORGANISM Shuttle vector pAM1
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 6245)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..6245
/organism="Shuttle vector pAM1"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 8058."
/db_xref="taxon:1945223"
CDS complement(499..1233)
/transl_table=11
/note="erythromycin resistance"
/protein_id="SJL86727.1"
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI
PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR
EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
AGIDDLNNISFEQFLSLFNSYKLFNK"
CDS 1698..1982
/transl_table=11
/note="copG-like"
/protein_id="SJL86728.1"
/translation="MSDFSERDRELLAMFGMGEDQVRDDVERMESETAGHGITGPVHY
ALHMLPEHGEEMVSMTVKLPKSQLEKVTETARRYHISRSEYVRRQLAGAV"
CDS complement(2174..2464)
/transl_table=11
/note="orfX-like"
/protein_id="SJL86729.1"
/translation="MTIQTIRKKRPLPAKELAAMYDVSVRTIQRWASQTRKDWIDEQA
TLRESIRAYHDDEGHTWPQTAEHFGMSQDAVRSRCYRARKERAAEAKAARPE"
CDS complement(2461..3408)
/transl_table=11
/note="repB"
/protein_id="SJL86730.1"
/translation="MSDEYSQPTLELSRTFEGWWLPRRPLCCDDDYSQLVRRSRTDAL
RCKHIEANPSALVNTIVVDIDDANAKAMALWGHRGMLPNWIAENPANGHAHAGWVLTY
PVPRTDMARLKPLKLLHAVTEGLRRSVDGDEGYSGLLMKNPLSDAWDSDLCREDTYDL
PDLVAALEEHGDMPPKSWTRTKRAREVGVGRNCTLFDEARTLAYRQVRRLPDRTPASS
DLLREYVRRTCHEINASFPDPLPVREVNDTAKSIHKWITTRSRMWRDGAVANAATFVA
IQSARGRKGGRGNKRDKQGNVQNAFKQKAELFGKEMMGQ"
misc_feature complement(4029..4066)
/note="5' UTR of lacZ, incl. RBS"
regulatory complement(4067..4150)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin complement(4242..4644)
/note="pMB1 ori"
CDS complement(5185..6045)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86731.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
BASE COUNT 1447 a 1604 c 1645 g 1543 t
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccnnngtc
421 gacaaaagac ataatcgatt cacaaaaaat aggcacacga aaaacaagtt aagggatgca
481 gtttatgcat cccttaactt acttattaaa taatttatag ctattgaaaa gagataagaa
541 ttgttcaaag ctaatattgt ttaaatcgtc aattcctgca tgttttaagg aattgttaaa
601 ttgatttttt gtaaatattt tcttgtattc tttgttaacc catttcataa cgaaataatt
661 atacttttgt ttatctttgt gtgatattct tgattttttt ctacttaatc tgataagtga
721 gctattcact ttaggtttag gatgaaaata ttctcttgga accatactta atatagaaat
781 atcaacttct gccattaaaa gtaatgccaa tgagcgtttt gtatttaata atcttttagc
841 aaacccgtat tccacgatta aataaatctc attagctata ctatcaaaaa caattttgcg
901 tattatatcc gtacttatgt tataaggtat attaccatat attttatagg attggttttt
961 aggaaattta aactgcaata tatccttgtt taaaacttgg aaattatcgt gatcaacaag
1021 tttattttct gtagttttgc ataatttatg gtctatttca atggcagtta cgaaattaca
1081 cctctttact aattcaaggg taaaatggcc ttttcctgag ccgatttcaa agatattatc
1141 atgttcattt aatcttatat ttgtcattat tttatctata ttatgttttg aagtaataaa
1201 gttttgactg tgttttatat ttttctcgtt cattataacc ctctttaatt tggttatatg
1261 aattttgctt attaacgatt cattataacc acttattttt tgtttggttg ataatgaact
1321 gtgctgatta caaaaatact aaaaatgccc atattttttc ctccttataa aattagtata
1381 attatagcac gagctctgat aaatatgaac atgatgagtg atcgttaacn nngggggatc
1441 tacgtgctgc cgcgcgtgca tgagcggcat cccgagctgt ccgtggagga cgtgcttgcg
1501 gcgttcagga gcgtcatggt ggacgcccgc agggacaacg gcacgtgggt ctgcatcggc
1561 ctcgacgggc gcggcaggga cgtggagatg gtctacgagc agcgcggcga ccgcgcgctc
1621 atctaccacg ccatgacgcc gccgacgaag aagacccgaa gcgagatagc ccgcctgcgc
1681 gggggaagga gcgaccgatg agcgatttca gcgaacgcga cagggagctg ttggcgatgt
1741 tcggcatggg cgaggatcag gtcagggacg acgtggagcg catggagtcc gagaccgcgg
1801 gccacggcat aaccggaccc gtccactacg cgctgcacat gctccccgag cacggcgagg
1861 agatggtcag catgaccgtc aagctgccca agagtcagct ggagaaggtc acggagaccg
1921 ccaggcgcta ccacatcagc cgcagcgagt acgtgcgccg gcagctcgcc ggagccgtct
1981 gaccgccgcc gcgcgaagac ggaacacccg tgtgccatca ttcgtggcac acgggtgttc
2041 ccataggtga caccgcggtg tcaccatgcc gcgcgagcgg tctggaggcg cagccgacca
2101 tggggtacgg gtcttcccgg caagccgatt cgcggtgcga atcgagtgaa cgtatatacg
2161 ttcacgtcac ttgctactcc ggccttgcgg cttttgcctc ggccgcacgc tccttgcgcg
2221 cgcggtagca gcggctacgc accgcgtctt ggctcatgcc gaagtgctcg gcggtctgcg
2281 gccacgtatg gccctcgtca tcgtggtagg cacggatgga ttcgcgcaac gttgcctgtt
2341 cgtctatcca gtccttacgg gtttgtgaag cccatcgctg aatagtgcgc acggagacgt
2401 cgtacattgc cgccagctcc ttggcgggaa gcggacgctt cttgcgaatc gtctgaatcg
2461 tcattgcccc atcatctcct tgccgaacag ctctgccttt tgcttgaagg cattttgaac
2521 gttcccttgc ttgtcgcgtt tgtttccgcg accgcctttg cgtccgcgcg cggattggat
2581 ggcgacgaac gtggcggcgt tggcgacggc accgtccctc cacatgcggc ttcgcgtggt
2641 gatccacttg tggatgctct tggcggtgtc gttgacctcg cgcacgggca gcgggtcggg
2701 gaacgaggcg ttgatttcgt ggcaggtgcg gcgcacgtac tcgcgcagca ggtcggagga
2761 ggcgggcgtg cggtcgggca gtcggcgcac ctgacggtag gcgagggtgc gggcctcgtc
2821 gaacaaggtg cagttgcggc ccacgccgac ctcgcgggcg cgtttggtgc gcgtccagct
2881 cttgggcggc atgtccccgt gctcttcgag cgcggccacg aggtcgggca ggtcgtaggt
2941 gtcctcgcgg cacaggtcgc tgtcccacgc gtcgctcagc gggttcttca tcagcaggcc
3001 ggaatagccc tcgtcgccgt ccacgctgcg gcgcagcccc tcggtgacgg cgtgcaggag
3061 cttcagcggt ttgagccggg ccatgtcggt gcggggcacg gggtaggtca gcacccagcc
3121 cgcgtgagcg tgcccgttgg ccgggttctc cgcgatccag ttcggcagca tcccacggtg
3181 cccccacagg gccatcgcct tggcgttcgc gtcgtcgatg tccaccacga tcgtgttcac
3241 cagcgccgag ggattcgcct cgatgtgctt gcatctgagc gcgtcggtgc ggctccggcg
3301 caccagctgg ctgtagtcgt cgtcgcagca cagcggacgc cggggcagcc accagccctc
3361 gaacgtgcgc gacagctcaa gcgtcggctg cgaatactcg tcagacatgg gcactagtgt
3421 acgtcgtact tgaccactgg tcaagttcgc ctaaaaccaa gtcagatatt tcagcccagc
3481 aaagcatggg ctgctccttc ccagagttac ccaaaactgt cgaaatctgg ccaaaaacac
3541 cccaaaccga acgatcatcc accctcatgc gcttcctatg cgctcagatg gatatatcca
3601 tggataccca catggacacg tctatggaca cccacatgga tatgtccatg gacatccaca
3661 tggagatgtc catcaaatcg aaggtaagca agctagacgg ttcatgtaca tgaaccgaac
3721 gcagcacctc ctgcgccctt cggcgcagga gcaacaaccc gaggccgagg aaaaagccgc
3781 aaggccggag tagcaagcga cgtgattgta gctacaatca ctcgattcgc ggtgcgaatc
3841 ggcttgccgg gaagacccgt accccatggt cggcggagcc tccagaccgc tcgcgcggct
3901 tgtgccacaa cggtgttcca cttgtgtgac cgtggtgtta ctctggtatc aactttgctt
3961 ggaggtgctg tttggagatc ctctagagtc gacctgcagg catgcaagct tggcgtaatc
4021 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
4081 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
4141 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
4201 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
4261 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
4321 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
4381 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
4441 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
4501 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
4561 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
4621 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
4681 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
4741 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
4801 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
4861 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
4921 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
4981 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
5041 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
5101 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
5161 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
5221 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
5281 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
5341 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
5401 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
5461 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
5521 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
5581 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
5641 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
5701 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
5761 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
5821 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
5881 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
5941 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
6001 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
6061 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
6121 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
6181 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
6241 tcgtc
//