LOCUS LT726851 5770 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-hbeclin-1-C, complete
sequence.
ACCESSION LT726851
VERSION LT726851.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-hbeclin-1-C
ORGANISM Mammalian expression vector pCAGGS-E-hbeclin-1-C
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5770)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5770
/organism="Mammalian expression vector
pCAGGS-E-hbeclin-1-C"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 7286."
/db_xref="taxon:1944773"
misc_feature 16..54
/note="E-tag"
CDS 64..972
/transl_table=11
/note="hBECN1-C fragment"
/protein_id="SJL86674.1"
/translation="MTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEE
RLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSV
ENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWG
QTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFD
HAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQ
WTKALKFMLTNLKWGLAWVSSQFYNK"
regulatory 1060..1151
/note="3' UTR of rabbit beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1152..1508
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1586..1668)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1810..1885
/note="SV40 ori"
regulatory complement(1916..1990)
/note="SV40 polyA late"
/regulatory_class="terminator"
regulatory 1916..1990
/note="SV40 polyA early"
/regulatory_class="terminator"
rep_origin complement(2105..2506)
/note="pMB1 ori"
CDS complement(3047..3907)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86675.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4043..4420
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4426..5767
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4795..5719
/note="intron AG promoter"
BASE COUNT 1278 a 1468 c 1615 g 1409 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcaatgactc agctcaacgt cactgaaaat gagtgtcaga actacaaacg ctgtttggag
121 atcttagagc aaatgaatga ggatgacagt gaacagttac agatggagct aaaggagctg
181 gcactagagg aggagaggct gatccaggag ctggaagacg tggaaaagaa ccgcaagata
241 gtggcagaaa atctcgagaa ggtccaggct gaggctgaga gactggatca ggaggaagct
301 cagtatcaga gagaatacag tgaatttaaa cgacagcagc tggagctgga tgatgagctg
361 aagagtgttg aaaaccagat gcgttatgcc cagacgcagc tggataagct gaagaaaacc
421 aacgtcttta atgcaacctt ccacatctgg cacagtggac agtttggcac aatcaataac
481 ttcaggctgg gtcgcctgcc cagtgttccc gtggaatgga atgagattaa tgctgcttgg
541 ggccagactg tgttgctgct ccatgctctg gccaataaga tgggtctgaa atttcagaga
601 taccgacttg ttccttacgg aaaccattca tatctggagt ctctgacaga caaatctaag
661 gagctgccgt tatactgttc tggggggttg cggtttttct gggacaacaa gtttgaccat
721 gcaatggtgg ctttcctgga ctgtgtgcag cagttcaaag aagaggttga gaaaggcgag
781 actcgttttt gtcttcccta caggatggat gtggagaaag gcaagattga agacacagga
841 ggcagtggcg gctcctattc catcaaaacc cagtttaact ctgaggagca gtggacaaaa
901 gctctcaagt tcatgctgac gaatcttaag tggggtcttg cttgggtgtc ctcacaattt
961 tataacaaat gagtcgagga attcactcct caggtgcagg ctgcctatca gaaggtggtg
1021 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa
1081 aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt
1141 attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg
1201 agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc
1261 catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag
1321 ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta gatttttttt
1381 atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta
1441 ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta
1501 tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt
1561 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
1621 agcctgggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
1681 ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag
1741 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
1801 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
1861 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
1921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
1981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
2041 tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
2101 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
2161 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg gataacgcag
2221 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
2281 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
2341 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
2401 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
2461 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
2521 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
2581 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
2641 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
2701 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
2761 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
2821 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
2881 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
2941 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
3001 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
3061 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
3121 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
3181 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
3241 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
3301 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
3361 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
3421 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
3481 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
3541 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
3601 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
3661 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
3721 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
3781 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
3841 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
3901 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
3961 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
4021 cccgaaaagt gccacctggg tcgacattga ttattgacta gttattaata gtaatcaatt
4081 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
4141 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
4201 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
4261 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
4321 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
4381 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
4441 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
4501 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggggccccc cccaggcggg
4561 gcggggcggg gcgaggggcg gggcggggcg aggcggaaag gtgcggcggc agccaatcag
4621 agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc ggcggcggcg gccctataaa
4681 aagcgaagcg cgcggcgggc gggagtcgtt gcgcgctgcc ttccccccgt gccccgctcc
4741 gccgccgcct cgcgccgccc gccccggctc tgactgaccg cgttactccc acaggtgagc
4801 gggcgggacg gcccttctcc tccgggctgt aattagcgct tggtttaatg acggcttgtt
4861 tcttttctgt ggctgcgtga aagccttgag gggctccggg agggcccttt gtgcgggggg
4921 agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg
4981 ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg
5041 cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac
5101 aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc
5161 gggctgcaac cccccctgca cccccctccc cgagttgctg agcacggccc ggcttcgggt
5221 gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag
5281 gtgggggtgc cgggcggggc ggggccgcct cgggccgggg agggctcggg ggaaggggcg
5341 cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc attgcctttt
5401 atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg cggagccgaa
5461 atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg
5521 caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc cccttctccc
5581 tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac ggggcagggc
5641 ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc atgttcatgc
5701 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
5761 tggcaaagaa
//