LOCUS LT726837 5666 bp DNA circular SYN 06-FEB-2017
DEFINITION Mammalian expression vector pCAGGS-E-mMyD88, complete sequence.
ACCESSION LT726837
VERSION LT726837.1
KEYWORDS .
SOURCE Mammalian expression vector pCAGGS-E-mMyD88
ORGANISM Mammalian expression vector pCAGGS-E-mMyD88
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 5666)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (02-FEB-2017) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Schamphelaire W., Olbrechts A., Meert J., Verhelst K.,
Roggeman Fonseca M., Vanhoucke M., Beyaert R.
TITLE BCCM/LMBP Plasmid collection
JOURNAL Unpublished.
FEATURES Location/Qualifiers
source 1..5666
/organism="Mammalian expression vector pCAGGS-E-mMyD88"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent
University,Belgium) accession number LMBP 6654."
/db_xref="taxon:1944812"
misc_feature 16..54
/note="E-tag"
CDS 64..954
/transl_table=11
/note="mMyd88"
/protein_id="SJL86639.1"
/translation="MSAGDPRVGSGSLDSFMFSIPLVALNVGVRRRLSLFLNPRTPVA
ADWTLLPEEMGFEYLEIRELETRPDPTRSLLDAWQGRSGASVGRLLELLALLDREDIL
KELKSRIEEDCQKYLGKQQNQESEKPLQVARVESSVPQTKELGGITTLDDPLGQTPEL
FDAFICYCPNDIEFVQEMIRQLEQTDYRLKLCVSDRDVLPGTCVWSIASELIEKRCRR
MVVVVSDDYLQSKECDFQTKFALSLSPGVQQKRLIPIKYKAMKKDFPSILRFITICDY
TNPCTKSWFWTRLAKALSLP"
regulatory 956..1047
/note="3' UTR of beta-globin, incl. polyA"
/regulatory_class="terminator"
misc_feature 1048..1404
/note="3' flanking region of rabbit beta-globin"
regulatory complement(1482..1564)
/note="lac promoter"
/regulatory_class="promoter"
rep_origin 1706..1781
/note="SV40 ori"
regulatory 1812..1886
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(1812..1886)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin complement(2001..2402)
/note="pMB1 ori"
CDS complement(2943..3803)
/transl_table=11
/note="ampicillin resistance"
/protein_id="SJL86640.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 3939..4316
/note="hCMV-IE enhancer"
/regulatory_class="promoter"
regulatory 4322..5663
/note="AG promoter"
/regulatory_class="promoter"
misc_feature 4691..5615
/note="intron AG promoter"
BASE COUNT 1187 a 1513 c 1587 g 1379 t
ORIGIN
1 ttgaattcca ccatgggtgc gccggtgccg tatccggacc cgctggaacc gcgtgcggcc
61 gcgatgtctg cgggagaccc ccgcgtggga tccgggtccc tggactcctt catgttctcc
121 atacccttgg tcgcgcttaa cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct
181 cggacgcccg tggcggccga ctggaccttg ctcccggagg agatgggctt cgagtacttg
241 gagatccgag agctggaaac gcgccctgac cccactcgca gtttgttgga tgcctggcag
301 gggcgctctg gcgcgtctgt cggcaggctg ctagagctgc tggccttgtt agaccgtgag
361 gatatactga aggagctgaa gtcgcgcatc gaggaggact gccagaaata cttaggtaag
421 cagcagaacc aggagtccga gaagccttta caggtggcca gagtggaaag cagtgtccca
481 caaacaaagg aactgggagg catcaccacc cttgatgacc ccctaggaca aacgccggaa
541 cttttcgatg cctttatctg ctactgcccc aacgatatcg agtttgtgca ggagatgatc
601 cggcaactag aacagacaga ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg
661 ccgggcacct gtgtctggtc cattgccagc gagctaattg agaaaaggtg tcgccgcatg
721 gtggtggttg tttctgacga ttatctacag agcaaggaat gtgacttcca gaccaagttt
781 gcactcagcc tgtctccagg tgtccaacag aagcgactga ttcctattaa atacaaggcg
841 atgaagaagg actttcccag tatcctgcgg ttcatcacta tatgcgacta taccaaccct
901 tgcaccaagt cctggttctg gacccgcctt gccaaggctt tgtccctgcc ctgaagatct
961 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
1021 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
1081 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
1141 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
1201 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
1261 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
1321 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
1381 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
1441 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
1501 gaagcataaa gtgtaaagcc tgggtgccta atgagtgagc taactcacat taattgcgtt
1561 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
1621 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
1681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
1741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
1801 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
1861 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
1921 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
1981 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
2041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
2101 atcagggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
2161 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
2221 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
2281 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
2341 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
2401 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
2461 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
2521 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
2581 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
2641 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
2701 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
2761 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
2821 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
2881 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
2941 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
3001 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
3061 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
3121 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
3181 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
3241 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
3301 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
3361 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
3421 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
3481 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
3541 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
3601 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
3661 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
3721 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
3781 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
3841 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
3901 gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat tgactagtta
3961 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
4021 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
4081 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
4141 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
4201 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
4261 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
4321 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
4381 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
4441 gcccccccca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggaaaggtgc
4501 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
4561 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgttgcgc gctgccttcc
4621 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
4681 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
4741 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
4801 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
4861 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
4921 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
4981 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
5041 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
5101 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
5161 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
5221 ctcgggggaa ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
5281 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
5341 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
5401 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
5461 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
5521 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
5581 ctaaccatgt tcatgccttc ttctttttcc tacagctcct gggcaacgtg ctggttattg
5641 tgctgtctca tcattttggc aaagaa
//