LOCUS LT009449 9402 bp DNA circular SYN 15-AUG-2016 DEFINITION Lentiviral vector pLenti6-eGFP-V5-Blast, complete sequence. ACCESSION LT009449 VERSION LT009449.1 KEYWORDS . SOURCE Lentiviral vector pLenti6-eGFP-V5-Blast ORGANISM Lentiviral vector pLenti6-eGFP-V5-Blast other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 9402) AUTHORS De Schamphelaire W. JOURNAL Submitted (22-DEC-2015) to the INSDC. BCCM/LMBP, Universiteit Gent, Technologiepark 927, 9052, BELGIUM. REFERENCE 2 AUTHORS De Groote P., Grootjans S., Lippens S., Eichperger C., Leurs K., Kahr I., Tanghe G., Bruggeman I., De Schamphelaire W., Urwyler C., Vandenabeele P., Haustraete J., Declercq W. TITLE Generation of a new Gateway-compatible inducible lentiviral vector platform allowing easy derivation of co-transduced cells JOURNAL BioTechniques 60(5), 252-259(2016). PUBMED 27177818 FEATURES Location/Qualifiers source 1..9402 /organism="Lentiviral vector pLenti6-eGFP-V5-Blast" /mol_type="other DNA" /note="BCCM/LMBP Plasmid collection (Ghent University, Belgium) accession number LMBP 8403." /db_xref="taxon:1783456" misc_feature 1..16 /note="HIV-1 cPPT" regulatory 17..251 /note="RSV/HIV-1 dU3/3' LTR" /regulatory_class="terminator" regulatory 323..397 /note="SV40 polyA early" /regulatory_class="terminator" regulatory complement(323..397) /note="SV40 polyA late" /regulatory_class="terminator" rep_origin 533..608 /note="SV40 ori" regulatory complement(642..658) /note="T7 promoter" /regulatory_class="promoter" rep_origin 826..1281 /note="f1 origin" CDS 1412..2272 /transl_table=11 /gene="bla" /product="beta-lactamase" /note="ampicillin resistance" /protein_id="CVH74097.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2813..3215 /note="pMB1 ori" regulatory 3307..3390 /note="lac promoter" /regulatory_class="promoter" regulatory 3454..3470 /note="T3 promoter" /regulatory_class="promoter" regulatory 3579..3907 /note="RSV/HIV-1 5' LTR" /regulatory_class="promoter" misc_feature 4018..4062 /note="HIV-1 psi packaging signal" misc_feature 4572..4805 /note="HIV-1 Rev response element" regulatory 5304..5820 /note="hCMV-IE promoter and enhancer" /regulatory_class="promoter" regulatory complement(5937..6061) /note="gateway recombination site attR1" /regulatory_class="attenuator" CDS 6170..6829 /transl_table=11 /gene="cat" /product="chloramphenicol acetyl transferase" /note="chloramphenicol resistance" /protein_id="CVH74098.1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" CDS 7171..7476 /transl_table=11 /gene="ccdB" /product="ccdB" /note="B gene of the control of cell death locus of the Escherichia coli F plasmid" /protein_id="CVH74099.1" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" regulatory 7517..7641 /note="gateway recombination site attR2" /regulatory_class="attenuator" CDS 7673..8461 /transl_table=11 /gene="EGFP" /product="EGFP" /note="Aequorea victoria green fluorescent protein,enhanced red-shifted variant with V5 epitope tag" /protein_id="CVH74100.1" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKEGPRFEGKPIPNPLLGLDSTRTG" regulatory 8482..8764 /note="SV40 early promoter" /regulatory_class="promoter" rep_origin 8721..8796 /note="SV40 ori" regulatory 8869..8934 /note="synthetic EM7 promoter" /regulatory_class="promoter" CDS 8935..9333 /transl_table=11 /gene="bsr" /product="blasticidin S-resistance" /protein_id="CVH74101.1" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP GIKAIVKDSDGQPTAVGIRELLPSGYVWEG" BASE COUNT 2501 a 2232 c 2364 g 2305 t ORIGIN 1 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt 61 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 121 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 181 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 241 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 301 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 361 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 421 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg 481 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 541 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 601 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg 661 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 721 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca 781 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg 841 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 901 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 961 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 1021 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 1081 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 1141 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 1201 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 1261 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat 1321 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata 1381 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct 1441 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa 1501 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa 1561 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt 1621 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg 1681 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca 1741 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa 1801 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt 1861 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc 1921 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa 1981 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga 2041 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc 2101 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga 2161 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga 2221 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga 2281 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 2341 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 2401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 2461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2581 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 2881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 3001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 3061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 3121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 3181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc 3241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 3301 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 3361 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 3421 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa 3481 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta 3541 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg 3601 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt 3661 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac 3721 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 3781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 3841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 3901 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc 3961 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga 4021 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca 4081 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa 4141 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag 4201 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac 4261 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct 4321 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg 4381 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga 4441 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt 4501 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga 4561 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc 4621 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag 4681 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg 4741 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag 4801 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat 4861 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg 4921 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac 4981 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat 5041 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc 5101 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga 5161 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa 5221 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga 5281 tctcgacggt atcgataagc ttgggagttc cgcgttacat aacttacggt aaatggcccg 5341 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 5401 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 5461 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 5521 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 5581 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 5641 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 5701 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 5761 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 5821 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga 5881 agacaccgac tctagaggat ccactagtcc agtgtggtgg aattctgcag atatcaacaa 5941 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 6001 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 6061 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 6121 gattttgagt taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa 6181 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 6241 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 6301 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 6361 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 6421 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 6481 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 6541 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 6601 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 6661 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 6721 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat 6781 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa gatctggatc 6841 cggcttacta aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag 6901 aatatatact gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt 6961 attacagtga cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata 7021 tctccggtct ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct 7081 ggaaagcgga aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct 7141 cttttgctga cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga 7201 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga 7261 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 7321 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 7381 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 7441 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggctccgt tatacacagc 7501 cagtctgcag gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg 7561 ttttttatgc aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc 7621 agctttcttg tacaaagtgg ttgatatcca gcacagtggc ggccgctcga gtatggtgag 7681 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt 7741 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct 7801 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac 7861 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga 7921 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga 7981 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg 8041 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga 8101 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa 8161 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta 8221 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag 8281 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga 8341 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg agggcccgcg 8401 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta 8461 gtaatgagtt tggaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 8521 ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg 8581 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 8641 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc 8701 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc 8761 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa 8821 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa 8881 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc 8941 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc 9001 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc 9061 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg 9121 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag 9181 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct 9241 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa 9301 ttgctgccct ctggttatgt gtgggagggc taagcacaat tcgagctcgg tacctttaag 9361 accaatgact tacaaggcag ctgtagatct tagccacttt tt //