LOCUS LT009439 8363 bp DNA circular SYN 15-AUG-2016
DEFINITION Lentiviral vector pDG1i-HA-Blast, complete sequence.
ACCESSION LT009439
VERSION LT009439.1
KEYWORDS .
SOURCE Lentiviral vector pDG1i-HA-Blast
ORGANISM Lentiviral vector pDG1i-HA-Blast
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 8363)
AUTHORS De Schamphelaire W.
JOURNAL Submitted (22-DEC-2015) to the INSDC. BCCM/LMBP, Universiteit Gent,
Technologiepark 927, 9052, BELGIUM.
REFERENCE 2
AUTHORS De Groote P., Grootjans S., Lippens S., Eichperger C., Leurs K.,
Kahr I., Tanghe G., Bruggeman I., De Schamphelaire W., Urwyler C.,
Vandenabeele P., Haustraete J., Declercq W.
TITLE Generation of a new Gateway-compatible inducible lentiviral vector
platform allowing easy derivation of co-transduced cells
JOURNAL BioTechniques 60(5), 252-259(2016).
PUBMED 27177818
FEATURES Location/Qualifiers
source 1..8363
/organism="Lentiviral vector pDG1i-HA-Blast"
/mol_type="other DNA"
/note="BCCM/LMBP Plasmid collection (Ghent University,
Belgium) accession number LMBP 9593."
/db_xref="taxon:1783446"
regulatory complement(35..159)
/note="gateway recombination site attR1"
/regulatory_class="attenuator"
CDS 268..927
/transl_table=11
/gene="cat"
/product="chloramphenicol acetyl transferase"
/note="chloramphenicol resistance"
/protein_id="CVH74036.1"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
A"
CDS 1269..1574
/transl_table=11
/gene="ccdB"
/product="ccdB"
/note="B gene of the control of cell death locus of the
Escherichia coli F plasmid"
/protein_id="CVH74037.1"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
regulatory 1615..1739
/note="gateway recombination site attR2"
/regulatory_class="attenuator"
misc_feature 1783..1809
/note="HA epitope"
regulatory 1845..2127
/note="SV40 early promoter"
/regulatory_class="promoter"
rep_origin 2084..2159
/note="SV40 ori"
regulatory 2232..2297
/note="synthetic EM7 promoter"
/regulatory_class="promoter"
CDS 2298..2696
/transl_table=11
/gene="bsr"
/product="blasticidin S-resistance"
/protein_id="CVH74038.1"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 2766..2781
/note="HIV-1 cPPT"
regulatory 2782..3016
/note="RSV/HIV-1 dU3/3' LTR"
/regulatory_class="terminator"
regulatory 3088..3162
/note="SV40 polyA early"
/regulatory_class="terminator"
regulatory complement(3088..3162)
/note="SV40 polyA late"
/regulatory_class="terminator"
rep_origin 3298..3373
/note="SV40 ori"
regulatory complement(3407..3423)
/note="T7 promoter"
/regulatory_class="promoter"
rep_origin 3591..4046
/note="f1 origin"
CDS 4177..5037
/transl_table=11
/gene="bla"
/product="beta-lactamase"
/note="ampicillin resistance"
/protein_id="CVH74039.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
rep_origin 5578..5980
/note="pMB1 ori"
regulatory 6072..6155
/note="lac promoter"
/regulatory_class="promoter"
regulatory 6219..6235
/note="T3 promoter"
/regulatory_class="promoter"
regulatory 6344..6672
/note="RSV/HIV-1 5' LTR"
/regulatory_class="promoter"
misc_feature 6783..6827
/note="HIV-1 psi packaging signal"
misc_feature 7337..7570
/note="HIV-1 Rev response element"
regulatory 8088..8355
/note="hCMVTRE promoter"
/regulatory_class="promoter"
BASE COUNT 2257 a 1893 c 2087 g 2126 t
ORIGIN
1 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagctgaacg
61 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
121 tacataatac tgtaaaacac aacatatcca gtcactatgg cggccgcatt aggcacccca
181 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccggcg
241 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
301 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
361 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
421 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
481 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
541 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
601 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
661 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
721 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
781 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
841 catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
901 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
961 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
1021 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
1081 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
1141 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
1201 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
1261 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
1321 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
1381 gtgcacgtct gctgtcagat aaagtctccc gtgaacttta cccggtggtg catatcgggg
1441 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
1501 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
1561 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
1621 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
1681 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
1741 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtaccccta cgacgtgccc
1801 gactacgcca cgcgtaccgg ttagtaatga gtttggaatt aattctgtgg aatgtgtgtc
1861 agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca aagcatgcat
1921 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
1981 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
2041 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
2101 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
2161 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
2221 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
2281 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
2341 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
2401 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
2461 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
2521 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
2581 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
2641 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac
2701 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
2761 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
2821 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
2881 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
2941 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
3001 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
3061 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
3121 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
3181 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
3241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
3301 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
3361 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
3421 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
3481 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
3541 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
3601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
3661 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
3721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
3781 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
3841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
3901 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
3961 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4021 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
4081 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
4141 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
4201 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
4261 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
4321 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
4381 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
4441 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
4501 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
4561 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
4621 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
4681 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
4741 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
4801 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
4861 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
4921 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
4981 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
5041 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
5161 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
5221 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
5281 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
5341 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
5401 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
5461 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
5521 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
5581 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
5641 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
5701 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
5761 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
5821 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
5881 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
5941 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
6001 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
6061 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
6121 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
6181 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
6241 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
6301 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
6361 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
6421 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
6481 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
6541 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
6601 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
6661 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
6721 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
6781 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
6841 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
6901 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
6961 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
7021 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
7081 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
7141 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
7201 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
7261 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
7321 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
7381 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
7441 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
7501 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
7561 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
7621 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
7681 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
7741 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
7801 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
7861 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
7921 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
7981 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
8041 acggatctcg acggtatcga tggtccgagg ttctagacga gtttactccc tatcagtgat
8101 agagaacgat gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
8161 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
8221 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
8281 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
8341 tgaaccgtca gatcgcaccg gtc
//