LOCUS       LE637941                 231 bp    mRNA    linear   TSA 23-OCT-2015
DEFINITION  TSA: Hynobius retardatus mRNA, contig: comp7813554_c0_seq1.
ACCESSION   LE637941
VERSION     LE637941.1
DBLINK      BioProject:PRJDB2409
            Sequence Read Archive:DRR016729, DRR016730, DRR016731, DRR016732,
            DRR016733, DRR016734, DRR016735, DRR016736, DRR016737, DRR016738,
            DRR016739, DRR016740, DRR016741, DRR016742, DRR016743, DRR016744,
            DRR016745, DRR016746, DRR016747, DRR016748, DRR016749, DRR016750,
            DRR016751, DRR016752, DRR016753, DRR016754, DRR016755, DRR016756,
            DRR016757, DRR016758, DRR016759, DRR016760, DRR016761, DRR016762,
            DRR016763, DRR016764, DRR016765, DRR016766, DRR016767, DRR016768,
            DRR016769, DRR016770, DRR016771, DRR016772, DRR016773, DRR016774,
            DRR016775, DRR016776, DRR016777, DRR016778, DRR016779, DRR016780,
            DRR016781, DRR016782, DRR016783, DRR016784, DRR016785, DRR016786,
            DRR016787, DRR016788, DRR016789, DRR016790, DRR016791, DRR016792,
            DRR016793, DRR016794, DRR016795, DRR016796, DRR016797, DRR016798,
            DRR016799, DRR016800
            BioSample:SAMD00010994, SAMD00010995, SAMD00010996, SAMD00010997,
            SAMD00010998, SAMD00010999, SAMD00011000, SAMD00011001,
            SAMD00011002, SAMD00011003, SAMD00011004, SAMD00011005,
            SAMD00011006, SAMD00011007, SAMD00011008, SAMD00011009,
            SAMD00011010, SAMD00011011, SAMD00011012, SAMD00011013,
            SAMD00011014, SAMD00011015, SAMD00011016, SAMD00011017,
            SAMD00011018, SAMD00011019, SAMD00011020, SAMD00011021,
            SAMD00011022, SAMD00011023, SAMD00011024, SAMD00011025,
            SAMD00011026, SAMD00011027, SAMD00011028, SAMD00011029,
            SAMD00011030, SAMD00011031, SAMD00011032, SAMD00011033,
            SAMD00011034, SAMD00011035, SAMD00011036, SAMD00011037,
            SAMD00011038, SAMD00011039, SAMD00011040, SAMD00011041,
            SAMD00011042, SAMD00011043, SAMD00011044, SAMD00011045,
            SAMD00011046, SAMD00011047, SAMD00011048, SAMD00011049,
            SAMD00011050, SAMD00011051, SAMD00011052, SAMD00011053,
            SAMD00011054, SAMD00011055, SAMD00011056, SAMD00011057,
            SAMD00011058, SAMD00011059, SAMD00011060, SAMD00011061,
            SAMD00011062, SAMD00011063, SAMD00011064, SAMD00011065
KEYWORDS    TSA; Transcriptome Shotgun Assembly.
SOURCE      Hynobius retardatus (Hokkaido salamander)
  ORGANISM  Hynobius retardatus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Amphibia; Batrachia; Caudata; Cryptobranchoidea; Hynobiidae;
            Hynobius; Satobius.
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Matsunami,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2015) to the DDBJ/EMBL/GenBank databases.
            Contact:Masatoshi Matsunami
            Hokkaido University, Graduate School of Environmental Science;
            N10W5, Kita-ku, Sapporo, Hokkaido 060-0810, Japan
REFERENCE   2
  AUTHORS   Matsunami,M., Kitano,J., Kishida,O., Michimae,H., Miura,T. and
            Nishimura,K.
  TITLE     Transcriptome analysis of predator- and prey-induced phenotypic
            plasticity in the Hokkaido salamander (Hynobius retardatus)
  JOURNAL   Mol. Ecol. 24, 3064-3076 (2015)
  REMARK    DOI:10.1111/mec.13228
COMMENT     We carried out two different experiments, a tadpole experiment and
            a dragonfly experiment, each consisting of a treatment group and a
            control group. In the tadpole experiment, 7-day-old salamander
            hatchlings were individually assigned to plastic enclosures
            containing 10 small tadpoles as inducers (i.e., tadpole-exposure
            treatments) or to enclosures without tadpoles (i.e., tadpole
            controls).
            In the dragonfly experiment, the sides of plastic enclosures were
            perforated with small holes and the 10 enclosures were put into
            tanks containing 2 L of aged tap water. Thus, all enclosures in
            the same tank shared the same water. We assigned a 7-day-old
            salamander larva to each of nine enclosures in every tank. In the
            dragonfly-exposure treatment, a dragonfly nymph was placed in the
            remaining enclosure. In the dragonfly control treatment, the tenth
            enclosure was left empty.
            We collected tissue samples for the transcriptome analysis at
            three census time points (0 h, 12 h, and 7 days). We dissected the
            salamander larvae from each experiment and sampled tissues from
            the brain and from those body parts that were changed drastically
            in the experiment (tadpole experiment, head excluding the brain;
            dragonfly experiment, gills and tail). We pooled the sampled
            tissues from 2-5 individuals.
            
            ##Assembly-Data-START##
            Assembly Method       :: Trinity v. 2013-2-25
            Sequencing Technology :: Hiseq2000
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..231
                     /country="Japan"
                     /db_xref="taxon:36312"
                     /dev_stage="larvae"
                     /mol_type="mRNA"
                     /note="contig: comp7813554_c0_seq1"
                     /organism="Hynobius retardatus"
                     /tissue_type="mixture of brain, gill, head, tail"
BASE COUNT           67 a           35 c           34 g           95 t
ORIGIN      
        1 attgataatc aaagcctttt tcagaaatca atggacaata agagtgtatt tgtctatttc
       61 tagagtgtct attttatttt gtattctatt tgtctaccct tgaggaatac cacacagaat
      121 taatagccat caagtctgat agtgtaagtc ctttactttt tcctttcttt ttggaataag
      181 aagttattct cagtctgtag cattttcata taaatttttg gaatcagctt a
//