LOCUS LC744045 18608 bp DNA circular SYN 23-DEC-2022 DEFINITION Cloning vector CAGfshGtACR2-EYFP_R26 DNA, complete sequence. ACCESSION LC744045 VERSION LC744045.1 KEYWORDS . SOURCE Cloning vector CAGfshGtACR2-EYFP_R26 ORGANISM Cloning vector CAGfshGtACR2-EYFP_R26 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 18608) AUTHORS Mukai,Y., Yamanaka,A., Abe,M. and Sakimura,K. TITLE Direct Submission JOURNAL Submitted (19-DEC-2022) to the DDBJ/EMBL/GenBank databases. Contact:Yasutaka Mukai Research Institute of Environmental Medicine, Nagoya University, Neuroscience II; Furo, Chikusa, Nagoya, Aichi 464-8601, Japan URL :http://www.riem.nagoya-u.ac.jp/shinkei2.html REFERENCE 2 AUTHORS Mukai,Y., Li,Y., Nakamura,A., Fukatsu,N., Iijima,D., Abe,M., Sakimura,K., Itoi,K. and Yamanaka,A. TITLE Cre-dependent ACR2-expressing reporter mouse strain for efficient long-lasting inhibition of neuronal activity JOURNAL Unpublished (2022) COMMENT FEATURES Location/Qualifiers source 1..18608 /db_xref="taxon:3015776" /mol_type="other DNA" /organism="Cloning vector CAGfshGtACR2-EYFP_R26" protein_bind 1..21 /bound_moiety="BP Clonase(TM)" /gene="attB4" /note="core recombination site for the Gateway_ BP reaction" misc_feature 64..4785 /note="ROSA26 left arm" protein_bind 4801..4825 /bound_moiety="BP Clonase(TM)" /gene="attB1" /note="recombination site for the Gateway(R) BP reaction" regulatory 4853..5232 /note="CMV enhancer" /note="human cytomegalovirus immediate early enhancer" /regulatory_class="enhancer" regulatory 5234..5511 /note="chicken beta-actin promoter" /regulatory_class="promoter" intron 5512..6529 /note="chimera between introns from chicken beta-actin and rabbit beta-globin" /note="chimeric intron" protein_bind 6611..6644 /bound_moiety="Cre recombinase" /gene="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 6660..6781 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /regulatory_class="polyA_signal_sequence" regulatory 6888..7009 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /regulatory_class="polyA_signal_sequence" protein_bind 7120..7167 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /gene="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." regulatory 7172..7671 /note="PGK promoter" /note="mouse phosphoglycerate kinase 1 promoter" /regulatory_class="promoter" intron 7706..7935 /note="chimera between introns from adenovirus and immunogloblin heavy chain genes" /note="chimeric intron" CDS 7967..8761 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="NeoR/KanR" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /product="aminoglycoside phosphotransferase from Tn5" /protein_id="BDV09305.1" /transl_table=11 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" regulatory 8789..9013 /note="bGH poly(A) signal" /note="bovine growth hormone polyadenyltion signal" /regulatory_class="polyA_signal_sequence" protein_bind 9042..9089 /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /gene="FRT" /note="FLP-mediated recombination occurs in the 8-bp core sequence TCTAGAAA (Turan and Bode, 2011)." misc_feature 9115..10418 /note="STOP cassette" intron 9631..9696 /note="SV40 (simian virus 40) small t antigen intron" /note="small t intron" regulatory 10271..10405 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" /regulatory_class="polyA_signal_sequence" protein_bind 10425..10458 /bound_moiety="Cre recombinase" /gene="loxP" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." regulatory 10498..10507 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" CDS 10504..12102 /codon_start=1 /product="EYFP fused hGtACR2" /protein_id="BDV09306.1" /transl_table=11 /translation="MASQVVYGEWASTHTECYNMSRIDSTFVSLLQLVWAVVSGCQTI FMISRAPKVPWESVYLPFVESITYALASTGNGTLQMRDGRFFPWSRMASWLCTCPIML GQISNMALVKYKSIPLNPIAQAASIIRVVMGITATISPAEYMKWLFFFFGATCLVFEY SVVFTIFQVGLYGFESVGTPLAQKVVVRIKMLRLIFFIAWTMFPIVWLISPTGVCVIH ENVSAILYLLADGLCKNTYGVILWSTAWGVLEGKWDPACLPGQEKPEADDPFGLNHEK NAPPNDEVNIRMFGRAAAVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTI FFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADK QKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEK RDHMVLLEFVTAAGITLGMDELYK" misc_feature 10504..11376 /gene="hGtACR2" misc_feature 11386..12102 /gene="EYFP" /note="mammalian codon-optimized" /product="enhanced YFP" misc_feature 12116..12704 /note="WPRE" /note="woodchuck hepatitis virus posttranscriptional regulatory element" regulatory 12859..12914 /note="beta-globin poly(A) signal" /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" /regulatory_class="polyA_signal_sequence" protein_bind complement(13280..13304) /bound_moiety="BP Clonase(TM)" /gene="mutant version of attB" /note="attB2" /note="recombination site for the Gateway(R) BP reaction" misc_feature 13324..18562 /note="Rosa26 Right arm" protein_bind complement(18588..18608) /bound_moiety="BP Clonase(TM)" /gene="mutant version of attB" /note="attB3" /note="core recombination site for the Gateway(R) BP reaction" BASE COUNT 4448 a 4450 c 4752 g 4958 t ORIGIN 1 caactttgta tagaaaagtt ggggaacaaa agctgggtac gtcgggccgt cacgcgtatg 61 catatttgtg aattcaaaat actctacaat gtttcttcat tttttgctac aaattctaga 121 accattacat ttatgggatc taaataaatt tgtacaaaat taaacagact cccgaaggaa 181 aaggcattgg ttgcagtaat aatcctaggg aggaaaaacc ctacgatttt atgtgctgca 241 catgcctggg tgatggttca gtttcaaata ttccttgcca gacaaactac ttaaaaaggc 301 ttaatcaaag atcaccctgg ttctatttgg ttaggggtag ggtgggctta gaatggactc 361 ttgctgcctt cttagtatct tgaactaaac ttactcaatt tctgaatatt ccttctgtgt 421 ctctgatgga ttactgccaa aacacatctt tgttcggtgg aaagtctata atccaacaca 481 tacacgatta atccaattta taggtcagat atcagtaagt atatctaatc tggagaggtc 541 tacagattaa tttagaatta acaacaaacc aagcattaat ccaaaagcag ctctttttag 601 tttaggcatc caaatatata gtaagatgtt tcaaagaaca tcaatctatt cacaaaggta 661 aatacggcct aatacggaga gacaaataca aatctgaaat gactcaagca attaaaagat 721 ggaggaattc atgtcacttg cactgggaaa acaaaacaaa gatcctcctc accctattac 781 ccacattact atttttaggt tttttagggg ttaaaaaaga tcaaaacact aatgaacttt 841 aagtcctgtg aagggtaaaa cctcagatag taacaaaaag cttccaaccc ctcctcaaac 901 aaaaaacccc aagtctttaa ctttgatcca gttttcagat gctgatatcc ataaatggat 961 acagttatga attgctaatt ctggtctctt cactagcaaa aagcaaagca gctcagcagt 1021 acaatttccc aggaaagcaa gcaaggtttc tttccagcct gagcagccat cactaagtgc 1081 agttccctgc agccaacagc attaatggac gctgcactgc tgtccttccc tggagacagc 1141 agccagcact actcaagctt ctcacgtagc aaccagagct ccagagccag cagctgctgc 1201 cgccttgtat actcactcct gtgatccaac acaggagcaa ccttttcttt accccacccc 1261 cacttcttaa cacacttttt tttggggggg ggggggaaca agtgctccat gctggaagga 1321 ttggaactat gcttttagaa aggaacaatc ctaaggtcac ttttaaattg aggtctttga 1381 tttgaaaatc aacaaatacc aaattccaaa tattcgtttt aattaaacca gcaatgtgga 1441 tataagcatt aagttttagt tttaaaaagg tcaattttcc aaacattcag caatcatatt 1501 taaatttaca gctaggaaca agagccttgg gtcatgtcct accaaagaac ataactcaat 1561 attctacaca tgacaatctg aataacctta aagcctctaa tcccataaca ggccacaaat 1621 tttggacaga gaactaatga tcctcctgag aaaactggaa gaaatccagg gaaaagaaat 1681 tcctgtgtcc tccaaactca gaaatctcta attatgtcag tattctctgc tttagtccta 1741 ggtcagattg cacacatcta aaataacctc ttaaagtttt cctcctagcg acctaaacca 1801 ttattaatat caaattaacc atcaaaacac tttcctctca atatgctgca cacaaacctc 1861 ctcctggaac ctcctccatc tggatcctcc ccaatcaaaa gtataggtat ttaacatata 1921 agcaaggaag taatgtaaac atgaccttgg tcacaaatat gtcatctaaa aacaatttag 1981 tcaaggtatg gaggaaattc gagaacctga atctttttaa gtattttgag cacaggaaca 2041 attggcaaaa ggaatccagg tatagacaaa acccagagcc cagagctctg ggcgaaaaat 2101 gagttgctgg tgaagacgtt acacaagtaa catgagaaag cagaaaatgc aggtcatcca 2161 cgcacccctg acccaggcca gcagggcggg ctgcagcatc agtacacagg agaaagatcc 2221 ttattcctaa gaatgagaaa ggcaaaggcg cccgatagaa taaattagca tagaaggggc 2281 tttcccagga gttaaaactt tccttctgag cgattaccta ctaaaaccag ggcttttgcc 2341 cactaccatt tacctaggat cttggcttgc acggattcat aggggcatat ccctccccct 2401 cttctttaga gtcgttctta aaagatcgct ctccacgccc taggcaggga aaacgacaaa 2461 atctggctca attccaggct agaaccctac aaattcaaca gggatatcgc aaggatactg 2521 gggcatacgc cacagggagt ccaagaatgt gaggtggggg tggcgaaggt aatgtctttg 2581 gtgtgggaaa agcagcagcc atctgagata ggaactggaa aaccagagga gaggcgttca 2641 ggaagattat ggaggggagg actgggcccc cacgagcgac cagagttgtc acaaggccgc 2701 aagaacaggg gaggtggggg gctcagggac agaaaaaaaa gtatgtgtat tttgagagca 2761 gggttgggag gcctctcctg aaaagggtat aaacgtggag taggcaatac ccaggcaaaa 2821 aggggagacc agagtagggg gaggggaaga gtcctgaccc agggaagaca ttaaaaaggt 2881 agtggggtcg actagatgaa ggagagcctt tctctctggg caagagcggt gcaatggtgt 2941 gtaaaggtag ctgagaagac gaaaagggca agcatcttcc tgctaccagg ctggggaggc 3001 ccaggcccac gaccccgagg agagggaacg cagggagact gaggtgaccc ttctttcccc 3061 cggggcccgg tcgtgtggtt cggtgtctct tttctgttgg acccttacct tgacccaggc 3121 gctgccgggg cctgggcccg ggctgcggcg cacggcactc ccgggaggca gcgagactcg 3181 agttaggccc aacgcggcgc cacggcgttt cctggccggg aatggcccgt acccgtgagg 3241 tgggggtggg gggcagaaaa ggcggagcga gcccgaggcg gggaggggga gggccagggg 3301 cggagggggc cggcactact gtgttggcgg actggcggga ctagggctgc gtgagtctct 3361 gagcgcaggc gggcggcggc cgcccctccc ccggcggcgg cagcggcggc agcggcggca 3421 gctcactcag cccgctgccc gagcggaaac gccactgacc gcacggggat tcccagtgcc 3481 ggcgccaggg gcacgcggga cacgccccct cccgccgcgc cattggcctc tccgcccacc 3541 gccccacact tattggccgg tgcgccgcca atcagcggag gctgccgggg ccgcctaaag 3601 aagaggctgt gctttggggc tccggctcct cagagagcct cggctaggta ggggatcggg 3661 actctggcgg gagggcggct tggtgcgttt gcggggatgg gcggccgcgg caggccctcc 3721 gagcgtggtg gagccgttct gtgagacagc cgggtacgag tcgtgacgct ggaaggggca 3781 agcgggtggt gggcaggaat gcggtccgcc ctgcagcaac cggaggggga gggagaaggg 3841 agcggaaaag tctccaccgg acgcggccat ggctcggggg ggggggggca gcggaggagc 3901 gcttccggcc gacgtctcgt cgctgattgg cttcttttcc tcccgccgtg tgtgaaaaca 3961 caaatggcgt gttttggttg gcgtaaggcg cctgtcagtt aacggcagcc ggagtgcgca 4021 gccgccggca gcctcgctct gcccactggg tggggcggga ggtaggtggg gtgaggcgag 4081 ctggacgtgc gggcgcggtc ggcctctggc ggggcggggg aggggaggga gggtcagcga 4141 aagtagctcg cgcgcgagcg gccgcccacc ctccccttcc tctgggggag tcgttttacc 4201 cgccgccggc cgggcctcgt cgtctgattg gctctcgggg cccagaaaac tggcccttgc 4261 cattggctcg tgttcgtgca agttgagtcc atccgccggc cagcgggggc ggcgaggagg 4321 cgctcccagg ttccggccct cccctcggcc ccgcgccgca gagtctggcc gcgcgcccct 4381 gcgcaacgtg gcaggaagcg cgcgctgggg gcggggacgg gcagtagggc tgagcggctg 4441 cggggcgggt gcaagcacgt ttccgacttg agttgcctca agaggggcgt gctgagccag 4501 acctccatcg cgcactccgg ggagtggagg gaaggagcga gggctcagtt gggctgtttt 4561 ggaggcagga agcacttgct ctcccaaagt cgctctgagt tgttatcagt aagggagctg 4621 cagtggagta ggcggggaga aggccgcacc cttctccgga ggggggaggg gagtgttgca 4681 atacctttct gggagttctc tgctgcctcc tggcttctga ggaccgccct gggcctggga 4741 gaatcccttc cccctcttcc ctcgtgatct gcaactccag tctttagcta gagtcgatcg 4801 acaagtttgt acaaaaaagc aggctcggcc agcttgactc actatagggc tcgacattga 4861 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg 4921 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 4981 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 5041 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 5101 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 5161 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 5221 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc 5281 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg 5341 gggggggggg gggggccccc cccaggcggg gcggggcggg gcgaggggcg gggcggggcg 5401 aggcggaaag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg 5461 gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgtt 5521 gcgcgctgcc ttccccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc 5581 tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt 5641 aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag 5701 gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg 5761 tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc 5821 ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc 5881 ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg 5941 gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc 6001 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg 6061 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct 6121 cgggccgggg agggctcggg ggaaggggcg cggcggcccc cggagcgccg gcggctgtcg 6181 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 6241 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 6301 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 6361 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 6421 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 6481 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 6541 cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcttcgcgg tctttccagt 6601 ggttaattaa ataacttcgt atagcataca ttatacgaag ttatggatct cagacatgat 6661 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat 6721 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt 6781 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt 6841 ttaaagcaag taaaacctct acaaatgtgg taggatctca gacatgataa gatacattga 6901 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg 6961 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa 7021 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta 7081 aaacctctac aaatgtggta gatccgatgg ccgctcgacg aagttcctat tccgaagttc 7141 ctattctcta gaaagtatag gaacttctac cgggtagggg aggcgctttt cccaaggcag 7201 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg 7261 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc 7321 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg 7381 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc 7441 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc 7501 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg 7561 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa 7621 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gaccaattcg 7681 ctgtctgcga gggccagctg ttggggtgag tactccctct caaaagcggg catgacttct 7741 gcgctaagat tgtcagtttc caaaaacgag gaggatttga tattcacctg gcccgcggtg 7801 atgcctttga gggtggccgc gtccatctgg tcagaaaaga caatcttttt gttgtcaagc 7861 ttgaggtgtg gcaggcttga gatctggcca tacacttgag tgacaatgac atccactttg 7921 cctttctctc cacaggtgtc cactcccagg tccaactgca gccaccatga ttgaacaaga 7981 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 8041 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 8101 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc 8161 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 8221 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 8281 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 8341 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 8401 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 8461 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt 8521 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 8581 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 8641 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 8701 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 8761 agctctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 8821 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 8881 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 8941 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 9001 gtgggctcta tggcttctga ggcggaaaga accagctggg ggaagttcct attccgaagt 9061 tcctattctc tagaaagtat aggaacttcc tcgacctgca ggcggccatc tcgactcggg 9121 gacaccaaat atggcgatct cggccttttc gtttcttgga gctgggacat gtttgccatc 9181 gatccatcta ccaccagaac ggccgttaga tctgctgcca ccgttgtttc caccgaagaa 9241 accaccgttg ccgtaaccac cacgacggtt gttgctaaag aagctgccac cgccacggcc 9301 accgttgtag ccgccgttgt tgttattgta gttgctactg ttatttctgg cacttcttgg 9361 ttttcctctt aagtgaggag gaacataacc attctcgttg ttgtcgttga tgcttaaatt 9421 ttgcacttgt tcgctcagtt cagccataat atgaaatgct tttcttgttg ttcttacgga 9481 ataccacttg ccacctatca ccacaactaa ctttttcccg ttcctccatc tcttttatat 9541 tttttttctc gagggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac 9601 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt 9661 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa 9721 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca 9781 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga 9841 agggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg 9901 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg 9961 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat 10021 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac 10081 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat 10141 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt 10201 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacatagaa tgaatgcaat 10261 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 10321 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 10381 caatgtatct tatcatgtct ggatctgaca tggtaagtaa gcttataact tcgtatagca 10441 tacattatac gaagttatcg gcgcgccgat atcgaattcg cccttggcgc ggccgccgcc 10501 accatggcct cccaggtcgt gtacggcgag tgggccagca cccacacaga gtgctacaac 10561 atgagcagga tcgacagcac cttcgtgagc ctgctgcagc tggtgtgggc cgtggtgagc 10621 ggatgccaga ccatcttcat gatcagcagg gcccccaagg tgccctggga gagcgtgtac 10681 ctgcccttcg tggagtccat cacatacgcc ctggccagca ccggcaatgg cacactgcag 10741 atgagggacg gcaggttctt tccctggtcc aggatggcca gctggctgtg cacatgcccc 10801 attatgctgg gccagatctc caacatggct ctggtgaagt acaagagcat ccctctgaac 10861 cccatcgccc aggccgccag cattatcagg gtggtgatgg gcattaccgc taccatctcc 10921 cccgccgagt acatgaagtg gctgttcttc ttcttcggcg ctacctgcct ggtcttcgaa 10981 tacagcgtgg tgttcaccat cttccaggtg ggactgtacg gcttcgagtc cgtgggcacc 11041 cctctggccc agaaggtggt ggtgaggatc aagatgctga ggctgatctt cttcatcgcc 11101 tggaccatgt tccccatcgt gtggctgatt tcccccaccg gcgtgtgcgt gatccacgag 11161 aacgtgagcg ccatcctgta tctgctcgcc gacggcctgt gcaagaacac ctacggcgtg 11221 atcctgtggt ccaccgcctg gggagtgctc gagggaaagt gggacccggc ttgcctccct 11281 ggccaggaga agcctgaggc cgacgacccc ttcggactga accacgagaa gaatgccccc 11341 cccaacgacg aggtcaacat cagaatgttc ggcagggcgg ccgccgtgag caagggcgag 11401 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac 11461 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag 11521 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc 11581 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag 11641 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac 11701 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg 11761 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac 11821 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc 11881 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac 11941 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc 12001 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc 12061 gccgccggga tcactctcgg catggacgag ctgtacaagt aagcggccgc ggaacaatca 12121 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt 12181 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc 12241 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc 12301 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg 12361 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc 12421 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg 12481 cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg 12541 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc 12601 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct 12661 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggtc gacttcactc 12721 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca 12781 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg 12841 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt 12901 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg 12961 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt 13021 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata 13081 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt 13141 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac 13201 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacctg cagcccaagc 13261 ttcgagccct atagtgagta cccagctttc ttgtacaaag tggcccgacg tacccagctt 13321 ttgtctagaa gatgggcggg agtcttctgg gcaggcttaa aggctaacct ggtgtgtggg 13381 cgttgtcctg caggggaatt gaacaggtgt aaaattggag ggacaagact tcccacagat 13441 tttcggtttt gtcgggaagt tttttaatag gggcaaataa ggaaaatggg aggataggta 13501 gtcatctggg gttttatgca gcaaaactac aggttattat tgcttgtgat ccgcctcgga 13561 gtattttcca tcgaggtaga ttaaagacat gctcacccga gttttatact ctcctgcttg 13621 agatccttac tacagtatga aattacagtg tcgcgagtta gactatgtaa gcagaatttt 13681 aatcattttt aaagagccca gtacttcata tccatttctc ccgctccttc tgcagcctta 13741 tcaaaaggta ttttagaaca ctcattttag ccccattttc atttattata ctggcttatc 13801 caacccctag acagagcatt ggcattttcc ctttcctgat cttagaagtc tgatgactca 13861 tgaaaccaga cagattagtt acatacacca caaatcgagg ctgtagctgg ggcctcaaca 13921 ctgcagttct tttataactc cttagtacac tttttgttga tcctttgcct tgatccttaa 13981 ttttcagtgt ctatcacctc tcccgtcagg tggtgttcca catttgggcc tattctcagt 14041 ccagggagtt ttacaacaat agatgtattg agaatccaac ctaaagctta actttccact 14101 cccatgaatg cctctctcct ttttctccat ttataaactg agctattaac cattaatggt 14161 ttccaggtgg atgtctcctc ccccaatatt acctgatgta tcttacatat tgccaggctg 14221 atattttaag acattaaaag gtatatttca ttattgagcc acatggtatt gattactgct 14281 tactaaaatt ttgtcattgt acacatctgt aaaaggtggt tccttttgga atgcaaagtt 14341 caggtgtttg ttgtctttcc tgacctaagg tcttgtgagc ttgtattttt tctatttaag 14401 cagtgctttc tcttggactg gcttgactca tggcattcta cacgttattg ctggtctaaa 14461 tgtgattttg ccaagcttct tcaggaccta taattttgct tgacttgtag ccaaacacaa 14521 gtaaaatgat taagcaacaa atgtatttgt gaagcttggt ttttaggttg ttgtgttgtg 14581 tgtgcttgtg ctctataata atactatcca ggggctggag aggtggctcg gagttcaaga 14641 gcacagactg ctcttccaga agtcctgagt tcaattccca gcaaccacat ggtggctcac 14701 aaccatctgt aatgggatct gatgccctct tctggtgtgt ctgaagacca caagtgtatt 14761 cacattaaat aaataaatcc tccttcttct tctttttttt ttttttaaag agaatactgt 14821 ctccagtaga atttactgaa gtaatgaaat actttgtgtt tgttccaata tggtagccaa 14881 taatcaaatt actctttaag cactggaaat gttaccaagg aactaatttt tatttgaagt 14941 gtaactgtgg acagaggagc cataactgca gacttgtggg atacagaaga ccaatgcaga 15001 ctttaatgtc ttttctctta cactaagcaa taaagaaata aaaattgaac ttctagtatc 15061 ctatttgttt aaactgctag ctttacttaa cttttgtgct tcatctatac aaagctgaaa 15121 gctaagtctg cagccattac taaacatgaa agcaagtaat gataattttg gatttcaaaa 15181 atgtagggcc agagtttagc cagccagtgg tggtgcttgc ctttatgcct ttaatcccag 15241 cactctggag gcagagacag gcagatctct gagtttgagc ccagcctggt ctacacatca 15301 agttctatct aggatagcca ggaatacaca cagaaaccct gttggggagg ggggctctga 15361 gatttcataa aattataatt gaagcattcc ctaatgagcc actatggatg tggctaaatc 15421 cgtctacctt tctgatgaga tttgggtatt attttttctg tctctgctgt tggttgggtc 15481 ttttgacact gtgggctttc tttaaagcct ccttcctgcc atgtggtctc ttgtttgcta 15541 ctaacttccc atggcttaaa tggcatggct ttttgccttc taagggcagc tgctgagatt 15601 tgcagcctga tttccagggt ggggttggga aatctttcaa acactaaaat tgtcctttaa 15661 tttttttttt aaaaaatggg ttatataata aacctcataa aatagttatg aggagtgagg 15721 tggactaata ttaaatgagt ccctccccta taaaagagct attaaggctt tttgtcttat 15781 acttaacttt ttttttaaat gtggtatctt tagaaccaag ggtcttagag ttttagtata 15841 cagaaactgt tgcatcgctt aatcagattt tctagtttca aatccagaga atccaaattc 15901 ttcacagcca aagtcaaatt aagaatttct gacttttaat gttaatttgc ttactgtgaa 15961 tataaaaatg atagcttttc ctgaggcagg gtctcactat gtatctctgc ctgatctgca 16021 acaagatatg tagactaaag ttctgcctgc ttttgtctcc tgaatactaa ggttaaaatg 16081 tagtaatact tttggaactt gcaggtcaga ttcttttata ggggacacac taagggagct 16141 tgggtgatag ttggtaaaat gtgtttcaag tgatgaaaac ttgaattatt atcaccgcaa 16201 cctacttttt aaaaaaaaaa gccaggcctg ttagagcatg cttaagggat ccctaggact 16261 tgctgagcac acaagagtag ttacttggca ggctcctggt gagagcatat ttcaaaaaac 16321 aaggcagaca accaagaaac tacagttaag gttacctgtc tttaaaccat ctgcatatac 16381 acagggatat taaaatattc caaataatat ttcattcaag ttttccccca tcaaattggg 16441 acatggattt ctccggtgaa taggcagagt tggaaactaa acaaatgttg gttttgtgat 16501 ttgtgaaatt gttttcaagt gatagttaaa gcccatgaga tacagaacaa agctgctatt 16561 tcgaggtctc ttggtttata ctcagaagca cttctttggg tttccctgca ctatcctgat 16621 catgtgctag gcctacctta ggctgattgt tgttcaaata aacttaagtt tcctgtcagg 16681 tgatgtcata tgatttcata tatcaaggca aaacatgtta tatatgttaa acatttgtac 16741 ttaatgtgaa agttaggtct ttgtgggttt gatttttaat tttcaaaacc tgagctaaat 16801 aagtcatttt tacatgtctt acatttggtg gaattgtata attgtggttt gcaggcaaga 16861 ctctctgacc tagtaaccct acctatagag cactttgctg ggtcacaagt ctaggagtca 16921 agcatttcac cttgaagttg agacgttttg ttagtgtata ctagtttata tgttggagga 16981 catgtttatc cagaagatat tcaggactat ttttgactgg gctaaggaat tgattctgat 17041 tagcactgtt agtgagcatt gagtggcctt taggcttgaa ttggagtcac ttgtatatct 17101 caaataatgc tggccttttt taaaaagccc ttgttcttta tcaccctgtt ttctacataa 17161 tttttgttca aagaaatact tgtttggatc tccttttgac aacaatagca tgttttcaag 17221 ccatattttt tttccttttt tttttttttt ttggtttttc gagacagggt ttctctgtat 17281 agccctggct gtcctggaac tcactttgta gaccaggctg gcctcgaact cagaaatccg 17341 cctgcctctg cctcctgagt gccgggatta aaggcgtgca ccaccacgcc tggctaagtt 17401 ggatattttg ttatataact ataaccaata ctaactccac tgggtggatt tttaattcag 17461 tcagtagtct taagtggtct ttattggccc ttcattaaaa tctactgttc actctaacag 17521 aggctgttgg tactagtggc acttaagcaa cttcctacgg atatactagc agattaaggg 17581 tcagggatag aaactagtct agcgttttgt atacctacca gctttatact accttgttct 17641 gatagaaata tttcaggaca tctagagtgt actataaggt tgatggtaag cttataagga 17701 acttgaaagt ggagtaacta ctccatttct ctgaggggag aattaaaatt tttgaccaag 17761 tgttgttgag ccactgagaa tggtctcaga acataacttc ttaaggaacc ttcccagatt 17821 gccctcaaca ctgcaccaca tttggtcctg cttgaacatt gccatggctc ttaaagtctt 17881 aattaagaat attaattgtg taattattgt ttttcctcct ttagatcatt ccttgaggac 17941 aggacagtgc ttgtttaagg ctatatttct gctgtctgag cagcaacagg tcttcgagat 18001 caacatgatg ttcataatcc caagatgttg ccatttatgt tctcagaagc aagcagaggc 18061 atgatggtca gtgacagtaa tgtcactgtg ttaaatgttg ctatgcagtt tggatttttc 18121 taatgtagtg taggtagaac atatgtgttc tgtatgaatt aaactcttaa gttacacctt 18181 gtataatcca tgcaatgtgt tatgcaatta ccattttaag tattgtagct ttctttgtat 18241 gtgaggataa aggtgtttgt cataaaatgt tttgaacatt tccccaaagt tccaaattat 18301 aaaaccacaa cgttagaact tatttatgaa caatggttgt agtttcatgc ttttaaaatg 18361 cttaattatt caattaacac cgtttgtgtt ataatatata taaaactgac atgtagaagt 18421 gtttgtccag aacatttctt aaatgtatac tgtctttaga gagtttaata tagcatgtct 18481 tttgcaacat actaactttt gtgttggtgc gagcaatatt gtgtagtcat tttgaaagga 18541 gtcatttcaa tgagtgtcag atctagagcg gccgcatcta gctagagcaa ctttattata 18601 catagttg //