LOCUS       LC494434                1404 bp    mRNA    linear   PLN 28-APR-2020
DEFINITION  Calohypnum plumiforme takinomiya CpCYP964A1 mRNA for pimaradiene
            oxidase 2, complete cds.
ACCESSION   LC494434
VERSION     LC494434.1
KEYWORDS    .
SOURCE      Calohypnum plumiforme
  ORGANISM  Calohypnum plumiforme
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Bryophyta;
            Bryophytina; Bryopsida; Bryidae; Hypnanae; Hypnales; Hypnaceae;
            Calohypnum.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Okada,K., Kawaide,H. and Fan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2019) to the DDBJ/EMBL/GenBank databases.
            Contact:Kazunori Okada
            The University of Tokyo, Biotechnology Research Center; 1-1-1
            Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
            URL    :http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/
REFERENCE   2  
  AUTHORS   Okada,K., Kawaide,H., Miyazaki,S., Miyamoto,K., Yamane,H.,
            Hayashi,K., Nojiri,H., Qiu,J., Ye,C., Timko,M.P. and Fan,L.
  TITLE     Identification of functional gene clustering for specialized
            metabolite in bryophyte
  JOURNAL   Unpublished (2019)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1404
                     /country="Japan:Okayama, Takinomiya"
                     /db_xref="taxon:98943"
                     /isolate="takinomiya"
                     /mol_type="mRNA"
                     /organism="Calohypnum plumiforme"
                     /tissue_type="gametophore"
     CDS             1..1404
                     /codon_start=1
                     /experiment="in vivo enzyme assay in yeast"
                     /function="pimaradiene C3 oxygenation"
                     /gene="CpCYP964A1"
                     /product="pimaradiene oxidase 2"
                     /protein_id="BBM89985.1"
                     /transl_table=1
                     /translation="MDPLLGTALLGAGLLALAALLLRKPSTPTGVPTGAFGWPLVGET
                     FQLLAQPRVFVDDRVSRYGAFFATHVFGSPTIYATTPDAAKYFLADAQRLMKPGYPPS
                     VEAIQDPTRVMDGALHSRIRKTLQPSLGPDALHRPQFVAFTDSVARETLRSWEGRLVN
                     THDEMRKYTFSVILHILCSVEPGPESDEMRGHFYDVESGFVSMPIRLPFTNYSRGLQA
                     RDRIFEAMDKMMAQRRTNHNSRANDVLDALMESEVDGVRLTDAQIKGVLMFCLIAGHE
                     TSSSLLTWIVMHLANNPDLWTALQKEHDSIRQTKLSADEALTWSDMKQMQLTNRVIQE
                     ALRISSLVAYVPRIPLDDMSYKGVVLPKDWHVHVDLASIHLNPDIYPEPHKFDPSRFE
                     ATPKPNTFIPFGTGHRICPGNELAKVESLIFLHQLVSTYSWEIISGFKGIEHWPIPRP
                     KGGLNISVKCRRGAYVN"
BASE COUNT          333 a          360 c          344 g          367 t
ORIGIN      
        1 atggacccgt tgctgggcac tgctctactt ggagcgggct tgctggcgct ggccgccctg
       61 ctgttgcgca agccctccac accaactggt gttcccacag gtgcctttgg ctggccgctt
      121 gttggagaga cattccagct tttggcccaa ccccgtgtct tcgtcgatga tcgtgtctcc
      181 aggtatggag ctttcttcgc gactcatgtc tttggatccc caacgatata tgcgacgacg
      241 cccgatgctg ccaagtattt ccttgcagat gcccagaggc taatgaagcc gggttatcca
      301 ccatccgtgg aggctattca agatcccaca cgagtcatgg atggcgcact gcattcccga
      361 attaggaaaa ctcttcagcc ctctttgggt cctgacgcac tacatcgtcc gcaatttgtc
      421 gccttcactg attcggtcgc tcgggaaact cttcgttctt gggaaggacg acttgtgaac
      481 acccatgacg agatgaggaa gtataccttt tctgtgatac tccatatact ctgcagtgtg
      541 gagccaggtc ctgaatccga cgagatgaga gggcactttt atgacgttga gagcggtttc
      601 gtttcgatgc ctatccgttt acctttcacg aactactccc gaggactgca ggccagagac
      661 cgcatatttg aagctatgga caagatgatg gctcagagga gaacaaatca caactctcgt
      721 gcaaatgatg tgctagatgc gctgatggag agtgaggttg atggggtcag gctcacagat
      781 gctcagatta aaggcgtgct aatgttttgt ttaatagcag gccatgagac ctcttcttct
      841 ctcctcactt ggatcgtcat gcatcttgcc aacaatcccg atctttggac tgccttgcag
      901 aaagaacatg actctattcg gcaaaccaag ttgtcagcgg atgaagctct aacctggagt
      961 gatatgaagc agatgcaact cacgaatcgg gtgattcagg aagccttgag gatttcgtca
     1021 ctagtggcat atgttcctcg aattccccta gatgatatga gctacaaagg ggttgtcctt
     1081 cccaaagatt ggcacgtgca tgttgacctt gcctctatcc acctgaatcc tgacatttac
     1141 ccagagcccc acaagtttga tccatccagg tttgaggcta ctcctaaacc aaatactttc
     1201 ataccctttg gaactggaca tcgaatctgc ccgggcaacg agttagcgaa agtggagtcc
     1261 ttaatcttcc ttcatcaact tgtcagcacg tacagctggg agatcatatc tggtttcaaa
     1321 ggaattgagc attggcccat accacgaccc aaaggtggcc tgaatatttc tgtcaaatgc
     1381 agaagaggag catatgtcaa ttag
//