LOCUS       LC494433                1527 bp    mRNA    linear   PLN 28-APR-2020
DEFINITION  Calohypnum plumiforme takinomiya CpCYP970A14 mRNA for pimaradiene
            oxidase 1, complete cds.
ACCESSION   LC494433
VERSION     LC494433.1
KEYWORDS    .
SOURCE      Calohypnum plumiforme
  ORGANISM  Calohypnum plumiforme
            Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Bryophyta;
            Bryophytina; Bryopsida; Bryidae; Hypnanae; Hypnales; Hypnaceae;
            Calohypnum.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Okada,K., Kawaide,H. and Fan,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JUL-2019) to the DDBJ/EMBL/GenBank databases.
            Contact:Kazunori Okada
            The University of Tokyo, Biotechnology Research Center; 1-1-1
            Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
            URL    :http://park.itc.u-tokyo.ac.jp/biotec-res-ctr/
REFERENCE   2  
  AUTHORS   Okada,K., Kawaide,H., Miyazaki,S., Miyamoto,K., Yamane,H.,
            Hayashi,K., Nojiri,H., Qiu,J., Ye,C., Timko,M.P. and Fan,L.
  TITLE     Identification of functional gene clustering for specialized
            metabolite in bryophyte
  JOURNAL   Unpublished (2019)
COMMENT     
FEATURES             Location/Qualifiers
     source          1..1527
                     /country="Japan:Okayama, Takinomiya"
                     /db_xref="taxon:98943"
                     /isolate="takinomiya"
                     /mol_type="mRNA"
                     /organism="Calohypnum plumiforme"
                     /tissue_type="gametophore"
     CDS             1..1527
                     /codon_start=1
                     /experiment="in vivo enzyme assay in yeast"
                     /function="pimaradiene C19 oxygenation"
                     /gene="CpCYP970A14"
                     /product="pimaradiene oxidase 1"
                     /protein_id="BBM89984.1"
                     /transl_table=1
                     /translation="MELSVWLLALGAATVLLYLVSVKPGGRLSLPPGPPTWPIVGSLF
                     YLSSLPHRSMETLAKKYGPIMFMRLGYLNHLVVSNAGMAREILKVHDAEFASRPHSTA
                     GKYVGFDYKDIIFSPYGDHWRLLRKICTTELLTSARLSTFKAGRHDEVAKMVQSITER
                     GRDGELVKMRPILHMLTSNNICRMLFGRRREATDNLIGSVFDQFFDCVSEMVETVGKF
                     NVGDLIPALKPFDLQGIEGHMKRVSKDMETLLARIIREHREMRAQRNVNAKEDEVKLF
                     LDVLLDLDEELEDKSIMAVMVDMLGGGTDTSATTMEWALSELVHHPAIMKRVQEELDV
                     VVGRDRPVREADLPNLHYLQAVIKENFRLHPAVPLTIPHVNPTAAQLQGYDIPANTNV
                     LVSIWAVGRDPATWENPLEFNPDRFLNSDRGVTGTHYEILPFGSGRRGCPGMNLAQLM
                     VQCGLAALLHAFDWSPAPGIKPEDMSMLESFGGACPIAEPLLVVAKPRVSTDVYKTSA
                     "
BASE COUNT          340 a          399 c          431 g          357 t
ORIGIN      
        1 atggagctct ctgtctggct tcttgccctt ggggcggcta ctgttcttct ctatctcgtc
       61 tccgtgaagc cgggaggccg cttgtctctg cctcctggac cgcccacctg gccgattgtc
      121 ggatctttgt tctatctgag ctcacttccc catcgctcca tggaaacgct tgcgaagaag
      181 tacggcccta ttatgtttat gcgcttgggg tatttgaacc atctggtagt atccaatgcc
      241 ggaatggctc gggagatact gaaggtccac gatgcagaat ttgcttccag acctcactcc
      301 acggcgggga agtatgtggg tttcgactac aaggacatca tcttctcgcc ttacggagat
      361 cattggcgtc tgctccgtaa gatatgcacg acggagctcc tcacctcagc gaggctcagc
      421 acgttcaagg ctggacgcca tgacgaggtc gccaagatgg tacaaagcat cacggaacgg
      481 ggtcgagatg gggagctcgt caagatgagg cccatactgc acatgctaac tagcaacaac
      541 atttgccgga tgctgtttgg gaggcgtcgt gaagctacgg acaacctcat cggatcagtt
      601 tttgaccaat ttttcgactg cgtctcagaa atggttgaga ccgttggaaa attcaacgtt
      661 ggcgacctca ttcccgctct gaagccgttc gacttgcagg ggattgaagg acacatgaag
      721 agggtaagca aggatatgga gacgctgctg gcccgcatca ttcgggagca cagggaaatg
      781 agggcacagc gtaatgtgaa tgcgaaggag gacgaagtca agcttttcct ggatgttctt
      841 ctggatctag atgaagaact cgaagacaag tcgatcatgg cagtcatggt ggatatgctt
      901 ggagggggca ccgacacttc tgcgaccacc atggagtggg cattgtctga gctcgttcat
      961 catcctgcta ttatgaaaag ggtccaggaa gagctcgacg tcgttgtagg aagggatcgc
     1021 cctgtacgag aggcggacct tcctaacctg cactacttgc aagcggtcat caaagaaaac
     1081 ttccgtcttc accctgctgt tccgcttacg attcctcatg tgaacccaac agcagctcag
     1141 ctgcaaggct acgacatccc tgccaacacc aacgtgcttg tcagcatttg ggccgtcggt
     1201 cgggacccag ccacatggga gaatcctctt gagttcaacc cagatcggtt cttgaactcc
     1261 gacagagggg tgacgggaac gcattatgag attctgccct tcggctccgg ccgccgtggc
     1321 tgtcccggca tgaatctggc tcagctgatg gtgcaatgcg gcctcgcagc actgctgcac
     1381 gcgtttgatt ggagcccagc gcctgggatt aagcccgaag atatgagtat gttggaaagt
     1441 ttcggaggag cttgcccaat tgcggaaccc ctcctcgtgg tggcgaagcc ccgggtgtca
     1501 acggatgtat acaagacttc agcttag
//