LOCUS LC413281 7220 bp DNA linear SYN 13-MAR-2019 DEFINITION Synthetic construct EGFP-P2A-Six7-FLAG gene, complete cds. ACCESSION LC413281 VERSION LC413281.1 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct other sequences; artificial sequences. REFERENCE 1 (bases 1 to 7220) AUTHORS Fukada,Y., Kojima,D. and Ogawa,Y. TITLE Direct Submission JOURNAL Submitted (17-JUL-2018) to the DDBJ/EMBL/GenBank databases. Contact:Yohei Ogawa The University of Tokyo, Graduate School of Science, Biological Sciences; 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan REFERENCE 2 AUTHORS Ogawa,Y., Shiraki,T., Asano,Y., Muto,A., Kawakami,K., Suzuki,Y., Kojima,D. and Fukada,Y. TITLE Six6 and Six7 coordinately regulate expression of middle-wavelength opsins in zebrafish JOURNAL Proc. Natl. Acad. Sci. U.S.A. 116, 4651-4660 (2019) REMARK DOI:10.1073/pnas.1812884116 COMMENT FEATURES Location/Qualifiers source 1..7220 /db_xref="taxon:32630" /mol_type="other DNA" /organism="synthetic construct" regulatory 1..5281 /note="promoter region of Danio rerio crx gene" /regulatory_class="promoter" CDS 5288..6880 /codon_start=1 /gene="EGFP-P2A-Six7-FLAG" /protein_id="BBF66891.1" /transl_table=11 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELYKGSGATNFSLLKQAGDVEENPGPGSTMFPLPMFTPEQV ARVCENLEETGDIERLGRFLWSLPAAVPGSAGELLNRHESVMRARALVAFHGGNFEAL YQILQSHRFTRESHAKLQDLWLDAHYREAERLRGRPLGPVEKYRIRKKFPLPRTIWDG EQKTHCFKERTRSLLREWYLQDPYPNPSRKRHLAQATGLTPTQVGNWFKNRRQRDRAA SAKNRLQQDSSLLPSGSSPECSSSEHNPLLRGSSPRHPDSPGNSDCSSGPRGTGASTP DISVSSDSEFESELDYKDDDDK" misc_feature 5288..6004 /note="EGFP coding region" misc_feature 6005..6070 /note="P2A coding region" misc_feature 6080..6853 /note="Danio rerio Six7 coding region" misc_feature 6854..6877 /note="FLAG tag coding region" misc_feature 6941..7220 /note="SV40pA coding region" BASE COUNT 2124 a 1505 c 1436 g 2155 t ORIGIN 1 ttcatggaaa tggcaaaaac attcaattta ttttaatgaa tgtaattata atttttattt 61 aataaataaa taaatcatat taatggtttt aattattaaa attaattcaa tgccacaaga 121 acaagaggtg ttacatttca cagtgttctg aaagagaaaa aagtacatga aaataaatgt 181 tttcagagca ttacacgttt tcagtgcttt tatgtgtgat tgatctaaaa gatgtgcata 241 aatttgaagc catttacaaa atgagaaaag tttgcactga gtttactttt tcttatgtat 301 ataatatttt cctaataaga taaacaaatt aaaaatatgt ggtcctttaa gttctcatta 361 tcagaataaa acagtacatc aaataaacaa atttatatct tatactcctt ttttttttgt 421 tagtaaacaa ttccaaatat ttccaaaata ctcttgagta aataatgaaa aaaaagatgc 481 aaaattgttt ctctttcttg accacaaatt gagataaaat ggacagaatc tccactagag 541 ggccggacaa gttgtcaatc ggttattggg gctcttttca gcatacagcc ccactgacat 601 taaaaccctt ataatctatg atttaaagtc tgtcccggat agcacaaaat gtctttatca 661 gcaaaaaact gcctcatcac ggctttttgt agaaaaaaac tttaagatac tgatttataa 721 tcgtctcacc attaaaaata tgttagaaat tgatttaaat cgtgtgtgag gtattaaatg 781 gctcagaaaa agagtccgac tttaaagtta ttgatgaatt tgttgactgg ataagcagaa 841 tttattaatt ttttaacata tgcatcttta actcgcattg ttgagagtgt gcgcgcgcgt 901 gtgtgtgcgt gtgtccggcg tgtgcgtttc ttcagggatt agaattgtgt gagtgattgg 961 tgctgacagc ggtctgtgta ttactggctg gcctgcagat taagccgctc ctttttagac 1021 tgcctaatct ggattaactg atgcagtgtg cctgcaatga tgcggcccac tacccctctc 1081 tctccctccc tccctctctc tctttctctc tatctatctg tcacctcact cgccctcctt 1141 caccccaatc catcttcaca tttttctttc cgttaacttt atttgcgctg gaacattttg 1201 tttgtttcgt ctatctaact ctctgctcag attaaatatt ttaggcagtg tcagtgtttt 1261 tgctgtgtat ttctgtgcta atgtgttctg gtgtgtgtgc gcgcgcgctc ccgcggatta 1321 gagtttagtc cacttccata attgctcttg tgcctgcgta gctggttgtt gccagatgga 1381 cggactggtt ccccccaccc cccgctgcac caccctccat aaaacttcag cttctgcaag 1441 cacacatgcc gcatccagaa cacatacaaa catcctatag tgacacacaa caacaacatc 1501 attttactgc atttacataa aaacaataac aacaacaact ggtaaaacaa aaaactggca 1561 tcgtttatgt tttgataatc tgagcaaagc attaaaacgc ttcagtagct tcagtattag 1621 tttgtcacat aaataaacag ataaacattt ttaaacatgt tgatattcgt ctatcctatg 1681 gttgttaatt ctgtgttcgt aagtaagaaa ataagtggta caaatgaagt gtgagtggta 1741 caaatgaatg attaaaatat aatgttaata aaaccagctt ttattgtatg aaaatataaa 1801 tagcaaattt gttttgtata aatagtgtct atttcactgt cttcttgttt aaatcatcat 1861 ttaaaaacat atttggctaa tgtttttttt tttttttgta aatagacaag gtaagttcaa 1921 tagatcgtat tattatgttt ttcttttctt tttcaatctt tcataacctg ttttaacaaa 1981 ttttaatctg tttcaatcac ttttattatt atttgttttt actttcttat gctttctcta 2041 ttgtttatgt aaagcacttt gaagtgccac tgtgtaagaa atgcgctata taaacaaact 2101 tgccttgcaa tatcatttat acaattatac ttatcttctt atacaagaat aaaaattaat 2161 tcattctttt gagaaaaaac tagattggta actggaaact tttttatttc gggaaacaaa 2221 ctatgatgca tttcaggccc actaataaag atattatttt aatttcataa ttgcagggct 2281 caacatgatg tattacaata aacaaatttc ccatattaat ttgtgtttgt attttcaaat 2341 tataaaccat aacctgggag aaaatgaaca gttttaattt ttaagaatcc attcatatat 2401 tttcttttca gcttagtccc tttatttatc aggagttgca acagcggaat gaaccagcat 2461 caaccgcata tgttttcgca gagtaattta gttaaatgat ttacggtcaa ttaagtcttt 2521 caattgaact atagcgcatg tgtttggact gtgggggaaa cacggaaacc acgcgaacac 2581 ggggagaaca tgcaaactcc acccagaaat gcgtactggt ccagcctggg ctcgaacaag 2641 caaccttctt gctgcgaggc gacgatgcta accactgcac aatcgtgtcg ccccatttta 2701 aataatgtat ggtaaaatac tcttaattca gaacaagaaa gcgatgtgcc tgtcaagatt 2761 agtctgtgct gtattttcat cacataatta ttttcaaatg actcacacct tcccttaata 2821 tttatttgtc atcgggtaga gcaaactgaa tttaaagtaa cagcactgac aagtcacatt 2881 attgttctta ttttgccatt aagtggtcag tctctcgttc ttattttcac aaacggtttt 2941 atttcaatgc aactaaatta atcaagtatt tgtgctattt taagagaaca tacagcagta 3001 ttttggggaa actatatacg ttttgttgcc ctttgcctgt ggagtttgtt cagtaaggag 3061 agatgacatt tacctcagtc acagccaaat aaccctgtct gcactctgtc tgttttaagc 3121 cagcctatca cattcatttc atcttgagtg ccagttgacg cctttctctc tccctttccc 3181 ttgctcagtc cttaacttgt ttactctccc aacaagtaat actatcaaat ataacatagc 3241 ataccaagtt ctctttttgc ggattacatc atatctcaaa tttcagacag ctccattcaa 3301 gcaaacgtgg tcgagatcct ctcctccgtc aacatacttg cgcttttctt tttcccccgt 3361 ttatgtggcc aagatgttct tcttttatgg ttgatgaaga aaacaagctg aagacatctt 3421 gaccatttat ccacactttc gacttgtatt tttcccactt tgagtccttt agtattttta 3481 tttattttct tcttcaccat attctgttta cacatggtgt ctggtactct cctcgtatag 3541 actgatgata aatctctctt tcctcgcttt atccagggtt aagagacttt tcacaaagca 3601 tattatgata attcagcagt ttgtgtgtgt gtgtgtgtgt gtgtgtatgt gtgcgggatt 3661 caattctagg taaaatagta aagagtaaag atttttaaac atatttcggt atcaaataaa 3721 agattgaaaa ctttaatcag gcattcaatt aaattcacct acaactaaat gatggctgta 3781 gataaaatta taatatctaa atatttaaaa gtgcagagca acaaacaaaa cacatcatta 3841 aggacaactt attttgcttc tttcatcgtg gaaaacattc gattaacaaa agcatattca 3901 gacctttatc gaggtcacta taagcctatt gtaaaccctt ttggaattta tacatataaa 3961 tacggataaa ctgcacagcc aataaacgaa aaaagcaaca ttaacgttaa tttaaaagtg 4021 agaatgttta atagaaagta aaaattcttt tctaacaaag tttatttaca taagtgaaca 4081 ttcaacagaa agtttttttg cttctattgc attaaagtct ttttacaaaa ttgtaatgct 4141 cttcacactt agaaagagac ttatatggtt tatatagttc tataattatg tattattatg 4201 tataattatg tatttttgat tttcatttta tgcctttatt gtggcacatt tttctaatag 4261 gctagtcaaa ctcacagctc gtttcagcca tatatgtgaa tgactgtctt tgactgagtc 4321 aatttattaa tagtgggtta ataaaacaga taaatgcaca gctaaaaaat agcccactaa 4381 aaataatctc taggaattac cataggggaa tttgcttcta taatttaaat atatctgtgg 4441 tattttgagg gatcttttta gtggcatgtg gtaattttca gccatacatt aatggattcg 4501 ccaaattaag ttattttcga tatcgcatta tttatatact tcgattggtt ccaaaatacg 4561 aacttagagt gtaaatctac aggatatttt aaacaaaaca tacaattcag ttaaaaatag 4621 gttagtaatc catctcataa ccacttgatt ttcttgtaat atcatattaa tttctgtttt 4681 tttcctttat attttaagca aacaaaacat ggttatttct tgagattgcc tcccacgtgt 4741 taagctacac gtcaacgtgc gtgtgtgtgt gtgagagaga gagagagaga gagagagtaa 4801 tagagagaga gagagagaga gagagagaga gagagagaga tgtctcttga ttagctgctc 4861 ggtctcttat tgctcatagt gtcccgctcc tgtccggtct tacccaaggg acacgcgcct 4921 ttaccaacgc caaaactctg accaagagcc agcaactttt gtgcatatag acatttattt 4981 gttctttaac tcacattaag ggtctattat tggatattta gagcgatagt aatggcagat 5041 tggtagttaa acttaggcaa aagtgtaagc ttattgaaga cagcgaggag cgcgcgcgcg 5101 agcatctaca ggtgtttgag gagcacagac acgcgccagg cgcgagcaca gacacgcgac 5161 ccccgacccg taaaacagcg tcgcagagaa aaaagagagt gagagagggg tgagagaggg 5221 agggagagag agagatagag agcaatagag agagtgtgcg agagacgcgg ccgtcccaag 5281 agccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 5341 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 5401 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 5461 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 5521 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 5581 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 5641 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 5701 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 5761 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 5821 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 5881 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 5941 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 6001 caagggaagc ggagctacta acttcagcct gctgaagcag gctggagacg tggaggagaa 6061 ccctggacct ggatccacca tgtttcctct gccgatgttc acacctgagc aggtggcccg 6121 agtgtgcgag aatctcgagg aaacaggaga tatcgagcgc ctgggccggt ttctctggtc 6181 tctgcccgcc gccgtgcccg gttccgccgg agagttgctc aaccgtcatg agtcggtgat 6241 gcgcgcgcgg gctctcgtcg cgttccatgg cgggaatttc gaggcgctct atcagattct 6301 gcagagtcac cggtttaccc gcgaatctca cgctaaactg caggacctgt ggctggacgc 6361 gcattaccgg gaggcggagc gcctgagggg cagaccactc ggcccggtag agaagtaccg 6421 catccgtaag aagttccccc tgccgaggac aatctgggat ggagagcaga agacacactg 6481 ctttaaggag agaacccgca gtctgctgag ggaatggtac ctgcaggacc cttatccaaa 6541 cccgtccaga aagcgtcatt tggctcaggc cactggtctc actcccacac aggtgggcaa 6601 ctggttcaag aacaggagac agcgggacag agcagcttct gccaaaaaca gactacagca 6661 ggattcctct ctcctgccgt ctggaagttc tcctgaatgt tcatcatcgg agcacaaccc 6721 actcctgcga ggctcatccc cccgccaccc tgacagcccg gggaacagcg actgcagctc 6781 aggccctcgg gggaccggag cttccacgcc ggacatctcg gtcagcagcg acagcgagtt 6841 tgagtcggaa ttggattaca aggatgacga cgataagtga caattcaagg cctctcgagc 6901 ctctagaact atagtgagtc gtattacgta gatccagaca agctcgaatt aattcatcga 6961 tgatgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca 7021 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat 7081 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg 7141 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta 7201 tgatcctcta gatcagatct //