LOCUS LC029806 4772 bp DNA linear BCT 14-OCT-2015
DEFINITION Enterococcus faecalis enfT, enfA49, enfB, enfC, enfI genes for ABC
transporter, enterocin F4-9 precursor, hypothetical protein,
glycosyltransferase, bacteriocin immunity protein, complete cds,
strain: F4-9.
ACCESSION LC029806
VERSION LC029806.1
KEYWORDS .
SOURCE Enterococcus faecalis
ORGANISM Enterococcus faecalis
Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
Enterococcus.
REFERENCE 1 (bases 1 to 4772)
AUTHORS Zendo,T., Maky,M.A., Ishibashi,N. and Sonomoto,K.
TITLE Direct Submission
JOURNAL Submitted (19-FEB-2015) to the DDBJ/EMBL/GenBank databases.
Contact:Takeshi Zendo
Kyushu University, Bioscience and Biotechnology; 6-10-1 Hakozaki,
Higashi-ku, Fukuoka, Fukuoka 812-8581, Japan
REFERENCE 2
AUTHORS Maky,M.A., Ishibashi,N., Zendo,T., Perez,R.H., Doud,J.R., Karmi,M.
and Sonomoto,K.
TITLE Enterocin F4-9, a Novel O-Linked Glycosylated Bacteriocin
JOURNAL Appl. Environ. Microbiol. 81, 4819-4826 (2015)
REMARK DOI:10.1128/AEM.00940-15
COMMENT
FEATURES Location/Qualifiers
source 1..4772
/db_xref="taxon:1351"
/isolation_source="Egyptian salted-fermented fish"
/mol_type="genomic DNA"
/organism="Enterococcus faecalis"
/strain="F4-9"
CDS 176..2236
/codon_start=1
/gene="enfT"
/product="ABC transporter"
/protein_id="BAR87968.1"
/transl_table=11
/translation="MKTIKLVEQLQESDCGLACLSMLFGYYDYHISLKDLKKEYLISQ
QGLSFNDLIFISNEYGFDLMGFSVGKNTNLEQLSSECPFIANWDQGTHYVVVERIKNG
KYVILDPNACRIILNNDEFKEAFNNKLLNVMKPNNVKRKPDKNGYNAITNMLKEMLTK
NNVFLFLFFIIGSLSQLINVLPPLIYGNLLDKVIAEDYSINAKVILTIFGLIFSLFLV
TIVQEFIKIKLEYNMDYSLLTKYYKHLINLPISFFKNKNQGELIYRFNLLEKFKEIVV
THGIGAIFSIALVIIYSVLMLSISVEMGLVVIILAIFFIIFLTMFSNITIMLSKKVVL
SNAKSQEYLAESIRLIDVIKLNSQENNVEKEMNTLLDKYLLNTKKGRIISTLIDSGVS
TLKVVQTMITFLIGLYFLKNGFITIGQLLSFVVLSESFFIPIYSLIDSYFFMVNLSTT
FSKVSEVLEEKSEEQINDKLQEIDNIVGSIEFKNVYFRYSSFGSYVLENFSLVVNPGD
TIRILGDSGTGKSTIIKLLVGYYQIESGEILIDNKDISKLNKKSYREKISVILKESQL
FSGSIKENVIGNDKVEWEDSDLFIKEAIYNILSQGGLNFDTRVSEGGNNLSEGQKQRV
LIAKSLYKNSKILIWDEATNSLDKVSEDYIFNILRQIEATKIIISHDEIALNPDRDIC
LSNY"
CDS 2306..2509
/codon_start=1
/gene="enfA49"
/product="enterocin F4-9 precursor"
/protein_id="BAR87969.1"
/transl_table=11
/translation="MGNSILNKMTVEEMEAVKGGNLVCPPMPDYIKRLSTGKGVSSVY
MAWQIANCKSSGSCMKGQTNRTC"
CDS 2574..3035
/codon_start=1
/gene="enfB"
/note="transport accessory protein or thiol-disulfide
isomerase"
/product="hypothetical protein"
/protein_id="BAR87970.1"
/transl_table=11
/translation="MKKIFFVLIILSIIGSLILIFRPQKYYEEISVDKFNELKDQKQN
FVIYIGRDDCNDCLSFEPTFHEVIKNNNYEKDIKYVNVKEIRKQSKEGWKEFKENNHF
DQTPVIIHYEHGKIVDKIEWDAEKGLPKSELEDWLLRNILITQGELNDRIE"
CDS 3019..4227
/codon_start=1
/gene="enfC"
/product="glycosyltransferase"
/protein_id="BAR87971.1"
/transl_table=11
/translation="MIELNDYILGYSNSYENELKSKSISNIGDTCKISFLIMTYNEER
CIQRCINSINPLANEIIIIDTGSTDNTLNVISELKNKKVKLFKETWVDDFSYMRNQLI
KKANNDWMFMIDADEFIGPVSLEKLSTLINIVETSNVVPKVISPRLTNTDGSSLYITQ
RLFKRHTQLKYFGYAHEELRFYNDPIFPYICIDLPFYHDGYDNEIVEEKNKNDRNLRL
LKKMLESEPNNLRWVFFCAREMLSLNYASECIKEMVITGLKNSKDDFPVLKSGLYSIL
METSMESDEAYNEYQKNALECNPNNIDIYYLDALKKFNELILEGNNYISKSQNEILSL
KETDSFIHSEGDHLFYLWGKIHFSLQNYKQAFLWWNKIGAYNIRNDVIRELKEINADI
SKFIESYQKT"
CDS 4390..4686
/codon_start=1
/gene="enfI"
/product="bacteriocin immunity protein"
/protein_id="BAR87972.1"
/transl_table=11
/translation="MSSLKWYSGGDDRAKKAEKIIIDLLNDLEYDLGNESLQEVLSSC
LEELRKKGASVPLILSRMNLDISKAIRNDGRKLSESQSIKLKGLTTMSNIRYGY"
BASE COUNT 1822 a 543 c 845 g 1562 t
ORIGIN
1 cggacagctc tcaaaatgat caatttctaa aagaacactt aggagataac tatcagggaa
61 cacctgctgt atatttttat aaagaaggga aactagtaga tcattttatt ggttctcaag
121 atgattctgt agttgataaa tatttaaaga aatacatcag tatggagaaa gctaaatgaa
181 aactattaaa ctagtagaac aattacaaga atcagattgt ggacttgcat gtttatcgat
241 gttattcgga tattacgact accatatatc gttaaaagat ttaaaaaaag agtacttgat
301 ttcacagcaa ggtttgtcgt ttaatgattt gatatttatt tcaaatgaat atgggtttga
361 cttaatgggt ttttctgttg gaaaaaatac aaatttggaa cagctctctt ctgagtgtcc
421 gttcattgca aattgggatc agggaacaca ttatgttgta gttgaaagaa ttaaaaatgg
481 aaagtacgta attttagacc caaatgcatg tagaattatt ttaaacaatg atgagtttaa
541 agaagctttt aacaataagt tgttgaacgt tatgaaacct aataatgtaa aaagaaaacc
601 agataaaaat gggtacaatg caataacgaa tatgctcaaa gaaatgctga ctaagaataa
661 tgtgtttctc tttctatttt ttattatagg ctctttaagc caactaatta atgttttgcc
721 acccttaata tatggtaatt tgttagacaa ggttattgca gaagattaca gtataaatgc
781 caaggtaata ttgacgattt tcggtttaat tttctctctt tttttagtaa ctatagtgca
841 agaatttatt aagattaaac ttgaatacaa catggactac tctttgttaa ccaaatatta
901 taagcactta attaacttac ctattagttt ttttaaaaat aaaaaccaag gtgaactgat
961 atatcgcttt aacttgttag aaaagtttaa agaaattgta gtaacacacg gaattggtgc
1021 aattttcagc attgctcttg tgattatata tagtgttctt atgctatcaa tttctgtgga
1081 aatgggttta gtggttatca ttcttgcaat cttttttata atatttttga caatgttttc
1141 gaacattaca ataatgcttt ctaaaaaagt agtactatct aatgctaaat cacaagaata
1201 cttagcagaa agtataagac ttattgatgt tataaagttg aatagtcagg aaaacaatgt
1261 agaaaaagaa atgaatactt tattagacaa gtatttgctt aatacgaaaa aaggtagaat
1321 tatttctact ttaattgatt caggagtatc tacattaaaa gtggtacaaa caatgataac
1381 ttttttaata ggtttatatt ttctgaaaaa tggatttata actataggac agctattatc
1441 ttttgttgtt ttatctgaat cattttttat tcctatatat tctttaatag acagctattt
1501 ctttatggta aatctctcaa caactttttc aaaagtaagt gaagttttag aagaaaagag
1561 tgaagaacag atcaatgata agctacaaga aatagataat attgttggca gtatagaatt
1621 taaaaacgta tattttagat actcttcttt tggatcatat gtgttagaaa atttttcatt
1681 ggtagtgaat cctggtgata caatacgtat attgggggac tctggaactg gtaagagtac
1741 aataattaag ttattggtgg gatattatca aatagagagc ggggaaattc ttattgataa
1801 taaagatatc tcgaaattaa ataaaaaaag ttatagggaa aagatttctg ttattcttaa
1861 agaaagtcaa ctttttagcg gaagtatcaa ggaaaatgtg ataggaaatg ataaagttga
1921 atgggaagat agcgatttat ttataaaaga agcaatatat aatatattgt cacaaggtgg
1981 tttaaatttt gatacaagag tttctgaggg cggaaataat ttgtctgagg ggcaaaaaca
2041 aagggtgtta atagctaaat cactgtataa gaatagcaaa atactaattt gggatgaagc
2101 aaccaactca ttagataaag taagcgaaga ttatattttt aatattttga gacaaataga
2161 ggctacaaag attataattt cacatgacga aatagctctt aatccagata gagatatttg
2221 tttatctaat tattagtaaa ggttatagac agtaacattg taggtgtgat atcataaatg
2281 taacttaaag agaggcggtg acattatggg taatagtatt ctgaataaaa tgacggttga
2341 agaaatggaa gcggttaaag gcggaaatct tgtttgtccc ccaatgcctg actatatcaa
2401 acgtttatca accggtaaag gtgtgagctc tgtttacatg gcatggcaaa ttgctaattg
2461 taaaagtagt ggttcatgca tgaaaggtca aactaacaga acttgttaat acttaaaaaa
2521 aggctgaatt ttcaattcag ctttttttgt agaatagata ctaggaggaa tcgatgaaaa
2581 aaatattttt tgttttaatt attctatcaa tcataggaag tctcatatta attttcagac
2641 cacaaaagta ctatgaagaa atatctgttg ataaatttaa tgaactaaaa gatcaaaagc
2701 aaaattttgt aatatatatt ggacgcgatg attgcaacga ttgcttgtct tttgaaccaa
2761 catttcatga agtcataaaa aataataact acgaaaaaga tattaagtat gtaaacgtaa
2821 aagaaataag aaagcagtct aaggaaggat ggaaagagtt taaagaaaat aatcacttcg
2881 atcagacccc agtaattatt cattatgaac atggaaagat tgttgataaa attgagtggg
2941 atgcagaaaa gggtttgcct aaatcagaat tagaagactg gttacttaga aatattttaa
3001 ttacacaagg agaactaaat gatagaattg aatgattata ttttaggata ctcgaattca
3061 tatgaaaatg agctaaagag caaaagtata tctaatatag gtgatacctg caaaataagt
3121 tttttaatta tgacttataa cgaagaaaga tgcatacaga ggtgtattaa tagcattaat
3181 cctttggcaa atgagattat aattatagat accggatcta cagataatac attaaatgta
3241 atctcagaac tgaaaaacaa aaaagtaaaa ctttttaaag aaacttgggt agatgacttt
3301 tcttacatga gaaatcaatt aataaaaaaa gcaaacaatg attggatgtt tatgattgat
3361 gccgatgagt ttattgggcc tgttagtcta gaaaaattat ctacgttaat aaatatagta
3421 gaaacttcaa acgtcgttcc taaggtgatt agtcctcgat taacgaatac ggacggctct
3481 tctctttata ttactcagag actttttaaa aggcatacac aattaaaata ttttgggtat
3541 gctcacgaag aattacgatt ttataatgac ccgatttttc catacatctg tattgactta
3601 ccattttatc atgacggata tgataatgaa attgtagaag aaaaaaataa gaacgataga
3661 aatttaagat tactaaaaaa aatgttagaa tctgaaccta ataacttgag atgggtattt
3721 ttttgtgcta gagagatgtt atctttgaat tatgcaagtg aatgtataaa agaaatggta
3781 ataacgggat tgaaaaatag taaggatgat ttccctgtat taaagtctgg attgtattct
3841 attcttatgg aaacaagtat ggaaagtgat gaagcttata atgagtatca aaagaatgcc
3901 ttagagtgta atccaaataa tattgatatt tattatctag atgcgctaaa aaaatttaac
3961 gaactaatat tagagggaaa taattacatt agtaaaagtc aaaacgagat actaagcctt
4021 aaagaaacag actcctttat acattctgaa ggtgaccacc ttttttatct ttgggggaaa
4081 attcactttt ctttgcagaa ttataaacag gcatttttat ggtggaataa aataggtgcg
4141 tataacatac gcaacgacgt tatcagggag ttaaaagaaa taaatgcgga tataagtaaa
4201 tttatcgaaa gttatcaaaa aacataaagt taagacataa ggtagatgta caactttcaa
4261 aaaagtaccc acaaggtcgg cactgtgggt attttttctg cccttataga tattaaagag
4321 aaaacgtagt atatttttgt gaggatttta ttttactttt gacagtaaac attaaggagg
4381 ctaaattata tgagttcatt aaagtggtat tctggtgggg acgacagagc aaaaaaagca
4441 gaaaaaatta ttattgattt gttaaatgac ttagaatacg atctaggtaa tgagtcactt
4501 caggaagtat taagttcttg cttagaagaa ttaagaaaaa aaggagcgtc tgtacctcta
4561 attcttagcc gtatgaatct agatatttct aaagcaatta gaaatgatgg aagaaaatta
4621 tcagaaagtc aatcaatcaa gttaaagggc ttaacaacta tgtctaatat tagatatggc
4681 tattgatgac tcctcgattt agtatctaaa aagtaaaaat gataaaaaga gattagagat
4741 atttgttctt tttaccaatt ttatgtttta ct
//