LOCUS LC018350 3407 bp DNA circular SYN 10-OCT-2015 DEFINITION Expression vector pPAL DNA, complete sequence. ACCESSION LC018350 VERSION LC018350.1 KEYWORDS . SOURCE Expression vector pPAL ORGANISM Expression vector pPAL other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3407) AUTHORS Kobayashi,H. TITLE Direct Submission JOURNAL Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases. Contact:Hideki Kobayashi Agency for Marine-Earth Science and Technology, Institute of Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL :http://www.jamstec.go.jp/j/ REFERENCE 2 AUTHORS Kobayashi,H. TITLE Inducible Suppression of Global Translation by Overuse of Rare Codons JOURNAL Appl. Environ. Microbiol. 81, 2544-2553 (2015) REMARK DOI:10.1128/AEM.03708-14 COMMENT FEATURES Location/Qualifiers source 1..3407 /db_xref="taxon:1602147" /host="E. coli" /mol_type="other DNA" /note="Coding f1 phage inducible lgfp" /organism="Expression vector pPAL" CDS 200..916 /codon_start=1 /gene="lgfp" /product="GFP" /protein_id="BAQ25593.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG IKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM ILLEFVTAAGITHGMDELYK" CDS 1444..2304 /codon_start=1 /gene="Amp" /product="beta-lactamase" /protein_id="BAQ25594.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 2327..3249 /note="ColE1-derived plasmid replication origin" BASE COUNT 935 a 802 c 875 g 795 t ORIGIN 1 aagcttcatg atgaaattcg ccacttgtta gtgtaattcg ctaactcatc ctggcatgtt 61 gctgttgatt cttcaatcag atctttataa atcaaaaaga taaaaaattg gcacgcaaat 121 tgtattaaca gttcagcagg acaatcctga acgcagaaat caagaggaca acattaccgg 181 caggaggttt tttaccggta tgtcaaaggg ggaggagcta ttcacagggg tagtacccat 241 actagtagag ctagacgggg acgtaaatgg gcaaaagttc tcagtatcag gggaggggga 301 gggggacgct acatacggga agctaacact aaagttcata tgtacaacag ggaagctacc 361 cgtaccctgg cccacactag taacaacatt cgggtacggg gtacaatgtt tcgctaggta 421 ccccgaccac atgaagcaac acgacttctt caagtcagct atgcccgagg ggtacgtaca 481 agagaggaca atattctaca aggacgacgg gaattacaag acaagggctg aggtaaagtt 541 cgagggggac acactagtaa ataggataga gctaaagggg atagacttca aggaggacgg 601 gaatatacta gggcacaaga tggagtacaa ttacaattca cacaatgtat acataatggc 661 tgacaagccc aagaatggga taaaggtaaa tttcaagata aggcacaata taaaggacgg 721 gtcagtacaa ctagctgacc actaccaaca aaatacaccc ataggggacg ggcccgtact 781 actacccgac aatcactacc tatcaacaca atcagctcta tcaaaggacc ccaatgagaa 841 gagggaccac atgatactac tagagttcgt aacagctgct gggataacac acgggatgga 901 cgagctatac aagtaggcta gcttggctgt tttggcggat gagagaagat tttcagcctg 961 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt 1021 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat 1081 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa 1141 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct 1201 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg 1261 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac 1321 ggatggcctt tttgcgtttc tacaaactct ttttgtttat ttttctaaat acattcaaat 1381 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag 1441 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt 1501 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt 1561 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc 1621 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta 1681 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac 1741 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa 1801 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg 1861 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc 1921 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg 1981 atgcctacag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta 2041 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg 2101 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg 2161 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc 2221 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt 2281 gcctcactga ttaagcattg gtaactgtca gaccaagttt acgagctcgc ttggactcct 2341 gttgatagat ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg 2401 ccgccgggcg ttttttattg gtgagaatcc aagcactagt catgaccaaa atcccttaac 2461 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 2521 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 2581 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 2641 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga 2701 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 2761 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 2821 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 2881 ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa 2941 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3001 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3061 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3121 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 3181 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca 3241 gccgaacgcc taggtctagg gcggcggatt tgtcctactc aggagagcgt tcaccgacaa 3301 acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct 3361 ctagcacgcg taccatggga tcccccgggc tgcaggaatt cgatatc //