LOCUS LC018349 6793 bp DNA circular SYN 10-OCT-2015 DEFINITION Expression vector pH_delta_NG DNA, complete sequence. ACCESSION LC018349 VERSION LC018349.1 KEYWORDS . SOURCE Expression vector pH_delta_NG ORGANISM Expression vector pH_delta_NG other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 6793) AUTHORS Kobayashi,H. TITLE Direct Submission JOURNAL Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases. Contact:Hideki Kobayashi Agency for Marine-Earth Science and Technology, Institute of Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL :http://www.jamstec.go.jp/j/ REFERENCE 2 AUTHORS Kobayashi,H. TITLE Inducible Suppression of Global Translation by Overuse of Rare Codons JOURNAL Appl. Environ. Microbiol. 81, 2544-2553 (2015) REMARK DOI:10.1128/AEM.03708-14 COMMENT FEATURES Location/Qualifiers source 1..6793 /db_xref="taxon:1602146" /host="E. coli" /mol_type="other DNA" /note="Coding hgfp_delta_1 and gfp" /organism="Expression vector pH_delta_NG" CDS complement(19..1134) /codon_start=1 /gene="lacI" /product="LacI" /protein_id="BAQ25588.1" /transl_table=11 /translation="MVYAKDGRMDVMKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAK TREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLG ASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLD VSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTR NQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVG ADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSL VKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ" CDS 1796..2332 /codon_start=1 /gene="hgfp_delta_1" /product="GFP deletion mutant" /protein_id="BAQ25589.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG IKVNFKIRHNIKDGSVQL" CDS 2860..3720 /codon_start=1 /gene="Amp" /product="beta-lactamase" /protein_id="BAQ25590.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3743..4665 /note="ColE1-derived plasmid replication origin" CDS complement(4741..5457) /codon_start=1 /gene="gfpmut3" /product="GFP" /protein_id="BAQ25591.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG IKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM ILLEFVTAAGITHGMDELYK" CDS 6107..6763 /codon_start=1 /gene="tetR" /product="Tet R repressor" /protein_id="BAQ25592.1" /transl_table=11 /translation="MVYAKDGRMDVMSRLDKSKVINSALELLNEVGIEGLTTRKLAQK LGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALL SHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQ EHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS " BASE COUNT 1751 a 1602 c 1688 g 1752 t ORIGIN 1 aagcttataa ggcgcgcctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 61 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt 121 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga 181 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt 241 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat 301 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg 361 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg 421 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt 481 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg 541 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg 601 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag 661 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag 721 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt 781 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc 841 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt 901 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat 961 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct 1021 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc 1081 gtataacgtt actggtttca tgacgtccat ccggccgtcc tttgcataca ccataggtgt 1141 ggtttaattt gatgcccttt ttcagggctg gaatgtgtaa gagcggggtt atttatgctg 1201 ttgttttttt gttactcggg aagggcttta cctcttccgc ataaacgctt ccatcagcgt 1261 ttatagttaa aaaaatcttt cggaactggt tttgcgctta ccccaaccaa caggggattt 1321 gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt ttgtgcatcc 1381 atctggattc tcctgtcagt tagctttggt ggtgtgtggc agttgtagtc ctgaacgaaa 1441 accccccgcg attggcacat tggcagctaa tccggaatcg cacttacggc caatgcttcg 1501 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat 1561 ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc attataaccg 1621 ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg gttatctgtg 1681 catgcattta cgttgacacc atcgaatggc tgaaatgagc tgttgacaat taatcatccg 1741 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa ccggtatgag 1801 caaaggcgaa gaactgttta ccggcgtggt gccgattctg gtggaactgg atggcgatgt 1861 gaacggccag aaatttagcg tgagcggcga aggcgaaggc gatgcgacct atggcaaact 1921 gaccctgaaa tttatttgca ccaccggcaa actgccggtg ccgtggccga ccctggtgac 1981 cacctttggc tatggcgtgc agtgctttgc gcgctatccg gatcatatga aacagcatga 2041 tttttttaaa agcgcgatgc cggaaggcta tgtgcaggaa cgcaccattt tttataaaga 2101 tgatggcaac tataaaaccc gcgcggaagt gaaatttgaa ggcgataccc tggtgaaccg 2161 cattgaactg aaaggcattg attttaaaga agatggcaac attctgggcc ataaaatgga 2221 atataactat aacagccata acgtgtatat tatggcggat aaaccgaaaa acggcattaa 2281 agtgaacttt aaaattcgcc ataacattaa agatggcagc gtgcagctgt aagctagctt 2341 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga 2401 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc 2461 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg 2521 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2581 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc 2641 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac 2701 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca 2761 aactcttttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 2821 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 2881 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 2941 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 3001 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 3061 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag 3121 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 3181 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 3241 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 3301 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 3361 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctacagcaat ggcaacaacg 3421 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 3481 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 3541 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 3601 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 3661 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 3721 ctgtcagacc aagtttacga gctcgcttgg actcctgttg atagatccag taatgacctc 3781 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 3841 gaatccaagc actagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 3901 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3961 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 4021 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 4081 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 4141 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 4201 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 4261 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 4321 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 4381 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 4441 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 4501 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 4561 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 4621 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgcctagc ttttatttgt 4681 atagttcatc catgccatgt gtaatcccag caggggctgc aggaattcga tatcaagctt 4741 ttatttgtat agttcatcca tgccatgtgt aatcccagca gctgttacaa actcaagaag 4801 gatcatgtga tctctctttt cgttgggatc tttggaaagg gcagattgtg tggacaggta 4861 atggttgtct ggtaaaagga cagggccatc gccaattgga gtattttgtt gataatggtc 4921 tgctaattga acgcttccat ctttaatgtt gtgtctaatt ttgaagttaa ctttgattcc 4981 attctttggt ttgtctgcca tgatgtatac attatgtgag ttatagttgt attccatttt 5041 gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga ttctattaac 5101 aagggtatca ccttcaaact tgacttcagc acgtgtcttg tagttcccgt catctttgta 5161 aaatatagtt ctttcctgta cataaccttc gggcatggca ctcttgaaaa agtcatgctg 5221 tttcatatga tctgggtatc tcgcaaagca ttgaacacca taaccgaaag tagtgacaag 5281 tgttggccat ggaacaggta gcttcccagt agtgcaaata aatttaaggg taagttttcc 5341 gtatgttgca tcaccttcac cctctccact gacagagaat ttttgcccat taacatcgcc 5401 atctaattca acaagaattg ggacaactcc agtgaaaagt tcttctcctt tactcatgac 5461 gtccatggtc tgtttcctcc tggtcagtgc gtcctgctga tgtgctcagt atctctatca 5521 ctgataggga tgtcaatctc tatcactgat agggagcatg cacagataac catctgcggt 5581 gataaattat ctctggcggt gttgacataa ataccactgg cggttataat gagcacatca 5641 gcaggacgca ctgaccacca tgaaggtgac gctcttaaaa attaagccct gaagaagggc 5701 agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gccgtaagtg 5761 cgattccgga ttagctgcca atgtgccaat cgcggggggt tttcgttcag gactacaact 5821 gccacacacc accaaagcta actgacagga gaatccagat ggatgcacaa acacgccgcc 5881 gcgaacgtcg cgcagagaaa caggctcaat ggaaagcagc aaatcccctg ttggttgggg 5941 taagcgcaaa accagttccg aaagattttt ttaactataa acgctgatgg aagcgtttat 6001 gcggaagagg taaagccctt cccgagtaac aaaaaaacaa cagcataaat aaccccgctc 6061 ttacacattc cagccctgaa aaagggcatc aaattaaacc acacctatgg tgtatgcaaa 6121 ggacggccgg atggacgtca tgtctagatt agataaaagt aaagtgatta acagcgcatt 6181 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct 6241 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc 6301 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag 6361 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg 6421 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga 6481 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact 6541 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc 6601 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat 6661 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat 6721 atgcggatta gaaaaacaac ttaaatgtga aagtgggtct taaggatccc ccgggctgca 6781 ggaattcgat atc //