LOCUS LC018346 8670 bp DNA circular SYN 10-OCT-2015 DEFINITION Expression vector pYLGFP1 DNA, complete sequence. ACCESSION LC018346 VERSION LC018346.1 KEYWORDS . SOURCE Expression vector pYLGFP1 ORGANISM Expression vector pYLGFP1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8670) AUTHORS Kobayashi,H. TITLE Direct Submission JOURNAL Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases. Contact:Hideki Kobayashi Agency for Marine-Earth Science and Technology, Institute of Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL :http://www.jamstec.go.jp/j/ REFERENCE 2 AUTHORS Kobayashi,H. TITLE Inducible Suppression of Global Translation by Overuse of Rare Codons JOURNAL Appl. Environ. Microbiol. 81, 2544-2553 (2015) REMARK DOI:10.1128/AEM.03708-14 COMMENT FEATURES Location/Qualifiers source 1..8670 /db_xref="taxon:1602143" /host="Yeasr" /mol_type="other DNA" /note="Coding galactose inducible lgfp" /organism="Expression vector pYLGFP1" CDS 257..1117 /codon_start=1 /gene="Amp" /product="beta-lactamase" /protein_id="BAQ25579.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" CDS 5255..5971 /codon_start=1 /gene="lgfp" /product="GFP" /protein_id="BAQ25580.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG IKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM ILLEFVTAAGITHGMDELYK" BASE COUNT 2446 a 1815 c 1847 g 2562 t ORIGIN 1 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 61 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 121 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 481 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 781 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga 841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 1681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2041 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 2101 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2161 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 2221 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 2281 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 2341 cagctgggcc attctcatga agaatatctt gaatttattg tcatattact agttggtgtg 2401 gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc 2461 ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac 2521 tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag 2581 tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc 2641 tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct 2701 taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt 2761 taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca 2821 tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat 2881 tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta 2941 ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata 3001 aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg 3061 gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca 3121 gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta 3181 tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc 3241 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa 3301 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat 3361 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc 3421 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag 3481 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac 3541 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat 3601 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt ttcaattcat 3661 catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt gattcggtaa 3721 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca 3781 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa 3841 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta 3901 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact 3961 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag 4021 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg 4081 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca 4141 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa 4201 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg 4261 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag 4321 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg 4381 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag 4441 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg 4501 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta 4561 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca 4621 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat 4681 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt 4741 tattacccac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct 4801 ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac 4861 tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg 4921 gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga 4981 taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt 5041 tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca 5101 acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt 5161 taatatacct ctatacttta acgtcaagga gaaaaaaccc cggatcggac tactagcagc 5221 tgtaatacga ctcactatag ggaatattaa gcttatgtca aagggggagg agctattcac 5281 aggggtagta cccatactag tagagctaga cggggacgta aatgggcaaa agttctcagt 5341 atcaggggag ggggaggggg acgctacata cgggaagcta acactaaagt tcatatgtac 5401 aacagggaag ctacccgtac cctggcccac actagtaaca acattcgggt acggggtaca 5461 atgtttcgct aggtaccccg accacatgaa gcaacacgac ttcttcaagt cagctatgcc 5521 cgaggggtac gtacaagaga ggacaatatt ctacaaggac gacgggaatt acaagacaag 5581 ggctgaggta aagttcgagg gggacacact agtaaatagg atagagctaa aggggataga 5641 cttcaaggag gacgggaata tactagggca caagatggag tacaattaca attcacacaa 5701 tgtatacata atggctgaca agcccaagaa tgggataaag gtaaatttca agataaggca 5761 caatataaag gacgggtcag tacaactagc tgaccactac caacaaaata cacccatagg 5821 ggacgggccc gtactactac ccgacaatca ctacctatca acacaatcag ctctatcaaa 5881 ggaccccaat gagaagaggg accacatgat actactagag ttcgtaacag ctgctgggat 5941 aacacacggg atggacgagc tatacaagta ggagctcgga tccactagta acggccgcca 6001 gtgtgctgga attctgcaga tatccatcac actggcggcc gctcgagcat gcatctagag 6061 ggccgcatca tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct 6121 ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 6181 gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaga 6241 cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 6301 aaggctttaa tttgccgtac cgagctcgaa tttcgagaaa gtgcccatca gtgttcatat 6361 ttgttacaaa gtggttttct gatttggtac tactgcagag gcgtattttt tgcttcagtt 6421 accatagcgt aagaacacta gcgacttttg ttcgtgaacc aacagagtag gatttctact 6481 gctacatctc ttaggtagtt ggttagtccg atcgctcact tttggttgtt gttaagtact 6541 tcataagttt atccttttcc tttttcacac tgagctactt tgggtatagc ttttggccca 6601 aggatctttg aattttctcc aaaagtactt tattttatat cctacaggtt gcggttttca 6661 tattttaaaa agcttttaat cattcctttg cgtatggcaa accctttttc gagatggttt 6721 ctatcagaga gacctccaaa ctgccatgta gccgatttag aaacaagttt agatccccat 6781 caaacgttgt tgaaggtgca aaaatacaaa cccgctttaa gcgactgggt gcattacatc 6841 ttcttgggat ccatcatgct gtttgtgttc attactaatc ccgcaccttg gatcttcaag 6901 atcctttttt attgtttctt gggcacttta ttcatcattc cagctacgtc acagtttttc 6961 ttcaatgcct tgcccatcct aacatgggtg gcgctgtatt tcacttcatc gtactttcca 7021 gatgaccgca ggcctcctat tactgtcaaa gtgttaccag cggtggaaac aattttatac 7081 ggcgacaatt taagtgatat tcttgcaaca tcgacgaatt cctttttgga cattttagca 7141 tggttaccgt acggactatt tcattatggg gccccatttg tcgttgctgc catcttattc 7201 gtatttggtc caccaactgt tttgcaaggt tatgcttttg catttggtta tatgaacctg 7261 tttggtgtta tcatgcaaaa tgtctttcca gccgctcccc catggtataa aattctctat 7321 ggattgcaat cagccaacta tgatatgcat ggctcgcctg gtggattagc tagaattgat 7381 aagctactcg gtattaatat gtatactaca tgtttttcaa attcctccgt cattttcggt 7441 gcttttcctt cactgcattc cgggtgtgct actatggaag ccctgttttt ctgttattgt 7501 tttccaaaat tgaagccctt gtttattgct tatgtttgct ggttatggtg gtcaactatg 7561 tatctgacac accattattt tgtagacctt atggcaggtt ctgtgctgtc atacgttatt 7621 ttccagtaca caaagtacac acatttacca attgtagata catctctttt ttgcagatgg 7681 tcatacactt caattgagaa atacgatata tcaaagagtg atccattggc tgcagattca 7741 aacgatatcg aaagtgtccc tttgtccaac ttggaacttg actttgatct taatatgact 7801 gatgaaccca gtgtaagccc ttcgttattt gatggatcta cttctgtttc tcgttcgtcc 7861 gccacgtcta taacgtcact aggtgtaaag agggcttaat gagtatttta tctgcaatta 7921 cggatacggt tggtcttatg tagatacata taaatatata tctttttctt tctttttctt 7981 agtcaggatt gtcgtttagc ataatataca tgtagtttat ttaatcacat accactgatt 8041 atctttagaa ttttataaat ttttgaaata aatgggtggc ttttaatggt gtctatgtta 8101 agtgaggctt ttagaatgct cttcctgctt tgtttattat atgtgtatga aagatatgta 8161 tgtatttaca tgtgtttgta gcgtccccag tcaaaacctg tgcgctatac ctaaatggat 8221 tgataatctt cattcactaa ttctaaaata gacttcttcc ccaaagaacg gtgtaacgat 8281 gaggctctat ccagctgctt atctaaatca actttaacga tggatgatct tatgacacgg 8341 ggatctttct ttaaagttct tagaatttca gactgtaccg cagctgatga atcaaacagc 8401 attaaaaagt gatatgctcg aaaatgtttt tcctggtctt tcttcattat tttaggaaga 8461 taccttatgc ccatgggtac aatgtccctc accacacctc tgttttgaat aatcagtttc 8521 ccgattgtgg aagacaattc ttttgcttcc aactttggcg cattggagtt ggttatgcga 8581 acaagtccga tcagctcata aagcatctta gtgaaaaggg tggttttgcg ttattctttc 8641 ctctggatcc tctagagtcg acctcgaggc //