LOCUS LC018344 5003 bp DNA circular SYN 10-OCT-2015 DEFINITION Expression vector pHGFP DNA, complete sequence. ACCESSION LC018344 VERSION LC018344.1 KEYWORDS . SOURCE Expression vector pHGFP ORGANISM Expression vector pHGFP other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5003) AUTHORS Kobayashi,H. TITLE Direct Submission JOURNAL Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases. Contact:Hideki Kobayashi Agency for Marine-Earth Science and Technology, Institute of Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL :http://www.jamstec.go.jp/j/ REFERENCE 2 AUTHORS Kobayashi,H. TITLE Inducible Suppression of Global Translation by Overuse of Rare Codons JOURNAL Appl. Environ. Microbiol. 81, 2544-2553 (2015) REMARK DOI:10.1128/AEM.03708-14 COMMENT FEATURES Location/Qualifiers source 1..5003 /db_xref="taxon:1602141" /host="E. coli" /mol_type="other DNA" /note="coding IPTG inducible hgfp" /organism="Expression vector pHGFP" CDS complement(19..1134) /codon_start=1 /gene="lacI" /product="LacI" /protein_id="BAQ25574.1" /transl_table=11 /translation="MVYAKDGRMDVMKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAK TREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLG ASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLD VSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTR NQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVG ADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSL VKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ" CDS 1796..2512 /codon_start=1 /gene="hgfp" /product="GFP" /protein_id="BAQ25575.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG IKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM ILLEFVTAAGITHGMDELYK" CDS 3040..3900 /codon_start=1 /gene="Amp" /product="beta-lactamase" /protein_id="BAQ25576.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3923..5003 /note="ColE1-derived plasmid replication origin" BASE COUNT 1216 a 1246 c 1311 g 1230 t ORIGIN 1 aagcttataa ggcgcgcctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 61 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt 121 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga 181 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt 241 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat 301 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg 361 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg 421 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt 481 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg 541 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg 601 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag 661 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag 721 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt 781 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc 841 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt 901 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat 961 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct 1021 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc 1081 gtataacgtt actggtttca tgacgtccat ccggccgtcc tttgcataca ccataggtgt 1141 ggtttaattt gatgcccttt ttcagggctg gaatgtgtaa gagcggggtt atttatgctg 1201 ttgttttttt gttactcggg aagggcttta cctcttccgc ataaacgctt ccatcagcgt 1261 ttatagttaa aaaaatcttt cggaactggt tttgcgctta ccccaaccaa caggggattt 1321 gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt ttgtgcatcc 1381 atctggattc tcctgtcagt tagctttggt ggtgtgtggc agttgtagtc ctgaacgaaa 1441 accccccgcg attggcacat tggcagctaa tccggaatcg cacttacggc caatgcttcg 1501 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat 1561 ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc attataaccg 1621 ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg gttatctgtg 1681 catgcattta cgttgacacc atcgaatggc tgaaatgagc tgttgacaat taatcatccg 1741 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa ccggtatgag 1801 caaaggcgaa gaactgttta ccggcgtggt gccgattctg gtggaactgg atggcgatgt 1861 gaacggccag aaatttagcg tgagcggcga aggcgaaggc gatgcgacct atggcaaact 1921 gaccctgaaa tttatttgca ccaccggcaa actgccggtg ccgtggccga ccctggtgac 1981 cacctttggc tatggcgtgc agtgctttgc gcgctatccg gatcatatga aacagcatga 2041 tttttttaaa agcgcgatgc cggaaggcta tgtgcaggaa cgcaccattt tttataaaga 2101 tgatggcaac tataaaaccc gcgcggaagt gaaatttgaa ggcgataccc tggtgaaccg 2161 cattgaactg aaaggcattg attttaaaga agatggcaac attctgggcc ataaaatgga 2221 atataactat aacagccata acgtgtatat tatggcggat aaaccgaaaa acggcattaa 2281 agtgaacttt aaaattcgcc ataacattaa agatggcagc gtgcagctgg cggatcatta 2341 tcagcagaac accccgattg gcgatggccc ggtgctgctg ccggataacc attatctgag 2401 cacccagagc gcgctgagca aagatccgaa cgaaaaacgc gatcatatga ttctgctgga 2461 atttgtgacc gcggcgggca ttacccatgg catggatgaa ctgtataaat aagctagctt 2521 ggctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga 2581 agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc 2641 atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg 2701 agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 2761 tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc 2821 ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac 2881 tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca 2941 aactcttttt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 3001 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 3061 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 3121 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 3181 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 3241 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag 3301 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 3361 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 3421 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 3481 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 3541 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctacagcaat ggcaacaacg 3601 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 3661 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 3721 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 3781 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 3841 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 3901 ctgtcagacc aagtttacga gctcgcttgg actcctgttg atagatccag taatgacctc 3961 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga 4021 gaatccaagc actagtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 4081 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 4141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 4201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 4261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 4321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 4381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 4441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 4501 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 4561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 4621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 4681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 4741 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 4801 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgcctagg tctagggcgg 4861 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca 4921 gtctttcgac tgagcctttc gttttatttg atgcctctag cacgcgtacc atgggatccc 4981 ccgggctgca ggaattcgat atc //