LOCUS LC018341 4466 bp DNA circular SYN 10-OCT-2015 DEFINITION Expression vector pLGFP_delta_3 DNA, complete sequence. ACCESSION LC018341 VERSION LC018341.1 KEYWORDS . SOURCE Expression vector pLGFP_delta_3 ORGANISM Expression vector pLGFP_delta_3 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4466) AUTHORS Kobayashi,H. TITLE Direct Submission JOURNAL Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases. Contact:Hideki Kobayashi Agency for Marine-Earth Science and Technology, Institute of Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL :http://www.jamstec.go.jp/j/ REFERENCE 2 AUTHORS Kobayashi,H. TITLE Inducible Suppression of Global Translation by Overuse of Rare Codons JOURNAL Appl. Environ. Microbiol. 81, 2544-2553 (2015) REMARK DOI:10.1128/AEM.03708-14 COMMENT FEATURES Location/Qualifiers source 1..4466 /db_xref="taxon:1602138" /mol_type="other DNA" /note="coding IPTG inducible lgfp_delta_3" /organism="Expression vector pLGFP_delta_3" CDS complement(19..1134) /codon_start=1 /gene="lacI" /product="LacI" /protein_id="BAQ25565.1" /transl_table=11 /translation="MVYAKDGRMDVMKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAK TREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLG ASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLD VSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTR NQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVG ADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSL VKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ" CDS 1796..1975 /codon_start=1 /gene="lgfp_delta_3" /product="GFP deletion mutant" /protein_id="BAQ25566.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPT" CDS 2503..3363 /codon_start=1 /gene="Amp" /product="beta-lactamase" /protein_id="BAQ25567.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3386..4466 /note="ColE1-derived plasmid replication origin" BASE COUNT 1060 a 1127 c 1176 g 1103 t ORIGIN 1 aagcttataa ggcgcgcctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 61 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt 121 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga 181 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt 241 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat 301 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg 361 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg 421 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt 481 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg 541 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg 601 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag 661 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag 721 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt 781 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc 841 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt 901 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat 961 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct 1021 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc 1081 gtataacgtt actggtttca tgacgtccat ccggccgtcc tttgcataca ccataggtgt 1141 ggtttaattt gatgcccttt ttcagggctg gaatgtgtaa gagcggggtt atttatgctg 1201 ttgttttttt gttactcggg aagggcttta cctcttccgc ataaacgctt ccatcagcgt 1261 ttatagttaa aaaaatcttt cggaactggt tttgcgctta ccccaaccaa caggggattt 1321 gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt ttgtgcatcc 1381 atctggattc tcctgtcagt tagctttggt ggtgtgtggc agttgtagtc ctgaacgaaa 1441 accccccgcg attggcacat tggcagctaa tccggaatcg cacttacggc caatgcttcg 1501 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat 1561 ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc attataaccg 1621 ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg gttatctgtg 1681 catgcattta cgttgacacc atcgaatggc tgaaatgagc tgttgacaat taatcatccg 1741 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa ccggtatgtc 1801 aaagggggag gagctattca caggggtagt acccatacta gtagagctag acggggacgt 1861 aaatgggcaa aagttctcag tatcagggga gggggagggg gacgctacat acgggaagct 1921 aacactaaag ttcatatgta caacagggaa gctacccgta ccctggccca cataggctag 1981 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc 2041 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac 2101 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat 2161 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2221 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg 2281 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata 2341 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct 2401 acaaactctt tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 2461 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 2521 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 2581 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 2641 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 2701 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa 2761 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 2821 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 2881 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 2941 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 3001 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctacagc aatggcaaca 3061 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 3121 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 3181 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 3241 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 3301 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 3361 taactgtcag accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac 3421 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 3481 tgagaatcca agcactagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 3721 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 3781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 3841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 3901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 3961 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4201 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4261 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgcct aggtctaggg 4321 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc 4381 ccagtctttc gactgagcct ttcgttttat ttgatgcctc tagcacgcgt accatgggat 4441 cccccgggct gcaggaattc gatatc //