LOCUS LC018340 4646 bp DNA circular SYN 10-OCT-2015 DEFINITION Expression vector pLGFP_delta_2 DNA, complete sequence. ACCESSION LC018340 VERSION LC018340.1 KEYWORDS . SOURCE Expression vector pLGFP_delta_2 ORGANISM Expression vector pLGFP_delta_2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4646) AUTHORS Kobayashi,H. TITLE Direct Submission JOURNAL Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases. Contact:Hideki Kobayashi Agency for Marine-Earth Science and Technology, Institute of Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan URL :http://www.jamstec.go.jp/j/ REFERENCE 2 AUTHORS Kobayashi,H. TITLE Inducible Suppression of Global Translation by Overuse of Rare Codons JOURNAL Appl. Environ. Microbiol. 81, 2544-2553 (2015) REMARK DOI:10.1128/AEM.03708-14 COMMENT FEATURES Location/Qualifiers source 1..4646 /db_xref="taxon:1602137" /mol_type="other DNA" /note="coding IPTG inducible lgfp_delta_2" /organism="Expression vector pLGFP_delta_2" CDS complement(19..1134) /codon_start=1 /gene="lacI" /product="LacI" /protein_id="BAQ25562.1" /transl_table=11 /translation="MVYAKDGRMDVMKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAK TREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLG ASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLD VSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTR NQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVG ADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSL VKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ" CDS 1796..2155 /codon_start=1 /gene="lgfp_delta_2" /product="GFP deletion mutant" /protein_id="BAQ25563.1" /transl_table=11 /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD DGNYKTRAEVKFEGDTL" CDS 2683..3543 /codon_start=1 /gene="Amp" /product="beta-lactamase" /protein_id="BAQ25564.1" /transl_table=11 /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPTAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 3566..4646 /note="ColE1-derived plasmid replication origin" BASE COUNT 1118 a 1167 c 1225 g 1136 t ORIGIN 1 aagcttataa ggcgcgcctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 61 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt 121 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga 181 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt 241 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat 301 atccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg 361 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg 421 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt 481 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg 541 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg 601 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag 661 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag 721 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt 781 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc 841 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt 901 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat 961 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct 1021 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc 1081 gtataacgtt actggtttca tgacgtccat ccggccgtcc tttgcataca ccataggtgt 1141 ggtttaattt gatgcccttt ttcagggctg gaatgtgtaa gagcggggtt atttatgctg 1201 ttgttttttt gttactcggg aagggcttta cctcttccgc ataaacgctt ccatcagcgt 1261 ttatagttaa aaaaatcttt cggaactggt tttgcgctta ccccaaccaa caggggattt 1321 gctgctttcc attgagcctg tttctctgcg cgacgttcgc ggcggcgtgt ttgtgcatcc 1381 atctggattc tcctgtcagt tagctttggt ggtgtgtggc agttgtagtc ctgaacgaaa 1441 accccccgcg attggcacat tggcagctaa tccggaatcg cacttacggc caatgcttcg 1501 tttcgtatca cacaccccaa agccttctgc tttgaatgct gcccttcttc agggcttaat 1561 ttttaagagc gtcaccttca tggtggtcag tgcgtcctgc tgatgtgctc attataaccg 1621 ccagtggtat ttatgtcaac accgccagag ataatttatc accgcagatg gttatctgtg 1681 catgcattta cgttgacacc atcgaatggc tgaaatgagc tgttgacaat taatcatccg 1741 gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa ccggtatgtc 1801 aaagggggag gagctattca caggggtagt acccatacta gtagagctag acggggacgt 1861 aaatgggcaa aagttctcag tatcagggga gggggagggg gacgctacat acgggaagct 1921 aacactaaag ttcatatgta caacagggaa gctacccgta ccctggccca cactagtaac 1981 aacattcggg tacggggtac aatgtttcgc taggtacccc gaccacatga agcaacacga 2041 cttcttcaag tcagctatgc ccgaggggta cgtacaagag aggacaatat tctacaagga 2101 cgacgggaat tacaagacaa gggctgaggt aaagttcgag ggggacacac tataggctag 2161 cttggctgtt ttggcggatg agagaagatt ttcagcctga tacagattaa atcagaacgc 2221 agaagcggtc tgataaaaca gaatttgcct ggcggcagta gcgcggtggt cccacctgac 2281 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gtctccccat 2341 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2401 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg 2461 agcggatttg aacgttgcga agcaacggcc cggagggtgg cgggcaggac gcccgccata 2521 aactgccagg catcaaatta agcagaaggc catcctgacg gatggccttt ttgcgtttct 2581 acaaactctt tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 2641 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 2701 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 2761 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 2821 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 2881 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa 2941 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 3001 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 3061 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 3121 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 3181 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctacagc aatggcaaca 3241 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 3301 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 3361 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 3421 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 3481 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 3541 taactgtcag accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac 3601 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg 3661 tgagaatcca agcactagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3721 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3781 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3841 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 3901 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 3961 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 4021 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 4081 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 4141 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 4201 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4261 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4321 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4381 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4441 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgcct aggtctaggg 4501 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc 4561 ccagtctttc gactgagcct ttcgttttat ttgatgcctc tagcacgcgt accatgggat 4621 cccccgggct gcaggaattc gatatc //