LOCUS       LC018337                 720 bp    DNA     linear   SYN 10-OCT-2015
DEFINITION  Synthetic construct hu-lgfp gene for green fluorescent protein,
            complete cds.
ACCESSION   LC018337
VERSION     LC018337.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Kobayashi,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases.
            Contact:Hideki Kobayashi
            Agency for Marine-Earth Science and Technology, Institute of
            Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan
            URL    :http://www.jamstec.go.jp/j/
REFERENCE   2  
  AUTHORS   Kobayashi,H.
  TITLE     Inducible Suppression of Global Translation by Overuse of Rare
            Codons
  JOURNAL   Appl. Environ. Microbiol. 81, 2544-2553 (2015)
  REMARK    DOI:10.1128/AEM.03708-14
COMMENT     
FEATURES             Location/Qualifiers
     source          1..720
                     /db_xref="taxon:32630"
                     /mol_type="other DNA"
                     /note="derived from gfp with changing codon to lowest
                     usage"
                     /organism="synthetic construct"
     CDS             1..720
                     /codon_start=1
                     /gene="hu-lgfp"
                     /product="green fluorescent protein"
                     /protein_id="BAQ25555.1"
                     /transl_table=11
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFE
                     DDGNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MIYFGFVTAAAITHGMDELYK"
BASE COUNT          252 a           98 c          168 g          202 t
ORIGIN      
        1 atggtatcga aaggtgcgga actatttacg ggtatagtac cgatactaat agaactaaat
       61 ggtgatgtaa atggtcataa attttcggta tcgggtgaag gtgaaggtga tgcgacgtat
      121 ggtaaactaa cgctaaaatt tatatgtacg acgggtaaac taccggtacc gtggccgacg
      181 ctagtaacga cgctatcgta tggtgtacaa tgtttttcgc gttatccgga tcatatgaaa
      241 caacatgatt tttttaaatc ggcgatgccg gaaggttata tacaagaacg tacgatattt
      301 tttgaagatg atggtaatta taaatcgcgt gcggaagtaa aatttgaagg tgatacgcta
      361 gtaaatcgta tagaactaac gggtacggat tttaaagaag atggtaatat actaggtaat
      421 aaaatggaat ataattataa tgcgcataat gtatatataa tgacggataa agcgaaaaat
      481 ggtataaaag taaattttaa aatacgtcat aatatagaag atggttcggt acaactagcg
      541 gatcattatc aacaaaatac gccgataggt gatggtccgg tactactacc ggataatcat
      601 tatctatcga cgcaatcggc gctatcgaaa gatccgaatg aaaaacgtga tcatatgata
      661 tattttggtt ttgtaacggc ggcggcgata acgcatggta tggatgaact atataaatag
//