LOCUS       LC018335                 717 bp    DNA     linear   SYN 10-OCT-2015
DEFINITION  Synthetic construct hgfp gene for green fluorescent protein,
            complete cds.
ACCESSION   LC018335
VERSION     LC018335.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Kobayashi,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases.
            Contact:Hideki Kobayashi
            Agency for Marine-Earth Science and Technology, Institute of
            Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan
            URL    :http://www.jamstec.go.jp/j/
REFERENCE   2  
  AUTHORS   Kobayashi,H.
  TITLE     Inducible Suppression of Global Translation by Overuse of Rare
            Codons
  JOURNAL   Appl. Environ. Microbiol. 81, 2544-2553 (2015)
  REMARK    DOI:10.1128/AEM.03708-14
COMMENT     
FEATURES             Location/Qualifiers
     source          1..717
                     /db_xref="taxon:32630"
                     /mol_type="other DNA"
                     /note="derived from gfpmut3 with changing codon to
                     optimum codons"
                     /organism="synthetic construct"
     CDS             1..717
                     /codon_start=1
                     /gene="hgfp"
                     /product="green fluorescent protein"
                     /protein_id="BAQ25553.1"
                     /transl_table=11
                     /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG
                     IKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     ILLEFVTAAGITHGMDELYK"
BASE COUNT          212 a          155 c          192 g          158 t
ORIGIN      
        1 atgagcaaag gcgaagaact gtttaccggc gtggtgccga ttctggtgga actggatggc
       61 gatgtgaacg gccagaaatt tagcgtgagc ggcgaaggcg aaggcgatgc gacctatggc
      121 aaactgaccc tgaaatttat ttgcaccacc ggcaaactgc cggtgccgtg gccgaccctg
      181 gtgaccacct ttggctatgg cgtgcagtgc tttgcgcgct atccggatca tatgaaacag
      241 catgattttt ttaaaagcgc gatgccggaa ggctatgtgc aggaacgcac cattttttat
      301 aaagatgatg gcaactataa aacccgcgcg gaagtgaaat ttgaaggcga taccctggtg
      361 aaccgcattg aactgaaagg cattgatttt aaagaagatg gcaacattct gggccataaa
      421 atggaatata actataacag ccataacgtg tatattatgg cggataaacc gaaaaacggc
      481 attaaagtga actttaaaat tcgccataac attaaagatg gcagcgtgca gctggcggat
      541 cattatcagc agaacacccc gattggcgat ggcccggtgc tgctgccgga taaccattat
      601 ctgagcaccc agagcgcgct gagcaaagat ccgaacgaaa aacgcgatca tatgattctg
      661 ctggaatttg tgaccgcggc gggcattacc catggcatgg atgaactgta taaataa
//