LOCUS       LC018331                 717 bp    DNA     linear   SYN 10-OCT-2015
DEFINITION  Synthetic construct lgfp gene for green fluorescent protein,
            complete cds.
ACCESSION   LC018331
VERSION     LC018331.1
KEYWORDS    .
SOURCE      synthetic construct
  ORGANISM  synthetic construct
            other sequences; artificial sequences.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Kobayashi,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2015) to the DDBJ/EMBL/GenBank databases.
            Contact:Hideki Kobayashi
            Agency for Marine-Earth Science and Technology, Institute of
            Biogeosciences; 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan
            URL    :http://www.jamstec.go.jp/j/
REFERENCE   2  
  AUTHORS   Kobayashi,H.
  TITLE     Inducible Suppression of Global Translation by Overuse of Rare
            Codons
  JOURNAL   Appl. Environ. Microbiol. 81, 2544-2553 (2015)
  REMARK    DOI:10.1128/AEM.03708-14
COMMENT     
FEATURES             Location/Qualifiers
     source          1..717
                     /db_xref="taxon:32630"
                     /mol_type="other DNA"
                     /note="derived from gfpmut3, with changing codon to
                     lowest usage"
                     /organism="synthetic construct"
     CDS             1..717
                     /codon_start=1
                     /gene="lgfp"
                     /product="green fluorescent protein"
                     /protein_id="BAQ25549.1"
                     /transl_table=11
                     /translation="MSKGEELFTGVVPILVELDGDVNGQKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFYKD
                     DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKMEYNYNSHNVYIMADKPKNG
                     IKVNFKIRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     ILLEFVTAAGITHGMDELYK"
BASE COUNT          251 a          152 c          187 g          127 t
ORIGIN      
        1 atgtcaaagg gggaggagct attcacaggg gtagtaccca tactagtaga gctagacggg
       61 gacgtaaatg ggcaaaagtt ctcagtatca ggggaggggg agggggacgc tacatacggg
      121 aagctaacac taaagttcat atgtacaaca gggaagctac ccgtaccctg gcccacacta
      181 gtaacaacat tcgggtacgg ggtacaatgt ttcgctaggt accccgacca catgaagcaa
      241 cacgacttct tcaagtcagc tatgcccgag gggtacgtac aagagaggac aatattctac
      301 aaggacgacg ggaattacaa gacaagggct gaggtaaagt tcgaggggga cacactagta
      361 aataggatag agctaaaggg gatagacttc aaggaggacg ggaatatact agggcacaag
      421 atggagtaca attacaattc acacaatgta tacataatgg ctgacaagcc caagaatggg
      481 ataaaggtaa atttcaagat aaggcacaat ataaaggacg ggtcagtaca actagctgac
      541 cactaccaac aaaatacacc cataggggac gggcccgtac tactacccga caatcactac
      601 ctatcaacac aatcagctct atcaaaggac cccaatgaga agagggacca catgatacta
      661 ctagagttcg taacagctgc tgggataaca cacgggatgg acgagctata caagtag
//