LOCUS SYNPE194V 3728 bp DNA circular SYN 26-JUL-1993
DEFINITION pE194 cloning vector.
ACCESSION L08862
VERSION L08862.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3728)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
COMMENT These data and their annotation were supplied to GenBank by Will
Gilbert under the auspices of the GenBank Currator Program. pE194 -
Cloning Vector
ENTRY PE194 #TYPE DNA CIRCULAR
TITLE pE194 - Cloning Vector
DATE 11-JUN-1987
ACCESSION VB0131
SOURCE Staphylococcus aureus
COLLECTION ATCC 37128
REFERENCE
#number 1
#authors Gryczan T.J., Grandi G., Hahn J., Grandi R., Dubnau
D.
#journal Nucl. Acids Res. (1980) 8: 6081-6097
#title
Conformational alteration of mRNA structure and the
posttranscriptional
regulation of erythromycin-induced drug resistance
#comment (bases 1722 to 3074; complementary strand) REFERENCE
#number 2
#authors Horinouchi S., Weisblum B.
#journal Proc. Nat. Acad. Sci. USA (1980) 77: 7079-7083
#title
Posttranscriptional modification of mRNA conformation:
Mechanism that
regulates erythromycin-induced resistance
#comment (bases 1940 to 3386; complementary strand) REFERENCE
#number 3
#authors Horinouchi S., Weisblum B.
#journal Mol. Gen. Genet. (1981) 182: 341-348
#title
The control region for erythromycin resistance: Free energy
changes
related to induction and mutation of constitutive expression
#comment (bases 2629 to 2927; complementary strand) REFERENCE
#number 4
#authors Horinouchi S., Weisblum B.
#journal J. Bacteriol. (1982) 150: 804-814
#title
Nucleotide sequence and functional map of pE194, a plasmid
that specifies
inducible resistance to macrolide, lincosamide, and
streptogramin type B
antibiotics
#comment (bases 1 to 3727)
REFERENCE
#number 5
#authors Hahn J., Grandi G., Gryczan T.J., Dubnau D.
#journal Mol. Gen. Genet. (1982) 186: 204-216
#title
Translational attenuation of ermC: A deletion analysis
#comment (bases 2858 to 3335; complementary strand) REFERENCE
#number 6
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector IV-A-a-i-2
COMMENT
fetched from GenBank(48)
COMMENT
this natural isolate from Staphylococcus aureus has been used as
cloning
vector in Bacillus subtilis and other gram-positive species.
COMMENT from GenBank(48)
[4] found five open reading frames, three of which (E, 1174-857;
D,
1244-1594; and C, 1506-1859) lacked putative promoter regions.
[3] proposes that the control region of the erythromycin
resistance gene,
located at the 5' end of the messenger, is transcribed along
with the
coding sequence. This region comprises a series of inverted
complementary
repeats that can redistribute and assume alternate
double-stranded
conformations. Activation of the messenger depends on the degree
to which
ribosomes, stalled by erythromycin, disrupt the secondary
structure in a
part of the control region that simultaneously codes for a small
peptide
and overlaps two of the inverted repeats. The result of the
inverted
repeat redistribution is the unmasking of a ribosome binding
site for
synthesis of the protein that mediates the resistance phenotype.
[5] constructed deletants missing the ermC promoter and parts of
the leader region. The ermC transcription was dependent on
fusion
to an upstream promoter. As the deletions extended deeper into
the
leader region, successively masking and unmasking sequences
required for translation of the methylase, an alternation of
high
and low level methylase expression was observed.
FEATURES from to/span description
pept 2738 2004 (c) adenine methylase (ermC)
pept 2857 2798 (c) leader peptide
SITES
conflict 1871 3 agg in [4]; aagag in [1]
->pept 2004 1 (c) ermC cds end
pept<- 2738 1 (c) ermC cds start
->pept 2798 1 (c) leader pept cds end [1]
pept<- 2857 1 (c) leader pept cds start [1]
mRNA<- 2877 1 (c) ermC mRNA start [1] KEYWORDS
adenine methylase \ erythromycin resistance \ leader peptide \
lincosamide resistance \ macrolide resistance \ streptogramin
resistance
CROSSREFERENCE
#identical
GenBank(48):PE1
PARENT
Features of pE194 (3728 bp)
original isolate, no homology to other sequences FEATURE
2004-2738 735-1 (c) erythromycin resistance protein (ermC)
SELECTION
#resistance erythromycin
SUMMARY pE194 #length 3728 #checksum 1540.
FEATURES Location/Qualifiers
source 1..3728
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
BASE COUNT 1363 a 460 c 721 g 1184 t
ORIGIN
1 gatcagtaca agaaagatac tgtatttcat aaacaggaac tgcaagaagt taaggatgag
61 ttacagaagg caaataagca gttacagagt ggaatagagc atatgaggtc tacgaaaccc
121 tttgattatg aaaatgagcg tacaggtttg ttctctggac gtgaagagac tggtagaaag
181 atattaactg ctgatgaatt tgaacgcctg caagaaacaa tctcttctgc agaacggatt
241 gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa
301 aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa
361 agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta
421 tcagagaaat ttcaagctag tacagtgact ttatatcgtg ctgcgagggc gaatttccct
481 gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc caaatttgag
541 cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt
601 gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt
661 tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat
721 taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag
781 gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt
841 tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc
901 taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa
961 ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atagttgtag
1021 atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga
1081 tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa
1141 taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact
1201 ggcatgctgc ctatctatga ctaaattttg ttaagtgtat tagcaccgtt attatatcat
1261 gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac
1321 atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca
1381 cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag gtaggatgaa
1441 aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa
1501 aataattaac agaagaattg aatgcgacta ttccgcagat tgcaggaagt gtgaaaggtc
1561 ttgtgagata tatgcttcac atggacgatc ctaataaatt taaatatcaa aaagaagata
1621 tgatagttta tggcggtgta gatgttgatg aattattaaa gaaaacaaca acagatagat
1681 ataaattaat taaagaaatg attgagttta ttgatgaaca aggaatcgta gaatttaaga
1741 gtttaatgga ttatgcaatg aagtttaaat ttgatgattg gttcccgctt ttatgtgata
1801 actcggcgta tgttattcaa gaatatataa aatcaaatcg gtataaatct gaccgataga
1861 ttttgaattt aggtgtcaca agacactctt ttttcgcacc agcgaaaact ggtttaagcc
1921 gactgcgcaa aagacataat cgattcacaa aaaataggca cacgaaaaac aagttaaggg
1981 atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
2041 aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
2101 ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
2161 taattatact tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata
2221 agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
2281 gaaatatcaa cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt
2341 ttagcaaacc cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt
2401 ttgcgtatta tatccgtact tatgttataa ggtatattac catatatttt ataggattgg
2461 tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
2521 acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
2581 ttacacctct ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata
2641 ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
2701 ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt
2761 atatgaattt tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat
2821 gaactgtgct gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta
2881 gtataattat agcacgagct ctgataaata tgaacatgat gagtgatcgt taaatttata
2941 ctgcaatcgg atgcgattat tgaataaaag atatgagaga tttatctaat ttcttttttc
3001 ttgtaaaaaa agaaagttct taaaggtttt atagttttgg tcgtagagca cacggtttaa
3061 cgacttaatt acgaagtaaa taagtctagt gtgttagact ttatgaaatc tatatacgtt
3121 tatatatatt tattatccgg aggtgtagca tgtctcattc aattttgagg gttgccagag
3181 ttaaaggatc aagtaataca aacgggatac aaagacataa tcaaagagag aataaaaact
3241 ataataataa agacataaat catgaggaaa catataaaaa ttatgatttg attaacgcac
3301 aaaatataaa gtataaagat aaaattgatg aaacgattga tgagaattat tcagggaaac
3361 gtaaaattcg gtcagatgca attcgacatg tggacggact ggttacaagt gataaagatt
3421 tctttgatga tttaagcgga gaagaaatag aacgattttt taaagatagc ttggagtttc
3481 tagaaaatga atacggtaag gaaaatatgc tgtatgcgac tgtccatctg gatgaaagag
3541 tcccacatat gcactttggt tttgtccctt taacagagga cgggagattg tctgcaaaag
3601 aacagttagg caacaagaaa gactttactc aattacaaga tagatttaat gagtatgtga
3661 atgagaaagg ttatgaactt gaaagaggca cgtccaaaga ggttacagaa cgagaacata
3721 aagcgatg
//