LOCUS SYNPBR325V 5996 bp DNA circular SYN 26-JUL-1993
DEFINITION pBR325 cloning vector.
ACCESSION L08855
VERSION L08855.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 5996)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
COMMENT These data and their annotation were supplied to GenBank by Will
Gilbert under the auspices of the GenBank Currator Program. pBR325
- Cloning Vector
ENTRY PBR325 #TYPE DNA CIRCULAR TITLE pBR325 -
Cloning Vector
DATE 12-SEP-1986
#sequence 16-DEC-1986
ACCESSION VB0002
SOURCE artificial
REFERENCE
#number 1
#authors Prentki P., Karch F., Iida S., Meyer J.
#journal Gene (1981) 14: 289-299
#comment pBR325, residues 4314-4419 and 5402-34 REFERENCE
#number 2
#authors Alton N.K., Vapnek D.
#journal Nature (1979) 282: 864-869
#comment Transposon Tn9, residues 4358-5444
This sequence differs from that shown in having 5150-C.
REFERENCE
#number 3
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector I-A-iv-4
COMMENT
Revised 16-DEC-1986 by F. Pfeiffer:
2731/2 'AT' to 'TA' to match revised sequence of PBR322 COMMENT
There is a sequence conflict in that pMAC58 contains a TTG
sequence where
its parent pBR325 at pos 5264-5265 only contains a TG. It is
unclear, if
this is a mutation in pMAC58, or if the sequence of pBR325 is
incorrect at
that position.
COMMENT
The plasmid pBR325 is a cloning vector constructed in vitro by
the addition of the chloramphenicol resistance gene of an
IS1-flanked transposon to pBR322. Sequence analysis suggests
that this transposon is related to transposons Tn9 and Tn981
but is longer. No portion of insertion sequence IS1 was found
in the genome.
COMMENT
This sequence was reconstructed from the genome of pBR322,
transposons Tn9 and Tn981, and pBR325 genome fragments. The
actual pBR325 and pBR328 genomes may differ from this
sequence.
COMMENT
In pMAC58, a pBR325-derived vector, a TTG sequence was found
where pBR325
has only a TG (pos 5264-65). It is not known if this discrepancy
is a
mutation or a sequence error in pBR325.
COMMENT
1-5996/Genome of pBR325
4358-5444/Transposon Tn9
4358-5263/Transposon Tn981
86-1273/Protein: Tetracycline resistance protein
1917-2105/Protein: Regulatory protein rop
2271(C)/cAMP-dependent pBR-P4 transcription initiation site
4155-3298(C)/Protein: Penicillinase (EC 3.5.2.6) precursor
4155-4087(C)/Signal sequence
4086-3298(C)/Penicillinase
4566-5222/Protein: Chloramphenicol acetyltransferase (EC
2.3.1.28)
KEYWORDS
CROSSREFERENCE
#prerevised
PIRN(30):pBR325
#parent
VecBase(3):pBR322, GenBank(50):Trn9Cat
#offspring
VecBase(3):pBR328, VecBase(3):pBR329,
VecBase(3):pJSC73, VecBase(3):pMAC58, VecBase(3):pMAC5bg14
VecBase(3):pSV2Cat, VecBase(3):YRp17, VecBase(3):pAA37X PARENT
Features of pBR325 (5996 bp)
residue source
1- 5 834- 830 (c) pBR322
5-4358 5-4358 pBR322
4358-5444 36-1122 Tn9
5520-5996 1311- 835 (c) pBR322
Conflict (cfl) and Mutations (mut):
pBR325 source
mut 5150 T C 828 Tn9
FEATURE
residue source
86-1276 1-1191 Tc-R; tetracycline resistance protein
3298-4086 789- 1 (c) Ap-R; b-lactamase
4566-5225 1- 660 Cm-R; chloramphenicol acetyltransferase
POLYLINKER none
SELECTION #resistance Ap, Tc, Cm
SUMMARY pBR325 #length 5996 #checksum 4248.
FEATURES Location/Qualifiers
source 1..5996
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
BASE COUNT 1395 a 1600 c 1565 g 1436 t
ORIGIN
1 aggccatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
1861 ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg catcaagtga
1921 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
1981 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc
2041 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa
2101 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
2161 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
2221 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
2281 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
2341 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
2401 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
2461 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
2521 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
2581 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
2641 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
2701 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
2761 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
2821 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
2881 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
2941 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
3001 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3061 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3121 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3181 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3241 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
3301 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
3361 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
3421 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
3481 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
3541 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
3601 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
3661 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
3721 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
3781 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
3841 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
3901 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
3961 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4021 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4081 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4141 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4201 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4261 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
4321 ctataaaaat aggcgtatca cgaggccctt tcgtcttcga ataaatacct gtgacggaag
4381 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
4441 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
4501 aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag
4561 ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta
4621 aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc
4681 tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct
4741 ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg gcaatgaaag
4801 acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc catgagcaaa
4861 ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag tttctacaca
4921 tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct aaagggttta
4981 ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt tttgatttaa
5041 acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa tattatacgc
5101 aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt tgtgatggct
5161 tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg cagggcgggg
5221 cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc tggtgctacg cctgaataag
5281 tgataataag cggatgaatg gcagaaattc gaaagcaaat tcgacccggt cgtcggttca
5341 gggcagggtc gttaaatagc cgcttatgtc tattgctggt ttaccggttt attgactacc
5401 ggaagcagtg tgaccgtgtg cttctcaaat gcctgaggcc agtttgctca ggctctcccc
5461 gtggaggtaa taattgacga tatgatcatt tattctgcct cccagagcct gataaaaacg
5521 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
5581 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
5641 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
5701 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
5761 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
5821 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
5881 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
5941 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgac
//