LOCUS KV891522 368823 bp DNA linear CON 24-FEB-2017
DEFINITION Opisthorchis viverrini isolate Khon Kaen unplaced genomic scaffold
O_viverrini-1.0_Cont67, whole genome shotgun sequence.
ACCESSION KV891522 LASN01000000
VERSION KV891522.1
DBLINK BioProject: PRJNA230518
BioSample: SAMN03378119
KEYWORDS WGS; HIGH_QUALITY_DRAFT.
SOURCE Opisthorchis viverrini
ORGANISM Opisthorchis viverrini
Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
Trematoda; Digenea; Opisthorchiida; Opisthorchiata;
Opisthorchiidae; Opisthorchis.
REFERENCE 1 (bases 1 to 368823)
AUTHORS Mitreva,M.
TITLE Draft genome of the nematode, Opisthorchis viverrini
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 368823)
AUTHORS Mitreva,M., Pepin,K.H., Martin,J., Ozersky,P., Palsikar,V.B.,
Zhang,X. and Wilson,R.K.
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2015) The Genome Institute, Washington University
School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA
COMMENT The human liver flukes, Opisthorchis viverrini, O. felineus and C.
sinensis remain important public health problems in many parts of
the world. Clonorchis sinensis is widespread in China, Korea and
Vietnam, while O. viverrini is endemic in Southeast Asia, including
Thailand, Lao Peoples Democratic Republic (Lao PDR), Cambodia and
central Vietnam. Human infection follows the consumption of raw or
undercooked cyprinoid (freshwater) fish haboring infective
metacercariae. Recent reports suggested that about 35 million
people are infected with C. sinensis globally; with up to 15
million human infections in China alone and another 8-10 million
individuals infected with O. viverrini in Thailand and Lao PDR.
More than 600 million people, mainly in Asia, are at risk of
infection with these two liver flukes (Petney et al 2013 Int J
Parasitol 43, 1031-46).
The infections are associated with hepatobiliary diseases including
hepatomegaly, cholangitis, fibrosis of the periportal system,
cholecystitis, gallstones and are major aetiological agents of bile
duct cancer, cholangiocarcinoma (CCA). O. viverrini and C. sinensis
are classified as Group 1 carcinogens metazoan parasites that are
carcinogenic to humans by the the International Agency for
Research on Cancer, World Health Organization (WHO) (Bouvard et
al., 2009 Lancet Oncol 10, 321-2). Therefore, not only do these
liver flukes cause pathogenic helminth infections, they also are
carcinogenic in humans in similar fashion to several other more
well known biological carcinogens, in particular hepatitis viruses,
human papilloma virus and Helicobacter pylori.
The liver fluke endemic area of Khon Kaen, Northeast Thailand has
reported the highest incidence of liver cancer in the world (Shin
et al 2010 Cancer Sci. 101, 579-85; Sripa et al. 2014 Acta Tropica
141, 361-367). In regard to socioeconomic impact, it was estimated
20 years ago that the total direct cost of O. viverrini infection
to the work force (between the age of 15 and 60 years) in Northeast
Thailand was US$ 80 million per annum. More recently, it has been
reported that liver and bile duct cancer, the end-stage consequence
of liver fluke disease, ranks number five in Thai males among all
diseases with highest number of disability-adjusted life years
(DALYs) (see Sripa et al., 2012 Trends Parasitol 28, 395-407).
The liver flukes used here as a source of genomic DNA for the WGS
were obtained by collecting the metacerciae of O. viverrini from
wild caught fresh water fishes in the vicinity of Khon Kaen City,
Khon Kaen province, Thailand. Hamsters were experimentally infected
with these metacercariae at Khon Kaen University, after which adult
O. viverrini worms were recovered from the biliary tract of the
hamsters at euthanasia six weeks after infection. [The outbred,
male Syrian (golden) hamsters (Mesocricetus auratas) were reared at
the animal facilities of the Faculty of Medicine, Khon Kaen
University, Khon Kaen, Thailand. Protocols for the experiments were
approved by the Animal Ethics Committee of Khon Kaen University,
approval number AEKKU25/2554, according to the Ethics of Animal
Experimentation of the National Research Council of Thailand.]
Genomic DNAs were recovered from pools of 10 to 20 adult
(hermaphroditc) flukes and provided by Dr. Banchob Sripa
(banchob@kku.ac.th) and Dr. Paul Brindley
(pbrindley@email.gwu.edu).
This assembly consists of fragments, 3kb and 8kb insert whole
genome shotgun libraries. The sequences were generating on the
Illumina platform. An initial assembly was generated using
Allpaths_LG. To improve scaffolding and contiguity, we used our in
house tool Pygap (Gap closure tool), which uses the Pyramid
assembler with Illumina paired reads to close gaps and extending
contigs. An alternate assembly was generated by first using flash
(fast length adjustment of short reads Bioinformatics 27:21 (2011),
2957-63) to merge the Illumina fragments. These reads were then fed
to the Newbler assembler (Roche). Newbler contigs > 500 bases were
used to fill gaps in the allpaths scaffolds using PBJelly (PLoS ONE
7(11): e47768. doi:10.1371/journal.pone.0047768). The final step
was using L_RNA_scaffolder (BMC Genomics 2013, 14:604), which uses
transcript alignments, to improve contiguity.
The repeat library was generated using Repeatmodeler (A. Smit, R.
Hubley http://www.systemsbiology.org/). The Ribosomal RNA genes
were identified using RNAmmer
((http://www.cbs.dtu.dk/cgi-bin/nph-sw_request?rnammer ) and
transfer RNA's were identified with tRNAscan-SE (Lowe and Eddy,
1997). Non-coding RNAs, such as microRNAs, were identified by
sequence homology search of the Rfam database
(http://selab.janelia.org/software.html). Repeats and predicted
RNA's were then masked using RepeatMasker (A. Smit, R. Hubley & P.
Green http://repeatmasker.org). Protein-coding genes were predicted
using a combination of ab initio programs Snap (I. Korf, 2004),
Fgenesh (Softberry, Corp) and Augustus (M. Stanke, et. Al 2008) and
the annotation pipeline tool Maker (M. Yandell et. al., 2007) which
aligns mRNA, EST and protein information from same species or
cross-species to aid in gene structure determination and
modifications. A consensus gene set from the above prediction
algorithms was generated, using a logical, hierarchical approach
developed at the Genome institute. Gene product naming was
determined by BER (JCVI: http://ber.sourceforge.net).
Our goal is to explore this WGS draft sequence of O. viverrini to
better define proteins and other metabolites involved in parasitism
that impact health and disease and are relevant to host-parasite
relationships, parasitism, carcinogenesis and other biological and
pathological processes.
For information regarding this assembly or project, or any other
GSC genome project, please visit our Genome Groups web page
(http://genome.wustl.edu/genome_group_index.cgi) and email the
designated contact person. For specific questions regarding the O.
viverrini genome project contact Makedonka Mitreva
(mmitreva@genome.wustl.edu) at Washington University School of
Medicine. The National Human Genome Research Institute (NHGRI) of
the National Institutes of Health (NIH) provided funds for this
project.
##Genome-Assembly-Data-START##
Current Finishing Status :: High-Quality Draft
Assembly Method :: allpaths LG v. 43357 (2012-12-28)
Assembly Name :: O_viverrini_1.0.pg.lrna
Genome Coverage :: 23x
Sequencing Technology :: Illumina
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..368823
/organism="Opisthorchis viverrini"
/mol_type="genomic DNA"
/submitter_seqid="O_viverrini-1.0_Cont67"
/isolate="Khon Kaen"
/isolation_source="wild caught freshwater fish"
/db_xref="taxon:6198"
/chromosome="Unknown"
/sex="hermaphrodite"
/dev_stage="adult"
/lab_host="hamster"
/country="Thailand: Khon Kaen province"
/note="pooled from 10-20 individuals"
gene 370..39168
/locus_tag="X801_00515"
mRNA join(370..396,1695..1816,1856..2005,2050..2194,2236..2343,
2363..2459,5611..5745,8424..8520,8751..8779,10792..10911,
11196..11797,13165..13229,14086..14152,14593..14741,
17455..17503,18351..18468,23843..23979,27553..27655,
29964..30084,34511..34616,36251..36361,36536..36649,
39106..39168)
/locus_tag="X801_00515"
/product="hypothetical protein"
CDS join(370..396,1695..1816,1856..2005,2050..2194,2236..2343,
2363..2459,5611..5745,8424..8520,8751..8779,10792..10911,
11196..11797,13165..13229,14086..14152,14593..14741,
17455..17503,18351..18468,23843..23979,27553..27655,
29964..30084,34511..34616,36251..36361,36536..36649,
39106..39168)
/locus_tag="X801_00515"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23559.1"
/translation="MLHEQLPVQELVVRIHVESEDAFKDYPLLVVVKQRNEVTSFRVP
TVVNELSVYHNVSRTLCPLKLSSDFSVNFTVELSTFSLKNVSFKFRAESVTDFDLRKD
DPLNFLLRPAQPVYFRYQFTHPLDSVAVKVVSPDNVCMVLSLQTMQCPVSDGLDTVQN
AGLYQTVTNLGVISANVCSMRTSTELQYPDGFYVVLVLKATDFACTGMERLLPIPTGD
SSYSHPNNFNYAYLSLIVAHFQQHREPNRQNKNVTLKILPAPSRWDYIIPISGAFGFF
ALFYLAGIIIICTSRYLPHRHERSVDLTPQHFDLLSEEAEAFCQPTDVVRNYGTATHI
TTTHPSLHTDSSLVDMLDTEESESRSLKPPKPLCTQRARRGNDSQGGDSRSRTSTTQQ
LPSTSHHADVSLSVPRGENSLGGSTASLNHSRTLIMEHTREHHGWFSSSDEEQPEGHF
VGAQDTLDKYSHRSPQVRSFTPGSVIQHEVSETRPITELRRPNHSPVSTGTTQTSAPD
KQAPSERGSREVLTNAERNTDDVTILLSQNDMFRIVPVSNLSRKRYTTLNRKYLLYFW
YLIIISIFYGLPAIQLIMIYQKTLVETGNEDLCYYNFECARPLGIFTAFNNIISNIGY
IMLGLLFLTATARRDLIHRRRRKLDPEVTETRGLPQHYGLYYAMGLALTMEGIMSACY
HMCPSFSNFQFDTAYMYILAMLIILKIYQTRHPDVNASAHSAYMVMAVVIFLGVTGVV
YGSQTFWIAFTILFLLMSVVLTGEIYYMGQWNIDYCLPRRLFSMIKSDGIRSLRPMYL
ERMILLLVANLVNFALAGYGVATRPRDFSSFLLSVFMINMMVYTLFYIFMKIRHKERI
LLAPILYMVAGLVCWSAAIYFFFLRNTTWEVTPAQSRALNHPCLLFDFYDAHDIWHFL
SASSMFFSFMMLMNLDDDLVDRPRDQIAVF"
assembly_gap 32602..32701
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<48312..59191)
/locus_tag="X801_00516"
mRNA complement(join(<48312..48525,52248..52402,58487..58679,
58911..59191))
/locus_tag="X801_00516"
/product="hypothetical protein"
CDS complement(join(<48312..48525,52248..52402,58487..58679,
58911..59191))
/locus_tag="X801_00516"
/inference="protein motif:HMMPfam:IPR010432"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23560.1"
/db_xref="InterPro:IPR010432"
/translation="MTTEWSNYYASLEQWVKEQHQALSVSEQQMRQAAEEQNRWWRAS
FLAQKQYEKEMQRFQLRASQNLAASQRHSAAQVDNRHIPVQCKPVDFPFITLRRARIW
AKSELRKSELFLTRLEQLLFGGDGEQDMSDADGDLFDLDLLLIVDHIGRFFGAIIEAI
CISRCFFGRGPGGATIGKWIMNLRVVSCDEIIPLPEMVEVVPGSNLGIIRAIVRSVLK
STSFLTIFSFICMMLFRYHRCTYDIIAGSLVVQPLPIVVQMPDQNVGNDMGAAVQPNE
HENQR"
assembly_gap 61687..65254
/estimated_length=3568
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 89052..89405
/estimated_length=354
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 97872..118919
/locus_tag="X801_00517"
mRNA join(97872..98028,104352..104440,104536..104637,
105449..105644,105812..106125,110474..110581,
114952..115031,118805..118919)
/locus_tag="X801_00517"
/product="F-box domain protein"
CDS join(97872..98028,104352..104440,104536..104637,
105449..105644,105812..106125,110474..110581,
114952..115031,118805..118919)
/locus_tag="X801_00517"
/codon_start=1
/product="F-box domain protein"
/protein_id="OON23561.1"
/translation="MSGGSASGLTTEDSDTTDDEDSESSTVATCTTEQTARKSYQDLA
DKVEKKLLTIRNPDHWATGDGFDDLPAEALDPEDEPYKKTHISALPHELLLRIFRWAV
GSHLDTRILGRLARVCRGFYLLACDSSIWRSICLRLWPRLLDHHSHGVRGEQLTAAVP
LHYGYKDWRDMAIHRPHVLLDGCYLCRITYVRPGEALSGIYRPMHLVVYYRGIRFYPD
GHVNMLTSYHAPNAVVAALGKPFAITQRDASALPTGTEADSSGLVQDSLSASGGGLLQ
GTYVLVDPDLIVCTLFRPRNKEKLHPFRRRRQAQLFSSEQQVTYNIRFKLSSSKKRLH
NVLHWDSYTIRQLNTTTQTDQLITLNVFSDQFPECRFSPVRSYVSTVAPEPL"
gene 127508..135426
/locus_tag="X801_00518"
mRNA join(127508..127708,135214..135426)
/locus_tag="X801_00518"
/product="glutaredoxin-like protein"
CDS join(127508..127708,135214..135426)
/locus_tag="X801_00518"
/inference="protein motif:HMMPfam:IPR008554"
/note="KEGG: cqu:CpipJ_CPIJ019232 3.2e-08 acetyl-CoA
acetyltransferase, mitochondrial; K00626 acetyl-CoA
C-acetyltransferase"
/codon_start=1
/product="glutaredoxin-like protein"
/protein_id="OON23562.1"
/db_xref="InterPro:IPR008554"
/translation="MLSSRLSSLVTRPPRIFQRAPPLVTINRLTSQTLAYPSVPTLVM
FTKPDCSLCRAAIEQLRPYANKYFRLQLVDITEAKNSVWRKYQYDIPVFHILSVGTQL
THENKGTYLMKHHVDWNRLKNSIPELDQCPELDEY"
assembly_gap 133310..133633
/estimated_length=324
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<138738..147955)
/locus_tag="X801_00519"
mRNA complement(join(<138738..138959,139070..139252,
142165..142477,144024..144164,147819..147955))
/locus_tag="X801_00519"
/product="hypothetical protein"
CDS complement(join(<138738..138959,139070..139252,
142165..142477,144024..144164,147819..147955))
/locus_tag="X801_00519"
/note="KEGG: ddi:DDB_G0274493 8.7e-08 lig1; DNA ligase I;
K10747 DNA ligase 1"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23563.1"
/translation="MVPRMYILTDMRLSDHHAIVPSLDQAKVEQEIKLDHSGGIHSIP
LRKMTLRILLKRFMFFQKTNSAAHPGFAQSLKRHITVAKPSLSTVNTKLVRQRSCPRT
RKDQGAQEQRVDDAICHREDESNTPSYSLDFTDDLCSHSRPDEIEQDPNLLDSSSKNS
DRSAIATKVEELTPWERWLLQKEKIRRQELKRLRQKEANLRAKQAQAAASQAEKEQRS
LAKCKEWLESKQRLLKERNEAAAMARKEQLLKERFKKESKKLESERKYALWCEQKKHE
AKETAELIRQKELAAAASAQRRKEEAMVKFVAWMKSAQQRKPNPPVSYGYADGTVI"
gene 162517..164347
/locus_tag="X801_00520"
mRNA join(162517..162771,162804..162988,163028..163162,
164245..164347)
/locus_tag="X801_00520"
/product="ubiquitin--protein ligase"
CDS join(162517..162771,162804..162988,163028..163162,
164245..164347)
/locus_tag="X801_00520"
/inference="protein motif:HMMPfam:IPR000608"
/note="KEGG: smm:Smp_055960 8.0e-72 ubiquitin-conjugating
enzyme E2r; K02207 ubiquitin-conjugating enzyme E2 R"
/codon_start=1
/product="ubiquitin--protein ligase"
/protein_id="OON23564.1"
/db_xref="InterPro:IPR000608"
/translation="MSEFTDQSRTGLQLGLPRTQTCDLFMASKKPQSSAVKALQKELL
ELNATPVEGFKVNISSVENMFVWDVAIFGPPKTLYEGGYFKARLFFPPDYPYSPPSMQ
FLTKMFHPNIYADGEVCISILHSPGEDPQSDELASERWNPTQNVRTILLSVISLLNEP
NIHSAAHVDASISYRKWKESNGKENEFEQIVSKQSNNRRITVPTVARAISLNFGAEPN
GATTPIG"
gene 177465..178342
/locus_tag="X801_00521"
mRNA join(177465..177541,177925..178342)
/locus_tag="X801_00521"
/product="hypothetical protein"
CDS join(177465..177541,177925..178342)
/locus_tag="X801_00521"
/note="KEGG: smm:Smp_055960 7.8e-13 ubiquitin-conjugating
enzyme E2r; K02207 ubiquitin-conjugating enzyme E2 R"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23565.1"
/translation="MHHSPCRNTWATLVTETAQQTHGLFYLVLQTHADAEKDGVHVPT
TVEEYCVSGRPGNDPDQSETGQDGSDFDDYYEYYPSDVDDADEDDDVACSESLDAGAD
SAMEENEGEVASRPKHNAQPNQSAANVSPGQRVGDGDRESSSSQTSRAASSVSKATHD
SENS"
assembly_gap 208140..208266
/estimated_length=127
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 212774..>229928
/locus_tag="X801_00522"
mRNA join(212774..213081,217914..218190,220075..220495,
229624..>229928)
/locus_tag="X801_00522"
/product="hypothetical protein"
CDS join(212774..213081,217914..218190,220075..220495,
229624..>229928)
/locus_tag="X801_00522"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23566.1"
/translation="MMSVNTFTGVFQLFTEKLNYSHHRDGSQDETSVRYTKELRGFMK
GEKSFSKLIVRSDRHLWTQALQLLNTGKTPITRSIREGTEETQPMILVDNECWICLKL
KLTKRWTKLLNLQCLEKFGRSKSFSFPFIVNVKRNSKEYDTLIQKITGRSSRNQASKR
GRTRVCSSLGHRGREHAVGKTQESGFLAPCSKDDEVKSTNIVLIPLYPAASDQLHNSV
SYSSEHHDNKSCAQVDLDLDSVERTSDECIGNNKPTALLCMSLRNASTNSSCALSAGD
DLGRWYITFVPKVHQTKIVEFFFIACLTGHTPQALLGQTLDSFIHPSDSRRLSQWLSA
DASNGKNEMEKEADTLECRWRCANCTYTWLSISRVCSTQVLDQRTTTQPIQNNLHFYR
LTWLSGPTDLENFFDPISVSPVAVRSDSLSLSSEAMDKWCREMRF"
assembly_gap 220989..221088
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 234763..>240625
/locus_tag="X801_00523"
mRNA join(234763..234819,237047..237561,238837..239486,
240369..>240625)
/locus_tag="X801_00523"
/product="hypothetical protein"
CDS join(234763..234819,237047..237561,238837..239486,
240369..>240625)
/locus_tag="X801_00523"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23567.1"
/translation="MNEQLVGASQTYINQQFVQPRSEEPPVKVTRRSHEPPHQAKKAE
LTDIPPIQLGPESFNNFSSVPPKQNTGFWNPTTQKAYLEKEYPGQMPTAPQFTQYLHA
RRLQEECNNRKLYIPQHLMVPNVNGKRSFCQAFAERVPCAVKGTPCCVKPHAILPNPL
ACMCGVQKCRAFVQQYCCSGTSHSQTTPGGPCATCSLDQCASHQRASIICELHPHVNQ
IWIMKNNWHSSNTCPHNESTNTTGTQPAANLLSGSNGSGSNGVTPGSCYHHACLYHSN
RCRVENTNPFPTCKCVSSVSSIPCCCSGNPTTPLMNTFNYAISNWCNNQQMLPQYPLN
HLNENGISQRGGEGAPNVFFSAAIKPATIPMVSNPIPNFAADGGRVCTGIQAQSNYQG
PLAGDFRNCGEPMENVVLPLEVYETKFLAHPHSSLPPEIITNTPAKPTTQPKQPYPST
TEGLLKQTHRFAKFEHLQPVSSKVLRPPSSHNESAEGQTNDLT"
assembly_gap 253777..253876
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<264443..>265107)
/locus_tag="X801_00524"
mRNA complement(join(<264443..264561,264673..264798,
264834..264889,264925..265034,265069..265107))
/locus_tag="X801_00524"
/product="leucine Rich repeat-containing domain protein"
CDS complement(join(<264443..264561,264673..264798,
264834..264889,264925..265034,265069..>265107))
/locus_tag="X801_00524"
/note="KEGG: dme:Dmel_CG10839 8.6e-36 CG10839 gene product
from transcript CG10839-RA K10411"
/codon_start=1
/product="leucine Rich repeat-containing domain protein"
/protein_id="OON23568.1"
/translation="DKPTTIKEAIKKWEEKTGQKASEAKEVKLYAQYPPIEKLDASLS
TLTACEKLSLSTNCIEKITNLNGLKNLKILSLARNNIKNLNGLEVLGDTLEQLWISYN
ILERLKGIGECRNFRNQVIILGNPLEENNSETWRDEACRRLPRLKKLD"
assembly_gap 267859..267958
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 271979..272123
/estimated_length=145
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 274750..274849
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 278043..>298298
/locus_tag="X801_00525"
mRNA join(278043..278078,278114..278289,285149..285311,
292578..292796,298131..>298298)
/locus_tag="X801_00525"
/product="Pyrimidine 5'-nucleotidase"
CDS join(278043..278078,278114..278289,285149..285311,
292578..292796,298131..>298298)
/locus_tag="X801_00525"
/note="KEGG: smm:Smp_097180 2.9e-74 hypothetical protein;
K01081 5'-nucleotidase"
/codon_start=1
/product="Pyrimidine 5'-nucleotidase"
/protein_id="OON23569.1"
/translation="MDPELTESTKSRLTYLRDKYYPVEIDPTLSDAEKIPQMVEWWRL
SHESIVSCGLHRDALAKTVRECGLVLRDGVQEFTELLRFHQIPLLIFSAGLGDVIELL
LESFSMYTENVRVVSNFMQFSDEGLLVGFTDPIIHSFNKTAASIANGDYARLSSQRPC
VLLLGDSTGDVHMADGATVDDPTGISGTVLRIGFLNEAVEANLEKYKTLYDIVLVNDD
TFAVPLAVMKSILQCHQAVADGIAAPTTNNTDDIEL"
assembly_gap 294164..294978
/estimated_length=815
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene <308503..>311947
/locus_tag="X801_00526"
mRNA join(308503..308763,309472..309554,310839..311075,
311800..>311947)
/locus_tag="X801_00526"
/product="hypothetical protein"
CDS join(<308503..308763,309472..309554,310839..311075,
311800..>311947)
/locus_tag="X801_00526"
/codon_start=1
/product="hypothetical protein"
/protein_id="OON23570.1"
/translation="STYKTDVRPLCFLEFCELVCKILLWAVFPSLYPLVTCLECFVGN
FSIPETTLRGQRLSNNEFLVPPNVRFASFVPFERCCETEFETVFIKECRHTGLIPYML
GGHQPIVCRGEALLETRCSDPGEQIQTQPCVIRRIHKNTMFCSSNIHLYCCVFNSILT
IVVVVVLIHIMTKIYRPTTAKNPSGPVSVPHCESGRRPNDLGANPHLCRFNSCYWCSL
QFPELKEHMKEFYSDTFCYTSGRRI"
gene complement(321713..353100)
/locus_tag="X801_00527"
mRNA complement(join(321713..321868,323033..323182,
323878..324186,326761..326955,333306..333622,
342971..343169,352888..353100))
/locus_tag="X801_00527"
/product="eukaryotic aspartyl protease"
CDS complement(join(321713..321868,323033..323182,
323878..324186,326761..326955,333306..333622,
342971..343169,352888..353100))
/locus_tag="X801_00527"
/inference="protein motif:HMMPfam:IPR001461"
/note="KEGG: spu:585631 4.6e-53 hypothetical LOC585631;
K04521 beta-site APP-cleaving enzyme 1 (memapsin 2)"
/codon_start=1
/product="eukaryotic aspartyl protease"
/protein_id="OON23571.1"
/db_xref="InterPro:IPR001461"
/translation="MQLSLALIIFIFATTHCKTNIILPIRLRRTENEALFAASFPNSA
KMAVSNLAGLPGMGYYLPVTLGNPPQELNLLVDTGSADLAVAGRKLANVDRWFNASAS
STLECSGFSKQVRYQQGSWIGPLCKDRLTLVLRNMSHNQSITRLPPVPIHFSLIEDAQ
NFFLSHYGSTWEGIIGLGYPKLMMDNIPSRQNQVEQVLSHSPWIRVLWKLFEQTPEQL
HPFETLTSAWNLPNMFSLLLCGLTQQSFSVIGSSPVEMNGLLLIGGMNLTGMTQTVNT
ILYTPVREPWFYEVVLTDLRVEEQSVVEDCKELNVEFSIVDSGTTNIQVPERVFQPLL
GHIKTYVAKQSSSTATMLQNYASFWAGASMLCEASSDNQIGGASGLPHTLFPVIEFQM
LALGADMNKEALSLTLSPQQYIRFVGRHPKDGVMRDCFAFGIRPHHSGTILGAVFLEG
FFTVFHRNSLKVGAAKLFNLHQPHFNTKMDAFETNLFTCTFNCVHTIEKGFDASSKGR
VCDQ"
assembly_gap 353430..353541
/estimated_length=112
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
CONTIG join(LASN01000956.1:1..32601,gap(100),LASN01000957.1:1..28985,
gap(3568),LASN01000958.1:1..23797,gap(354),LASN01000959.1:1..43904,
gap(324),LASN01000960.1:1..74506,gap(127),LASN01000961.1:1..12722,
gap(100),LASN01000962.1:1..32688,gap(100),LASN01000963.1:1..13982,
gap(100),LASN01000964.1:1..4020,gap(145),LASN01000965.1:1..2626,
gap(100),LASN01000966.1:1..19314,gap(815),LASN01000967.1:1..58451,
gap(112),LASN01000968.1:1..15282)
//