LOCUS KN716607 97522 bp DNA linear CON 12-MAR-2015
DEFINITION Dictyocaulus viviparus strain HannoverDv2000 unplaced genomic
scaffold D_viviparus-1.0_Cont458, whole genome shotgun sequence.
ACCESSION KN716607 AZAF01000000
VERSION KN716607.1
DBLINK BioProject: PRJNA72587
BioSample: SAMN02873952
KEYWORDS WGS; HIGH_QUALITY_DRAFT.
SOURCE Dictyocaulus viviparus (bovine lungworm)
ORGANISM Dictyocaulus viviparus
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
Rhabditina; Rhabditomorpha; Strongyloidea; Metastrongylidae;
Dictyocaulus.
REFERENCE 1 (bases 1 to 97522)
AUTHORS Mitreva,M.
TITLE Draft genome of the bovine lungworm Dictyocaulus viviparus
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 97522)
AUTHORS Mitreva,M., Pepin,K.H., Abubucker,S., Martin,J., Minx,P.,
Warren,C., Palsikar,V.B., Zhang,X. and Wilson,R.K.
TITLE Direct Submission
JOURNAL Submitted (12-NOV-2013) The Genome Institute, Washington University
School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA
COMMENT Dictyocaulus viviparus, the bovine lungworm, is the cause of
parasitic bronchitis in cattle (husk, verminous pneumonia,
dictyocaulosis) with a world wide distribution in temperate areas.
The predominant hosts are cattle, but it can also infect deers.
Infection occurs on pasture as infective larvae develop from L1
that are shed with the feces to free-living infective L3 that are
ingested by cattle while grazing. Free-living larvae do not feed;
survival on pasture is therefore limited to a few months depending
on temperature and humidity. The parasite is able to interrupt
development inside the host. Infective larvae that have been
exposed to low temperatures on pasture before infection
subsequently develop only to preadult larval stages that survive
winter conditions as hypobiotic larvae in the lung and resume
development in spring. These animals contaminate pastures the
following spring and represent the major source of infection for
other susceptible cattle. The strain being sequenced
(HannoverDv2000) was obtained from the laboratory of Drs. Thomas
Schnieder and Christina Strube (Christina.Strube@tiho-hannover.de)
and has been maintained in calves since August 2000. Worm isolation
and DNA extraction was performed by Christina Strube and/or the
Genome Institute's production team.
This assembly consists of fragments, 3kb and 8kb insert whole
genome shotgun libraries. The sequences were generated on the
Roch/454 platform and assembled using Newbler. To improve
scaffolding, inhouse tools CIGA (Cdna tool for Improving Genome
Assembly) and Pygap (Gap closure tool) were used to map 454 cDNA
reads using blat to the genomic assembly to link genomic contigs
based on cDNA evidence. Only joins confirmed by additional
independent data typing were accepted and close gaps followed by
the Pyramid assembler and Illumina paired reads to closing gaps and
extending contigs
The repeat library was generated using Repeatmodeler (A.F.A. Smit,
R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA
genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic
Acids Res.) and transfer RNA's were identified with tRNAscan-SE
(Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as
microRNAs, were identified by sequence homology search of the Rfam
database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.).
Repeats and predicted RNA's were then masked using RepeatMasker (A.
Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding
genes were predicted using a combination of ab initio programs Snap
(Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V.
2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008
Bioinformatics) and the annotation pipeline tool Maker (M. Yandell
et. al., 2007 Genomc Research) which aligns mRNA, EST and protein
information from same species or cross-species to aid in gene
structure determination and modifications. A consensus gene set
from the above prediction algorithms was generated, using a
logical, hierarchical approach developed at the Genome institute.
Gene product naming was determined by BER
(http://ber.sourceforge.net).
Our goal is to explore this WGS draft sequence of D. viviparus to
better define proteins involved in nematode parasitism that impact
health and disease and are relevant to both host-parasite
relationships and basic biological processes.
For information regarding this assembly or project, or any other
GSC genome project, please visit our Genome Groups web page
(http://genome.wustl.edu/genome_group_index.cgi) and email the
designated contact person. For specific questions regarding the D.
viviparus genome project contact Makedonka Mitreva
(mmitreva@genome.wustl.edu) at Washington University School of
Medicine. The National Human Genome Research Institute (NHGRI) of
the National Institutes of Health (NIH) provided funds for this
project.
##Genome-Assembly-Data-START##
Current Finishing Status :: High-Quality Draft
Assembly Method :: Newbler v. 2.6
Assembly Name :: D_viviparus_9.2.1.ec.pg
Genome Coverage :: 12.20x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..97522
/organism="Dictyocaulus viviparus"
/mol_type="genomic DNA"
/submitter_seqid="D_viviparus-1.0_Cont458"
/strain="HannoverDv2000"
/isolation_source="Cow lung"
/host="cattle"
/db_xref="taxon:29172"
/chromosome="Unknown"
/country="Germany"
/lat_lon="51.00 N 9.00 E"
/collection_date="Aug-2000"
/collected_by="Drs. Thomas Schnieder and Christina Strube"
assembly_gap 1270..1369
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 4061..8814
/locus_tag="DICVIV_11046"
mRNA join(4061..4139,5088..5221,5281..5389,6065..6216,
6361..6444,6643..6788,7156..7222,7308..7406,7786..7959,
8776..8814)
/locus_tag="DICVIV_11046"
/product="GDSL-like protein"
CDS join(4061..4139,5088..5221,5281..5389,6065..6216,
6361..6444,6643..6788,7156..7222,7308..7406,7786..7959,
8776..8814)
/locus_tag="DICVIV_11046"
/inference="protein motif:HMMPfam:IPR001087"
/note="KEGG: hsa:151056 6.1e-46 PLB1, PLB, PLB/LIP, hPLB;
phospholipase B1"
/codon_start=1
/product="GDSL-like protein"
/protein_id="KJH42944.1"
/db_xref="InterPro:IPR001087"
/translation="MLKPSATIPDDVNKVRPADIKAIAAIAAKTLGGKLHCDPEMLKP
SATIPDDVNKVRPADIKAIAAIGDSFIVAARSTNFLDDPPDIFPGNSFISGGDETVEE
HITVAKILRIFNPTVMGVSHGAGYGNMGFNVATGGATCKDVIVQAKELIRRMKEGYVN
LNEDWKLIWIFIGTNDIGRVGCISDEESTSRDVYKSKLEEGISYLLYNLNRTIISIIP
IWNPHLTVDARWLIDKGKRMECGHYSTRNRDVLAEEYRKVAYELQNEKRFDHKNFTVV
VQGFMDEIRDAFRKKSGEYDKSFYGSDSFHLSKYGNAVMAKFLWNNLLEPVGNKTTNA
DLGDDSVPIKCPTMERPYIQTLGNRM"
assembly_gap 4783..4882
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 10494..12665
/locus_tag="DICVIV_11047"
mRNA join(10494..10633,11498..11641,12506..12665)
/locus_tag="DICVIV_11047"
/product="hypothetical protein"
CDS join(10494..10633,11498..11641,12506..12665)
/locus_tag="DICVIV_11047"
/inference="protein motif:HMMPfam:IPR021134"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42945.1"
/db_xref="InterPro:IPR021134"
/translation="MTVNYMREAATARLGTFLKLLFRWRGSVWKTIWKELAIYLFFYF
NLSVFYRFMLKGNSYGVIFELFVTHCRNLSRGAATVLSFALGFYVSQISDRDISERIR
RRFPTYNHLVRMPFFHYSSRNYNLNSFIGGKILPSSSSISELLPL"
gene 13407..19503
/locus_tag="DICVIV_11048"
mRNA join(13407..13550,13773..13850,13913..13981,14170..14259,
14328..14399,15272..15355,15603..15725,16251..16337,
16440..16496,16965..17108,17732..17806,17942..17986,
18542..18629,18730..18753,19187..19503)
/locus_tag="DICVIV_11048"
/product="hypothetical protein"
CDS join(13407..13550,13773..13850,13913..13981,14170..14259,
14328..14399,15272..15355,15603..15725,16251..16337,
16440..16496,16965..17108,17732..17806,17942..17986,
18542..18629,18730..18753,19187..19503)
/locus_tag="DICVIV_11048"
/inference="protein motif:HMMPfam:IPR021134"
/note="KEGG: tet:TTHERM_00467740 0.0013 Protein kinase
domain containing protein; K08857 NIMA (never in mitosis
gene a)-related kinase"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42946.1"
/db_xref="InterPro:IPR021134"
/translation="MTTSEKARIETEDIKRVYWMPIEWANQLLKKCYLKDQIDPYHYG
ILCETILKYREMLHNLLSFDWINVPLVYTQVVHVCTVAYFGIICFASQSINEPESEMW
INEFVIPVFAIYEFIFFVGWLKVAQVMLNPFGMDDDDFEIDMVIERNLQIGYSFVDSM
YDKLPPLIYVDVAALPHTKASMALITKANPMVGSVANVDVPRAEQQVISKADVEIIRK
LVGPRFGRRFHYDHSKYNNDMDRITNSPEEHDLGKKSSKSNSFLKKHIIQPNNTPNPS
REHDTSVAKKVGSSYGIHGGDVIGEVQKKLERHKNALHTENQHDSLEKTQSPPSRDCK
TTSAQSKILNPTQFSSELIEHKNSSKPFKLGTIRHSSLQHTQKTAPNTLRESSTTALT
GGWGKSEEKILEKLSTTKFNSKDKISRNGRQLHGSTENRRITEEKKEILTLENPTEHS
TPKLRTKLTARKKESRKKMTPKPESLLSSATPTSIKTWKSSHHLKKSK"
gene 21073..22704
/locus_tag="DICVIV_11049"
mRNA join(21073..21144,21284..21439,21627..21728,21781..21881,
21962..22055,22380..22489,22596..22704)
/locus_tag="DICVIV_11049"
/product="hypothetical protein"
CDS join(21073..21144,21284..21439,21627..21728,21781..21881,
21962..22055,22380..22489,22596..22704)
/locus_tag="DICVIV_11049"
/inference="protein motif:HMMPfam:IPR000504"
/inference="protein motif:HMMPfam:IPR000571"
/note="KEGG: smm:Smp_123750 3.7e-16 arylformamidase"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42947.1"
/db_xref="InterPro:IPR000504"
/db_xref="InterPro:IPR000571"
/translation="MANMNDRMSGAEYLASIYGTEKDKVNCSFFFKIGACRHGDKCSR
THIIPTFSQTVLLKNLYHNPVIDMRQADACTKVGMMNKDEQQYFDEFFEEIFTEVNEK
YGEIEEMEVCDNIGEHMIGNVYVKFAREEDAERCVKALDNRWFNGQAIYAELSPVTDF
REACCRQYELGGCNKGAFCNFMHLKPISSDLRRKLYKGRRRSPPRYHGGKERDREMRS
GRGRGDFGGGRDFGGGGGRRRSRSPRRKF"
gene 25542..27957
/locus_tag="DICVIV_11050"
mRNA join(25542..25634,25687..25809,26459..26541,26616..26705,
26872..27034,27469..27675,27859..27957)
/locus_tag="DICVIV_11050"
/product="HAD hydrolase, family IE"
CDS join(25542..25634,25687..25809,26459..26541,26616..26705,
26872..27034,27469..27675,27859..27957)
/locus_tag="DICVIV_11050"
/note="KEGG: rcu:RCOM_0945550 8.7e-27 Cytosolic
5'-nucleotidase III, putative"
/codon_start=1
/product="HAD hydrolase, family IE"
/protein_id="KJH42948.1"
/translation="MEKILKSLYFSSSSTGSILSDFRVGGKEKIMVVTDFDYTLTLRQ
DDSGNKGCLTHEVFQTLKQKNGCTVEALQHELNKHYETALSNCSTKDDRISVQIDWWN
ESHDIIVRSGVHCDQLPTIVRNSKIIWRKNVLHLIKLLEEMSVPLLIYSAGITDIIKE
AIRQLIGYFPCNISIVANRMHFDEHGRLSGFTMPPLCSLTKTMVRLRGLAKEVDEIYK
DRTNVILIGDSDEDAIMLNGWEERVFLKIRLVQDKISQPHSFDLVILGSDCHQLIDIV
KYIIFPQQT"
gene complement(28758..29579)
/locus_tag="DICVIV_11051"
mRNA complement(join(28758..28817,29108..29224,29523..29579))
/locus_tag="DICVIV_11051"
/product="hypothetical protein"
CDS complement(join(28758..28817,29108..29224,29523..29579))
/locus_tag="DICVIV_11051"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42949.1"
/translation="MPCEKKSGEKRESHCLDSQIHQTFRRPLKEKYSRYAMLTIKKQR
CRNTKKINDCSKSESNDKSGKKHNLNKGTHDEP"
assembly_gap 31850..31949
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 32964..33063
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 38692..43133
/locus_tag="DICVIV_11052"
mRNA join(38692..38759,39253..39373,39874..39996,40214..40299,
40544..40633,40910..41075,42133..42233,42757..42859,
43014..43133)
/locus_tag="DICVIV_11052"
/product="HAD hydrolase, family IE"
CDS join(38692..38759,39253..39373,39874..39996,40214..40299,
40544..40633,40910..41075,42133..42233,42757..42859,
43014..43133)
/locus_tag="DICVIV_11052"
/inference="protein motif:HMMTigr:IPR018012"
/note="KEGG: oaa:100074606 5.9e-53 hypothetical protein
LOC100074606; K01081 5'-nucleotidase"
/codon_start=1
/product="HAD hydrolase, family IE"
/protein_id="KJH42950.1"
/db_xref="InterPro:IPR018012"
/translation="MPALEVSSVVAFPTHLMIDENNRRLTGHDFDSLLNHPQVMMRDR
NSVEKKLRAMMDDGKENLMVVSDFDYTLSKFEDTQGRRCWTTHGVFDNCVKQIDEDLA
VKFQLLKDKYFPIEFDPNLTIEEKVPFMEEWWNISHQYIVSAKFHRATVEGFVRNAHI
MLRDQTDQMIIQLDRLDVPLVVFSAGIGNIIEMYLKQELGEIPKNIHLISNMMNFDEQ
DIVVSFSEPLIHTFCKNSSVIRKEAEFFHIFRERRNVVLLGDSMGDIHMDVGVEKKAL
TLKIGFLNSNVNSLLDHYMEVYDVVLVQDQSMQIPHKILQAIANGHVKN"
gene complement(43914..46848)
/locus_tag="DICVIV_11053"
mRNA complement(join(43914..44096,45143..45271,45515..45673,
45803..45934,46030..46176,46596..46650,46766..46848))
/locus_tag="DICVIV_11053"
/product="hypothetical protein"
CDS complement(join(43914..44096,45143..45271,45515..45673,
45803..45934,46030..46176,46596..46650,46766..46848))
/locus_tag="DICVIV_11053"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42951.1"
/translation="MQLFQINMSLMKKCCNCRSDLPESNGAGSSSVQCEKCQKNEAKY
GKPTTCKFCQLPAAFHEEKCVYCSHSERKFGAPIPCANCKLRAAFTKDPKKLKPTLCR
MCIIQARANKLTNLAGVTVSSHDHYSGHKRKREEKKKADKQAEPAKVPKRDPFSDSGE
NVMLVQQLRDQISRLNSVISKKDAAMLEKDKKIATLQADLMTAERKHREKVDQLLKEK
DEAIQMVIEKHRQASKQKGKIPYKRKQTPEPLSNVAIRCINNPEIPLHIRSGTMAWPR
NQVIKLPISMAVNGFMICT"
assembly_gap 49211..49310
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 54725..55132
/estimated_length=408
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 57806..64696
/locus_tag="DICVIV_11054"
mRNA join(57806..57832,58194..58276,58835..58925,60514..60633,
61815..61888,63210..63342,63643..63770,63971..64058,
64589..64696)
/locus_tag="DICVIV_11054"
/product="phosphoprotein phosphatase 1 domain protein"
CDS join(57806..57832,58194..58276,58835..58925,60514..60633,
61815..61888,63210..63342,63643..63770,63971..64058,
64589..64696)
/locus_tag="DICVIV_11054"
/inference="protein motif:HMMPfam:IPR004843"
/note="KEGG: cel:R03D7.8 1.5e-31 hypothetical protein;
K01090 protein phosphatase"
/codon_start=1
/product="phosphoprotein phosphatase 1 domain protein"
/protein_id="KJH42952.1"
/db_xref="InterPro:IPR004843"
/translation="MSAQRVGFEVSEKKLVTGQLSFPLLCRVLTNMELRIRKEVIELN
SHEMPTYNKVEFIAGGNGHSLVKVDQMFLSGYENYKITHLLQHGPYVYDWRPFELVSL
LSQATEIFEGESTILILRAPIVIIGDIRGQYQDLHRWLCIVDFPPRSKILFLGGVIDP
EEPGSLDCLAFIAAMKVRFPHDVFLIRGMGETLPIVFHARFRGLNNSAVQSLVLSPKK
KAATRLCNSLPIAARISNRILAVHSGLSHEGKGTYNLLSLDRFFPTGDSTSKFFVHTI
VVYNSLP"
assembly_gap 62417..62516
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 64861..64960
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 66167..66266
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 66417..66577
/locus_tag="DICVIV_14730"
ncRNA 66417..66577
/ncRNA_class="other"
/locus_tag="DICVIV_14730"
/product="U1 spliceosomal RNA"
/inference="nucleotide motif:Rfam:RF00003"
/note="Rfam score 98.56"
gene 67112..69010
/locus_tag="DICVIV_11056"
mRNA join(67112..67184,67813..67871,67936..68046,68870..69010)
/locus_tag="DICVIV_11056"
/product="hypothetical protein"
CDS join(67112..67184,67813..67871,67936..68046,68870..69010)
/locus_tag="DICVIV_11056"
/note="KEGG: cel:R03D7.8 2.1e-09 hypothetical protein;
K01090 protein phosphatase"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42953.1"
/translation="MPPLARNLIFSQPTMKIRMYRDNTDTSQAVYGLGAVNRICREMG
VKLVVRAHNPLQNGICWIGTRHKLISLWSAPAKNSNKVYPNPRAFQGAVINISSDFEI
QVYTLQKQKQVEKSDETLRGENLLL"
gene 72044..76865
/locus_tag="DICVIV_11057"
mRNA join(72044..72109,72357..72453,72539..72595,72656..72782,
73049..73124,73190..73300,73369..73465,73524..73645,
73702..73788,74161..74397,75186..75275,75424..75522,
75586..75714,75771..75996,76142..76236,76294..76411,
76464..76544,76604..76654,76714..76865)
/locus_tag="DICVIV_11057"
/product="hypothetical protein"
CDS join(72044..72109,72357..72453,72539..72595,72656..72782,
73049..73124,73190..73300,73369..73465,73524..73645,
73702..73788,74161..74397,75186..75275,75424..75522,
75586..75714,75771..75996,76142..76236,76294..76411,
76464..76544,76604..76654,76714..76865)
/locus_tag="DICVIV_11057"
/note="KEGG: edi:EDI_048910 1.4e-09 intracellular protein
transport protein USO1"
/codon_start=1
/product="hypothetical protein"
/protein_id="KJH42954.1"
/translation="MELPFVYIDPHEENEDIANVAAFLGQARRIIVNNAVSEEMKRAI
FDLASEYIVDVRKYYQAKEKEALLVKRDADIDEKLHQVERELAEKERINNALRASIEE
KKRNIKLSEITIKKSRNKTSDLFRQRKELEEDCKKLDAKVKESNSTLASSLQTAHKQR
DRLQMLMEKSPRLAKQITERDAVNALEVRLEQLKSEQSDLKAEEERLRKELELQTATP
LRVFIVNFAKITLRTLEMQEKLGEARKVYEQLKAEETERLKSGVCDHEFDASFLSTDY
SFKKVNDHGNILRTELMRPAVVSKPVTPVDDGFSTPETLTSGSSSKSSVEPPQTTEED
IIKARTNAVKSYVTQQSETVARNVESSKNGYKDTIYDENERESIYCLENADKSLGVMR
TADKETVPSTLENNNIVEIADDDMDVNVSQGVIPEDFSDEDGEEQEVEDDGTIAVENE
NDDVAINTNYILINWGVNSAERILSQESEDSTAVEGISRMKIDPINTLKIAQMSETSQ
SILGSDNREKSPISQKFHENSAGRRASMSHRQGCSSQTSENVFNGGSPSPCVDASQEA
GAFFSNLLDQPPSPIQDGIPFNFGSGGITEEIDPSVVLNISTHSNDPGGDFLLLMENE
KRERDRRSEGSSSAAEFSFSMFGTAADPATSGLGGEFAFDFDAPSQCNERIDSTTFQF
NFSEGSNDQEQNNNDGGFNFFDF"
assembly_gap 78773..78872
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 79865..79969
/estimated_length=105
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(80600..86514)
/locus_tag="DICVIV_11058"
mRNA complement(join(80600..80677,80745..81404,81580..81716,
81833..81879,83866..84254,84702..84927,86498..86514))
/locus_tag="DICVIV_11058"
/product="nematode cuticle collagen domain protein"
CDS complement(join(80600..80677,80745..81404,81580..81716,
81833..81879,83866..84254,84702..84927,86498..86514))
/locus_tag="DICVIV_11058"
/inference="protein motif:HMMPfam:IPR002486"
/inference="protein motif:HMMPfam:IPR008160"
/note="KEGG: isc:IscW_ISCW014473 3.6e-37 basement membrane
collagen, putative"
/codon_start=1
/product="nematode cuticle collagen domain protein"
/protein_id="KJH42955.1"
/db_xref="InterPro:IPR002486"
/db_xref="InterPro:IPR008160"
/translation="MCVYDRSAIMKERTTSSLVDLYEMFFVGSHLDSISIKIAYLFYL
HVGIVIAVLIFCGSMVILWQESDKWKKYTDYICHHMSRQRRRARRRLRKSERSTRSGI
SDRSRRSHSHKSSRSHKSSRSEQSTRSAHSNRSHKSSHSHKSSRSHKSSRSEQSTRSA
HSNRSHKSSHSHKSNRSQPSTASHRSNRSHRSSRSNRSGRSNRSSLSERVQEERNDEG
KKKNEPSLFMSKVIVIGSGVTLAFIVCSLVTMVSLLKDIADLQFEVEVGMDEFKEIAE
DTWSHILTKHMNTAGTTDAMQNFAKIVGRRPRAVGFPEQCNCGAKSTGCPPGPPGPPG
PKGIPGEPGVDGEDGRPGAPGIAVAITHDIPGGCITCPAGPPGPRGPPGEQGPPGPPG
IFGQRGPPGLPGPMGEIGPQGDEGQPGIPGRPGPPGPPGEPGTQYTQGMPGHPGPPGQ
RGPPGEPGQQGTPGSDGKPGPQGRPGHPGKDGNPGRNGQPGQRGENGKVGADAEYCPC
PPRGRKRRF"
assembly_gap 90927..91063
/estimated_length=137
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(93847..95077)
/locus_tag="DICVIV_11059"
mRNA complement(join(93847..93921,93994..94407,94512..94754,
94946..95077))
/locus_tag="DICVIV_11059"
/product="nematode cuticle collagen domain protein"
CDS complement(join(93847..93921,93994..94407,94512..94754,
94946..95077))
/locus_tag="DICVIV_11059"
/inference="protein motif:HMMPfam:IPR002486"
/inference="protein motif:HMMPfam:IPR008160"
/note="KEGG: isc:IscW_ISCW022619 1.9e-34 glycine rich
secreted cement protein, putative K06237"
/codon_start=1
/product="nematode cuticle collagen domain protein"
/protein_id="KJH42956.1"
/db_xref="InterPro:IPR002486"
/db_xref="InterPro:IPR008160"
/translation="MFVVGFASTVALVTTIACLISIVIIVNDINDLRDDVLHTVGDFK
NVADNTWNQIIALHVHPTTDHDAPPIFASLFKRHKRNGFPPECNCGPLPENCPAGPPG
PPGSPGRPGFDGEDGSDGRPGVKGIALAVTQHIPGGCITCPAGPPGPPGARGNVGAPG
RDGERGCEGPQGEPGIPGPKGPTGEPGLAGIPGIAGRDGYPGAPGCRGLPGRPAPRGD
IGPKGYPGSDGKPGERGPPGERGPAGPSGRPGNRGNQGPPGADGIPGIPGRPGSDQGY
CPCPPHGRLRF"
CONTIG join(AZAF01012526.1:1..1269,gap(100),AZAF01012527.1:1..3413,
gap(100),AZAF01012528.1:1..26967,gap(100),AZAF01012529.1:1..1014,
gap(100),AZAF01012530.1:1..16147,gap(100),AZAF01012531.1:1..5414,
gap(408),AZAF01012532.1:1..7284,gap(100),AZAF01012533.1:1..2344,
gap(100),AZAF01012534.1:1..1206,gap(100),AZAF01012535.1:1..12506,
gap(100),AZAF01012536.1:1..992,gap(105),AZAF01012537.1:1..10957,
gap(137),AZAF01012538.1:1..6459)
//