LOCUS KK037140 249823 bp DNA linear CON 11-FEB-2014
DEFINITION Saccharomyces cerevisiae R103 chromosome XIII genomic scaffold
scaffold48, whole genome shotgun sequence.
ACCESSION KK037140 APIR01000000
VERSION KK037140.1
DBLINK BioProject: PRJNA162715
BioSample: SAMN01915093
KEYWORDS WGS.
SOURCE Saccharomyces cerevisiae R103
ORGANISM Saccharomyces cerevisiae R103
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 249823)
AUTHORS Treu,L., Toniolo,C., Nadai,C., Sardu,A., Giacomini,A., Corich,V.
and Campanaro,S.
TITLE The impact of genomic variability on gene expression in
environmental Saccharomyces cerevisiae strains
JOURNAL Environ. Microbiol. (2013) In press
PUBMED 24238297
REMARK Publication Status: Available-Online prior to print
REFERENCE 2 (bases 1 to 249823)
AUTHORS Campanaro,S., Treu,L. and Corich,V.
TITLE Direct Submission
JOURNAL Submitted (25-FEB-2013) Department of Biology, University of Padua,
Via U. Bassi 58/b, Padova, PD 35121, Italia
COMMENT ##Genome-Assembly-Data-START##
Assembly Method :: Newbler v. 2.3
Assembly Name :: R103
Genome Coverage :: 14.0x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..249823
/organism="Saccharomyces cerevisiae R103"
/mol_type="genomic DNA"
/submitter_seqid="scaffold48"
/strain="R103"
/isolation_source="grape bunch from Raboso Piave vineyard"
/db_xref="taxon:1182967"
/chromosome="XIII"
/country="Italy: Musile di Piave (VE)"
/collection_date="Oct-2006"
gene 1774..2871
/locus_tag="R103_M10006"
mRNA 1774..2871
/locus_tag="R103_M10006"
/product="hypothetical protein"
CDS 1774..2871
/locus_tag="R103_M10006"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94227.1"
/translation="MVLAKQWVLKNLPTPGEPFNFHFHDPACTFELIEKELSSEQLKD
GELLLETTYLSNDPAQKFWISSMDKNYAKGVQPGEIIPARGIGKVLASRNKAFSPGDY
VSAVTGWTTHAIISQENVQGLRKLDKNKVGKLWWYLSVLGGTSLTAYFIFFTYAQLQE
REEDYGKVYLISGAAGAVGTVCIQLALNVFKASKVIAIAGGPEKVAFVESFGDNVVGV
DYKDPSFKQKLIEAAGGENTVDYFIDNVGSNVLEAGVLLLKQRAMLIACGAISAYNDP
SKFVFKGYSFILTKRLVVKGVLVTDNIDDFPKALDKLGSLVKHGKIDLLKSATLEDGT
GDKFKNVPLIWKGLFSGVNKGKLITKVNNEE"
gene complement(3059..4750)
/gene="Ero1"
/locus_tag="R103_M10011"
mRNA complement(3059..4750)
/gene="Ero1"
/locus_tag="R103_M10011"
/product="Ero1p"
CDS complement(3059..4750)
/gene="Ero1"
/locus_tag="R103_M10011"
/note="Similar to YML130C Thiol oxidase required for
oxidative protein folding in the endoplasmic reticulum"
/codon_start=1
/product="Ero1p"
/protein_id="EWG94228.1"
/translation="MRLRTAIATLCLTAFTSATSNNSYIATDQTQNAFNDTHFCKVDR
NDHVSPSCNVTFNELNAINENIRDDLSALLKSDFFKYFRLDLYKQCSFWDANDGLCLN
RACSVDVVEDWDTLPEYWQPEILGSFNNDTMKEADDSDDECKFLDQLCQTSKKPVDIE
DTINYCDVNDFNGKNAVLIDLTANPERFTGYGGKQAGQIWSTIYQDNCFTIGETGESL
AKDAFYRLVSGFHASIGTHLSKEYLNTKTGKWEPNLDLFMARIGNFPDRVTNMYFNYA
VVAKALWKIQPYLPEFSFCDLVNKEIKNKMDNVISQLDTKIFNEDLVFANDLSLTLKD
EFRSRFKNVTKIMDCVQCDRCRLWGKIQTTGYATALKILFEINDADEFTKQHIVGKLT
KYELIALLQTFGRLSESIESVNMFEKMYGKRLNGSENRLSSFFQNNFFNILKEAGKSI
RYTIENINSTKEGKKKTNNSQSHVFDDLKMPKAEIVPRPSNGTVNKWKKAWNTEVNNV
LEAFRFIYRSYLDLPRNIWELSLMKVYKFWNKFIGVADYVSEETREPISYKLDIQ"
gene complement(6103..6315)
/gene="Cox14"
/locus_tag="R103_M10021"
mRNA complement(6103..6315)
/gene="Cox14"
/locus_tag="R103_M10021"
/product="Cox14p"
CDS complement(6103..6315)
/gene="Cox14"
/locus_tag="R103_M10021"
/note="Similar to YML129C 'Mitochondrial membrane protein,
involved in translational regulation of Cox1p and assembly
of cytochrome c oxidase (complex IV); associates with
complex IV assembly intermediates and complex III/complex
IV supercomplexes'"
/codon_start=1
/product="Cox14p"
/protein_id="EWG94229.1"
/translation="MSKYAWYTRVTDTLHRLTVLTLVGGTLYMSGGLAYTLYMNGKKY
EQQVTQQKALEEDNQQLQSPTAPPTE"
gene complement(6697..8238)
/gene="Msc1"
/locus_tag="R103_M10031"
mRNA complement(6697..8238)
/gene="Msc1"
/locus_tag="R103_M10031"
/product="Msc1p"
CDS complement(6697..8238)
/gene="Msc1"
/locus_tag="R103_M10031"
/note="Similar to YML128C 'Protein of unknown function;
mutant is defective in directing meiotic recombination
events to homologous chromatids; the authentic, non-tagged
protein is detected in highly purified mitochondria and is
phosphorylated'"
/codon_start=1
/product="Msc1p"
/protein_id="EWG94230.1"
/translation="MKQFKLVNAVSASFVLIGLVLANSDSVFDKWTQEDLADYLRDNK
KSLEKYATDSIEDLKKEASQVWDKHAQPKPWWQVWSSDSSSVSNSNSGWFGYTGSSDH
PVSDWLFDTWSTDSLRNFLKKNGVDVDDAKASKDSLVKTAKENFNKISKSLKSSGYYP
SSSYFDSWSTKDLQNWLNDNGIDYDKAVQSKDELVQKVKENIYRTSEKAEQQRLGLLE
SLDLAHQQILDTSGQIKDTVFDKWSSDQLTNWLESHKVNIDKNMAKKHDYLVRMAKEN
SANLKDDIYWYLDYMKRESSPFLTKTPEYVGSVWDSSKNFLTNLYSKFRGKTDNVIND
TFLVGLDSWPKDKLKMFLDARGIKYSMLSTEHQLRELVKKSRNEKLKILPKDYQKYFD
NSNWSLDDIKGWFADKKDDFQDSQTYSTIMQDFDKVSKNTNDAKDQIAKTWSNTFQSW
SQEDLLQYLKSFGVPVKQTSTKDDLINLAKQNTQWLFGTVKEPAYKRYLHNVKNWSKS
ILGFN"
gene 8626..10372
/gene="Rsc9"
/locus_tag="R103_M10036"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(10620..12095)
/gene="Erg13"
/locus_tag="R103_M10041"
mRNA complement(10620..12095)
/gene="Erg13"
/locus_tag="R103_M10041"
/product="Erg13p"
CDS complement(10620..12095)
/gene="Erg13"
/locus_tag="R103_M10041"
/note="Similar to YML126C '3-hydroxy-3-methylglutaryl-CoA
(HMG-CoA) synthase, catalyzes the formation of HMG-CoA
from acetyl-CoA and acetoacetyl-CoA; involved in the
second step in mevalonate biosynthesis'"
/codon_start=1
/product="Erg13p"
/protein_id="EWG94231.1"
/translation="MKLSTKLCWCGIKGRLRPQKQQQLHNTNLQMTELKKQKTAEQKT
RPQNVGIKGIQIYIPTQCVNQSELEKFDGVSQGKYTIGLGQTNMSFVNDREDIYSMSL
TVLSKLIKSYNIDTNKIGRLEVGTETLIDKSKSVKSVLMQLFGENTDVEGIDTLNACY
GGTNALFNSLNWIESNAWDGRDAIVVCGDIAIYDKGAARPTGGAGTVAMWIGPDAPIV
FDSVRASYMEHAYDFYKPDFTSEYPYVDGHFSLTCYVKALDQVYKSYSKKAISKGLIS
DPAGSDALNVLKYFDYNVFHVPTCKLVTKSYGRLLYNDFRANPQLFPEVDAELATRDY
DESLTDKNIEKTFVNVAKPFHKERVAQSLIVPTNTGNMYTASVYAAFASLLNYVGSDD
LQGKRVGLFSYGSGLAASLYSCKIVGDVQHIIKELDITNKLAKRITETPKDYEAAIEL
RENAHLKKNFKPQGSIEHLQSGVYYLTNIDDKFRRSYDVKK"
gene complement(12321..13259)
/gene="Pga3"
/locus_tag="R103_M10046"
mRNA complement(12321..13259)
/gene="Pga3"
/locus_tag="R103_M10046"
/product="Pga3p"
CDS complement(12321..13259)
/gene="Pga3"
/locus_tag="R103_M10046"
/note="Similar to YML125C 'Putative cytochrome b5
reductase, localized to the plasma membrane; may be
involved in regulation of lifespan; required for
maturation of Gas1p and Pho8p, proposed to be involved in
protein trafficking'"
/codon_start=1
/product="Pga3p"
/protein_id="EWG94232.1"
/translation="MSKEDIEGTNILDEPVHGIYIPAALFVVGVAITTYMSGELKILW
SLPILFMIIFVRAYSAYKRRRSLYPDRWTSLELEDQTIISKNTALYRFKLKTRLESLD
IPAGHHVAVRVPIDGKQEVRYYNPISSKLESGYLDLVVKAYVDGKVSKYFAGLNSGDT
VDFKGPIGTLNYEPNSSKHLGIVAGGSGITPVLQILNEIITVPEDLTKVSLLYANETE
NDILLKDELDEMAEKYPHFQVHYVVHYPSDRWTGDVGYITKDQMNRYLPEYSEDNRLL
ICGPDGMNNLALQYAKELGWKVNSTRSSGDDQVFVF"
gene complement(13720..15261)
/gene="Tub3"
/locus_tag="R103_M10056"
mRNA complement(join(13720..14939,15237..15261))
/gene="Tub3"
/locus_tag="R103_M10056"
/product="Tub3p"
CDS complement(join(13720..14939,15237..15261))
/gene="Tub3"
/locus_tag="R103_M10056"
/note="Similar to YML124C 'Alpha-tubulin; associates with
beta-tubulin (Tub2p) to form tubulin dimer, which
polymerizes to form microtubules; expressed at lower level
than Tub1p'"
/codon_start=1
/product="Tub3p"
/protein_id="EWG94233.1"
/translation="MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPK
GGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAAN
NYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSY
EYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNL
GISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPI
LSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVE
QVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCSCLHAIQHHCHRGRL
EENRSEIRPDVCQTCFRPLVCR"
gene complement(15615..17378)
/gene="Pho84"
/locus_tag="R103_M10066"
mRNA complement(15615..17378)
/gene="Pho84"
/locus_tag="R103_M10066"
/product="Pho84p"
CDS complement(15615..17378)
/gene="Pho84"
/locus_tag="R103_M10066"
/note="Similar to YML123C 'High-affinity inorganic
phosphate (Pi) transporter and low-affinity manganese
transporter; regulated by Pho4p and Spt7p; mutation
confers resistance to arsenate; exit from the ER during
maturation requires Pho86p'"
/codon_start=1
/product="Pho84p"
/protein_id="EWG94234.1"
/translation="MSSVNKDTIHVAERSLHKEHLTEGGNMAFHNHLNDFAHIEDPLE
RRRLALESIDNEGFGWQQVKTISIAGVGFLTDSYDIFAINLGITMMSYVYWHGSMPGP
SQTLLKVSTSVGTVIGQFGFGTLADIVGRKRIYGMELIIMIVCTILQTTVAHSPAINF
VAVLTFYRIVMGIGIGGDYPLSSIITSEFATTKWRGAIMGAVFANQAWGQISGGIIAL
ILVAAYKGELEYANSGAECDARCQKACDQMWRILIGLGTVPGLACLYFRLTIPESPRY
QLDVNAKLELAAAAQEQDGEKKIHDTSDEDMAINGLERASTAVESLDNHPPKASFKDF
CRHFGQWKYGKILLGTAGSWFTLDVAFYGLSLNSAVILQTIGYAGSKNVYKKLYDTAV
GNLILICAGSLPGYWVSVFTVDIIGRKPIQLAGFIILTALFCVIGFAYHKLGDHGLLA
LYVICQFFQNFGPNTTTFIVPGECFPTRYRSTAHGISAASGKVGAIIAQTALGTLIDH
NCARDGKPTNCWLPHVMEIFALFMLLGIFTTLLIPETKRKTLEEINELYHDEIDPATL
NFRNKNNDIESSSPSQLQHEA"
gene 18499..19431
/gene="Gtr1"
/locus_tag="R103_M10076"
mRNA 18499..19431
/gene="Gtr1"
/locus_tag="R103_M10076"
/product="Gtr1p"
CDS 18499..19431
/gene="Gtr1"
/locus_tag="R103_M10076"
/note="Similar to YML121W 'Cytoplasmic GTP binding protein
and negative regulator of the Ran/Tc4 GTPase cycle;
component of GSE complex, which is required for sorting of
Gap1p; involved in phosphate transport and telomeric
silencing; similar to human RagA and RagB'"
/codon_start=1
/product="Gtr1p"
/protein_id="EWG94235.1"
/translation="MSSNNRKKLLLMGRSGSGKSSMRSIIFSNYSAFDTRRLGATIDV
EHSHLRFLGNMTLNLWDCGGQDVFMENYFTKQKDHIFQMVQVLIHVFDVESTEVLKDI
EIFAKALKQLRKYSPDAKIFVLLHKMDLVQLDKREELFQIMMKNLSETSSEFGFPNLI
GFPTSIWDESLYKAWSQIVCSLIPNMSNHQSNLKKFKEIMNALEIILFERTTFLVICS
SNGENSNENHDSSDNNNVLLDPKRFEKISNIMKNFKQSCTKLKSGFKTLILNNNIYVS
ELSSNMVCFIVLKDMNIPQELVLENIKKAKEFFQ"
gene complement(19833..21374)
/gene="Ndi1"
/locus_tag="R103_M10081"
mRNA complement(19833..21374)
/gene="Ndi1"
/locus_tag="R103_M10081"
/product="Ndi1p"
CDS complement(19833..21374)
/gene="Ndi1"
/locus_tag="R103_M10081"
/note="Similar to YML120C 'NADH:ubiquinone oxidoreductase,
transfers electrons from NADH to ubiquinone in the
respiratory chain but does not pump protons, in contrast
to the higher eukaryotic multisubunit respiratory complex
I; phosphorylated; homolog of human AMID'"
/codon_start=1
/product="Ndi1p"
/protein_id="EWG94236.1"
/translation="MLSKNLYSNKRLLTSTNTLVRFASTRSTGVENSGAGPTSFKTMK
VIDPQHSDKPNVLILGSGWGAISFLKHIDTKKYNVSIISPRSYFLFTPLLPSAPVGTV
DEKSIIEPIVNFALKKKGNVTYYEAEATSINPDRNTVTIKSLSAVSQLYQPENHLGLH
QAEPAEIKYDYLISAVGAEPNTFGIPGVTDYGHFLKEIPNSLEIRRTFAANLEKANLL
PKGDPERRRLLSIVVVGGGPTGVEAAGELQDYVHQDLRKFLPALAEEVQIHLVEALPI
VLNMFEKKLSSYAQSHLENTSIKVHLRTAVAKVEEKQLLAKTKHEDGKITEETIPYGT
LIWATGNKARPVITDLFKKIPEQNSSKRGLAVNDFLQVKGSNNIFAIGDNAFAGLPPT
AQVAHQEAEYLAKNFDKMAQIPNFQKNLSSRKDKIDLWFEENNFKPFKYNDLGALAYL
GSERAIATIRSGKRTFYTGGGLMTFYLWRILYLSMILSARSRLKVFFDWIKLAFFKRD
FFKGL"
gene 22178..23252
/locus_tag="R103_M10101"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 23901..25412
/gene="Ngl3"
/locus_tag="R103_M10121"
mRNA 23901..25412
/gene="Ngl3"
/locus_tag="R103_M10121"
/product="Ngl3p"
CDS 23901..25412
/gene="Ngl3"
/locus_tag="R103_M10121"
/note="Similar to YML118W 'Putative endonuclease, has a
domain similar to a magnesium-dependent endonuclease motif
in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p'"
/codon_start=1
/product="Ngl3p"
/protein_id="EWG94237.1"
/translation="MDSQVEGKISPSQKESSSTSGLVSPSEDGPAHQKIHRDQLSLDQ
IKKIREERAQKRQVRRNSLISQGKDPDFPTPDLQFIERPFLPINHDNSKGLTPATIQV
TQDSLDVKIMTYNTLAQTLIRRDFFPESGPALKWHKRSKVLVHELKKYRPDVVSLQEV
DYNELNFWQENFHKLGFDMIFKRHEGKTHGLLVAWNNKKFQLDNDWMLDYDNILAGNV
ISARTRTKNIALIISLYFKGITDSSSRGIIVANTHLFWHPFGVFERLRQSYLVLQKIQ
EIKACSKYNGWHSLLMGDFNTEPEEPPYLAITKRPLILKGPIRAMVECSLAYRYSKKR
NGEESDQDDEECDEKSRGEGHSDQPQNPKPESFTATKEEKALVNQLVALHNSLHVKGV
SLYGIGYGKVHPENANGSHGEPGLSNWANTWCGLLDYIFYIEGDHNQDTRQKEPLNAF
EGNNNVKIIGYLRMPCAQEMPKHSQPFEGEYASDHISLMCQIRLFFGGGGKST"
gene 25814..29218
/gene="Nab6"
/locus_tag="R103_M10131"
mRNA 25814..29218
/gene="Nab6"
/locus_tag="R103_M10131"
/product="Nab6p"
CDS 25814..29218
/gene="Nab6"
/locus_tag="R103_M10131"
/note="Similar to YML117W 'Putative RNA-binding protein
that associates with mRNAs encoding cell wall proteins in
high-throughput studies; deletion mutants display
increased sensitivity to some cell wall disrupting agents;
expression negatively regulated by cAMP'"
/codon_start=1
/product="Nab6p"
/protein_id="EWG94238.1"
/translation="MSNSNSKKPVANYAYRQQQDYNGMNAMVGNPMMYHPVDFVNGAG
QYGPSQHPAYYTNSPLPNIPPTPFDTAYGASLLPSHLLMGSPFVSSPNMQSGYNSARS
SNLKRKAYSRPVSNHNGYNGNSNSNQNNTNNGMVTPSNYYRVGRNSFSRNNNSTRNVT
HNNNKGCDTRNNSGRRTFTRNNIFDDINPEMLLQRPFCINYKVLPTGDDAYRTRSLLI
ENVDHSIDLHSIVKNFVKSNTLESAYLIEGGKSDDSKDVETKNLSILISFLTKGDCLN
FYNNILQRLSEFKTFLKSEALNLKFVCLNYDPKCLPTFIESEALTENAEEADITNGST
MISASLHHNIANKDATRSIIIEFKSPVEKSDLFKKKLQFLDRSKNKRYILESIDLVNT
DVPSNQFPENYAVLTFLNISMAIEVLDYLKKYSKNLGISKCFYVSLAPLVVSSARSSV
ANIYEGKTSTHRLSVPSVTAGNNNDSNNNGNNNKSNMSGITTLNNNSSIGVSVYGHSN
MSLTSLSSSVSLNEEIDMLATKLQGVELDGTYLEINYRDYQTPTIEEHSTHLSNVKIS
KTTENSRQFSQDIPSPLPLNEHMFMNDSNQSNGAIIPQQLIATPSPVSPNLQMNQRVL
PNPITQSLEQNFNVSAKVASSMGSDIGNRTIYIGNINPRSKAEDICNVVRGGILQSIK
YIPEKKICFVTFIEAPSAVQFYANSFIDPIVLHGNMLRVGWGHYSGPLPKSISLAVTI
GASRNVYVSLPEFAFKEKFIHDPQYKKLHETLSLPDAEQLREDFSTYGDIEQINYLSD
SHCCWINFMNISSAISLVEEMNKESTVQNESGEVTLKRATEEKFGGRYKGLLINYGKD
RCGNINKNLIAGKNSRFYKKVKRPSYNIRLSKLEEKRRQNEIDEKEKAFDKPLNLESL
GISLDAHKDNGGGETGTANNTGHENESELEAENENGNETGSFGGLGLAVASSDVKRAT
SDETDYEDIFNKSSGSSDSSSDVEVIMHSPSDPEYALKSQTLRSSSQTVINSKRPVKI
EDEEEAVGMSQLNYRSSLRQAPPRAPSTLSYNHSKNNETPMQDILTNGETANNRKKKR
GSFPRHRTIPGSDVMAQYLAQVQHSTFMYAANILGASAEDNTHPDE"
gene 29043..29345
/locus_tag="R103_M10136"
mRNA 29043..29345
/locus_tag="R103_M10136"
/product="hypothetical protein"
CDS 29043..29345
/locus_tag="R103_M10136"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94239.1"
/translation="MAKQQITERRREDLFQGIERYQDLTSWPNTLHKCNIRHLCMQPI
FWVPLRKTTRILTSNIAVLSLKYNAYPSDYLDKAQGSLRRPAKVFLPFFLRFLCIN"
gene 29814..31442
/gene="Atr1"
/locus_tag="R103_M10141"
mRNA 29814..31442
/gene="Atr1"
/locus_tag="R103_M10141"
/product="Atr1p"
CDS 29814..31442
/gene="Atr1"
/locus_tag="R103_M10141"
/note="Similar to YML116W Multidrug efflux pump of the
major facilitator superfamily, required for resistance to
aminotriazole and 4-nitroquinoline-N-oxide"
/codon_start=1
/product="Atr1p"
/protein_id="EWG94240.1"
/translation="MGNQSLVVLTESKGEYENETELPVKKSSRDNNIGESLTATAFTQ
SEDEMVDSNQKWQNPNYFKYAWQEYLFIFTCMISQLLNQAGTTQTLSIMNILSDSFGS
EGNSKSWLMASFPLVSGSFILISGRLGDIYGLKKMLLVGYVLVIIWSLICGITKYSGS
DTFFIISRAFQGLGIAFVLPNVLGIIGNIYVGGTFRKNIVISFVGAMAPIGATLGCLF
AGLIGTEDPKQWPWAFYAYSIAAFINFVLSIYAIPSTIPTNIHHFSMDWIGSVLGVIG
LILLNFVWNQAPISGWNQAYIIVILIISVIFLVVFIIYEIRFAKAPLLPRAVIKDRHM
IQIMLALFFGWGSFGIFTFYYFQFQLNIRQYTALWAGGTYFMFLIWGIIAALLVGFTI
KNVSPSVFLFFSMVAFNVGSIMASVTPVHETYFRTQLGTMIILSFGMDLSFPASSIIF
SDNLPMEYQGMAGSLVNTVVNYSMSLCLGMGATVETQVNSDGKHLLKGYRGAQYLGIG
LASLACMISGLYMVESFIKGRRARAAAEYDCTVA"
gene complement(31805..33412)
/gene="Van1"
/locus_tag="R103_M10151"
mRNA complement(31805..33412)
/gene="Van1"
/locus_tag="R103_M10151"
/product="Van1p"
CDS complement(31805..33412)
/gene="Van1"
/locus_tag="R103_M10151"
/note="Similar to YML115C 'Component of the mannan
polymerase I, which contains Van1p and Mnn9p and is
involved in the first steps of mannan synthesis; mutants
are vanadate-resistant'"
/codon_start=1
/product="Van1p"
/protein_id="EWG94241.1"
/translation="MGMFFNLRSNIKKKAMDNGLSLPISRNGSSNNIKDKRSEHNSNS
LKGKYRYQPRSTPSKFQLTVSITSLIIIAVLSLYLFISFLSGMGIGVSTQNGRSLLGS
SKSSENYKTIDLEDEEYYDYDFEDIDPEVISKFDDGVQHYLISQFGSEVLTPKDDEKY
QRELNMLFDSTVEEYDLSNFEGAPNGLETRDHILLCIPLRNAADVLPLMFKHLMNLTY
PHELIDLAFLVSDCSEGDTTLDALIAYSRQLQNGTLSQIFQEIDAVIDSQTKGTDKLY
LKYMDEGYINRVHQAFSPPFHENYDKPFRSVQIFQKDFGQVIGQGFSDRHAVKVQGIR
RKLMGRARNWLTANALKPYHSWVYWRDADVELCPGSVIQDLMSKNYDVIVPNVWRPLP
TFLGTEQPYDLNSWMESQEALALAKTLDEDDVIVEGYAEYPTWRVHLAYIRDAEGDPN
EAVDLDGVGGVSILAKAKIFRNGVQFPAFTFENHAETEAFGKMAKKMGYRVGGLPHYT
IWHIYEPSDDDLKEIASREREKRRQSE"
gene complement(33659..35191)
/gene="Taf8"
/locus_tag="R103_M10161"
mRNA complement(33659..35191)
/gene="Taf8"
/locus_tag="R103_M10161"
/product="Taf8p"
CDS complement(33659..35191)
/gene="Taf8"
/locus_tag="R103_M10161"
/note="Similar to YML114C TFIID subunit (65 kDa), involved
in RNA polymerase II transcription initiation"
/codon_start=1
/product="Taf8p"
/protein_id="EWG94242.1"
/translation="MTSKTSESGTGTQSTIVQLRNLPDLTEISHLEIDAPVVEILKKT
VLFQLNSLNICISNFALDELVNLVTVQMDGMFRNLHNLTLLQRRSQASQADLKLLLRE
FNLDAPSLYQQFQASEFIKSKHSTEYEKLMSWSSPAALPHNEEDEEDELNNIEEQQNE
INVLLPPSNPLEKQIPSWLPNFPPDHTYKFTPEFNHPITDLKTIKKEIVKESQESEKA
LLNLNKSLSHISSASNTPQPPGLDDEDAIEQQLEIWGNALEERKPTITEKSFNENNIE
QYAKYRVELARERVTKFEVNQLKRTKNPFLKISETLYLPESPHQSHKTIQKTIELQFR
KSMTLFMHNLPKVQKLKKEKIRMAKEERAKSLKRRQEELISQRTKREQDEGHDLELLL
NNEHARDAADDTTTPNALNNSTIVINTNAEDEDDDINLFGILGSSEDENEMSSMPAEN
LVAESEPPTITAQDTTNTTPVAHNTTNIDATTSHSPHSTPNENAPTSPPADIATDHDI
TM"
assembly_gap 35525..35648
/estimated_length=124
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 35662..36399
/gene="Dat1"
/locus_tag="R103_M10166"
mRNA 35662..36399
/gene="Dat1"
/locus_tag="R103_M10166"
/product="Dat1p"
CDS 35662..36399
/gene="Dat1"
/locus_tag="R103_M10166"
/note="Similar to YML113W 'DNA binding protein that
recognizes oligo(dA).oligo(dT) tracts; Arg side chain in
its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is
required for DNA-binding; not essential for viability'"
/codon_start=1
/product="Dat1p"
/protein_id="EWG94243.1"
/translation="MAKTLAQGRKPGSGRKPGKGKTLREGRKPGSGRRRRQDTGGKET
DGSQQDQESRLISSRDMEAVDALRELTHSPSSHSAHNSSAAPPPHAAAASTSLPPSLD
YTHQSFMDQQQQQQQQQLLQQQRVDVVPPKPFITHKILLSSTGNSGGHVNSNYNADHS
INHNSNHNLNSNVNVNMNFTINGSNQDPSSSFLMGPYNYLQRPFIVKPYLDLSTSTAA
SNQPRTQPSPAAHITKNSDSTEKNATI"
gene 36671..37561
/gene="Ctk3"
/locus_tag="R103_M10171"
mRNA 36671..37561
/gene="Ctk3"
/locus_tag="R103_M10171"
/product="Ctk3p"
CDS 36671..37561
/gene="Ctk3"
/locus_tag="R103_M10171"
/note="Similar to YML112W Gamma subunit of C-terminal
domain kinase I (CTDK-I), which phosphorylates both RNA
pol II subunit Rpo21p to affect transcription and pre-mRNA
3' end processing, and ribosomal protein Rps2p to increase
translational fidelity"
/codon_start=1
/product="Ctk3p"
/protein_id="EWG94244.1"
/translation="MDSLEARLQFIQVLKNLQKTLHKTRDSITSSSTTTPPSSQQKLN
NDPIQFYLRNYRHHYEDFHQCLFDTTMKMDPLDRLDVVIYYVRIIRNLYPHSHSNTNV
TKVLNEVLLMDIDLVFELCLPCQDWKSLTNQATCKELFLDLSKLIHYDATSVTHTPSD
TTLIDATTWYSVKTERTTKDYKESLQRTESLLKDRDLKKLAFFQQFNSDTTAINPDLQ
TQPTNANILLHRMEADRELHKRSKETSWYIERPSNDILDESEFKSLWTHFETTDSGFD
KDDYKNIKALNDIAKASYMY"
gene 38556..41318
/gene="Bul2"
/locus_tag="R103_M10181"
mRNA 38556..41318
/gene="Bul2"
/locus_tag="R103_M10181"
/product="Bul2p"
CDS 38556..41318
/gene="Bul2"
/locus_tag="R103_M10181"
/note="Similar to YML111W Component of the Rsp5p
E3-ubiquitin ligase complex, involved in intracellular
amino acid permease sorting, functions in heat shock
element mediated gene expression, essential for growth in
stress conditions, functional homolog of BUL1"
/codon_start=1
/product="Bul2p"
/protein_id="EWG94245.1"
/translation="MTFTFSTSSRKNGRPPLKSVSTEDNIHLLRKRRQQQLSSNSTDN
SLHPNSGQTPRASDSQDDDIRSASTTNLDRLRQEREENSLEMDCTQSRLSHRANMLVD
VLPSFEMYNALHRHIPQGNVDPDRHDFPPSYQEVRTQRMTILPSNDNSVERSQLTAVP
GSENACNNATAHSLTNLHPLQTQHLTINSTRSGGQSLHSSSDTNISQIPFEDDLNDSD
NIFIDKLYTLPKLSTPIEIDIRITKTASIPHERPEEQSILKEYTSGDIIHGYCLIENR
SSQPLKFEMFYVTLEAYISVIDRQKGKRTLKRFLRMVDLSASWSYTNITPSTGINIVP
GERDFDDAIIGLSNSRELKPNTKYKKFFMFKLPTQLLDVTCKQEQFSHCLLPPSFGID
KYKNNCKYSGIKVNSVLGCGHLGTKGSPILTLDMADDNLSINYTIDAKIVGKDKRTSK
LNIMKEKEYNLRVMPFPFAGVTNQQNEKTCLRQLKNLESLIEDRFEALNKIFKKLELN
EAISNVDIHDTDISGTLDGNEDLDSDEILRRKLDQLHINNRIDDTASQSPSYDSKNMA
PKENLVETELRYKFKNKNKSNSSLFSHFLSSSETGSSSTGPHVYNSGLIVLSVKKPQS
ALPYWSPSLLRKTNKFEAKSEQEKENWQRLMGMLPEGVKTPLTKLDVHLTCIQSNNSA
GHKPPEISSVTTEFVVITAKSDNSIPIKFCTELLMNENRLNKLKTKFLTYQKKVHEYR
KKFEENHAKLNELYNRNRDHFTPKELLFTNFISDQINNDIDSLAGLKVNIIDLHDIFK
KQIHTFEEENEDIISKKGSSNPPSASSSNNNFLQATFSNGASTATKFTQQIVHEWEKV
KPLQYKRDVTVNLKLNPNIKETLVPNFETCLCCRFYCVRVNIKFDNHLGSMKVDIPVD
VKKLQI"
gene complement(41647..42570)
/gene="Coq5"
/locus_tag="R103_M10186"
mRNA complement(41647..42570)
/gene="Coq5"
/locus_tag="R103_M10186"
/product="Coq5p"
CDS complement(41647..42570)
/gene="Coq5"
/locus_tag="R103_M10186"
/note="Similar to YML110C
'2-hexaprenyl-6-methoxy-1,4-benzoquinone
methyltransferase, involved in ubiquinone (Coenzyme Q)
biosynthesis; localizes to the matrix face of the
mitochondrial inner membrane in a large complex with other
ubiquinone biosynthetic enzymes'"
/codon_start=1
/product="Coq5p"
/protein_id="EWG94246.1"
/translation="MLISSRIVRSSLVNVPLRLSRFFTQAHRACKEEEVNSPLSSTAE
QPEQKYTHFGSKTVLKSTKQKLVGDVFSSVANRYDLMNDVMSLGIHRLWKDHFINKLD
AGKRPNSTTPLNFIDVAGGSGDIAFGLLDHAESKFGDTESTMDIVDINPDMLKEGEKR
AMEQGKYFKDPRVRFLVSNGEKLEEIDSDSKDIYTVSFGIRNFTDIQKGLNTAYRVLK
PGGIFYCLEFSKIENPLMDFAYQQWAKVLPVMGSMIANDYDSYQYLVESIERFPDQET
FKSMIEKAGFKSAGYESLTFGICAIHWGIKV"
gene 43256..46087
/gene="Zds2"
/locus_tag="R103_M10191"
mRNA 43256..46087
/gene="Zds2"
/locus_tag="R103_M10191"
/product="Zds2p"
CDS 43256..46087
/gene="Zds2"
/locus_tag="R103_M10191"
/note="Similar to YML109W 'Protein with a role in
regulating Swe1p-dependent polarized growth; interacts
with Cdc55p; interacts with silencing proteins at the
telomere; implicated in the mitotic exit network through
regulation of Cdc14p localization; paralog of Zds1p'"
/codon_start=1
/product="Zds2p"
/protein_id="EWG94247.1"
/translation="MVLMEDVQNKDGHNTVENSSGGTDSNNNIQMRRMRKTQLSKKEL
FEKRKSDVLIAAKSLDTEIQNVKNLKRLSIGSMDLVIDPELEFKVNSRNSYSSDSSKE
SLQESLHEENIIRSEQKEEQGSEDNDAYEEGDATNVDDSIDITQTEYLHDEETLEKEK
IIRNASSSTSSSARVTSRNRRLSGVKTLAHDVVLDVENDHDSKMVDLTQNLLWVPADQ
HPNVKPENYLELIQDTLQNIQISTNQDIDENKLELGNNHVISNRKRTGSVVRRPSRLK
TSYTKFDDEPPLADKPQEGEIQVDKRISSSDIKTIRSVSLKEITEELTKISNNAGLTD
SDAVTLARSLSMSGSFTNESLHLNGNHTENDNEFASNMFNETGLTIPERSSLRRSKFN
TYKIRLEGSSLPQAVKLNSLMNIQTNDNRRSASSPASYTQVPQEQASLNDFHEIFDHY
RRTSTDWSTENEKYVDSTNYYSDEEDLTHASISQESSLLSTDSSNNSVLIKPHNTGSM
ISEKLDQHVSSSEKSNTNNSEPNHGWSWLNSSNGSLNGNEQTYQQLTDDEDDEECVDN
EKADFVNLSVSRRAKSTKRASERINHSKNRHSPIFQIHSEEAKSVVITPSVVSSSESQ
PSKPTAPAAVEKKVELPTDTQASTHKKNSLEKRLAKLFKRKQHSGTCKSDVKVIKKSV
KKELKKKASHSSLSKFRKSPKKKPQEAEVNCERPSSPTKTITEDIDTESVIEPEVRSS
NASTLLPDSHTSHSSEFVVETISELDGDDSFDISGGDVNYDVEVHSSISRDTTAGLEE
DIGAEREDNTSPTAPQISTLPPRKLTFEDVVKPDYPNAPIKFTDSAFGFPLPMITNST
VIMFDHRLGINVERAIYRLSHLKLSDPGRELRQQVLLSNFMYSYLNLVNHTLYMEQVG
TGDIAFNGDSALGMMDKNDSDGTILIPDI"
gene 46412..46729
/locus_tag="R103_M10196"
mRNA 46412..46729
/locus_tag="R103_M10196"
/product="hypothetical protein"
CDS 46412..46729
/locus_tag="R103_M10196"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94248.1"
/translation="MSKSNTYRMLVLLEDDTKINKEDEKFLKGKPGKMHEFVDELVLP
FNVDELDELNTWFDKFDAEICIPNEGHIKYEISSDGLIVLMLDKEIEEVVEKVKKFVD
ENN"
gene complement(46884..47888)
/gene="Pml39"
/locus_tag="R103_M10201"
mRNA complement(46884..47888)
/gene="Pml39"
/locus_tag="R103_M10201"
/product="Pml39p"
CDS complement(46884..47888)
/gene="Pml39"
/locus_tag="R103_M10201"
/note="Similar to YML107C 'Protein required for nuclear
retention of unspliced pre-mRNAs along with Mlp1p and
Pml1p; anchored to nuclear pore complex via Mlp1p and
Mlp2p; found with the subset of nuclear pores farthest
from the nucleolus; may interact with ribosomes'"
/codon_start=1
/product="Pml39p"
/protein_id="EWG94249.1"
/translation="MEKDALEVRLKSIRHSLDKNTKLLPGKYRNTLGERLITKWRYKK
KSHNGSSMLPEKCKSHVQLYDDLVQESSKHFVGFRLHDLRALLKRICSIQNYTRHVLI
EWDVRWVNPLTLASKGWEPYQSASQSQVPFKCCCCHAIMTIPLLKNGDDVADYNMKLN
EKIWNCNIIGNHLQKCPWRENQVDLNKEYYLSSQNLIREIERIHTEIDRIVSGSNEFS
LKRNSSRIFHYLSEKEIQKLAFFFDCKDYSLVGLLLLGYTKFQKDDLVQCTACFHRAS
LKKLEYTEFNGHALWCRYYNKELLPTMLLELIGKEDKLITKLGVGERLNKLEAVLQTL
"
gene 48391..49071
/gene="Ura5"
/locus_tag="R103_M10211"
mRNA 48391..49071
/gene="Ura5"
/locus_tag="R103_M10211"
/product="Ura5p"
CDS 48391..49071
/gene="Ura5"
/locus_tag="R103_M10211"
/note="Similar to YML106W 'Major orotate
phosphoribosyltransferase (OPRTase) isozyme that catalyzes
the fifth enzymatic step in de novo biosynthesis of
pyrimidines, converting orotate into
orotidine-5'-phosphate; minor OPRTase encoded by URA10'"
/codon_start=1
/product="Ura5p"
/protein_id="EWG94250.1"
/translation="MPIMLEDYQKNFLELAIECQALRFGSFKLKSGRESPYFFNLGLF
NTGKLLSNLATAYAIAIIQSDLKFDVIFGPAYKGIPLAAIVCVKLAEIGGSKFQNIQY
AFNRKEAKDHGEGGIIVGSALENKRILIIDDVMTAGTAINEAFEIISNAKGQVVGSII
ALDRQEVVSTDDKEGLSATQTVSKKYGIPVLSIVSLIHIITYLEGRITAEEKSKIEQY
LQTYGASA"
gene complement(49484..50305)
/gene="Sec65"
/locus_tag="R103_M10216"
mRNA complement(49484..50305)
/gene="Sec65"
/locus_tag="R103_M10216"
/product="Sec65p"
CDS complement(49484..50305)
/gene="Sec65"
/locus_tag="R103_M10216"
/note="Similar to YML105C 'Subunit of the signal
recognition particle (SRP), involved in protein targeting
to the ER; interacts with Srp54p; homolog of mammalian
SRP19'"
/codon_start=1
/product="Sec65p"
/protein_id="EWG94251.1"
/translation="MPRLEEIDDFNDIDDLDMELAELDPSLRTPIAPKITPKVVRSQD
QENPAFLPGTNNNSNSNNNSSNEKEQLSFINPKTGKVERSEAISKKDLEEVKRFQVLY
PCYFDINRSHKEGRRVPKELAVENPLAKTMADAVRELGILCIFEGEKCHPQDFGNPGR
IRVLFKENGQLIGAATKFKGGKRQLMKAVGEYMKRHPTTIESLREIPYGPDFDNIEFK
KIPRVKGFKMNEIVPLHSPFLMGHPMTKSVYETPKITAAEKSFKPPKNKYKVVRR"
gene complement(50556..53939)
/gene="Mdm1"
/locus_tag="R103_M10221"
mRNA complement(50556..53939)
/gene="Mdm1"
/locus_tag="R103_M10221"
/product="Mdm1p"
CDS complement(50556..53939)
/gene="Mdm1"
/locus_tag="R103_M10221"
/note="Similar to YML104C 'Intermediate filament protein,
required for nuclear and mitochondrial transmission to
daughter buds; contains a Phox homology (PX) domain and
specifically binds phosphatidylinositol 3-phosphate
(PtdIns-3-P)'"
/codon_start=1
/product="Mdm1p"
/protein_id="EWG94252.1"
/translation="MPKFPQFRLILVLFYLISMIQWSVITFSLGFFLNVCIFAYFVFF
KSLPDLPKPQPRFVDIVPESSNTVDVDKELKSVEGLIQDGNAQIGKELESIVNLIIKD
FVEPWFTKIDKNSDAEFLKVIKWRLLQTLLVVKDKLMKNDSASLIVLKLLPIFNKHFS
TFCDAREAVLSDLTLERHKSANIDLQIAVEFNKNYKIHKSLSLKPNALQKEIEKSIRK
TVIGLLPHLFDNDELDSLLVFTLMTEVLTTCIISPLIFKFTDPDSWNLRIVSLSQNYF
EEKHKVHKIRRMLSKELQDHKKVMNDVANKDVGELSSEKLELNAEYTGKQFEHYLNQL
DSLLDLSDIKYVAYSLALKIYQLKENEHLTKENLKYKKRLLLSLNLIESKLSVPGSEI
DTASKKLAREANYPDLNMDNGIVLKEMASFLTSITLKDIVDDSEFLPFFESFLGSVPE
TQGSTFLEYSQTIESFKNPLEDATSEDIISGYSGISTMQLQEISSKFFHNNNLQNMKL
LDEGLVKNIILFRNSFQINNDEDTFILARKSVLLLQTEAIKYLDDRFLPLFKKTPSFL
KMLSTSHIISTDIYAHFLSRIGGVNNPEQNKIIKDNVKTDFMNPVRIFANPGITDALD
NIVNGSESKPDKSRISSNPRYSQLFGSENDNIFKDKLFDDENDNTSEISVVEDQLDHP
RNMEKVSVSSGNSGLNPSQFYGSNNFRDNIASLTISIDQIEKELELLRHLILKADLTN
NQMQLKILKKSQRTLLKELEMKELLKQQYMVRENGNSLFRKTKIYIRSYFSENSSNGL
KEITYYIINIHHFNNGQVSSWDMARRYNEFFELNTYLKKNFRDLMRQLQDLFPSKVKM
SLKYHVTKTLLYEERKQKLEKYLRELLSISEICEDNIFRRFLIDPTPFKLNKEYMHDD
ILEEPLHEPIGSSNSTSNSSSVVDLQSSEDGGELNFYEDERHFFTDSGYPFYSQNKSF
VKQICDLFISLFALNKANAGWLRGRAIITVLQQLLGSTIEKYIKVSIQKLRSEDQVFE
AIVTFKNMLWGDNGLFERKRNETAEATRSEGEKLRTEQLALTSLQRLFADTCGRVVGL
RDSHEAAGRVHAMLQNPYLNASLLLEALDAILLDIICND"
gene complement(54199..59160)
/gene="Nup188"
/locus_tag="R103_M10226"
mRNA complement(54199..59160)
/gene="Nup188"
/locus_tag="R103_M10226"
/product="Nup188p"
CDS complement(54199..59160)
/gene="Nup188"
/locus_tag="R103_M10226"
/note="Similar to YML103C Subunit of the nuclear pore
complex (NPC), involved in the structural organization of
the complex and of the nuclear envelope, also involved in
nuclear envelope permeability, interacts with Pom152p and
Nic96p"
/codon_start=1
/product="Nup188p"
/protein_id="EWG94253.1"
/translation="MATPSFGNSSPQLTFTHVANFMNDAAADVSAVDAKQLAQIRQFL
KANKTNLIESLNTIRQNVTSSGDHNKLRSTIANLLQINVDNDPFFAQSEDLSHAVEFF
MSERSSRLHIVYSLLVNPDIDLETYSFIDNDRFNVVGKLISIISSVIQNYDIITASSL
AHDYNNDQDMFTIVSLVQLKKFSDLKFILQILQILNLMILNTKVPVDIVNQWFLQYQN
QFVEFCRNINSTDKSIDTSSLQLYNFQNFQDLSYLSETLISRISSLFTITTILILGLN
TSIAQFDIQSPLYMDTETFDTVNSALENDVATNIVNEDPIFHPMIHYSWSFILYYRRA
LQSSESFDDSDITKFALFAESHDVLQKLNTLSEILSFDPVYTTVITVFLEFSLNFIPI
TASTSRVFAKIISKAPEQFIENFLTNDTFEKKLSIIKAKLPLLNESLIPLINLALIDT
EFANFELKDICSFAVTKSSLNDLDYDLIADTITNSSSSSDIIVPDLIELKSDLLVAPP
LENENSNCLLSIPKSTKGKILTIKQQQQQQQNGQQPPTTSNLIIFLYKFNGWSLVGRI
LQNLLHSYMEKGTQLDDLQHELMISIIKLVTNVVDPKTSIEKSSEILSYLSNSLDTSA
STINGASIIQVIFEIFEISLQRKDYTSIVQCCEFMTMLTPNYLHLVSSYLNKSDLLDK
YGKTGLSNMILGSVELSTGDYTFTIQLLKLTKVFIRESLSLKNIHISKRSKIDIINKL
ILHAIHIFESYYNWKYNNFLQKFEIAFHLTLIFYDVLHDVFTINPHQKDQLIISSSAN
KLLQLFLTPMDSIDLAPNTLTNILISPLNTTTKILGDKILGNLYSKVMNNSFKLCTLL
ISIRGSNRDLKPSNLEKLLFINSSKLVDVYTLPSYVHFKVQIIELLSYLVEAPWNDDY
PFLLSFLGEAKSMAFLKEVLSDLSSPVQDWNLLRSLYIFFTTLLESKQDGLSILFLTG
QFASNKKINDESSIDKKSSILTVLQKNSLLLDSTPEEVSCKLLETITYVLNTWTNSKI
FIKDPKFVNSLLAKLKDSKKLFQKKENLTRDETVSLIKKYKLISRIVEIFALCIYNST
DSNSEILNFLNQEDLFELVHHFFQIDGFNKTFHDELNLKFKEKWPSLELQSFQKIPLS
RINENENFGYDIPLLDIVLKADRSWNEPSKSQTNFKEEITDASLNLQYVNYEISTAKA
WGALITTFVKRSTVPLNDGFVDLVEHFLKLNIDFGSDKQMFTQIYLERIELSFYILYS
FKLSGKLLKEEKIIELMNKIFTIFKSGEIDFIKNIGKSLKNNFYRPLLRSVLVLLELV
SSGDRFIELISDQLLEFFELVFSKGVYLILSEILCQINKCSTRGLSTDHTTQIVNLED
NTQDLLLLLSLFKKITNLNPSKNFNVILASSLNEVGTLKVILNLYSSAHLIRINDEPI
LGQITLTFISELCSIEPIAAKLINSGLYSVLLESPLSVAIQQGDIKPEFSPRLHNIWS
NGLLSIVLLLLSQFGIKVLPETCLFVSYFGKQIKSTIYNWGDNKLAVSSSLIKETNQL
VLLQKMLNLLNYQELFIQPKNSDDQQEAVELVIGLDSEHDKKRLSAALSKFLTHPKYL
NSRIIPTTLEEQQQLEDESSRLEFVKGISRDIKALQDSLFKDV"
gene complement(59379..59549)
/locus_tag="R103_M10231"
ncRNA complement(59379..59549)
/ncRNA_class="snoRNA"
/locus_tag="R103_M10231"
/product="SNR85"
gene 59905..61311
/gene="Cac2"
/locus_tag="R103_M10236"
mRNA 59905..61311
/gene="Cac2"
/locus_tag="R103_M10236"
/product="Cac2p"
CDS 59905..61311
/gene="Cac2"
/locus_tag="R103_M10236"
/note="Similar to YML102W Component of the chromatin
assembly complex (with Rlf2p and Msi1p) that assembles
newly synthesized histones onto recently replicated DNA,
required for building functional kinetochores, conserved
from yeast to humans"
/codon_start=1
/product="Cac2p"
/protein_id="EWG94254.1"
/translation="MEASHLQIYWHDSQPVYSLTFQKNSANDKLFTAGGDNKVRIWKL
NRDENGQNGGVRKIESLDFLGSLTHHEQAINVIRFNSKGDVLASAGDDGQVLLWKQED
PNTQQESVVRPFGMDAETSEADENKEKWVVWKRLRGGSGATAAAEIYDLAWSPDNRNI
VVACMDNSIRLFDVGAGTLVCGQSDHGHYVQGVAWDPLNQFILSQSADRSLHVYGVIL
SSAGVVTGLKLRSKIAKAELPCPGDVLRTNYLFHNETLPSFFRRCSISPCGGLVVVPS
GVYKVAGDEVANCVYVYTRSGILNSAGGVKNRPAIRIPSLKKPALMAAFSPVFYETCQ
KSVLKLPYKLVFAIATTNEVLVYDTDVLEPLCVVGNIHYSPITDLAWSEDGSTLLISS
TDGFCSYVSINTETQFGSRIEPPAMHAEPLDTDESAVAAKNQREAGGIVNMLPVKKIP
CNSSDSKKRRIHPTPVDL"
gene complement(61020..61337)
/locus_tag="R103_M10241"
mRNA complement(61020..61337)
/locus_tag="R103_M10241"
/product="hypothetical protein"
CDS complement(61020..61337)
/locus_tag="R103_M10241"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94255.1"
/translation="MYRIKYIKNHKSTGVGCMRLFLLSLLLQGIFFTGSMFTIPPASR
WFLAATALSSVSSGSACIAGGSILEPNCVSVLIDTYEQNPSVDEISRVEPSSDHAKSV
MGE"
gene complement(61346..61699)
/gene="Cue4"
/locus_tag="R103_M10246"
mRNA complement(61346..61699)
/gene="Cue4"
/locus_tag="R103_M10246"
/product="Cue4p"
CDS complement(61346..61699)
/gene="Cue4"
/locus_tag="R103_M10246"
/note="Similar to YML101C 'Protein of unknown function;
has a CUE domain that binds ubiquitin, which may
facilitate intramolecular monoubiquitination'"
/codon_start=1
/product="Cue4p"
/protein_id="EWG94256.1"
/translation="MDGSTIVFILTMVCLFVYTVKHRGAKQVPSRTVQDAKPAPSVAT
NDPSPEPVPSAPEERAARLNRHGSDRKRAVNSDMVEIVMTMAPHVPQEKVVQDLRNTG
SIEHTMENIFAGKLD"
gene 61749..61961
/locus_tag="R103_M10251"
mRNA 61749..61961
/locus_tag="R103_M10251"
/product="hypothetical protein"
CDS 61749..61961
/locus_tag="R103_M10251"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94257.1"
/translation="MYKCVPSYYSKAGTIVVTYCEISALLVSLKNRVQGKRGETEGQI
EISRKAGHPRPCILIWRIWDCLLESV"
gene 62236..65532
/gene="Tsl1"
/locus_tag="R103_M10256"
mRNA 62236..65532
/gene="Tsl1"
/locus_tag="R103_M10256"
/product="Tsl1p"
CDS 62236..65532
/gene="Tsl1"
/locus_tag="R103_M10256"
/note="Similar to YML100W 'Large subunit of trehalose
6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex,
which converts uridine-5'-diphosphoglucose and glucose
6-phosphate to trehalose, similar to Tps3p and may share
function; mutant has aneuploidy tolerance'"
/codon_start=1
/product="Tsl1p"
/protein_id="EWG94258.1"
/translation="MALIVASLFLPYQPQFELDTSLPENSQVDSSLVNIQAMANDQQQ
QRALSNNISQESLVAPAPEQGVPPAISRSATRSPSAFNRASSTTNTATLDDLVSSDIF
MENLTANATTSHTPTSKTMLKPRKNGSVERFFSPSSNIPTDRIASPIQHEHDSGSRIA
SPIQQQQQDPTANLLKNVNKSLLVHSLLNNTSQTSLEGPNNHIVTPKSRAGNRPTSAA
TSLVNRTKQGSASSGSSGSSAPPSIKRITPHLTASAAKQRPLLAKQPSNLKYSELADI
SSSETSSQHNESDPDDLTTAPDEEYVSDLEMDDAKQDYKVPKFGGYSNKSKLKKYALL
RSSQELFSRLPWSIVPSIKGNGAMKNAINTAVLENIIPHRHVKWVGTVGIPTDEIPEN
ILANISDSLKDKYDSYPVLTDDVTFKAAYKNYCKQILWPTLHYQIPDNPNSKAFEDHS
WKFYRNLNQRFADAIVKIYKKGDTIWIHDYHLMLVPQMVRDVLPFAKIGFTLHVSFPS
SEVFRCLAQREKILEGLTGADFVGFQTREYARHFLQTSNRLLMADVVHDEELKYNGRV
VSVRFTPVGIDAFDLQSQLKDGSVMQWRQLIRERWQGKKLIVCRDQFDRIRGIHKKLL
AYEKFLVENPEYVEKSTLIQICIGSSKDVELERQIMIVVDRINSLSTNISISQPVVFL
HQDLDFSQYLALSSEADLFVVSSLREGMNLTCHEFIVCSEDKNAPLLLSEFTGSASLL
NDGAIIINPWDTKNFSQAILKGLEMPFDKRRPQWKKLMKDIINNDSTNWIKTSLQDIH
ISWQFNQEGSKIFKLNTKTLMEDYQSSKKRMFVFNIAEPPSSRMISILNDMTSKGNIV
YIMNSFPKPILENLYSRVQNIGLIAENGAYVSLNGVWYNIVDQVDWRNDVAKILEDKV
ERLPGSYYKINESMIKFHTENAEDQDRVASVIGDAITHINTVFDHRGIHAYVYKNVVS
VQQVGLSLSAAQFLFRFYNSASDPLDTSSGQITNIQTPSQQNPSDQEQQPPASPTVSM
NHIDFACVSGSSSPVLEPLFKLVNDEASEGQVKAGHAIVYGDATSTYAKEHVNGLNEL
FTIISRIIED"
gene 65841..66170
/locus_tag="R103_M10261"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(66010..68652)
/gene="Arg81"
/locus_tag="R103_M10266"
mRNA complement(66010..68652)
/gene="Arg81"
/locus_tag="R103_M10266"
/product="Arg81p"
CDS complement(66010..68652)
/gene="Arg81"
/locus_tag="R103_M10266"
/note="Similar to YML099C 'Zinc-finger transcription
factor of the Zn(2)-Cys(6) binuclear cluster domain type,
involved in the regulation of arginine-responsive genes;
acts with Arg80p and Arg82p'"
/codon_start=1
/product="Arg81p"
/protein_id="EWG94259.1"
/translation="MGISSKNGPKKMGRAKTFTGCWTCRGRKVKCDLRHPHCQRCEKS
NLPCGGYDIKLRWSKPMQFDPYGVPIPQNSPATTTNLSGSVDEPQYQRRNIDFVRYGE
EYVYHEDMDDELTMLHTPPIEKISDNKTWIIKKFGVFKGTDKIDKQYAPRKKRNRKRV
AKSLESSASISLSSSPSSSTISFPIRHIEDKLRNKGHVKTGILSANDGVPPTPNLLDY
DWNNLNITGYEWISSELRDDALLSAVTLQGHHLGHTQPQEISLEENSNVVSGEEQVNA
KEHGCAVETDNQGSSTLPNKAASANDKLYQQNLKLLFQKNSSNSEEPDPQALIDDVFV
NIEPRSLPASDLNKITLAPPNEESRMPKSMLELTSYSSDLPPELVDIIPKTDLTVHGL
ARFLLNHYFNNVADKMTVVVLEKNPWKTLYFPRALMALGDLAGLGQSSNSRNALLNAL
LAVSCFHLQSKYPRNYKLQKYFLGLGIELRNQASNFLRLCLNTKSSIPEKYKDVLTAI
LSMNSIDVVWGTMADCQDHLALCEDFVESRMKLRPNISEKAKTLHRIFSFLKLIQDST
ALDKVRAKEIVILPSEEDDNYKLLDTSNATSSSSEPRVDVVQEGLFREALNENDGKIH
IEFVKEPITNVSADSTPSSTTPPIFTNIATESYYNKSDISNLVSKTDENIIGTDSLYG
LPNSLILLFSDCVRIVRHNEYYNLTYLPVPRKFNELSLNFEKRLLKWKSEWNFHQENS
EGKSFINSTAEALYHHTMSFYFSLIIYYFTMARSLNCQFLQNYVAKVLDHLNAMEELV
DQKKVKIVPLIWQGFMAGCACTDENRQQEFRRWAAKLAESGVGSYWGARQVMLEVWRR
RKEDEPGDNWYSIYKDWEMNLMLS"
gene 68880..69383
/gene="Taf13"
/locus_tag="R103_M10271"
mRNA 68880..69383
/gene="Taf13"
/locus_tag="R103_M10271"
/product="Taf13p"
CDS 68880..69383
/gene="Taf13"
/locus_tag="R103_M10271"
/note="Similar to YML098W TFIID subunit (19 kDa), involved
in RNA polymerase II transcription initiation, similar to
histone H4 with atypical histone fold motif of Spt3-like
transcription factors"
/codon_start=1
/product="Taf13p"
/protein_id="EWG94260.1"
/translation="MSRKLKKTNLFNKDVSSLLYAHGDVPQPLQATVQCLDELVSGYL
VDVCTNAFHTAQNSQRNKLRLEDFKFALRKDPIKLGRAEELIATNKLITEAKKQFNET
DNQNSLKRYREEDEEGDEMEEDEDEQQVTDDDEEGVGRNSAKQSTDSKATKIRKQAPK
NLKKTKK"
gene complement(69946..71301)
/gene="Vps9"
/locus_tag="R103_M10286"
mRNA complement(69946..71301)
/gene="Vps9"
/locus_tag="R103_M10286"
/product="Vps9p"
CDS complement(69946..71301)
/gene="Vps9"
/locus_tag="R103_M10286"
/note="Similar to YML097C 'A guanine nucleotide exchange
factor involved in vesicle-mediated vacuolar protein
transport; specifically stimulates the intrinsic guanine
nucleotide exchange activity of Vps21p/Rab5: similar to
mammalian ras inhibitors; binds ubiquitin'"
/codon_start=1
/product="Vps9p"
/protein_id="EWG94261.1"
/translation="MTDDEKREILKEFDPFSQLEQANGNPDKDVKFKKDDPNKAAAEE
TNRDISAQDKGDEEPFYDFQIFIKQLQTPGADPLVKYTKSFLRNFLAQRLLWTVSEEI
KLISDFKTFIYDKFTLYEPFRSLDNSKMRNAKEGMEKLIMGKLYSRCFSPSLYEILQK
PLDDEHMKDLTNDDTLLEKIRHYRFISPIMLDIPDTMPNARLNKFVHLASKELGKINR
FKSPRDKMVCVLNASKVIFGLLKHTKLEQNGADSFIPVLIYCILKGQVRYLVSNVNYI
ERFRSPDFIRGEEEYYLSSLQAALNFIMSLTERSLTIDDHEDFEEAYQRNLKQLAEEK
EEEEKKKQLEVPDELQPNGTLLKPLDEVTNIVISKFNELFSPIGEPTQEETLKSEQSN
KEEDVSSLIKKIEENERKDTLNTLQNMFPDMDPSLIEDVCIAKKSRIGPCVDALLSLS
E"
gene 71521..73098
/locus_tag="R103_M10291"
mRNA 71521..73098
/locus_tag="R103_M10291"
/product="hypothetical protein"
CDS 71521..73098
/locus_tag="R103_M10291"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94262.1"
/translation="MCGILLHYCPNNNHLNDELIEFPEGTEFGDTTCTNESSIFNKII
PYIAARGPNYSSLRAVKAYRISWFSSVLSLRQPFTKQSINVDDRYFLQFNGELYNKEI
SQGDNDSLYIASMVQNLKEGMGVIDVIKSLEGEYAYTIYDVNSSKLYFGRDPIGRRSL
SYSVTPDNELYVASVTGSAGSFQDCIGGVIYEYDTRTKLLNSNQRSHLPYEVTSEIDL
NFTSLSEVSKNLYAVLRDSVKKRVESIHPRHIENSPIAVLFSGGIDCSVIVALICEVL
QENDYKCGKPVIELLNVSFENPRTGLFPSDTPDRKLSINSAKTLQNLYPNIDIKLVEV
DVPYDEYLKWKPFVINLMYPKQTEMDLSIAIAFFFASRGRGFLTSLNGERTPYQRHGI
VLFSGLGADELYGGYHKFANKPPHELVEELTRQINNIYDRNLNRDDKVIAHNGVEVRY
PFLDEYVIKLSTAEIPINFKVNKLILRKVASQYLKLDGISSEPKRAIQFGAKSAKMTK
DGNKHGTDLLKENRNCS"
gene complement(73093..73725)
/gene="Rad10"
/locus_tag="R103_M10301"
mRNA complement(73093..73725)
/gene="Rad10"
/locus_tag="R103_M10301"
/product="Rad10p"
CDS complement(73093..73725)
/gene="Rad10"
/locus_tag="R103_M10301"
/note="Similar to YML095C 'Single-stranded DNA
endonuclease (with Rad1p), cleaves single-stranded DNA
during nucleotide excision repair and double-strand break
repair; subunit of Nucleotide Excision Repair Factor 1
(NEF1); homolog of human ERCC1 protein'"
/codon_start=1
/product="Rad10p"
/protein_id="EWG94263.1"
/translation="MNNTDPTSFESILAGVAKLRKEKSGADTTGSQSLEIDASKLQQQ
EPQTSRRINSNQVINAFNQQKPEEWTDSKATDDYNRKRPFRSTRPGKTVLVNTTQKEN
PLLNHLKSTNWRYVSSTGINMIYYDYLVRGRSVLFLTLTYHKLYVDYISRRMQPLSRN
ENNILIFIVDDNNSEDTLNDITKLCMFNGFTLLLAFNFEQAAKYIEYLNL"
gene complement(73831..74232)
/locus_tag="R103_M10306"
mRNA complement(73831..74232)
/locus_tag="R103_M10306"
/product="hypothetical protein"
CDS complement(73831..74232)
/locus_tag="R103_M10306"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94264.1"
/translation="MSTMNFLLSTILPGMYNDADAGTSSFCPSFPAWETVLISSIHSV
NLPLAMVNACKDCVKCCNSWSNCCFTAFSCSGFSLVKSTKFRTCSNLKVSKVSVRWQQ
RRSYSFTYFFERTWLDFYSALSCLGHSKIFI"
gene 73887..74461
/gene="Gim5"
/locus_tag="R103_M10311"
mRNA join(73887..73902,73986..74461)
/gene="Gim5"
/locus_tag="R103_M10311"
/product="Gim5p"
CDS join(73887..73902,73986..74461)
/gene="Gim5"
/locus_tag="R103_M10311"
/note="Similar to YML094W Subunit of the heterohexameric
cochaperone prefoldin complex which binds specifically to
cytosolic chaperonin and transfers target proteins to it"
/codon_start=1
/product="Gim5p"
/protein_id="EWG94265.1"
/translation="MSSQKIDLTKLNPEQLNAVKQQFDQELQHFTQSLQALTMAKGKF
TECIDDIKTVSQAGNEGQKLLVPASASLYIPGKIVDNKKFMVDIGTGYYVEKSAEAAI
AFYQKKVDKLNKESVQIQDIIKEKTQYSLSIEAQIRQAAIRQHEAMSKQQQQQQKKES
STA"
gene 74702..77401
/gene="Utp14"
/locus_tag="R103_M10316"
mRNA 74702..77401
/gene="Utp14"
/locus_tag="R103_M10316"
/product="Utp14p"
CDS 74702..77401
/gene="Utp14"
/locus_tag="R103_M10316"
/note="Similar to YML093W Subunit of U3-containing Small
Subunit (SSU) processome complex involved in production of
18S rRNA and assembly of small ribosomal subunit"
/codon_start=1
/product="Utp14p"
/protein_id="EWG94266.1"
/translation="MAKKKSKSRSKSSRRVLDALQLAEREINGEFDNSSDNDKRHDAR
RNGTVVNLLKRSKGDTNSDEDDIDSESFEDEELNSDEALGSDDDYDILNSKFSQTIRD
KKENANYKEEEDEGGYTSIDEEDLMPLSQVWDMDEKTAQSNGNDDEDASPQLKLQDTD
ISSESSSSEESESESEDDEEEEDPFDEISEDEEDIELNTITSKLIDETKSKAPKRLDT
YGSGEANEYVLPSANAASGASGKLSLTDMMNVIDDRQVIENANLLKGKSSTYEVPLPQ
RIQQRHDRKAAYEISKQEVSKWNDIVQQNRRADHLIFPLNKPTEHNHSSAFTRTQDVP
QTELQEKVDQVLQESNLANPEKDSKFEELSTAKMTPEEMRKRTTEMRLMRELMFREER
KARRLKKIKSKTYRKIKKKELMKNRELAAVSSDEDNEDHDIARAKERMTLKHKTNSKW
AKDMIKHGMTNDAETREEMEEMLRQGERLKAKMLDRNSDDEEDGRVQTLSDVENEEKE
NIDSEALKSKLGKTGVMNMAFMKNGEAREREANKETLRQLRAVENGDDIKLFESDEEE
TNGENIQINKGRRIYTPGSLESNKDMNELNDHTRKEHKVDESRSLENRLRAKNSGQSK
NARTNAEGAIIVEEESDGEPLQDGQNNQQDEEAKDVNPWLANESDEEHTVKKQSSKVN
VIDKDSSKNVKAMNKMEKAELKQKKKKKGKSNDDEDLLLTADDSTRLKIVDPYGGSDN
EQGDNVFMFKQQDVIAEAFAGDDVVAEFQEEKKRVIDDEDDKEVDTTLPGWGEWAGAG
SKPKNKKRKFIKKVKGVVNKDKRRDKNLQNVIINEKVNKKNLKYQSSAVPFPFENREQ
YERSLRMPIGQEWTSRASHQELIKPRIMTKPGQVIDPLKAPFK"
gene complement(77598..78350)
/gene="Pre8"
/locus_tag="R103_M10321"
mRNA complement(77598..78350)
/gene="Pre8"
/locus_tag="R103_M10321"
/product="Pre8p"
CDS complement(77598..78350)
/gene="Pre8"
/locus_tag="R103_M10321"
/note="Similar to YML092C Alpha 2 subunit of the 20S
proteasome"
/codon_start=1
/product="Pre8p"
/protein_id="EWG94267.1"
/translation="MTDRYSFSLTTFSPSGKLGQIDYALTAVKQGVTSLGIKATNGVV
IATEKKSSSPLAMSETLSKVSLLTPDIGAVYSGMGPDYRVLVDKSRKVAHTSYKRIYG
EYPPTKLLVSEVAKIMQEATQSGGVRPFGVSLLIAGHDEFNGFSLYQVDPSGSYFPWK
ATAIGKGSVAAKTFLEKRWNDELELEDAIHIALLTLKESVEGEFNGDTIELAIIGDEN
PDLLGYTGIPTDKGPRFRKLTSQEINDRLEAL"
gene complement(78672..82280)
/gene="Rpm2"
/locus_tag="R103_M10326"
mRNA complement(78672..82280)
/gene="Rpm2"
/locus_tag="R103_M10326"
/product="Rpm2p"
CDS complement(78672..82280)
/gene="Rpm2"
/locus_tag="R103_M10326"
/note="Similar to YML091C 'Protein subunit of
mitochondrial RNase P, has roles in nuclear transcription,
cytoplasmic and mitochondrial RNA processing, and
mitochondrial translation; distributed to mitochondria,
cytoplasmic processing bodies, and the nucleus'"
/codon_start=1
/product="Rpm2p"
/protein_id="EWG94268.1"
/translation="MAFKSFIYSKGYHRSAAQKKTATSFFDSSYQYLRQNQGLVNSDP
VLHASHLHPHPVVVANVNYNNVDDILHPHDLDSSINNTNNPLTHEELLYNQNVSLRSL
KQQQSINYVNNNNNNQHRYYSTGPTLPTNQYDPLNFSNRNFQDLSLKTSQPSVQQPQN
EYSLLKDENAPVWKEDTEPCLNKSTYLQTHIDEINRCYEQKNYNKINSLYQSLKRNDI
VPPLEIFTKVLDSLCKRPLDNNDLDNKMYELLTCYQDMINNRLKPPDEIYNIVLLSLF
KGSILAYQFKNPNGNDFYKIAIELFNTTTNDPRQKSVVKFRNFSKDVLDYNLLAMNIY
PGHITLSKAQQVIKSSPAFIKDSFYFIACFSYAKLTNDKFAIKELYEDFRLSLSSGSP
DQGLFDDQFEIYSVILSSFIETGEVELATNLLDDLVSKIQSSNGLASNISLLLSSFLI
SMSKVDPSNAYEIWFKFHKLDWIPEFSYEFYLVFMANSFQDWNLTKKIYDYIFPMERN
LSPLKKQKLSDYLLHPIGVDSITTSLLDYSLQLKDNEVIMKILEESIVKTFSFDIGIY
PFVFNYLREIQCGEDYLMRFIESHAEFIKKSNSINKFQFLNMIVDNFQSQSLLNKISH
AKFFKNFVEDFNLENCEMVSYNGLISCINNFIKIPKTIKDFPYILEIHAILVTKLFDF
DTYPILQNGNNEVLLKFRDQIEHQFKMLAQNFCRLNLDPNLLAGVVSQAMKMVNLDDT
ANGQDLLNFFNHPGDWDKSYPLSLGSFIRNSPRGGIREFTKLSKEGYCFDYDTYKELI
IKRAINKQIIDKCLEVCPDSIELKNIVNLMISKIPGRNLTQLIINNPKFSKVFVPNLR
NDSMLKLIENCESLSNFIRVCDFPEKFKSIAIQAENKNAIELIYERLFDGGKYADILR
YNNIVPVLNLELLLKSCIRSGEFKKYENLSKKFNDKISESSKIDIQLEYLINKNDLKG
AFTLFEKTPRELRTPHKTMDLYTFALFLDSFNRNITYYESPENTLQFANILSSQTSFI
NLLSTYNLIAHSDHLMNFNIGGMAAKVKKEILNQMLNNLYDSIRLLSPSIENDKSMKE
KLREKVRNYCRFKACLKSPELDMDELKTLVSVESFLNPFTPSMLFNNLIETIYINEHA
SSLVLQNGLIYSLQQKGLNKILSYLEESFITSGNDANIEKVREFRSLLRKSKPLQA"
gene 83809..85815
/gene="Ufo1"
/locus_tag="R103_M10346"
mRNA 83809..85815
/gene="Ufo1"
/locus_tag="R103_M10346"
/product="Ufo1p"
CDS 83809..85815
/gene="Ufo1"
/locus_tag="R103_M10346"
/note="Similar to YML088W 'F-box receptor protein, subunit
of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase
complex; binds to phosphorylated Ho endonuclease, allowing
its ubiquitylation by SCF and subsequent degradation'"
/codon_start=1
/product="Ufo1p"
/protein_id="EWG94269.1"
/translation="MERPGLVLQDLPPEILINIFSHLDEKDLFTLQELSTHFRNLIHD
EELWKNLFKSRVHTTHFPTFSQSSKFSVEYIERTRGLHHWQHNKAIRTKYTIIPTRNW
DQPSIERIVFDYPRVAAYNDGTITILQLQNHKRQKKFKKLIYIPCTTPQGCSTMDFNI
NAAVFGRFDGRVFGKLLSNKSYLTPVMEFTGRHSAGVTAICNSESWDTSREDWSVSGS
ENGEIIWWCENKLVKMWKVSNRVIWKLAFFKDWTLIMDDEKLYIIHQMQELHSIDIPK
DLDEQPMRVRFFKMDFGSMTLVLADLNNVYTISVNPNGNFGNLRKLEMPEQICAVEID
EKTSQREQNWQFAGDDGCYISLLTTQNTLYIINIRDLSSSGLKVQCKISFDEQVYVSQ
VTNLIVVVALPNVLQILNAMTGELIKTVLKTEKFPEFLKVSQDKIIMGSGNVLNYLKF
VSSDSKKHHHSTKGKNTVSNKWNETLNTELQYYDEDEDLRRKRQSEISRLIDAYGGDL
ELSGDTDEENDIQLRIALLESQEAQARNQAEAGEPVGDDEDEQLRRALEESQLIYETQ
TNSSANHGNSTNDEIDEDDEEFLRAIRQSRVEDERRRHLRNHTTGRRNGPLSDDNFAT
YGAAESSERTSTENTIGSSVGVDASNNVDEDLQLAIACHCVVIE"
assembly_gap 85893..85907
/estimated_length=15
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(85959..86951)
/gene="Aim33"
/locus_tag="R103_M10351"
mRNA complement(85959..86951)
/gene="Aim33"
/locus_tag="R103_M10351"
/product="Aim33p"
CDS complement(85959..86951)
/gene="Aim33"
/locus_tag="R103_M10351"
/note="Similar to YML087C 'Putative protein of unknown
function, highly conserved across species and orthologous
to human CYB5R4; null mutant displays reduced frequency of
mitochondrial genome loss'"
/codon_start=1
/product="Aim33p"
/protein_id="EWG94270.1"
/translation="MSIVETCISFVSTNPFYPFCTGLLLNCVVTPLYFWKTQNGRIVV
VSLLQFVVLYATAFISIGTDKSLYRNKWVALPLSKKTRISRNTSLYCFKLKYPFERLH
IPMGYHLAVRVTINGERLVRYYTPVNVPNTEGHLELVVKTYKHGVVSKYFDKLKIGQY
VEFKGPLGELEYDQDTATELGIIAGGSGITPVLQVLQEIIPSPEDLTHISLIYANETE
DDILMKSQLDHMAKEYPHFKVHYVIHKPNGKWNGDVGYVTLEEMKRYLPKQAEDHRLL
ICGPPKMNEMVLNYAKELGWSNGFHKGNGTDKVFVFNQLFTIDDFSCKYALIIF"
gene complement(87374..88954)
/gene="Alo1"
/locus_tag="R103_M10356"
mRNA complement(87374..88954)
/gene="Alo1"
/locus_tag="R103_M10356"
/product="Alo1p"
CDS complement(87374..88954)
/gene="Alo1"
/locus_tag="R103_M10356"
/note="Similar to YML086C D-Arabinono-1,4-lactone oxidase,
catalyzes the final step in biosynthesis of
dehydro-D-arabinono-1,4-lactone, which is protective
against oxidative stress"
/codon_start=1
/product="Alo1p"
/protein_id="EWG94271.1"
/translation="MSTIPFRKNYVFKNWAGIYSAKPERYFQPSSIDEVVELVKSARL
AEKSLVTVGSGHSPSNMCVTDEWLVNLDRLDKVQKFVEYPELHYADVTVDAGMRLYQL
NEFLGAKGYSIQNLGSISEQSVAGIISTGSHGSSPYHGLISSQYVNLTIVNGKGELKF
LDAENDPEVFKAALLSVGKIGIIVSATIRVVPGFNIKSTQEVITFENLLKQWDTLWTS
SEFIRVWWYPYTRKCVLWRGNKTTDAQNGPAKSWWGTKLGRFFYETLLWISTKIYAPL
TPFVEKFVFNRQYGKLEKSSTGDVNVTDSISGFNMDCLFSQFVDEWGCPMDNGLEVLR
SLDHSIAQAAINKEFYVHVPMEVRCSNTTLPSEPLDTSKRTNTSPGPVYGNVCRPFLD
NTPSHCRFAPLENVTNSQLTLYINATIYRPFGCNTPIHKWFTLFENTMMVAGGKPHWA
KNFLGSTTLAAGPVKKDTDYDDFEMRGMALKVEEWYGEDLKKFRKIRKEQDPDNVFLA
NKQWAIINGIIDPSELSD"
gene complement(89525..90993)
/gene="Tub1"
/locus_tag="R103_M10366"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(91381..92637)
/locus_tag="R103_M10376"
mRNA complement(91381..92637)
/locus_tag="R103_M10376"
/product="hypothetical protein"
CDS complement(91381..92637)
/locus_tag="R103_M10376"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94272.1"
/translation="MDYLHVYNNNQAHVPRTPPGRQCLSPVLPPIILALDQQHSLTFG
YSSPRCLPTPILPSMSTNTPVHWSQQVVTIPVAVRDHAGMMPIITPMTSPRPQFEATA
PSPPLAPVAIKLPDLKLPPSPVPSTVEDRVSHPILPKIVIGNGGGRDVERDSTEELIR
KIPNYIDCAKTKAQLSKVRSGKQLIACAQEYHHPVNKDEIENINNILNFRDFIFKHPK
SSFEFLCTLSFEQFVRVYSFISFIYRTKKINKNKYELVCEMNVHEQLSNKRIQRTRTP
EKYKIHLICESKLILTFNHCTKTVKFESINGGHCHPISANHIIKPSLFLTHCINKCHQ
TVSDPTDLKLALRDALEALDHERIGLSFLKRRHFKCSHQASSLSNASATLKKQEDHNT
FGVHTGIIHTANFFMFNASSDIFQRN"
gene 93449..95398
/locus_tag="R103_M10391"
mRNA 93449..95398
/locus_tag="R103_M10391"
/product="hypothetical protein"
CDS 93449..95398
/locus_tag="R103_M10391"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94273.1"
/translation="MVSAQVATELGQPIPLDTQHAVSVCFPTWKSVISYVEKDPKVLG
CLKSGYPRFWIHPSIQKLRDILIEKYAKENETCFCFPSYRVAKRCREYVRRKCAHRNG
KVRILQLATAKPINEEQKTWKRECKIAVVFVDGAYENILKQYWQYTGEIISSRLAEYV
LHELFMVEKKSSPAEEKEYIEMRYGRNLNFAFADRAKELIKKRIATKVIDKDEHDEEE
NYHFLAGNQDEQDFQDTFLDSSLNEANHGEDHDGGISGEVDSQEEPHNGLVSTIPPEP
IEMSTIEEEQSVEEDAGRCALRVCPERDVFLFPSGMASIFTAHRLLLQWDSLRLNRSR
NGSDVTSSPPNKKTVIFGFPYADTLHVLQEFNETYFLGEGDESSMKELTKILHSGEQI
LAVFIETPSNPLLKMGNLLELKRLSELFGFFIIIDETVGGIVNIDGLPFADIVCSSLT
KTFNGDSNVIGGSMVLNPQSRVYEFASRFMQLEDEYEDLLWCEDAIYLERNSRDFIAR
TIRINYSTEYLLDKILKPHVGENKLFKKIYYPNLTSKETLTNYDMVRCKKEGGYGGLF
SLTFHDEDHAAAFYDNLKLNKGPSLGTNFTLAFPYTLMTYYHELDMAEKFGVERNLLR
ISVGLESQSILGKIFQEAIDKTVEI"
gene complement(95570..95749)
/gene="Atp18"
/locus_tag="R103_M10396"
mRNA complement(95570..95749)
/gene="Atp18"
/locus_tag="R103_M10396"
/product="Atp18p"
CDS complement(95570..95749)
/gene="Atp18"
/locus_tag="R103_M10396"
/note="Similar to YML081C-A no_gene_description"
/codon_start=1
/product="Atp18p"
/protein_id="EWG94274.1"
/translation="MLKRFPTPILKVYWPFFVAGAAVYYGMSKAADLSSNTKEFINDP
RNPRFAKGGKFVEVD"
gene 96364..100119
/locus_tag="R103_M10401"
mRNA 96364..100119
/locus_tag="R103_M10401"
/product="hypothetical protein"
CDS 96364..100119
/locus_tag="R103_M10401"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94275.1"
/translation="MSSEEFKGLPIKRDISSTIYADRPPALSAPPCVGATGNDKIQVL
PIPKKSRTIKTDKPRPFLCHICTRGFVRQEHLKRHQRAHTNEKPFLCVFCGRCFARRD
LVLRHQHKLHSALVSKESINSKDKTEIDAINDKNIIQIQGNKQTILPTPSNPLAKTAA
QLKKAAKEKKNGKQGKLDLSPSYRANNHSTDVSPSVGNSSTPAVIEETDSSSHFPLPD
TNIPTKSKRHASFSASSAFTYSSDNFQKLHQQAKSDFDELQESIPHQVGFSTPQLTAQ
QLIENAIESGVVDLETLDLPPFLSLDGLPPASSSTAVAASEQIDICPSSATDTISGAN
STPNQAATAPPFQLPIARESSSLFLANTPYLSDFLTMGSSYGGSGGFAKSITADPSLD
YFNYKNHSHPDSRHNNSSSGINYSNNKNNNESIEKSQNNSNVINETIDHTDIHAHHAD
AHDDSFIESEEWLSKFIMDSQIDNDLKLNINHFNDIGFNNLHPQNPTTHSEPRNMHNE
NRDMHRSASKFQSVSENISPREQMSLFKTKQNKAISKFLSDEKIPSTASPSSSASPVQ
FDKKNVDINEFLLDESVSNLFTTRQIDLFKKNVNLYSPLFQNQKGAVSSTSLTPSLTT
QTATTQSGPGWTDSSQKLTFFTEQLRNLIIKENNLKSNLFPTVDELNHYVDLYQVEFH
KYFPFIHLYSIIPSSENYPLVISISMIGALYGFHSTHALLLSKIARTRVRMFLENTRS
NHDKTPIWLMQSLVLLTFTSIFSNDMNAFRTVNTQIMILVQLIKISKLNYPLENFIKP
PIESDHVLEYQDNPAVLNQFKAQYNTREQINRNFKYFILAQSRIRICHIVLLISNLFK
SLVDFDCCFHSIDLKCGVPCYNEVLFFCENSRTWNENLTRFNIVLDSKFSLIEVSNGE
SNYEKCLMYLSNGNPYLYKNAKISFKTLLSLLISIHEKINIERDALKDSDESDFHAKN
VQWRMHSRPLVATMLKHWELLYIKNGGILALSDENLPIINTNPSFRLIIPLYFFAKLR
KCLDIAPTLRCIWNQDWNSMNSSLEKVCYEWESLREATEYAVSVITFWIDTVSVMKGK
STQTPIFTITCIFVSILVIAGYMRRLEDFAQNKNSDCMIGSLKSTDRILWLKAFKTLK
RIESHLSEREYKLQTFAEFLRVPDNGSLDIESLDSSLIENTLNSHDVTNQALDIITRT
RLSSRTLYCGARILGDTPVWPVSLLFAHALQSRAIYNINHRKSVNSV"
gene 100392..101663
/gene="Dus1"
/locus_tag="R103_M10406"
mRNA 100392..101663
/gene="Dus1"
/locus_tag="R103_M10406"
/product="Dus1p"
CDS 100392..101663
/gene="Dus1"
/locus_tag="R103_M10406"
/note="Similar to YML080W 'Dihydrouridine synthase, member
of a widespread family of conserved proteins including
Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17'"
/codon_start=1
/product="Dus1p"
/protein_id="EWG94276.1"
/translation="MTEPALSSANNALMQKLTGRQLFDKIGRPTRIVAPMVDQSELAW
RILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDGSSVDRPLVVQFCANDPEYLL
AAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLIHNLINTLHKNLKVPVTAK
IRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLANWETIKYLRDNLPKET
VFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVDQTKNKEKIFPRVDKII
REYFRIVKECQESKASKTAMKSHFFKILRPFLPHHTDIRSTLATMNAKATWEEWEEQV
VKPVEKVVQEIFEQPDIDIKDEITIGEKQSWGGSYRTVPYWRCQPYFRPVNGITGDKR
VMQGLIDESVNKKRKADVPLESADKKKDVKA"
gene 101833..102438
/locus_tag="R103_M10411"
mRNA 101833..102438
/locus_tag="R103_M10411"
/product="hypothetical protein"
CDS 101833..102438
/locus_tag="R103_M10411"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94277.1"
/translation="MSANVQEAANAAIEPASFVKVPMPEPPSSLQQLINDWQLIKHRE
GGYFKETDRSPYTMEVEKPVNGGSGNTEMVTRNQSTLIYYLLTPDSPIGKFHKNINRI
IHILQRGKGQYVLVYPDGQVKSFKVGFDYKNGEVSQWVVPGGVFKASFLLPNEEFDNG
FLISEVVVPGFDFEDHTFLKGEDELKHLVGPEKAAELAFLA"
gene 102599..103147
/gene="Cpr3"
/locus_tag="R103_M10421"
mRNA 102599..103147
/gene="Cpr3"
/locus_tag="R103_M10421"
/product="Cpr3p"
CDS 102599..103147
/gene="Cpr3"
/locus_tag="R103_M10421"
/note="Similar to YML078W 'Mitochondrial peptidyl-prolyl
cis-trans isomerase (cyclophilin), catalyzes the cis-trans
isomerization of peptide bonds N-terminal to proline
residues; involved in protein refolding after import into
mitochondria'"
/codon_start=1
/product="Cpr3p"
/protein_id="EWG94278.1"
/translation="MFKRSIIQQSRLFSNSASRLGKKVFFDPAVNGTKIGRIEFELYD
NVVPKTAENFRALCTGEKGWGYKGVPFHRIIPDFMIQGGDTDLTNGFGGKSIYGSKFA
DENFVKKHDKAGLLSMANAGPNTNGSQFFITTVPCPWLDGKHVVFGEVTKGMDIVKAI
ESYGTASGKPRAEIVIEEAGEL"
gene 103463..103942
/gene="Bet5"
/locus_tag="R103_M10426"
mRNA 103463..103942
/gene="Bet5"
/locus_tag="R103_M10426"
/product="Bet5p"
CDS 103463..103942
/gene="Bet5"
/locus_tag="R103_M10426"
/note="Similar to YML077W Component of the TRAPP
(transport protein particle) complex, which plays an
essential role in the vesicular transport from endoplasmic
reticulum to Golgi"
/codon_start=1
/product="Bet5p"
/protein_id="EWG94279.1"
/translation="MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLL
YGMIFSLRSITQKLSKGSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQV
LQYIYSHIYVKYVSNNLLSPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ"
gene complement(104111..106945)
/gene="War1"
/locus_tag="R103_M10431"
mRNA complement(104111..106945)
/gene="War1"
/locus_tag="R103_M10431"
/product="War1p"
CDS complement(104111..106945)
/gene="War1"
/locus_tag="R103_M10431"
/note="Similar to YML076C 'Homodimeric Zn2Cys6 zinc finger
transcription factor; binds to a weak acid response
element to induce transcription of PDR12 and FUN34,
encoding an acid transporter and a putative ammonia
transporter, respectively'"
/codon_start=1
/product="War1p"
/protein_id="EWG94280.1"
/translation="MDTQIAITGVAVGKEINNNNSKTDQKVSLPKADVPCIDKATQTI
IEGCSKDDPRLSYPTKLETTEKGKTKRNSFACVCCHSLKQKCEPSDVNDIYRKPCRRC
LKHKKLCKFDLSKRTRKRKPRSRSPTSFESPMVNVSTKSKGPTDSEESSLKDGTSYLA
SFPSDPNAKQFPNSRTVLPGLQQSLSDLWSTLSQPPSYGAREAETTSTGEITTNNHTK
SNGSVPTNPAVLASNDEHTNISDAPVIYSTYNSPVPISSAPTSINSEALFKHRPKIVG
DEETQNVKVKRQKKSYSRHMTRSFRKQLQSLIISQKGKIRDISMKLDTWSKQWNDLVE
KSMFLPTIADPVSVGIISHEEATLRLHLYKTEISYLSKLPFIKVEENVSVDELRKKKP
ILFSVIMSCVSIVLTPKQTTRGTIMKLDSFVLNLITNQIFKANNKSIEIIESLSTLCL
WYNFFEWSSKTRYHIFNYICCCLTRDLGPTYVNRSFGMFSDEDPKRFKSPLELYSNGA
SLTLLVYISALNISIFLRQSIQARWSHVTEKACEDLVKETKKSRHYDNDKLLLDSADD
PILVQFAKMNHVLENIHTHLHERDLNDDEFDDPIFTKKYLNKLMEKYHKQLQEIFTKL
DRNRPRVIAFYYSVEAYLYQYKLAVFIGEMSHTINEKVELPREIMDDFVKCYHCCKSA
LEEFSKLEPILITSLPLFHTSRIIYTVGMLLLKLRYSVVAIPSFHDLMPLTDDAIALV
IGVNNLLEKTSELYPFNNSLYKFRYVIALFCQTYANKVIDVADRYNAEREKLKEKQVI
DEVSNGHDGTKPINAYVTESQKMPTEEDPIIDNNTNQNITAVPDEMLPVYSRVRDDTA
AMNLNINSTSYMNESPHEHRESMTGTTLLPPPFISNGVTNSADSTNIKPSPSSSVDNL
NDYLTDINSLAWGVNSLNDEFWTDLFMNDI"
gene complement(107332..110496)
/gene="Hmg1"
/locus_tag="R103_M10441"
mRNA complement(107332..110496)
/gene="Hmg1"
/locus_tag="R103_M10441"
/product="Hmg1p"
CDS complement(107332..110496)
/gene="Hmg1"
/locus_tag="R103_M10441"
/note="Similar to YML075C 'One of two isozymes of HMG-CoA
reductase that catalyzes the conversion of HMG-CoA to
mevalonate, which is a rate-limiting step in sterol
biosynthesis; localizes to the nuclear envelope;
overproduction induces the formation of karmellae'"
/codon_start=1
/product="Hmg1p"
/protein_id="EWG94281.1"
/translation="MPLLFKGLKQMAKPIAYVSRFSAKRPIHIILFSLIISAFAYLSV
IQYYFNGWQLDSNSVFETAPNKDSNTLFQECSHYYRDSSLDGWVSITAHEASELPAPH
HYYLLNLNFNSPNETDSIPELANTVFEKDNTKYILQEDLSVSKEISSTDGTKWRLRSD
RKSLFDVKTLAYSLYDVFSENVTQADPFDVLIMVTAYLMMFYTIFGLFNDMRKTGSNF
WLSASTVVNSASSLFLALYVTQCILGKEVSALTLFEGLPFIVVVVGFKHKIKIAQYAL
EKFERVGLSKRITTDEIVFESVSEEGGRLIQDHLLCIFAFIGCSMYAHQLKTLTNFCI
LSAFILIFELILTPTFYSAILALRLEMNVIHRSTIIKQTLEEDGVVPSTARIISKAEK
KSVSSFLNLSVVVIIMKLSVILLFVFINFYNFGANWVNDAFNSLYFDKERVSLPDFIT
SNASENFKEQAIVSVTPLLYYKPIKSYQRIEDMVLLLLRNVSVAIRDRFVSKLVLSAL
VCSAVINVYLLNAARIHTSYTADQLVKTEVTKKSFTAPVQKASTPVLTNKTVISGSKV
KSLSSAQSSSSGPSSSSEEDDSRDIESLDKKIRPLEELEALLSSGNTKQLKNKEVAAL
VIHGKLPLYALEKKLGDTTRAVAVRRKALSILAEAPVLASDRLPYKNYDYDRVFGACC
ENVIGYMPLPVGVIGPLVIDGTSYHIPMATTEGCLVASAMRGCKAINAGGGATTVLTK
DGMTRGPVVRFPTLKRSGACKIWLDSEEGQNAIKKAFNSTSRFARLQHIQTCLAGDLL
FMRFRTTTGDAMGMNMISKGVEYSLKQMVEEYGWEDMEVVSVSGNYCTDKKPAAINWI
EGRGKSVVAAATIPGDVVRKVLKSDVSALVELNIAKNLVGSAMAGSVGGFNAHAANLV
TAVFLALGQDPAQNVESSNCITLMKEVDGDLRISVSMPSIEVGTIGGGTVLEPQGAML
DLLGVRGPHATAPGTNARQLARIVACAVLAGELSLCAALAAGHLVQSHMTHNRKPAEP
TKPNNLDATDINRLKDGSVTCIKS"
gene complement(111687..112913)
/gene="Fpr3"
/locus_tag="R103_M10451"
mRNA complement(111687..112913)
/gene="Fpr3"
/locus_tag="R103_M10451"
/product="Fpr3p"
CDS complement(111687..112913)
/gene="Fpr3"
/locus_tag="R103_M10451"
/note="Similar to YML074C 'Nucleolar peptidyl-prolyl
cis-trans isomerase (PPIase); FK506 binding protein;
phosphorylated by casein kinase II
(Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p'"
/codon_start=1
/product="Fpr3p"
/protein_id="EWG94282.1"
/translation="MSDLLPLATYSLNVEPYTPVPAIDVTMPITVRITMAALNPEAID
EENKPSTLRIIKRNPDFEDDDFLGGDFDEDEIDEESSEEEEEEKTQKKKKSKGKKAES
ESEDDEEDDDEDDEFQESVLLTLSPEAQYQQSLDLTITPEEEVQFIVTGSYAISLSGN
YVKHPFDTPMGVEGEDEDEDADIYDSEDYDLTPDEDEIIGDDMDDLDDEEEEEVRIEE
VQEEDEEDNDGEEEQEEEEQKEEVKPEPKKSKKEKKRKHEEKEEEKKAKKVKKVEFKK
DLEEGPTKPKSKKEQDKHKPKSKVLEGGIVIEDRTIGNGPQAKRGARVGMRYIGKLKN
GKVFDKNTSGKPFAFKLGRGEVIKGWDIGVAGMSVGGERRIIIPAPYAYGKQALPGIP
ANSELTFDVKLVSMKN"
gene complement(114816..115762)
/gene="Rpl6a"
/locus_tag="R103_M10466"
mRNA complement(join(114816..115331,115748..115762))
/gene="Rpl6a"
/locus_tag="R103_M10466"
/product="Rpl6ap"
CDS complement(join(114816..115331,115748..115762))
/gene="Rpl6a"
/locus_tag="R103_M10466"
/note="Similar to YML073C 'N-terminally acetylated protein
component of the large (60S) ribosomal subunit, has
similarity to Rpl6Bp and to rat L6 ribosomal protein;
binds to 5.8S rRNA'"
/codon_start=1
/product="Rpl6ap"
/protein_id="EWG94283.1"
/translation="MSAQKAPKWYPSEDVAALKKTRKAARPQKLRASLVPGTVLILLA
GRFRGKRVVYLKHLEDNTLLISGPFKVNGVPLRRVNARYVIATSTKVSVEGVNVEKFN
VEYFAKEKLTKKEKKEANLFPEQQNKEIKAERVEDQKVVDKALIAEIKKTPLLKQYLS
ASFSLKNGDKPHMLKF"
gene complement(116321..120957)
/gene="Tcb3"
/locus_tag="R103_M10471"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(121340..123163)
/gene="Cog8"
/locus_tag="R103_M10486"
mRNA complement(121340..123163)
/gene="Cog8"
/locus_tag="R103_M10486"
/product="Cog8p"
CDS complement(121340..123163)
/gene="Cog8"
/locus_tag="R103_M10486"
/note="Similar to YML071C Component of the conserved
oligomeric Golgi complex (Cog1p through Cog8p), a
cytosolic tethering complex that functions in protein
trafficking to mediate fusion of transport vesicles to
Golgi compartments"
/codon_start=1
/product="Cog8p"
/protein_id="EWG94284.1"
/translation="MELILNSLISDDLTEEQKRLSLDFLQDILQSNTKDYESYFSSRA
VPGSITEDIAEIDAELSALDRKIRKSLLDNKSQIIGNILENDDRAQLDDIAKSLEQLW
ELDTNINKAADRNVTNDDINNESVSIDDFLEDDKEDNDTGRIMTTESNNLARKRKEDE
FHKALSRLRNRISTKEDDKDDIRSDTLVTVLENLDSITDLMELPFLARTCIRTGHYQE
AVMLYTHTTSLRSRFPGSTIVDEVCEKVLNEISTTMLSGLVKLLSTNVSVNSLKKILQ
YLNSIPPFDGKTNKSLLSVFLAMRYKFITDEIASYPLDVESSNESLIEMMVKRKIEVL
REHVYMSLNVFLKSFLYDTNDLEIPFPEELESTVLRINGTNEEKEIEEKEKETKKEEY
QKQDSVANNEEDLTENKSIEDVQEEVQGKVEGEDDGAERKTENEIENETVNKTEDKAE
KGKEEEVNTKDNKAEKEEEEINKVEVTPEEPSKSIDNKAEKEEEEINKVEVTPEEPSK
KIRTSKRENKIPTNAVMLQFVDKCITYVLKDLTRGLNSIKLSDSVCLQLVYCSFRLCD
LNRNYHHLFLKKINDTSLFTTEQLARAIDKRAELASKYIYS"
gene complement(123416..123487)
/locus_tag="R103_M10496"
tRNA complement(123416..123487)
/locus_tag="R103_M10496"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg3);
TR(UCU)M2"
gene 125046..126800
/gene="Dak1"
/locus_tag="R103_M10511"
mRNA 125046..126800
/gene="Dak1"
/locus_tag="R103_M10511"
/product="Dak1p"
CDS 125046..126800
/gene="Dak1"
/locus_tag="R103_M10511"
/note="Similar to YML070W 'Dihydroxyacetone kinase,
required for detoxification of dihydroxyacetone (DHA);
involved in stress adaptation'"
/codon_start=1
/product="Dak1p"
/protein_id="EWG94285.1"
/translation="MSAKSFEVTDPVNSSLKGFALANPSITLVPEEKILFRKTDSDKI
ALISGGGSGHEPTHAGFIGKGMLSGAVVGEIFASPSTKQILNAIRLVNENASGVLLIV
KNYTGDVLHFGLSAERARALGINCRVAVIGDDVAVGREKGGMVGRRALAGTVLVHKIV
GAFAEEYSSKYGLDGTAKVAKIINDNLVTIGSSLDHCKVPGRKFESELNEKQMELGMG
IHNEPGVKVLDPIPSTEDLISKYMLPKLLDPNDKDRAFVKFDEDDEVVLLVNNLGGVS
NFVISSITSKTTDFLKENYNITPVQTIAGTLMTSFNGNGFSITLLNATKATKALQSDF
EEIKSVLDLLNAFTNAPGWPIADFEKTSAPSVNDDLLHNEVTAKAVGTYDFDKFAEWM
KSGAEQVIKSEPHITELDNQVGDGDCGYTLVAGVKGITENLDKLSKDSLSQAVAQISD
FIEGSMGGTSGGLYSILLSGFSHGLIQVCKSKDEPVTKEIVAKSLGIALDTLYKYTKA
RKGSSTMIDALEPFVKEFTASKDFNKAVKAAEEGAKSTATFEAKFGRASYVGDSSQVE
DPGAVGLCEFLKGVQSAL"
gene 127073..128731
/gene="Pob3"
/locus_tag="R103_M10516"
mRNA 127073..128731
/gene="Pob3"
/locus_tag="R103_M10516"
/product="Pob3p"
CDS 127073..128731
/gene="Pob3"
/locus_tag="R103_M10516"
/note="Similar to YML069W Subunit of the heterodimeric
FACT complex (Spt16p-Pob3p), which associates with
chromatin via interaction with Nhp6Ap and Nhp6Bp, and
reorganizes nucleosomes to facilitate access to DNA by RNA
and DNA polymerases"
/codon_start=1
/product="Pob3p"
/protein_id="EWG94286.1"
/translation="MSTDFDRIYLNQSKFSGRFRIADSGLGWKISTSGGSAANQARKP
FLLPATELSTVQWSRGCRGYDLKINTKNQGVIQLDGFSQDDYNLIKNDFHRRFNIQVE
QREHSLRGWNWGKTDLARNEMVFALNGKPTFEIPYARINNTNLTSKNEVGIEFNIQDE
EYQPAGDELVEMRFYIPGVIQTNVDENMTKKEESSNEVVPKKEDGAEGEDVQMAVEEK
SMAEAFYEELKEKADIGEVAGDAIVSFQDVFFTTPRGRYDIDIYKNSIRLRGKTYEYK
LQHRQIQRIVSLPKADDIHHLLVLAIEPPLRQGQTTYPFLVLQFQKDEETEVQLNLED
EDYEENYKDKLKKQYDAKTHIVLSHVLKGLTDRRVIVPGEYKSKYDQCAVSCSFKANE
GYLYPLDNAFFFLTKPTLYIPFSDVSMVNISRAGQTSTSSRTFDLEVVLRSNRGSTTF
ANISKEEQQLLEQFLKSKNLRVKNEDREVQERLQTALGSDSDEEDINMGSAGEDDESV
DEDFQVSSDNDADEVAEEFDSDAALSDAEGGSDEERPSKKPKVE"
gene 129113..130507
/gene="Itt1"
/locus_tag="R103_M10521"
mRNA 129113..130507
/gene="Itt1"
/locus_tag="R103_M10521"
/product="Itt1p"
CDS 129113..130507
/gene="Itt1"
/locus_tag="R103_M10521"
/note="Similar to YML068W Protein that modulates the
efficiency of translation termination, interacts with
translation release factors eRF1 (Sup45p) and eRF3
(Sup35p) in vitro, contains a zinc finger domain
characteristic of the TRIAD class of proteins"
/codon_start=1
/product="Itt1p"
/protein_id="EWG94287.1"
/translation="MALTQFENDLEILRDMYPELEMKSVKVEEEGEFPQRINGKLLFK
ISLLADVNIEFDEQHMLLSNLSNECVEFTIYSCHYPDIRRCVVMDIKSLWISTDEKKM
LIDKALRLVEETVDMSIEFADSFTSILILIFGFLIDDTAILLFPNGIRKCLTQDQYDL
FKQIGEEATLQKMSRSNYHCCICMEMEKGVRMIKLPCENANVEHYLCRGCAKSYFTAM
IQENRISSVRCPQCEYKELKLEDFKSYKKMLKALFTPLIPVSFLKEVIDTELCERYEK
MFYNQAATRLSKYCPYACVTCRRCDSWCTKEDLDDAMIQCQKCHFVFCFDCLHAWHGY
NNKCGKKVSLSTDIIEEYLDDTVTSYERKRKLEAKYGRRVLELEVNDYLAEKMLDLAI
KKEGSNLQRCPKCKVVVERSEGCNKMKCEVCGMLFCFICGVLLYPEDPYEHFREAYSG
CYGRLFEGMPGTET"
gene complement(130626..131779)
/gene="Erv41"
/locus_tag="R103_M10526"
mRNA complement(join(130626..131653,131749..131779))
/gene="Erv41"
/locus_tag="R103_M10526"
/product="Erv41p"
CDS complement(join(130626..131653,131749..131779))
/gene="Erv41"
/locus_tag="R103_M10526"
/note="Similar to YML067C 'Protein localized to
COPII-coated vesicles, forms a complex with Erv46p;
involved in the membrane fusion stage of transport; has
homology to human ERGIC2 (PTX1) protein'"
/codon_start=1
/product="Erv41p"
/protein_id="EWG94288.1"
/translation="MAGLKTFDAFPKTEEQYKKKSTKGGLTSLLTYLFLLFIAWTEFG
EYFGGYIDQQYVVDSQVRDTVQINMDIYVNTKCDWLQINVRDQTMDRKLVLEELQLEE
MPFFIPYDTKVNDINEIITPELDEILGEAIPAEFREKLDTRSFFDESDPNRAHLPEFN
GCHIFGSIPVNRVSGELQITAKSLGYVASRKAPLEELKFNHVINEFSFGDFYPYIDNP
LDNTAQFNQDEPLTTYVYYTSVVPTLFKKLGAEVDTNQYSVNDYRYLYKDVAAKGDKM
PGIFFKYNFEPLSIVVSDVRLSFIQFLVRLVAICSFLVYCASWIFTLLDMALITIMGP
KWSLRYQPDDKTKGILDR"
gene complement(131989..133098)
/gene="Sma2"
/locus_tag="R103_M10536"
mRNA complement(131989..133098)
/gene="Sma2"
/locus_tag="R103_M10536"
/product="Sma2p"
CDS complement(131989..133098)
/gene="Sma2"
/locus_tag="R103_M10536"
/note="Similar to YML066C 'Meiosis-specific prospore
membrane protein; required to produce bending force
necessary for proper assembly of the prospore membrane
during sporulation'"
/codon_start=1
/product="Sma2p"
/protein_id="EWG94289.1"
/translation="MLFPKRLIVWGVLLILSLSQFVLYLPATTCTNSKGLRLCAPQFT
ITVIGGSSTANEFIASVREFLRLISYLTIDMGWSNEFTDPSVYEDENLVDTFQPDKVF
ELNYFGFCKRSNKSKVYCTSNENYGMDVLEVLVRDVGIQLGNISTTRSNETKKFGDSL
VLTYRLALTSIRDFLKHDKHTGNALSKALIGSPDPNVKGASPTKNYLKGVNLAFILMM
FNGMVFYFAVLEIIVGFLSICVVSAFGGALSVGKRHRLFPILLKSSSSILVVIATLTI
LCNIVYLIALKTLEPEEVTDVGSDNAAVHTTGWELLKVNVGSGFIMGLARYAIQWVLL
VLAFLAANHYKAKPKKSDKYTEDTSNSPSPDLMEK"
gene 133773..136517
/gene="Orc1"
/locus_tag="R103_M10541"
mRNA 133773..136517
/gene="Orc1"
/locus_tag="R103_M10541"
/product="Orc1p"
CDS 133773..136517
/gene="Orc1"
/locus_tag="R103_M10541"
/note="Similar to YML065W 'Largest subunit of the origin
recognition complex, which directs DNA replication by
binding to replication origins and is also involved in
transcriptional silencing; exhibits ATPase activity'"
/codon_start=1
/product="Orc1p"
/protein_id="EWG94290.1"
/translation="MAKTLKDLQGWEIITTDEQGNIIDGGQKRLRRRGAKTEHYLKRS
SDGIKLGRGDSVVMHNEAAGTYSVYMIQELRLNTLNNVVELWALTYLRWFEVNPLAHY
RQFNPDANILNRPLNYYNKLFSETANKNELYLTAELAELQLFNFIRVANVMDGSKWEV
LKGNVDPERDFTVRYICEPTGEKFVDINIEDVKAYIKKVEPREAQEYLKDLTLPSKKK
EIKRGPQKKDKATQTAQISDAETRATDITDNEDGNEDESSNYESPSDIDVSEDMDSGE
ISADELEEDEDEEEDEDEEEKEARHTNSPRKRGRKIKLGKDDIDASVQPPPKKRGRKP
KDPSKPRQMLLISSCRANNTPVIRKFTKKNVARAKKKYTPFSKRFKSIAAIPDLTSLP
EFYGNSSELMASRFENKLKTTQKHQIVETIFSKVKKQLNSSYVKEEILKSANFQDYLP
ARENEFASIYLSAYSAIESDSATTIYVAGTPGVGKTLTVREVVKELLSSSAQREIPDF
LYVEINGLKMVKPTDCYETLWNKVSGERLTWAASMESLEFYFKRVPKNKKKTIVVLLD
ELDAMVTKSQDIMYNFFNWTTYENAKLIVIAVANTMDLPERQLGNKITSRIGFTRIMF
TGYTHEELKNIIDLRLKGLNDSFFYVDTKTGNAILIDAAGNDTTVKQTLPEDVRKVRL
RMSADAIEIASRKVASVSGDARRALKVCKRAAEIAEKHYMAKHGYGYDGKTVIEDENE
EQIYDDEDKDLIESNKAKDDNDDDDDNDGVQTVHITHVMKALNETLNSHVITFMTRLS
FTAKLFIYALLNLMKKNGSQEQELGDIVDEIKLLIEVNGSNKFVMEIAKTLFQQGSDN
ISEQLRIISWDFVLNQLLDAGILFKQTMKNDRICCVKLNISVEEAKRAMNEDETLRNL
"
gene complement(136702..137439)
/gene="Tem1"
/locus_tag="R103_M10546"
mRNA complement(136702..137439)
/gene="Tem1"
/locus_tag="R103_M10546"
/product="Tem1p"
CDS complement(136702..137439)
/gene="Tem1"
/locus_tag="R103_M10546"
/note="Similar to YML064C 'GTP-binding protein of the ras
superfamily involved in termination of M-phase; controls
actomyosin and septin dynamics during cytokinesis'"
/codon_start=1
/product="Tem1p"
/protein_id="EWG94291.1"
/translation="MATPSTGANNSIPAVRNQVEVQVGLVGDAQVGKTSLMVKYVQNI
YDKEYTQTLGVNFLKRKVSIRSTDIIFSIMDLGGQREFINMLPIATVGSSVIIFLFDL
TRPETLSSIKEWYRQAYGLNDSAIPILVGTKYDLLIDLDPEYQEQISRTSMKYAQVMN
APLIFCSTAKSINIQKIFKIALAKIFNLTLTIPEINEIGDPLLIYKHLGGQQHRHHNK
SQDRKSHNIRKPSSSPSSKAPSPGVNT"
gene 138038..138805
/gene="Rps1b"
/locus_tag="R103_M10551"
mRNA 138038..138805
/gene="Rps1b"
/locus_tag="R103_M10551"
/product="Rps1bp"
CDS 138038..138805
/gene="Rps1b"
/locus_tag="R103_M10551"
/note="Similar to YML063W 'Ribosomal protein 10 (rp10) of
the small (40S) subunit; nearly identical to Rps1Ap and
has similarity to rat S3a ribosomal protein'"
/codon_start=1
/product="Rps1bp"
/protein_id="EWG94292.1"
/translation="MAVGKNKRLSRGKKGLKKKVVDPFTRKEWFDIKAPSTFENRNVG
KTLVNKSTGLKNASDALKGRVVEVCLADLQGSEDHSFRKVKLRVDEVQGKNLLTNFHG
MDFTTDKLRSMVRKWQTLIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSH
IRAIRKVISEILTREVQNSTLAQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLL
KQPKFDVGALMALHGEGSGEEKGKKVSGFKDEVLETV"
gene complement(139061..140239)
/gene="Mft1"
/locus_tag="R103_M10556"
mRNA complement(139061..140239)
/gene="Mft1"
/locus_tag="R103_M10556"
/product="Mft1p"
CDS complement(139061..140239)
/gene="Mft1"
/locus_tag="R103_M10556"
/note="Similar to YML062C 'Subunit of the THO complex,
which is a nuclear complex comprised of Hpr1p, Mft1p,
Rlr1p, and Thp2p, that is involved in transcription
elongation and mitotic recombination; involved in telomere
maintenance'"
/codon_start=1
/product="Mft1p"
/protein_id="EWG94293.1"
/translation="MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIING
TLSNDDSKIEKLTEQNISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLEN
LKDKLPDIKSIHSKLLLRIGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELG
TDLVKFLIEKNYLKLVDPGLKKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQ
EVSSYKEKWLRDAEIFGKITSIFKEELLKRDGLLNEAEGDNIDEDYESDEDEERKERF
KRQRSMVEVNTIENVDEKEESDHEYDDQEDEENEEEDDMEVDVEDIKEDNEVDGESSQ
QEDNSRQGNNEETDKETGVIEEPDAVNDAEEADSDHSSRKLGGTTSDFSASSSVEEVK
"
gene complement(140509..143088)
/gene="Pif1"
/locus_tag="R103_M10566"
mRNA complement(140509..143088)
/gene="Pif1"
/locus_tag="R103_M10566"
/product="Pif1p"
CDS complement(140509..143088)
/gene="Pif1"
/locus_tag="R103_M10566"
/note="Similar to YML061C 'DNA helicase; exists in a
nuclear form that acts as a catalytic inhibitor of
telomerase; and as a mitochondrial form involved in repair
and recombination of mitochondrial DNA; mutations affect
zinc and iron homeostasis'"
/codon_start=1
/product="Pif1p"
/protein_id="EWG94294.1"
/translation="MPKWIRSTLNHIIPRRPFICSFNSFLLLKNVSHAKLSFSMSSRG
FRSNNFIQAQLKHPSILSKEDLDLLSDSDDWEEPDCIQLETEKQEKKIITDIHKEGPV
DKKPMRDKNVMNFINKDSPLSWNDMFKPSIIQPPQLISENSFDQSSQKKSRSTGFKNP
LRPALKKESSFDELQNNSISQERSLEMINENEKKKMQFGEKIAVLTQRPSFTELQNDQ
DDSNLNPHNGVKVKIPICLSKEQESIIKLAENGHNIFYTGSAGTGKSILLREMIKVLK
GIYGRENVAVTASTGLAACNIGGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWEN
IGALVVDEISMLDAELLDKLDFIARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRP
TKFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGNIDDETEREFKKLSRP
LPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALEDEELKERLLQNFL
APKELHLKVGAQVMMVKNLDATLVNGSLGKVIEFMDPETYFCYEALTNDPSMPPEKLE
TWAENPSKLKAAMEREQSDGEESAVASRKSSVKEGFAKSDIGEPVSPLDSSVFDFMKR
VKTDDEVVLENIKRKEQLMQTIHQNSAGKRRLPLVRFKASDMSTRMVLVEPEDWAIED
ENEKPLVSRVQLPLMLAWSLSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREG
LQVLNFDRTRIKAHQKVIDFYLTLSSAESAYKQLEADEQVKKRKLDYAPGPKYKAKSK
SKSNSPAPISATTQSNNGIAAMLQRHSRKRFQLKKESNSNQVHSLVSDEPRGQDTEDH
ILE"
gene 143427..144557
/gene="Ogg1"
/locus_tag="R103_M10571"
mRNA 143427..144557
/gene="Ogg1"
/locus_tag="R103_M10571"
/product="Ogg1p"
CDS 143427..144557
/gene="Ogg1"
/locus_tag="R103_M10571"
/note="Similar to YML060W Mitochondrial glycosylase/lyase
that specifically excises 7,8-dihydro-8-oxoguanine
residues located opposite cytosine or thymine residues in
DNA, repairs oxidative damage to mitochondrial DNA,
contributes to UVA resistance"
/codon_start=1
/product="Ogg1p"
/protein_id="EWG94295.1"
/translation="MSYKFGKLAINKSELCLANVLQAGQSFRWIWDEKLNQYSTTMKI
GQQEKYSVVILRQDEENEILEFVAVGDCGNQDALKTHLMKYFRLDVSLKHLFDNVWIP
SDKAFAKLSPQGIRILAQEPWETLISFICSSNNNISRITRMCNSLCSNFGNLITTIDG
VAYHSFPTSEELTSRATEAKLRELGFGYRAKYIIETARKLVNDKAEANITSDTTYLQS
ICKDAQYEDVREHLMSYNGVGPKVADCVCLMGLHMDGIVPVDVHVSRIAKRDYQISAN
KNHLKELRTKYNALPISRKKINLELDHIRLMLFKKWGSYAGWAQGVLFSKEIGGTSGS
TTTGTIKKRKWDMIKETEAIVTKQMKLKVELSDLHIKEAKID"
gene 147623..149485
/gene="Mub1"
/locus_tag="R103_M10576"
mRNA 147623..149485
/gene="Mub1"
/locus_tag="R103_M10576"
/product="Mub1p"
CDS 147623..149485
/gene="Mub1"
/locus_tag="R103_M10576"
/note="Similar to YMR100W 'MYND domain-containing protein
required for ubiquitination and turnover of Rpn4p;
interacts with Ubr2p (E3) and indirectly with Rad6p (E2);
short-lived protein degraded in a Ubr2p/Rad6p dependent
manner; similar to the A. nidulans samB gene'"
/codon_start=1
/product="Mub1p"
/protein_id="EWG94296.1"
/translation="MRDSNHRSLTSNKPIVTITSTVYDRRALDINSSIPLINSLNYLT
YLTSNSSKVRETVANDGALERLVSILRSCHLSLFELLDLDLENFNEHENIKDLWKEKR
LALCAWKWTLTFQCLVLTGTRGTEQIRKKVVMSGVLSVLVTVLDNYLLYHKNYDFIKD
QTMTFDFKGITTETMYKFMRKDENETYQQYIEFITGQDKLKLSTDKNFLNERLVAPSM
TIPTDFSDIWGRFADLASNFEPDQERHDDDIDIDSEVESENFDAHKNFFSQPDINRPT
ISTPREFFLGRIVPKQDDVIWSLQLLAFVSKYTYMKSTLQNVELVESLSFRSMAYKIK
QRISEENDLEEQERDVTVKLSSLYPYLSKNPENNSKVKALDTSKMDPFFKELEELSNR
CQQEEQNEICNNHCPVLNLFERYRVPKPSDDNAYGKDKERINLRKKISDNFERRWSYD
KMKKELTNIVYKNKVLTNVVNIFPLVEKYTVSAENTHDVIYWSSVIMRNSCRKNEILG
VRQCANFSCGKWEDFPRQFAKCRRCKRTKYCSRKCQLKAWGYHRYWCHEVGSSHMRST
NTTTGVNTPNEPSSLNATATTAADVSNSTSTFTPNISTTVPDEISNRDENSIPE"
assembly_gap 149565..149575
/estimated_length=11
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 149945..150151
/gene="Hug1"
/locus_tag="R103_M10581"
mRNA 149945..150151
/gene="Hug1"
/locus_tag="R103_M10581"
/product="Hug1p"
CDS 149945..150151
/gene="Hug1"
/locus_tag="R103_M10581"
/note="Similar to YML058W-A no_gene_description"
/codon_start=1
/product="Hug1p"
/protein_id="EWG94297.1"
/translation="MTMDQGLNPKQFFLDDVVLQDTLCSMSNRVNKSVKTGYLFPKDH
VPSANIIAVERRGGLSDIGKNTSN"
gene 150568..150882
/gene="Sml1"
/locus_tag="R103_M10591"
mRNA 150568..150882
/gene="Sml1"
/locus_tag="R103_M10591"
/product="Sml1p"
CDS 150568..150882
/gene="Sml1"
/locus_tag="R103_M10591"
/note="Similar to YML058W 'Ribonucleotide reductase
inhibitor involved in regulating dNTP production;
regulated by Mec1p and Rad53p during DNA damage and S
phase'"
/codon_start=1
/product="Sml1p"
/protein_id="EWG94298.1"
/translation="MQNSQDYFYAQNRCQQQQAPSTLRTVTMAEFRRVPLPPMAEVPM
LSTQNSMGSSASASASSLEMWEKDLEERLNSIDHDMNNNKFGSGELKSMFNQGKVEEM
DF"
gene complement(151222..151599)
/locus_tag="R103_M10596"
mRNA complement(151222..151599)
/locus_tag="R103_M10596"
/product="hypothetical protein"
CDS complement(151222..151599)
/locus_tag="R103_M10596"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94299.1"
/translation="MAGTLFIILRFVDTTLPSSRVYCVRSLEVSVAVELAAATVLAFE
SIGVVDDCGRSVLFSIILIAAFICSVFLIASEDIAGSRRSTGSCVTLWEGRNISFCLY
RSNWLNTVPVGYMFFLRKNRSLD"
gene 151367..153181
/gene="Cmp2"
/locus_tag="R103_M10601"
mRNA 151367..153181
/gene="Cmp2"
/locus_tag="R103_M10601"
/product="Cmp2p"
CDS 151367..153181
/gene="Cmp2"
/locus_tag="R103_M10601"
/note="Similar to YML057W 'Calcineurin A; one isoform (the
other is CNA1) of the catalytic subunit of calcineurin, a
Ca++/calmodulin-regulated protein phosphatase which
regulates Crz1p (a stress-response transcription factor),
the other calcineurin subunit is CNB1'"
/codon_start=1
/product="Cmp2p"
/protein_id="EWG94300.1"
/translation="MSSDAIRNTEQINAAIKIIENKTERPQSSTTPIDSKASTVAAAN
STATETSRDLTQYTLDDGRVVSTNRRIMNKVPAITSHVPTDEELFQPNGIPRHEFLRD
HFKREGKLSAAQAARIVTLATELFSKEPNLISVPAPITVCGDIHGQYFDLLKLFEVGG
DPATTSYLFLGDYVDRGSFSFECLIYLYSLKLNFNDHFWLLRGNHECKHLTSYFTFKN
EMLHKYNLDIYEKCCESFNNLPLAALMNGQYLCVHGGISPELNSLQDINNLNRFREIP
SHGLMCDLLWADPIEEYDEVLDKDLTEEDIVNSKTMVPHHGKMAPSRDMFVPNSVRGC
SYAFTYRAACHFLQETGLLSIIRAHEAQDAGYRMYKNTKTLGFPSLLTLFSAPNYLDT
YNNKAAILKYENNVMNIRQFNMTPHPYWLPDFMDVFTWSLPFVGEKVTEMLVAILNIC
TEDELENDTPVIEELVGTDKKLPQAGKSEATPQPATSASPKHASILDDEHRRKALRNK
ILAVAKVSRMYSVLREETNKVQFLKDHNSGVLPRGALSNGVKGLDEALSTFERARKHD
LINEKLPPSLDELKKENKKYYEKVWQKVHEHDAKNDSK"
gene complement(153381..155363)
/gene="Imd4"
/locus_tag="R103_M10606"
mRNA complement(join(153381..154495,154904..155363))
/gene="Imd4"
/locus_tag="R103_M10606"
/product="Imd4p"
CDS complement(join(153381..154495,154904..155363))
/gene="Imd4"
/locus_tag="R103_M10606"
/note="Similar to YML056C Inosine monophosphate
dehydrogenase, catalyzes the first step of GMP
biosynthesis, member of a four-gene family in S.
cerevisiae, constitutively expressed"
/codon_start=1
/product="Imd4p"
/protein_id="EWG94301.1"
/translation="MSAAPLDYKKALEHLKTYSSKDGLSVQELMDSTTRGGLTYNDFL
VLPGLVNFPSSAVSLQTKLTKKITLNTPFVSSPMDTVTEADMAIYMALLGGIGFIHHN
CTPKEQASMVKKVKMFENGFINSPIVISPTTTVGEVKVMKRKFGFSGFPVTEDGKCPG
KLVGLVTSRDIQFLEDDSLVVSEVMTKNPVTGIKGITLKEGNEILKQTKKGKLLIVDD
NGNLVSMLSRADLMKNQNYPLASKSATTKQLLCGAAIGTIEADKERLRLLVEAGLDVV
ILDSSQGNSVFELNMLKWVKESFPGLEVIAGNVVTREQAANLIAAGADGLRIGMGTGS
ICITQEVMACGRPQGTAVYNVCQFANQFDVPCMADGGVQNIGHITKALALGSSTVMMG
GMLAGTTESPGEYFYKDGKRLKAYRGMGSIDAMQKTGNKGNASTSRYFSESDSVLVAQ
GVSGAVVDKGSIKKFIPYLYNGLQHSCQDIGCESLTLLKENVQNGEVRFEFRTASAQL
EGGVHNLHSYEKRLYN"
gene 155975..156510
/gene="Spc2"
/locus_tag="R103_M10626"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 156604..156623
/estimated_length=20
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(156715..158490)
/gene="Cyb2"
/locus_tag="R103_M10631"
mRNA complement(156715..158490)
/gene="Cyb2"
/locus_tag="R103_M10631"
/product="Cyb2p"
CDS complement(156715..158490)
/gene="Cyb2"
/locus_tag="R103_M10631"
/note="Similar to YML054C 'Cytochrome b2 (L-lactate
cytochrome-c oxidoreductase), component of the
mitochondrial intermembrane space, required for lactate
utilization; expression is repressed by glucose and
anaerobic conditions'"
/codon_start=1
/product="Cyb2p"
/protein_id="EWG94302.1"
/translation="MLKYKPLLKISKNCEAAILRASKTRLNTIRAYGSTVPKSKSFEQ
DSRKRTQSWTALRVGAILAATSSVAYLNWHNGQIDNEPKLDMNKQKISPAEVAKHNKP
DDCWVVINGYVYDLTRFLPNHPGGQDVIKFNAGKDVTAIFEPLHAPNVIDKYIAPEKK
LGPLQGSMPPELVCPPYAPGETKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTK
QAWAYYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSA
TALCKLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLY
VNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNTKAGPKAMKKTNV
EESQGASRALSKFIDPSLTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVL
SNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKG
VGLGRPFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGVTSIAELKPDLLDLSTLKAR
TVGVPNDVLYNEVYEGPTLTEFEDA"
gene complement(158804..158962)
/locus_tag="R103_M10646"
mRNA complement(158804..158962)
/locus_tag="R103_M10646"
/product="hypothetical protein"
CDS complement(158804..158962)
/locus_tag="R103_M10646"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94303.1"
/translation="MIPFPAQHEIFHAYIGRITPHSSRCIANMWHSAHFFHENSLSIM
KTLVPWTL"
gene 159640..159728
/locus_tag="R103_M10651"
tRNA 159640..159728
/locus_tag="R103_M10651"
/product="tRNA-Tyr"
/note="TY(GUA)M1"
assembly_gap 159897..160235
/estimated_length=339
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 161235..162143
/gene="Sur7"
/locus_tag="R103_M10666"
mRNA 161235..162143
/gene="Sur7"
/locus_tag="R103_M10666"
/product="Sur7p"
CDS 161235..162143
/gene="Sur7"
/locus_tag="R103_M10666"
/note="Similar to YML052W 'Plasma membrane protein that
localizes to furrow-like invaginations (MCC patches);
component of eisosomes; associated with endocytosis, along
with Pil1p and Lsp1p; sporulation and plasma membrane
sphingolipid content are altered in mutants'"
/codon_start=1
/product="Sur7p"
/protein_id="EWG94304.1"
/translation="MVKVWNIVLRLVVLLFLAGNTLLLILMIISGATDHYPVNRFYWV
QGNTTGIPNAGDETRWTFWGACLQDKDGSDTCTSNLAPAYPISPVDNFNTHINVPHQF
ISKRDAFYYLTRFSFCFFWIALAFVGVSFILYVLTWCSKMLSEMVLILMSFGFVFNTA
AVVLQTAASAMAKNAFHDDHRSAQLGASMMGMAWASVFLCIVEFILLVFWSVRARLAS
TYSIDNSRYRTSSRWNPFHREKEQATDPILTATGPEDMQQSASIVGPSSNANPVTATA
ATENQPKGINFFTIRKSHERPDDVSV"
gene 162427..163734
/gene="Gal80"
/locus_tag="R103_M10671"
mRNA 162427..163734
/gene="Gal80"
/locus_tag="R103_M10671"
/product="Gal80p"
CDS 162427..163734
/gene="Gal80"
/locus_tag="R103_M10671"
/note="Similar to YML051W 'Transcriptional regulator
involved in the repression of GAL genes in the absence of
galactose; inhibits transcriptional activation by Gal4p;
inhibition relieved by Gal3p or Gal1p binding'"
/codon_start=1
/product="Gal80p"
/protein_id="EWG94305.1"
/translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLS
SQFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEV
VMPLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILR
AKELISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDIL
QYMTSSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVS
CSFKGGKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANG
QQAPLDPGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQ
GFDFEGFPTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
gene 163971..164905
/gene="Aim32"
/locus_tag="R103_M10681"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(165051..169136)
/gene="Rse1"
/locus_tag="R103_M10686"
mRNA complement(165051..169136)
/gene="Rse1"
/locus_tag="R103_M10686"
/product="Rse1p"
CDS complement(165051..169136)
/gene="Rse1"
/locus_tag="R103_M10686"
/note="Similar to YML049C 'Protein involved in pre-mRNA
splicing; component of the pre-spliceosome; associates
with U2 snRNA; involved in ER to Golgi transport'"
/codon_start=1
/product="Rse1p"
/protein_id="EWG94306.1"
/translation="MWGGGKMAVVSLSPHTAKMRKLFGQASTTMAYDGLKREAERRTR
SDHNITMVAKDDELYLYHLTLKKQTNFVHSCIGHFVDLEAGSKREQSQLCVATETHLE
LYDTADGELKLIAKFQNLFATITSMKSLDLPHSGSRAKASNWPTFLALTSDSGNLSIV
QIIMHAGALRLKTLVNQPLTRTTLRRVSPISYMEIDPNGRCIILSSVEQNKLCFLVDY
AQKLRISSPLEIIRPHMVTLDMAVVDVNFNNPCFVTLEIDNAATQLSVHLIFYVLELG
LNHIVKKADYLVNPSANFVLSLPDLSRYNITTSLSDNNYDADYDTLFNPFVVIGFENH
ILVKDMNGFFSLKVEIPKRSITNSRHKNVTIISGIVQKLKNDFFVLLQSNHGDLFKLT
VSPDTNDRNRPLVQLSYFDTIQNSHQLHIFKNGYLFALSEMNNNFLFQFEKLGVEKND
FSNVLTSKDPNKSLVFEPSIKLQNLSILSQQLNLNPSIKSQIVSDSPLSIATKHFTNN
KIITLTNAVNYSNLISTSLPPNATKLWLIPDPATTGDNNTLLFITFPKKTMILQIDNE
SMEELTPDEATRSAFKLSQDTTIHTCLMGSHSIIQVCTAELRHIVPTGKSRYSNKLTW
VPPAGIRIVCATSSKTQLIISLSNYELVYFKIDVSSDSLIELTTHPELDTMPSKVAIV
QDTQHADLLAIADNEGMIKIMSLKDQKEDFLTVISLQLVSEKISDMIMVRDSSIGQLN
LHVGLENGVYMKFHIGDVDGSFTDIKRRFLGLKPVSLSYLREISVSLNNEEEEEEEED
DDDEKEEEEINSSGAKWMSCVVCHSSSTWVSYTWKNVWTIRQLKDQNMLSCSKFVNAD
VAINGVCSISSSGRLNIGRVSNFPTLDNWFHVHESSVNKQENGGGDESNEEEEDEMEE
EMEMLQISTFRPRTILSFPNNPKSILFIDNHSGKKQCRISLQIDGECLKFGSSDHLYK
ILDDIDCVSAAIIDFTRQADHLIICAGDKRLLTYKILVNKDKLSFDIELLHQTEIISP
IHAMLKFKNFLLTAMGSTIVLYGLGKKQLLRRSVTQTPVSITKIVSMHQWNYERLAVG
DIHESVTLFIWDPAGNVFIPFVDDSVKRHVTVLKFLDEATVIGADRYGNAWTLRSPPE
CEKIMSNHDPSELSNGAIKYPLDVITLQQKLPNTYDCKFKFQLLNHFFVNDIITDFHI
LDSLSNSDRPGCIYMGLQGTVGCFIPLLSKGNVFMMGNIENIMAEADDTFYLDYESRK
KNNNMRKEDDEEESGSVVLQGRHGIEDEIICEGSCSILGRDHQEYRSYYAPVRKVIDG
DLCENFLRLSLNEQEFLAKNLKSVQVEDIIQTINEVRTNYM"
gene 169257..170468
/gene="Gsf2"
/locus_tag="R103_M10691"
mRNA 169257..170468
/gene="Gsf2"
/locus_tag="R103_M10691"
/product="Gsf2p"
CDS 169257..170468
/gene="Gsf2"
/locus_tag="R103_M10691"
/note="Similar to YML048W 'ER localized integral membrane
protein that may promote secretion of certain hexose
transporters, including Gal2p; involved in
glucose-dependent repression'"
/codon_start=1
/product="Gsf2p"
/protein_id="EWG94307.1"
/translation="MEIYIRLNADVEHDYAFQVSNEDTINNKIKKIFPSKTGLADLMV
LRPSIFHEKEPVKFYKSIHPGYLSEGGCLMFHYEADNEENLEELNDSKPLIDQLWPGQ
LVVPEWKLSKKNIWVYTIIMLAWLYTDLPDAISPTPGICLTNQLSRLLIPVAKHMDLP
EIAAKLEQEVQANYSSLVAQWLFFVMHIFKVGIITLFLKLGIANPISFNPYKLWSLRD
LTSPSANGAKNSGGNNNTTDLKTRLRSLGWIGAKRATYDDYQTNYYNYVIDKMGGAVA
AYRAGAIRKAAAPGIQLVAGEGFQSPLEDRFTASTFTAIKTERKFILSEEYFVELENN
LKKILEEYDGDIGKMNAEIRRFRRFGIYEPDEKLASLVKLRREIADEKEKASNSDATF
GIKKNDLKKSN"
gene 170730..171096
/locus_tag="R103_M10696"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(170855..171913)
/gene="Prm6"
/locus_tag="R103_M10701"
mRNA complement(170855..171913)
/gene="Prm6"
/locus_tag="R103_M10701"
/product="Prm6p"
CDS complement(170855..171913)
/gene="Prm6"
/locus_tag="R103_M10701"
/note="Similar to YML047C 'Pheromone-regulated protein,
predicted to have 2 transmembrane segments; regulated by
Ste12p during mating'"
/codon_start=1
/product="Prm6p"
/protein_id="EWG94308.1"
/translation="MESSLQKLKFQDIDINLIPTAKWTTKLQYILYTWCQSILHVAMF
FSDIYTCIKLLAFNTWSNNIIQPFLEFRISKWLFSGCILCSSLILIWELVIGLRVYRK
KEITSNYMNGISRLINCLFNFKKYQIFELIVLTDEKKFSKWLFFSYFEISGCLRLLFG
DSPRQIINGLTLWSVLLTVSNETSSGTHSTQSLGNLDDLNGIINKIKHIAKTNYEESV
ILSFMLFSFIIWVILISKLILSIIIFIIFIRPRFLSSKRKVKGYELKLRKYVSKVIDE
NLSRTVYELGILIDDEEEGTICGDNKTQKKFDYDSPDYGDESTIPSYYCYSDVETYER
VYTPIKAYFPQKYKHKYI"
gene 172312..174201
/gene="Prp39"
/locus_tag="R103_M10706"
mRNA 172312..174201
/gene="Prp39"
/locus_tag="R103_M10706"
/product="Prp39p"
CDS 172312..174201
/gene="Prp39"
/locus_tag="R103_M10706"
/note="Similar to YML046W U1 snRNP protein involved in
splicing, contains multiple tetriatricopeptide repeats"
/codon_start=1
/product="Prp39p"
/protein_id="EWG94309.1"
/translation="MPDETNFTIEDIEPRPDALRGLDTQFLHDNTALVQAYRGLDWSD
ISSLTQMVDVIEQTVVKYGNPNDSIKLALETILWQILRKYPLLFGFWKRFATIEYQLF
GLKKSIAVLATSVKWFPTSLELWCDYLNVLCVNNPNETDFIRNNFEIAKDLIGKQFLS
HPFWDKFIEFEVGQKNWHNVQRIYEYIIEVPLHQYARFFTSYKKFLNEKNLKTTRNID
IILRKTQTTVNEIWQFESKIKQPFFNLGQVLNDDLENWSRYLKFVTDPSKSLDKEFVM
SVFDRCLIPCLYHENTWMMYIKWLTKKNISDEVVVDIYQKANTFLPLDFKTLRYDFLR
FLKRKYRSNNTLFNNIFNETVSRYLKIWPNDILLMTEYLCMLKRHSFKNSLDQSPKEI
LEKQTSFTKILETSISNYINNQIDAKVHLQTLINDKNLSIVVVELIKTTWLVLKNNMQ
TRKYFNLYQKNILIKNSVPFWLTYYKFEKSNVNFTKLNKFIRELGVEIYLPTTVMNDI
LTDYKTFYLTHSNIVTYESSIIDSNTFDPILYPELKMSNPKYDPVLNTTANVDWHKKT
EWKEAGHIGITTERPQISNSIIECNSGTLIQKPISLPNFRNLEKINQVKMNDLYTEEF
LKEGK"
gene complement(174735..174805)
/locus_tag="R103_M10711"
tRNA complement(174735..174805)
/locus_tag="R103_M10711"
/product="tRNA-Gly"
/note="TG(GCC)M"
assembly_gap 174841..174853
/estimated_length=13
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(175244..176767)
/gene="Rrn11"
/locus_tag="R103_M10716"
mRNA complement(175244..176767)
/gene="Rrn11"
/locus_tag="R103_M10716"
/product="Rrn11p"
CDS complement(175244..176767)
/gene="Rrn11"
/locus_tag="R103_M10716"
/note="Similar to YML043C 'Component of the core factor
(CF) rDNA transcription factor complex; CF is required for
transcription of 35S rRNA genes by RNA polymerase I and is
composed of Rrn6p, Rrn7p, and Rrn11p'"
/codon_start=1
/product="Rrn11p"
/protein_id="EWG94310.1"
/translation="MFEVPITLTNRKFAQRRKLKYQYINYISRRFDRISKKSTTTDSL
PTPENSAAENNDEEEGQNSEAGAYRRSVLQQKKRRRERHWRSVVGEIYSTTESETDSQ
EEEAEEGGEHDTGIDKEDSDEERKFWKKYEKPEKSFEIWRTVSSQNKQPLNKQKMTYH
NFKKIEKIPLRKMEIPLLHCTKENKLYFQSISRGLEPLKTSTSEVRNYRTRHIVTLTD
LLHLNVSRHNWSLAYKIFATLIRIPGVQIKSLWGIGVEILDNLSNSSSGLDFLQWMCQ
IYSSKSRFVQNINYRSIVPPFQTGSRTHTAKFAITYLWSSLINCQKSMEPSPNIIDKP
FDTENDLLQELIDKISEWVLTPPFMEDAEVWFIYASCHLLKADTLSRQFVNDNKNNDL
IGLDRDIKINQVIKHIHYVRTFLKICLDKGGFAVPSRLIENQLKSFESRLYGEAQDIQ
ERDVANVYDSIDNSSVENSFGDVYETNAEFLDTQLMDLSPEDNGLDEMHYSDEDSSE"
assembly_gap 177002..177021
/estimated_length=20
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 177796..179808
/gene="Cat2"
/locus_tag="R103_M10721"
mRNA 177796..179808
/gene="Cat2"
/locus_tag="R103_M10721"
/product="Cat2p"
CDS 177796..179808
/gene="Cat2"
/locus_tag="R103_M10721"
/note="Similar to YML042W Carnitine acetyl-CoA transferase
present in both mitochondria and peroxisomes, transfers
activated acetyl groups to carnitine to form
acetylcarnitine which can be shuttled across membranes"
/codon_start=1
/product="Cat2p"
/protein_id="EWG94311.1"
/translation="MRICHSRTLSNLKDLPITSRRAMHSAIVNYSTQKAQFPVETNNG
EHYWAEKPNKFYQNKRPNFQGITFAKQQDLPSLPVPELKSTLDKYLQTIRPFCNGVET
FERQQLLCKDFSEHMGPILQDRLKEYANDKRNWMAKFWDEQSYLQYNDPIVPYVSYFY
SHMPLPNHLSKIDNDPLIKATAIISTVVKFIEAIKDESLPVEIIKGMPFCMNSFSLMF
NTSRLPGKPEDNQDTNIFYSVYENNFVTIAYKGKFYKLMTHDGNDKPLSENEIWRQLY
SVVFQGSQSDPKLGGIGSLTSLPRDQWREVHMELMKDPISQDSLETIHKSSFMLCLDL
DQSPVTLEEKSRNCWHGDGINRFYDKSLQFLVTGNGSSGFLAEHSKMDGTPTLFLNNY
VCRQLNKLDVDDFMRKVITPSSTVAMKPMELPFIITPKIHKAIESAQLQFKETIGEHD
LRVWHYNKYGKTFIKRHGMSPDAFIQQVIQLAVFKYLKRQLPTYEAASTRKYFKGRTE
TGRSVSTASLEFVSKWQNGDVPIAEKIQALKHSAKEHSTYLRNAANGNGVDRHFFGLK
NMLKSNDDQIPPLFKDPLFNYSSTWLISTSQLSSEYFDGYGWSQVNDNGFGLAYMLNN
EWLHINIVNKPAKSGASVNRLHYYLSQAADEIFDALENENKRKAKL"
gene complement(179921..180763)
/gene="Vps71"
/locus_tag="R103_M10731"
mRNA complement(179921..180763)
/gene="Vps71"
/locus_tag="R103_M10731"
/product="Vps71p"
CDS complement(179921..180763)
/gene="Vps71"
/locus_tag="R103_M10731"
/note="Similar to YML041C 'Nucleosome-binding component of
the SWR1 complex, which exchanges histone variant H2AZ
(Htz1p) for chromatin-bound histone H2A; required for
vacuolar protein sorting'"
/codon_start=1
/product="Vps71p"
/protein_id="EWG94312.1"
/translation="MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSR
PVKRINYSLADLEARLYTSRSEGDGNSISRQDDRNSKNSLSFEERYTQQEILQSDRRF
MELNTENFSDLPNVPTLLSDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSK
YGHGWVLKPETLREIQLSYKSTKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLN
LMDKNVIYHNVYNKRYFKVLPLITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETR
CRNR"
gene complement(181078..181180)
/locus_tag="R103_M10736"
tRNA complement(181078..181180)
/locus_tag="R103_M10736"
/product="tRNA-Pro"
/note="TP(UGG)M"
gene complement(182005..183333)
/gene="Ymd8"
/locus_tag="R103_M10741"
mRNA complement(182005..183333)
/gene="Ymd8"
/locus_tag="R103_M10741"
/product="Ymd8p"
CDS complement(182005..183333)
/gene="Ymd8"
/locus_tag="R103_M10741"
/note="Similar to YML038C Putative nucleotide sugar
transporter, has similarity to Vrg4p"
/codon_start=1
/product="Ymd8p"
/protein_id="EWG94313.1"
/translation="MNRTVFLAFVFGWYFCSIALSIYNRWMFDPKDGLGIGYPVLVTT
FHQATLWLLSGIYIKLRHKPVKNVLRKNNGFNWSFFLKFLLPTAVASAGDIGLSNVSF
QYVPLTIYTIIKSSSIAFVLLFGCIFKLEKFHWKLALSVIIMFVGVALMVFKPSDSTS
TKNDQALVIFGSFLVLASSCLSGLRWVYTQLMLRNNPIQTNTAAAVEESDGALFTENE
DNVDNEPVVNLANNKMLENFGESKPHPIHTIHQLAPIMGITLLLTSLLVEKPFPGIFS
SSIFRLDTSNGGVGTETTVLSIVRGIVLLILPGFAVFLLTICEFSILEQTPVLTVSIM
GIVKELLTVIFGIIILSERLSGFYNWLGMLIIMADVCYYNYFRYKQDLLQKYHSVSTQ
DNRNELKGFQDFEQLGSKKIAPYSISVDLTNQEYELDMIAQNVSRSSQQV"
gene complement(183615..184637)
/locus_tag="R103_M10746"
mRNA complement(183615..184637)
/locus_tag="R103_M10746"
/product="hypothetical protein"
CDS complement(183615..184637)
/locus_tag="R103_M10746"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94314.1"
/translation="MDENKIIDQLFSKEYTPQDDSEQAKNGDVSLYGLLDEVANGRRL
MNCLFHSPMQMGNKLSTDKLDGKCRQIQRDWIDEEKTITMNSGALQLDGPVLFSWSHN
VAPTSHQETINTTFKQGSPSRGSNKPKITTTSQLFDRASAEIDKCIKPNSKSWMVEER
FERNEAHTADGKKPSTWANSDFKVDPLQKFVVKELPKEKKKSDGDKTKKNKSKRKSFF
GFWGHSGSKSGSKKKSEKPIEAKNEIQDEVSQKSGLSPDDDTTFSDKNTIQSKQESMS
DQQAEPKVHEPAVTNTGCSEHDDGDGFEQVPAQSSYHPSSEPSIASTPSLTLDSFIPL
QPKKKI"
gene 184871..185522
/gene="Cgi121"
/locus_tag="R103_M10751"
mRNA join(184871..185326,185433..185522)
/gene="Cgi121"
/locus_tag="R103_M10751"
/product="Cgi121p"
CDS join(184871..185326,185433..185522)
/gene="Cgi121"
/locus_tag="R103_M10751"
/note="Similar to YML036W 'Component of the EKC/KEOPS
complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS
complex is required for t6A tRNA modification and may have
roles in telomere maintenance and transcription; Cgi121p
is dispensable for tRNA modification'"
/codon_start=1
/product="Cgi121p"
/protein_id="EWG94315.1"
/translation="MVVSIIPQFPDIKVSLALFEQVKNAKEIRSKMSELSTSFAFIDP
RLVCSGEQMYSAIYKTLIEVKYNKMRTRNLNSECVLCLSPTSNISDAFLKFGIKDDSS
QLICLKFHTNTDDVDKEQLRTIMTSIVKGQEIEFNDDNLSRFYDEALIRKIYKLSDDF
KPQDVNGLSRALVDAIQLRGV"
gene complement(185657..188089)
/gene="Amd1"
/locus_tag="R103_M10756"
mRNA complement(185657..188089)
/gene="Amd1"
/locus_tag="R103_M10756"
/product="Amd1p"
CDS complement(185657..188089)
/gene="Amd1"
/locus_tag="R103_M10756"
/note="Similar to YML035C 'AMP deaminase, tetrameric
enzyme that catalyzes the deamination of AMP to form IMP
and ammonia; may be involved in regulation of
intracellular adenine nucleotide pools'"
/codon_start=1
/product="Amd1p"
/protein_id="EWG94316.1"
/translation="MDNQATQRLNDLSLEPAPSHDEQDGSGLVIDIDQRKIGDEQAGV
VVDDETPPLEQQDSHESLAADSRNANFSYHENQQLLENGTKQLALDEHDSHSAILEQP
SHSTNCSSSNITAMNKGHDSADHASQNSGGKPRTLSASAQHILPETLKSFAGAPVVNK
QVRTSASYKMGMLADDASQQFLDDPSSELIDLYSKVAECRNLRAKYQTISVQNDDQNP
KNKPGWVVYPPPPKPSYNSDTKTVVPVTNKPDAEVFDFTKCEIPGEDPDWEFTLNDDD
SYVVHRSGKTDELIAQIPTLRDYYLDLEKMISISSDGPAKSFAYRRLQYLEARWNLYY
LLNEYQETSVSKRNPHRDFYNVRKVDTHVHHSACMNQKHLLRFIKHKLRHSKDEKVIF
RDGKLLTLDEVFRSLHLTGYDLSIDTLDMHAHKDTFHRFDKFNLKYNPIGESRLREIF
LKTNNYIKGTYLADITKQVIFDLENSKYQNCEYRISVYGRSLDEWDKLASWVIDNKVI
SHNVRWLVQIPRLYDIYKKTGIVQSFQDICKNLFQPLFEVTKNPQSHPKLHVFLQRVI
GFDSVDDESKVDRRFHRKYPKPSLWEAPQNPPYSYYLYYLYSNVASLNQWRAKRGFNT
LVLRPHCGEAGDPEHLVSAYLLAHGISHGILLRKVPFVQYLYYLDQVGIAMSPLSNNA
LFLTYDKNPFPRYFKRGLNVSLSTDDPLQFSYTREPLIEEYSVAAQIYKLSNVDMCEL
ARNSVLQSGWEAQIKKHWIGKDFDKSGVEGNDVVRTNVPDIRINYRYDTLSTELELVN
HFANFKRTIEEK"
gene complement(188656..189054)
/locus_tag="R103_M10766"
mRNA complement(188656..189054)
/locus_tag="R103_M10766"
/product="hypothetical protein"
CDS complement(188656..189054)
/locus_tag="R103_M10766"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94317.1"
/translation="MGEADFSLRARLLLRGEAIDGEDEGRSIFTIPSSLGRTFRYIFR
NCGICGLTFSSNSPTSAFFLALDGKSTLFSTNILLKVATFIEFGSKPSSKYSKSLFIA
ALTEYCYRSTVCCSGLYVHSVWSQSTINND"
gene 188753..191383
/gene="Src1"
/locus_tag="R103_M10771"
mRNA join(188753..190672,190799..191383)
/gene="Src1"
/locus_tag="R103_M10771"
/product="Src1p"
CDS join(188753..190672,190799..191383)
/gene="Src1"
/locus_tag="R103_M10771"
/note="Similar to YML034W 'Inner nuclear membrane protein;
functions in regulation of subtelomeric genes and is
linked to TREX (transcription export) factors; SRC1
produces 2 splice variant proteins with different
functions; alternative splicing of SRC1 pre-mRNA is
promoted by Hub1p; mutant has aneuploidy tolerance'"
/codon_start=1
/product="Src1p"
/protein_id="EWG94318.1"
/translation="MNSDLEYLEDGFDPNSMKVATLRRILVENNVDFPSNARKNALVG
LFDEKVKPQIPQLRKMYLNVRPSDEGIVKMDRPSSSPSIASPRRSRRARREKSASPMA
KQFKKNRILDDVSNDDDDDDDDDDDNDKKDDPLIVPSGTDTDEVDDEEDDVITSSSNK
SDTNDFQQNSDTRKKRKDPDSDDWSESNSKENKIDNKHLNLLSSDSEIEQDYQKAKKR
KTSDLNQEHGNGSAILGKLSVKTPIKNTNRKPVSMDNFNDSLTSSGTENDPFVPNIRH
NPKELGTANGTGHSTPLSKLKVSASFADKLPQKEVPSTILVPEVEQQEPSQSERTPSL
FSSEGSGSESEAPLLPEITTPGPHQPMGNTSNNVVEMIDTDSSNLVSDEDEVLVPTRI
ETPQLPTEKDVEKCEARVQELQEEVNEQLEHENGSEFDVKQGSGKVGNRHKFKRALKF
LSKSLLALFLFCIFIVIPLLFGLWYREQRLLIGYCGHEVPSHRVSGNSFEFIQKLDNL
LQDYRPKCIPCPPNGICYPYLKLKCKPDYKLAPSRLDFLEIIPAQGKCVKDDKKQQLV
SEVVEKSLEFLRAKNAQISCGDGKDDIESGMTEDALYQIFNEARAPWIRDDEFEDLWI
QVIKDLTEEPEILWRQLSPTDNNIGGNSNNIIKTNDVPRQKRHLPEKFISKTRNFRST
SKKYIGMKCRFEREIYQTYKKFQRPIWLMFLLIVISKVIEIKLKNYYRKKARIEELVT
QTMEKLKFQKIKSMSDPKENAYLSIVQLRDIFLSDIVDLKYKNQLWSEVVKYLEHNNS
NIKSNLTEIRGEIMKCWEWIGPMELNEPKDSAENKI"
gene complement(191743..193158)
/gene="Rad52"
/locus_tag="R103_M10776"
mRNA complement(191743..193158)
/gene="Rad52"
/locus_tag="R103_M10776"
/product="Rad52p"
CDS complement(191743..193158)
/gene="Rad52"
/locus_tag="R103_M10776"
/note="Similar to YML032C 'Protein that stimulates strand
exchange by facilitating Rad51p binding to single-stranded
DNA; anneals complementary single-stranded DNA; involved
in the repair of double-strand breaks in DNA during
vegetative growth and meiosis'"
/codon_start=1
/product="Rad52p"
/protein_id="EWG94319.1"
/translation="MNETMDMDEKKPVFGNHSEDIQTKLDKKLGPEYISKRVGFGTSR
IAYIEGWRVINLANQIFGYNGWSTEVKSVVIDFLDERQGKFSIGCTAIVRVTLTSGTY
REDIGYGTVENERRKPAAFERAKKSAVTDALKRSLRGFGNALGNCLYDKDFLAKIDKV
KFDPPDFDENNLFRPTDEISESSRTNTLHENQEQQQYPNKRRQLTKVTNTNPDSTKNL
VKIENTVSRGTPMMAAPAEANSKNSSNKDTDLKSLDASKQDQDDLLDDSLMFSDDFQD
DDLINMGNTNSNVLTTEKDPVVAKQSPTASSNPEAEQITFVTAKAATSVQNERYIGEE
SIFDPKYQAQSIRHTVDQTTSKHVPASVLKDKTMTTARDSVYEKFAPKGKQLSMKNND
KELGPHMLEGAGNQVPRETTPIKTNATAFPPAAAPRFAPPSKVVHPNGNGAVPAVPQQ
RSTRREVGRPKINPLHARKPT"
gene complement(193314..193649)
/locus_tag="R103_M10781"
mRNA complement(193314..193649)
/locus_tag="R103_M10781"
/product="hypothetical protein"
CDS complement(193314..193649)
/locus_tag="R103_M10781"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94320.1"
/translation="MIKRETRLRAKILGINANASSHNGECGNASSEITTVWIIEHMKN
NLVTKWLNRVLHTSLKMVWYVYRGFNNSLGVCIICLPCNVLSSSQSHLLIAKESALPV
SDSRCYYIL"
gene 193417..195384
/gene="Ndc1"
/locus_tag="R103_M10786"
mRNA 193417..195384
/gene="Ndc1"
/locus_tag="R103_M10786"
/product="Ndc1p"
CDS 193417..195384
/gene="Ndc1"
/locus_tag="R103_M10786"
/note="Similar to YML031W 'Nuclear envelope protein with
multiple putative transmembrane domains, required for
nuclear pore complex assembly and spindle pole body
duplication; required for meiosis II'"
/codon_start=1
/product="Ndc1p"
/protein_id="EWG94321.1"
/translation="MIQTPRELLNPRYTYHTIFSDVCKTRFNHLVTRLFFICSIIQTV
VISLLALPHSPLWELALAFIPNILALNLVSLLIIVTRKNYMHVKNFGFANSLTFILGQ
LLSVKFLVYQGVYSMGSILLSFVLGVVFGRGGSGWKPYYKLFIWLVVPTIYNLQHHVT
DADKLSFNCENFFQAPQDYVLERVKRIMEKSVILSVISMFVLPIFTTVFFSRQKSGLF
DSFTNGVLAVTNLLIISCIIFITFEFINIAFDAHMSIGCLHKGKLISNLSSTPMETLL
SGLSADKPFTRLTAYQELAYRATSLDPSLRAPIYHSKFRSSSGNTWSLILNECLKTIQ
INNEKVVQYLRSVQDLGGSATARHKKKVENLDYMYENGKLTSANERLFGNRPSMMAPL
RDNGLLDESPNRLRVRTDDSVLLNRGNKKRHRSSYYDNDLDETTQTFNGSIFTHETTF
MTAMRLMLKKLKNSIMSFIFPSYAERQSSDESDNYRLLPNGSNKAQISIIDIWSISKK
RQAEKLVPLPICHANSVVALTGLLIRSKTEDPKGGIIASVGDILKTLERSICALGEFA
DWDPESMAYTAFQTQRTAQDRVQQDSEDEDSMKDTTDMISVLYQLSTSAFMEIVLEYN
VALNDVYLDADVAKLANWFLEVYASGNPNAT"
gene 195663..196142
/gene="Aim31"
/locus_tag="R103_M10791"
mRNA 195663..196142
/gene="Aim31"
/locus_tag="R103_M10791"
/product="Aim31p"
CDS 195663..196142
/gene="Aim31"
/locus_tag="R103_M10791"
/note="Similar to YML030W 'Putative protein of unknown
function; GFP-fusion protein localizes to mitochondria;
may interact with respiratory chain complexes III or IV;
null mutant is viable and displays reduced frequency of
mitochondrial genome loss'"
/codon_start=1
/product="Aim31p"
/protein_id="EWG94322.1"
/translation="MSRMPSSFDVTERDLDDMTFGERIIYHCKKQPLVPIGCLLTTGA
VILAAQNVRLGNKWKAQYYFRWRVGLQAATLVALVAGSFIYGTSGKELKAKEEQLKEK
AKMREKLWIQELERREEETEARRKRAELARMKTLENEEEIKNLEKELSDLENKLGKK"
gene 196590..199105
/gene="Usa1"
/locus_tag="R103_M10796"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 199365..199955
/gene="Tsa1"
/locus_tag="R103_M10806"
mRNA 199365..199955
/gene="Tsa1"
/locus_tag="R103_M10806"
/product="Tsa1p"
CDS 199365..199955
/gene="Tsa1"
/locus_tag="R103_M10806"
/note="Similar to YML028W 'Thioredoxin peroxidase, acts as
both a ribosome-associated and free cytoplasmic
antioxidant; self-associates to form a high-molecular
weight chaperone complex under oxidative stress; deletion
results in mutator phenotype'"
/codon_start=1
/product="Tsa1p"
/protein_id="EWG94323.1"
/translation="MVAQVQKQAPTFKKTAVVDGVFDEVSLDKYKGKYVVLAFIPLAF
TFVCPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINFPLLA
DTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHITINDLPVGRNVDEALRLVEAFQ
WTDKNGTVLPCNWTPGAATIKPTVEDSKEYFEAANK"
gene 200633..201790
/gene="Yox1"
/locus_tag="R103_M10816"
mRNA 200633..201790
/gene="Yox1"
/locus_tag="R103_M10816"
/product="Yox1p"
CDS 200633..201790
/gene="Yox1"
/locus_tag="R103_M10816"
/note="Similar to YML027W 'Homeodomain-containing
transcriptional repressor, binds to Mcm1p and to early
cell cycle boxes (ECBs) in the promoters of cell
cycle-regulated genes expressed in M/G1 phase; expression
is cell cycle-regulated; potential Cdc28p substrate'"
/codon_start=1
/product="Yox1p"
/protein_id="EWG94324.1"
/translation="MSQETKMLPSLSSLLSGTEISSSPVSPSFTNPRTSFHLDDRGTI
KLPPLNTSINRPRSVESALRHTVTSLHENSSAYGDDMLKHTQSDSALSSQLDSSQETV
DESHENLLLTPLNSKKRDYSVSSKKNDILTPLSAAKSIIIPSASKEKRRAFAFITHSQ
ETFPKKEPKIDNAPLARRKRRRTSSQELSILQAEFEKCPAPSKEKRIELAESCHMTEK
AVQIWFQNKRQAVKRQRIATSKSTTIIQTVSPPSPPLDVHATPLASRVKADILRDGSS
CSRSSSSSPLENTPPRPHHSLNRRSSTPSIKRSQALTFHLNPQKKTLTPVKTSPNSRV
NKLINSIDHSPSKAKRPVSNPSGSPKRKRKFGFKIVDQQPLKDLDPNAFRG"
gene complement(202214..203055)
/gene="Rps18b"
/locus_tag="R103_M10821"
mRNA complement(join(202214..202607,203009..203055))
/gene="Rps18b"
/locus_tag="R103_M10821"
/product="Rps18bp"
CDS complement(join(202214..202607,203009..203055))
/gene="Rps18b"
/locus_tag="R103_M10821"
/note="Similar to YML026C 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps18Ap and
has similarity to E. coli S13 and rat S18 ribosomal
proteins'"
/codon_start=1
/product="Rps18bp"
/protein_id="EWG94325.1"
/translation="MSLVVQEQGSFQHILRLLNTNVDGNIKIVYALTTIKGVGRRYSN
LVCKKADVDLHKRAGELTQEELERIVQIMQNPTHYKIPAWFLNRQNDITDGKDYHTLA
NNVESKLRDDLERLKKIRAHRGIRHFWGLRVRGQHTKTTGRRRA"
gene complement(203632..204591)
/gene="Yml6"
/locus_tag="R103_M10826"
mRNA complement(join(203632..204465,204565..204591))
/gene="Yml6"
/locus_tag="R103_M10826"
/product="Yml6p"
CDS complement(join(203632..204465,204565..204591))
/gene="Yml6"
/locus_tag="R103_M10826"
/note="Similar to YML025C 'Mitochondrial ribosomal protein
of the large subunit, has similarity to E. coli L4
ribosomal protein and human mitoribosomal MRP-L4 protein;
essential for viability, unlike most other mitoribosomal
proteins'"
/codon_start=1
/product="Yml6p"
/protein_id="EWG94326.1"
/translation="MTIKRNLVKTLQSIRYQATTATAHAESTLSPLPNAAIPPKYALV
TVRSFPSLEPLTFVPVPTSTVAAPLRRDILWRAVVYENDNRRVGASNPPGRSENGFSR
RKLMPQKGSGRARVGDANSPTRHNGGRALARTAPNDYTTELPSKVYSMAFNNALSHQY
KSGKLFVIGGEKVDLISPTPELDLNRLDLVNTNTVEGKEIFEGEVIFRKFLEEFQLKG
KRLLFITDKTREGLIKSSDPYKQKVDVIQKELVEVNDILRAQAVFIELEALEYLAMAH
QKEILHSVSN"
gene 205114..205881
/gene="Rps17a"
/locus_tag="R103_M10831"
mRNA join(205114..205116,205492..205881)
/gene="Rps17a"
/locus_tag="R103_M10831"
/product="Rps17ap"
CDS join(205114..205116,205492..205881)
/gene="Rps17a"
/locus_tag="R103_M10831"
/note="Similar to YML024W 'Ribosomal protein 51 (rp51) of
the small (40s) subunit; nearly identical to Rps17Bp and
has similarity to rat S17 ribosomal protein'"
/codon_start=1
/product="Rps17ap"
/protein_id="EWG94327.1"
/translation="MTVKRASKALIERYYPKLTLDFQTNKRLCDEIATIQSKRLRNKI
AGYTTHLMKRIQKGPVRGISFKLQEEERERKDQYVPEVSALDLSRSNGVLNVDNQTSD
LVKSLGLKLPLSVINVSAQRDRRYRKRV"
gene complement(206182..207852)
/gene="Nse5"
/locus_tag="R103_M10836"
mRNA complement(206182..207852)
/gene="Nse5"
/locus_tag="R103_M10836"
/product="Nse5p"
CDS complement(206182..207852)
/gene="Nse5"
/locus_tag="R103_M10836"
/note="Similar to YML023C 'Essential subunit of the
Mms21-Smc5-Smc6 complex; required for cell viability and
DNA repair'"
/codon_start=1
/product="Nse5p"
/protein_id="EWG94328.1"
/translation="MDGALINSVLYVSPRNGAHYFVELTEKHLLAFEMLNSMCLLENY
DHVLLFLECQFGKSHNLAVIPFDIILVLFTLSTLSEYYKEPILRANDPYNTSRETLSR
RALKLLQKYLAILKEFDSEQYNLYDLELLRCQFFLAIDTLTPKKQKWSFDRFRRTKSE
KGVTYRQNASVDPELDQAKTFKNPYRSYISCLEQRNTILGNRLLNLKLNEPGEFINMI
LWTLSNSLQESTPLFLSSHEIWMPLLEILIDLFSCRQDYFIQHEVAQNVSKSLFVQRL
SESPLAVFFESLNTRNFANRFSEYVFLNCDYKLPSDNYATPVHPVYNGENTIVDTYIP
TIKCSPLYKSQKSLALRRKLIGSCFKLLLRVPDGHRLITPRIVADDVIQGISRTLASF
NDILQFKKFFMTENLSQESYFIPLLAEGTLSEILKDTQECVVILTLVENLSDGVSFCN
EVIGLVKSKCFAFTEQCSQTSYEEAVLNIEKCDVCLLVLLRYLLHLIGTEAILDAKEQ
LEMLHAIEKNDSGRRQWAKALNLGNNPPLLYPIVSQMFGVHDKSVIIE"
gene 208125..208688
/gene="Apt1"
/locus_tag="R103_M10846"
mRNA 208125..208688
/gene="Apt1"
/locus_tag="R103_M10846"
/product="Apt1p"
CDS 208125..208688
/gene="Apt1"
/locus_tag="R103_M10846"
/note="Similar to YML022W 'Adenine
phosphoribosyltransferase, catalyzes the formation of AMP
from adenine and 5-phosphoribosylpyrophosphate; involved
in the salvage pathway of purine nucleotide biosynthesis'"
/codon_start=1
/product="Apt1p"
/protein_id="EWG94329.1"
/translation="MSIASYAQELKLALHQYPNFPSEGILFEDFLPIFRNPGLFQKLI
DAFKLHLEEAFPEVKIDYIVGLESRGFLFGPTLALALGVGFVPVRKAGKLPGECFKAT
YEKEYGSDLFEIQKNAIPAGSNVIIVDDIIATGGSAAAAGGLVEQLEANLLEYNFVME
LDFLKGRSKLNAPVFTLLNAQKEALKK"
gene 212614..213612
/gene="Ost6"
/locus_tag="R103_M10861"
mRNA 212614..213612
/gene="Ost6"
/locus_tag="R103_M10861"
/product="Ost6p"
CDS 212614..213612
/gene="Ost6"
/locus_tag="R103_M10861"
/note="Similar to YML019W 'Subunit of the
oligosaccharyltransferase complex of the ER lumen, which
catalyzes asparagine-linked glycosylation of newly
synthesized proteins; similar to and partially
functionally redundant with Ost3p'"
/codon_start=1
/product="Ost6p"
/protein_id="EWG94330.1"
/translation="MKWCSTYIIIWLAIIFHKFQKSTATASHNIDDILQLKGDTGVIT
VTADNYPLLSRGVPGYFNILYITMRGTNSNGMSCQLCHDFEKTYQAVADVIRSQAPQS
LNLFFTVDVNEVPQLVKDLKLQNVPHLVVYPPAESNKQSQFEWKTSPFYQYSLVPENA
ENTLQFGDFLAKILNISITVPQAFNVQEFVYYFVACMVVFIFIKKVILPKVTNKWKLF
SMILSLGILLPSITGYKFVEMNAIPFIARDAKNRIMYFSGGSGWQFGIEIFSVSLMYI
VMSALSVLLIYVPKISCVSEKMRGLLSSFLACVLFYFFSYFISCYLIKNPGYPIVF"
gene complement(213927..215108)
/locus_tag="R103_M10871"
mRNA complement(213927..215108)
/locus_tag="R103_M10871"
/product="hypothetical protein"
CDS complement(213927..215108)
/locus_tag="R103_M10871"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94331.1"
/translation="MVSKDQTSFNKRWTLGLLMLGLVIILWVLSSFLINLIFEDDSYR
KPFFITYINTAAFIFYLFPTAKAVVVNYKDTGRANVHRELIMEEEGTGSDSNRSVDMT
SPLLTNLEAGTHANQKKRLTLYETIKLSAEFCILWFTANLVTNASLAFTSVASQTILS
TTSSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRHIADVSGDDN
DAVRVLIGNLLALAGAVLYGVYSTLLKREVGDETRVNMKIFFGFVGLFNLLFLWPSLI
VLDFFGWEPFSLPKDPKVVVIIFVNCLITFVSDFCWAKAMLLTSPLTVTVGLSITIPL
AMFGDVIFKHKTMSALYLFGATLILGSFFIINKSSEEEHFENSITASNYESVEVPAAN
N"
gene 215744..217880
/gene="Psp2"
/locus_tag="R103_M10876"
mRNA join(215744..215747,216109..217880)
/gene="Psp2"
/locus_tag="R103_M10876"
/product="Psp2p"
CDS join(215744..215747,216109..217880)
/gene="Psp2"
/locus_tag="R103_M10876"
/note="Similar to YML017W 'Asn rich cytoplasmic protein
that contains RGG motifs; high-copy suppressor of group II
intron-splicing defects of a mutation in MRS2 and of a
conditional mutation in POL1 (DNA polymerase alpha);
possible role in mitochondrial mRNA splicing'"
/codon_start=1
/product="Psp2p"
/protein_id="EWG94332.1"
/translation="MGTNNTSNNNGTTKKMSLEEFLGNDTLGESVWDEEDINLDAISN
TTNIDILKQTKAGEHQRDGHQQHPHGGHGPMNRSRFSNAGPFGGGSMGDFANHHHPLQ
HQQGPPYIVKFSDLPPRFSNFDIEDLFQAKFTKFIKFKLFWEINKNPSISTLKSGSIF
DQNFKRDSKVAFVELYTSRDMDKILNYWTTPLKEIYHITTAPAEFEDFKDYSTKVKLL
TDPKDDAGKPFITKTQRSKSNPFGSAKPVDTQSKILDIEEKMENLHVEDTTTLRASLI
PSSDSMATTATGSKITILKKQTPTEEESHSATPTPKPLSYSEVVERSVVNETSKKGTP
LSKLGSPALELQSKPDKSDEFKGGDEQGFEKGGDDKAQLDVSNDKDKGSETDVDKQFT
FKNVEREHSMSRTKYNGNHNNNNGNFRGSNRYRGGPNGSSYKGGHNNRGNRGGYRSGS
SYNNNTNDNNNNNNNNSSSNNNNGSRYHDRQNNEEGLTSDSSLDASGNKKNDFTNSTS
NTQQYSIFKPASGFLGQGNNDSIRNNGRGNYNSSGMNGGSRGRGFGRGRGFGRGAYNN
RGSRGGRGSSGNYSNYNNRTTDMPL"
assembly_gap 218208..218233
/estimated_length=26
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(218567..220645)
/gene="Ppz1"
/locus_tag="R103_M10891"
mRNA complement(218567..220645)
/gene="Ppz1"
/locus_tag="R103_M10891"
/product="Ppz1p"
CDS complement(218567..220645)
/gene="Ppz1"
/locus_tag="R103_M10891"
/note="Similar to YML016C 'Serine/threonine protein
phosphatase Z, isoform of Ppz2p; involved in regulation of
potassium transport, which affects osmotic stability, cell
cycle progression, and halotolerance'"
/codon_start=1
/product="Ppz1p"
/protein_id="EWG94333.1"
/translation="MGNSSSKSSKKDSHSNSSSRNPRPQVSRTETSHSVKSAKSNKSS
RSRRSLPSSSTTNTNSNVPDPSTPSKPNLEVNHQRHSSHTNRYHFPSSSHSHSNSQNE
LLTTPSSSSTKRPSTSRRSSYNTKAAADLPPSMIQMEPKSPILKTNNSSTHVSKHKSS
YSSTYYENALTDDDNDDKDNDISHTKRFSRSSNSRPSSIRSGSVSRRKSDVTHEEPNN
GSYSSNNQENYLVQALTRSNSHASSLHSRKSSFGSDGNTAYSTPLNSPGLSKLTDHSG
EYFTSNSTSSLNHHSSRDIYPSKHISNDDDIENSSQLSDIHASMENVNDKNNNITDSK
KDPNEEFNDIMQSSGNKNAPKKFKKPIDIDETIQKLLDAGYAAKRTKNVCLKNNEILQ
ICIKAREIFLSQPSLLELSPPVKIVGDVHGQYGDLLRLFTKCGFPPSSNYLFLGDYVD
RGKQSLETILLLFCYKIKYPENFFLLRGNHECANVTRVYGFYDECKRRCNIKIWKTFI
DTFNTLPLAAIVAGKIFCVHGGLSPVLNSMDEIRHVVRPTDVPDFGLINDLLWSDPTD
SPNEWEDNERGVSYCYNKVAINKFLNKFGFDLVCRAHMVVEDGYEFFNDRSLVTVFSA
PNYCGEFDNWGAVMSVSEGLLCSFELLDPLDSAALKQVMKKGRQERKLANQQQQMMET
SITNDNESQQ"
gene complement(221103..222143)
/gene="Taf11"
/locus_tag="R103_M10911"
mRNA complement(221103..222143)
/gene="Taf11"
/locus_tag="R103_M10911"
/product="Taf11p"
CDS complement(221103..222143)
/gene="Taf11"
/locus_tag="R103_M10911"
/note="Similar to YML015C TFIID subunit (40 kDa), involved
in RNA polymerase II transcription initiation, similar to
histone H3 with atypical histone fold motif of Spt3-like
transcription factors"
/codon_start=1
/product="Taf11p"
/protein_id="EWG94334.1"
/translation="MTEPQGPLDTIPKVNYPPILTIANYFSTKQMIDQVISEDQDYVT
WKLQNLRTGGTSINNQLNKYPKYKYQKTRINQQDLDSINKVPENLIFPQDILQQQTQN
SNYEDTNTNEDENEKLAQDEQFKLLVTNLDKDQTNRFEVFHRTSLNKTQVKKLASTVA
NQTISENIRVFLQAVGKIYAGEIIELAMIVKNKWLTSQMCIEFDKRTKIGYKLKKYLK
KLTFSIIENQQYKQDYQSDSVPEDEPDFYFDDEEVDKRESTLGNSLLQSKSLQQSDHN
SQDLKLQLIEQYNKLVVQFNKLDVSIEKYNNSPLLPEHIREAWRLYRLQSDTLPNAYW
RTQGEGQGSMFR"
gene 222339..223178
/gene="Trm9"
/locus_tag="R103_M10916"
mRNA 222339..223178
/gene="Trm9"
/locus_tag="R103_M10916"
/product="Trm9p"
CDS 222339..223178
/gene="Trm9"
/locus_tag="R103_M10916"
/note="Similar to YML014W 'tRNA methyltransferase,
catalyzes esterification of modified uridine nucleotides
in tRNA(Arg3) and tRNA(Glu), likely as part of a complex
with Trm112p; deletion confers resistance to zymocin'"
/codon_start=1
/product="Trm9p"
/protein_id="EWG94335.1"
/translation="MEINQAAEKEQEYVHKVYNEIAPHFSQTRYKPWPIVTQFLKTRP
MGSIGIDVGCGNGKYLGVNPDIYIIGSDRSDGLIECARGINPSYNLLVADGLNLPHKN
ETFDFAISIAVVHHWSTRERRVEVIRHVLSKLRQGGQALIYCWALEQGSSRRGYHEGM
EQDVFVPWVLPKSKSKPKTKSTPPSKVKTRPKPNLMNIPPKERSEYLQRWKEEQQRSK
SLDDNDEKQQQDQEQEREEVKYRYYHLYREGELAEDCRQAGAAVHSEGFERDNWWVVA
QKR"
gene 223263..225017
/gene="Ubx2"
/locus_tag="R103_M10921"
mRNA 223263..225017
/gene="Ubx2"
/locus_tag="R103_M10921"
/product="Ubx2p"
CDS 223263..225017
/gene="Ubx2"
/locus_tag="R103_M10921"
/note="Similar to YML013W 'Protein involved in
ER-associated protein degradation; proposed to coordinate
the assembly of proteins involved in ERAD; contains a UBX
(ubiquitin regulatory X) domain and a ubiquitin-associated
(UBA) domain'"
/codon_start=1
/product="Ubx2p"
/protein_id="EWG94336.1"
/translation="MPVVNHEDSEFHLSHTEEDKLNEFQVITNFPPGDLPDVVRLLRN
HGWQLEPALSRYFDGEWKGEPDQMGESTQTSTPMAETLVPPALGPRPLLFTASLPVVR
PLPANFRNDFRTIGLNGRSNTVWSMFESFSYDGNPFLFILLLIPRIINRLSATIFTFF
CTLLSLHSISGGGNSGKPKISKVPKAPTRETHIPLAEILGDTKDKDAFCELKSFKPDI
SFNEALRIAKEEFKFMLLILVGDTYDTDTDTVDVNSKLLLEKILLNKKTLQYLRKIDN
DLIIYLKCVHELEPWLVARQLGVRNTPEIFLIANVANKASHSETLPSQRLSILGKLKV
NSLNRFLQSLTNVVEKYTPELVVNKTEMHELRMSREIKKLQEDAYKKSLEMDRIKAIE
KEKSLKHAQDLKLNSTARQLKWLKACIDEIQPFETTGKQATLQFRTSSGKRFVKKFPS
MTTLYQIYQSIGCHIYLAVYSSDPVEWSNALQDKIRQLSADDDVLCFKEGQLETATAT
TIEELGHIINNELTSFDLERGKLEFDFELVSPFPKYTVHPNEHMSVDQVPQLWPNGSL
LVEALDEEDEEDEENEEQ"
gene complement(224651..225028)
/locus_tag="R103_M10926"
mRNA complement(224651..225028)
/locus_tag="R103_M10926"
/product="hypothetical protein"
CDS complement(224651..225028)
/locus_tag="R103_M10926"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94337.1"
/translation="MLFYHCSSFSSSSSSSSSSASTRRLPFGHSWGTWSTDMCSLGCT
VYLGKGDTNSKSNSSLPRSRSNEVSSLLMMWPSSSIVVAVAVSSCPSLKQSTSSSADN
CLILSWRALDHSTGSLEYTARYM"
gene 225230..225865
/gene="Erv25"
/locus_tag="R103_M10931"
mRNA 225230..225865
/gene="Erv25"
/locus_tag="R103_M10931"
/product="Erv25p"
CDS 225230..225865
/gene="Erv25"
/locus_tag="R103_M10931"
/note="Similar to YML012W Protein that forms a
heterotrimeric complex with Erp1, Erp2p, and Emp24, member
of the p24 family involved in endoplasmic reticulum to
Golgi transport"
/codon_start=1
/product="Erv25p"
/protein_id="EWG94338.1"
/translation="MQVLQLWLTTLISLVVAVQGLHFDIAASTDPEQVCIRDFVTEGQ
LVVVDIHSDGSVGDGQKLNLFVRDSVGNEYRRKRDFAGDVRVAFTAPSSTAFDVCFEN
QAQYRGRSLSRAIELDIESGAEARDWNKISANEKLKPIEVELRRVEEITDEIVDELTY
LKNREERLRDTNESTNRRVRNFSILVIIVLSSLGVWQVNYLKNYFKTKHII"
gene complement(226009..226542)
/gene="Rad33"
/locus_tag="R103_M10936"
mRNA complement(226009..226542)
/gene="Rad33"
/locus_tag="R103_M10936"
/product="Rad33p"
CDS complement(226009..226542)
/gene="Rad33"
/locus_tag="R103_M10936"
/note="Similar to YML011C 'Protein involved in nucleotide
excision repair; green fluorescent protein (GFP)-fusion
protein localizes to the nucleus'"
/codon_start=1
/product="Rad33p"
/protein_id="EWG94339.1"
/translation="MSKSTNVSYERVELFENPKVPIEVEDEILEKYAESSLDHDMTVN
ELPRFFKDLQLEPTIWKLVRNEDVIIEGTDVIDFTKLVRCTCQLLILMNNLTVIDDLW
SMLIRNCGRDVDFPQVALRDHVLSVKDLQKISNLIGADQSSGTIEMISCATDGKRLFM
TYLDFGCVLGKLGYLKM"
gene 226789..229979
/gene="Spt5"
/locus_tag="R103_M10941"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 229546..230022
/locus_tag="R103_M10946"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(229786..230111)
/locus_tag="R103_M10951"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 230145..230198
/estimated_length=54
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(230415..230627)
/gene="Mrpl39"
/locus_tag="R103_M10956"
mRNA complement(230415..230627)
/gene="Mrpl39"
/locus_tag="R103_M10956"
/product="Mrpl39p"
CDS complement(230415..230627)
/gene="Mrpl39"
/locus_tag="R103_M10956"
/note="Similar to YML009C Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl39p"
/protein_id="EWG94340.1"
/translation="MVKVKSKNSVIKLLSTAASGYSRYISIKKGAPLVTQVRYDPVVK
RHVLFKEAKKRKVAERKPLDFLRTAK"
gene complement(230955..232106)
/gene="Erg6"
/locus_tag="R103_M10961"
mRNA complement(230955..232106)
/gene="Erg6"
/locus_tag="R103_M10961"
/product="Erg6p"
CDS complement(230955..232106)
/gene="Erg6"
/locus_tag="R103_M10961"
/note="Similar to YML008C 'Delta(24)-sterol
C-methyltransferase, converts zymosterol to fecosterol in
the ergosterol biosynthetic pathway by methylating
position C-24; localized to both lipid particles and
mitochondrial outer membrane'"
/codon_start=1
/product="Erg6p"
/protein_id="EWG94341.1"
/translation="MSETELRKRQAQFTRELHGDDIGKKTGLSALMSKNNSAQKEAVQ
KYLRNWDGRTDKDAEERRLEDYNEATHSYYNVVTDFYEYGWGSSFHFSRFYKGESFAA
SIARHEHYLAYKAGIQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIAKAKY
YAKKYNLSDQMDFVKGDFMKMDFEENTFDRVYAIEATCHAPKLEGVYSEIYKVLKPGG
TFAVYEWVMTDKYDENNPEHRKIAYEIELGDGIPKMFHVDVARKALKNCGFEVLVSED
LADNDDEIPWYYPLTGEWKYVQNLANLATFFRTSYLGRQFTTAMVTVMEKLGLAPEGS
KEVTAALENAAVGLVAGGKSKLFTPMMLFVARKPENAETPSQTSQEATQ"
gene complement(232278..232388)
/locus_tag="R103_M10971"
mRNA complement(232278..232388)
/locus_tag="R103_M10971"
/product="hypothetical protein"
CDS complement(232278..232388)
/locus_tag="R103_M10971"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94342.1"
/translation="MVHFIFIALRSMRFMRRLVRNLQYLLLPITSSLLFI"
gene 232964..234934
/gene="Yap1"
/locus_tag="R103_M10981"
mRNA 232964..234934
/gene="Yap1"
/locus_tag="R103_M10981"
/product="Yap1p"
CDS 232964..234934
/gene="Yap1"
/locus_tag="R103_M10981"
/note="Similar to YML007W 'Basic leucine zipper (bZIP)
transcription factor required for oxidative stress
tolerance; activated by H2O2 through the multistep
formation of disulfide bonds and transit from the
cytoplasm to the nucleus; mediates resistance to cadmium'"
/codon_start=1
/product="Yap1p"
/protein_id="EWG94343.1"
/translation="MSVSTAKRSLDVVSPGSLAEFEGSKSRHDEIENEHRRTGTRDGE
DSEQPKKKGSKTSKKQDLDPETKQKRTAQNRAAQRAFRERKERKMKELEKKVQSLESI
QQQNEVEATFLRDQLITLVNELKKYRPETRNDSKVLEYLARRDPNLLFSKNNVNHSNS
EPIDTPNDDIQENVKQKMNFTFQYPLDNDNDNDDSKNVGKQLPSPNDPSHSAPMPINQ
TQKKLSDATDSSSATLDSLSNSNDVLNNTPNSSTSMDWLDNVIYTNRFVSGDDGSNSK
TKNLDSNMFSNDFNFENQFDEQVSEFCSKMNQVCGTRQCPIPKKPISALDKEVFASSS
ILSSNSPALTNTWESHSNITDNTPANVIATDATKYENSFSGFGRLGFDMSANHYVVND
NSTGSTDSTDSTGSTGSTGNKNKKNNNNSDDVLPFISESPFDMNQVTNFFSPGSTGIG
NNAASNTNPSLLQSSKEDIPFINANLAFPDDNSTNIQLQPFSESQSQNKFDYDMFFRD
SSKEGNNLFGEFLEDDDDDKKAANMSDDESSLIKNQLINEEPELPKQYLQSVPGNESE
ISQKNGSSLQNADKINNGNDNDNDNDVVPSKEGSLLRCSEIWDRITTHPKYSDIDVDG
LCSELMAKAKCSERGVVINAEDVQLALNKHMN"
gene 239312..240700
/gene="Trm12"
/locus_tag="R103_M11001"
mRNA 239312..240700
/gene="Trm12"
/locus_tag="R103_M11001"
/product="Trm12p"
CDS 239312..240700
/gene="Trm12"
/locus_tag="R103_M11001"
/note="Similar to YML005W 'S-adenosylmethionine-dependent
methyltransferase of the seven beta-strand family;
required for wybutosine formation in
phenylalanine-accepting tRNA'"
/codon_start=1
/product="Trm12p"
/protein_id="EWG94344.1"
/translation="MSDETMPVEFLVSDKRLLKTIKVKLETNGLFVTPIYSDNDNKVI
KSSIEDLNHPLAVEINNIAGVKARFHESSNLERSEGRLKHQSNSITEFTKSFLKDHGL
ANDKIFLSHLLDHLPLKYTIYPPVVLFNNSTVRSFNHPIWQKAFQLKLFDPNEYYREL
LCFLSPGKPSKGTSLHPNNRLLTHLAINNPITEADVLRRPFNIQPLYGKLIDDSILDD
NDNTLWENPSQEQLNSSIWCKVIQNGVTQIWSPVFTMFSRGNIKEKKRVLTTFPDICN
NDVVDLYAGIGYFTFSYLTKGARTLFAFELNPWSVEGLKRGLKANGFSKSGNCHVFQE
SNEMCVQRLTEFLSQNPGFRLRIRHINLGLLPSSKQGWPLAIKLIYLQGASLEKVTMH
IHENVHIDAIEDGSFEKNVIVELDAINESIALIRNRGIKLQFVRSKLERIKTFAPDIW
HVCVDVDVIVST"
gene complement(240796..241776)
/gene="Glo1"
/locus_tag="R103_M11006"
mRNA complement(240796..241776)
/gene="Glo1"
/locus_tag="R103_M11006"
/product="Glo1p"
CDS complement(240796..241776)
/gene="Glo1"
/locus_tag="R103_M11006"
/note="Similar to YML004C 'Monomeric glyoxalase I,
catalyzes the detoxification of methylglyoxal (a
by-product of glycolysis) via condensation with
glutathione to produce S-D-lactoylglutathione; expression
regulated by methylglyoxal levels and osmotic stress'"
/codon_start=1
/product="Glo1p"
/protein_id="EWG94345.1"
/translation="MSTDSTRYPIQIEKASNDPTLLLNHTCLRVKDPARAVKFYTEHF
GMKLLSRKDFEEAKFSLYFLSFPKDDIPKNKNGEPDVFSAHGVLELTHNWGTEKNPDY
KINNGNEEPHRGFGHICFSVSDINKTCEELESQGVKFKKRLSEGRQKDIAFALDPDGY
WIELITYSREGQEYPKGSVGNKFNHTMIRIKNPTRSLEFYQNVLGMKLLRTSEHESAK
FTLYFLGYGVPKTDSVFSCESVLELTHNWGTENDPNFHYHNGNSEPQGYGHICISCDD
AGALCKEIEVKYGDKIQWSPKFNQGRMKNIAFLKDPDGYSIEVVPYGLIA"
gene 243636..245849
/locus_tag="R103_M11011"
mRNA 243636..245849
/locus_tag="R103_M11011"
/product="hypothetical protein"
CDS 243636..245849
/locus_tag="R103_M11011"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG94346.1"
/translation="MDSHQLELPDGLNNMTMDADTLISLFVLVVCRSEQKHLKSHLYY
LQNFSNNSSSTKFGILGYAVSTLEAVVCYFEDFNKNTGNVAKANTLCEKTKNLLDKLS
CENPTNEVEDLATYKDILTYRNEQGQSILSICITNHKNYILLDILSEYETDFPVEDLL
EDETIDGSTLLIESIKAGNLEAAKVLIKIMLFNCTEEELVSYINKTDKYARTVAHYLT
HEMDILKSIGNYIDWKRKNSSGQTPLFSIFRSYDQPNYEEMVKTAFDIANTWYRKHNS
LFDYLDHTDNKGNSLLHVLKTNIPILLQLTKLDINEENYKGLTPLMVYVKYKRLSNID
AITKDRRLILEKVQNSTFFTCFDYAKDHSVLSKIGERGVKDSLFGLIYFHSLRYHNLN
ATTNITSVSNAEKPFATTVINMKTIQGLLRSILKDNPFTFLPLNTYIDEISHLNRSDL
TIIGKTDVTSLLHQLTNCFNVLLFLKKIPENLFTDEASILYWMRINTSKRNQKPSGKE
NPKTMEPEEINMIQSFLRFNFDEISSFKASLNILRKVLIFINLKSDDFEDAYKGLNEM
GRKLINSEASSAFKGIITNHNMFSELSLAELLENVRFLEQCTIQLSSFVQIILFEKIP
NWWKHYGEFLALHKSYRKAFPNMVKPKSASDTSSRAPLGGFIETKREQSEQRLAVQIK
ASSKMLKELGSEIFVAHERLAEELSNYMEFRKACLDQRSLVAFATTNISVLQECV"
gene 246276..246862
/gene="Ypt7"
/locus_tag="R103_M11021"
/note="nonfunctional due to frameshift determined by
sequencing error"
CONTIG join(APIR01000342.1:1..35524,gap(124),APIR01000343.1:1..50244,
gap(15),APIR01000344.1:1..63657,gap(11),APIR01000345.1:1..7028,
gap(20),APIR01000346.1:1..3273,gap(339),APIR01000347.1:1..14605,
gap(13),APIR01000348.1:1..2148,gap(20),APIR01000349.1:1..41186,
gap(26),APIR01000350.1:1..11911,gap(54),APIR01000351.1:1..19625)
//