LOCUS KK037074 882485 bp DNA linear CON 11-FEB-2014
DEFINITION Saccharomyces cerevisiae P301 chromosome XIII genomic scaffold
scaffold23, whole genome shotgun sequence.
ACCESSION KK037074 APIQ01000000
VERSION KK037074.1
DBLINK BioProject: PRJNA162717
BioSample: SAMN01915094
KEYWORDS WGS.
SOURCE Saccharomyces cerevisiae P301
ORGANISM Saccharomyces cerevisiae P301
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 882485)
AUTHORS Treu,L., Toniolo,C., Nadai,C., Sardu,A., Giacomini,A., Corich,V.
and Campanaro,S.
TITLE The impact of genomic variability on gene expression in
environmental Saccharomyces cerevisiae strains
JOURNAL Environ. Microbiol. (2013) In press
PUBMED 24238297
REMARK Publication Status: Available-Online prior to print
REFERENCE 2 (bases 1 to 882485)
AUTHORS Campanaro,S., Treu,L. and Corich,V.
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2013) Department of Biology, University of Padua,
Via U. Bassi 58/b, Padova, PD 35121, Italia
COMMENT ##Genome-Assembly-Data-START##
Assembly Method :: Newbler v. 2.3
Assembly Name :: p301
Genome Coverage :: 14.0x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..882485
/organism="Saccharomyces cerevisiae P301"
/mol_type="genomic DNA"
/submitter_seqid="scaffold23"
/strain="P301"
/isolation_source="grape bunch from Prosecco vineyard"
/db_xref="taxon:1182968"
/chromosome="XIII"
/country="Italy: Col De Roer (TV)"
/collection_date="Sep-2004"
gene 557..1654
/locus_tag="P301_M10006"
mRNA 557..1654
/locus_tag="P301_M10006"
/product="hypothetical protein"
CDS 557..1654
/locus_tag="P301_M10006"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89191.1"
/translation="MVLAKQWVLKNLPTPGEPFNFHFHDPACTFELIEKELSSEQLKD
GELLLETTYLSNDPAQKFWISSMDKNYAKGVQPGEIIPARGIGKVLASRNKAFSPGDY
VSAVTGWTTHAIISQENVQGLRKLDKNKVGKLWWYLSVLGGTSLTAYFIFFTYAQLQE
REEDYGKVYLISGAAGAVGTVCIQLALNVFKASKVIAIAGGPEKVAFVESFGDNVVGV
DYKDPSFKQKLIEAAGGENTVDYFIDNVGSNVLEAGVLLLKQRAMLIACGAISAYNDP
SKFVFKGYSFILTKRLVVKGVLVTDNIDDFPKALDKLGSLVKHGKIDLLKSATLEDGT
GDKFKNVPLIWKGLFSGVNKGKLITKVNNEE"
gene complement(1842..3533)
/gene="Ero1"
/locus_tag="P301_M10011"
mRNA complement(1842..3533)
/gene="Ero1"
/locus_tag="P301_M10011"
/product="Ero1p"
CDS complement(1842..3533)
/gene="Ero1"
/locus_tag="P301_M10011"
/note="Similar to YML130C Thiol oxidase required for
oxidative protein folding in the endoplasmic reticulum"
/codon_start=1
/product="Ero1p"
/protein_id="EWG89192.1"
/translation="MRLRTAIATLCLTAFTSATSNNSYIATDQTQNAFNDTHFCKVDR
NDHVSPSCNVTFNELNAINENIRDDLSALLKSDFFKYFRLDLYKQCSFWDANDGLCLN
RACSVDVVEDWDTLPEYWQPEILGSFNNDTMKEADDSDDECKFLDQLCQTSKKPVDIE
DTINYCDVNDFNGKNAVLIDLTANPERFTGYGGKQAGQIWSTIYQDNCFTIGETGESL
AKDAFYRLVSGFHASIGTHLSKEYLNTKTGKWEPNLDLFMARIGNFPDRVTNMYFNYA
VVAKALWKIQPYLPEFSFCDLVNKEIKNKMDNVISQLDTKIFNEDLVFANDLSLTLKD
EFRSRFKNVTKIMDCVQCDRCRLWGKIQTTGYATALKILFEINDADEFTKQHIVGKLT
KYELIALLQTFGRLSESIESVNMFEKMYGKRLNGSENRLSSFFQNNFFNILKEAGKSI
RYTIENINSTKEGKKKTNNSQSHVFDDLKMPKAEIVPRPSNGTVNKWKKAWNTEVNNV
LEAFRFIYRSYLDLPRNIWELSLMKVYKFWNKFIGVADYVSEETREPISYKLDIQ"
gene complement(4899..5111)
/gene="Cox14"
/locus_tag="P301_M10021"
mRNA complement(4899..5111)
/gene="Cox14"
/locus_tag="P301_M10021"
/product="Cox14p"
CDS complement(4899..5111)
/gene="Cox14"
/locus_tag="P301_M10021"
/note="Similar to YML129C 'Mitochondrial membrane protein,
involved in translational regulation of Cox1p and assembly
of cytochrome c oxidase (complex IV); associates with
complex IV assembly intermediates and complex III/complex
IV supercomplexes'"
/codon_start=1
/product="Cox14p"
/protein_id="EWG89193.1"
/translation="MSKYAWYTRVTDTLHRLTVLTLVGGTLYMSGGLAYTLYMNGKKY
EQQVTQQKALEEDNQQLQSPTAPPTE"
gene complement(5493..7034)
/gene="Msc1"
/locus_tag="P301_M10031"
mRNA complement(5493..7034)
/gene="Msc1"
/locus_tag="P301_M10031"
/product="Msc1p"
CDS complement(5493..7034)
/gene="Msc1"
/locus_tag="P301_M10031"
/note="Similar to YML128C 'Protein of unknown function;
mutant is defective in directing meiotic recombination
events to homologous chromatids; the authentic, non-tagged
protein is detected in highly purified mitochondria and is
phosphorylated'"
/codon_start=1
/product="Msc1p"
/protein_id="EWG89194.1"
/translation="MKQFKLVNAVSASFVLIGLVLANSDSVFDKWTQEDLADYLRDNK
KSLEKYATDSIEDLKKEASQVWDKHAQPKPWWQVWSSDSSSVSNSNPGWFGYTGSSDH
PVSDWLFDTWSTDSLRNFLKKNGVDVDDAKASKDSLVKTAKENFNKISKSLKSSGYYP
SSSYFDSWSTKDLQNWLNDNGIDYDKAVQSKDELVQKVKENIYRTSEKAEQQRLGLLE
SLDLAHQQILDTSGQIKDTVFDKWSSDQLTNWLESHKVNIDKNMAKKHDYLVRMAKEN
SANLKDDIYWYLDYMKRESSPFLTKTPEYVGSVWDSSKNFLTNLYSKFRGKTDNVIND
TFLVGLDSWPKDKLKMFLDARGIKYSMLSTEHQLRELVKKSRNEKLKILPKDYQKYFD
NSNWSLDDIKGWFADKKDDFQDSQTYSTIMQDFDKVSKNTNDAKDQIAKTWSNTFQSW
SQEDLLQYLKSFGVPVKQTSTKDDLINLAKQNTQWLFGTVKEPAYKRYLHNVKNWSKS
ILGFN"
gene 7422..9167
/gene="Rsc9"
/locus_tag="P301_M10036"
mRNA 7422..9167
/gene="Rsc9"
/locus_tag="P301_M10036"
/product="Rsc9p"
CDS 7422..9167
/gene="Rsc9"
/locus_tag="P301_M10036"
/note="Similar to YML127W 'Component of the RSC chromatin
remodeling complex; DNA-binding protein involved in the
synthesis of rRNA and in transcriptional repression and
activation of genes regulated by the Target of Rapamycin
(TOR) pathway'"
/codon_start=1
/product="Rsc9p"
/protein_id="EWG89195.1"
/translation="MNSLASNTPLNGTPVSEAPATSSEPVNMFETMVANPIKVSRLQS
NGVLTGPAANTKSIHYSLANFNVFQSLPKETARGVDDLTRMEMALLSGIPEEIKWSLK
KYLTYSNKAPYMISLRTLPDLLPLFKTFILPLERIVEGLNKSSICDSKAMDSLQMGLN
ALLILRNLAQDTDSVQILVKDREIKSFILFILKKFQCVATGDNKWQLYEGNATFFNEL
THYTLDLMEAISSYIAPAMKDDHYFQTLVSILNYTKDRYMVISILRSLSRLLVRSKAN
EESAADNLDHKTLSLIVSFLLLECDSELIIASLDFLYQYILPGSQRITELFKSKECSL
ILEATLPNLLSYNIATPDYHLLQKHKIRLIKRLKPPAPKEPPNLSEDLFQQLFKLNEP
LRSTAWLRCCFEPVQEAEFTQISLWRSYESKFGQPVRESGRKLLPAVEFIKNVSNAFN
NAAAIVITDPVTGKKRFVIKGIQPRFKALAIADGERESQVPISALKSKFLNDSKEITP
ARQNSIPEVKFPQELSDVSKVACTFLCLLSNDTDDGAGSAFCQRIRPLVLHKLADIPP
LTLALSEYMENTSGL"
gene complement(9414..10889)
/gene="Erg13"
/locus_tag="P301_M10041"
mRNA complement(9414..10889)
/gene="Erg13"
/locus_tag="P301_M10041"
/product="Erg13p"
CDS complement(9414..10889)
/gene="Erg13"
/locus_tag="P301_M10041"
/note="Similar to YML126C '3-hydroxy-3-methylglutaryl-CoA
(HMG-CoA) synthase, catalyzes the formation of HMG-CoA
from acetyl-CoA and acetoacetyl-CoA; involved in the
second step in mevalonate biosynthesis'"
/codon_start=1
/product="Erg13p"
/protein_id="EWG89196.1"
/translation="MKLSTKLCWCGIKGRLRPQKQQQLHNTNLQMTELKKQKTAEQKT
RPQNVGIKGMQIYIPTQCVNQSELEKFDGVSQGKYTIGLGQTNMSFVNDREDIYSMSL
TVLSKLIKSYNIDTNKIGRLEVGTETLIDKSKSVKSVLMQLFGENTDVEGIDTLNACY
GGTNALFNSLNWIESNAWDGRDAIVVCGDIAIYDKGAARPTGGAGTVAMWIGPDAPIV
FDSVRASYMEHAYDFYKPDFTSEYPYVDGHFSLTCYVKALDQVYKSYSKKAISKGLVS
DPAGSDALNVLKYFDYNVFHVPTCKLVTKSYGRLLYNDFRANPQLFPEVDAELATRDY
DESLTDKNIEKTFVNVAKPFHKERVAQSLIVPTNTGNMYTASVYAAFASLLNYVGSDD
LQGKRVGLFSYGSGLAASLYSCKIVGDVQHIIKELDITNKLAKRITETPKDYEAAIEL
RENAHLKKNFKPQGSIEHLQSGVYYLTNIDDKFRRSYDVKK"
gene complement(11115..12053)
/gene="Pga3"
/locus_tag="P301_M10046"
mRNA complement(11115..12053)
/gene="Pga3"
/locus_tag="P301_M10046"
/product="Pga3p"
CDS complement(11115..12053)
/gene="Pga3"
/locus_tag="P301_M10046"
/note="Similar to YML125C 'Putative cytochrome b5
reductase, localized to the plasma membrane; may be
involved in regulation of lifespan; required for
maturation of Gas1p and Pho8p, proposed to be involved in
protein trafficking'"
/codon_start=1
/product="Pga3p"
/protein_id="EWG89197.1"
/translation="MSKEDIEGTNILDEPVHGIYIPAALFVVGVAITTYMSGELKILW
SLPILFMIIFVRAYSAYKRRRSLYPDRWTSLELEDQTIISKNTALYRFKLKTRLESLD
IPAGHHVAVRVPIDGKQEVRYYNPISSKLESGYLDLVVKAYVDGKVSKYFAGLNSGDT
VDFKGPIGTLNYEPNSSKHLGIVAGGSGITPVLQILNEIITVPEDLTKVSLLYANETE
NDILLKDELDEMAEKYPHFQVHYVVHYPSDRWTGDVGYITKDQMNRYLPEYSEDNRLL
ICGPDGMNNLALQYAKELGWKVNSTRSSGDDQVFVF"
assembly_gap 12234..12294
/estimated_length=61
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(12404..14038)
/gene="Tub3"
/locus_tag="P301_M10056"
mRNA complement(join(12404..13716,14014..14038))
/gene="Tub3"
/locus_tag="P301_M10056"
/product="Tub3p"
CDS complement(join(12404..13716,14014..14038))
/gene="Tub3"
/locus_tag="P301_M10056"
/note="Similar to YML124C 'Alpha-tubulin; associates with
beta-tubulin (Tub2p) to form tubulin dimer, which
polymerizes to form microtubules; expressed at lower level
than Tub1p'"
/codon_start=1
/product="Tub3p"
/protein_id="EWG89198.1"
/translation="MREVISINVGQAGCQIGNACWELYSLEHGIKEDGHLEDGLSKPK
GGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELFHPEQLINGKEDAAN
NYARGHYTVGREIVDEVEERIRKMADQCDGLQGFLFTHSLGGGTGSGLGSLLLENLSY
EYGKKSKLEFAVYPAPQLSTSVVEPYNTVLTTHTTLEHADCTFMVDNEAIYDICKRNL
GISRPSFSNLNGLIAQVISSVTASLRFDGSLNVDLNEFQTNLVPYPRIHFPLVSYAPI
LSKKRATHESNSVSEITNACFEPGNQMVKCDPTKGKYMANCLLYRGDVVTRDVQRAVE
QVKNKKTVQMVDWCPTGFKIGICYEPPSVIPSSELANVDRAVCMLSNTTAIADAWKRI
DQKFDLMYAKRAFVHWYVGEGMEEGEFTEAREDLAALERDYIEVGADSYAEEF"
gene complement(14393..16156)
/gene="Pho84"
/locus_tag="P301_M10066"
mRNA complement(14393..16156)
/gene="Pho84"
/locus_tag="P301_M10066"
/product="Pho84p"
CDS complement(14393..16156)
/gene="Pho84"
/locus_tag="P301_M10066"
/note="Similar to YML123C 'High-affinity inorganic
phosphate (Pi) transporter and low-affinity manganese
transporter; regulated by Pho4p and Spt7p; mutation
confers resistance to arsenate; exit from the ER during
maturation requires Pho86p'"
/codon_start=1
/product="Pho84p"
/protein_id="EWG89199.1"
/translation="MSSVNKDTIHVAERSLHKEHLTEGGNMAFHNHLNDFAHIEDPLE
RRRLALESIDNEGFGWQQVKTISIAGVGFLTDSYDIFAINLGITMMSYVYWHGSMPGP
SQTLLKVSTSVGTVIGQFGFGTLADIVGRKRIYGMELIIMIVCTILQTTVAHSPAINF
VAVLTFYRIVMGIGIGGDYPLSSIITSEFATTKWRGAIMGAVFANQAWGQISGGIIAL
ILVAAYKGELEYANSGAECDARCQKACDQMWRILIGLGTVPGLACLYFRLTIPESPRY
QLDVNAKLELAAAAQEQDGEKKIHDTSDEDMAINGLERASTAVESLDNHPPKASFKDF
CRHFGQWKYGKILLGTAGSWFTLDVAFYGLSLNSAVILQTIGYAGSKNVYKKLYDTAV
GNLILICAGSLPGYWVSVFTVDIIGRKPIQLAGFIILTALFCVIGFAYHKLGDHGLLA
LYVICQFFQNFGPNTTTFIVPGECFPTRYRSTAHGISAASGKVGAIIAQTALGTLIDH
NCARDGKPTNCWLPHVMEIFALFMLLGIFTTLLIPETKRKTLEEINELYHDEIDPATL
NFRNKNNDIESSSPSQLQHEA"
gene 17241..18173
/gene="Gtr1"
/locus_tag="P301_M10076"
mRNA 17241..18173
/gene="Gtr1"
/locus_tag="P301_M10076"
/product="Gtr1p"
CDS 17241..18173
/gene="Gtr1"
/locus_tag="P301_M10076"
/note="Similar to YML121W 'Cytoplasmic GTP binding protein
and negative regulator of the Ran/Tc4 GTPase cycle;
component of GSE complex, which is required for sorting of
Gap1p; involved in phosphate transport and telomeric
silencing; similar to human RagA and RagB'"
/codon_start=1
/product="Gtr1p"
/protein_id="EWG89200.1"
/translation="MSSNNRKKLLLMGRSGSGKSSMRSIIFSNYSAFDTRRLGATIDV
EHSHLRFLGNMTLNLWDCGGQDVFMENYFTKQKDHIFQMVQVLIHVFDVESTEVLKDI
EIFAKALKQLRKYSPDAKIFVLLHKMDLVQLDKREELFQIMMKNLSETSSEFGFPNLI
GFPTSIWDESLYKAWSQIVCSLIPNMSNHQSNLKKFKEIMNALEIILFERTTFLVICS
SNGENSNENHDSSDNNNVLLDPKRFEKISNIMKNFKQSCTKLKSGFKTLILNNNIYVS
ELSSNMVCFIVLKDMNIPQELVLENIKKAKEFFQ"
gene complement(18575..20116)
/gene="Ndi1"
/locus_tag="P301_M10081"
mRNA complement(18575..20116)
/gene="Ndi1"
/locus_tag="P301_M10081"
/product="Ndi1p"
CDS complement(18575..20116)
/gene="Ndi1"
/locus_tag="P301_M10081"
/note="Similar to YML120C 'NADH:ubiquinone oxidoreductase,
transfers electrons from NADH to ubiquinone in the
respiratory chain but does not pump protons, in contrast
to the higher eukaryotic multisubunit respiratory complex
I; phosphorylated; homolog of human AMID'"
/codon_start=1
/product="Ndi1p"
/protein_id="EWG89201.1"
/translation="MLSKNLYSNKRLLTSTNTLVRFASTRSTGVENSGAGPTSFKTMK
VIDPQHSDKPNVLILGSGWGAISFLKHIDTKKYNVSIISPRSYFLFTPLLPSAPVGTV
DEKSIIEPIVNFALKKKGNVTYYEAEATSINPDRNTVTIKSLSAVSQLYQPENHLGLH
QAEPAEIKYDYLISAVGAEPNTFGIPGVTDYGHFLKEIPNSLEIRRTFAANLEKANLL
PKGDPERRRLLSIVVVGGGPTGVEAAGELQDYVHQDLRKFLPALAEEVQIHLVEALPI
VLNMFEKKLSSYAQSHLENTSIKVHLRTAVAKVEEKQLLAKTKHEDGKITEETIPYGT
LIWATGNKARPVITDLFKKIPEQNSSKRGLAVNDFLQVKGSNNIFAIGDNAFAGLPPT
AQVAHQEAEYLAKNFDKMAQIPNFQKNLSSRKDKIDLWFEENNFKPFKYNDLGALAYL
GSERAIATIRSGKRTFYTGGGLMTFYLWRILYLSMILSARSRLKVFFDWIKLAFFKRD
FFKGL"
gene 20920..21993
/locus_tag="P301_M10101"
mRNA 20920..21993
/locus_tag="P301_M10101"
/product="hypothetical protein"
CDS 20920..21993
/locus_tag="P301_M10101"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89202.1"
/translation="MSPSPSVSPRRTLNNKSSYINNSGGLVLPPTQFNLNQQPVLSFQ
QKATFDSNQQFFYYPESPTKNLRPRFNSVSQVNKGVNEDHYTGGGSSNNNRPSRYTNT
MGAANTNVNSHPHHQSVSHLNSKSLKFNQTKEVSSINEIIFPSRTCTKKRYFTKPIDL
YGTRSSTSVAPKLTNSPTKSKTNFNIKKCILPRSVVTTYKLPSPVHETIDDISKKIII
LLISLKFEKNYHFLQPIQLSTNSKTRISKSLDELCGVQLTSTLRQQKQLQGNSKPVKN
LPNSNAKQRAGASVSTNANESFELSFDGKAMDRSDIFRMVDSFSIAISDEDEEDEEED
SFQQRSANNRILPAEILSNEPLK"
gene 22643..24151
/gene="Ngl3"
/locus_tag="P301_M10121"
mRNA 22643..24151
/gene="Ngl3"
/locus_tag="P301_M10121"
/product="Ngl3p"
CDS 22643..24151
/gene="Ngl3"
/locus_tag="P301_M10121"
/note="Similar to YML118W 'Putative endonuclease, has a
domain similar to a magnesium-dependent endonuclease motif
in mRNA deadenylase Ccr4p; similar to Ngl1p and Ngl2p'"
/codon_start=1
/product="Ngl3p"
/protein_id="EWG89203.1"
/translation="MDSQVEGKISPSQKESSSTSGLVSPSEDGPAHQKIHRDQLSLDQ
IKKIREERAQKRQVRRNSLISQGKDPDFPTPDLQFIERPFLPINHDNSKGLTPATIQV
TQDSLDVKIMTYNTLAQTLIRRDFFPESGPALKWHKRSKVLVHELKKYRPDVVSLQEV
DYNELNFWQENFHKLGFDMIFKRHEGKTHGLLVAWNNKKFQLDNDWMLDYDNILAGNV
ISARTRTKNIALIISLYFKGITDSSSRGIIVANTHLFWHPFGVFERLRQSYLVLQKIQ
EIKACSKYNGWHSLLMGDFNTEPEEPPYLAITKRPLILKGPIRAMVECSLAYRYSKKR
NGEESDQDDEECDEKSRGEGHSDQPQNPKPESFTATKEEKALVNQLVALHNSLHVKGV
SLYGIGYGKVHPENANGSHGEPGLSNWANTWCGLLDYIFYIEGDHNQDTRQKEPLNAF
EGNNNVKIIGYLRMPCAQEMPKHSQPFEGEYASDHISLMCQIRLFFGGGKST"
gene 24553..27957
/gene="Nab6"
/locus_tag="P301_M10131"
mRNA 24553..27957
/gene="Nab6"
/locus_tag="P301_M10131"
/product="Nab6p"
CDS 24553..27957
/gene="Nab6"
/locus_tag="P301_M10131"
/note="Similar to YML117W 'Putative RNA-binding protein
that associates with mRNAs encoding cell wall proteins in
high-throughput studies; deletion mutants display
increased sensitivity to some cell wall disrupting agents;
expression negatively regulated by cAMP'"
/codon_start=1
/product="Nab6p"
/protein_id="EWG89204.1"
/translation="MSNSNSKKPVANYAYRQQQDYNGMNAMVGNPMMYHPVDFVNGAG
QYGPSQHPAYYTNSPLPNIPPTPFDTAYGASLLPSHLLMGSPFVSSPNMQSGYNSARS
SNLKRKAYSRPVSNHNGYNGNSNSNQNNTNNGMVTPSNYYRVGRNSFPRNNNSTRNVT
HNNNKGCDTRNNSGRRTFTRNNIFDDINPEMLLQRPFCINYKVLPTGDDAYRTRSLLI
ENVDHSIDLHSIVKNFVKSNTLESAYLIEGGKSDDSKDVETKNLSILISFLTKGDCLN
FYNNILQRLSEFKTFLKSEALNLKFVCLNYDPKCLPTFIESEALTENAEEADITNGST
MISASLHHNIANKDATRSIIIEFKSPVEKSDLFKKKLQFLDRSKNKRYILESIDLVNT
DVPSNQFPENYAVLTFLNISMAIEVLDYLKKYSKNLGISKCFYVSLAPLVVSSARSSV
ANIYEGKTSTHRLSVPSVTAGNNNDSNNNGNNNKSNMSGITTLNNNSSIGVSVYGHSN
MSLTSLSSSVSLNEEIDMLATKLQGVELDGTYLEINYRDYQTPTIEEHSTHLSNVKIS
KTTENSRQFSQDIPSPLPLNEHMFMNDSNQSNGAIIPQQLIATPSPVSPNLQMNQRVL
PNPITQSLEQNFNVSAKVASSMGSDIGNRTIYIGNINPRSKAEDICNVVRGGILQSIK
YIPEKKICFVTFIEAPSAVQFYANSFIDPIVLHGNMLRVGWGHYSGPLPKSISLAVTI
GASRNVYVSLPEFAFKEKFIHDPQYKKLHETLSLPDAEQLREDFSTYGDIEQINYLSD
SHCCWINFMNISSAISLVEEMNKESTVQNESGEVTLKRATEEKFGGRYKGLLINYGKD
RCGNINKNLIAGKNSRFYKKVKRPSYNIRLSKLEEKRRQNEIDEKEKAFDKPLNLESL
GISLDAHKDNGGGETGTANNTGHENESELEAENENGNETGSFGGLGLAVASSDVKRAT
SDETDYEDIFNKSSGSSDSSSDVEVIMHSPSDPEYALKSQTLRSSSQTVINSKRPVKI
EDEEEAVGMSQLNYRSSLRQAPPRAPSTLSYNHSKNNETPMQDILTNGETANNRKKKR
GSFPRHRTIPGSDVMAQYLAQVQHSTFMYAANILGASAEDNTHPDE"
gene 27782..28084
/locus_tag="P301_M10136"
mRNA 27782..28084
/locus_tag="P301_M10136"
/product="hypothetical protein"
CDS 27782..28084
/locus_tag="P301_M10136"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89205.1"
/translation="MAKQQITERRREDLFQGIERYQDLTSWPNTLHKCNIRHLCMQPI
FWVPLRKTTRILTSNIAVLSLKYNAYPSDYLDKAQGSLRRPAKVFLPFFLRFLCIN"
gene 28553..30181
/gene="Atr1"
/locus_tag="P301_M10141"
mRNA 28553..30181
/gene="Atr1"
/locus_tag="P301_M10141"
/product="Atr1p"
CDS 28553..30181
/gene="Atr1"
/locus_tag="P301_M10141"
/note="Similar to YML116W Multidrug efflux pump of the
major facilitator superfamily, required for resistance to
aminotriazole and 4-nitroquinoline-N-oxide"
/codon_start=1
/product="Atr1p"
/protein_id="EWG89206.1"
/translation="MGNQSLVVLTESKGEYENETELTVKKSSRDNNIGESLTATAFTQ
SEDEMVDSNQKWQNPNYFKYAWQEYLFIFTCMISQLLNQAGTTQTLSIMNILSDSFGS
EGNSKSWLMASFPLVSGSFILISGRLGDIYGLKKMLLVGYVLVIIWSLICGITKYSGS
DTFFIISRAFQGLGIAFVLPNVLGIIGNIYVGGTFRKNIVISFVGAMAPIGATLGCLF
AGLIGTEDPKQWPWAFYAYSIAAFINFVLSIYAIPSTIPTNIHHFSMDWIGSVLGVIG
LILLNFVWNQAPISGWNQAYIIVILIISVIFLVVFIIYEIRFAKTPLLPRAVIKDRHM
IQIMLALFFGWGSFGIFTFYYFQFQLNIRQYTALWAGGTYFMFLIWGIIAALLVGFTI
KNVSPSVFLFFSMVAFNVGSIMASVTPVHETYFRTQLGTMIILSFGMDLSFPASSIIF
SDNLPMEYQGMAGSLVNTVVNYSMSLCLGMGATVETQVNSDGKHLLKGYRGAQYLGIG
LASLACMISGLYMVESFIKGRRARAAAEYDCTVA"
assembly_gap 30367..31420
/estimated_length=1054
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<31421..32876)
/gene="Van1"
/locus_tag="P301_M10151"
mRNA complement(<31421..32876)
/gene="Van1"
/locus_tag="P301_M10151"
/product="Van1p"
CDS complement(<31421..32876)
/gene="Van1"
/locus_tag="P301_M10151"
/note="Similar to YML115C 'Component of the mannan
polymerase I, which contains Van1p and Mnn9p and is
involved in the first steps of mannan synthesis; mutants
are vanadate-resistant'"
/codon_start=1
/product="Van1p"
/protein_id="EWG89576.1"
/translation="MGMFFNLRSNIKKKAMDNGLSLPISRNGSSNNIKDKRSEHNSNS
LKGKYRYQPRSTPSKFQLTVSITSLIIIAVLSLYLFISFLSGMGIGVSTQNGRSLLGS
SKSSENYKTIDLEDEEYYDYDFEDIDPEVISKFDDGVQHYLISQFGSEVLTPKDDEKY
QRELNMLFDSTVEEYDLSNFEGAPNGLETRDHILLCIPLRNAADVLPLMFKHLMNLTY
PHELIDLAFLVSDCSEGDTTLDALIAYSRQLQNGTLSQIFQEIDAVIDSQTKGTDKLY
LKYMDEGYINRVHQAFSPPFHENYDKPFRSVQIFQKDFGQVIGQGFSDRHAVKVQGIR
RKLMGRARNWLTANALKPYHSWVYWRDADVELCPGSVIQDLMSKNYDVIVPNVWRPLP
TFLGTEQPYDLNSWMESQEALALAKTLDEDDVIVEGYAEYPTWRVHLAYIRDAEGDPN
EAVDLNGVGGVSILAKAKIFRNGVQFPAFTFENHA"
gene complement(33119..34651)
/gene="Taf8"
/locus_tag="P301_M10161"
mRNA complement(33119..34651)
/gene="Taf8"
/locus_tag="P301_M10161"
/product="Taf8p"
CDS complement(33119..34651)
/gene="Taf8"
/locus_tag="P301_M10161"
/note="Similar to YML114C TFIID subunit (65 kDa), involved
in RNA polymerase II transcription initiation"
/codon_start=1
/product="Taf8p"
/protein_id="EWG89207.1"
/translation="MTSKTSESGTGTQSTIVQLRNLPDLTEISHLEIDAPVVEILKKT
VLFQLNSLNICISNFALDELVNLVTVQMDGMFRNLHNLTLLQRRSQASQADLKLLLRE
FNLDAPSLYQQFQASEFIKSKHSTEYEKLMSWSSLAALPHNEEDEEDELNNIEEQQNE
INVLLPPSNPLEKQIPSWLPNFPPDHTYKFTPEFNHPITDLKTIKKEIVKESQESEKA
LLNLNKSLSHISSASNTPQPPGLDDEDAIEQQLEIWGNALEERKPTITEKSFNENNIE
QYAKYRVELARERVTKFEVNQLKRTKNPFLKISETLYLPESPHQSHKTIQKTIELQFR
KSMTLFMHNLPKVQKLKKEKIRMAKEERAKSLKRRQEELISQRTKREQDEGHDLELLL
NNEHARDAADDTTTPNALNNSTIVINTNAEDEDDDINLFGILGSSEDENEMSSMPAEN
LVAESEPPTITAQDTTNTTPVAHNTTNIDATTSHSPHSTPNENAPTSPPADIATDHDI
TM"
gene 34862..36002
/gene="Dat1"
/locus_tag="P301_M10166"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 34949..35175
/estimated_length=227
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 36274..37164
/gene="Ctk3"
/locus_tag="P301_M10171"
mRNA 36274..37164
/gene="Ctk3"
/locus_tag="P301_M10171"
/product="Ctk3p"
CDS 36274..37164
/gene="Ctk3"
/locus_tag="P301_M10171"
/note="Similar to YML112W Gamma subunit of C-terminal
domain kinase I (CTDK-I), which phosphorylates both RNA
pol II subunit Rpo21p to affect transcription and pre-mRNA
3' end processing, and ribosomal protein Rps2p to increase
translational fidelity"
/codon_start=1
/product="Ctk3p"
/protein_id="EWG89208.1"
/translation="MDSLEARLQFIQVLKNLQKTLHKTRDSITSSSTTTPPSSQQKLN
NDPIQFYLRNYRHHYEDFHQCLFDTTMKMDPLDRLDVVIYYVRIIRNLYPHSHSNTNV
TKVLNEVLLMDIDLVFELCLPCQDWKSLTNQATCKELFLDLSKLIHYDATSVTHTPSD
TTLIDATTWYSVKTERTTKDYKESLQRTESLLKDRDLKKLAFFQQFNSDTTAINPDLQ
TQPTNANILLHRMEADRELHKRSKETSWYIERPSNDILDESEFKSLWTHFETTDSGFD
KDDYKNIKALNDIAKASYMY"
gene 38159..40921
/gene="Bul2"
/locus_tag="P301_M10181"
mRNA 38159..40921
/gene="Bul2"
/locus_tag="P301_M10181"
/product="Bul2p"
CDS 38159..40921
/gene="Bul2"
/locus_tag="P301_M10181"
/note="Similar to YML111W Component of the Rsp5p
E3-ubiquitin ligase complex, involved in intracellular
amino acid permease sorting, functions in heat shock
element mediated gene expression, essential for growth in
stress conditions, functional homolog of BUL1"
/codon_start=1
/product="Bul2p"
/protein_id="EWG89209.1"
/translation="MTFTFSTSSRKNGRPPLKSVSTEDNIHLLRKRRQQQLSSNSTDN
SLHPNSGQTPRASDSQDDDIRSASTTNLDRLRQEREENSLEMDCTQSRLSHRANMLVD
VLPSFEMYNALHRHIPQGNVDPDRHDFPPSYQEVRTQRMTILPSNDNSVERSQLTAVP
GSENACNNATAHSLTNLHPLQTQHLTINSTRSGGQSLHSSSDTNISQIAFEDDLNDSD
NIFINKLYTLPKLSTPIEIDIRITKTASIPHERPEEQSILKEYTSGDIIHGYCLIENR
SSQPLKFEMFYVTLEAYISVIDRQKGKRTLKRFLRMVDLSASWSYTNITPSTGINIVP
GERDFDDAIIGLSNSRELKPNTKYKKFFMFKLPTQLLDVTCKQEQFSHCLLPPSFGID
KYKNNCKYSGIKVNSVLGCGHLGTKGSPILTLDMADDNLSINYTIDAKIVGKDKRTSK
LNIMKEKEYNLRVMPFPFAGVTNQQNEKTCLRQLKNLESLIEDRFEALNKIFKKLELN
EAISNVDIHDTDISGTLDGNEDLDSDEILRRKLDQLHINNRIDDTASQSPSYDSKNMA
PKENLVETELRYKFKNKNKSNSSLFSHFLSSSETGSSSTGPHVYNSGLIVLSVKKPQS
ALPYWSPSLLRKTNKFEAKSEQEKENWQRLMGMLPEGVKTPLTKLDVHLTCIQSNNSA
GHKPPEISSVTTEFVVITAKSDNSIPIKFCTELLMNENRLNKLKTKFLTYQKKVHEYR
KKFEENHAKLNELYNRNRDHFTPKELLFTNFISDQINNDIDSLAGLKVNIIDLHDIFK
KQIHTFEEENEDIISKKGSSNPPSASSSNNNFLQATFSNGASTATKFTQQIVHEWEKV
KPLQYKRDVTVNLKLNPNIKETLVPNFETCLCCRFYCVRVNIKFDNHLGSMKVDIPVD
VKKLQI"
assembly_gap 40937..41159
/estimated_length=223
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(41253..42176)
/gene="Coq5"
/locus_tag="P301_M10186"
mRNA complement(41253..42176)
/gene="Coq5"
/locus_tag="P301_M10186"
/product="Coq5p"
CDS complement(41253..42176)
/gene="Coq5"
/locus_tag="P301_M10186"
/note="Similar to YML110C
'2-hexaprenyl-6-methoxy-1,4-benzoquinone
methyltransferase, involved in ubiquinone (Coenzyme Q)
biosynthesis; localizes to the matrix face of the
mitochondrial inner membrane in a large complex with other
ubiquinone biosynthetic enzymes'"
/codon_start=1
/product="Coq5p"
/protein_id="EWG89210.1"
/translation="MLISSRIVRSSLVNVPLRLSRFFTQAHRACKEEEVNSPLSSTAE
QPEQKYTHFGSKTVLKSTKQKLVGDVFSSVANRYDLMNDVMSLGIHRLWKDHFINKLD
AGKRPNSTTPLNFIDVAGGSGDIAFGLLDHAESKFGDTESTMDIVDINPDMLKEGEKR
AMEQGKYFKDPRVRFLVSNGEKLEEIDSDSKDIYTVSFGIRNFTDIQKGLNTAYRVLK
PGGIFYCLEFSKIENPLMDFAYQQWAKVLPVMGSMIANDYDSYQYLVESIERFPDQET
FKSMIEKAGFKSAGYESLTFGICAIHWGIKV"
gene 42862..45693
/gene="Zds2"
/locus_tag="P301_M10191"
mRNA 42862..45693
/gene="Zds2"
/locus_tag="P301_M10191"
/product="Zds2p"
CDS 42862..45693
/gene="Zds2"
/locus_tag="P301_M10191"
/note="Similar to YML109W 'Protein with a role in
regulating Swe1p-dependent polarized growth; interacts
with Cdc55p; interacts with silencing proteins at the
telomere; implicated in the mitotic exit network through
regulation of Cdc14p localization; paralog of Zds1p'"
/codon_start=1
/product="Zds2p"
/protein_id="EWG89211.1"
/translation="MVLMEDVQNKDGHNTVENSSGGTDSNNNIQMRRMRKTQLSKKEL
FEKRKSDVLIAAKSLDTEIQNVKNLKRLSIGSMDLVIDPELEFKVNSRNSYSSDSSKE
SLQESLHEENIIRSEQKEEQGSEDNDAYEEGDATNVDDSIDITQTEYLHDEETLEKEK
IIRNASSSTSSSARVTSRNRRLSGVKTLAHDVVLDVENDHDSKMVDLTQNLLWVPADQ
HPNVKPENYLELIQDTLQNIQISTNQDIDENKLELGNNHVISNRKRTGSVVRRPSRLK
TSYTKFDDEPPLADKPQEGEIQVDKRISSSDIKTIRSVSLKEITEELTKISNNAGLTD
SDAVTLARSLSMSGSFTNESLHLNGNHTENDNEFASNMFNETGLTIPERSSLRRSKFN
TYKIRLEGSSLPQAVKLNSLMNIQTNDNRRSASSPASYTQVPQEQASLNDFHEIFDHY
RRTSTDWSTENEKYVDSTNYYSDEEDLTHASISQESSLLSTDSSNNSVLIKPHNTGSM
ISEKLDQHVSSSEKSNTNNSEPNHGWSWLNSSNGSLNGNEQTYQQLTDDEDDEECVDN
EKADFVNLSVSRRAKSTKRASERINHSKNRHSPIFQIHSEEAKSVVITPSVVSSSESQ
PSKPTAPAAVEKKVELPTDTQASTHKKNSLEKRLAKLFKRKQHSGTCKSDVKVIKKSV
KKELKKKASHSSLSKFRKSPKKKPQEAEVNCERPSSPTKTITEDIDTESVIEPEVRSS
NASTLLPDSHTSHSSEFVVETISELDGDDSFDISGGDVNYDVEVHSSISRDTTAGLEE
DIGAEREDNTSPTAPQISTLPPRKLTFEDVVKPDYPNAPIKFTDSAFGFPLPMITNST
VIMFDHRLGINVERAIYRLSHLKLSDPGRELRQQVLLSNFMYSYLNLVNHTLYMEQVG
TGDIAFNGDSALGMMDKNDSDGTILIPDI"
gene 46018..46335
/locus_tag="P301_M10196"
mRNA 46018..46335
/locus_tag="P301_M10196"
/product="hypothetical protein"
CDS 46018..46335
/locus_tag="P301_M10196"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89212.1"
/translation="MSKSNTYRMLVLLEDDTKINKEDEKFLKGKPGKMHEFVDELVLP
FNVDELDELNTWFDKFDAEICIPNEGHIKYEISSDGLIVLMLDKEIEEVVEKVKKFVD
ENN"
gene complement(46490..47494)
/gene="Pml39"
/locus_tag="P301_M10201"
mRNA complement(46490..47494)
/gene="Pml39"
/locus_tag="P301_M10201"
/product="Pml39p"
CDS complement(46490..47494)
/gene="Pml39"
/locus_tag="P301_M10201"
/note="Similar to YML107C 'Protein required for nuclear
retention of unspliced pre-mRNAs along with Mlp1p and
Pml1p; anchored to nuclear pore complex via Mlp1p and
Mlp2p; found with the subset of nuclear pores farthest
from the nucleolus; may interact with ribosomes'"
/codon_start=1
/product="Pml39p"
/protein_id="EWG89213.1"
/translation="MEKDALEVRLKSIRHSLDNNTKLLPGKYRNTLGERLITKWRYKK
KSHNGSSMLPEKCKSHVQLYDDLVQESSKHFVGFRLHDLRALLKRICSIQNYTRHVLI
EWDVRWVNPLTLASKGWEPYQSASQSQVPFKCCCCHAIMTIPLLKNGDDVADYNMKLN
EKIWNCNIIGNHLQKCPWRENQVDLNKEYYLSSQNLIREIERIHTEIDRIVSGSNEFS
LKRNSSRIFHYLSEKEIQKLAFFFDCKDYSLVGLLLLGYTKFQKDDLVQCTACFHRAS
LKKLEYTEFNGHALWCRYYNKELLPTMLLELIGKEDKLITKLGVGERLNKLEAVLQTL
"
gene 47998..48678
/gene="Ura5"
/locus_tag="P301_M10211"
mRNA 47998..48678
/gene="Ura5"
/locus_tag="P301_M10211"
/product="Ura5p"
CDS 47998..48678
/gene="Ura5"
/locus_tag="P301_M10211"
/note="Similar to YML106W 'Major orotate
phosphoribosyltransferase (OPRTase) isozyme that catalyzes
the fifth enzymatic step in de novo biosynthesis of
pyrimidines, converting orotate into
orotidine-5'-phosphate; minor OPRTase encoded by URA10'"
/codon_start=1
/product="Ura5p"
/protein_id="EWG89214.1"
/translation="MPIMLEDYQKNFLELAIECQALRFGSFKLKSGRESPYFFNLGLF
NTGKLLSNLATAYAIAIIQSDLKFDVIFGPAYKGIPLAAIVCVKLAEIGGSKFQNIQY
AFNRKEAKDHGEGGIIVGSALENKRILIIDDVMTAGTAINEAFEIISNAKGQVVGSII
ALDRQEVVSTDDKEGLSATQTVSKKYGIPVLSIVSLIHIITYLEGRITAEEKSKIEQY
LQTYGASA"
gene complement(49091..49912)
/gene="Sec65"
/locus_tag="P301_M10216"
mRNA complement(49091..49912)
/gene="Sec65"
/locus_tag="P301_M10216"
/product="Sec65p"
CDS complement(49091..49912)
/gene="Sec65"
/locus_tag="P301_M10216"
/note="Similar to YML105C 'Subunit of the signal
recognition particle (SRP), involved in protein targeting
to the ER; interacts with Srp54p; homolog of mammalian
SRP19'"
/codon_start=1
/product="Sec65p"
/protein_id="EWG89215.1"
/translation="MPRLEEIDDFNDIDDLDMELAELDPSLRTPIAPKITPKVVRSQD
QENPAFLPGTNNNSNSNNNSSNEKEQLSFINPKTGKVERSEAISKKDLEEVKRFQVLY
PCYFDINRSHKEGRRVPKELAVENPLAKTMADAVRELGILCIFEGEKCHPQDFGNPGR
IRVLFKENGQLIGAATKFKGGKRQLMKAVGEYMKRHPTTIESLREIPYGPDFDNIEFK
KIPRVKGFKMNEIVPLHSPFLMGHPMTKSVYETPKITAAEKSFKPPKNKYKVVRR"
gene complement(50161..53544)
/gene="Mdm1"
/locus_tag="P301_M10221"
mRNA complement(50161..53544)
/gene="Mdm1"
/locus_tag="P301_M10221"
/product="Mdm1p"
CDS complement(50161..53544)
/gene="Mdm1"
/locus_tag="P301_M10221"
/note="Similar to YML104C 'Intermediate filament protein,
required for nuclear and mitochondrial transmission to
daughter buds; contains a Phox homology (PX) domain and
specifically binds phosphatidylinositol 3-phosphate
(PtdIns-3-P)'"
/codon_start=1
/product="Mdm1p"
/protein_id="EWG89216.1"
/translation="MPKFPQFRLILVLFYLISMIQWSVITFSLGFFLNVCIFAYFVFF
KSLPDLPKPQPRFVDIVPESSNTVDVDKELKSVEGLIQDGNAQIGKELESIVNLIIKD
FVEPWFTKIDKNSDAEFLKVIKWRLLQTLLVVKDKLMKNDSASLIVLKLLPIFNKHFS
TFCDAREAVLSDLTLERHKSANIDLQIAVEFNKNYKIHKSLSLKPNALQKEIEKSIRK
TVIGLLPHLFDNDELDSLLVFTLMTEVLTTCIISPLIFKFTDPDSWNLRIVSLSQNYF
EEKHKVHKIRRMLSKELQDHKKVMNDVANKDVGELSSEKLELNAEYTGKQFEHYLNQL
DSLLDLSDIKYVAYSLALKIYQLKENEHLTRENLKYKKRLLLSLNLIESKLSVPGSEI
DTASKKLAREANYPDLNMDNGIVLKEMASFLTSITLKDIVDDSEFLPFFESFLGSVPE
TQGSTFLEYSQTIESFKNPLEDATSEDIISGYSGISTMQLQEISSKFFHNNNLQNMKL
LDEGLVKNIILFRNSFQINNDEDTFILARKSVLLLQTEAIKYLDDRFLPLFKKTPSFL
KMLSTSHIISTDIYAHFLSRIGGVNNPEQNKIIKDNVKTDFMNPVRIFANPGITDALD
NIVNGSESKPDKSRISSNPRYSQLFGSENDNIFKDKLFDDENDNTSEISVVEDQLDHP
RNMEKVSVSSGNSGLNPSQFYGSNNFRDNIASLTISIDQIEKELELLRHLILKADLTN
NQMQLKILKKSQRTLLKELEMKELLKQQYMVQENGNSLFRKTKIYIRSYFSENSSNGL
KEITYYIINIHHFNNGQVSSWDMARRYNEFFELNTYLKKNFRDLMRQLQDLFPSKVKM
SLKYHVTKTLLYEERKQKLEKYLRELLSISEICEDNIFRRFLIDPTPFKLNKEYMHDD
ILEEPLHEPIGSSNSTSNSSSVVDLQSSEDGGELNFYEDERHFFTDSGYPFYSQNKSF
VKQICDLFISLFALNKANAGWLRGRAIITVLQQLLGSTIEKYIKVSIQKLRSEDQVFE
AIVTFKNMLWGDNGLFERKRNETAEATRSEGEKLRTEQLALTSLQRLFADTCGRVVGL
RDSHEAAGRVHAMLQNPYLNASLLLEALDAILLDIICND"
gene complement(53804..58764)
/gene="Nup188"
/locus_tag="P301_M10226"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 57482..57527
/estimated_length=46
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(58983..59153)
/locus_tag="P301_M10231"
ncRNA complement(58983..59153)
/ncRNA_class="snoRNA"
/locus_tag="P301_M10231"
/product="SNR85"
gene 59510..60916
/gene="Cac2"
/locus_tag="P301_M10236"
mRNA 59510..60916
/gene="Cac2"
/locus_tag="P301_M10236"
/product="Cac2p"
CDS 59510..60916
/gene="Cac2"
/locus_tag="P301_M10236"
/note="Similar to YML102W Component of the chromatin
assembly complex (with Rlf2p and Msi1p) that assembles
newly synthesized histones onto recently replicated DNA,
required for building functional kinetochores, conserved
from yeast to humans"
/codon_start=1
/product="Cac2p"
/protein_id="EWG89217.1"
/translation="MEASHLQIYWHDSQPVYSLTFQKNSANDKLFTAGGDNKVRIWKL
NRDENGQNGGVRKIESLDFLGSLTHHEQAINVIRFNSKGDVLASAGDDGQVLLWKQED
PNTQQESVVRPFGMDAETSEADENKEKWVVWKRLRGGSGATAAAEIYDLAWSPDNRNI
VVACMDNSIRLFDVGAGTLVCGQSDHGHYVQGVAWDPLNQFILSQSADRSLHVYGVIL
SSAGVVTGLKLRSKIAKAELPCPGDVLRTNYLFHNETLPSFFRRCSISPCGGLVVVPS
GVYKVAGDEVANCVYVYTRSGILNSAGGVKNRPAIRIPSLKKPALMAAFSPVFYETCQ
KSVLKLPYKLVFAIATTNEVLVYDTDVLEPLCVVGNIHYSPITDLAWSEDGSTLLISS
TDGFCSYVSIDTETQFGSRIEPPAMHAEPLDTDESAVAAKNQREAGGIVNMLPVKKIP
CNSSDSKKRRIHPTPVDL"
gene complement(60625..60942)
/locus_tag="P301_M10241"
mRNA complement(60625..60942)
/locus_tag="P301_M10241"
/product="hypothetical protein"
CDS complement(60625..60942)
/locus_tag="P301_M10241"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89218.1"
/translation="MYRIKYIKNHKSTGVGCMRLFLLSLLLQGIFFTGSMFTIPPASR
WFLAATALSSVSSGSACIAGGSILEPNCVSVSIDTYEQNPSVDEISRVEPSSDHAKSV
MGE"
gene complement(60951..61304)
/gene="Cue4"
/locus_tag="P301_M10246"
mRNA complement(60951..61304)
/gene="Cue4"
/locus_tag="P301_M10246"
/product="Cue4p"
CDS complement(60951..61304)
/gene="Cue4"
/locus_tag="P301_M10246"
/note="Similar to YML101C 'Protein of unknown function;
has a CUE domain that binds ubiquitin, which may
facilitate intramolecular monoubiquitination'"
/codon_start=1
/product="Cue4p"
/protein_id="EWG89219.1"
/translation="MDGSTIVFILTMVCLFVYTVKHRGAKQVPSRTVQDAKPAPSVAT
NDPSPEPVPSAPEERVARLNRHGSDRKRAVNSDMVEIVMTMAPHVPQEKVVQDLRNTG
SIEHTMENIFAGKLD"
gene 61354..61529
/locus_tag="P301_M10251"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 61842..65138
/gene="Tsl1"
/locus_tag="P301_M10256"
mRNA 61842..65138
/gene="Tsl1"
/locus_tag="P301_M10256"
/product="Tsl1p"
CDS 61842..65138
/gene="Tsl1"
/locus_tag="P301_M10256"
/note="Similar to YML100W 'Large subunit of trehalose
6-phosphate synthase (Tps1p)/phosphatase (Tps2p) complex,
which converts uridine-5'-diphosphoglucose and glucose
6-phosphate to trehalose, similar to Tps3p and may share
function; mutant has aneuploidy tolerance'"
/codon_start=1
/product="Tsl1p"
/protein_id="EWG89220.1"
/translation="MALIVASLFLPYQPQFELDTSLPENSQVDSSLVNIQAMANDQQQ
QRALSNNISQESLVAPAPEQGVPPAISRSATRSPSAFNRASSTTNTATLDDLVSSDIF
MENLTANATTSHTPTSKTMLKPRKNGSVERFFSPSSNIPTDRIASPIQHEHDSGSRIA
SPIQQQQQDPTANLLKNVNKSLLVHSLLNNTSQTSLEGPNNHIVTPKSRAGNRPTSAA
TSLVNRTKQGSASSGSSGSSAPPSIKRITPHLTASAAKQRPLLAKQPSNLKYSELADI
SSSETSSQHNESDPDDLTTAPDEEYVSDLEMDDAKQDYKVPKFGGYSNKSKLKKYALL
RSSQELFSRLPWSIVPSIKGNGAMKNAINTAVLENIIPHRHVKWVGTVGIPTDEIPEN
ILANISDSLKDKYDSYPVLTDDVTFKAAYKNYCKQILWPTLHYQIPDNPNSKAFEDHS
WKFYRNLNQRFADAIVKIYKKGDTIWIHDYHLMLVPQMVRDVLPFAKIGFTLHVSFPS
SEVFRCLAQREKILEGLTGADFVGFQTREYARHFLQTSNRLLMADVVHDEELKYNGRV
VSVRFTPVGIDAFDLQSQLKDGSVMQWRQLIRERWQGKKLIVCRDQFDRIRGIHKKLL
AYEKFLVENPEYVEKSTLIQICIGSSKDVELERQIMIVVDRINSLSTNISISQPVVFL
HQDLDFSQYLALSSEADLFVVSSLREGMNLTCHEFIVCSEDKNAPLLLSEFTGSASLL
NDGAIIINPWDTKNFSQAILKGLEMPFDKRRPQWKKLMKDIINNDSTNWIKTSLQDIH
ISWQFNQEGSKIFKLNTKTLMEDYQSSKKRMFVFNIAEPPSSRMISILNDMTSKGNIV
YIMNSFPKPILENLYSRVQNIGLIAENGAYVSLNGVWYNIVDQVDWRNDVAKILEDKV
ERLPGSYYKINESMIKFHTENAEDQDRVASVIGDAITHINTVFDHRGIHAYVYKNVVS
VQQVGLSLSAAQFLFRFYNSASDPLDTSSGQITNIQTPSQQNPSDQEQQPPASPTVSM
NHIDFACVSGSSSPVLEPLFKLVNDEASEGQVKAGHAIVYGDATSTYAKEHVNGLNEL
FTIISRIIED"
gene 65446..65775
/locus_tag="P301_M10261"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(65615..68257)
/gene="Arg81"
/locus_tag="P301_M10266"
mRNA complement(65615..68257)
/gene="Arg81"
/locus_tag="P301_M10266"
/product="Arg81p"
CDS complement(65615..68257)
/gene="Arg81"
/locus_tag="P301_M10266"
/note="Similar to YML099C 'Zinc-finger transcription
factor of the Zn(2)-Cys(6) binuclear cluster domain type,
involved in the regulation of arginine-responsive genes;
acts with Arg80p and Arg82p'"
/codon_start=1
/product="Arg81p"
/protein_id="EWG89221.1"
/translation="MGISSKNRPKKMGRAKTFTGCWTCRGRKVKCDLRHPHCQRCEKS
NLPCGGYDIKLRWSKPMQFDPYGVPIPQNSPATTTNLSGSVDEPQYQRRNIDFVRYDE
EYVYHEDMDDELTMLHTPPIEKISDNKTWIIKKFGVFKGTDKIDKQYAPRKKRNRKRV
AKSLESSASISLSSSPSSSTISFPIRHIEDKLRNKGHVKTGILSANDGVPPTPNLLDY
DWNNLNITGYEWISSELRDDALLSAVTLQGHHLGHTQPQEISLEENSNVVSGEEQVNA
KEHGCAVETDNQGSSTLPNKAASANDKLYQQNLKLLFQKNSSNSEEPDPQALIDDVFV
NIEPRSLPASDLNKITLAPPNEESRMPKSMLELTSYSSDLPPELVDIIPKTDLTVHGL
ARFLLNHYFNNVADKMTVVVLEKNPWKTLYFPRALMALGDLAGLGQSSNSRNALLNAL
LAVSCFHLQSKYPRNYKLQKYFLGLGIELRNQASNFLRLCLNTKSSIPEKYKDVLTAI
LSMNSIDVVWGTMADCQDHLALCEDFVESRMKLRPNISEKAKTLHRIFSFLKLIQDST
ALDKVRAKEIVILPSEEDDNYKLLDTSNATSSSSEPRVDVVQEGLFREALNENDGKIH
IEFVKEPITNVSADSTPSSTTPPIFTNIATESYYNKSDISNLVSKTDENIIGTDSLYG
LPNSLILLFSDCVRIVRHNEYYNLTYLPVPRKFNELSLNFEKRLLKWKSEWNFHQENS
EGKSFINSTAEALYHHTMSFYFSLIIYYFTMARSLNCQFLQNYVAKVLDHLNAMEELV
DQKKVKIVPLIWQGFMAGCACTDENRQQEFRRWAAKLAESGVGSYWGARQVMLEVWRR
RKEDEPGDNWYSIYKDWEMNLMLS"
gene 68485..68988
/gene="Taf13"
/locus_tag="P301_M10271"
mRNA 68485..68988
/gene="Taf13"
/locus_tag="P301_M10271"
/product="Taf13p"
CDS 68485..68988
/gene="Taf13"
/locus_tag="P301_M10271"
/note="Similar to YML098W TFIID subunit (19 kDa), involved
in RNA polymerase II transcription initiation, similar to
histone H4 with atypical histone fold motif of Spt3-like
transcription factors"
/codon_start=1
/product="Taf13p"
/protein_id="EWG89222.1"
/translation="MSRKLKKTNLFNKDVSSLLYAYGDVPQPLQATVQCLDELVSGYL
VDVCTNAFHTAQNSQRNKLRLEDFKFALRKDPIKLGRAEELIATNKLITEAKKQFNET
DNQNSLKRYREEDEEGDEMEEDEDEQQVTDDDEEGVGRNSAKQSTDSKATKIRKQAPK
NLKKTKK"
gene complement(69551..70906)
/gene="Vps9"
/locus_tag="P301_M10286"
mRNA complement(69551..70906)
/gene="Vps9"
/locus_tag="P301_M10286"
/product="Vps9p"
CDS complement(69551..70906)
/gene="Vps9"
/locus_tag="P301_M10286"
/note="Similar to YML097C 'A guanine nucleotide exchange
factor involved in vesicle-mediated vacuolar protein
transport; specifically stimulates the intrinsic guanine
nucleotide exchange activity of Vps21p/Rab5: similar to
mammalian ras inhibitors; binds ubiquitin'"
/codon_start=1
/product="Vps9p"
/protein_id="EWG89223.1"
/translation="MTDDEKREILKEFDPFSQLEQANGNPDKDVKFKKDDPNKAAAEE
TNRDISAQDKGDEEPFYDFQIFIKQLQTPGADPLVKYTKSFLRNFLAQRLLWTVSEEI
KLISDFKTFIYDKFILYEPFKSLDNSKMRNAKEGMEKLIMGKLYSRCFSPSLYEILQK
PLDDEHMKDLTNDDTLLEKIRHYRFISPIMLDIPDTMPNARLNKFVHLASKELGKINR
FKSPRDKMVCVLNASKVIFGLLKHTKLEQNGADSFIPVLIYCILKGQVRYLVSNVNYI
ERFRSPDFIRGEEEYYLSSLQAALNFIMSLTERSLTIEDHEDFEEAYQRNLKQLAEEK
EEEEKKKQLEVPDELQPNGTLLKPLDEVTNIVISKFNELFSPIGEPTQEEALKSEQSN
KEEDVSSLIKKIEENERKDTLNTLQNMFPDMDPSLIEDVCIAKKSRIGPCVDALLSLS
E"
gene 71126..72703
/locus_tag="P301_M10291"
mRNA 71126..72703
/locus_tag="P301_M10291"
/product="hypothetical protein"
CDS 71126..72703
/locus_tag="P301_M10291"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89224.1"
/translation="MCGILLHYCPNNNHLNDELIEFPEGTEFGDTTCTNESSIFNKII
PYIAARGPNYSSLRAVKAYRISWFSSVLSLRQPFTKQSINVDDRYFLQFNGELYNKEI
SQGDNDSLYIASMLQNLKEGMGVIDVIKSLEGEYAYTIYDVNSSKLYFGRDPIGRRSL
SYSVTPDNELYVASVTGSAGSFQDCIGGVIYEYDTRTKLLNSNQRSYLPYEVTSEIDL
NFTSLSEVSKNLYAVLRDSVKKRVESIHPRHIENSPIAVLFSGGIDCSVIVALICEVL
QENDYKCGKPVIELLNVSFENPRTGLFPSDTPDRKLSINSAKTLQNLYPNIDIKLVEV
DVPYDEYLKWKPFVINLMYPKQTEMDLSIAIAFFFASRGRGFLTSLNGERTPYQRHGI
VLFSGLGADELYGGYHKFANKPPHELVEELTRQINNIYDRNLNRDDKVIAHNGVEVRY
PFLDEYVIKLSTAEIPINFKVNKLILRKVASQYLKLDGISSEPKRAIQFGAKSAKMTK
DGNKHGTDLLKENRNCS"
assembly_gap 72708..72924
/estimated_length=217
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<72925..73331)
/gene="Rad10"
/locus_tag="P301_M10301"
mRNA complement(<72925..73331)
/gene="Rad10"
/locus_tag="P301_M10301"
/product="Rad10p"
CDS complement(<72925..73331)
/gene="Rad10"
/locus_tag="P301_M10301"
/note="Similar to YML095C 'Single-stranded DNA
endonuclease (with Rad1p), cleaves single-stranded DNA
during nucleotide excision repair and double-strand break
repair; subunit of Nucleotide Excision Repair Factor 1
(NEF1); homolog of human ERCC1 protein'"
/codon_start=1
/product="Rad10p"
/protein_id="EWG89578.1"
/translation="MNNTDPTSFESILAGVAKLRKEKSGADTTGSQSLEIDASKLQQQ
EPQTSRRINSNQVINAFNQQKPEEWTDSKATDDYNRKRPFRSTRPGKTVLVNTTQKEN
PLLNHLKSTNWRYVSSTGINMIYYDYLVRGRSVL"
gene complement(73437..73838)
/locus_tag="P301_M10306"
mRNA complement(73437..73838)
/locus_tag="P301_M10306"
/product="hypothetical protein"
CDS complement(73437..73838)
/locus_tag="P301_M10306"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89225.1"
/translation="MSTMNFLLSTILPGMYNDADAGTSSFCPSFPAWETVLISSIHSV
NLPLAMVNACKDCVKCCNSWSNCCFTAFSCSGFSLVKSTKFRTCSNLKVSKVSVRWQQ
RRSYSFTYFFERTWLDFYSALSCLGHSKIFI"
gene 73493..74067
/gene="Gim5"
/locus_tag="P301_M10311"
mRNA join(73493..73508,73592..74067)
/gene="Gim5"
/locus_tag="P301_M10311"
/product="Gim5p"
CDS join(73493..73508,73592..74067)
/gene="Gim5"
/locus_tag="P301_M10311"
/note="Similar to YML094W Subunit of the heterohexameric
cochaperone prefoldin complex which binds specifically to
cytosolic chaperonin and transfers target proteins to it"
/codon_start=1
/product="Gim5p"
/protein_id="EWG89226.1"
/translation="MSSQKIDLTKLNPEQLNAVKQQFDQELQHFTQSLQALTMAKGKF
TECIDDIKTVSQAGNEGQKLLVPASASLYIPGKIVDNKKFMVDIGTGYYVEKSAEAAI
AFYQKKVDKLNKESVQIQDIIKEKTQYSLSIEAQIRQAAIRQHEAMSKQQQQQQKKES
STA"
gene 74308..77006
/gene="Utp14"
/locus_tag="P301_M10316"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(77203..77787)
/gene="Pre8"
/locus_tag="P301_M10321"
mRNA complement(77203..77787)
/gene="Pre8"
/locus_tag="P301_M10321"
/product="Pre8p"
CDS complement(77203..77787)
/gene="Pre8"
/locus_tag="P301_M10321"
/note="Similar to YML092C Alpha 2 subunit of the 20S
proteasome"
/codon_start=1
/product="Pre8p"
/protein_id="EWG89227.1"
/translation="MSETLSKVSLLTPDIGAVYSGMGPDYRVLVDKSRKVAHTSYKRI
YGEYPPTKLLVSEVAKIMQEATQSGGVRPFGVSLLIAGHDEFNGFSLYQVDPSGSYFP
WKATAIGKGSVAAKTFLEKRWNDELELEDAIHIALLTLKESVEGEFNGDTIELAIIGD
ENPDLLGYTGIPTDKGPRFRKLTSQEINDRLEAL"
assembly_gap 77926..77961
/estimated_length=36
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(78285..81892)
/gene="Rpm2"
/locus_tag="P301_M10326"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 83396..85402
/gene="Ufo1"
/locus_tag="P301_M10346"
mRNA 83396..85402
/gene="Ufo1"
/locus_tag="P301_M10346"
/product="Ufo1p"
CDS 83396..85402
/gene="Ufo1"
/locus_tag="P301_M10346"
/note="Similar to YML088W 'F-box receptor protein, subunit
of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase
complex; binds to phosphorylated Ho endonuclease, allowing
its ubiquitylation by SCF and subsequent degradation'"
/codon_start=1
/product="Ufo1p"
/protein_id="EWG89228.1"
/translation="MERPGLVLQDLPPEILINIFSHLDEKDLFTLQELSTHFRNLIHD
EELWKNLFKSRVHTTHFPTFSQSSKFSVEYIERTRGLHHWQHNKAIRTKYTIIPTRNW
DQPSIERIVFDYPRVAAYNDGTITILQLQNHKRQKKFKKLIYIPCTTPQGCSTMDFNI
NAAVFGRFDGRVFGKLLSNKSYLTPVMEFTGRHSAGVTAICNSESWDTSREDWSVSGS
ENGEIIWWCENKLVKMWKVSNRVIWKLAFFKDWTLIMDDEKLYIIHQMQELHSIDIPK
DLDEQPMRVRFFKMDFGSMTLVLADLNNVYTISVNPNGNFGNLRKLEMPEQICAVEID
EKTSQREQNWQFAGDDGCYISLLTTQNTLYIINIRDLSSSGLKVQCKISFDEQVYVSQ
VTNLIVVVALPNVLQILNAMTGELIKTVLKTEKFPEFLKVSQDKIIMGSGNVLNYLKF
VSSDSKKHHHSTKGKNTVSNKWNETLNTELQYYDEDEDLRRKRQSEISRLIDAYGGDL
ELSGDTDEENDIQLRIALLESQEAQARNQAEAGEPVGDDEDEQLRRALEESQLIYETQ
TNSSANHGNSTNDEIDEDDEEFLRAIRQSRVEDERRRHLRNHTTGRRNGPLSDDNFAT
YGAAESSERTSTENTIGSSVGVDASNNVDEDLQLAIALSLSEIN"
gene complement(85590..86528)
/gene="Aim33"
/locus_tag="P301_M10351"
mRNA complement(85590..86528)
/gene="Aim33"
/locus_tag="P301_M10351"
/product="Aim33p"
CDS complement(85590..86528)
/gene="Aim33"
/locus_tag="P301_M10351"
/note="Similar to YML087C 'Putative protein of unknown
function, highly conserved across species and orthologous
to human CYB5R4; null mutant displays reduced frequency of
mitochondrial genome loss'"
/codon_start=1
/product="Aim33p"
/protein_id="EWG89229.1"
/translation="MSIVETCISFVSTNPFYPFCTGLLLNCVVTPLYFWKTQNGRIVV
VSLLQFVVLYATAFISIGTDKSLYRNKWVALPLSKKTRISRNTSLYCFKLKYPFERLH
IPMGYHLAVRVTINGERLVRYYTPVNVPNTEGHLELVVKTYKHGVVSKYFDKLKIGQY
VEFKGPLGELEYDQDTATELGIIAGGSGITPVLQVLQEIIPSPEDLTHISLIYANETE
DDILMKSQLDHMAKEYPHFKVHYVIHKPNGKWNGDVGYVTLEEMKRYLPKQAEDHRLL
ICGPPKMNEMVLNYAKELGWSNGFHKGNGTDKVFVF"
assembly_gap 86779..86950
/estimated_length=172
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(86952..88532)
/gene="Alo1"
/locus_tag="P301_M10356"
mRNA complement(86952..88532)
/gene="Alo1"
/locus_tag="P301_M10356"
/product="Alo1p"
CDS complement(86952..88532)
/gene="Alo1"
/locus_tag="P301_M10356"
/note="Similar to YML086C D-Arabinono-1,4-lactone oxidase,
catalyzes the final step in biosynthesis of
dehydro-D-arabinono-1,4-lactone, which is protective
against oxidative stress"
/codon_start=1
/product="Alo1p"
/protein_id="EWG89230.1"
/translation="MSTIPFRKNYVFKNWAGIYSAKPERYFQPSSIDEVVELVKSARL
AEKSLVTVGSGHSPSNMCVTDEWLVNLDRLDKVQKFVEYPELHYADVTVDAGMRLYQL
NEFLGAKGYSIQNLGSISEQSVAGIISTGSHGSSPYHGLISSQYVNLTIVNGKGELKF
LDAENDPEVFKAALLSVGKIGIIVSATIRVVPGFNIKSTQEVITFENLLKQWDTLWTS
SEFIRVWWYPYTRKCVLWRGNKTTDAQNGPAKSWWGTKLGRFFYETLLWISTKIYAPL
TPFVEKFVFNRQYGKLEKSSTGDVNVTDSISGFNMDCLFSQFVDEWGCPMDNGLEVLR
SLDHSIAQAAINKEFYVHVPMEVRCSNTTLPSEPLDTSKRTNTSPGPVYGNVCRPFLD
NTPSHCRFAPLENVTNSQLTLYINATIYRPFGCNTPIHKWFTLFENTMMVAGGKPHWA
KNFLGSTTLAAGPVKKDTDYDDFEMRGMALKVEEWYGEDLKKFRKIRKEQDPDNVFLA
NKQWAIINGIIDPSELSD"
assembly_gap 88827..88853
/estimated_length=27
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(89101..90557)
/gene="Tub1"
/locus_tag="P301_M10366"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(90945..92201)
/locus_tag="P301_M10376"
mRNA complement(90945..92201)
/locus_tag="P301_M10376"
/product="hypothetical protein"
CDS complement(90945..92201)
/locus_tag="P301_M10376"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89231.1"
/translation="MDYLHVYNNNQAHVPRTPPGRQCLSPVLPPIILALDQQHSLTFG
YSSPRCLPTPILPSMSTNTPVHWSQQVVTIPVAVRDHAGMMPIITPMTSPRPQFEATA
PSPPLAPVAIKLPDLKLPPSPVPSTVEDRVSHPILPKIVIGNGGGRDVERDSTEELIR
KIPNYIDCAKTKAQLSKVRSGKQLIACAQEYHHPVNKDEIENINNILNFRDFIFKHPK
SSFEFLCTLSFEQFVRVYSFISFIYRTKKINKNKYELVCEMNVHEQLSNKRIQRTRTP
EKYKIHLICESKLILTFNHCTKTVKFESINGGHCHPISANHIIKPSLFLTHCINKCHQ
TVSDPTDLKLALRDALEALDHERIGLSFLKRRHFKCSHQASSLSNASATLKKQEDHNT
FGVHTGIIHTANFFMFNASSDIFQRN"
gene 93013..94962
/locus_tag="P301_M10391"
mRNA 93013..94962
/locus_tag="P301_M10391"
/product="hypothetical protein"
CDS 93013..94962
/locus_tag="P301_M10391"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89232.1"
/translation="MVSAQVATELGQPIPLDTQHAVSVCFPTWKSVISYVEKDPKVLG
CLKSGYPRFWIHPSIQKLRDILIEKYAKENETCFCFPSYRVAKRCREYVRRKCAHRNG
KVRILQLATAKPINEEQKTWKRECKIAVVFVDGAYENILKQYWQYTGEIISSRLAEYV
LHELFMVEKKSSPAEEKEYIEMRYGRNLNFAFADRAKELIKKRIATKVIDKDEHDEEE
NYHFLAGNQDEQDFQDTFLDSSLNEANHGEDHDGGISGEVDSQEEPHNGLVSTIPPEP
IEMSTIEEEQSVEEDAGRCALRVCPERDVFLFPSGMASIFTAHRLLLQWDSLRLNRSR
NGSDVTSSPPNKKTVIFGFPYADTLHVLQEFNETYFLGEGDESSMKELTKILHSGEQI
LAVFIETPSNPLLKMGNLLELKRLSELFGFFIIIDETVGGIVNIDGLPFADIVCSSLT
KTFSGDSNVIGGSMVLNPQSRVYEFASRFMQLEDEYEDLLWCEDAIYLERNSRDFIAR
TIRINYSTEYLLDKILKPHVGENKLFKKIYYPNLTSKETLTNYDMVRCKKEGGYGGLF
SLTFHDEDHAGAFYDNLKLNKGPSLGTNFTLAFPYTLMTYYHELDMAEKFGVERNLLR
ISVGLESQSILGKIFQEAIDKTVEI"
gene complement(95133..95312)
/gene="Atp18"
/locus_tag="P301_M10396"
mRNA complement(95133..95312)
/gene="Atp18"
/locus_tag="P301_M10396"
/product="Atp18p"
CDS complement(95133..95312)
/gene="Atp18"
/locus_tag="P301_M10396"
/note="Similar to YML081C-A no_gene_description"
/codon_start=1
/product="Atp18p"
/protein_id="EWG89233.1"
/translation="MLKRFPTPILKVYWPFFVAGAAVYYGMSKAADLSSNTKEFINDP
RNPRFAKGGKFVEVD"
gene 95927..99682
/locus_tag="P301_M10401"
mRNA 95927..99682
/locus_tag="P301_M10401"
/product="hypothetical protein"
CDS 95927..99682
/locus_tag="P301_M10401"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89234.1"
/translation="MSSEEFKGLPIKRDISSTIYADRPPALSAPPCVGATGNDKIQVL
PIPKKSRTIKTDKPRPFLCHICTRGFVRQEHLKRHQRAHTNEKPFLCVFCGRCFARRD
LVLRHQHKLHSALVSKESINSKDKTEIDAINDKNIIQIQGNKQTILPTPSNPLAKTAA
QLKKAAKEKKNGKQGKLDLSPSYRANNHSTDVSPSVGNSSTPAVIEETDSSSHFPLPD
TNIPTKSKRHASFSASSAFTYSSDNFQKLHQQAKSDFDELQESIPHQVGFSTPQLTAQ
QLIENAIESGVVDLETLDLPPFLSLDGLPPASSSTAVAASEQIDICPSSATDTISGAN
STPNQAATAPPFQLPIARESSSLFLANTPYLSDFLTMGSSYGGSGGFAKSITADPSLD
YFNYKNHSHPDSRHNNSSSGINYSNNKNNNESIEKSQNNSNVINETIDHTDIHAHHAD
AHDDSFIESEEWLSKFIMDSQIDNDLKLNINHFNDIGFNNLHPQNPTTHSEPRNMHNE
NRDMHRSASKFQSVSENISPREQMSLFKTKQNKAISKFLSDEKIPSTASPSSSASPVQ
FDKKNVDINEFLLDESVSNLFTTRQIDLFKKNVNLYSPLFQNQKDAVSSTSLTPSLTT
QTATTQSGPGWTDSSQKLTFFTEQLRNLIIKENNLKSNLFPTVDELNHYVDLYQVEFH
KYFPFIHLYSIIPSSENYPLVISISMIGALYGFHSTHALLLSKIARTRVRMFLENTRS
NHDKTPIWLMQSLVLLTFTSIFSNDMNAFRTVNTQIMILVQLIKISKLNYPLENFIKP
PIESDHVLEYQDNPAVLNQFKAQYNTREQINRNFKYFILAQSRIRICHIVLLISNLFK
SLVDFDCCFHSIDLKCGVPCYNEVLFFCENSRTWNENLTRFNIVLDSKFSLIEVSNGE
SNYEKCLMYLSNGNPYLYKNAKISFKTLLSLLISIHEKINIERDALKDSDESDFHAKN
VQWRMHSRPLVATMLKHWELLYIKNGGILALSDENLPIINTNPSFRLIIPLYFFAKLR
KCLDIAPTLRCIWNQDWNSMNSSLEKVCYEWESLREATEYAVSVITFWIDTVSVMKGK
STQTPIFTITCIFVSILVIAGYMRRLEDFAQNKNSDCMIGSLKSTDRILWLKAFKTLK
RIESHLSEREYKLQTFAEFLRVPDNGSLDIESLDSSLIENTLNSHDVTNQALDIITRT
RLSSRTLYCGARILGDTPVWPVSLLFAHALQSRAIYNINHRKSVNSV"
gene 99953..101224
/gene="Dus1"
/locus_tag="P301_M10406"
mRNA 99953..101224
/gene="Dus1"
/locus_tag="P301_M10406"
/product="Dus1p"
CDS 99953..101224
/gene="Dus1"
/locus_tag="P301_M10406"
/note="Similar to YML080W 'Dihydrouridine synthase, member
of a widespread family of conserved proteins including
Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17'"
/codon_start=1
/product="Dus1p"
/protein_id="EWG89235.1"
/translation="MTEPALSSANNALMQKLTGRQLFDKIGRPTRIVAPMVDQSELAW
RILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDGSSVDRPLVVQFCANDPEYLL
AAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLIHNLINTLHKNLKVPVTAK
IRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLANWETIKYLRDNLPKET
VFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVDQTKNKEKIFPRVDKII
REYFRIVKECQESKASKTAMKSHFFKILRPFLPHHTDIRSTLATMNAKATWEEWEEQV
VKPVEKVVQEIFEQPDIDIKDEITIGEKQSWGGSYRTVPYWRCQPYFRPVNGITGDKR
VMQGLIDESVNKKRKADVPLESADKKKDVKA"
gene 101394..101999
/locus_tag="P301_M10411"
mRNA 101394..101999
/locus_tag="P301_M10411"
/product="hypothetical protein"
CDS 101394..101999
/locus_tag="P301_M10411"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89236.1"
/translation="MSANVQEAANAAIEPASFVKVPMPEPPSSLQQLINDWQLIKHRE
GGYFKETDRSPYTMEVEKPVNGGSGNTEMVTRNQSTLIYYLLTPDSPIGKFHKNINRI
IHILQRGKGQYVLVYPDGQVKSFKVGFDYKNGEVSQWVVPGGVFKASFLLPNEEFDNG
FLISEVVVPGFDFEDHTFLKGEDELKHLVGPEKAAELAFLA"
gene 102161..102709
/gene="Cpr3"
/locus_tag="P301_M10421"
mRNA 102161..102709
/gene="Cpr3"
/locus_tag="P301_M10421"
/product="Cpr3p"
CDS 102161..102709
/gene="Cpr3"
/locus_tag="P301_M10421"
/note="Similar to YML078W 'Mitochondrial peptidyl-prolyl
cis-trans isomerase (cyclophilin), catalyzes the cis-trans
isomerization of peptide bonds N-terminal to proline
residues; involved in protein refolding after import into
mitochondria'"
/codon_start=1
/product="Cpr3p"
/protein_id="EWG89237.1"
/translation="MFKRSIIQQSRLFSNSASRLGKKVFFDPAVNGTKIGRIEFELYD
NVVPKTAENFRALCTGEKGWGYKGVPFHRIIPDFMIQGGDTDLTNGFGGKSIYGSKFA
DENFVKKHDKAGLLSMANAGPNTNGSQFFITTVPCPWLDGKHVVFGEVTKGMDIVKAI
ESYGTASGKPRAEIVIEEAGEL"
gene 103022..103501
/gene="Bet5"
/locus_tag="P301_M10426"
mRNA 103022..103501
/gene="Bet5"
/locus_tag="P301_M10426"
/product="Bet5p"
CDS 103022..103501
/gene="Bet5"
/locus_tag="P301_M10426"
/note="Similar to YML077W Component of the TRAPP
(transport protein particle) complex, which plays an
essential role in the vesicular transport from endoplasmic
reticulum to Golgi"
/codon_start=1
/product="Bet5p"
/protein_id="EWG89238.1"
/translation="MGIYSFWIFDRHCNCIFDREWTLASNSASGTINSKQNEEDAKLL
YGMIFSLRSITQKLSKGSVKNDIRSISTGKYRVHTYCTASGLWFVLLSDFKQQSYTQV
LQYIYSHIYVKYVSNNLLSPYDFAENENEMRGQGTRKITNRNFISVLESFLAPMVNQ"
gene complement(103670..106504)
/gene="War1"
/locus_tag="P301_M10431"
mRNA complement(103670..106504)
/gene="War1"
/locus_tag="P301_M10431"
/product="War1p"
CDS complement(103670..106504)
/gene="War1"
/locus_tag="P301_M10431"
/note="Similar to YML076C 'Homodimeric Zn2Cys6 zinc finger
transcription factor; binds to a weak acid response
element to induce transcription of PDR12 and FUN34,
encoding an acid transporter and a putative ammonia
transporter, respectively'"
/codon_start=1
/product="War1p"
/protein_id="EWG89239.1"
/translation="MDTQIAITGVAVGKEINNNNSKTDQKVSLPKADVPCIDKATQTI
IEGCSKDDPRLSYPTKLETTEKGKTKRNSFACVCCHSLKQKCEPSDVNDIYRKPCRRC
LKHKKLCKFDLSKRTRKRKPRSRSPTSFESPMVNVSTKSKGPTDSEESSLKDGTSYLA
SFPSDPNAKQFPNSRTVLPGLQQSLSDLWSTLSQPPSYGAREAETTSTGEITTNNHTK
SNGSVPTNPAVLASNDEHTNISDAPVIYSTYNSPVPISSAPTSINSEALFKHRPKIVG
DEETQNVKVKRQKKSYSRHMTRSFRKQLQSLIISQKGKIRDISMKLDTWSKQWNDLVE
KSMFLPTIADPVSVGIISHEEATLRLHLYKTEISYLSKLPFIKVEENVSVDELRKKKP
ILFSVIMSCVSIVLTPKQTTRGTIMKLDSFVLNLITNQIFKANNKSIEIIESLSTLCL
WYNFFEWSSKTRYHIFNYICCCLTRDLGPTYVNRSFGMFSDEDPKRFKSPLELYSNGA
SLTLLVYISALNISIFLRQSIQARWSHVTEKACEDLVKETKKSRHYDNDKLLLDSADD
PILVQFAKMNHVLENIHTHLHERDLNDDEFDDPIFTKKYLNKLMEKYHKQLQEIFTKL
DRNRPRVIAFYYSVEAYLYQYKLAVFIGEMSHTINEKVELPREIMDDFVKCYHCCKSA
LEEFSKLEPILITSLPLFHTSRIIYTVGMLLLKLRYSVVAIPSFHDLMPLTDDAIALV
IGVNNLLEKTSELYPFNNSLYKFRYVIALFCQTYANKVIDVADRYNAEREKLKEKQVI
DEVSNGHDGTKPINAYVTESQKMPTEEDPIIDNNTNQNITAVPDEMLPVYSRVRDDTA
AMNLNINSTSYMNESPHEHRESMTGTTLLPPPFISNGVTNSADSTNIKPSPSSSVDNL
NDYLTDINSLAWGVNSLNDEFWTDLFMNDI"
gene complement(106892..110056)
/gene="Hmg1"
/locus_tag="P301_M10441"
mRNA complement(106892..110056)
/gene="Hmg1"
/locus_tag="P301_M10441"
/product="Hmg1p"
CDS complement(106892..110056)
/gene="Hmg1"
/locus_tag="P301_M10441"
/note="Similar to YML075C 'One of two isozymes of HMG-CoA
reductase that catalyzes the conversion of HMG-CoA to
mevalonate, which is a rate-limiting step in sterol
biosynthesis; localizes to the nuclear envelope;
overproduction induces the formation of karmellae'"
/codon_start=1
/product="Hmg1p"
/protein_id="EWG89240.1"
/translation="MPLLFKGLKQMAKPIAYVSRFSAKRPIHIILFSLIISAFAYLSV
IQYYFNGWQLDSNSVFETAPNKDSNTLFQECSHYYRDSSLDGWVSITAHEASELPAPH
HYYLLNLNFNSPNETDSIPELANTVFEKDNTKYILQEDLSVSKEISSTDGTKWRLRSD
RKSLFDVKTLAYSLYDVFSENVTQADPFDVLIMVTAYLMMFYTIFGLFNDMRKTGSNF
WLSASTVVNSASSLFLALYVTQCILGKEVSALTLFEGLPFIVVVVGFKHKIKIAQYAL
EKFERVGLSKRITTDEIVFESVSEEGGRLIQDHLLCIFAFIGCSMYAHQLKTLTNFCI
LSAFILIFELILTPTFYSAILALRLEMNVIHRSTIIKQTLEEDGVVPSTARIISKAEK
KSVSSFLNLSVVVIIMKLSVILLFVFINFYNFGANWVNDAFNSLYFDKERVSLPDFIT
SNASENFKEQAIVSVTPLLYYKPIKSYQRIEDMVLLLLRNVSVAIRDRFVSKLVLSAL
VCSAVINVYLLNAARIHTSYTADQLVKTEVTKKSFTAPVQKASTPVLTNKTVISGSKV
KSLSSAQSSSSGPSSSSEEDDSRDIESLDKKIRPLEELEALLSSGNTKQLKNKEVAAL
VIHGKLPLYALEKKLGDTTRAVAVRRKALSILAEAPVLASDRLPYKNYDYDRVFGACC
ENVIGYMPLPVGVIGPLVIDGTSYHIPMATTEGCLVASAMRGCKAINAGGGATTVLTK
DGMTRGPVVRFPTLKRSGACKIWLDSEEGQNAIKKAFNSTSRFARLQHIQTCLAGDLL
FMRFRTTTGDAMGMNMISKGVEYSLKQMVEEYGWEDMEVVSVSGNYCTDKKPAAINWI
EGRGKSVVAEATIPGDVVRKVLKSDVSALVELNIAKNLVGSAMAGSVGGFNAHAANLV
TAVFLALGQDPAQNVESSNCITLMKEVDGDLRISVSMPSIEVGTIGGGTVLEPQGAML
DLLGVRGPHATAPGTNARQLARIVACAVLAGELSLCAALAAGHLVQSHMTHNRKPAEP
TKPNNLDATDINRLKDGSVTCIKS"
gene complement(111247..112473)
/gene="Fpr3"
/locus_tag="P301_M10451"
mRNA complement(111247..112473)
/gene="Fpr3"
/locus_tag="P301_M10451"
/product="Fpr3p"
CDS complement(111247..112473)
/gene="Fpr3"
/locus_tag="P301_M10451"
/note="Similar to YML074C 'Nucleolar peptidyl-prolyl
cis-trans isomerase (PPIase); FK506 binding protein;
phosphorylated by casein kinase II
(Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p'"
/codon_start=1
/product="Fpr3p"
/protein_id="EWG89241.1"
/translation="MSDLLPLATYSLNVEPYTPVPAIDVTMPITVRITMAALNPEAID
EENKPSTLRIIKRNPDFEDDDFLGGDFDEDEIDEESSEEEEEEKTQKKKKSKGKKAES
ESEDDEEDDDEDDEFQESVLLTLSPEAQYQQSLDLTITPEEEVQFIVTGSYAISLSGN
YVKHPFDTPMGVEGEDEDEDADIYDSEDYDLTPDEDEIIGDDMDDLDDEEEEEVRIEE
VQEEDEEDNDGEEEQEEEEQKEEVKPEPKKSKKEKKRKHEEKEEEKKAKKVKKVEFKK
DLEEGPTKPKSKKEQDKHKPKSKVLEGGIVIEDRTIGNGPQAKRGARVGMRYIGKLKN
GKVFDKNTSGKPFAFKLGRGEVIKGWDIGVAGMSVGGERRIIIPAPYAYGKQALPGIP
ANSELTFDVKLVSMKN"
gene complement(114375..115321)
/gene="Rpl6a"
/locus_tag="P301_M10466"
mRNA complement(join(114375..114890,115307..115321))
/gene="Rpl6a"
/locus_tag="P301_M10466"
/product="Rpl6ap"
CDS complement(join(114375..114890,115307..115321))
/gene="Rpl6a"
/locus_tag="P301_M10466"
/note="Similar to YML073C 'N-terminally acetylated protein
component of the large (60S) ribosomal subunit, has
similarity to Rpl6Bp and to rat L6 ribosomal protein;
binds to 5.8S rRNA'"
/codon_start=1
/product="Rpl6ap"
/protein_id="EWG89242.1"
/translation="MSAQKAPKWYPSEDVAALKKTRKAARPQKLRASLVPGTVLILLA
GRFRGKRVVYLKHLEDNTLLISGPFKVNGVPLRRVNARYVIATSTKVSVEGVNVEKFN
VEYFAKEKLTKKEKKEANLFPEQQNKEIKAERVEDQKVVDKALIAEIKKTPLLKQYLS
ASFSLKNGDKPHMLKF"
gene complement(115880..120517)
/gene="Tcb3"
/locus_tag="P301_M10471"
mRNA complement(115880..120517)
/gene="Tcb3"
/locus_tag="P301_M10471"
/product="Tcb3p"
CDS complement(115880..120517)
/gene="Tcb3"
/locus_tag="P301_M10471"
/note="Similar to YML072C 'Lipid-binding protein,
localized to the bud via specific mRNA transport;
non-tagged protein detected in a phosphorylated state in
mitochondria; GFP-fusion protein localizes to the cell
periphery; C-termini of Tcb1p, Tcb2p and Tcb3p interact'"
/codon_start=1
/product="Tcb3p"
/protein_id="EWG89243.1"
/translation="MTGIKAQVHPPPDSTLFHEEEKKKVGGNLPQKVINQQERGSDHA
PSGHHQYHQLINHDANDTKTSNSVSDVSKGQKTADSNPEGKKQSSKDIFVASSAQKTN
QLPGPNPQGSIGAVPLEDLRPKEFRSAPSRKPNKFDTSITKPGVLDDLGKLDEKDIKE
KFHPDSDDKLFPWQNVGEFHASGKGSPNTKMSRVIKAYILENFYNDWYCNIATVLGTC
FFSWLFAYIGFSWWSMIFIFLGTATVYNAEYTRFNRNIRDDLKRVTVEETLSDRVEST
TWLNSFLSKFWVIYMPVLSQQVKDNVNPQLAGVAPGYGIDALAIDEFTLGSKAPTIKG
IKSYTKTGKNTVEMDWSFAFTPSDVSDMTATEAREKINPKISLGVTLGKSFVSKTMPI
LVEDINVAGKMRIKVEFGKAFPNIKIVSLQLLEPPLIDFALKPIGGDTLGLDVMSFLP
GLKSFVKNIINSNIGPMLFPPNHLDINVEDIMAAQSKEAIGVLAVTIASADSLKGSDF
ITNTVDPYIVMTTEDAVPGTDEEVRTSIKSNVKNPRWNETKYLLLNTLEQKLNLKCFD
FNDVRKDTVIGDLQLDLADLLQNPVLENQTAELRSGTKSKGILHYSLHWFPVKEDKSE
EKAVERAEAKAKGKKEDENEDTAEKEEDENEESSQTDVGIAKITLQKVKYLDTTSSMT
GSLSPCAELFIDGQKVKSYRTLRRINEPSWNETIEVLVPSKSNSKFVLKIFDDRMNGK
ALICEYSSSLDDIMSTLDAAQEFVKGSPQGDIYLDVSWKSIEMTGAFAAANSVSEPIG
CIKLDVKDAIIKGDLSGVGDVDPYYTVSLNRRVLYKSIYHSDTDHPIFDNSTYVPIFS
PNQILTLEFHDYQKIGKDRFIGSVQIPTSNVFKKDPKSGKYVGNNDKEEISKLKLKDH
KHKVTESIVNVSTTFIPINLVYSPEELVNVEKLEKELKEKKKKFEATQEENEQEMEKN
PKEWEVAEIEDPFDSDEKKINRKAKLSLNELIKQKSGILSMQILEGTLSPSSAYLEIL
ADDISYPVFICMKPSQGKLNSEMANIFIRDLNYSKLHFRVSKKHIAKDSDDVISETSY
STLKLLKQAYEEPMWLNFNGSKMKVRFLYTPTSVKLPSSESVEDTGYLNIKLISGHGL
KSADRNGYSDPFVHIYVNGKKVFKSNIKKKTLDPVWNEDAKIPILSRSKNQVIFNVLD
WDRAGDNDDLGQASLDVSSLEVGKTYHWNLNLNTQGSIRLQGSFNPEYIKPSFDIVKG
GITDKPMKIASGAAHATVGIAGTGIGAATGVATGGLKKGGHLLKSLGGNPMKRSKSSN
GNESNGAKKSSEKKSFDRRSPSNLNSTSVTPRASLDYDPSVPNTSYAPVQSASPAVKP
TDNTSSSSNKKDTPSSNSRGHSRASSFARTLAPHGTYNGFITVVAAENVAKHVQIKIS
LTQGGRLKHIYKTKSQKANNDGVAVFDEECSFKASPEANLVLGAISHQRLSRDKDLGI
AQINLGDPQIQQDGQISVKLGDGHLIVKINYGKDKNSQVPPVPEVPQEYTQ"
gene complement(120864..122721)
/gene="Cog8"
/locus_tag="P301_M10486"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 121536..121570
/estimated_length=35
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(122974..123045)
/locus_tag="P301_M10496"
tRNA complement(122974..123045)
/locus_tag="P301_M10496"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg3);
TR(UCU)M2"
gene 124604..126358
/gene="Dak1"
/locus_tag="P301_M10511"
mRNA 124604..126358
/gene="Dak1"
/locus_tag="P301_M10511"
/product="Dak1p"
CDS 124604..126358
/gene="Dak1"
/locus_tag="P301_M10511"
/note="Similar to YML070W 'Dihydroxyacetone kinase,
required for detoxification of dihydroxyacetone (DHA);
involved in stress adaptation'"
/codon_start=1
/product="Dak1p"
/protein_id="EWG89244.1"
/translation="MSAKSFEVTDPVNSSLKGFALANPSITLVPEEKILFRKTDSDKI
ALISGGGSGHEPTHAGFIGKGMLSGAVVGEIFASPSTKQILNAIRLVNENASGVLLIV
KNYTGDVLHFGLSAERARALGINCRVAVIGDDVAVGREKGGMVGRRALAGTVLVHKIV
GAFAEEYSSKYGLDGTAKVAKIINDNLVTIGSSLDHCKVPGRKFESELNEKQMELGMG
IHNEPGVKVLDPIPSTEDLISKYMLPKLLDPNDKDRAFVKFDEDDEVVLLVNNLGGVS
NFVISSITSKTTDFLKENYNITPVQTIAGTLMTSFNGNGFSITLLNATKATKALQSDF
EEIKSVLDLLNAFTNAPGWPIADFEKTSAPSVNDDLLHNEVTAKAVGTYDFDKFAEWM
KSGAEQVIKSEPHITELDNQVGDGDCGYTLVAGVKGITENLDKLSKDSLSQAVAQISD
FIEGSMGGTSGGLYSILLSGFSHGLIQVCKSKDEPVTKEIVAKSLGIALDTLYKYTKA
RKGSSTMIDALEPFVKEFTASKDFNKAVKAAEEGAKSTATFEAKFGRASYVGDSSQVE
DPGAVGLCEFLKGVQSAL"
gene 126631..128289
/gene="Pob3"
/locus_tag="P301_M10516"
mRNA 126631..128289
/gene="Pob3"
/locus_tag="P301_M10516"
/product="Pob3p"
CDS 126631..128289
/gene="Pob3"
/locus_tag="P301_M10516"
/note="Similar to YML069W Subunit of the heterodimeric
FACT complex (Spt16p-Pob3p), which associates with
chromatin via interaction with Nhp6Ap and Nhp6Bp, and
reorganizes nucleosomes to facilitate access to DNA by RNA
and DNA polymerases"
/codon_start=1
/product="Pob3p"
/protein_id="EWG89245.1"
/translation="MSTDFDRIYLNQSKFSGRFRIADSGLGWKISTSGGSAANQARKP
FLLPATELSTVQWSRGCRGYDLKINTKNQGVIQLDGFSQDDYNLIKNDFHRRFNIQVE
QREHSLRGWNWGKTDLARNEMVFALNGKPTFEIPYARINNTNLTSKNEVGIEFNIQDE
EYQPAGDELVEMRFYIPGVIQTNVDENMTKKEESSNEVVPKKEDGAEGEDVQMAVEEK
SMAEAFYEELKEKADIGEVAGDAIVSFQDVFFTTPRGRYDIDIYKNSIRLRGKTYEYK
LQHRQIQRIVSLPKADDIHHLLVLAIEPPLRQGQTTYPFLVLQFQKDEETEVQLNLED
EDYEENYKDKLKKQYDAKTHIVLSHVLKGLTDRRVIVPGEYKSKYDQCAVSCSFKANE
GYLYPLDNAFFFLTKPTLYIPFSDVSMVNISRAGQTSTSSRTFDLEVVLRSNRGSTTF
ANISKEEQQLLEQFLKSKNLRVKNEDREVQERLQTALGSDSDEEDINMGSAGEDDESV
DEDFQVSSDNDADEVAEEFDSDAALSDAEGGSDEERPSKKPKVE"
gene 128681..130075
/gene="Itt1"
/locus_tag="P301_M10521"
mRNA 128681..130075
/gene="Itt1"
/locus_tag="P301_M10521"
/product="Itt1p"
CDS 128681..130075
/gene="Itt1"
/locus_tag="P301_M10521"
/note="Similar to YML068W Protein that modulates the
efficiency of translation termination, interacts with
translation release factors eRF1 (Sup45p) and eRF3
(Sup35p) in vitro, contains a zinc finger domain
characteristic of the TRIAD class of proteins"
/codon_start=1
/product="Itt1p"
/protein_id="EWG89246.1"
/translation="MALTQFENDLEILRDMYPELEMKSVKVEEEGEFPQRINGKLLFK
ISLLADVNIEFDEQHMLLSNLSNECVEFTIYSCHYPDIRRCVVMDIKSLWISTDEKKM
LIDKALRLVEETVDMSIEFADSFTSILILIFGFLIDDTAILLFPNGIRKCLTQDQYDL
FKQIGEEATLQKMSRSNYHCCICMEMEKGVRMIKLPCENANVEHYLCRGCAKSYFTAM
IQENRISSVRCPQCEYKELKLEDFKSYKKMLKALFTPLIPVSFLKEVIDTELCERYEK
MFYNQAATRLSKYCPYACVTCRRCDSWCTKEDLDDAMIQCQKCHFVFCFDCLHAWHGY
NNKCGKKFSLSTDIIEEYLDDTVTSYERKRKLEAKYGRRVLELEVNDYLAEKMLDLAI
KKEGSNLQRCPKCKVVVERSEGCNKMKCEVCGMLFCFICGVLLYPEDPYEHFREAYSG
CYGRLFEGMPGTET"
gene complement(130194..131347)
/gene="Erv41"
/locus_tag="P301_M10526"
mRNA complement(join(130194..131221,131317..131347))
/gene="Erv41"
/locus_tag="P301_M10526"
/product="Erv41p"
CDS complement(join(130194..131221,131317..131347))
/gene="Erv41"
/locus_tag="P301_M10526"
/note="Similar to YML067C 'Protein localized to
COPII-coated vesicles, forms a complex with Erv46p;
involved in the membrane fusion stage of transport; has
homology to human ERGIC2 (PTX1) protein'"
/codon_start=1
/product="Erv41p"
/protein_id="EWG89247.1"
/translation="MAGLKTFDAFPKTEEQYKKKSTKGGLTSLLTYLFLLFIAWTEFG
EYFGGYIDQQYVVDSQVRDTVQINMDIYVNTKCDWLQINVRDQTMDRKLVLEELQLEE
MPFFIPYDTKVNDINEIITPELDEILGEAIPAEFREKLDTRSFFDESDPNRAHLPEFN
GCHIFGSIPVNRVSGELQITAKSLGYVASRKAPLEELKFNHVINEFSFGDFYPYIDNP
LDNTAQFNQDEPLTTYVYYTSVVPTLFKKLGAEVDTNQYSVNDYRYLYKDVAAKGDKM
PGIFFKYNFEPLSIVVSDVRLSFIQFLVRLVAICSFLVYCASWIFTLLDMALITIMGP
KWSLRYQPDDKTKGILDR"
gene complement(131557..132666)
/gene="Sma2"
/locus_tag="P301_M10536"
mRNA complement(131557..132666)
/gene="Sma2"
/locus_tag="P301_M10536"
/product="Sma2p"
CDS complement(131557..132666)
/gene="Sma2"
/locus_tag="P301_M10536"
/note="Similar to YML066C 'Meiosis-specific prospore
membrane protein; required to produce bending force
necessary for proper assembly of the prospore membrane
during sporulation'"
/codon_start=1
/product="Sma2p"
/protein_id="EWG89248.1"
/translation="MLFPKRLIVWGVLLILSLSQFVLYLPATTCTNSKGLRLCAPQFT
ITVIGGSSTANEFIASVREFLRLISYLTIDMGWSNEFTDPSVYEDENLVDTFQPDKVF
ELNYFGFCKRSNKSKVYCTSNENYGMDVLEVLVRDVGIQLGNISTTRSNETKKFGDSL
VLTYRLALTSIRDFLKHDKHTGNALSKALIGSPDPNVKGASPTKNYLKGVNLAFILMM
FNGMVFYFAVLEIIVGFLSICVVSAFGGALSVGKRHRLFPILLKSSSSILVVIATLTI
LCNIVYLIALKTLEPEEVTDVGSDNAAVHTTGWELLKVNVGSGFIMGLARYAIQWVLL
VLAFLAANHYKAKPKKSDKYTEDTSNSPSPDLMEK"
gene 133341..136086
/gene="Orc1"
/locus_tag="P301_M10541"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(136271..137008)
/gene="Tem1"
/locus_tag="P301_M10546"
mRNA complement(136271..137008)
/gene="Tem1"
/locus_tag="P301_M10546"
/product="Tem1p"
CDS complement(136271..137008)
/gene="Tem1"
/locus_tag="P301_M10546"
/note="Similar to YML064C 'GTP-binding protein of the ras
superfamily involved in termination of M-phase; controls
actomyosin and septin dynamics during cytokinesis'"
/codon_start=1
/product="Tem1p"
/protein_id="EWG89249.1"
/translation="MATPSTGANNSIPAVRNQVEVQVGLVGDAQVGKTSLMVKYVQNI
YDKEYTQTLGVNFLKRKVSIRSTDIIFSIMDLGGQREFINMLPIATVGSSVIIFLFDL
TRPETLGSIKEWYRQAYGLNDSAIPILVGTKYDLLIDLDPEYQEQISRTSMKYAQVMN
APLIFCSTAKSINIQKIFKIALAKIFNLTLTIPEINEIGDPLLIYKHLGGQQHRHHNK
SQDRKSHNIRKPSSSPSSKAPSPGVNT"
gene 137607..138374
/gene="Rps1b"
/locus_tag="P301_M10551"
mRNA 137607..138374
/gene="Rps1b"
/locus_tag="P301_M10551"
/product="Rps1bp"
CDS 137607..138374
/gene="Rps1b"
/locus_tag="P301_M10551"
/note="Similar to YML063W 'Ribosomal protein 10 (rp10) of
the small (40S) subunit; nearly identical to Rps1Ap and
has similarity to rat S3a ribosomal protein'"
/codon_start=1
/product="Rps1bp"
/protein_id="EWG89250.1"
/translation="MAVGKNKRLSRGKKGLKKKVVDPFTRKEWFDIKAPSTFENRNVG
KTLVNKSTGLKNASDALKGRVVEVCLADLQGSEDHSFRKVKLRVDEVQGKNLLTNFHG
MDFTTDKLRSMVRKWQTLIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSH
IRAIRKVVSEILTREVQNSTLAQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLL
KQPKFDVGALMALHGEGSGEEKGKKVSGFKDEVLETV"
gene complement(138630..139808)
/gene="Mft1"
/locus_tag="P301_M10556"
mRNA complement(138630..139808)
/gene="Mft1"
/locus_tag="P301_M10556"
/product="Mft1p"
CDS complement(138630..139808)
/gene="Mft1"
/locus_tag="P301_M10556"
/note="Similar to YML062C 'Subunit of the THO complex,
which is a nuclear complex comprised of Hpr1p, Mft1p,
Rlr1p, and Thp2p, that is involved in transcription
elongation and mitotic recombination; involved in telomere
maintenance'"
/codon_start=1
/product="Mft1p"
/protein_id="EWG89251.1"
/translation="MPLSQKQIDQVRTKVHYSEVDTPFNKYLDILGKVTKLTGSIING
TLSNDDSKIEKLTEQNISQLKESAHLRFLDLQSSIDTKKVADENWETCQQETLAKLEN
LKDKLPDIKSIHSKLLLRIGKLQGLYDSVQVINREVEGLSEGRTSLVVTRAEWEKELG
TDLVKFLIEKNYLKLVDPGLKKDSSEERYRIYDDFSKGPKELESINASMKSDIENVRQ
EVSSYKEKWLRDAEIFGKITSIFKEELLKRDGLLNEAEGDNIDEDYESDEDEERKERF
KRQRSMVEVNTIENVDEKEESDHEYDDQEDEENEEEDDMEVDVEDIKEDNEVDGESSQ
QEDNSRQGNNEETDKETGVIEEPDAVNDAEEADSDHSSRKLGGTTSDFSASSSVEEVK
"
gene complement(140078..142657)
/gene="Pif1"
/locus_tag="P301_M10566"
mRNA complement(140078..142657)
/gene="Pif1"
/locus_tag="P301_M10566"
/product="Pif1p"
CDS complement(140078..142657)
/gene="Pif1"
/locus_tag="P301_M10566"
/note="Similar to YML061C 'DNA helicase; exists in a
nuclear form that acts as a catalytic inhibitor of
telomerase; and as a mitochondrial form involved in repair
and recombination of mitochondrial DNA; mutations affect
zinc and iron homeostasis'"
/codon_start=1
/product="Pif1p"
/protein_id="EWG89252.1"
/translation="MPKWIRSTLNHIIPRRPFICSFNSFLLLKNVSHAKLSFSMSSRG
FRSNNFIQAQLKHPSILSKEDLDLLSDSDDWEEPDCIQLETEKQEKKIITDIHKEGPV
DKKPMRDKNVMNFINKDSPLSWNDMFKPSIIQPPQLISENSFDQSSQKKSRSTGFKNP
LRPALKKESSFDELQNNSISQERSLEMINENEKKKMQFGEKIAVLTQRPSFTELQNDQ
DDSNLNPHNGVKVKIPICLSKEQESIIKLAENGHNIFYTGSAGTGKSILLREMIKVLK
GIYGRENVAVTASTGLAACNIGGITIHSFAGIGLGKGDADKLYKKVRRSRKHLRRWEN
IGALVVDEISMLDAELLDKLDFIARKIRKNHQPFGGIQLIFCGDFFQLPPVSKDPNRP
TKFAFESKAWKEGVKMTIMLQKVFRQRGDVKFIDMLNRMRLGNIDDETEREFKKLSRP
LPDDEIIPAELYSTRMEVERANNSRLSKLPGQVHIFNAIDGGALEDEELKERLLQNFL
APKELHLKVGAQVMMVKNLDATLVNGSLGKVIEFMDPETYFCYEALTNDPSMPPEKLE
TWAENPSKLKAAMEREQSDGEESAVASRKSSVKEGFAKSDIGEPVSPLDSSVFDFMKR
VKTDDEVVLENIKRKEQLMQTIHQNSAGKRRLPLVRFKASDMSTRMVLVEPEDWAIED
ENEKPLVSRVQLPLMLAWSLSIHKSQGQTLPKVKVDLRRVFEKGQAYVALSRAVSREG
LQVLNFDRTRIKAHQKVIDFYLTLSSAESAYKQLEADEQVKKRKLDYAPGPKYKAKSK
SKSNSPAPISATTQSNNGIAAMLQRHSRKRFQLKKESNSNQVHSLVSDEPRGQDTEDH
ILE"
gene 142996..144126
/gene="Ogg1"
/locus_tag="P301_M10571"
mRNA 142996..144126
/gene="Ogg1"
/locus_tag="P301_M10571"
/product="Ogg1p"
CDS 142996..144126
/gene="Ogg1"
/locus_tag="P301_M10571"
/note="Similar to YML060W Mitochondrial glycosylase/lyase
that specifically excises 7,8-dihydro-8-oxoguanine
residues located opposite cytosine or thymine residues in
DNA, repairs oxidative damage to mitochondrial DNA,
contributes to UVA resistance"
/codon_start=1
/product="Ogg1p"
/protein_id="EWG89253.1"
/translation="MSYKFGKLAINKSELCLANVLQAGQSFRWIWDEKLNQYSTTMKI
GQQEKYSVVILRQDEENEILEFVAVGDCGNQDALKTHLMKYFRLDVSLKHLFDNVWIP
SDKAFAKLSPQGIRILAQEPWETLISFICSSNNNISRITRMCNSLCSNFGNLITTIDG
VAYHSFPTSEELTSRATEAKLRELGFGYRAKYIIETARKLVNDKAEANITSDTTYLQS
ICKDAQYEDVREHLMSYNGVGPKVADCVCLMGLHMDGIVPVDVHVSRIAKRDYQISAN
KNHLKELRTKYNALPISRKKINLELDHIRLMLFKKWGSYAGWAQGVLFSKEIGGTSGS
TTTGTIKKRKWDMIKETEAIVTKQMKLKVELSDLHIKEAKID"
gene complement(144344..149383)
/gene="Nte1"
/locus_tag="P301_M10576"
mRNA complement(144344..149383)
/gene="Nte1"
/locus_tag="P301_M10576"
/product="Nte1p"
CDS complement(144344..149383)
/gene="Nte1"
/locus_tag="P301_M10576"
/note="Similar to YML059C 'Serine esterase, homolog of
human neuropathy target esterase (NTE); Nte1p-mediated
phosphatidylcholine turnover influences transcription
factor Opi1p localization, affecting transcriptional
regulation of phospholipid biosynthesis genes'"
/codon_start=1
/product="Nte1p"
/protein_id="EWG89254.1"
/translation="MRSMNCTTNNTNNTGQNTKNSLGSSFNSSNYTSYRFQTCLTDQI
ISEAQTWSLSSLFNFSWVVSYFVMGASRMIFRYGWYLATLSLLRIPKWIFFKLHHVQF
TLSFWLILFALAVIVFVTYTIMKERILSQYKRLTPEFLPLENTGKSGSSANINAASTQ
SANAPPAIGSSTTGASSIIDSKKHSLKDGNENETFLSSYLDQFLSAIKIFGYLEKPVF
HDLTKNMKTQKMDEGEILLLDSTIGFAIVVEGTLQLYHEVDHSDKDHGDETDHSDTDG
LDDQDRDEEDEEEDDDIDNYDTKSCSSNLIDEEDESVGYIHLKNGLGNFQLLNTVKPG
NPLTSLVSILNLFTHSMSSYGNSNFPSELSSPIDTTVSVNNMFCSSEQNFSNTDSMTN
STNSFPTFPSSMPKLVARAATDCTIGIIPPQSFAKLTAKYPRSASHIIQMVLTKLYHV
TFQTAHDYLGLTKEIMDIEVLLNKSIVYELPYYLKEAVIRKFKTVDKSSGSADLEPKP
KNSNASSKLKKPPKAKPSDGIIQSLKIANANANTSSNSLSLKPEFTHHPSSRHVVLGS
RDQFNPGDLLSNVPLSRTMDILSPNPIHNNNRNKSNGINTSTSNQHKRSSRSSSNNAS
VHSKKFSSLSPELRNAQLSTSPLSLDNTSVHDHIHPSPVHLKGRVSPRPNLLPTTSFS
AAQEETEDSALRMALVEAMLTYLGVNKSNMSVSSSSIANMSSLNSPQLNEMYSRRPSN
ASFLMSPHCTPSDISVASSFASPQTQPTMLRILPKEYTISNKRHNKSKSQDKKKPRAY
KEELTPNLDFEDVKKDFAQGIQLKFFKKGTTIVEQNARGKGLFYIISGKVNVTTNSSS
SVVSSMSKPEQVSAQSSHKGENPHHTQHLLYSVGSGGIVGYLSSLIGYKSFVNIVAKS
DVYVGFLSSATLERLFDKYFLIYLRISDSLTKLLSSRLLKLDHALEWVHLRASETLFS
QGDSANGIYVVLNGRLRQLQQQSLSNSNTSSEEVETQNIILGELAQGESFGEVEVLTA
MNRYSTIVAVRDSELARIPRTLFELLALEHPSIMIRVSRLVAKKIVGDRTVPALTGDP
LSIKENDFTSLIPPTKASYSSSLSHKPQNITSGTITFRTITILPITSGLPVEAFAMKL
VQAFKQVGRTTIGLNQRTTLTHLGRHAFDRLSKLKQSGYFAELEEMYQTVVYISDTPV
KSNWTRTCIAQGDCILLLADARSPSAEIGEYEKLLLNSKTTARTELILLHPERYVEPG
LTHKWLRYRPWVHSHHHIQFSLTGTTLMNEGKMHVLNNGALALMDKLIQTEFSRKTQQ
NISKLLPDSIKNTVENFSSRFMKSKRQYYTPVHRHKNDFLRLARILSGQAIGLVLGGG
GARGISHLGVIQAIEEQGIPVDVIGGTSIGSFVGGLYAKDYDLVPIYGRVKKFAGRIS
SIWRMLTDLTWPVTSYTTGHEFNRGIWKTFGDTRIEDFWIQYYCNSTNITDSVQEIHS
FGYAWRYIRASMSLAGLLPPLEENGSMLLDGGYVDNLPVTEMRARGCQTIFAVDVGSA
DDRTPMEYGDSLNGFWIIFNRWNPFSSHPNIPNMAEIQVRLGYVASVNALEKAKNTPG
VVYVRPPIEEYATLDFSKFEEIYHVGVDYGRIFLQGLIDDDKMPYIPGSQETTLNSQV
PEFLLHRRNSI"
gene 149879..150085
/gene="Hug1"
/locus_tag="P301_M10581"
mRNA 149879..150085
/gene="Hug1"
/locus_tag="P301_M10581"
/product="Hug1p"
CDS 149879..150085
/gene="Hug1"
/locus_tag="P301_M10581"
/note="Similar to YML058W-A no_gene_description"
/codon_start=1
/product="Hug1p"
/protein_id="EWG89255.1"
/translation="MTMDQGLNPKQFFLDDVVLQDTLCSMSNRVNKSVKTGYLFPKDH
VPSANIIAVERRGGLSDIGKNTSN"
gene 150501..150815
/gene="Sml1"
/locus_tag="P301_M10591"
mRNA 150501..150815
/gene="Sml1"
/locus_tag="P301_M10591"
/product="Sml1p"
CDS 150501..150815
/gene="Sml1"
/locus_tag="P301_M10591"
/note="Similar to YML058W 'Ribonucleotide reductase
inhibitor involved in regulating dNTP production;
regulated by Mec1p and Rad53p during DNA damage and S
phase'"
/codon_start=1
/product="Sml1p"
/protein_id="EWG89256.1"
/translation="MQNSQDYFYAQNRCQQQQAPSTLRTVTMAEFRRVPLPPMAEVPM
LSTQNSMGSSASASASSLEMWEKDLEERLNSIDHDMNNNKFGSGELKSMFNQGKVEEM
DF"
gene complement(151153..151530)
/locus_tag="P301_M10596"
mRNA complement(151153..151530)
/locus_tag="P301_M10596"
/product="hypothetical protein"
CDS complement(151153..151530)
/locus_tag="P301_M10596"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89257.1"
/translation="MAGTLFIILRFVDTTLPSSRVYCVRSLEVSVAVELAAATVLAFE
SIGVVDDCGRSVLFSIILIAAFICSVFLIASEDIAGSRRSTGSCVTLWEGRNISFCLY
RSNWLNTVPVGYMFFLRKNRSLD"
gene 151298..153112
/gene="Cmp2"
/locus_tag="P301_M10601"
mRNA 151298..153112
/gene="Cmp2"
/locus_tag="P301_M10601"
/product="Cmp2p"
CDS 151298..153112
/gene="Cmp2"
/locus_tag="P301_M10601"
/note="Similar to YML057W 'Calcineurin A; one isoform (the
other is CNA1) of the catalytic subunit of calcineurin, a
Ca++/calmodulin-regulated protein phosphatase which
regulates Crz1p (a stress-response transcription factor),
the other calcineurin subunit is CNB1'"
/codon_start=1
/product="Cmp2p"
/protein_id="EWG89258.1"
/translation="MSSDAIRNTEQINAAIKIIENKTERPQSSTTPIDSKASTVAAAN
STATETSRDLTQYTLDDGRVVSTNRRIMNKVPAITSHVPTDEELFQPNGIPRHEFLRD
HFKREGKLSAAQAARIVTLATELFSKEPNLISVPAPITVCGDIHGQYFDLLKLFEVGG
DPATTSYLFLGDYVDRGSFSFECLIYLYSLKLNFNDHFWLLRGNHECKHLTSYFTFKN
EMLHKYNLDIYEKCCESFNNLPLAALMNGQYLCVHGGISPELNSLQDINNLKRFREIP
SHGLMCDLLWADPIEEYDEVLDKDLTEEDIVNSKTMVPHHGKMAPSRDMFVPNSVRGC
SYAFTYRAACHFLQETGLLSIIRAHEAQDAGYRMYKNTKTLGFPSLLTLFSAPNYLDT
YNNKAAILKYENNVMNIRQFNMTPHPYWLPDFMDVFTWSLPFVGEKVTEMLVAILNIC
TEDELENDTPVIEELVGTDKKLPQAGKSEATPQPATSASPKHASILDDEHRRKALRNK
ILAVAKVSRMYSVLREETNKVQFLKDHNSGVLPRGALSNGVKGLDEALSTFERARKHD
LINEKLPPSLDELKKENKKYYEKVWQKVHEHDAKNDSK"
gene complement(153312..155295)
/gene="Imd4"
/locus_tag="P301_M10606"
mRNA complement(join(153312..154426,154836..155295))
/gene="Imd4"
/locus_tag="P301_M10606"
/product="Imd4p"
CDS complement(join(153312..154426,154836..155295))
/gene="Imd4"
/locus_tag="P301_M10606"
/note="Similar to YML056C Inosine monophosphate
dehydrogenase, catalyzes the first step of GMP
biosynthesis, member of a four-gene family in S.
cerevisiae, constitutively expressed"
/codon_start=1
/product="Imd4p"
/protein_id="EWG89259.1"
/translation="MSAAPLDYKKALEHLKTYSSKDGLSVQELMDSTTRGGLTYNDFL
VLPGLVNFPSSAVSLQTKLTKKITLNTPFVSSPMDTVTEADMAIYMALLGGIGFIHHN
CTPKEQASMVKKVKMFENGFINSPIVISPTTTVGEVKVMKRKFGFSGFPVTEDGKCPG
KLVGLVTSRDIQFLEDDSLVVSEVMTKNPVTGIKGITLKEGNEILKQTKKGKLLIVDD
NGNLVSMLSRADLMKNQNYPLASKSATTKQLLCGAAIGTIEADKERLRLLVEAGLDVV
ILDSSQGNSVFELNMLKWVKESFPGLEVIAGNVVTREQAANLIAAGADGLRIGMGTGS
ICITQEVMACGRPQGTAVYNVCQFANQFDVPCMADGGVQNIGHITKALALGSSTVMMG
GMLAGTTESPGEYFYKDGKRLKAYRGMGSIDAMQKTGNKGNASTSRYFSESDSVLVAQ
GVSGAVVDKGSIKKFIPYLYNGLQHSCQDIGCESLTLLKENVQNGEVRFEFRTASAQL
EGGVHNLHSYEKRLYN"
gene complement(154654..154739)
/locus_tag="P301_M10611"
ncRNA complement(154654..154739)
/ncRNA_class="snoRNA"
/locus_tag="P301_M10611"
/product="SNR54"
gene 155904..156443
/gene="Spc2"
/locus_tag="P301_M10626"
mRNA 155904..156443
/gene="Spc2"
/locus_tag="P301_M10626"
/product="Spc2p"
CDS 155904..156443
/gene="Spc2"
/locus_tag="P301_M10626"
/note="Similar to YML055W 'Subunit of signal peptidase
complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes
cleavage of N-terminal signal sequences of proteins
targeted to the secretory pathway; homologous to mammalian
SPC25'"
/codon_start=1
/product="Spc2p"
/protein_id="EWG89260.1"
/translation="MSSAKPINVYSIPELNQALDEALPSVFARLNYERSYALLDAKLY
IGYSIAVVAGLSFFLDKKFERDQIVTYQKLLVGAYFVLSLLFWYFSRFIEKGTVYVGK
RRGTKEEIYVKTKFEKNEPLYLVELVQKKKGENSKKELKAKLEVNKVFNESGYLQNDA
YFKWFSEQHNVLDTKKMNK"
assembly_gap 156532..156563
/estimated_length=32
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(156641..158416)
/gene="Cyb2"
/locus_tag="P301_M10631"
mRNA complement(156641..158416)
/gene="Cyb2"
/locus_tag="P301_M10631"
/product="Cyb2p"
CDS complement(156641..158416)
/gene="Cyb2"
/locus_tag="P301_M10631"
/note="Similar to YML054C 'Cytochrome b2 (L-lactate
cytochrome-c oxidoreductase), component of the
mitochondrial intermembrane space, required for lactate
utilization; expression is repressed by glucose and
anaerobic conditions'"
/codon_start=1
/product="Cyb2p"
/protein_id="EWG89261.1"
/translation="MLKYKPLLKISKNCEAAILRASKTRLNTIRAYGSTVPKSKSFEQ
DSRKRTQSWTALRVGAILAATSSVAYLNWHNGQIDNEPKLDMNKQKISPAEVAKHNKP
DDCWVVINGYVYDLTRFLPNHPGGQDVIKFNAGKDVTAIFEPLHAPNVIDKYIAPEKK
LGPLQGSMPPELVCPPYAPGETKEDIARKEQLKSLLPPLDNIINLYDFEYLASQTLTK
QAWAYYSSGANDEVTHRENHNAYHRIFFKPKILVDVRKVDISTDMLGSHVDVPFYVSA
TALCKLGNPLEGEKDVARGCGQGVTKVPQMISTLASCSPEEIIEAAPSDKQIQWYQLY
VNSDRKITDDLVKNVEKLGVKALFVTVDAPSLGQREKDMKLKFSNTKAGPKAMKKTNV
EESQGASRALSKFIDPSLTWKDIEELKKKTKLPIVIKGVQRTEDVIKAAEIGVSGVVL
SNHGGRQLDFSRAPIEVLAETMPILEQRNLKDKLEVFVDGGVRRGTDVLKALCLGAKG
VGLGRPFLYANSCYGRNGVEKAIEILRDEIEMSMRLLGVTSIAELKPDLLDLSTLKAR
TVGVPNDVLYNEVYEGPTLTEFEDA"
gene complement(158730..158888)
/locus_tag="P301_M10646"
mRNA complement(158730..158888)
/locus_tag="P301_M10646"
/product="hypothetical protein"
CDS complement(158730..158888)
/locus_tag="P301_M10646"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89262.1"
/translation="MIPFPAQHEIFHAYIGRITPHSSRCIANMWHSAHFFHENSLSIM
KTLVPWTL"
gene 159566..159654
/locus_tag="P301_M10651"
tRNA 159566..159654
/locus_tag="P301_M10651"
/product="tRNA-Tyr"
/note="TY(GUA)M1"
gene complement(159889..160518)
/locus_tag="P301_M10656"
mRNA complement(159889..160518)
/locus_tag="P301_M10656"
/product="hypothetical protein"
CDS complement(159889..160518)
/locus_tag="P301_M10656"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89263.1"
/translation="MLSYYEHNTAFQTNNCNSGSNAATTYNSDANNDTIMNKRKNDHF
EFDTHTFYQRSKRTKRDSVSTKFSVGSGCANLNNNNNNIIINSNNNNNNNHNHNNSNN
TATYNNIHYQKNIEICPLKPVSMHHTMNSRLLNESEFYSETEEYMIHGYFGNTNRDIT
GTSPTGSASIIQHQYHLLPSQSIIASQAPGTAMAALTNNNIANDYMDID"
gene 161165..162073
/gene="Sur7"
/locus_tag="P301_M10666"
mRNA 161165..162073
/gene="Sur7"
/locus_tag="P301_M10666"
/product="Sur7p"
CDS 161165..162073
/gene="Sur7"
/locus_tag="P301_M10666"
/note="Similar to YML052W 'Plasma membrane protein that
localizes to furrow-like invaginations (MCC patches);
component of eisosomes; associated with endocytosis, along
with Pil1p and Lsp1p; sporulation and plasma membrane
sphingolipid content are altered in mutants'"
/codon_start=1
/product="Sur7p"
/protein_id="EWG89264.1"
/translation="MVKVWNIVLRLVVLLFLAGNTLLLILMIISGATDHYPVNRFYWV
QGNTTGIPNAGDETRWTFWGACLQDKDGSDTCTSNLAPAYPISPVDNFNTHINVPHQF
ISKRDAFYYLTRFSFCFFWIALAFVGVSFILYVLTWCSKMLSEMVLILMSFGFVFNTA
AVVLQTAASAMAKNAFHDDHRSAQLGASMMGMAWASVFLCIVEFILLVFWSVRARLAS
TYSIDNSRYRTSSRWNPFHREKEQATDPILTATGPEDMQQSASIVGPSSNANPVTATA
ATENQPKGINFFTIRKSHERPDDVSV"
gene 162356..163663
/gene="Gal80"
/locus_tag="P301_M10671"
mRNA 162356..163663
/gene="Gal80"
/locus_tag="P301_M10671"
/product="Gal80p"
CDS 162356..163663
/gene="Gal80"
/locus_tag="P301_M10671"
/note="Similar to YML051W 'Transcriptional regulator
involved in the repression of GAL genes in the absence of
galactose; inhibits transcriptional activation by Gal4p;
inhibition relieved by Gal3p or Gal1p binding'"
/codon_start=1
/product="Gal80p"
/protein_id="EWG89265.1"
/translation="MDYNKRSSVSTVPNAAPIRVGFVGLNAAKGWAIKTHYPAILQLS
SQFQITALYSPKIETSIATIQRLKLSNATAFPTLESFASSSTIDMIVIAIQVASHYEV
VMPLLEFSKNNPNLKYLFVEWALACSLDQAESIYKAAAERGVQTIISLQGRKSPYILR
AKELISQGYIGDINSIEIAGNGGWYGYERPVKSPKYIYEIGNGVDLVTTTFGHTIDIL
QYMTSSYFSRINAMVFNNIPEQELIDERGNRLGQRVPKTVPDHLLFQGTLLNGNVPVS
CSFKGGKPTKKFTKNLVIDIHGTKGDLKLEGDAGFAEISNLVLYYSGTRANDFPLANG
QQAPLDPGYDAGKEIMEVYHLRNYNAIVGNIHRLYQSISDFHFNTKKIPELPSQFVMQ
GFDFEGFPTLMDALILHRLIESVYKSNMMGSTLNVSNISHYSL"
gene 164337..>164821
/gene="Aim32"
/locus_tag="P301_M10681"
mRNA 164337..>164821
/gene="Aim32"
/locus_tag="P301_M10681"
/product="Aim32p"
CDS 164337..>164821
/gene="Aim32"
/locus_tag="P301_M10681"
/note="Similar to YML050W 'Putative protein of unknown
function; null mutant is viable and displays elevated
frequency of mitochondrial genome loss'"
/codon_start=1
/product="Aim32p"
/protein_id="EWG89266.1"
/translation="MKLYVIKETDIEEFASFLDEGAIQAPKLSFQDYLSGKAKASQQV
HEVHHRKLTRFQGETFLRDWNLVCGHYKRDAKCGEMGPDIIAAFQDEKLFPENNLALI
SHIGGHIFAGNVIFYKLFGREKMQNKLDSLWFGKVYPHNLKLLCRKLGKRKIIDEMYR
GG"
assembly_gap 164822..165048
/estimated_length=227
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<165049..169260)
/gene="Rse1"
/locus_tag="P301_M10686"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 169381..170592
/gene="Gsf2"
/locus_tag="P301_M10691"
mRNA 169381..170592
/gene="Gsf2"
/locus_tag="P301_M10691"
/product="Gsf2p"
CDS 169381..170592
/gene="Gsf2"
/locus_tag="P301_M10691"
/note="Similar to YML048W 'ER localized integral membrane
protein that may promote secretion of certain hexose
transporters, including Gal2p; involved in
glucose-dependent repression'"
/codon_start=1
/product="Gsf2p"
/protein_id="EWG89267.1"
/translation="MEIYIRLNADVEHDYAFQVSNEDTINNKIKKIFPSKTGLADLMV
LRPSIFHEKEPVKFYKSIHPGYLSEGGCLMFHYEADNEENLEELNDSKPLIDQLWPGQ
LVVPEWKLSKKNIWVYTIIMLAWLYTDLPDAISPTPGICLTNQLSRLLIPVAKHMDLP
EIAAKLEQEVQANYSSLVAQWLFFVMHIFKVGIITLFLKLGIANPISFNPYKLWSLRD
LTSPSANGAKNSGGNNNTTDLKTRLRSLGWIGAKRATYDDYQTNYYNYVIDKMGGAVA
AYRAGAIRKAAAPGIQLVAGEGFQSPLEDRFTASTFTAIKTERKFILSEEYFVELENN
LKKILEEYDGDIGKMNAEIRRFRRFGIYEPDEKLASLVKLRREIADEKEKASNSDATF
GIKKNDLKKSN"
gene 170867..171217
/locus_tag="P301_M10696"
mRNA 170867..171217
/locus_tag="P301_M10696"
/product="hypothetical protein"
CDS 170867..171217
/locus_tag="P301_M10696"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89268.1"
/translation="MVFVTIIIIINIXXXXXXXXXXNEDRFTFNAFLQSKKLRLHLYV
FMFVFLGEICLYGRIYSFVRFYIRVTVVTRYCTFVAIIRTIIIEFFLRFVVTTYGPFF
LIIYQYSQFVDSST"
assembly_gap 170904..170932
/estimated_length=29
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(170990..172048)
/gene="Prm6"
/locus_tag="P301_M10701"
mRNA complement(170990..172048)
/gene="Prm6"
/locus_tag="P301_M10701"
/product="Prm6p"
CDS complement(170990..172048)
/gene="Prm6"
/locus_tag="P301_M10701"
/note="Similar to YML047C 'Pheromone-regulated protein,
predicted to have 2 transmembrane segments; regulated by
Ste12p during mating'"
/codon_start=1
/product="Prm6p"
/protein_id="EWG89269.1"
/translation="MESSLQKLKFQDIDINLIPTAKWTTKLQYILYTWCQSILHVAMF
FSDIYTCIKLLAFNTWSNNIIQPFLEFRISKWLFSGCILCSSLILIWELVIGLRVYRK
KEITSNYMNGISRLINCLFNFKKYQIFELIVLTDEKKFSKWLFFSYFEISGCLRLLFG
DSPRQIINGLTLWSVLLTVSNETSSGTHSTQSLGNLDDLNGIINKIKHIAKTNYEESV
ILSFMLFSFIIWVILISKLILSIIIFIIFIRPRFLSSKRNVKGYELKLRKYVSKVIDE
NLSRTVYELGILIDDEEEGTICGDNKTQKKFDYDSPDYGDESTIPSYYCYSDVETYER
VYTPIKAYFPQKYKHKYI"
gene 172448..174337
/gene="Prp39"
/locus_tag="P301_M10706"
mRNA 172448..174337
/gene="Prp39"
/locus_tag="P301_M10706"
/product="Prp39p"
CDS 172448..174337
/gene="Prp39"
/locus_tag="P301_M10706"
/note="Similar to YML046W U1 snRNP protein involved in
splicing, contains multiple tetriatricopeptide repeats"
/codon_start=1
/product="Prp39p"
/protein_id="EWG89270.1"
/translation="MPDETNFTIEDIEPRPDALRGLDTQFLHDNTALVQAYRGLDWSD
ISSLTQMVDVIEQTVVKYGNPNDSIKLALETILWQILRKYPLLFGFWKRFATIEYQLF
GLKKSIAVLATSVKWFPTSLELWCDYLNVLCVNNPNETDFIRNNFEIAKDLIGKQFLS
HPFWDKFIEFEVGQKNWHNVQRIYEYIIEVPLHQYARFFTSYKKFLNEKNLKTTRNID
IILRKTQTTVNEIWQFESKIKQPFFNLGQVLNDDLENWSRYLKFVTDPSKSLDKEFVM
SVFDRCLIPCLYHENTWMMYIKWLTKKNISDEVVVDIYQKANTFLPLDFKTLRYDFLR
FLKRKYRSNNTLFNNIFNETVSRYLKIWPNDILLMTEYLCMLKRHSFKNSLDQSPKEI
LEKQTSFTKILETSISNYINNQIDAKVHLQTLINDKNLSIVVVELIKTTWLVLKNNMQ
TRKYFNLYQKNILIKNSVPFWLTYYKFEKSNVNFTKLNKFIRELGVEIYLPTTVMNDI
LTDYKTFYLTHSNIVTYESSIIDSNTFDPILYPELKMSNPKYDPVLNTTANVDWHKKT
EWKEAGHIGITTERPQISNSIIECNSGTLIQKPISLPNFRNLEKINQVKMNDLYTEEF
LKEGK"
gene complement(174871..174941)
/locus_tag="P301_M10711"
tRNA complement(174871..174941)
/locus_tag="P301_M10711"
/product="tRNA-Gly"
/note="TG(GCC)M"
assembly_gap 174958..174989
/estimated_length=32
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(175361..177091)
/gene="Rrn11"
/locus_tag="P301_M10716"
mRNA complement(175361..177091)
/gene="Rrn11"
/locus_tag="P301_M10716"
/product="Rrn11p"
CDS complement(175361..177091)
/gene="Rrn11"
/locus_tag="P301_M10716"
/note="Similar to YML043C 'Component of the core factor
(CF) rDNA transcription factor complex; CF is required for
transcription of 35S rRNA genes by RNA polymerase I and is
composed of Rrn6p, Rrn7p, and Rrn11p'"
/codon_start=1
/product="Rrn11p"
/protein_id="EWG89271.1"
/translation="MDHNKHVISSHLXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXYINYISRRFDR
ISKKSTTTDSLPTPENSAAENNDEEEGQNSEAGAYRRSVLQQKKRRRERHWRSVVGEI
YSTTESETDSQEEEAEEGGEHDTGIDKEDSDEERKFWKKYEKPEKSFEIWRTVSSQNK
QPLNKQKMTYHNFKKIEKIPLRKMEIPLLHCTKENKLYFQSISRGLEPLKTSTSEVRN
YRTRHIVTLTDLLHLNVSRHNWSLAYKIFATLIRIPGVQIKSLWGIGVEILDNLSNSS
SGLDFLQWMCQIYSSKSRFVQNINYRSIVPPFQTGSRTHTAKFAITYLWSSLINCQKS
MEPSPNIIDKPFDTENDLLQELIDKISEWVLTPPFMEDAEVWFIYASCHLLKADTLSR
QFVNDNKNNDLIGLDRDIKINQVIKHIHYVRTFLKICLDKGGFAVPSRLIENQLKSFE
SRLYGEAQDIQERDVANVYDSIDNSSVENSFGDVYETNAEFLDTQLMDLSPEDNGLDE
MHYSDEDSSE"
assembly_gap 176821..177055
/estimated_length=235
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 177913..179925
/gene="Cat2"
/locus_tag="P301_M10721"
mRNA 177913..179925
/gene="Cat2"
/locus_tag="P301_M10721"
/product="Cat2p"
CDS 177913..179925
/gene="Cat2"
/locus_tag="P301_M10721"
/note="Similar to YML042W Carnitine acetyl-CoA transferase
present in both mitochondria and peroxisomes, transfers
activated acetyl groups to carnitine to form
acetylcarnitine which can be shuttled across membranes"
/codon_start=1
/product="Cat2p"
/protein_id="EWG89272.1"
/translation="MRICHSRTLSNLKDLPITSRRAMHSAIVNYSTQKAQFPVETNNG
EHYWAEKPNKFYQNKRPDFQGITFAKQQELPSLPVPELKSTLDKYLQTIRPFCNDVET
FERQQLLCKDFSEHMGPILQDRLKEYANDKRNWMAKFWDEQSYLQYNDPIVPYVSYFY
SHMPLPNHLSKIDNDPLIKATAIISTVVKFIEAIKDESLPVEIIKGMPFCMNSFSLMF
NTSRLPGKPEDNQDTNIFYSVYENNFVTIAYKGKFYKLMTHDGNDKPLSENEIWRQLY
SVVFQGSQSDPKLGGIGSLTSLPRDQWREVHMELMKDPISQDSLETIHKSSFMLCLDL
DQSPVTLEEKSRNCWHGDGINRFYDKSLQFLVTGNGSSGFLAEHSKMDGTPTLFLNNY
VCRQLNKLDVDDFMRKVITPSSTVAMKPMELPFIITPKIHKAIESAQLQFKETIGEHD
LRVWHYNKYGKTFIKRHGMSPDAFIQQVIQLAVFKYLKRQLPTYEAASTRKYFKGRTE
TGRSVSTASLEFVSKWQNGDVPIAEKIQALKHSAKEHSTYLRNAANGNGVDRHFFGLK
NMLKSNDDQIPPLFKDPLFNYSSTWLISTSQLSSEYFDGYGWSQVNDNGFGLAYMLNN
EWLHINIVNKLAKSGASVNRLHYYLSQAADEIFDALENENKRKAKL"
gene complement(180038..180880)
/gene="Vps71"
/locus_tag="P301_M10731"
mRNA complement(180038..180880)
/gene="Vps71"
/locus_tag="P301_M10731"
/product="Vps71p"
CDS complement(180038..180880)
/gene="Vps71"
/locus_tag="P301_M10731"
/note="Similar to YML041C 'Nucleosome-binding component of
the SWR1 complex, which exchanges histone variant H2AZ
(Htz1p) for chromatin-bound histone H2A; required for
vacuolar protein sorting'"
/codon_start=1
/product="Vps71p"
/protein_id="EWG89273.1"
/translation="MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSR
PVKRINYSLADLEARLYTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRF
MELNTENFSDLPNVPTLLSDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSK
YGHGWVLKPETLREIQLSYKSTKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLN
LMDKNVIYHNVYNKRYFKVLPLITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETR
CRNR"
gene complement(181194..181296)
/locus_tag="P301_M10736"
tRNA complement(181194..181296)
/locus_tag="P301_M10736"
/product="tRNA-Pro"
/note="TP(UGG)M"
gene complement(182123..183451)
/gene="Ymd8"
/locus_tag="P301_M10741"
mRNA complement(182123..183451)
/gene="Ymd8"
/locus_tag="P301_M10741"
/product="Ymd8p"
CDS complement(182123..183451)
/gene="Ymd8"
/locus_tag="P301_M10741"
/note="Similar to YML038C Putative nucleotide sugar
transporter, has similarity to Vrg4p"
/codon_start=1
/product="Ymd8p"
/protein_id="EWG89274.1"
/translation="MNRTVFLAFVFGWYFCSIALSIYNRWMFDPKDGLGIGYPVLVTT
FHQATLWLLSGIYIKLRHKPVKNVLRKNNGFNWSFFLKFLLPTAVASAGDIGLSNVSF
QYVPLTIYTIIKSSSIAFVLLFGCIFKLEKFHWKLALSVIIMFVGVALMVFKPSDSTS
TKNDQALVIFGSFLVLASSCLSGLRWVYTQLMLRNNPIQTNTAAAVEESDGALFTENE
DNVDNEPVVNLANNKMLENFGESKPHPIHTIHQLAPIMGITLLLTSLLVEKPFPGIFS
SSIFRLDTSNGGVGTETTVLSIVRGIVLLILPGFAVFLLTICEFSILEQTPVLTVSIM
GIVKELLTVIFGIIILSERLSGFYNWLGMLIIMADVCYYNYFRYKQDLLQKYHSVSTQ
DNRNELKGFQDFEQLGSKKIAPYSISVDLTNQEYELDMIAQNVSRSSQQV"
gene complement(183734..184756)
/locus_tag="P301_M10746"
mRNA complement(183734..184756)
/locus_tag="P301_M10746"
/product="hypothetical protein"
CDS complement(183734..184756)
/locus_tag="P301_M10746"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89275.1"
/translation="MDENKIIDQLFSKEYTPQDDSEQAKNGDVSLYGLLDEVANGRRL
MNCLFHSPMQMGNKLSTDKLDGKCRQIQRDWIDEEKTITMNSGALQLDGPVLFSWSHN
VAPTSHQETINTTFKQGSPSRGSNKPKITTTSQLFDRASAEIDKCIKPNSKSWMVEER
FERNEAHTADGKKPSTWANSDFKVDPLQKFVVKELPKEKKKSDGDKTKKNKSKRKSFF
GFWGHSGSKSGSKKKSEKPIEAKNEIQDEVSQKSGLSPDDDTTFSDKNTIQSKQESMS
DQQAEPKVHEPAVTNTGCSEHDDGDGFEQVPAQSSYHPSSEPSIASTPSLTLDSFIPL
QPKKKI"
gene 184990..185641
/gene="Cgi121"
/locus_tag="P301_M10751"
mRNA join(184990..185445,185552..185641)
/gene="Cgi121"
/locus_tag="P301_M10751"
/product="Cgi121p"
CDS join(184990..185445,185552..185641)
/gene="Cgi121"
/locus_tag="P301_M10751"
/note="Similar to YML036W 'Component of the EKC/KEOPS
complex with Bud32p, Kae1p, Pcc1p, and Gon7p; EKC/KEOPS
complex is required for t6A tRNA modification and may have
roles in telomere maintenance and transcription; Cgi121p
is dispensable for tRNA modification'"
/codon_start=1
/product="Cgi121p"
/protein_id="EWG89276.1"
/translation="MVVSIIPQFPDIKVSLALFEQVKNAKEIRSKMSELSTSFAFIDP
RLVCSGEQMYSAIYKTLIEVKYNKMRTRNLNSECVLCLSPTSNISDAFLKFGIKDDSS
QLICLKFHTNTDDVDKEQLRTIMTSIVKGQEIEFNDDNLSRFYDEALIRKIYKLSDDF
KPQDVNGLSRALVDAIQLRGV"
gene complement(185777..188209)
/gene="Amd1"
/locus_tag="P301_M10756"
mRNA complement(185777..188209)
/gene="Amd1"
/locus_tag="P301_M10756"
/product="Amd1p"
CDS complement(185777..188209)
/gene="Amd1"
/locus_tag="P301_M10756"
/note="Similar to YML035C 'AMP deaminase, tetrameric
enzyme that catalyzes the deamination of AMP to form IMP
and ammonia; may be involved in regulation of
intracellular adenine nucleotide pools'"
/codon_start=1
/product="Amd1p"
/protein_id="EWG89277.1"
/translation="MDNQATQRLNDLSLEPAPSHDEQDGSGLVIDIDQRKIGDEQAGV
VVDDETPPLEQQDSHESLAADSRNANFSYHENQQLLENGTKQLALDEHDSHSAILEQP
SHSTNCSSSNIAAMNKGHDSADHASQNSGGKPRTLSASAQHILPETLKSFAGAPVVNK
QVRTSASYKMGMLADDASQQFLDDPSSELIDLYSKVAECRNLRAKYQTISVQNDDQNP
KNKPGWVVYPPPPKPSYNSDTKTVVPVTNKPDAEVFDFTKCEIPGEDPDWEFTLNDDD
SYVVHRSGKTDELIAQIPTLRDYYLDLEKMISISSDGPAKSFAYRRLQYLEARWNLYY
LLNEYQETSVSKRNPHRDFYNVRKVDTHVHHSACMNQKHLLRFIKHKLRHSKDEKVIF
RDGKLLTLDEVFRSLHLTGYDLSIDTLDMHAHKDTFHRFDKFNLKYNPIGESRLREIF
LKTNNYIKGTYLADITKQVIFDLENSKYQNCEYRISVYGRSLDEWDKLASWVIDNKVI
SHNVRWLVQIPRLYDIYKKTGIVQSFQDICKNLFQPLFEVTKNPQSHPKLHVFLQRVI
GFDSVDDESKVDRRFHRKYPKPSLWEAPQNPPYSYYLYYLYSNVASLNQWRAKRGFNT
LVLRPHCGEAGDPEHLVSAYLLAHGISHGILLRKVPFVQYLYYLDQVGIAMSPLSNNA
LFLTYDKNPFPRYFKRGLNVSLSTDDPLQFSYTREPLIEEYSVAAQIYKLSNVDMCEL
ARNSVLQSGWEAQIKKHWIGKDFDKSGVEGNDVVRTNVPDIRINYRYDTLSTELELVN
HFANFKRTIEEK"
gene complement(188778..189176)
/locus_tag="P301_M10766"
mRNA complement(188778..189176)
/locus_tag="P301_M10766"
/product="hypothetical protein"
CDS complement(188778..189176)
/locus_tag="P301_M10766"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89278.1"
/translation="MGEADFSLRARLLLRGEAIDGEDEGRSIFTIPSSLGRTFRYIFR
NCGICGLTFSSNSPTSAFFLALDGKSTLFSTNILLKVATFIEFGSKPSSKYSKSLFIA
ALTEYCYRSTVCCSGLYVHSVWSQSTINND"
gene 188875..191505
/gene="Src1"
/locus_tag="P301_M10771"
mRNA join(188875..190794,190921..191505)
/gene="Src1"
/locus_tag="P301_M10771"
/product="Src1p"
CDS join(188875..190794,190921..191505)
/gene="Src1"
/locus_tag="P301_M10771"
/note="Similar to YML034W 'Inner nuclear membrane protein;
functions in regulation of subtelomeric genes and is
linked to TREX (transcription export) factors; SRC1
produces 2 splice variant proteins with different
functions; alternative splicing of SRC1 pre-mRNA is
promoted by Hub1p; mutant has aneuploidy tolerance'"
/codon_start=1
/product="Src1p"
/protein_id="EWG89279.1"
/translation="MNSDLEYLEDGFDPNSMKVATLRRILVENNVDFPSNARKNALVG
LFDEKVKPQIPQLRKMYLNVRPSDEGIVKMDRPSSSPSIASPRRSRRARREKSASPMA
KQFKKNRILDDVSNDDDDDDDDDDDNDKKDDPLIVPSGTDTDEVDDEEDDVITSSSNK
SDTNDFQQNSDTRKKRKDPDSDDWSESNSKENKIDNKHLNLLSSDSEIEQDYQKAKKR
KTSDLNQEHGNGSAILGKLSVKTPIKNTNRKPVSMDNFNDSLTSSGTENDPFVPNIRH
NPKELGTANGTGHSTPLSKLKVSASFADKLPQKEVPSTILVPEVEQQEPSQSERTPSL
FSSEGSGSESEAPLLPEITTPGPHQPMGNTSNNVVEMIDTDSSNLVSDEDEVLVPTRI
ETPQLPTEKDVEKCEARVQELQEEVNEQLEHENGSEFDVKQGSGKVGNRHKFKRALKF
LSKSLLALFLFCIFIVIPLLFGLWYREQRLLIGYCGHEVPSHRVSGNSFEFIQKLDNL
LQDYRPKCIPCPPNGICYPYLKLKCKPDYKLAPSRLDFLEIIPAQGKCVKDDKKQQLV
SEVVEKSLEFLRAKNAQISCGDGKDDIESGMTEDALYQIFNEARAPWIRDDEFEDLWI
QVIKDLTEEPEILWRQLSPTDNNIGGNSNNIIKTNDVPRQKRHLPEKFISKTRNFRST
SKKYIGMKCRFEREIYQTYKKFQRPIWLMFLLIVISKVIEIKLKNYYRKKARIEELVT
QTMEKLKFQKIKSMSDPKENAYLSIVQLRDIFLSDIVDLKYKNQLWSEVVKYLEHNNS
NIKSNLTEIRGEIMKCWEWIGPMELNEPKDSAENKI"
gene complement(191865..193280)
/gene="Rad52"
/locus_tag="P301_M10776"
mRNA complement(191865..193280)
/gene="Rad52"
/locus_tag="P301_M10776"
/product="Rad52p"
CDS complement(191865..193280)
/gene="Rad52"
/locus_tag="P301_M10776"
/note="Similar to YML032C 'Protein that stimulates strand
exchange by facilitating Rad51p binding to single-stranded
DNA; anneals complementary single-stranded DNA; involved
in the repair of double-strand breaks in DNA during
vegetative growth and meiosis'"
/codon_start=1
/product="Rad52p"
/protein_id="EWG89280.1"
/translation="MNEIMDMDEKKPVFGNHSEDIQTKLDKKLGPEYISKRVGFGTSR
IAYIEGWRVINLANQIFGYNGWSTEVKSVVIDFLDERQGKFSIGCTAIVRVTLTSGTY
REDIGYGTVENERRKPAAFERAKKSAVTDALKRSLRGFGNALGNCLYDKDFLAKIDKV
KFDPPDFDENNLFRPTDEISESSRTNTLHENQEQQQYPNKRRQLTKVTNTNPDSTKNL
VKIENTVSRGTPMMAAPAEANSKNSSNKDTDLKSLDASKQDQDDLLDDSLMFSDDFQD
DDLINMGNTNSNVLTTEKDPVVAKQSPTASSNPEAEQITFVTAKAATSVQNERYIGEE
SIFDPKYQAQSIRHTVDQTTSKHVPASVLKDKTMTTARDSVYEKFAPKGKQLSMKNND
KELGPHMLEGAGNQVPRETTPIKTNATAFPPAAAPRFAPPSKVVHPNGNGAVPAVPQQ
RSTRREVGRPKINPLHARKPT"
gene complement(193436..193771)
/locus_tag="P301_M10781"
mRNA complement(193436..193771)
/locus_tag="P301_M10781"
/product="hypothetical protein"
CDS complement(193436..193771)
/locus_tag="P301_M10781"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89281.1"
/translation="MIKRETRLRAKILGINANASSHNGECGNASSEITTVWIIEHMKN
NLVTKWLNRVLHTSLKMVWYVYRGFNNSLGVCIICLPCNVLSSSQSHLLIAKESALPV
SDSRCYYIL"
gene 193539..195506
/gene="Ndc1"
/locus_tag="P301_M10786"
mRNA 193539..195506
/gene="Ndc1"
/locus_tag="P301_M10786"
/product="Ndc1p"
CDS 193539..195506
/gene="Ndc1"
/locus_tag="P301_M10786"
/note="Similar to YML031W 'Nuclear envelope protein with
multiple putative transmembrane domains, required for
nuclear pore complex assembly and spindle pole body
duplication; required for meiosis II'"
/codon_start=1
/product="Ndc1p"
/protein_id="EWG89282.1"
/translation="MIQTPRELLNPRYTYHTIFSDVCKTRFNHLVTRLFFICSIIQTV
VISLLALPHSPLWELALAFIPNILALNLVSLLIIVTRKNYMHVKNFGFANSLTFILGQ
LLSVKFLVYQGVYSMGSILLSFVLGVVFGRGGSGWKPYYKLFIWLVVPTIYNLQHHVT
DADKLSFNCENFFQAPQDYVLERVKRIMEKSVILSVISMFVLPIFTTVFFSRQKSGLF
DSFTNGVLAVTNLLIISCIIFITFEFINIAFDAHMSIGCLHKGKLISNLSSTPMETLL
SGLSADKPFTRLTAYQELAYRATSLDPSLRAPIYHSKFRSSSGNTWSLILNECLKTIQ
INNEKVVQYLRSVQDLGGSATARHKKKVENLDYMYENGKLTSANERLFGNRPSMMAPL
RDNGLLDESPNRLRVRTDDSVLLNRGNKKRHRSSYYDNDLDETTQTFNGSIFTHETTF
MTAMRLMLKKLKNSIMSFIFPSYAERQSSDESDNYRLLPNGSNKAQISIIDIWSISKK
RQAEKLVPLPICHANSVVALTGLLIRSKTEDPKGGIIASVGDILKTLERSICALGEFA
DWDPESMAYTAFQTQRTAQDRVQQDSEDEDSMKDTTDMISVLYQLSTSAFMEIVLEYN
VALNDVYLDADVAKLANWFLEVYASGNPNAT"
gene 195785..196264
/gene="Aim31"
/locus_tag="P301_M10791"
mRNA 195785..196264
/gene="Aim31"
/locus_tag="P301_M10791"
/product="Aim31p"
CDS 195785..196264
/gene="Aim31"
/locus_tag="P301_M10791"
/note="Similar to YML030W 'Putative protein of unknown
function; GFP-fusion protein localizes to mitochondria;
may interact with respiratory chain complexes III or IV;
null mutant is viable and displays reduced frequency of
mitochondrial genome loss'"
/codon_start=1
/product="Aim31p"
/protein_id="EWG89283.1"
/translation="MSRMPSSFDVTERDLDDMTFGERIIYHCKKQPLVPIGCLLTTGA
VILAAQNVRLGNKWKAQYYFRWRVGLQAATLVALVAGSFIYGTSGKELKAKEEQLKEK
AKMREKLWIQELERREEETEARRKRAELARMKTLENEEEIKNLEKELSDLENKLGKK"
gene 196714..199230
/gene="Usa1"
/locus_tag="P301_M10796"
mRNA 196714..199230
/gene="Usa1"
/locus_tag="P301_M10796"
/product="Usa1p"
CDS 196714..199230
/gene="Usa1"
/locus_tag="P301_M10796"
/note="Similar to YML029W 'Scaffold subunit of the Hrd1p
ubiquitin ligase that also promotes ligase
oligomerization; involved in ER-associated protein
degradation (ERAD); interacts with the U1 snRNP-specific
protein, Snp1p'"
/codon_start=1
/product="Usa1p"
/protein_id="EWG89284.1"
/translation="MSEYLAQTPCKFTIWSSEIDLIRTNLLVNAHPLSTVGRLLQYIH
YQIYKQLRAIYQPEEQCTNSEIPHTPLNSINTYFLSYEGRELSATCLLKDITSSSHPD
SNHFIRLQLEKRTSPSGSAFDLEYDMEGEFNSMNIQFEINTLSSQRIFNSMEPNLPIG
TTLARLEKLALERIKDFEKSAGNLCGIKEDHSVSDLQGFIIKGKQTPMFLNYGSDSDY
YKDLNLVDLIGIDFAPAHNSFFTFLFKMNHEQNSHIANDEERFVLEFISDATLSITQM
NVKPDTTVKQVKDFICSVYTHSLNLRRNDIKLIYKGQLLHENNFAGNSSKISEYIKEP
HEVKVHVQINQEYTESGPGFWNEVFNNPNIFQFMPPDTRSQSPVSFAPTQGRSPAAIR
GEERGIPYVTESGNDIVPTDELYRKCIINGDEVVFIPVSELNPQSSYLSVIKGDYGEI
KIPISSNDYRINGDNILLSPSAIEQLESALNFKIERPRDSTLLHPSGEHVRAADNTSS
ANDNNTVENDESAWNRRVVRPLRNSFPLLLVLIRTFYLIGYNSLVPFFIILEFGSFLP
WKYIILLSLLFIFRTVWNTQEVWNLWRDYLHLNEIDEVKFSQIKEFINSNSLTLNFYK
KCKDTQSAIDLLMIPNLHEQRLSVYSKYDIEYDTNTPDVGQLNLLFIKVLSGEIPKDA
LDELFKEFFELYETTRNMNTLYPQDSLNELLLMIWKESQKKDINTLPKYRRWFQTLCS
QIAEHNVLDVVLRYIIPDPVNDRVITAVIKNFVLFWVTLLPYVKEKLDDIVAQRARDR
EQPAPSAQQQENEDEALIIPDEEEPTATGAQPHLYIPDED"
gene 199490..200080
/gene="Tsa1"
/locus_tag="P301_M10806"
mRNA 199490..200080
/gene="Tsa1"
/locus_tag="P301_M10806"
/product="Tsa1p"
CDS 199490..200080
/gene="Tsa1"
/locus_tag="P301_M10806"
/note="Similar to YML028W 'Thioredoxin peroxidase, acts as
both a ribosome-associated and free cytoplasmic
antioxidant; self-associates to form a high-molecular
weight chaperone complex under oxidative stress; deletion
results in mutator phenotype'"
/codon_start=1
/product="Tsa1p"
/protein_id="EWG89285.1"
/translation="MVAQVQKQAPTFKKTAVVDGVFDEVSLDKYKGKYVVLAFIPLAF
TFVCPTEIIAFSEAAKKFEEQGAQVLFASTDSEYSLLAWTNIPRKEGGLGPINIPLLA
DTNHSLSRDYGVLIEEEGVALRGLFIIDPKGVIRHITINDLPVGRNVDEALRLVEAFQ
WTDKNGTVLPCNWTPGAATIKPTVEDSKEYFEAANK"
gene 200758..201915
/gene="Yox1"
/locus_tag="P301_M10816"
mRNA 200758..201915
/gene="Yox1"
/locus_tag="P301_M10816"
/product="Yox1p"
CDS 200758..201915
/gene="Yox1"
/locus_tag="P301_M10816"
/note="Similar to YML027W 'Homeodomain-containing
transcriptional repressor, binds to Mcm1p and to early
cell cycle boxes (ECBs) in the promoters of cell
cycle-regulated genes expressed in M/G1 phase; expression
is cell cycle-regulated; potential Cdc28p substrate'"
/codon_start=1
/product="Yox1p"
/protein_id="EWG89286.1"
/translation="MSQETKMLPSLSSLLSGTEISSSPVSPSFTNPRTSFHLDDRGTI
KLPPLNTSINRPRSVESALRHTVTSLHENSSAYGDDMLKHTQSDSALSSQLDSSQETV
DESHENLLLTPLNSKKRDYSVSSKKNDILTPLSAAKSIIIPSASKEKRRAFAFITHSQ
ETFPKKEPKIDNAPLARRKRRRTSSQELSILQAEFEKCPAPSKEKRIELAESCHMTEK
AVQIWFQNKRQAVKRQRIATSKSTTIIQTVSPPSPPLDVHATPLASRVKADILRDGSS
CSRSSSSSPLENTPPRPHHSLNRRSSTPSIKRSQALTFHLNPQKKTLTPVKTSPNSRV
NKLINSIDHSPSKAKRPVSNPSGSPKRKRKFGFKIVDQQPLKDLDPNAFRG"
assembly_gap 202083..202669
/estimated_length=587
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(203753..204712)
/gene="Yml6"
/locus_tag="P301_M10826"
mRNA complement(join(203753..204586,204686..204712))
/gene="Yml6"
/locus_tag="P301_M10826"
/product="Yml6p"
CDS complement(join(203753..204586,204686..204712))
/gene="Yml6"
/locus_tag="P301_M10826"
/note="Similar to YML025C 'Mitochondrial ribosomal protein
of the large subunit, has similarity to E. coli L4
ribosomal protein and human mitoribosomal MRP-L4 protein;
essential for viability, unlike most other mitoribosomal
proteins'"
/codon_start=1
/product="Yml6p"
/protein_id="EWG89287.1"
/translation="MTIKRNLVKTLQSIRYQATTATAHAESTLSPLPNAAIPPKYALV
TVRSFPSLEPLTFVPVPTSTVAAPLRRDILWRAVVYENDNRRVGASNPPGRSENGFSR
RKLMPQKGSGRARVGDANSPTRHNGGRALARTAPNDYTTELPSKVYSMAFNNALSHQY
KSGKLFVIGGEKVDLISPTPELDLNRLDLVNTNTVEGKEIFEGEVIFRKFLEEFQLKG
KRLLFITDKTREGLIKSSDPYKQKVDVIQKELVEVNDILRAQAVFIELEALEYLAMAH
QKEILHSVSN"
assembly_gap 205671..206036
/estimated_length=366
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(206338..208008)
/gene="Nse5"
/locus_tag="P301_M10836"
mRNA complement(206338..208008)
/gene="Nse5"
/locus_tag="P301_M10836"
/product="Nse5p"
CDS complement(206338..208008)
/gene="Nse5"
/locus_tag="P301_M10836"
/note="Similar to YML023C 'Essential subunit of the
Mms21-Smc5-Smc6 complex; required for cell viability and
DNA repair'"
/codon_start=1
/product="Nse5p"
/protein_id="EWG89288.1"
/translation="MDGALINSVLYVSPRNGAHYFVELTEKHLLAFEMLNSMCLLENY
DHVLLFLECQFGKSHNLAVIPFDIILVLFTLSTLSEYYKEPILRANDPYNTSRETLSR
RALKLLQKYLAILKEFDSEQYNLYDLELLRCQFFLAIDTLTPKKQKWSFDRFRRTKSE
KGVTYRQNASVDPELDQAKTFKNPYRSYISCLEQRNTILGNRLLNLKLNEPGEFINMI
LWTLSNSLQESTPLFLSSHEIWMPLLEILIDLFSCRQDYFIQHEVAQNVSKSLFVQRL
SESPLAVFFESLNTRNFANRFSEYVFLNCDYKLPSDNYATPVHPVYNGENTIVDTYIP
TIKCSPLYKSQKSLALRRKLIGSCFKLLLRVPDGHRLITPRIVADDVIQGISRTLASF
NDILQFKKFFMTENLSQESYFIPLLAEGTLSEILKDTQECVVILTLVENLSDGVSFCN
EVIGLVKSKCFAFTEQCSQTSYEEAVLNIEKCDVCLLVLLRYLLHLIGTEAILDAKEQ
LEMLHAIEKNDSGRRQWAKALNLGNNPPLLYPIVSQMFGVHDKSVIIE"
gene 208281..208844
/gene="Apt1"
/locus_tag="P301_M10846"
mRNA 208281..208844
/gene="Apt1"
/locus_tag="P301_M10846"
/product="Apt1p"
CDS 208281..208844
/gene="Apt1"
/locus_tag="P301_M10846"
/note="Similar to YML022W 'Adenine
phosphoribosyltransferase, catalyzes the formation of AMP
from adenine and 5-phosphoribosylpyrophosphate; involved
in the salvage pathway of purine nucleotide biosynthesis'"
/codon_start=1
/product="Apt1p"
/protein_id="EWG89289.1"
/translation="MSIASYAQELKLALHQYPNFPSEGILFEDFLPIFRNPGLFQKLI
DAFKLHLEEAFPEVKIDYIVGLESRGFLFGPTLALALGVGFVPVRKAGKLPGECFKAT
YEKEYGSDLFEIQKNAIPAGSNVIIVDDIIATGGSAAAAGGLVEQLEANLLEYNFVME
LDFLKGRSKLNAPVFTLLNAQKEALKK"
gene complement(209078..210157)
/gene="Ung1"
/locus_tag="P301_M10851"
mRNA complement(209078..210157)
/gene="Ung1"
/locus_tag="P301_M10851"
/product="Ung1p"
CDS complement(209078..210157)
/gene="Ung1"
/locus_tag="P301_M10851"
/note="Similar to YML021C Uracil-DNA glycosylase, required
for repair of uracil in DNA formed by spontaneous cytosine
deamination, not required for strand-specific mismatch
repair, cell-cycle regulated, expressed in late G1,
localizes to mitochondria and nucleus"
/codon_start=1
/product="Ung1p"
/protein_id="EWG89290.1"
/translation="MWCMRRLPTNSVMTVARKRKQTTIEDFFGTKKSTNEAPNKKGKS
GATFMTITNGAAIKTETKAVAKEANTDKYPANSNAKDVYSKNLSSNLRTLLSLELETI
DDSWFPHLMDEFKKPYFVKLKQFVTKEQADHTVFPPAKDIYSWTRLTPFNKVKVVIIG
QDPYHNFNQAHGLAFSVKPPTPAPPSLKNIYKELKQEYPDFVEDNKVGDLTHWASQGV
LLLNTSLTVRAHNANSHSKHGWETFTKRVVQLLIQDREADGKSLVFLLWGNNAIKLVE
SLLGSTSVGSGSKYPNIMVMKSVHPSPLSASRGFFGTNHFKMINDWLYNTRGEKMIDW
SVVPGTSLKEVQEANARLESESKDP"
gene 210493..212487
/locus_tag="P301_M10856"
mRNA 210493..212487
/locus_tag="P301_M10856"
/product="hypothetical protein"
CDS 210493..212487
/locus_tag="P301_M10856"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89291.1"
/translation="MVTTPLHSSPKSSLKSSTTSLRSVHTQSGRMGPSEPKHRTTKSW
SIWGGNDEEEPELSNKNKGKETAQDNSQENDNVSNEGTRLHDNQQTTILKETTRIEKG
EKDKKDRNAAIVEKNTRSRAWPFFWGRNKKDPEPTHNIPTDADNNTLSRLANSLNPAP
LTNTYIPYKPDAILIRDKGVKTAKKLTDDIGNQFPNIVVPSFDILPKQTIWNTVTSTI
WKWKTEYWDRRPSSRVREGEEQVQHSQNQLKEETNTVANEAKDEERLEHNRGSLFRVD
PWKKINLLSDYQSRPIRVLIVGVHGFFPTKIIRPFIGEPTGTSTKFVTEAEEIVKEYF
DQHKVPIEISKIALEREGEIFDRVDFFYEVMKHWSKEINNSDFIYFVSHSQGCPVTIM
LLAKLIKNGIINLDNSQFFNDDIQFCSSKKIISVLAMAGINNGPFYGADQTLFVRAYQ
TIEKDSLRELFEFQKFDSKQSQSFIEGLRTIISNNVKITFVGSINDQLVPLYSSICLF
ANHPNIFRAIFIDRGSQTPAFITRIVKIAGSLLNLGYNDHGIIKEISGSLAGTLTGGG
HSTIYNEKQVYHLGIKFALETTDLSEMYPIEYSPYKLSELGANPYRLPWCMRGLMYES
NKHFSNEEIKMLFKEFEEWEPETKQLKDIKNRLNGLKYRL"
gene 212808..213839
/gene="Ost6"
/locus_tag="P301_M10861"
mRNA 212808..213839
/gene="Ost6"
/locus_tag="P301_M10861"
/product="Ost6p"
CDS 212808..213839
/gene="Ost6"
/locus_tag="P301_M10861"
/note="Similar to YML019W 'Subunit of the
oligosaccharyltransferase complex of the ER lumen, which
catalyzes asparagine-linked glycosylation of newly
synthesized proteins; similar to and partially
functionally redundant with Ost3p'"
/codon_start=1
/product="Ost6p"
/protein_id="EWG89292.1"
/translation="MKWCSTYIIIWLAIIFHKFQKSTATASHNIDDILQLKGDTGVIT
VTADNYPLLSRGVPGYFNILYITMRGTNSNGMSCQLCHDFEKTYQAVADVIRSQAPQS
LNLFFTVDVNEVPQLVKDLKLQNVPHLVVYPPAESNKQSQFEWKTSPFYQYSLVPENA
ENTLQFGDFLAKILNISITVPQAFNVQEFVYYFVACMVVFIFIKKVILPKVTNKWKLF
SMILSLGILLPSITGYKFVEMNAIPFIARDAKNRIMYFSGGSGWQFGIEIFSVSLMYI
VMSALSVLLIYVPKISCVSEKMRGLLSSFLACVLFYFFSYFISCYLIKNPGYPIVFNF
EKMRFSVSL"
assembly_gap 213881..214079
/estimated_length=199
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(214081..215262)
/locus_tag="P301_M10871"
mRNA complement(214081..215262)
/locus_tag="P301_M10871"
/product="hypothetical protein"
CDS complement(214081..215262)
/locus_tag="P301_M10871"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89293.1"
/translation="MVSKDQTSFNKRWTLGLLMLGLVIILWVLSSFLINLIFEDDSYR
KPFFITYINTATFIFYLFPTAKAVVVNYKDTGRANVHRELIMEEEGTGSDSNRSVDMT
SPLLTNLEAGTHANQKKRLTLYETIKLSAEFCILWFTANLVTNASLAFTSVASQTILS
TTSSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQRHIADVSGDDN
DAVRVLIGNLLALAGAVLYGVYSTLLKREVGDETRVNMKIFFGFVGLFNLLFLWPSLI
VLDFFGWEPFSLPKDPKVVVIIFVNCLITFVSDFCWAKAMLLTSPLTVTVGLSITIPL
AMFGDVIFKHKTMSALYLFGATLILGSFFIINKSSEEEHFENSITASNYESVEVPAAN
N"
gene 215898..218033
/gene="Psp2"
/locus_tag="P301_M10876"
mRNA join(215898..215901,216262..218033)
/gene="Psp2"
/locus_tag="P301_M10876"
/product="Psp2p"
CDS join(215898..215901,216262..218033)
/gene="Psp2"
/locus_tag="P301_M10876"
/note="Similar to YML017W 'Asn rich cytoplasmic protein
that contains RGG motifs; high-copy suppressor of group II
intron-splicing defects of a mutation in MRS2 and of a
conditional mutation in POL1 (DNA polymerase alpha);
possible role in mitochondrial mRNA splicing'"
/codon_start=1
/product="Psp2p"
/protein_id="EWG89294.1"
/translation="MGTNNTSNNNGTTKKMSLEEFLGNDTLGESVWDEEDINLDAISN
TTNIDILKQTKAGEHQRDGHQQHPHGGHGPMNRSRFSNAGPFGGGSMGDFANHHHPLQ
HQQGPPYIVKFSDLPPRFSNFDIEDLFQAKFTKFIKFKLFWEINKNPSISTLKSGSIF
DQNFKRDSKVAFVELYTSRDMDKILNYWTTPLKEIYHITTAPAEFEDFKDYSTKVKLL
TDPKDDAGKPFITKTQRSKSNPFGSAKPVDTQSKILDIEEKMENLHVEDTTTLRASLI
PSSDSMATTATGSKITILKKQTPTEEESHSATPTPKPLSYSEVVERSVVNETSKKGTP
LSKLGSPALELQSKPDKSDEFKGGDEQGFEKGGDDKAQLDVSNDKDKGSETDVDKQFT
FKNVEREHSMSRTKYNGNHNNNNGNFRGSNRYRGGPNGSSYKGGHNNRGNRGGYRSGS
SYNNNTNDNNNNNNNNSSSNNNNGSRYHDRQNNEEGLTSDSSLDASGNKKNDFTNSTS
NTQQYSIFKPASGFLGQGNNDSIRNNGRGNYNSSGMNGGSRGRGFGRGRGFGRGAYNN
RGSRGGRGSSGNYSNYNNRTTDMPL"
assembly_gap 218260..218672
/estimated_length=413
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(218769..220847)
/gene="Ppz1"
/locus_tag="P301_M10891"
mRNA complement(218769..220847)
/gene="Ppz1"
/locus_tag="P301_M10891"
/product="Ppz1p"
CDS complement(218769..220847)
/gene="Ppz1"
/locus_tag="P301_M10891"
/note="Similar to YML016C 'Serine/threonine protein
phosphatase Z, isoform of Ppz2p; involved in regulation of
potassium transport, which affects osmotic stability, cell
cycle progression, and halotolerance'"
/codon_start=1
/product="Ppz1p"
/protein_id="EWG89295.1"
/translation="MGNSSSKSSKKDSHSNSSSRNPRPQVSRTETSHSVKSAKSNKSS
RSRRSLPSSSTTNTNSNVPDPSTPSKPNLEVNHQRHSSHTNRYHFPSSSHSHSNSQNE
LLTTPSSSSTKRPSTSRRSSYNTKAAADLPPSMIQMEPKSPILKTNNSSTHVSKHKSS
YSSTYYENALTDDDNDDKDNDISHTKRFSRSSNSRPSSIRSGSVSRRKSDVTHEEPNN
GSYSSNNQENYLVQALTRSNSHASSLHSRKSSFGSDGNTAYSTPLNSPGLSKLTDHSG
EYFTSNSTSSLNHHSSRDIYPSKHISNDDDIENSSQLSDIHASMENVNDKNNNITDSK
KDPNEEFNDIMQSSGNKNAPKKFKKPIDIDETIQKLLDAGYAAKRTKNVCLKNNEILQ
ICIKAREIFLSQPSLLELSPPVKIVGDVHGQYGDLLRLFTKCGFPPSSNYLFLGDYVD
RGKQSLETILLLFCYKIKYPENFFLLRGNHECANVTRVYGFYDECKRRCNIKIWKTFI
DTFNTLPLAAIVAGKIFCVHGGLSPVLNSMDEIRHVVRPTDVPDFGLINDLLWSDPTD
SPNEWEDNERGVSYCYNKVAINKFLNKFGFDLVCRAHMVVEDGYEFFNDRSLVTVFSA
PNYCGEFDNWGAVMSVSEGLLCSFELLDPLDSAALKQVMKKGRQERKLANQQQQMMET
SITNDNESQQ"
assembly_gap 221040..221178
/estimated_length=139
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(221300..222340)
/gene="Taf11"
/locus_tag="P301_M10911"
mRNA complement(221300..222340)
/gene="Taf11"
/locus_tag="P301_M10911"
/product="Taf11p"
CDS complement(221300..222340)
/gene="Taf11"
/locus_tag="P301_M10911"
/note="Similar to YML015C TFIID subunit (40 kDa), involved
in RNA polymerase II transcription initiation, similar to
histone H3 with atypical histone fold motif of Spt3-like
transcription factors"
/codon_start=1
/product="Taf11p"
/protein_id="EWG89296.1"
/translation="MTEPQGPLDTIPKVNYPPILTIANYFSTKQMIDQVISEDQDYVT
WKLQNLRTGGTSINNQLNKYPKYKYQKTRINQQDLDSINKVPENLIFPQDILQQQTQN
SNYEDTNTNEDENEKLAQDEQFKLLVTNLDKDQTNRFEVFHRTSLNKTQVKKLASTVA
NQTISENIRVFLQAVGKIYAGEIIELAMIVKNKWLTSQMCIEFDKRTKIGYKLKKYLK
KLTFSIIENQQYKQDYQSDSVPEDEPDFYFDDEEVDKRESTLGNSLLQSKSLQQSDHN
SQDLKLQLIEQYNKLVVQFNKLDVSIEKYNNSPLLPEHIREAWRLYRLQSDTLPNAYW
RTQGEGQGSMFR"
gene 222536..223375
/gene="Trm9"
/locus_tag="P301_M10916"
mRNA 222536..223375
/gene="Trm9"
/locus_tag="P301_M10916"
/product="Trm9p"
CDS 222536..223375
/gene="Trm9"
/locus_tag="P301_M10916"
/note="Similar to YML014W 'tRNA methyltransferase,
catalyzes esterification of modified uridine nucleotides
in tRNA(Arg3) and tRNA(Glu), likely as part of a complex
with Trm112p; deletion confers resistance to zymocin'"
/codon_start=1
/product="Trm9p"
/protein_id="EWG89297.1"
/translation="MEINQAAEKEQEYVHKVYNEIAPHFSQTRYKPWPIVTQFLKTRP
MGSIGIDVGCGNGKYLGVNPDIYIIGSDRSDGLIECARGINPSYNLLVADGLNLPHKN
ETFDFAISIAVVHHWSTRERRVEVIRHVLSKLRQGGQALIYCWALEQGSSRRGYHEGM
EQDVFVPWVLPKSKSKPKTKSTPPSKVKTRPKPNLMNIPPKERSEYLQRWKEEQQRSK
SLDDNDEKQQQDQEQEREEVKYRYYHLYREGELAEDCRQAGAAVHSEGFERDNWWVVA
QKR"
gene 223460..225214
/gene="Ubx2"
/locus_tag="P301_M10921"
mRNA 223460..225214
/gene="Ubx2"
/locus_tag="P301_M10921"
/product="Ubx2p"
CDS 223460..225214
/gene="Ubx2"
/locus_tag="P301_M10921"
/note="Similar to YML013W 'Protein involved in
ER-associated protein degradation; proposed to coordinate
the assembly of proteins involved in ERAD; contains a UBX
(ubiquitin regulatory X) domain and a ubiquitin-associated
(UBA) domain'"
/codon_start=1
/product="Ubx2p"
/protein_id="EWG89298.1"
/translation="MPVVNHEDSEFHLSHTEEDKLNEFQVITNFPPGDLPDVVRLLRN
HGWQLEPALSRYFDGEWKGEPDQMGESTQTSTPMAETLVPPALGPRPLLFTASLPVVR
PLPANFRNDFRTIGLNGRSNTVWSMFESFSYDGNPFLFILLLIPRIINRLSATIFTFF
CTLLSLHSISGGGNSGKPKISKVPKAPTRETHIPLAEILGDTKDKDAFCELKSFKPDI
SFNEALRIAKEEFKFMLLILVGDTYDTDTDTVDVNSKLLLEKILLNKKTLQYLRKIDN
DLIIYLKCVHELEPWLVARQLGVRNTPEIFLIANVANKASHSETLPSQRLSILGKLKV
NSLNRFLQSLTNVVEKYTPELVVNKTEMHELRMSREIKKLQEDAYKKSLEMDRIKAIE
KEKSLKHAQDLKLNSTARQLKWLKACIDEIQPFETTGKQATLQFRTSSGKRFIKKFPS
MTTLYQIYQSIGCHIYLAVYSSDPVEWSNALQDKIRQLSADDDVLCFKEGQLETATAT
TIEELGHIINNELTSFDLERGKLEFDFELVSPFPKYTVHPNEHMSVDQVPQLWPNGSL
LVEALDEEDEEDEENEEQ"
gene complement(224848..225225)
/locus_tag="P301_M10926"
mRNA complement(224848..225225)
/locus_tag="P301_M10926"
/product="hypothetical protein"
CDS complement(224848..225225)
/locus_tag="P301_M10926"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89299.1"
/translation="MLFYHCSSFSSSSSSSSSSASTRRLPFGHSWGTWSTDMCSLGCT
VYLGKGDTNSKSNSSLPRSRSNEVSSLLMMWPSSSIVVAVAVSSCPSLKQSTSSSADN
CLILSWRALDHSTGSLEYTARYM"
gene 225428..226063
/gene="Erv25"
/locus_tag="P301_M10931"
mRNA 225428..226063
/gene="Erv25"
/locus_tag="P301_M10931"
/product="Erv25p"
CDS 225428..226063
/gene="Erv25"
/locus_tag="P301_M10931"
/note="Similar to YML012W Protein that forms a
heterotrimeric complex with Erp1, Erp2p, and Emp24, member
of the p24 family involved in endoplasmic reticulum to
Golgi transport"
/codon_start=1
/product="Erv25p"
/protein_id="EWG89300.1"
/translation="MQVLQLWLTTLISLVVAVQGLHFDIAASTDPEQVCIRDFVTEGQ
LVVVDIHSDGSVGDGQKLNLFVRDSVGNEYRRKRDFAGDVRVAFTAPSSTAFDVCFEN
QAQYRGRSLSRAIELDIESGAEARDWNKISANEKLKPIEVELRRVEEITDEIVDELTY
LKNREERLRDTNESTNRRVRNFSILVIIVLSSLGVWQVNYLKNYFKTKHII"
gene complement(226207..226740)
/gene="Rad33"
/locus_tag="P301_M10936"
mRNA complement(226207..226740)
/gene="Rad33"
/locus_tag="P301_M10936"
/product="Rad33p"
CDS complement(226207..226740)
/gene="Rad33"
/locus_tag="P301_M10936"
/note="Similar to YML011C 'Protein involved in nucleotide
excision repair; green fluorescent protein (GFP)-fusion
protein localizes to the nucleus'"
/codon_start=1
/product="Rad33p"
/protein_id="EWG89301.1"
/translation="MSKSTNVCYERVELFENPKVPIEVEDEILEKYAESSLDHDMTVN
ELPRFFKDLQLEPTIWKLVRNEDVIIEGTDVIDFTKLVRCTCQLLILMNNLTVIDDLW
SMLIRNCGRDVDFPQVALRDHVLSVKDLQKISNLIGADQSSGTIEMISCATDGKRLFM
TYLDFGCVLGKLGYLKM"
gene 226986..230175
/gene="Spt5"
/locus_tag="P301_M10941"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 229742..230218
/locus_tag="P301_M10946"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(229982..230309)
/locus_tag="P301_M10951"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(230612..230824)
/gene="Mrpl39"
/locus_tag="P301_M10956"
mRNA complement(230612..230824)
/gene="Mrpl39"
/locus_tag="P301_M10956"
/product="Mrpl39p"
CDS complement(230612..230824)
/gene="Mrpl39"
/locus_tag="P301_M10956"
/note="Similar to YML009C Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl39p"
/protein_id="EWG89302.1"
/translation="MVKVKSKNSVIKLLSTAASGYSRYISIKKGAPLVTQVRYDPVVK
RHVLFKEAKKRKVAERKPLDFLRTAK"
gene complement(231155..232306)
/gene="Erg6"
/locus_tag="P301_M10961"
mRNA complement(231155..232306)
/gene="Erg6"
/locus_tag="P301_M10961"
/product="Erg6p"
CDS complement(231155..232306)
/gene="Erg6"
/locus_tag="P301_M10961"
/note="Similar to YML008C 'Delta(24)-sterol
C-methyltransferase, converts zymosterol to fecosterol in
the ergosterol biosynthetic pathway by methylating
position C-24; localized to both lipid particles and
mitochondrial outer membrane'"
/codon_start=1
/product="Erg6p"
/protein_id="EWG89303.1"
/translation="MSETELRKRQAQFTRELHGDDIGKKTGLSALMSKNNSAQKEAVQ
KYLRNWDGRTDKDAEERRLEDYNEATHSYYNVVTDFYEYGWGSSFHFSRFYKGESFAA
SIARHEHYLAYKAGIQRGDLVLDVGCGVGGPAREIARFTGCNVIGLNNNDYQIAKAKY
YAKKYNLSDQMDFVKGDFMKMDFEENTFDRVYAIEATCHAPKLEGVYSEIYKVLKPGG
TFAVYEWVMTDKYDENNPEHRKIAYEIELGDGIPKMFHVDVARKALKNCGFEVLVSED
LADNDDEIPWYYPLTGEWKYVQNLANLATFFRTSYLGRQFTTAMVTVMEKLGLAPEGS
KEVTAALENAAVGLVAGGKSKLFTPMMLFVARKPENAETPSQTSQEATQ"
gene complement(232478..232588)
/locus_tag="P301_M10971"
mRNA complement(232478..232588)
/locus_tag="P301_M10971"
/product="hypothetical protein"
CDS complement(232478..232588)
/locus_tag="P301_M10971"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89304.1"
/translation="MVHFIFIALRSMRFMRRLVRNLQYLLLPITSSLLFI"
gene 233163..235133
/gene="Yap1"
/locus_tag="P301_M10981"
mRNA 233163..235133
/gene="Yap1"
/locus_tag="P301_M10981"
/product="Yap1p"
CDS 233163..235133
/gene="Yap1"
/locus_tag="P301_M10981"
/note="Similar to YML007W 'Basic leucine zipper (bZIP)
transcription factor required for oxidative stress
tolerance; activated by H2O2 through the multistep
formation of disulfide bonds and transit from the
cytoplasm to the nucleus; mediates resistance to cadmium'"
/codon_start=1
/product="Yap1p"
/protein_id="EWG89305.1"
/translation="MSVSTAKRSLDVVSPGSLAEFEGSKSRHDEIENEHRRTGTRDGE
DSEQPKKKGSKTSKKQDLDPETKQKRTAQNRAAQRAFRERKERKMKELEKKVQSLESI
QQQNEVEATFLRDQLITLVNELKKYRPETRNDSKVLEYLARRDPNLLFSKNNVNHSNS
EPIDTPNDDIQENVKQKMNFTFQYPLDNDNDNDDSKNVGKQLPSPNDPSHSAPMPINQ
TQKKLSDATDSSSATLDSLSNSNDVLNNTPNSSTSMDWLDNVIYTNRFVSGDDGSNSK
TKNLDSNMFSNDFNFENQFDEQVSEFCSKMNQVCGTRQCPIPKKPISALDKEVFASSS
ILSSNSPALTNTWESHSNITDNTPANVIATDATKYENSFSGFGRLGFDMSANHYVVND
NSTGSTDSTDSTGSTGSTGNKNKKNNNNSDDVLPFISESPFDMNQVTNFFSPGSTGIG
NNAASNTNPSLLQSSKEDIPFINANLAFPDDNSTNIQLQPFSESQSQNKFDYDMFFRD
SSKEGNNLFGEFLEDDDDDKKAANMSDDESSLIKNQLINEEPELPKQYLQSVPGNESE
ISQKNGSSLQNADKINNGNDNDNDNDVVPSKEGSLLRCSEIWDRITTHPKYSDIDVDG
LCSELMAKAKCSERGVVINAEDVQLALNKHMN"
gene complement(235429..237801)
/gene="Gis4"
/locus_tag="P301_M10986"
mRNA complement(235429..237801)
/gene="Gis4"
/locus_tag="P301_M10986"
/product="Gis4p"
CDS complement(235429..237801)
/gene="Gis4"
/locus_tag="P301_M10986"
/note="Similar to YML006C CAAX box containing protein of
unknown function, proposed to be involved in the RAS/cAMP
signaling pathway"
/codon_start=1
/product="Gis4p"
/protein_id="EWG89306.1"
/translation="MQKSVRVGDYFDNDDNGLWSWYLTNLRLGDFEELIGNQLKYTLL
KRFLNSHFYGDNNISARPNKKILLVSIPENVHEDISILEIFLKDYFHLEKLEHIQISK
LTHSHCYNHENHYLLTDNLNNFQDPTFLEFPSTSWQVQKNSKALNNNNRNSIPLPTIS
SSKASNGKLESNVSDDQWSNINTQTSTATRTNTNTRTLTSPDTVDINVTSVNSQSNNN
DTSQDNENEVDEEDATSSIVLNFSHSRTVDSKPNRLPKIFPSYTNEDYTPSHSEIMSI
DSFAGEDVSSTYPGQDLSLTTARREDESGQDEVEDHYSRVSHDLGDESIDQASYSMES
SVSYTSYSSSSNSSSAHYSLSSSSRGNPKRENIDHTNATYVSELSSITSSIDNLTTST
TPEEEDNLIHHNYDAQGYGSGEDDGEEVYDDEDLSSSDYSVLSILPSISICDSLGYFR
LVLQSILIQDPDTKEIFTAIRQSNNKPTMASVTDDWLLYDSNFSMNNLQILTLQDLLD
IKRSFPKILFYTMVIVTNSGKQVEEELKNPNYDNREGISKEQPLDSELSLTNDPQQYF
PTAYNNGYNDYIDDEDDEDDGDDASLSEQSGPQMYIPTRMESNVTTAHRSIRTVNSIG
EWAFNRHNSVTKIDKSNSNELDNSKTGESTVLSSEPHPMTQLSNSNTTSSNFSHSLKT
KNSHKPNSKGNNESNSKNELKKIKSSINAMSAVERSKSLPLPTLLKSLSGIDNPTHAT
NKDRKRWXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLCHS"
assembly_gap 235444..235558
/estimated_length=115
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(238541..238622)
/locus_tag="P301_M10991"
tRNA complement(238541..238622)
/locus_tag="P301_M10991"
/product="tRNA-Ser"
/note="TS(AGA)M"
gene 239605..240993
/gene="Trm12"
/locus_tag="P301_M11001"
mRNA 239605..240993
/gene="Trm12"
/locus_tag="P301_M11001"
/product="Trm12p"
CDS 239605..240993
/gene="Trm12"
/locus_tag="P301_M11001"
/note="Similar to YML005W 'S-adenosylmethionine-dependent
methyltransferase of the seven beta-strand family;
required for wybutosine formation in
phenylalanine-accepting tRNA'"
/codon_start=1
/product="Trm12p"
/protein_id="EWG89307.1"
/translation="MSDETMPVEFLVSDKRLLKTIKVKLETNGLFVTPIYSDNDNKVI
KSSIEDLNHPLAVEINNIAGVKARFHESSNLERSEGRLKHQSNSITEFTKSFLKDHGL
ANDKIFLSHLLDHLPLKYTIYPPVVLFNNSTVRSFNHPIWQKAFQLKLFDPNEYYREL
LCFLSPGKPSKGTSLHPNNRLLTHLAINNPITEADVLRRPFNIQPLYGKLIDDSILDD
NDNTLWENPSQEQLNSSIWCKVIQNGVTQIWSPVFTMFSRGNIKEKKRVLTTFPDICN
NDVVDLYAGIGYFTFSYLTKGARTLFAFELNPWSVEGLKRGLKANGFSKSGNCHVFQE
SNEMCVQRLTEFLSQNPGFRLRIRHINLGLLPSSKQGWPLAIKLIYLQGASLEKVTMH
IHENVHIDAIEDGSFEKNVIVELDAINESIALIRNRGIKLQFVRSKVERIKTFAPDIW
HVCVDVDVIVST"
gene complement(241088..242068)
/gene="Glo1"
/locus_tag="P301_M11006"
mRNA complement(241088..242068)
/gene="Glo1"
/locus_tag="P301_M11006"
/product="Glo1p"
CDS complement(241088..242068)
/gene="Glo1"
/locus_tag="P301_M11006"
/note="Similar to YML004C 'Monomeric glyoxalase I,
catalyzes the detoxification of methylglyoxal (a
by-product of glycolysis) via condensation with
glutathione to produce S-D-lactoylglutathione; expression
regulated by methylglyoxal levels and osmotic stress'"
/codon_start=1
/product="Glo1p"
/protein_id="EWG89308.1"
/translation="MSTDSTRYPIQIEKASNDPTLLLNHTCLRVKDPARAVKFYTEHF
GMKLLSRKDFEEAKFSLYFLSFPKDDIPKNKNGEPDVFSAHGVLELTHNWGTEKNPDY
KINNGNEEPHRGFGHICFSVSDINKTCEELESQGVKFKKRLSEGRQKDIAFALDPDGY
WIELITYSREGQEYPKGSVGNKFNHTMIRIKNPTRSLEFYQNVLGMKLLRTSEHESAK
FTLYFLGYGVPKTDSVFSCESVLELTHNWGTENDPNFHYHNGNSEPQGYGHICISCDD
AGALCKEIEVKYGDKIQWSPKFNQGRMKNIAFLKDPDGYSIEVVPYGLIA"
gene 242869..246142
/locus_tag="P301_M11016"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 242870..243742
/locus_tag="P301_M11011"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 244075..244171
/estimated_length=97
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 246561..>247145
/gene="Ypt7"
/locus_tag="P301_M11026"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 247146..247571
/estimated_length=426
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(248407..250524)
/gene="Cdc5"
/locus_tag="P301_M11031"
mRNA complement(248407..250524)
/gene="Cdc5"
/locus_tag="P301_M11031"
/product="Cdc5p"
CDS complement(248407..250524)
/gene="Cdc5"
/locus_tag="P301_M11031"
/note="Similar to YMR001C 'Polo-like kinase with multiple
functions in mitosis and cytokinesis through substrate
phosphorylation, also functions in adaptation to DNA
damage during meiosis; has similarity to Xenopus Plx1 and
S. pombe Plo1p; possible Cdc28p substrate'"
/codon_start=1
/product="Cdc5p"
/protein_id="EWG89309.1"
/translation="MSLGPLKAINDKQLNTRSKLVHTPVKGNTADLVGKENHFKQTKR
LDPNNDHHHQPAQKKKREKLSALCKTPPSLIKTRGKDYHRGHFLGEGGFARCFQIKDD
SGEIFAAKTVAKASIKSEKTRKKLLSEIQIHKSMSHPNIVQFIDCFEDDSNVYILLEI
CPNGSLMELLKRRKVLTEPEVRFFTTQICGAIKYMHSRRVIHRDLKLGNIFFDSNYNL
KIGDFGLAAVLANESERKYTICGTPNYIAPEVLMGKHSGHSFEVDIWSLGVMLYALLI
GKPPFQARDVNTIYERIKCRDFSFPRDKPISDEGKILIRDILSLDPIERPSLTEIMDY
VWFRGTFPPSIPSTVMSEAPNFEDIPEEQSLVNFKDCMEKSLLLESMSSDKIQRQKRD
YISSIKSSIDKLEEYHQNRPFLPHSLSPGGTKQKYKEVVDIEAQRRLNDLAREARIRR
AQQAVLRKELIATSTNVIKSEISLRILASECHLTLNGIVEAEAQYKMGGLPKSRLPKI
KHPMIVTKWVDYSNKHGFSYQLSTEDIGVLFNNGTTVLRLADAEEFWYISYDDREGWV
ASHYLLSEKPRELSRHLEVVDFFAKYMKANLSRVSTFGREEYHKDDVFLRRYTRYKPF
VMFELSDGTFQFNFKDHHKMAISDGGKLVTYISPSHESTTYPLVEVLKYGEIPGYPES
NFREKLTLIKEGLKQKSTIVTVD"
gene complement(250735..250965)
/locus_tag="P301_M11036"
mRNA complement(250735..250965)
/locus_tag="P301_M11036"
/product="hypothetical protein"
CDS complement(250735..250965)
/locus_tag="P301_M11036"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89310.1"
/translation="MIFECTSFETRHILNSYSCATQLSYNIYRITEKVNPIQAAVSKN
NKPFVSNSDSAFFPIFSSFNFVAYTTLTFKKT"
gene 251581..252057
/gene="Mic17"
/locus_tag="P301_M11041"
mRNA 251581..252057
/gene="Mic17"
/locus_tag="P301_M11041"
/product="Mic17p"
CDS 251581..252057
/gene="Mic17"
/locus_tag="P301_M11041"
/note="Similar to YMR002W 'Mitochondrial intermembrane
space protein, required for normal oxygen consumption;
contains twin cysteine-x9-cysteine motifs'"
/codon_start=1
/product="Mic17p"
/protein_id="EWG89311.1"
/translation="MARSRGSSRPISRSRPTQTRSASTMAAPVHPQQQQQQQPNAYSH
PPAAGAQTRQPGMFAQMASTAAGVAVGSTIGHTLGAGITGMFSGSGSDSAPVEQQQQN
IANTSGQTQTDQQLGRTCEIDARNFTRCLDENNGNFQICDYYLQQLKACQEAARQY"
gene 252512..253108
/gene="Aim34"
/locus_tag="P301_M11046"
mRNA 252512..253108
/gene="Aim34"
/locus_tag="P301_M11046"
/product="Aim34p"
CDS 252512..253108
/gene="Aim34"
/locus_tag="P301_M11046"
/note="Similar to YMR003W 'Protein of unknown function;
GFP-fusion protein localizes to the mitochondria; null
mutant is viable and displays reduced frequency of
mitochondrial genome loss'"
/codon_start=1
/product="Aim34p"
/protein_id="EWG89312.1"
/translation="MSISLLGRIVSQQFSGIRAAGPGRSLYLPFTLLLKQPGAYKVNL
HRYVHSTQTKSHLSFLMNNNDITPFQKFTVKVLKEQCKSRGLKLSGRKSDLLQRLITH
DSCSNKKSSVKINEPKKKRILINDPIKITKKLVSDKTFRTIEKNISSLQNTPVIETPC
DVHSHLQPRDRIFLLGFFMLSCLWWNLEPQESKPTIDH"
gene 253410..254945
/gene="Mvp1"
/locus_tag="P301_M11051"
mRNA 253410..254945
/gene="Mvp1"
/locus_tag="P301_M11051"
/product="Mvp1p"
CDS 253410..254945
/gene="Mvp1"
/locus_tag="P301_M11051"
/note="Similar to YMR004W 'Protein required for sorting
proteins to the vacuole; overproduction of Mvp1p
suppresses several dominant VPS1 mutations; Mvp1p and
Vps1p act in concert to promote membrane traffic to the
vacuole'"
/codon_start=1
/product="Mvp1p"
/protein_id="EWG89313.1"
/translation="MDNYEGSDPWNTSSNAWTKDDDHVVSTTNNEPSLNGISGEFNAL
NFSTPLDTNEEDTGFLPTNDVLEESIWDDSRNPLGATGMSQTPNIAANETVIDKNDAR
DQNIEESEADLLDWTNNVRKTYRPLDADIIIIEEIPEREGLLFKHANYLVKHLIALPS
TSPSEERTVVRRYSDFLWLREILLKRYPFRMIPELPPKRIGSQNADQLFLKKRRIGLS
RFINLVMKHPKLSNDDLVLTFLTVRTDLTSWRKQATYDTSNEFADKKISQEFMKMWKK
EFAEQWNQAASCIDTSMELWYRITLLLERHEKRIMQMVHERNFXXXXXXXXXEVTPKL
YPVQQNDTILDINNNLSIIKKHLETTSSLCKQETEEISGTLSPKFKIFTDILLSLRSL
FERYKIMAANNVVELQRHVELNKEKLESMKGKPDVSGAEYDRIKKIIQKDRRSIIEQS
NRAWLIRQCILEEFTIFQETQFLITRAFQDWAKLNSNHAGLKLNEWEKLVTSIMDMPI
SRE"
assembly_gap 254369..254393
/estimated_length=25
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 255078..255128
/estimated_length=51
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 255443..256608
/gene="Taf4"
/locus_tag="P301_M11061"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(256957..259077)
/gene="Plb2"
/locus_tag="P301_M11066"
mRNA complement(256957..259077)
/gene="Plb2"
/locus_tag="P301_M11066"
/product="Plb2p"
CDS complement(256957..259077)
/gene="Plb2"
/locus_tag="P301_M11066"
/note="Similar to YMR006C 'Phospholipase B
(lysophospholipase) involved in phospholipid metabolism;
displays transacylase activity in vitro; overproduction
confers resistance to lysophosphatidylcholine'"
/codon_start=1
/product="Plb2p"
/protein_id="EWG89314.1"
/translation="MQLRNILQASSLISGLSLAADSSSTTGDGYAPSIIPCPSDDTSL
VRNASGLSTAETDWLKKRDAYTKEALHSFLSRATSNFSDTSLLSTLFSSNSSNVPKIG
IACSGGGYRAMLGGAGMIAAMDNRTDGANEHGLGGLLQSSTYLSGLSGGNWLTGTLAW
NNWTSVQEIVDHMSESDSIWNITKSIVNPGGSNLTYTIERWESIVQEVQAKSDAGFNI
SLSDLWARALSYNFFPSLPDAGSALTWSSLRDVDVFKNGEMPLPITVADGRYPGTTVI
NLNATLFEFTPFEMGSWDPSLNAFTDVKYLGTNVTNGKPVNKDQCVSGYDNAGFVIAT
SASLFNEFSLEASTSTYYKMINSFANKYVNNLSQDDDDIAIYAPNPFKDTEFVDRNYT
SSIVDADDLFLVDGGEDGQNLPLVPLIKKERDLDVVFALDISDDTDESWPSGVCMTNT
YERQYSKQGKGMAFPYVPDVNTFLNLGLTNKPTFFGCDAKNLTDLEYIPPLVVYIPNT
KHSFNGNQSTLKMNYNVTERLGMIRNGFEAATMGNFTDDSNFLGCIGCAIIRRKQESL
NATLPPECTKCFADYCWNGTLSTSANPELSGNSTYQSGAIASAISEATDGIPITALLG
SSTSGNTTSNSTTSTSSNVTSNSNSSSNTTLNSNSSSSSISSSTARSSSSTANKANAA
AISYANTNTLMSLLGAITALFGLI"
gene complement(259986..261962)
/gene="Plb1"
/locus_tag="P301_M11086"
mRNA complement(259986..261962)
/gene="Plb1"
/locus_tag="P301_M11086"
/product="Plb1p"
CDS complement(259986..261962)
/gene="Plb1"
/locus_tag="P301_M11086"
/note="Similar to YMR008C Phospholipase B
(lysophospholipase) involved in lipid metabolism, required
for deacylation of phosphatidylcholine and
phosphatidylethanolamine but not phosphatidylinositol"
/codon_start=1
/product="Plb1p"
/protein_id="EWG89315.1"
/translation="MKLQSLLVSAAVLTSLTENVNAWSPNNSYVPANVTCDDDINLVR
EASGLSDNETEWLKKRDAYTKEALHSFLNRATSNFSDTSLLSTLFGSNSSNMPKIAVA
CSGGGYRAMLSGAGMLAAMDNRTDGANEHGLGGLLQGATYLAGLSGGNWLTSTLAWNN
WTSVQAIVDNTTESNSIWDISHSILTPGGINIFKTGSRWDDISDDVQDKKDAGFNISL
ADVWGRALAYNFWPSLHRGGVGYTWSTLREADVFKNGEMPFPITVADGRYPGTTVINL
NATLFEFNPFEMGSWDPTLNAFTDVKYLGTNVTNGKPVNKGQCIAGFDNTGFITATSS
TLFNQFLLRLNSTDLPSFIANLATDFLEDLSDNSDDIAIYAPNPFKEANFLQKNATSS
IIESEYLFLVDGGEDNQNIPLVPLLQKERELDVIFALDNSADTDDYWPDGASLVNTYQ
RQFGSQGLNLSFPYVPDVNTFVNLGLNKKPTFFGCDARNLTDLEYIPPLIVYIPNSRH
SFNGNQSTFKMSYSDSERLGMIKNGFEAATMGNFTDDSDFLGCVGCAIIRRKQQNLNA
TLPSECSQCFTNYCWNGTIDSRSVSGVGNDDYSSSASLSASAAAASASASASASGSST
HKKNAGNALVNYSNLNTNTFIGVLSVISAVFGLI"
gene 263479..264018
/gene="Adi1"
/locus_tag="P301_M11096"
mRNA 263479..264018
/gene="Adi1"
/locus_tag="P301_M11096"
/product="Adi1p"
CDS 263479..264018
/gene="Adi1"
/locus_tag="P301_M11096"
/note="Similar to YMR009W 'Acireductone dioxygenease
involved in the methionine salvage pathway; ortholog of
human MTCBP-1; transcribed with YMR010W and regulated
post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1
mRNA is induced in heat shock conditions'"
/codon_start=1
/product="Adi1p"
/protein_id="EWG89316.1"
/translation="MVKVYIHDNKVDSDYRAPHNSGTELSLDELAKLGVIYKYCANEE
EVNEIARQREYKNRDVVNICEGSFKSEAEFNEKLATFYQEHLHEDEEIRYCLEGAGYF
DVRDASTPENWIRCLVESGDLLILPPGIYHRFTLTTSNHIKALRLFKDEPKWQAINRS
NQADSLPVRKDYIALINQY"
gene 264476..265693
/locus_tag="P301_M11101"
mRNA 264476..265693
/locus_tag="P301_M11101"
/product="hypothetical protein"
CDS 264476..265693
/locus_tag="P301_M11101"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89317.1"
/translation="MSTTGPLDATLIRDVAVATATKASYDMSDTLYSYLPKVDQFYIP
EWLTMQFIANNLISFTPLFSYGTTIISIEKCKTALGFSIDICATMLIASILRISYYLI
TPYEITLLRQSLVMIFIQLILLRTSLKYRPDEYKYQNLTDVESLSHLIHDIWFEFFSC
INRPKFLSEDWKNLIKSLSFTNLLKFSFKIFLAFFYKILKFFDPNFKRIGAFWQWDDD
KNFWRFLALFATVQILVTFFISNILNWDSLAQGLGSIIGSLGLLVESLLPLPQIAILY
KLKSVQGFKLILLVSWLCGDTLKITYLIFGAKNISALFVIFALFQMSLDFYIGGQYIY
YRYYYPKLRHQHHPNDSNSPSDEDESEMYELDLFNTLQKDVEKALKQDSNDTSDSPQD
DQVGKSQAQAVTV"
gene 267455..269080
/gene="Hxt2"
/locus_tag="P301_M11116"
mRNA 267455..269080
/gene="Hxt2"
/locus_tag="P301_M11116"
/product="Hxt2p"
CDS 267455..269080
/gene="Hxt2"
/locus_tag="P301_M11116"
/note="Similar to YMR011W High-affinity glucose
transporter of the major facilitator superfamily,
expression is induced by low levels of glucose and
repressed by high levels of glucose"
/codon_start=1
/product="Hxt2p"
/protein_id="EWG89318.1"
/translation="MSEFATSRVESGSQQTSIHSTPIVQKLETDESPIQTKSEYTNAE
LPAKPIAAYWTVICLCLMIAFGGFVFGWDTGTISGFVNQTDFKRRFGQMKSDGTYYLS
DVRTGLIVGIFNIGCAIGGLTLGRLGDMYGRRIGLMCVVLVYIVGIVIQIASSDKWYQ
YFIGRIISGMGVGGIAVLSPTLISETAPKHIRGTCVSFYQLMITLGIFLGYCTNYGTK
DYSNSVQWRVPLGLNFAFAIFMIAGMLMVPESPRFLVEKGRYEDAKRSLAKSNKVTIE
DPSIVAEMDTIMANVETERLAGNASWGELFSNKGAILPRVIMGIMIQSLQQLTGNNYF
FYYGTTIFNAVGMKDSFQTSIVLGIVNFASTFVALYTVDKFGRRKCLLGGSASMAICF
VIFSTVGVTSLYPNGKDQPSSKAAGNVMIVFTCLFIFFFAISWAPIAYVIVAESYPLR
VKNRAMAIAVGANWIWGFLIGFFTPFITSAIGFSYGYVFMGCLVFSFFYVFFFVCETK
GLTLEEVNEMYVEGVKPWKSGSWISKEKRVSEE"
gene 270180..270251
/locus_tag="P301_M11126"
tRNA 270180..270251
/locus_tag="P301_M11126"
/product="tRNA-Glu"
/note="TE(UUC)M"
gene 270513..274345
/gene="Clu1"
/locus_tag="P301_M11131"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(274518..276113)
/gene="Sec59"
/locus_tag="P301_M11136"
mRNA complement(274518..276113)
/gene="Sec59"
/locus_tag="P301_M11136"
/product="Sec59p"
CDS complement(274518..276113)
/gene="Sec59"
/locus_tag="P301_M11136"
/note="Similar to YMR013C 'Dolichol kinase, catalyzes the
terminal step in dolichyl monophosphate (Dol-P)
biosynthesis; required for viability and for normal rates
of lipid intermediate synthesis and protein
N-glycosylation'"
/codon_start=1
/product="Sec59p"
/protein_id="EWG89319.1"
/translation="MVAIIPHASFTTIKLTQKTEGSQMPTEEICKINMRTRKFDVGGN
SRDFECFYSNFVQTVILLGTFFYCVERLQPWSIVTADISYKQIFVNVFVVCLIMVGLI
FTKYWQHGYKSLPKFDTIYSLYLPFMVSLLFDTSSTVINTILILSVLNAYRWRTQLVV
IILQLCLIFFNFEAGDRLKNIISIVINSLLSLILKYIGQLKSLDNIDSNLFSILLTNI
LYVSEAGTVHFRILKGIILALTTIISINYVLKKVMHFKPFMLSISFAIGLPLFANTFI
HLEDGENPLLWLVKYILESTIRQKILFAWSSILILSIPSILIEKDSLSLNTSRKLWHF
IIFLLIIPSFQMDSNFVKIALSGTIPVFLSIEYIRFQNLPPLGSAIELQLRRFADDRD
HSGPLIISYLYLLFGISTPLLMNNSPMGLIGLGIGDSLASIIGKRYGRIRWKGTQKTL
EGTLAFIVTSFIVCLILLRFDKAAIFNHLTTLQLLTLCTLSGVLEGNSVLNDNILIPA
FMMICEKINYSLTKSIIFNFKIT"
gene 276491..276903
/locus_tag="P301_M11146"
ncRNA 276491..276903
/ncRNA_class="snoRNA"
/locus_tag="P301_M11146"
/product="SNR78"
assembly_gap 276555..276751
/estimated_length=197
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 276882..276969
/locus_tag="P301_M11156"
ncRNA 276882..276969
/ncRNA_class="snoRNA"
/locus_tag="P301_M11156"
/product="SNR77"
gene 277101..277209
/locus_tag="P301_M11161"
ncRNA 277101..277209
/ncRNA_class="snoRNA"
/locus_tag="P301_M11161"
/product="SNR76"
gene 277294..277382
/locus_tag="P301_M11166"
ncRNA 277294..277382
/ncRNA_class="snoRNA"
/locus_tag="P301_M11166"
/product="SNR75"
gene 277515..277602
/locus_tag="P301_M11171"
ncRNA 277515..277602
/ncRNA_class="snoRNA"
/locus_tag="P301_M11171"
/product="SNR74"
gene 277684..277789
/locus_tag="P301_M11176"
ncRNA 277684..277789
/ncRNA_class="snoRNA"
/locus_tag="P301_M11176"
/product="SNR73"
gene 277689..277769
/locus_tag="P301_M11181"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 277931..278028
/locus_tag="P301_M11186"
ncRNA 277931..278028
/ncRNA_class="snoRNA"
/locus_tag="P301_M11186"
/product="SNR72"
gene 278245..279804
/gene="Bud22"
/locus_tag="P301_M11191"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(280248..281864)
/gene="Erg5"
/locus_tag="P301_M11196"
mRNA complement(280248..281864)
/gene="Erg5"
/locus_tag="P301_M11196"
/product="Erg5p"
CDS complement(280248..281864)
/gene="Erg5"
/locus_tag="P301_M11196"
/note="Similar to YMR015C 'C-22 sterol desaturase, a
cytochrome P450 enzyme that catalyzes the formation of the
C-22(23) double bond in the sterol side chain in
ergosterol biosynthesis; may be a target of azole
antifungal drugs'"
/codon_start=1
/product="Erg5p"
/protein_id="EWG89320.1"
/translation="MSSVAENIIQHATHNSTLHQLAKDQPSVGVTTAFSILDTLKSMS
YLKIFATLICILLVWDQVAYQIKKGSIAGPKFKFWPIIGPFLESLDPKFEEYKAKWAS
GPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT
DYRKSLNGLFTKQALAQYLPSLEQIMDKYMDKFVRLSKENNYEPQVFFHEMREILCAL
SLNSFCGNYITEDQVRKIADDYYLVTAALELVNFPIIIPYTKTWYGKKTADMAMKIFE
NCAQMAKDHIAAGGKPVCVMDAWCKLMHDAKNSNDDDSRIYHREFTNKEISEAVFTFL
FASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMV
IKETLRYRPPVLMVPYVVKKNFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDEFIP
ERWVEGSKASEAKKNWLVFGCGPHVCLGQTYVMITFAALLGKFALYTDFHHTVTPLSE
KIKVFATIFPKDDLLLTFKKRDPITGEVFE"
gene complement(282615..284973)
/gene="Sok2"
/locus_tag="P301_M11201"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 283493..283679
/estimated_length=187
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 286868..288061
/gene="Spo20"
/locus_tag="P301_M11221"
mRNA 286868..288061
/gene="Spo20"
/locus_tag="P301_M11221"
/product="Spo20p"
CDS 286868..288061
/gene="Spo20"
/locus_tag="P301_M11221"
/note="Similar to YMR017W 'Meiosis-specific subunit of the
t-SNARE complex, required for prospore membrane formation
during sporulation; similar to but not functionally
redundant with Sec9p; SNAP-25 homolog'"
/codon_start=1
/product="Spo20p"
/protein_id="EWG89321.1"
/translation="MGFRKILASKSHHSRHHNQHHKNLKLQNHRYVLISNITGSHETK
YLSPFRMDNCSGSRRRDRLHVKLKSLRNKIHKQLHPNCRFDDATKTSDDKCVSYEVPE
KDGLATISLEEVFPKSNRCQIPEENLGETDSVIHRDLGNFANENDYPQWRKVESQYNL
ENVQPEEDEIVDRLRSEIRSTKLKSVKTTSRTLEKAIEARCTGKRVLQQLSCQSNQLT
KIESNCDMLKIQSNVADRKIDELAHENRSLLALKSPNPFRKKREREKRDQINNLKLKH
RHLQQETMKRAQDSDKNLAINLSSEYGRYGQGVERQRILRDAQKYQFEADEEDNQMEI
DLYGNLEQIKAVSGDLKIMAHAFGREFEAQNTRMFDIENNVQQADNALQAKRYRLEKV
IGKRW"
gene 289583..291127
/locus_tag="P301_M11226"
mRNA 289583..291127
/locus_tag="P301_M11226"
/product="hypothetical protein"
CDS 289583..291127
/locus_tag="P301_M11226"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89322.1"
/translation="MNEVTCSITGDNPIHKINNGLGLKWNNLGKFSDFQTNDSAARDA
RTIDYIFTNCQTGSSIGKIDFRAALPADKSQHSGVSEKEFSRLENQWSKEFSCFPKNK
NADVTKPSRNKHEKRSANLHNRYFAQYYSTAYQQNRIYPCRISYNEHSSVSNGWEFQF
KSIENQLLNELKIENNVEEKTVGYEYVAEYEETIDFMHMLSSVPQTYQFLKSNIYITE
RDPYKIGCVLMDNGSNLNEVVMAFEAAISQDPSHINAWLKLGIVNFENESESNGELAL
RNCLNLDPNNTIALENLAIHHINQQNESESLKLFHKWILSKFSKVFQPSAGENKDSIN
KIPKKAHLVHILESLLNMGIEKKDQYDIYSVLSILYYSDQKIKQSQKCLEFLLLEKPN
NGTIWNRYGAILANTKSYHSAINAYNKCKQLRPNFTRVRYNLAIAYMNKGDYVKASKM
LIEVILLRSKGYEHNKAKMQNKFMQNLKNALIASKNFDSLDLINGSHNTESLISTLKA
IYNKMD"
gene 291543..294380
/gene="Stb4"
/locus_tag="P301_M11236"
mRNA 291543..294380
/gene="Stb4"
/locus_tag="P301_M11236"
/product="Stb4p"
CDS 291543..294380
/gene="Stb4"
/locus_tag="P301_M11236"
/note="Similar to YMR019W 'Protein that binds Sin3p in a
two-hybrid assay; contains a Zn(II)2Cys6 zinc finger
domain characteristic of DNA-binding proteins;
computational analysis suggests a role in regulation of
expression of genes encoding transporters'"
/codon_start=1
/product="Stb4p"
/protein_id="EWG89323.1"
/translation="MKIYYILVFEHRSVAIKLIIVVIVLLQFFLARSRQIDRTWAHTN
RKERFREMTAIGNTDDALDTSTAASKENGKGRLRVQKACELCKKRKVKCDGNNPCLNC
SKHQKECRYDFKATNRKRRRRQVASAVRDVSKTYAETSESFPRDLLSKSNIIINAPSD
GVSSSASNSPNPNSHYHHISSTLPFMSGRPNHTFHSGSNLNGENNNNSFPEDHMAKLL
LQLSSKLGNTTKESSIRTTRTNASDVNANPTVVNMKNSQEDCDTNHRSAICDSAEALH
NNNINSKENKIINSQITNTVNDHFESPWQTFSLDKYRFHRRYQNILPYYLGVSILKDL
SPQTIEYAKLKRPRVQNYGWNLSGGHYLKYKGDFRSQEKNIRHESKFFDFDDPVHLSL
INKLLRYYFDEINPVFSIIHEATFWQQYNNKFLRQGKQNNSSANLFTSMLYLILSTTL
RFREGHLDGQKGQGTYSNTSLNITFEEKSILIKKPSIEENLFKYAYLIINTLTFEWES
FELIQSWLLITFYFRTCYRQTACWNALSQAVNMCNGMSLYLNKFPEIHSTYDESKAWH
CFWCCFIMDKLISFQMGRFYQLSLPASEMCEQMNLVKSKKFLQEEDDWFHEETFQMLD
LSIIVTQFLKRDAQDLNLNETVQLRSQLGQWYDTFIVGSQTNAYDDNYRYFYQVQPFM
TYLDIRLTFEVRQLFCLIAPSSTANNKSLEYVVDTELLISHCQMAIENLAEITRSNLF
FVPWWLNLSQLFTVNLICIIYLHAGIAVTQNKAIMQSCQEIWRTLECSKPKNRPSMLP
ECLWCLKMLNHMFCIRLRDSALQLEATLGTDHGDDTPNRNKFEQFKKVGDNDADVEVD
AGEREENADERQENPHNNSKRVPLATRSHNTTNFDGSIAISPESAVANLGTDTGLPSD
VLDTVSKIGNSPNVFDDDLFSNLLWFDQNFA"
gene 295114..296277
/gene="Fms1"
/locus_tag="P301_M11241"
mRNA 295114..296277
/gene="Fms1"
/locus_tag="P301_M11241"
/product="Fms1p"
CDS 295114..296277
/gene="Fms1"
/locus_tag="P301_M11241"
/note="Similar to YMR020W 'Polyamine oxidase, converts
spermine to spermidine, which is required for the
essential hypusination modification of translation factor
eIF-5A; also involved in pantothenic acid biosynthesis'"
/codon_start=1
/product="Fms1p"
/protein_id="EWG89324.1"
/translation="MSKFAELEFHQHLGVSDCSFFQLVMKYLLQRRQFLTNDQIRYLP
QLCRYLELWHGLDWKLLSAKDTYFGHQGRNAFALNYDSVVQRIAQSFPQNWLKLSCEV
KSITREPSKNVTVNCEDGTVYNADYVIITVPQSVLNLSVQPEKNLRGRIEFQPPLKPV
IQDAFDKIHFGALGKVIFEFEECCWSNESSKIVTLANSTNEFVEIVRNAENLDELHSM
LEREDSQKHTSVTCWSQPLFFVNLSKSTGVASFMMLMQAPLTNHIESIREDKERLFSF
FQPVLNKIMKCLDSEDVIDGMRPIENIANANKPVLRNIIVSNWTRDPYSRGAYSACFP
GDDPVDMVVAMSNGQDSRIRFAGEHTIMDGAGCAYGAWESGRREATRISDLLK"
gene complement(296539..297792)
/gene="Mac1"
/locus_tag="P301_M11251"
mRNA complement(296539..297792)
/gene="Mac1"
/locus_tag="P301_M11251"
/product="Mac1p"
CDS complement(296539..297792)
/gene="Mac1"
/locus_tag="P301_M11251"
/note="Similar to YMR021C Copper-sensing transcription
factor involved in regulation of genes required for high
affinity copper transport"
/codon_start=1
/product="Mac1p"
/protein_id="EWG89325.1"
/translation="MIIFNGNKYACASCIRGHRSSTCRHSHRMLIKVRTRGRPSPMAI
RDAILVDSTSQSTEYENGAQIEGDCCSAMNQQPILFVRASAVRKARMINGKLHILMEE
GFTAHEPKDISTFTDDGNKYITETEFLRKHSPKAPATGTISPDSTKSSSSSEKKERSR
LQQEPIRHFSNCCKKDKSQNPASNGKTNKAPSDDIFTPYGSLESTSAFNDILQENYNS
SVPGAHDSSETLTPQSTTTIAAPHSSDVASKVEVLTHKGIFLSTQCSCEDESCPCVNC
LIHRSEEELNSYIQQSGVPLTNIGEAQITDKMMDYLDDCKCTDKECICPPDNCTCDGC
FSHSTNIIPFEKFFFYGILNARLTRKTQIKFKGKLVPSKYWWDFLKLQVPLMTDAQLE
LLDIHAWFQKLVSNYAPHLSDATTS"
gene 298054..298551
/gene="Ubc7"
/locus_tag="P301_M11256"
mRNA 298054..298551
/gene="Ubc7"
/locus_tag="P301_M11256"
/product="Ubc7p"
CDS 298054..298551
/gene="Ubc7"
/locus_tag="P301_M11256"
/note="Similar to YMR022W 'Ubiquitin conjugating enzyme,
involved in the ER-associated protein degradation pathway;
requires Cue1p for recruitment to the ER membrane;
proposed to be involved in chromatin assembly'"
/codon_start=1
/product="Ubc7p"
/protein_id="EWG89326.1"
/translation="MSKTAQKRLLKELQQLIKDSPPGIVAGPKSENNIFIWDCLIQGP
PDTPYADGVFNAKLEFPKDYPLSPPKLTFTPSILHPNIYPNGEVCISILHSPGDDPNM
YELAEERWSPVQSVEKILLSVMSMLSEPNIESGANIDACILWRDNRPEFERQVKLSIL
KSLGF"
gene complement(298811..300389)
/gene="Mss1"
/locus_tag="P301_M11261"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(300520..300592)
/locus_tag="P301_M11271"
tRNA complement(300520..300592)
/locus_tag="P301_M11271"
/product="tRNA-Ala"
/note="tRNA-Ala (Ala1);
TA(AGC)M1"
gene 301248..302420
/gene="Mrpl3"
/locus_tag="P301_M11281"
mRNA 301248..302420
/gene="Mrpl3"
/locus_tag="P301_M11281"
/product="Mrpl3p"
CDS 301248..302420
/gene="Mrpl3"
/locus_tag="P301_M11281"
/note="Similar to YMR024W Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl3p"
/protein_id="EWG89327.1"
/translation="MGIVLKRAIAAGMKPFPNSTWHWSRTIRPFSQHLSSTCFLQQSS
RFTSKRYLHLSTLTQQEKRFLPESELAKYKEYYQGLKSTVNEIPESVASKSPSLRTLH
KRLQLPNELTYSTLSRCLTCPSAKLPDKINNPTKGAAFVNTVPTNKYLDNHGLNIMGK
NLLSYHVTKSIIQKYPRLPTVVLNAAVNAYISEAVLAHIAKYWGIEVETTSVLSRYLK
MEPFEFTLGRLKFFNNSLNSKDGIELITGKNFSETSALAMSVRSIIAAIWAVTEQKDS
QAVYRFIDDHIMSRKLDITKMFQFEQPTRELAMLCRREGLEKPVSKLVAESGRLSKSP
VFIVHVFSGEETLGEGYGSSLKEAKARAATDALMKWYCYEPLAQQEPVIDPGTVVV"
gene 302671..303558
/gene="Csi1"
/locus_tag="P301_M11291"
mRNA 302671..303558
/gene="Csi1"
/locus_tag="P301_M11291"
/product="Csi1p"
CDS 302671..303558
/gene="Csi1"
/locus_tag="P301_M11291"
/note="Similar to YMR025W 'Subunit of the Cop9
signalosome, which is required for deneddylation, or
removal of the ubiquitin-like protein Rub1p from Cdc53p
(cullin); involved in adaptation to pheromone signaling'"
/codon_start=1
/product="Csi1p"
/protein_id="EWG89328.1"
/translation="MDLLKFSSLAISEINFLHESSFDSIDHSWFLLIGCKLDQDDEIY
IPTNGNEAESQWYIEKVIRIPMQENDKINQERLERRINLTKVTQKDICILGILDLCQL
EEDENITNKVTEKVLTQLTALALKYLIKYNVFRQHTSFQEAVNSLKGYKIENSVQIGA
EIILDFLQDKVQIKDVNDRYQIPTPNNTVDPGFDEFQLIDMKDKEINIQKYNNNTIRK
LLEKINRMIIFLKNYDATDKPFSSTQDVILRKISMLVTQLQRGGTSDMNYLLDNKINE
IKLLEISCKQWEISNMLKK"
gene complement(303607..304806)
/gene="Pex12"
/locus_tag="P301_M11296"
mRNA complement(303607..304806)
/gene="Pex12"
/locus_tag="P301_M11296"
/product="Pex12p"
CDS complement(303607..304806)
/gene="Pex12"
/locus_tag="P301_M11296"
/note="Similar to YMR026C 'C3HC4-type RING-finger peroxin
and E3 ubiquitin ligase, required for peroxisome
biogenesis and peroxisomal matrix protein import; forms
translocation subcomplex with Pex2p and Pex10p; mutations
in human homolog cause peroxisomal disorder'"
/codon_start=1
/product="Pex12p"
/protein_id="EWG89329.1"
/translation="MSFYSNLPSAGQSSRGSSTSGRNGVGLEPLYPTIFEIMSSQEID
SLLPASIRYLLANHLVANFPNRYTLRLNKYFFEWFQAIKGFVEWYHLKTYNSTFIDRF
YGLQLFSSRDRNLALTQCLNPKGQSEWPQGLQLNQQQKSVIFLEKIILPYITAKLDEI
LEKISMNNIFSSDETENKWPKRAFLRIYPFIKKLLALSNLLVKLLFLTKRTGSVSLLQ
YLFKIEYTTVRPLSSELSGLKETKGMDNRLRKTNISSIFALMQGQLSIIPRFLTFMGS
QFFPTFIFVLRVYQWWTTQDMTTKLQKRVNDLDEDIPRPPFSSHSDKTEDKEGVSEAC
PVCEKTVQNPCVLETGYVACYPCAISYLVNNEGHCPVTNKKLLGCTYNKHTNKWEVVT
GIRKLLI"
gene 305248..306660
/locus_tag="P301_M11301"
mRNA 305248..306660
/locus_tag="P301_M11301"
/product="hypothetical protein"
CDS 305248..306660
/locus_tag="P301_M11301"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89330.1"
/translation="MTIPGRFMTNDKGTFGEYTASTRWPIIIQNAIDDLSKHQETEKS
NGTKFEQGEVIKKELKEFRQEIIDRVPLRPFTEEEIKIANVPLSFNEYLKKHPEVNWG
AVEWLFSEVYLYRRVNVLFQRQCEWAKFDIFNRLKQSTFESSFYGVVELALRYGNLLP
QLREMKQNPGNEIDDILKVLFKEFIEISLWGNATDLSLLTNATLEDIKSIQGAKARAA
SESKIVVNDTEKAWEVLTKARADANSREIRVDFVLDNSGFELYADLMLAAFLLQSGLA
TKCIFHAKDIPYMVSDVMLKDFDILIHDLRDREFFPSGEPSTKESRALDLFAGEMEKF
VSSGKIEFREDSFWTTELDYWNLDANETKYHGSILHKDLQKSNLVIFKGDLNYRKLTG
DRKWPRTTKWETAIGPLATNGITSLSLRTCKADVQVALPEGLDAKLSQEWEKENPGRG
SWWCCSGKWAVICFCSGIHK"
gene 306853..307953
/gene="Tap42"
/locus_tag="P301_M11306"
mRNA 306853..307953
/gene="Tap42"
/locus_tag="P301_M11306"
/product="Tap42p"
CDS 306853..307953
/gene="Tap42"
/locus_tag="P301_M11306"
/note="Similar to YMR028W 'Essential protein involved in
the TOR signaling pathway; physically associates with the
protein phosphatase 2A and the SIT4 protein phosphatase
catalytic subunits'"
/codon_start=1
/product="Tap42p"
/protein_id="EWG89331.1"
/translation="MASVTEQFNDIISLYSTKLEHTSLRQDSPEYQGLLLSTIKKLLN
LKTAIFDRLALFSTNETIDDVSTASIKFLAVDYYLGLLISRRQSNDSDVAQRQSMKLI
YLKKSVESFINFLTLLQDYKLLDPLVGEKLGNFKDRYNPQLSELYAQPKNDKDLSGAQ
LKRKEKIELFQRNKEISTKLHCLELELKNNDEDHDHDELLRELYLMRLHHFSLDTINN
IEQNLFECEMLSNFLKNSVHEVKSSGTQIRKESNDDDSTGFTDKLENINKPLIDKKGQ
VLRNFTLVDKRQQLQQKVRGYGQYGPTMSVEEFLDKEFEEGRVLQGGEEPEQAPDEEN
MDWQDRETYKAREWDEFKESHAKGSGNTMNRG"
gene complement(308031..309603)
/gene="Far8"
/locus_tag="P301_M11311"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 308262..308272
/estimated_length=11
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 310164..311295
/gene="Rsf1"
/locus_tag="P301_M11321"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(311588..314119)
/locus_tag="P301_M11326"
mRNA complement(311588..314119)
/locus_tag="P301_M11326"
/product="hypothetical protein"
CDS complement(311588..314119)
/locus_tag="P301_M11326"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89332.1"
/translation="MSLISAVEDRDIHNIGKTSGGGSRTSSITSSKKSLKHGSKSLRK
PKVYQTTGEPLSREALYKAKLKYGVYQSPAQSYSIGVSDAHAASDKAANLAHDNQTTV
EAYKRMFIDPNATKAASKMGPKVVRNNSITSATSKTSKESQTKRKSKESPGAAASKAY
SMTMETTSLSSQTNSRSYSITSASSVLSGASGSFNSTVNPKPKTLNLEKVLVGAEKKA
ESRIKERWEPEKTNFQYGVKTDEHGNLNQFSFSNEMMNNIMAKVDAPKAQDLQKVKKV
SAEKEAKSMKFALGAANAVKDMHPGEDIDKSIALKAQKRETYLSQLTSQQVLTLARAN
VDRQLDIIEKSDMHRKLFTNMEYNKAAVAVAQSNHQKKTEFHNKINMGGGLFLSPEDI
TKIASGLISPVLGEVSERAEAQRAMDEEIAERTEAYNKSLNEWETMERSIISNDAKVL
TTTANRHQTEKKTSQEKIKASFDALVARMDTKVAERETLLEDTKSKEIEFKKQMQQEL
KDEKARLDQDLEEWGKKCEQDITEARKEQEELLKPYHDDLANAEAEHKTLVEERDEIN
AEISRLQDAIVDHKRKISGYGNDLDAQKNRNIREDDKLLELGQTKESLESHLNDDVII
LANKAKEQAELSTKEARLKQLEVDSLINERKSELNATEIELKKEKLNLLEAMKDVASA
RGDDKIDEEKVKKLIGMTSEEYLTQNKSVEKNVEDLPTQLEKIEEGDELKKEEIVGAE
TKNSGGDGVSVSTAAKEATETSSAVQTKEPEEKISIGNKSSGKEDANDCKSAEHSKEI
SVSQKAGNNKSLGVSPDSLEHTFSGFSQGSSIEDDQDAISNQEKK"
gene 314085..314411
/locus_tag="P301_M11331"
mRNA 314085..314411
/locus_tag="P301_M11331"
/product="hypothetical protein"
CDS 314085..314411
/locus_tag="P301_M11331"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89333.1"
/translation="MSLSSTAEIKDIGYFALVPYMQSYLTTKDCVIVRVHYFASCQSS
CQRAEPLGSTELINIGDILQVTRSSPHRVAVVLPPLEGAPFGSLRFSSRRAPIAPSGK
YYFPFG"
gene 314674..316683
/gene="Hof1"
/locus_tag="P301_M11336"
mRNA 314674..316683
/gene="Hof1"
/locus_tag="P301_M11336"
/product="Hof1p"
CDS 314674..316683
/gene="Hof1"
/locus_tag="P301_M11336"
/note="Similar to YMR032W 'Bud neck-localized, SH3
domain-containing protein required for cytokinesis;
regulates actomyosin ring dynamics and septin
localization; interacts with the formins, Bni1p and Bnr1p,
and with Cyk3p, Vrp1p, and Bni5p'"
/codon_start=1
/product="Hof1p"
/protein_id="EWG89334.1"
/translation="MSYSYEACFWDPNDNGVNILLGHISQGIKSCDSMIHFFKQRSEL
EKDYARRLGAITGKLDKDIGTNMDYGKLNETFNVVLSVEKARAQSHSKQSEILFRQIY
TDTKAFAANLQARYTTLSGKIERLRMDKFNKKKGCEVLQKKLQDAQIRFRDLQLNENN
MIGAKRVEHNKRELLKWESNSQEYKVQLDVLKQEYKASQKFWIHEWAQLSCELQEMEN
ARISFLQSKLQQFATSSMETYILEQTKMDMLTNHLNSFTAADEISTFSKENGTGRLKH
KTSKGDMNSSANWAQMSSISTTSKKTESYMDNIRKLSSQLKETENKRKLASIDKYEKP
LPSPEVTMATQFRNSTPVIRNETKVVADPTLSLRSSPVQLQSNVDDSVLRQKPDKPRP
IVGEEQLKPDEDSKNPDEKGLMVHKRNQSLSSPSESSSSNPTDFSHIKKRQSMESMTT
SVSSMANSIDDSQRFAKSWNSSNRKRKSMSHLQVPSSASSRSDDGGRTPNSEHNLNED
DYNTRRDTSTSTILFKPPVAVRGTSRGHTHRQSMIMQDSSNPIEDALYEMERIQSSSK
PGTKTGNIMDERGVVRDRGITVTLPIVTSEGFPVIEYAKAMYPLIGNEAPGLANFHKG
DYLLITEIVNKDWYKGEVYDNDRIDRNHRIGLIPYNFIQLLHQGL"
gene 316689..316981
/locus_tag="P301_M11341"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 316796..316818
/estimated_length=23
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 317166..318655
/gene="Arp9"
/locus_tag="P301_M11346"
mRNA join(317166..317195,317282..318655)
/gene="Arp9"
/locus_tag="P301_M11346"
/product="Arp9p"
CDS join(317166..317195,317282..318655)
/gene="Arp9"
/locus_tag="P301_M11346"
/note="Similar to YMR033W 'Component of both the SWI/SNF
and RSC chromatin remodeling complexes; actin-related
protein involved in transcriptional regulation'"
/codon_start=1
/product="Arp9p"
/protein_id="EWG89335.1"
/translation="MAPFRQDSILIIYPRSQTTLVQFGLNEETFTVPELEIPTQIYRT
TRQDGSYTYHSTNKDNKAELIKPIQNGEIIDISAFTQFLRLIFVSILSDRANKNQDAF
EAELSNIPLLLITHHSWSQSDLEIITQYVFESLEINNLIQLPASLAATYSMISLQNCC
IIDVGTHHTDIIPIVDYAQLDHLVSSIPMGGQSINDSLKKLLPQWDDDQIESLKKSPI
FEVLSDDAKKLSSFDFGNENEDEDEGTLNVAEIITSGRDTREVLEERERGQKVKNVKN
SDLEFNTFWDEKGNEIKVGKQRFQGCNNLIKNISNRVGLTLDNIDDINKAKAVWENII
IVGGTTSISGFKEALLGQLLKDHLIIEPEEEKSKREEEAKSVLPAATKKKSKFMTNST
AFVPTIEYVQCPTVIKLAKYPDYFPEWKKSGYSEIIFLGAQIVSKQIFTHPKDTFYIT
REKYNMKGPAALWDVQF"
gene complement(318796..320097)
/locus_tag="P301_M11351"
mRNA complement(318796..320097)
/locus_tag="P301_M11351"
/product="hypothetical protein"
CDS complement(318796..320097)
/locus_tag="P301_M11351"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89336.1"
/translation="MKTQYSLIRKIWAHSVTEFLKSQWFFICLAILIVIARFAPNFAR
DGGLIKGQYSIGYGCVAWIFLQSGLGMKSRSLMANMLNWRAHATILVLSFLITSSIVY
GFCCAVKAANDPKIDDWVLIGLILTATCPTTVASNVIMTTNAGGNSLLCVCEVFIGNL
LGAFITPALVQMFTNRAPFAYGNPATGNGIGALYGRVMKQVGLSVFVPLFVGQVIQNC
FPKGTAYYLGFLKKYHIKIGSYMLLLIMFSSFSTAFYQDAFTSVSHVCIIFLCFFNLG
IYIFFTGLSYLCARPWFILKLFPHEPIEGKSTRLYRYSYNIFRPFYYSKEDAICIMFC
GPAKTAALGVSLITSQYGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLSNNVPYTQPR"
assembly_gap 318837..319039
/estimated_length=203
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 320517..321050
/gene="Imp2"
/locus_tag="P301_M11356"
mRNA 320517..321050
/gene="Imp2"
/locus_tag="P301_M11356"
/product="Imp2p"
CDS 320517..321050
/gene="Imp2"
/locus_tag="P301_M11356"
/note="Similar to YMR035W 'Catalytic subunit of the
mitochondrial inner membrane peptidase complex, required
for maturation of mitochondrial proteins of the
intermembrane space; complex contains Imp1p and Imp2p
(both catalytic subunits), and Som1p'"
/codon_start=1
/product="Imp2p"
/protein_id="EWG89337.1"
/translation="MFRAGSSKRFLRNTLIAISWVPVLLTINNNVVHIAQVKGTSMQP
TLNPQTETLATDWVLLWKFGVKNPSNLSRDDIILFKAPTNPRKVYCKRVKGLPFDTID
TKFPYPKPQVNLPRGHIWVEGDNYFHSIDSNTFGPISSGLVIGKAITIVWPPSRWGTD
LKLSTGRDCISKRAILE"
gene complement(321231..322895)
/gene="Mih1"
/locus_tag="P301_M11361"
mRNA complement(321231..322895)
/gene="Mih1"
/locus_tag="P301_M11361"
/product="Mih1p"
CDS complement(321231..322895)
/gene="Mih1"
/locus_tag="P301_M11361"
/note="Similar to YMR036C 'Protein tyrosine phosphatase
involved in cell cycle control; regulates the
phosphorylation state of Cdc28p; homolog of S. pombe
cdc25'"
/codon_start=1
/product="Mih1p"
/protein_id="EWG89338.1"
/translation="MNNIFHGTEDECANEDVLSFQKISLKSPFGKKKNIFRNVQTFFK
SKSKHSNVDDDLINKENLAFDKSPLLTNHRSKEIDGPSPNIKQLGHRDELDENENEND
DIVLSMHFASQTLQSPTRNSSRRSLTNNRDNDLLSRIKYPGSPQRSSSFSRSRSLSRK
PSMNSSSNSSRRVQRQDGKIPRSSRKSSQKFSNITQNTLNFTSASSSPLAPNSVGVKC
FESCLAKTQIPYYYDDRNSNDFFPRISPETLKNILQNNMCEPFYNSCRIIDCRFEYEY
TGGHIINSVNIHSRDELEYEFIHKVLHSDTSNNNTLPTLLIIHCEFSSHRGPSLASHL
RNCDRIINQDHYPKLFYPDILILDGGYKAVFDNFPELCYPRQYVGMNSQENLLNCEQE
MDKFRRESKRFATKNNSFRKLASPSNPNFFYRDSHQSSTTMASSALSFRFEPPPKLSL
NHRRVSSGSSLNSSESTGDENFFPILSKSSMSSNSNLSTSHMLLMDGLDTPSYFSFED
ERGNHQQVSGDEEQDGDFTFVGSDREDLPRPARRSLFPSLETEDKK"
gene complement(323778..325892)
/gene="Msn2"
/locus_tag="P301_M11371"
mRNA complement(323778..325892)
/gene="Msn2"
/locus_tag="P301_M11371"
/product="Msn2p"
CDS complement(323778..325892)
/gene="Msn2"
/locus_tag="P301_M11371"
/note="Similar to YMR037C 'Transcriptional activator
related to Msn4p; activated in stress conditions, which
results in translocation from the cytoplasm to the
nucleus; binds DNA at stress response elements of
responsive genes, inducing gene expression'"
/codon_start=1
/product="Msn2p"
/protein_id="EWG89339.1"
/translation="MTVDHDFNSEDILFPIESMSSIQYVENNNPNNINNDVIPYSLDI
KNTVLDSADLNDIQNQETSLNLGLPPLSFDSPLPVTETIPSTTDNSLHLKADSNKNRD
ARTIENDSEIKSTNNANGSGANQYTTLTSPYPMNDILYNMNNPLQSPSPSSVPQNPTI
NPPINTASNETNLSPQTSNGNETLISPRAQQHTSIKDNRLSLPNGANSNLFIDTNPNN
LNEKLRNQLNSDTNSYSNSISNSNSNSTGNLNSSYFNSLNIDSMLDDYVSSDLLLNDD
DDDTNLSRRRFSDVITNQFPSMTNSRNSISHSLDLWNHPKINPSNRNTNLNITTNSTS
SSNASPNTTTMNANADSNIAGNPKNNDATIDNELTQILNEYNMNFNDNLGTSTSGKNK
SACPSSFDANAMTKINPSQQLQQQLNRVQHKQLTSSHNNSSTNMKSFNSDLYSRRQRA
SLPIIDDSLSYDLVNKQDEDPKNDMLPNSNLSSSQQFIKPSMILSDNASVIAKVATTG
LSNDMPFLTEEGEQNANSTPNFDLSITQMNMAPLSPASSSSTSLATNHFYHHFPQQGH
HTMNSKIGSSLRRRKSAVPLMGTVPLTNQQNNISSSSVNSTGNGAGVTKERRPSYRRK
SMTPSRRSSVVIESTKELEEKPFHCHICPKSFKRSEHLKRHVRSVHSNERPFACHICD
KKFSRSDNLSQHIKTHKKHGDI"
gene complement(326885..327634)
/gene="Ccs1"
/locus_tag="P301_M11386"
mRNA complement(326885..327634)
/gene="Ccs1"
/locus_tag="P301_M11386"
/product="Ccs1p"
CDS complement(326885..327634)
/gene="Ccs1"
/locus_tag="P301_M11386"
/note="Similar to YMR038C 'Copper chaperone for superoxide
dismutase Sod1p, involved in oxidative stress protection;
Met-X-Cys-X2-Cys motif within the N-terminal portion is
involved in insertion of copper into Sod1p under
conditions of copper deprivation'"
/codon_start=1
/product="Ccs1p"
/protein_id="EWG89340.1"
/translation="MTTNDTYEATYAIPMHCENCVNDIKACLKNVPGINSLNFDIEQQ
IMSVESSVAPSTIINTLRNCGKDAIIRGAGKPNSSAVAILETFQKYTIDQKKDTAVRG
LARIVQVGENKTLFDITVNGVPEAGNYHASIHEKGDVSKGVESTGKVWHKFDEPIECF
NESDLGKNLYSGKTFLSAPLPTWQLIGRSFVISKSLNHPENEPSSVKDYSFLGVIARS
AGVWENNKRVCACTGKTVWEERKDALANNIK"
gene complement(327750..328832)
/gene="Sub1"
/locus_tag="P301_M11396"
mRNA complement(327750..328832)
/gene="Sub1"
/locus_tag="P301_M11396"
/product="Sub1p"
CDS complement(327750..328832)
/gene="Sub1"
/locus_tag="P301_M11396"
/note="Similar to YMR039C 'Transcriptional coactivator,
facilitates elongation through factors that modify RNAP
II; role in peroxide resistance involving Rad2p; role in
the hyperosmotic stress response through polymerase
recruitment at RNAP II and RNAP III genes'"
/codon_start=1
/product="Sub1p"
/protein_id="EWG89341.1"
/translation="MSYYNRYRNKRRSDNGGGNLSNSNNNNGGMPSGLSASDAIFDLG
KNKRVTVRQFRNINLIDIREYYLDSSTGEMKPGKKGISLTEDLYDELLKHRLNIDEAL
RRLGSKRPKTKMVRLLSDDEYEDDNNNDSTNNDKDKNGKDKNSPKKRREDKSKASNES
HDLEPRSKKKKPAPPTLLPHEENIQNAEREANATLIIPGQAGRKQQEERKQKEKEEAE
EAKAKAVAEQEKEAKAKEKIAEPEPEPVPTLQAKKEDIVXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVIYNNQTKVNLVHFS
IIYYQKNLRLLSIFPVQLVHSENGRG"
assembly_gap 327878..328060
/estimated_length=183
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 329690..330172
/gene="Yet2"
/locus_tag="P301_M11401"
mRNA 329690..330172
/gene="Yet2"
/locus_tag="P301_M11401"
/product="Yet2p"
CDS 329690..330172
/gene="Yet2"
/locus_tag="P301_M11401"
/note="Similar to YMR040W 'Protein of unknown function
that may interact with ribosomes, based on co-purification
experiments; homolog of human BAP31 protein'"
/codon_start=1
/product="Yet2p"
/protein_id="EWG89342.1"
/translation="MGVYLAVLFSLLVIEMAILFILVLPLPQRMRRWLYIRYSIISTN
KKFRTYMVGIMIFVGLLFIDSWKRSQIRVSTYRNQKNPYIINSVTPVDALASRAYNQR
NVYISGFIIYFYICILTVMSILRRIVEWNDKMKAGDDILKEKLRRKQKYLEELQKKKF
"
gene complement(330275..331282)
/gene="Ara2"
/locus_tag="P301_M11406"
mRNA complement(330275..331282)
/gene="Ara2"
/locus_tag="P301_M11406"
/product="Ara2p"
CDS complement(330275..331282)
/gene="Ara2"
/locus_tag="P301_M11406"
/note="Similar to YMR041C 'NAD-dependent arabinose
dehydrogenase, involved in biosynthesis of
dehydro-D-arabinono-1,4-lactone; similar to plant
L-galactose dehydrogenase'"
/codon_start=1
/product="Ara2p"
/protein_id="EWG89343.1"
/translation="MVNEKVNPFDLASVSPLVLGGAILNQQYTDEPESIPLEDIIKYA
FSHGINAIDTSPYYGPSEVLYGRALSNLRNEFPRDTYFICTKVGRIGAEEFNYSRDFV
RFSVHRSCERLHTTYLDLVYLHDVEFVKFPDILEALKELRTLKNKGVIKNFGISGYPI
DFITWLAEYCSTEESDIGSLDAVLSYCNLNLQNNKLLNFRERLLRNAKLKMVCNASIL
SMSLLRSQETRQFHPCSHELRECASQAAKYCQEQNVDLADLATRYAISEWVGKGPVVL
GVSSMEELKLALDNYEIVKSNGNRLSSKDGQLVEYIQKNIFKEHFNEEWSSGIPHPEM
I"
assembly_gap 331591..331692
/estimated_length=102
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 331912..332445
/gene="Arg80"
/locus_tag="P301_M11421"
mRNA 331912..332445
/gene="Arg80"
/locus_tag="P301_M11421"
/product="Arg80p"
CDS 331912..332445
/gene="Arg80"
/locus_tag="P301_M11421"
/note="Similar to YMR042W 'Transcription factor involved
in regulation of arginine-responsive genes; acts with
Arg81p and Arg82p'"
/codon_start=1
/product="Arg80p"
/protein_id="EWG89344.1"
/translation="MTSNSDGSSTSPVEKPITGDVETNEPTKPIRRLSTPSPEQDQEG
DFDEEDDDDKFSVSTSTPTPTITKTKDSSDTSTVTRRKQPIRYIENKTRRHVTFSKRR
HGIMKKAYELSVLTGANILLLILANSGLVYTFTTPKLEPVVREDEGKSLIRACINASD
TPDATDTSPAQEQSPAN"
assembly_gap 332605..333017
/estimated_length=413
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 333182..334042
/gene="Mcm1"
/locus_tag="P301_M11426"
mRNA 333182..334042
/gene="Mcm1"
/locus_tag="P301_M11426"
/product="Mcm1p"
CDS 333182..334042
/gene="Mcm1"
/locus_tag="P301_M11426"
/note="Similar to YMR043W 'Transcription factor involved
in cell-type-specific transcription and pheromone
response; plays a central role in the formation of both
repressor and activator complexes'"
/codon_start=1
/product="Mcm1p"
/protein_id="EWG89345.1"
/translation="MSDIEEGTPTNNGQQKERRKIEIKFIENKTRRHVTFSKRKHGIM
KKAFELSVLTGTQVLLLVVSETGLVYTFSTPKFEPIVTQQEGRNLIQACLNAPDDEEE
DEEEDGDDDDDDDDDGNDMQRQQPQQQQPQQQQQVLNAHANSLGHLNQDQVPAGALKQ
EVKSQLLGGANPNQNSMIQQQQHHTQNSQPQQQQQQQPQQQMSQQQMSQHPRPQQGIP
HPQQSQPQQQQQQQQQLQQQQQQQQQQPLTGIHQPHQQAFANAASPYLNAEQNAAYQQ
YFQEPQQGQY"
gene 334692..336128
/gene="Ioc4"
/locus_tag="P301_M11431"
mRNA 334692..336128
/gene="Ioc4"
/locus_tag="P301_M11431"
/product="Ioc4p"
CDS 334692..336128
/gene="Ioc4"
/locus_tag="P301_M11431"
/note="Similar to YMR044W Member of a complex (Isw1b) with
Isw1p and Ioc2p that exhibits nucleosome-stimulated ATPase
activity and acts within coding regions to coordinate
transcription elongation with termination and processing,
contains a PWWP motif"
/codon_start=1
/product="Ioc4p"
/protein_id="EWG89346.1"
/translation="MSEAIFQPTDIVLAKVKGFSAWPAMIIPNELIPDNILKTKPVSV
HKGKSGSDKKANEDIDADMESEARGREQSEEEEDIEDFGESEANPEKFIIYTPVLKFR
KNDTLKSTYCVKFFCDDSYIWVKPMDMKILTSEDCRKWLSGKQRKNKKLIPAYEMAMR
GKNGIDIWEFVEYGSYGKPDEEEYVEEEEEENEPEKKAIRPTRSSSRQRQKRASETEK
SEGGNSNKRKRVTRSTRQQAIDASEEEEEEEEEEEEKVQEAVRKRPQRTKTKKVVVSK
TKPNPKTKAKKEKPKPPKPIKYHFEDDEDWSIVGLGPQDLSIEKTMDPIAKKLSQKKN
LEKHVEIKLDLEDKLAGINKLLCDVLCSAINQAVSIKDDFEIILDELQIALDTRGSRN
EFITIFQSNNSLLLNFRILFNLRKRELNKWDLWDRFQDIFKHIYSYQFIPDTEDWQLE
QNMEIEEMDREKPSFSEDVKEEESKVGA"
assembly_gap 336546..336616
/estimated_length=71
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(336831..340172)
/locus_tag="P301_M11436"
mRNA complement(336831..340172)
/locus_tag="P301_M11436"
/product="hypothetical protein"
CDS complement(336831..340172)
/locus_tag="P301_M11436"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89347.1"
/translation="MFGVSRGAFPSATTQPFGSTGSTFGAQQQQQQPVANTSAFGLSQ
QTNTTQAPAFGNFGNQTSNSPFGMSGSTTANGTPFGQSQLTNNNASGSIFGGMGNNTA
LSAGSASVVPNSTAGTSIKPFTTFEEKDPTTGVINVFQSITCMPEYRNFSFEELRFQD
YQAGRKFGTSQNGTGTTFNNPQGTTNTGFGIMGNNNSTTSATTGGLFGQKPATGMFGT
GTGSGGGFGSGATNSTGLFGSSTNLSGNSAFGANKPATSGGLFGNTTNNPTNGTNNTG
LFGQQNSNTNGGLFGQQQNSFGANNVSNGGAFGQVNRGAFPQQQTQQGSGGIFGQSNA
NANGGAFGQQQGTGALFGAKPASGGLFGQSAGSKAFGMNTNSTGTTGGLFGQTNQQQS
GGGLFGQQGNSNAGGLFGQNNQSQNQSGLFGQQNSSNAFGQPQQQGGLFGSKPAGGLF
GQQQGASTFASGNAQNNSIFGQNHQQQQSTGGLFGQQNNQSQSQPGGLFGQTNQNNNQ
PFGQNGLQQPQQNNSLFGAKPTGFGNTSLFSNSTTNQSNGISGNNLQQQSGGLFQNKQ
QPASGGLFGSKPSNTVGGGLFGNNQVANQNNPASTSGGLFGSKPATGSLFGGTNSTAP
NASSGGIFGSNNASNTAATTNSTGLFGNKPVGAGASTSAGGLFGNNNNSSLNNSNGST
GLFGSNNTSQSTNAGGLFQNNTSTNTSGGGLFSQPSQPMAQSQNALQQQQQQQRLQIQ
NNNPYGTNELFSKATVTNTVSYPIQPSATKIKADERKKASLTNAYKMIPKTLFTAKLK
TNNSVIDKAQIKVDPNLSISIDKKNNQIAISNQQEENLDESILKASELLFNPDKRSFK
NLINNRKMLIASEEKNNGSQNNDMNFKSKSEEQETILGKPKMDEKETANGGERMVLSS
KNDGEDSATKHHSRNMDEENKENVADLQKQEYSEDDKKAVFTDVAEKDASFINENYYI
SPSLDTLSSYSLLQLRKVPHLVVGHKSYGKIEFLEPVDLAGIPLTSLGGVIITFEPKT
CIIYANLPNRPKRGEGINVRARITCFNCYPVDKSTRKPIKDPNHQLVKRHIERLKKNP
NSKFESYDADSGTYVFIVNHAAEQT"
gene complement(340172..342681)
/gene="Nup116"
/locus_tag="P301_M11441"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 340448..341401
/gene="Csm3"
/locus_tag="P301_M11446"
mRNA 340448..341401
/gene="Csm3"
/locus_tag="P301_M11446"
/product="Csm3p"
CDS 340448..341401
/gene="Csm3"
/locus_tag="P301_M11446"
/note="Similar to YMR048W 'Replication fork associated
factor, required for stable replication fork pausing;
component of the DNA replication checkpoint pathway;
required for accurate chromosome segregation during
meiosis'"
/codon_start=1
/product="Csm3p"
/protein_id="EWG89348.1"
/translation="MDQDFDSLLLGFNDSDSVQKDPTVPNGLDGSVVDPTIADPTAIT
ARKRRPQVKLTAEKLLSDKGLPYVLKNAHKRIRISSKKNSYDNLSNIIQFYQLWAHEL
FPKAKFKDFMKICQTVGKTDPVLREYRVSLFRDEMGMSFDVGTRETGQDLERQSPMVE
EHVTSAEERPIVADSFAQDKRNVNNVDYDNDEDDDIYHLSYRNRRGRVLDERGNNETV
PNNVVPPKEDLDALLKTFRVQGPVGLEENEKKLLLGWLDAHRKMEKGSMTEEDVQLIQ
SLEEWEMNDIEGKHTHYDLLPGGDEFGVDQDELDAMKEMGF"
gene complement(341561..343984)
/gene="Erb1"
/locus_tag="P301_M11451"
mRNA complement(341561..343984)
/gene="Erb1"
/locus_tag="P301_M11451"
/product="Erb1p"
CDS complement(341561..343984)
/gene="Erb1"
/locus_tag="P301_M11451"
/note="Similar to YMR049C 'Constituent of 66S
pre-ribosomal particles, forms a complex with Nop7p and
Ytm1p that is required for maturation of the large
ribosomal subunit; required for maturation of the 25S and
5.8S ribosomal RNAs; homologous to mammalian Bop1'"
/codon_start=1
/product="Erb1p"
/protein_id="EWG89349.1"
/translation="MMAKNNKTTEAKMSKKRAASEESDVEEDEDKLLSVDGLIDAEAS
ESDEDDDEYESAVEEKESSSDKEAQDDSDDDSDAELNKLLAEEEGDGEEDYDSSEFSD
DTTSLTDRLSGVKLQTIVDPNIYSKYADGSDRIIKPEINPVYDSDDSDAETQNTIGNI
PLSAYDEMPHIGYDINGKRIMRPAKGSALDQLLDSIELPEGWTGLLDKNSGSSLNLTK
EELELISKIQRNEQTDDSINPYEPLIDWFTRHEEVMPLTAVPEPKRRFVPSKNEAKRV
MKIVRAIREGRIIPPKKLKEMKEKEKTENYQYDLWGDSTETNDHVMHLRAPKLPPPTN
EESYNPPEEYLLSPEEKEAWENTEYSERERNFVPQKYSALRKVPGYGESIRERFERSL
DLYLAPRVRKNKLNIDPNSLIPELPSPKDLRPFPIRCSTIYAGHKGKVRTLSIDPSGL
WLATGSDDGTVRVWEILTGREVYRTTLIDDEENPDDHIECIEWNPDANNGILAVAVGE
NIHLIVPPIFGYDIENNGKTKIEDGFGYDTFGTVKKSNLEVNENGDGDEDGENESAKD
AVKKQVAQWNKPSQKQLEKDICITISCKKTVKKLSWHRKGDYFVTVQPDSGNTSVLIH
QVSKHLTQSPFKKSKGIIMDAKFHPFKPQLFVCSQRYVRIYDLSQQILVKKLLPGARW
LSKIDIHPRGDNLIASSFDKRVLWHDLDLASTPYKTLRYHEKAVRSVNFHKKLPLFSS
AADDGTIHVFHATVYDDMMKNPMIVPLKKLTGHKVINSLGVLDAIWHPREAWLFSAGA
DNTARLWTT"
assembly_gap 344019..344346
/estimated_length=328
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(345911..345984)
/locus_tag="P301_M11461"
tRNA complement(345911..345984)
/locus_tag="P301_M11461"
/product="tRNA-Val"
/note="TV(AAC)M1"
gene complement(346826..346931)
/locus_tag="P301_M11466"
tRNA complement(346826..346931)
/locus_tag="P301_M11466"
/product="tRNA-Trp"
/note="TW(CCA)M"
gene 347109..347722
/gene="Far3"
/locus_tag="P301_M11471"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(347592..347955)
/locus_tag="P301_M11476"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(347866..350418)
/gene="Stb2"
/locus_tag="P301_M11481"
mRNA complement(347866..350418)
/gene="Stb2"
/locus_tag="P301_M11481"
/product="Stb2p"
CDS complement(347866..350418)
/gene="Stb2"
/locus_tag="P301_M11481"
/note="Similar to YMR053C Protein that interacts with
Sin3p in a two-hybrid assay and is part of a large protein
complex with Sin3p and Stb1p"
/codon_start=1
/product="Stb2p"
/protein_id="EWG89350.1"
/translation="MVMADTIATGDKTSQFDGKHMQDLERIYSTRDTLDDNFFGERGQ
KDANGDYYDELNNEETNYSGSSYYGHSDDSEVVGLIKNDTVSQLPPLDSFIFPDSRAL
FLLDLGNYADLTYEEIIVHGFEIYIVEQWVACRNLSTLITSYTGNSQDTISGVRVVLP
KDTSMWPGRFRQYFEELMEFARPKFTPKGTLFITNLSGVSFGLNLLHVECGDLRTIWK
DFEVNFDLKNLHCGGRSANLLCPPTMASLDKFSQLFKIPTNGFIAQYPQMIQQQQPRL
PEEEYKTVGNSKCRNTDSKSPVVEMVTLIQISLSYFNLLSKNYQTDGLLCEDTKRAID
EWWETYGKLYLGTEKPRNECTLGPTTVAGLISLILCCYFKLMIENCISSKDPFDEAGF
FQGIYNFQKKHGLNKRKSRVYLDPRTLEKLFEVTAKVSSKDIFKLKKMVTSTVQDIIG
KGNPINLSHKILTTDLDTLIHNIHGGSVGLLWKGKGHPRKCCTDISNEEFLKFNYQRG
DPDGQIREREMLLEKFRLERIAYAQKHASKKVSSSSLDTSEDVGRTNAMPSSATVSSM
FPNYDNTKYAYNFGINKLYQGEYYRRNSFPYCKDRTHDNIYEDLSELKEKSSRLYRCN
SSSAVQNIVEKWDLPFDPSVVRIARDLLRMKYDIQAQQHIQEMDEHYMGKLNKEGTVG
QYSKFNERYKRLQELYKKYSDGAKVFEGRFEDIDNKQQLLLHEMQELNSLSSRLKYDM
RILEVRVRDIESSVAQFDSKLIGLKSSLQGQGKTGICSAIDPKSDKDEYDRCVNDLMT
TNNPTYEALCLKMLSRRYFKDLKNDTVGWFRWLFGNNSLHNNASEDDRGIRV"
gene 350823..353495
/gene="Stv1"
/locus_tag="P301_M11486"
mRNA 350823..353495
/gene="Stv1"
/locus_tag="P301_M11486"
/product="Stv1p"
CDS 350823..353495
/gene="Stv1"
/locus_tag="P301_M11486"
/note="Similar to YMR054W 'Subunit a of the
vacuolar-ATPase V0 domain, one of two isoforms (Stv1p and
Vph1p); Stv1p is located in V-ATPase complexes of the
Golgi and endosomes while Vph1p is located in V-ATPase
complexes of the vacuole'"
/codon_start=1
/product="Stv1p"
/protein_id="EWG89351.1"
/translation="MNQEEAIFRSADMTYVQLYIPLEVIREVTFLLGKMSVFMVMDLN
KDLTAFQRGYVNQLRRFDEVERMVGFLNEVVEKHAAETWKYILHIDDEGNDIAQPDMA
DLINTMEPLSLENVNDMVKEITDCESRARQLDESLDSLRSKLNDLLEQRQVIFECSKF
IEVNPGIAGRATNPEIEQEERDVDEFRMTPDDISETLSDAFSFDDETPQDRGALGNDL
TRNQSVEDLSFLEQGYQHRYMITGSIRRTKVDILNRILWRLLRGNLIFQNFPIEEPLL
EGKEKVEKDCFIIFTHGETLLKKVKRVIDSLNGKIVSLNTRSSELVDTLNRQIDDLQR
ILDTTEQTLHTELLVIHDQLPVWSAMTKREKYVYTTLNKFQQESQGLIAEGWVPSTEL
IHLQDSLKDYIETLGSEYSTVFNVILTNKLPPTYHRTNKFTQAFQSIVDAYGIATYKE
INAGLATVVTFPFMFAIMFGDMGHGFILFLMALFLVLNERKFGAMHRDEIFDMAFTGR
YVLLLMGAFSVYTGLLYNDIFSKSMTIFKSGWQWPSTFRKGESIEAKKTGVYPFGLDF
AWHGTDNGLLFSNSYKMKLSILMGYAHMTYSFMFSYINYRAKNSKVDIIGNFIPGLVF
MQSIFGYLSWAIVYKWSKDWIKDDKPAPGLLNMLINMFLAPGTIDDQLYSGQAKLQVV
LLLAALVCVPWLLLYKPLTLRRLNKNGGGGRPHGYQSVGNIEHEEQIAQQRHSAEGFQ
GMIISDVASVADSINESVGGGEQGPFNFGDVMIHQVIHTIEFCLNCISHTASYLRLWA
LSLAHAQLSSVLWDMTISNAFSSKNSGSPLAVMKVVILFAMWFVLTVCILVFMEGTSA
MLHALRLHWVEAMSKFFEGEGYAYEPFSFRAIIE"
gene complement(353620..354540)
/gene="Bub2"
/locus_tag="P301_M11491"
mRNA complement(353620..354540)
/gene="Bub2"
/locus_tag="P301_M11491"
/product="Bub2p"
CDS complement(353620..354540)
/gene="Bub2"
/locus_tag="P301_M11491"
/note="Similar to YMR055C Mitotic exit network regulator,
forms GTPase-activating Bfa1p-Bub2p complex that binds
Tem1p and spindle pole bodies, blocks cell cycle
progression before anaphase in response to spindle and
kinetochore damage"
/codon_start=1
/product="Bub2p"
/protein_id="EWG89352.1"
/translation="MTSIEDLISNPPLLLHSSLSQLRYLILSEGLPISEDKQQQRTRC
YVWTVLSQTSMEASTQRYLALLKLGPPSTTIYQKIKNDTSRTFQTDPNFRNRVSEDAL
IRCLSCFAWQTQQRRQKTRFGRIPVSTYVQGMNVLLAPLLYSCPSEPMAYQLFTKLCY
EMIPTYLTKNLNGAQNGAKLLDISLRIIDPKLSKFLSDNLLTAEIYGMPSILTLSSCN
KPLDQVIKLWDFMFAYGFHMNILFVVAFLVKMRSKVFKSDSPVNLLRQFPDFDADEII
RLGVGFIAKIPAQIYDLLVDHLTDPDIYIP"
gene complement(354840..355769)
/gene="Aac1"
/locus_tag="P301_M11496"
mRNA complement(354840..355769)
/gene="Aac1"
/locus_tag="P301_M11496"
/product="Aac1p"
CDS complement(354840..355769)
/gene="Aac1"
/locus_tag="P301_M11496"
/note="Similar to YMR056C 'Mitochondrial inner membrane
ADP/ATP translocator, exchanges cytosolic ADP for
mitochondrially synthesized ATP; phosphorylated; Aac1p is
a minor isoform while Pet9p is the major ADP/ATP
translocator'"
/codon_start=1
/product="Aac1p"
/protein_id="EWG89353.1"
/translation="MSHTETQTQQSHFGMDFLMGGVSAAIAKTGAAPIERVKLLMQNQ
EEMLKQGSLDTRYKGILDCFKRTATHEGIVSFWRGNTANVLRYFPTQALNFAFKDKIK
SLLSYDRERDGYAKWFAGNLFSGGAAGGLSLLFVYSLDYARTRLAADARGSKSTSQRQ
FNGLLDVYKKTLKTDGLLGLYRGFVPSVLGIIVYRGLYFGLYDSFKPVLLTGALEGSF
VASFLLGWVITMGASTASYPLDTVRRRMMMTSGQTIKYDGALDCLRKIVQKEGAYSLF
KGCGANIFRGVAAAGVISLYDQLQLIMFGKKFK"
gene 356333..358243
/gene="Fet3"
/locus_tag="P301_M11511"
mRNA 356333..358243
/gene="Fet3"
/locus_tag="P301_M11511"
/product="Fet3p"
CDS 356333..358243
/gene="Fet3"
/locus_tag="P301_M11511"
/note="Similar to YMR058W Ferro-O2-oxidoreductase required
for high-affinity iron uptake and involved in mediating
resistance to copper ion toxicity, belongs to class of
integral membrane multicopper oxidases"
/codon_start=1
/product="Fet3p"
/protein_id="EWG89354.1"
/translation="MTNTLLSIAVLLFSMLSLAQAETHTFNWTTGWDYRNVDGLKSRP
VITCNGQFPWPDITVNKGDRVQVYLTNGMNNTNTSMHFHGLFQNGTASMDGVPFLTQC
PIAPGSTMLYNFTVDYNVGTYWYHSHTDGQYEDGMKGLFIIKDDSFPYDYDEELSLSL
SEWYHDLVTDLTKSFMSVYNPTGAEPIPQNLIVNNTMNLTWEVQPDTTYLLRIVNVGG
FVSQYFWIEDHEMTVVEIDGITTEKNVTDMLYITVAQRYTVLVHTKNDTDKNFAIMQK
FDDTMLDVIPSDLQLNATSYMVYNKTAALPTQNYVDSIDNFLDDFYLQPYEKEAIYGE
PDHVITVDVVMDNLKNGVNYAFFNNITYTAPKVPTLMTVLSSGDQANNSEIYGSNTHT
FILEKDEIVEIVLNNQDTGTHPFHLHGHAFQTIQRDRTYDDALGEVPHSFDPDNHPAF
PEYPMRRDTLYVRPQSNFVIRFKADNPGVWFFHCHIEWHLLQGLGLVLVEDPFGIQDA
HSQQISENHLEVCQSCSVATEGNAAANTLDLTDLTGENVQHAFIPTGFTKEGIIAMTF
SCFAGILGIITIAIYGMMDMEDATEKVIRDLHVDPEVLLNEVDENEEREVNEDRHSTE
KHQFLTKAKRFF"
assembly_gap 358373..358522
/estimated_length=150
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 358700..359086
/gene="Sen15"
/locus_tag="P301_M11516"
mRNA 358700..359086
/gene="Sen15"
/locus_tag="P301_M11516"
/product="Sen15p"
CDS 358700..359086
/gene="Sen15"
/locus_tag="P301_M11516"
/note="Similar to YMR059W Subunit of the tRNA splicing
endonuclease, which is composed of Sen2p, Sen15p, Sen34p,
and Sen54p"
/codon_start=1
/product="Sen15p"
/protein_id="EWG89355.1"
/translation="MATTDIISLVKNNLLYFQMWTEVEILQDDLSWKGNSLRLLRGRP
PHKLSNDVDTEHENSLSSPRPLEFILPINMSQYKENFLTLECLSQTFTHLCSPSTERI
LLAIINDDGTIVYYFVYKGVRKPKRN"
gene complement(359133..360116)
/gene="Sam37"
/locus_tag="P301_M11521"
mRNA complement(359133..360116)
/gene="Sam37"
/locus_tag="P301_M11521"
/product="Sam37p"
CDS complement(359133..360116)
/gene="Sam37"
/locus_tag="P301_M11521"
/note="Similar to YMR060C 'Component of the Sorting and
Assembly Machinery (SAM or TOB complex) of the
mitochondrial outer membrane, which binds precursors of
beta-barrel proteins and facilitates their outer membrane
insertion; contributes to SAM complex stability'"
/codon_start=1
/product="Sam37p"
/protein_id="EWG89356.1"
/translation="MVKGSVHLWGKDGEASLISVDSIALVWFIKLCTSEEAKSMVAGL
QIVFSNNTDLSSDGKLPVLILDNGTKVSGYVNIVQFLHKNICTSKYEKGTDYEEDLAI
VGKKDRLLEYSLLNYVDVEISRLTDYQLFLNTKNYNEYTKKLFSKLLYFPMWYNTPLQ
LRSQARENCEEIIGSLTLEDDEEFVESKAMGSASQLAQSKTFKIAHKNKIKGKQELQQ
VKYNLQFDNRLQSCVSNWLAARKKLDDSVILSSDLLFLANLYVQLGLPDGNRIRSKLE
QTFGSELLNSMSNKIDDFVHRPSNNLEQRDPQFREQGNVVMSLYNLACKYI"
gene 360355..362388
/gene="Rna14"
/locus_tag="P301_M11526"
mRNA 360355..362388
/gene="Rna14"
/locus_tag="P301_M11526"
/product="Rna14p"
CDS 360355..362388
/gene="Rna14"
/locus_tag="P301_M11526"
/note="Similar to YMR061W 'Cleavage and polyadenylation
factor I (CF I) component involved in cleavage and
polyadenylation of mRNA 3' ends; bridges interaction
between Rna15p and Hrp1p in the CF I complex'"
/codon_start=1
/product="Rna14p"
/protein_id="EWG89357.1"
/translation="MSSSTTPDLLYPSADKVAEPSDNIHGDELRLRERIKDNPTNILS
YFQLIQYLETQESYAKVREVYEQFHNTFPFYSPAWTLQLKGELARDEFETVEKILAQC
LSGKLENNDLSLWSTYLDYIRRKNNLITGGQEARAVIVKAFQLVMQKCAIFEPKSSSF
WNEYLNFLEQWKPFNKWEEQQRIDMLREFYKKMLCVPFDNLEKMWNRYTQWEQEINSL
TARKFIGELSAEYMKARSLYQEWLNVTNGLKRASPINLRTANKKNIPQPGTSDSNIQQ
LQIWLNWIKWERENKLMLSEDMLSQRISYVYKQGIQYMIFSAEMWYDYSMYISENSDR
QNILYTALLANPDSPSLTFKLSECYELDNDSESVSNCFDKCTQTLLSQYKKIASDVNS
GEDNNTEYEQELLYKQREKLTFVFCVYMNTMKRISGLSAARTVFGKCRKLKRILTHDV
YVENAYLEFQNQNDYKTAFKVLELGLKYFQNDGVYINKYLDFLIFLNKDSQIKTLFET
SVEKVQDLTQLKEIYKKMISYESKFGNLNNVYSLEKRFFERFPQENLIEVFTNRYQIQ
NSNLIKKLELTYMYNEEEDSYFSSGNGDGHHGSYNMSSSDRKRLMEETGNNGNFSNKK
FKRDSELPTEVLDLLSVIPKRQYFNTNLLDAQKLVNFLNDQVEMPTVESTKSG"
gene complement(362652..363977)
/gene="Ecm40"
/locus_tag="P301_M11531"
mRNA complement(362652..363977)
/gene="Ecm40"
/locus_tag="P301_M11531"
/product="Ecm40p"
CDS complement(362652..363977)
/gene="Ecm40"
/locus_tag="P301_M11531"
/note="Similar to YMR062C 'Mitochondrial ornithine
acetyltransferase, catalyzes the fifth step in arginine
biosynthesis; also possesses acetylglutamate synthase
activity, regenerates acetylglutamate while forming
ornithine'"
/codon_start=1
/product="Ecm40p"
/protein_id="EWG89358.1"
/translation="MRISSTLLQRSKQLIDKYALYVPKTGSFPKGFEVGYTASGVKKN
GSLDLGVILNTNKSRPSTAAAVFTTNKFKAAPVLTSKKVLETARGKNINAIVVNSGCA
NSVTGDLGMKDAQVMIDLVNDKIGQKNSTLVMSTGVIGQRLQMDKISTGINKIFGEEK
FGSDFNSWLNVAKSICTTDTFPKLVTSRFKLPSGTEYTLTGMAKGAGMICPNMATLLG
FIVTDLPIESKALQKMLTFATTRSFNCISVDGDMSTNDTICMLANGAIDTKEINEDSK
DFEQVKLQVTEFAQRLAQLVVRDGEGSTKFVTVNVKNALHFEDAKIIAESISNSMLVK
TALYGQDANWGRILCAIGYAKLNDLKSLDVNKINVSFIATDNSEPRELKLVANGVPQL
EIDETRASEILALNDLEVSVDLGTGDQAAQFWTCDLSHEYVTINGDYRS"
gene 364676..365395
/gene="Rim9"
/locus_tag="P301_M11541"
mRNA 364676..365395
/gene="Rim9"
/locus_tag="P301_M11541"
/product="Rim9p"
CDS 364676..365395
/gene="Rim9"
/locus_tag="P301_M11541"
/note="Similar to YMR063W 'Protein of unknown function,
involved in the proteolytic activation of Rim101p in
response to alkaline pH; has similarity to A. nidulans
PalI; putative membrane protein'"
/codon_start=1
/product="Rim9p"
/protein_id="EWG89359.1"
/translation="MVSMIHIVVFLLAITTIFEILPLITVPVTKYLSLSSFRNHYYGL
FGWCVRGQNQELMCTKMKIGYDSTDVDSSGHVLTLPSNSKVVVSNLLVVHPISLAFTG
TLLILAVIIMVTPLGDSPEMLLFTALFSLPTFMLCLLCFLVDILLFISKLDWPGWLML
AATISVALCCSMLWVMRRVVSVKKYESQQSIAHACSMEQYSISDIYQSKQNGNSSEYE
VAPTHTDSLIAPEVTYRGFIE"
gene 365502..367058
/gene="Aep1"
/locus_tag="P301_M11546"
mRNA 365502..367058
/gene="Aep1"
/locus_tag="P301_M11546"
/product="Aep1p"
CDS 365502..367058
/gene="Aep1"
/locus_tag="P301_M11546"
/note="Similar to YMR064W Protein required for expression
of the mitochondrial OLI1 gene encoding subunit 9 of F1-F0
ATP synthase"
/codon_start=1
/product="Aep1p"
/protein_id="EWG89360.1"
/translation="MITTVQEISKWRNLCFIRMQSRKWYPVLKKTPLVADGRKIIKHA
DKVPHPGEIIHPFYQPTAIEQFTACATEYNPSLLDGKKIAPSLIKHPVSLKTILVDSK
LKFDDIRGVNRWLMEFVARRQHQRNIVLTPASKSVRSFHVLHLSSTDIAKLRGLENVL
SEIENTNDLQSRVESVNNELQNIFDRDSKQTRLFCEDILAYLIKNYGNSTENLILLIN
VTEMQLFSRLDQMKAMNIILYHILCKVEANENPPYSPTLVTALENLLAAINNRFFPGR
CENSLHPIVIEQLLSYFIKTGNLNESKNFLGHLIKKGILPEATIINRYLEAIDVHFDK
STKIFDIRSKFAFIADLAPIIENYGTIDLFKFLIPMCRHFDELCSLLNIIRKSNNAKQ
AVDSTLPIFIKKVLTFTKDPMINSGNLSTVFNIVSPIYGQNVPSEFVEKFILSFALQG
NYTMMAHMIDTYKIKLSHKYQLQIIRALKNSERNHALKNTGAVGYNKEFKKYFIEKYL
NCTEREALRP"
gene 367300..368815
/gene="Kar5"
/locus_tag="P301_M11551"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 369140..371887
/gene="Sov1"
/locus_tag="P301_M11556"
mRNA 369140..371887
/gene="Sov1"
/locus_tag="P301_M11556"
/product="Sov1p"
CDS 369140..371887
/gene="Sov1"
/locus_tag="P301_M11556"
/note="Similar to YMR066W Mitochondrial protein of unknown
function"
/codon_start=1
/product="Sov1p"
/protein_id="EWG89361.1"
/translation="MFKYNRSLCSSALIAKSQIRFYRLKRAPLNYASHIPEVLNKTII
GPDEPEKCLILKGKTSEEIENNLLSNKKFQEINPLDTIQETFIQYLKFCNETNFKRSN
KNLNRLKKTLESKDSNSTVKINAVFNYLLEECDLEIKRLNTTGQTQVYNEEKGNEDDL
EQSIMNDIFRSAQEQFEDQEGHIPLRSTSFLLEILKSFNERFNGIIKPKESITEMVTF
SQLAQAFEVVKLIPVQEMKEKGIYLVGNLLYGTGKVRLDPINESFYIESLLVFGNYKA
AYSLFITNKDKVNERWWNELGLMITLRSNHLRNFRKLLAETDAKYSTKYSYLSPRIAK
LSIRKYLSIGNVTEANILTDRFIKLVEEVGIIRMKDEQEELPTGVKNFQNEKHATEFL
NELEIPSDHDYISIVDFHLYKRNIPMAAQLISKYMEIPGTTQEDAAFLLVKTKLNMLK
DFENLRNIFAQNKDYVVPENNVKMLQEAFESVITKYNTNSPIYNELLFENVSALTKSI
VLTDFLEEFITKQASGQWMELNSVSRSRKFNGLLNILLGTGEEEKAYNILKKLEEASK
KSKTDPGLLYNQFYSEVNAYHYAKFVEFYSLQIQNMKAQNTPSFRKKEFKQKVKSLLK
RMQESEVIPNAVFLREILNFYDSMYDFNSSFEIINPLLESKQQVSSESSLSTSNPCRF
YNRRIITKPLYHKIWSVYCHYYHVLQNNSRILSKKSSIVKKLIKRQIKIHPTCHPRVL
FQMTTENGEILPDKTFSKLIVSTFMKSGDLEAIPAILTFLTKKFDLNIDYDLSMYILK
GLKRQYLRDISNISKDACEYKLRKAELMNXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCTY
L"
assembly_gap 371621..371872
/estimated_length=252
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(371922..373172)
/gene="Ubx4"
/locus_tag="P301_M11561"
mRNA complement(371922..373172)
/gene="Ubx4"
/locus_tag="P301_M11561"
/product="Ubx4p"
CDS complement(371922..373172)
/gene="Ubx4"
/locus_tag="P301_M11561"
/note="Similar to YMR067C UBX (ubiquitin regulatory X)
domain-containing protein that interacts with Cdc48p"
/codon_start=1
/product="Ubx4p"
/protein_id="EWG89362.1"
/translation="MPMVTVKYNFQLFKCKVSLNSTLNDVLHQSIQFFQLHTSSNDWS
LIHLDKPVPLDLPWRLLNLPTGVNLELSKSSNFPVANKTNREDIPFNMIKIRFQIPGR
DSVVKEMLSDQPIAPILRQMSGAAGDDFKIQVFSKIIEFKTIKDENLTLENLGIQEPS
SVRLIFNNTSHSEGISANSAIHPKQTPPTMTNPETVASLPPHELHKPSVFLPSDEPLA
VIKDQIEDEEDYELTVEQAKKYQKMLSSKAGTLGGPILTKRLREQSANNLLKKNKAIS
ECLLRVKFPDRSHIQIAFKPNEDMRTVYNVVSQFLIDENMPFTLNQSHPFKPLAKDDK
KLLDDLEFGSKTMLLFETNSNSNGPLIKAHLLEDAQKITHETRTTPSVNTINKSNPQG
PSDNATSIKKTLNRVPKWMKLSKK"
gene 373899..375179
/gene="Avo2"
/locus_tag="P301_M11566"
mRNA 373899..375179
/gene="Avo2"
/locus_tag="P301_M11566"
/product="Avo2p"
CDS 373899..375179
/gene="Avo2"
/locus_tag="P301_M11566"
/note="Similar to YMR068W Component of a complex
containing the Tor2p kinase and other proteins, which may
have a role in regulation of cell growth"
/codon_start=1
/product="Avo2p"
/protein_id="EWG89363.1"
/translation="MLKEPSVRLREAIIEGNLLIVKRLLRRNPDLLTNIDSENGWSSL
HYASYHGRYLICVYLIQLGHDKHELIKTFKGNTCVHLALMKGHEQTLHLLLQQFPRFI
NHRGENGRAPIHIACMNDYYQCLSLLIGVGADLWVMDTNGDTPLHVCLEYGSISCMKM
LLNEGEVSLDDNVRDKGNWKPIDVAQTFEVGNIYSKVLKEVKKKGPPLGAGKKPSSFR
TPILNAKATFEDGPSPVLSMNSPYSLYSNNSPLPVLPRRISTHTTSGNGGNRRSSITN
PVFNPRKPTLSTDSFSSSSNSSSRLRVNSINVKTPVGVSPKKELVSESVRHSATPTSP
HNNIALINRYLLPNKSNDNVRGDSQTATINDDGGGGNGGDATIGMGLRKDPDDENENK
YKIKVNNGEPRRRVSLLNIPISKLRNSNNTRAED"
gene 375303..376160
/gene="Nat4"
/locus_tag="P301_M11571"
mRNA 375303..376160
/gene="Nat4"
/locus_tag="P301_M11571"
/product="Nat4p"
CDS 375303..376160
/gene="Nat4"
/locus_tag="P301_M11571"
/note="Similar to YMR069W N alpha-acetyl-transferase,
involved in acetylation of the N-terminal residues of
histones H4 and H2A"
/codon_start=1
/product="Nat4p"
/protein_id="EWG89364.1"
/translation="MRSSVYSENTYNCIRTSKEHLTERRRVAMAPMFQHFLNLCVEKF
PESIEHKDTDGNGNFTTAILEREIIYIPEDDTDSIDSVDSLKCINYKLHKSRGDQVLD
ACVQLIDKHLGAKYRRASRIMYGNRKPWKANKLAEMKSAGLVYVCYWDNGVLGAFTSF
MLTEETGLVEGDALHEVSVPVIYLYEAHVASAHRGHGIGRRLLEHALCDGVARHTRRM
CDNFFGVALTVFSDNTRARRLYEALGFYRAPGSPAPASPTIRHTRHGGGRVVVPCDPL
YYVYCLHMP"
gene 376748..378238
/gene="Mot3"
/locus_tag="P301_M11586"
mRNA 376748..378238
/gene="Mot3"
/locus_tag="P301_M11586"
/product="Mot3p"
CDS 376748..378238
/gene="Mot3"
/locus_tag="P301_M11586"
/note="Similar to YMR070W 'Nuclear transcription factor
with two Cys2-His2 zinc fingers; involved in repression of
a subset of hypoxic genes by Rox1p, repression of several
DAN/TIR genes during aerobic growth, and repression of
ergosterol biosynthetic genes'"
/codon_start=1
/product="Mot3p"
/protein_id="EWG89365.1"
/translation="MNADHHLQQQQQQRQQHQQQQHQHQHQHQQHQHQHQHQQQQHTI
LQNVSNTNNIGSDSLASQPFNTTTVSSNKDDVMVNSGARELPMPLHQQQYIYPYYQYT
SNNSNNNNVTAGNNMSASPIVHNNSNNSNNSNISASDYTVANNSTSNNNNNNNNNNNN
NNNIHPNQFAAAANMNSNAAAAAYYSFPTANMPIPQQDQQYMFNPASYISHYYSAVNS
NNNGNNAANNGSNNSSHSAPAPAPGPPHHHHHHSNTHNNLNNGGAVNTNNAPQHHPTI
ITDQFQFQLQQNPSPNLNLNINPAQPLHLPPGWKINTMPQPRPTTAPNHPPAPVPSSN
PVASNLVPAPSSDHKYIHQCQFCEKSFKRKSWLKRHLLSHSQQRHFLCPWCLSRQKRK
DNLLQHMKLKHTNYLLDELKKNNIIFNYNNSSSSNNNNDNNNNNNSNSASGSGGAGAA
AAAATAPENEDGNGYDTNIKTLINDGVLNKDDVKRVLNNLIVSHNK"
gene complement(378374..378877)
/gene="Tvp18"
/locus_tag="P301_M11591"
mRNA complement(378374..378877)
/gene="Tvp18"
/locus_tag="P301_M11591"
/product="Tvp18p"
CDS complement(378374..378877)
/gene="Tvp18"
/locus_tag="P301_M11591"
/note="Similar to YMR071C 'Integral membrane protein
localized to late Golgi vesicles along with the v-SNARE
Tlg2p; may interact with ribosomes, based on
co-purification experiments'"
/codon_start=1
/product="Tvp18p"
/protein_id="EWG89366.1"
/translation="MALSLGQFINVGGMVKDLKSFNFSVYGRWFGYINIILCIALGIA
NLFHVSGVIAFGIISIIQGLVILFIEIPFLLKICPLSDNFIEFIKRFETNGWRCLFYL
AMAIIQYISIAVMATSLIVVAVGLTISSISYAVAYTKHQEFQNTNIIKNPTDDDFPHE
AVVREML"
gene 379619..379732
/gene="Abf2"
/locus_tag="P301_M11596"
mRNA 379619..379732
/gene="Abf2"
/locus_tag="P301_M11596"
/product="Abf2p"
CDS 379619..379732
/gene="Abf2"
/locus_tag="P301_M11596"
/note="Similar to YMR072W 'Mitochondrial DNA-binding
protein involved in mitochondrial DNA replication and
recombination, member of HMG1 DNA-binding protein family;
activity may be regulated by protein kinase A
phosphorylation'"
/codon_start=1
/product="Abf2p"
/protein_id="EWG89367.1"
/translation="MKIIGDKWQSLDQSIKDKYIQEYKKAIQEYNARYPLN"
gene complement(379832..380485)
/gene="Irc21"
/locus_tag="P301_M11601"
mRNA complement(379832..380485)
/gene="Irc21"
/locus_tag="P301_M11601"
/product="Irc21p"
CDS complement(379832..380485)
/gene="Irc21"
/locus_tag="P301_M11601"
/note="Similar to YMR073C 'Putative protein of unknown
function; may be involved in resistance to carboplatin and
cisplatin; null mutant displays increase in spontaneous
Rad52p foci; contains a lipid-binding domain and binds
cardiolipin in a large-scale study'"
/codon_start=1
/product="Irc21p"
/protein_id="EWG89368.1"
/translation="MSSDGMNRDVSNSKPNVRFAAPQRLSVAHPTISSPLHMPMSKSS
RKPLVRTKIRLDPGHSALDWHSLTSNPANYYTKFVSLQLIQDLLDDPVFQKDNFKFSP
SQLKNQLLVQKIPLYKIMPPLRINRKIVKKHCKGEDELWCVINGKVYDISSYLKFHPG
GTDILIKHRNSDDLITYFNKYHQWVNYEKLLQVCLHWRCMRIKVTRDKKKFEDLHRI"
gene complement(380514..381086)
/locus_tag="P301_M11606"
mRNA complement(380514..381086)
/locus_tag="P301_M11606"
/product="hypothetical protein"
CDS complement(380514..381086)
/locus_tag="P301_M11606"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89369.1"
/translation="MDPELQAIREARLAQLKNNSGGTNGDRNSGANNGGGENSAPVGA
AIANFLEPQALERLSRVALVRRDRAQAVETYLKKLIATNNVTHKITEAEIVSILNGIA
KQQNSQNNSKIIFERKDFSEDLNSFDKQNAKNDDXXXXXXXXXXXXXXXXXXXXXXXT
FSTNHILYRTRVKVTLIMCSKRTNKQNITY"
assembly_gap 380610..380676
/estimated_length=67
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 381595..383650
/gene="Rco1"
/locus_tag="P301_M11611"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 383240..383315
/estimated_length=76
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(383808..387641)
/gene="Pds5"
/locus_tag="P301_M11621"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(388200..388273)
/locus_tag="P301_M11631"
tRNA complement(388200..388273)
/locus_tag="P301_M11631"
/product="tRNA-Val"
/note="TV(AAC)M2"
gene complement(389093..389757)
/gene="Vps20"
/locus_tag="P301_M11641"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(390111..392336)
/gene="Ctf18"
/locus_tag="P301_M11651"
mRNA complement(390111..392336)
/gene="Ctf18"
/locus_tag="P301_M11651"
/product="Ctf18p"
CDS complement(390111..392336)
/gene="Ctf18"
/locus_tag="P301_M11651"
/note="Similar to YMR078C 'Subunit of a complex with Ctf8p
that shares some subunits with Replication Factor C and is
required for sister chromatid cohesion; may have
overlapping functions with Rad24p in the DNA damage
replication checkpoint'"
/codon_start=1
/product="Ctf18p"
/protein_id="EWG89370.1"
/translation="MVDTAPYIGSLGRSSLFDTGDIEQAPGNNAIGINEEDIHAFVSS
TGETVQLKKKPAKLATGNISLYTNPDTVWRSDDTYGININYLLDKIEASGDDRTNAQK
TSPITGKIGSDTLWVEKWRPKKFLDLVGNEKTNRRMLGWLRQWTPAVFKEQLPKLPTE
KEVSDMELDPLKRPPKKILLLHGPPGIGKTSVAHVIAKQSGFSVSEINASDERAGPMV
KEKIYNLLFNHTFDTNPVCLVADEIDGSIESGFIRILVDIMQSDIKATNKLLYGQPDK
KDKKRKKKRSKLLTRPIICICNNLYAPSLEKLKPFCEIIAVKRPSDTTLLERLNLICH
KENMNIPIKAINDLIDLAQGDVRNCINNLQFLASNVDSRDSSASDKPACAKNTWASSN
KDSPISWFKIVNQLFRKDPHRDIKEQFYELLNQVELNGNSDRILQGCFNIFPYVKYSD
NGIRKPANISDWLFFHDLMYQSMYAHNGELLRYSALVPLVFFQTFGDIANKDDIRMKN
SEYEQRELKRANSDIVSLIMRHISVQSPLMASFTDRKSLIFEILPYLDSMISSDFNKI
RNLKLKQAIMEELVQLLKSFQLNLIQNRSEGFDVRGGLTIDPPIDEVVLLNPKHINEV
QHKRANNLSSLLAKIEENRAKKRHIDQVTEDRLQSQEMHSKKVKTGLNSSSSTIDFFK
NQYGLLKQTQELEETQKTIGSDETNQADDCNQTVKIWVKYNEGFSNAVRKNVTWNNLW
E"
gene 392597..393667
/gene="Sec14"
/locus_tag="P301_M11656"
mRNA join(392597..392605,392762..393667)
/gene="Sec14"
/locus_tag="P301_M11656"
/product="Sec14p"
CDS join(392597..392605,392762..393667)
/gene="Sec14"
/locus_tag="P301_M11656"
/note="Similar to YMR079W
'Phosphatidylinositol/phosphatidylcholine transfer
protein; involved in regulating PtdIns, PtdCho, and
ceramide metabolism, products of which regulate
intracellular transport and UPR; functionally homologous
to mammalian PITPs'"
/codon_start=1
/product="Sec14p"
/protein_id="EWG89371.1"
/translation="MVTQQEKEFLESYPQNCPPDALPGTPGNLDSAQEKALAELRKLL
EDAGFIERLDDSTLLRFLRARKFDVQLAKEMFENCEKWRKDYGTDTILQDFHYDEKPL
IAKFYPQYYHKTDKDGRPVYFEELGAVNLHEMNKVTSEERMLKNLVWEYESVVQYRLP
ACSRAAGHLVETSCTIMDLKGISISSAYSVMSYVREASYISQNYYPERMGKFYIINAP
FGFSTAFRLFKPFLDPVTVSKIFILGSSYQKELLKQIPAENLPVKFGGKSEVDESKGG
LYLSDIGPWRDPKYIGPEGEAPEAFSMK"
gene complement(394319..397234)
/gene="Nam7"
/locus_tag="P301_M11661"
mRNA complement(394319..397234)
/gene="Nam7"
/locus_tag="P301_M11661"
/product="Nam7p"
CDS complement(394319..397234)
/gene="Nam7"
/locus_tag="P301_M11661"
/note="Similar to YMR080C 'ATP-dependent RNA helicase of
the SFI superfamily involved in nonsense mediated mRNA
decay; required for efficient translation termination at
nonsense codons and targeting of NMD substrates to
P-bodies; involved in telomere maintenance'"
/codon_start=1
/product="Nam7p"
/protein_id="EWG89372.1"
/translation="MVGSGSHTPYDISNSPSDVNVQPATQLNSTLVEDDDVDNQLFEE
AQVTETGFRSASASDNSCAYCGIDSAKCVIKCNSCKKWFCNTKNGTSSSHIVNHLVLS
HHNVVSLHPDSDLGDTVLECYNCGRKNVFLLGFVSAKSEAVVVLLCRIPCAQTKNANW
DTDQWQPLIEDRQLLSWVAEQPTEEEKLKARLITPSQISKLEAKWRSNKDATINDIDA
PEEQEAIPPLLLRYQDAYEYQRSYGPLIKLEADYDKQLKESQALEHISVSWSLALNNR
HLASFTLSTFESNELKVAIGDEMILWYSGMQHPDWEGRGYIVRLPNSFQDTFTLELKP
SKTPPPTHLTTGFTAEFIWKGTSYDRMQDALKKFAIDKKSISGYLYYKILGHQVVDIS
FDVPLPKEFSIPNFAQLNSSQSNAVSHVLQRPLSLIQGPPGTGKTVTSATIVYHLSKI
HKDRILVCAPSNVAVDHLAAKLRDLGLKVVRLTAKSREDVESSVSNLALHNLVGRGAK
GELKNLLKLKDEVGELSASDTKRFVKLVRKTEAEILNKADVVCCTCVGAGDKRLDTKF
RTVLIDESTQASEPECLIPIVKGAKQVILVGDHQQLGPVILERKAADAGLKQSLFERL
ISLGHVPIRLEVQYRMNPYLSEFPSNMFYEGSLQNGVTIEQRTVPNSKFPWPIRGIPM
MFWANYGREEISANGTSFLNRIEAMNCERIITKLFRDGVKPEQIGVITPYEGQRAYIL
QYMQMNGSLDKDLYIKVEVASVDAFQGREKDYIILSCVRANEQQAIGFLRDPRRLNVG
LTRAKYGLVILGNPRSLARNTLWNHLLIHFREKGCLVEGTLDNLQLCTVQLVRPQPRK
TERPMNAQFNVESEMGDFPKFQDFDAQSMVSFSGQIGDFGNAFVDNTELSSYINNEYW
NFENFKSAFSQKQNRNEIDDRNLYQEEASHLNSNFARELQREEQKHELSKDFSNLGI"
gene complement(397686..398703)
/gene="Isf1"
/locus_tag="P301_M11671"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 402391..403518
/gene="Adh3"
/locus_tag="P301_M11696"
mRNA 402391..403518
/gene="Adh3"
/locus_tag="P301_M11696"
/product="Adh3p"
CDS 402391..403518
/gene="Adh3"
/locus_tag="P301_M11696"
/note="Similar to YMR083W 'Mitochondrial alcohol
dehydrogenase isozyme III; involved in the shuttling of
mitochondrial NADH to the cytosol under anaerobic
conditions and ethanol production'"
/codon_start=1
/product="Adh3p"
/protein_id="EWG89373.1"
/translation="MLRTSTLFTRRVQPSLFSRNILRLQSTAAIPKTQKGVIFYENKG
KLHYKDIPVPEPKPNEILINVKYSGVCHTDLHAWHGDWPLPVKLPLVGGHEGAGVVVK
LGSNVKGWKVGDLAGIKWLNGSCMTCEFCESGHESNCPDADLSGYTHDGSFQQFATAD
AIQAAKIQQGTDLAEVAPILCAGVTVYKALKEADLKAGDWVAISGAAGGLGSLAVQYA
TAMGYRVLGIDAGEEKEKLFKKLGGEVFIDFTKTKNMVSDIQEATKGGPHGVINVSVS
EAAISLSTEYVRPCGTVVLVGLPANAYVKSEVFSHVVKSINIKGSYVGNRADTREALD
FFSRGLIKSPIKIVGLSELPKVYDLMEKGKILGRYVVDTSK"
gene 404231..406393
/locus_tag="P301_M11701"
mRNA 404231..406393
/locus_tag="P301_M11701"
/product="hypothetical protein"
CDS 404231..406393
/locus_tag="P301_M11701"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89374.1"
/translation="MCGIFGYCNFLIEKTRGEIIDTLIEGLQALEYKEYDSSGISIQG
DELKSLNIYKQTGKISSLKEEIDLYNLNKNLPFISHCGIAHTRRATHGGLRRANCHPH
NSDPSNEFVVVHNGVITNFANLKALLVAKGYVFKSDTDTECIPKLYKHIYDTSIELGY
NLDFHVLTNLVLKELEGSYGLLCTSSHFPDEVVAARKGSPLVIGVKGKTDMDVNFVEV
EYLDQEEDYLKLNTQTKSSGNVLAAAPVKYNTCLRKSPPLRSQYLRNSTTSTFNHGSS
TETPAENGLPRPMEFYLSSDCASLARYVSKVVYLEDNDIAHIYDGELHIHCSKIGSED
FSFRTVQKLELELSKIKKGPYDNFMQKEIYEQCETTKNVMRGRVDAFTNRVVLGGLEN
WLTELRRAKRIIMIASKSSFHSCLAARPIFEELMEVPVNVELALDFVDRNCCIFRNDV
CIFVSRSGETTDTINALNYCIKKEAVTIGVVNCSGSSISRFTHCGVHTNTGPEKGIAT
TKSYTSQYIALVMIALWMSEDLVSKIERRKEIIQALTIIPSQIKEVLELEPLIIELCD
KKLKQHDTFLLLGRGYQFASALEGASKMKEISYVHSESILTDELGHRVLAVASDNPPI
IAFATKDAFSPKIASCIDQIIERKGNPIIICNKGHKIWEQDKQKGNVVTLEVPQTVDC
LQGILNVIPLQLISYWLAIKKDIGVDLPRDSAMSAPDI"
gene 406812..409693
/locus_tag="P301_M11711"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(409630..409967)
/locus_tag="P301_M11716"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 410129..410983
/locus_tag="P301_M11721"
mRNA 410129..410983
/locus_tag="P301_M11721"
/product="hypothetical protein"
CDS 410129..410983
/locus_tag="P301_M11721"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89375.1"
/translation="MTGSLNRHSLLNGVKKMRIILCDTNEVVTNLWQESIPHAYIQND
KYLCIHHGHLQSLMDSMRKGDAIHHGHSYAIVSPGNSYGYLGGGFDKALYNYFGGKPF
ETWFRNQLGGRYHTVGSATVVDLQRCLEEKTIECRDGIRYIIHVPTVVAPSAPIFNPQ
NPLKTGFEPVFNAMWNALMHSPKDIDGLIIPGLCTGYAGVPPIISCKSMAFALRLYMA
GDHISKELKNVLIMYYLQYPFEPFFPESCKTECQKLGIDIEMLKSFNVEKDAIELLIP
RRILTLNL"
gene complement(411016..412704)
/gene="Vba1"
/locus_tag="P301_M11726"
mRNA complement(411016..412704)
/gene="Vba1"
/locus_tag="P301_M11726"
/product="Vba1p"
CDS complement(411016..412704)
/gene="Vba1"
/locus_tag="P301_M11726"
/note="Similar to YMR088C Permease of basic amino acids in
the vacuolar membrane"
/codon_start=1
/product="Vba1p"
/protein_id="EWG89376.1"
/translation="MQTLDETSNLLPPPEEAEAPPLEQKFHEYNLALPKFPILFSLWL
GSFLSSLDSTIVANIMNRVAEEFSESSKKQWIATSFLLTNTAFQPLYGKLSDITGRKS
ALLTAQFFFGLGCLLTCFARNVTEFSIARAICGIGAGGLNAISSIAVSDICTARERGV
YQGYANIVFGFGQLLGAPLGGVFIETIGWRALFGIQVPVIMLCSVLAIKNINIKLFHV
PPMKERYTLKNLSRIDIFGSLSLVATISGVLFLCSSQLNKLYLSLFTIGSFIVFILVE
RYYATEKILPFELLTRSFCLSSAVTVISSFVVFGEIFRSPIYLQLLQNISVTKTGLFL
IFPSISVAVGSLVTGWVLRNTKINLAHCAYQIIFGGMIMQLLGLGLGYLLLSHLNPDY
TIYDMLESITFRSNSIWWKLIYVFASVLVSFGYACLLVATLVSIVFTVEKSQQGTMTG
VFYLWRSIGNVLGASLTLVSYENSLSSMLWNYMFKTKRDDEYHFTKKQYYSLINDSSY
LRGPNFPTDIFVRILDVYKKAFLISYIPNIALAAVGIVLSLYLVKHTYKRSSSS"
gene complement(413211..415689)
/gene="Yta12"
/locus_tag="P301_M11731"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 416849..417532
/locus_tag="P301_M11741"
mRNA 416849..417532
/locus_tag="P301_M11741"
/product="hypothetical protein"
CDS 416849..417532
/locus_tag="P301_M11741"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89377.1"
/translation="MSPMKVAVVGASGKVGRLLINQLKANDSFSTPLAIVRTQDQVNY
FKNEVGVDASLTDIENASVSEITDAIKAYDAVVFSAGAGGKGMERIFTVDLDGCIKVV
EACEKAGIKRFVVVSALKAEDRDFWYNIKGLREYYIAKRSADREVRNSNLDYTILQPG
SLELNKGTGLLQPLDKLEEKASVNYSINREDVASFIVESLLHPNATVKKTISLVNGNE
PMEKFIQSL"
gene complement(417662..418969)
/gene="Npl6"
/locus_tag="P301_M11746"
mRNA complement(417662..418969)
/gene="Npl6"
/locus_tag="P301_M11746"
/product="Npl6p"
CDS complement(417662..418969)
/gene="Npl6"
/locus_tag="P301_M11746"
/note="Similar to YMR091C 'Component of the RSC chromatin
remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p,
and Htl1p to form a module important for a broad range of
RSC functions; involved in nuclear protein import and
maintenance of proper telomere length'"
/codon_start=1
/product="Npl6p"
/protein_id="EWG89378.1"
/translation="MSDSEGGLASEVEHEKRSRSTSNRPNYAIDTEDLDIDENDENED
DDYREEEANEGVNEEEISDEEEQINKSGRNKRRHVDEEEDLSEDKGVTRSRNRSKFKK
PVFPGIDDAEENLNPLKVVNEEYVLPDDPEGETKITADGDLLGGREFLVRTFTLTEKG
NRKFMLATEPARIVGFRDSYLFFQTHPNLYKFILNQTQKNDLIDRGVLPYSYRNRQIA
LVTARGVFKEFGAKIIRGGKHITDDYYASELRTKGNVIEGKLAGDPIDKSARALETMM
YPASENGINPAKNQVEFFEHRPHGHMSNSNIIASGSKLSSTNWLYQHSAACSRFNSDL
FYDRVKVLLVDQQGLRDAYTNILHIPESTQSTTVLGWRRSKNDSPSDTSIVYETVIHD
NDLNKPKTGLSEIPKEIYEDVVDEDVLRAITEQQNFEKCNEYI"
gene complement(419236..421083)
/gene="Aip1"
/locus_tag="P301_M11751"
mRNA complement(419236..421083)
/gene="Aip1"
/locus_tag="P301_M11751"
/product="Aip1p"
CDS complement(419236..421083)
/gene="Aip1"
/locus_tag="P301_M11751"
/note="Similar to YMR092C 'Actin cortical patch component,
interacts with the actin depolymerizing factor cofilin;
required to restrict cofilin localization to cortical
patches; contains WD repeats'"
/codon_start=1
/product="Aip1p"
/protein_id="EWG89379.1"
/translation="MSSISLKEIIPPQPSTQRNFTTHLSYDPTTNAIAYPCGKSAFVR
CLDDGDSKVPPVVQFTGHGSSVVTTVKFSPIKGSQYLCSGDESGKVIVWGWTFDKESN
SVEVNVKSEFQVLAGPISDISWDFEGRRLCVVGEGRDNFGVFISWDSGNSLGEVSGHS
QRINACHLKQSRPMRSMTVGDDGSVVFYQGPPFKFSASDRTHHKQGSFVRDVEFSPDS
GEFVITVGSDRKISCFDGKSGEFLKYIEDDQEPVQGGIFALSWLDSQKFATVGADATI
RVWDITTSKCVQKWTLDKQELGNQQVGVVATGNGRIISLSLDGTLNFYELGHDEVLKT
ISGHNKGITALTVNPLISGSYDGRIMEWSSSSMHQDHSNLIVSLDNSKAQEYSSISWD
DTLKVNGITKHEFGSQPKVASANNDGFTAVLTNDDDLLILQSFTGDIIKSVRLNSPGS
AVSLSQNYVAVGLEEGNTIQVFKLSDLEVSFDLKTPLRAKPSYISISPSETYIAAGDV
MGKILLYDLQSREVKTSRWAFHTSKINAISWKPAEKGANEEEIEEDLVATGSLDTNIF
IYSVKRPMKIIKALNAHKDGVNNLLWETPSTLVSSGADACIKRWNVVLE"
gene 421620..423161
/gene="Utp15"
/locus_tag="P301_M11756"
mRNA 421620..423161
/gene="Utp15"
/locus_tag="P301_M11756"
/product="Utp15p"
CDS 421620..423161
/gene="Utp15"
/locus_tag="P301_M11756"
/note="Similar to YMR093W Nucleolar protein, component of
the small subunit (SSU) processome containing the U3
snoRNA that is involved in processing of pre-18S rRNA"
/codon_start=1
/product="Utp15p"
/protein_id="EWG89380.1"
/translation="MSTARPRIITSKAPLLPQQTTPEQRYWRQYTSAQLVKEHNSVTH
ISFNPQHPHDFAVTSSTRVQIFSSRTRQVIKTFSRFKDVVYSASFRSDGKLLCAGDAT
GLVSVYDSYNPRTILLSINASTHPTHVTKFHTQDNKILATASDDRVTRLWDISNAYEP
QLELTGATDYVRTLSFIPAAPHLVATGSYDGLIRLYDTRSSGSTPIYSLNHDQPVENV
IAVSPTQIVSCGGNNFKVWDLTSNKKLYERGNFNKAVTCLDYVENFDSPMQSALIASS
LDGHVKVFDPLDNFQVKFGWKFSGPVLSCAVSPSTAQGNRHLVAGLSSGLLAIRTKKK
EKRSSDKENAPASFNKNAKSNNFQRMMRGSEYQGDQEHIIHNDKVRSQRRMRAFERNI
NQFKWSEALDNAFVPGMAKELTLTVLQELRKRGKVRVALYGRDESTLEPLLNWCLKGI
EDVRSASIVADWVAVVLELYGNTLESSPVLQELMIDLKTKVRHEIHKSKEAQRIEGML
QLLTS"
gene 423430..424866
/gene="Ctf13"
/locus_tag="P301_M11761"
mRNA 423430..424866
/gene="Ctf13"
/locus_tag="P301_M11761"
/product="Ctf13p"
CDS 423430..424866
/gene="Ctf13"
/locus_tag="P301_M11761"
/note="Similar to YMR094W Subunit of the CBF3 complex,
which binds to the CDE III element of centromeres, bending
the DNA upon binding, and may be involved in sister
chromatid cohesion during mitosis"
/codon_start=1
/product="Ctf13p"
/protein_id="EWG89381.1"
/translation="MPSFNPVRFLELPIDIRKEVYFHLDGNFCGAHPYPIDILYKSND
VELPGKPSYKRSKRSKKLLRYMYPVFATYLNIFEYSPQLIEKWLEYAFWLRYDCLVLD
CFKVNHLYDGTLIDALEWTYLDNELRLAYFNKASMLEVWYTFKEYKKWVIDSVAFDEL
DLLNVSNIQFNIDNLTPQLVDKCLSILEQKDLFATIGEVQFGQDEEVGEEKDVDVSGA
NSDENSSPSSTIKNKKRSASKRSHSDNGNVGATHNQLTSISVIRTIRSMESMKSLRKI
TVRGEKLYELLINFHGFRDNPGKTISYIVKRRINEIRLSRMNQISRTGLADFTRWDNL
QKLVLSRVAYIDLNSIVFPKNFKSLTMKRVSKIKWWNIEENILKELKVDKRTFKSLYI
KEDDSKFTKFFNLRHTRIKELDKSEINQITYLRCQAIVWLSFRTLNHIKLQNVSEVFN
NIIVPRALFDSKRVEIYRCEKISQVLVI"
gene complement(424890..425564)
/gene="Sno1"
/locus_tag="P301_M11766"
mRNA complement(424890..425564)
/gene="Sno1"
/locus_tag="P301_M11766"
/product="Sno1p"
CDS complement(424890..425564)
/gene="Sno1"
/locus_tag="P301_M11766"
/note="Similar to YMR095C 'Protein of unconfirmed
function, involved in pyridoxine metabolism; expression is
induced during stationary phase; forms a putative
glutamine amidotransferase complex with Snz1p, with Sno1p
serving as the glutaminase'"
/codon_start=1
/product="Sno1p"
/protein_id="EWG89382.1"
/translation="MHKTHSTMSGKSMKVIGVLALQGAFLEHTNHLKRCLAENDYGIK
IEIKTVKTPEDLAQCDALIIPGGESTSMSLIAQRTGLYPCLYEFVHNPEKVVWGTCAG
LIFLSAQLENESALVKTLGVLKVDVRRNAFGRQAQSFTQKCDFSNFIPGCDNFPATFI
RAPVIERILDPIAVKSLYELPVNGKDVVVAATQNHNILVTSFHPELADSDTRFHDWFI
RQFVSN"
gene 426013..426906
/gene="Snz1"
/locus_tag="P301_M11771"
mRNA 426013..426906
/gene="Snz1"
/locus_tag="P301_M11771"
/product="Snz1p"
CDS 426013..426906
/gene="Snz1"
/locus_tag="P301_M11771"
/note="Similar to YMR096W 'Protein involved in vitamin B6
biosynthesis; member of a stationary phase-induced gene
family; coregulated with SNO1; interacts with Sno1p and
with Yhr198p, perhaps as a multiprotein complex containing
other Snz and Sno proteins'"
/codon_start=1
/product="Snz1p"
/protein_id="EWG89383.1"
/translation="MTGEDFKIKSGLAQMLKGGVIMDVVTPEQAKIAEKSGACAVMAL
ESIPADMRKSGKVCRMSDPKMIKDIMNSVSIPVMAKVRIGHFVEAQIIEALEVDYIDE
SEVLTPADWTHHIEKDKFKVPFVCGAKDLGEALRRINEGAAMIRTKGEAGTGDVSEAV
KHIRRITEEIKACQQLKSEDDIAKVAEEMRVPVSLLKDVLEKGKLPVVNFAAGGVATP
ADAALLMQLGCDGVFVGSGIFKSSNPVRLATAVVEATTHFDNPSKLLEVSSDLGELMG
GVSIESISHASNGVRLSEIGW"
gene complement(427029..428133)
/gene="Mtg1"
/locus_tag="P301_M11776"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(428377..430215)
/gene="Lrc4"
/locus_tag="P301_M11781"
mRNA complement(428377..430215)
/gene="Lrc4"
/locus_tag="P301_M11781"
/product="Lrc4p"
CDS complement(428377..430215)
/gene="Lrc4"
/locus_tag="P301_M11781"
/note="Similar to YMR098C 'Mitochondrial protein required
for the stability of Oli1p (Atp9p) mRNA and for the Oli1p
ring formation; YMR098C is not an essential gene'"
/codon_start=1
/product="Lrc4p"
/protein_id="EWG89384.1"
/translation="MNKFCLLPFHGKRIGVANIPFTILFKKGPCFLHSHITAVYYSTK
GKNDSHEQSRVSKKSTFTPLETPWYLRIVDNEKELMEGKKNNHHTMNKELEIPKTSPN
SLRKIADLLTGKLGLDDFLVFDLRKKSPNSVSAVNKLGDFMVICTARSTKHCHKSFLE
LNKFLKHEFCSSAYVEGNFNERQESRRKRRLARKSNLSKLLGRSSECSAKDLNSEAWY
MIDCRVDGIFVNILTQRRRNELNLEELYAPENEKSKFQNIDSGNVPTISGVNEISSNN
NILLGLRRLAQQRRRYSTINPNGLSNLRYFLQKEDFKGANKIIQSSSGTETHNIRTLE
HVKNTLKDLVGQERKVDVVQWKSLFDEHSTFLTINQSAAYWPLRLEYAILLNKADPQF
YSDRVFLKDYLLLKKSLGQELIREDLIALLEMVLKTQHSSHSYFNLVKQNRVIIRALN
LFKGLQTEDDGSVVYDEEVISLLLNSMVADERVKLRSLYETIDHIFQTFGDKLTSGMI
VSILQNLAKIKDWNKLLQVWEAITPTEGEGQDKRPWNEFINVINQSGDSHVISKIVNN
GHLLWIRRLNVNVTPELCNSIKALLKTAGMENSTLEEFLVRGTNNQ"
assembly_gap 430541..430837
/estimated_length=297
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 431160..431231
/locus_tag="P301_M11786"
tRNA 431160..431231
/locus_tag="P301_M11786"
/product="tRNA-Asp"
/note="TD(GUC)M"
gene complement(431540..432433)
/locus_tag="P301_M11791"
mRNA complement(431540..432433)
/locus_tag="P301_M11791"
/product="hypothetical protein"
CDS complement(431540..432433)
/locus_tag="P301_M11791"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89385.1"
/translation="MPIKETDKEVVLTHPADETTSVHILKYGATVYSWKLKSEEQLWL
STAAKLDGSKPVRGGIPLVFPVFGKNSTDEHLSKLPQHGLARNSTWEFLGQTKENPPT
VQFGLKPEIANPELTKLWPMDYLLILTVELGSDYLKTAIEVENTSSSKELKFNWLFHT
YFRIEDIEGTMVSNLAGMKLYDQLLKESYVDKHPVVTFNQETDVIYQNVSAERAIQIV
DKGVQIHTLKRYNLPDTVVWNPWIEKSQGMADFEPKTGYQQMICIEPGHVHDFISLAP
GKKWNAYQLLCKEELKYQAIQ"
gene 433906..435768
/gene="Mub1"
/locus_tag="P301_M11801"
mRNA 433906..435768
/gene="Mub1"
/locus_tag="P301_M11801"
/product="Mub1p"
CDS 433906..435768
/gene="Mub1"
/locus_tag="P301_M11801"
/note="Similar to YMR100W 'MYND domain-containing protein
required for ubiquitination and turnover of Rpn4p;
interacts with Ubr2p (E3) and indirectly with Rad6p (E2);
short-lived protein degraded in a Ubr2p/Rad6p dependent
manner; similar to the A. nidulans samB gene'"
/codon_start=1
/product="Mub1p"
/protein_id="EWG89386.1"
/translation="MRDSNHRSLTSNKPIVTITSTVYDRRALDINSSIPLINSLNYLT
YLTSNSSKVRETVANDGALERLVSILRSCHLSLFELLDLDLENFXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSGVLSVLVTVLDNYLLYHKNYDFIKD
QTMTFDFKGITTETMYKFMRKDENETYQQYIEFITGQDKLKLSTDKNFLNERLVAPSM
TIPTDFSDIWGRFADLASNFEPDQERHDDDIDIDSEVESENFDAHKNFFSQPDINRPT
ISTPREFFLGRIVPKQDDVIWSLQLLAFVSKYTYMKSTLQNVELVESLSFRSMAYKIK
QRISEENDLEEQERDVTVKLSSLYPYLSKNPENNSKVKALDTSKMDPFFKELEELSNR
CQQEEQNEICNNHCPVLNLFERYRVPKPSDDNAYGKDKERINLRKKISDNFERRWSYD
KMKKELTNIVYKNKVLTNVVNIFPLVEKYTVSAENTHDVIYWSSVIMRNSCRKNEILG
VRQCANFSCGKWEDFPRQFAKCRRCKRTKYCSRKCQLKAWGYHRYWCHEVGSSHMRST
NTTTGVNTPNEPSSLNATATTAADVSNSTSTFTPNISTTVPDEISNRDENSIPE"
assembly_gap 434170..434305
/estimated_length=136
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 435843..435920
/estimated_length=78
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(436055..437079)
/gene="Srt1"
/locus_tag="P301_M11806"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(437451..439955)
/locus_tag="P301_M11811"
mRNA complement(437451..439955)
/locus_tag="P301_M11811"
/product="hypothetical protein"
CDS complement(437451..439955)
/locus_tag="P301_M11811"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89387.1"
/translation="MTNMAEDKTSISKVHSEFSNSSNTTGFEEESRSHQESFDGESSS
GESKSKSKLNLEYSADIEPLKFRMTKTNNTNDKLKYSNNGNATDSFMRLKEHLQRGNT
LNSNLRVNEFYPFNSIDTEQFENYLREPKYIKMLKRRKNLKQFRRLFLAQELMAYEGE
TVTSTSKSSEPTSKAIWSTKFSRDGKFMATGSKDGKIRIWKVIGSPVERAELDSSAES
NKEARAKSMRIKQQVSSLNNPKEKQFLDSATEKYEEKEKLLNLYAPVFHPTPLRLYKE
HVQDVLDINWSKNNFILSASMDKTVKLWHPDRKNSLKTFIHPDFVTCVEFHPTDDRFF
ISGCLDHKCRLWSILDDEVSFEYDCQDLITSVTLSPEEGKHTIIGTFNGYVHILMTRG
LTPVSSFHVADRQTQEQNAHVMVTETDSKIRHGPRVTGLQAFRSQLDNSFRLVVTSND
SRIRIFDLEQRKLLEVLKGFHSGSSQHKAQLSIWHGQPIVVNSSDDHWVYGWRLKSSD
RENDQDEPKRKPKGLARSGSLRSIFSKSMSRSSSQNNEEKPHHHLKLTNLLPLPHHSN
DHYIKNTDYISFHAHNAPVTCVSIAPPETSKTLSLSNDVICELSLEFFQTSDSFDVLS
RSNDDGIMSDVESSLGYNSKPGSISNASATSAIPDVVDAIGTILISTDNVGTIRVFRA
DMPSVIRKRVLLKLEEYNREVRRRFNSSDSLHSLSRSFNSRAKSNLAGQPAAAYTNTG
KGYATGRGYSNICPKSSTSLKTLGSNAQPRTPRESMSSIFSNAHGPTTPTSAMNLPIR
CNVCSGSRFEAFSGANDQQDRNYYCVDCGTVVNNFR"
gene complement(441021..443053)
/gene="Ypk2"
/locus_tag="P301_M11821"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 443219..443371
/estimated_length=153
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(443409..445118)
/gene="Pgm2"
/locus_tag="P301_M11831"
mRNA complement(443409..445118)
/gene="Pgm2"
/locus_tag="P301_M11831"
/product="Pgm2p"
CDS complement(443409..445118)
/gene="Pgm2"
/locus_tag="P301_M11831"
/note="Similar to YMR105C 'Phosphoglucomutase, catalyzes
the conversion from glucose-1-phosphate to
glucose-6-phosphate, which is a key step in hexose
metabolism; functions as the acceptor for a
Glc-phosphotransferase'"
/codon_start=1
/product="Pgm2p"
/protein_id="EWG89388.1"
/translation="MSFQIETVPTKPYEDQKPGTSGLRKKTKVFKDEPNYTENFIQSI
MEAIPEGSKGATLVVGGDGRYYNDVILHKIAAIGAANGIKKLVIGQHGLLSTPAASHI
MRTYEEKCTGGIILTASHNPGGPENDMGIKYNLSNGGPAPESVTNAIWEISKKLTSYK
IIKDFPELDLGTIGKNKKYGPLLVDIIDITKDYVNFLKEIFDFDLIKKFIDNQRSTKN
WKLLFDSMNGVTGPYGKAIFVDEFGLPADEVLQNWHPSPDFGGMHPDPNLTYASSLVK
RVDREKIEFGAASDGDGDRNMIYGYGPSFVSPGDSVAIIAEYAAEIPYFAKQGIYGLA
RSFPTSGAIDRVAKAHGLNCYEVPTGWKFFCALFDAKKLSICGEESFGTGSNHVREKD
GVWAIMAWLNILAIYNKHHPENEASIKTIQNEFWAKYGRTFFTRYDFEKVETEKANKI
VDQLRAYVTKSGVVNSAFPADESLKVTDCGDFSYTDLDGSVSDHQGLYVKLSNGARFV
LRLSGTGSSGATIRLYIEKYCDDKSQYQKTAEEYLKPIINSVIKFLNFKQVLGTEEPT
VRT"
gene 445575..445769
/locus_tag="P301_M11836"
mRNA 445575..445769
/locus_tag="P301_M11836"
/product="hypothetical protein"
CDS 445575..445769
/locus_tag="P301_M11836"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89389.1"
/translation="MILVHTGNVLYPRFIVVAFTFEQRQGGRCKGGKATCMASVQSYK
VTMQISSMTIIYPLFIFFSL"
gene complement(445812..447701)
/gene="Yku80"
/locus_tag="P301_M11841"
mRNA complement(445812..447701)
/gene="Yku80"
/locus_tag="P301_M11841"
/product="Yku80p"
CDS complement(445812..447701)
/gene="Yku80"
/locus_tag="P301_M11841"
/note="Similar to YMR106C 'Subunit of the telomeric Ku
complex (Yku70p-Yku80p), involved in telomere length
maintenance, structure and telomere position effect;
relocates to sites of double-strand cleavage to promote
nonhomologous end joining during DSB repair'"
/codon_start=1
/product="Yku80p"
/protein_id="EWG89390.1"
/translation="MSSESTTFIVDVSPSMMKNNNVTKSMAYLEYTLLNKSKKSRKTD
WISCYLANCPVSENSQEIPNVFQIQSFLAPVTTTATIGFIKRLKQYCDQHSHDSSNEG
LQSMIQCLLVVSLDIKQQFQARKILKQIVVFTDNLDDLDITDEEIDLLTEELSTRIIL
IDCGKDTQEERKKSNWLKLVEAIPNSRIYNMNELLVEITSPATSVVKPVRVFSGELRL
GADILSTQTSNPSGSMQDENCLCIKVEAFPATKAVSGLNRKTAVEVEDSQKRERYVGV
KSIIEYEIHNEGNKKNVSEDDQSGSSYIPVTISKDSVTKAYRYGADYVVLPSVLVDQT
VYESFPGLDLRGFLNREALPRYFLTSESSFITADTRLGCQSDLMAFSALVDVMLENRK
IAVARYVSKKDSEVNMCALCPVLIEHSNINSEKKFVKSLTLCRLPFAEDERVTDFPKL
LDRTTTSGVPLKKETDGHQIDELMEQFVDSMDTDELPEIPLGNYYQPIGEVTTDTTLP
LPSLNKDQEENKKDPLRIPTVFVYRQQQVLLEWIHQLMINDSREFEIPELPDSLKNKI
SPYTHKKFDSTKLVEVLGIKKVDKLKLDSELKTELEREKIPDLETLLKRGEQHSRGSP
NNSNN"
assembly_gap 448136..448391
/estimated_length=256
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 450435..450675
/estimated_length=241
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 450690..451037
/gene="Spg4"
/locus_tag="P301_M11866"
mRNA 450690..451037
/gene="Spg4"
/locus_tag="P301_M11866"
/product="Spg4p"
CDS 450690..451037
/gene="Spg4"
/locus_tag="P301_M11866"
/note="Similar to YMR107W 'Protein required for survival
at high temperature during stationary phase; not required
for growth on nonfermentable carbon sources'"
/codon_start=1
/product="Spg4p"
/protein_id="EWG89391.1"
/translation="MGSFWDAFAVYDKKKHADPSVYGGNHNNTGDSKTQVMFSKEYRQ
PRTHQQENLQSMRRSSIGSQDSSDVEDVKEGRLPAEVEIPKNVDISNMSQGEFLRLYE
SLRRGEPDNKVNR"
gene 451753..453816
/gene="Ilv2"
/locus_tag="P301_M11871"
mRNA 451753..453816
/gene="Ilv2"
/locus_tag="P301_M11871"
/product="Ilv2p"
CDS 451753..453816
/gene="Ilv2"
/locus_tag="P301_M11871"
/note="Similar to YMR108W 'Acetolactate synthase,
catalyses the first common step in isoleucine and valine
biosynthesis and is the target of several classes of
inhibitors, localizes to the mitochondria; expression of
the gene is under general amino acid control'"
/codon_start=1
/product="Ilv2p"
/protein_id="EWG89392.1"
/translation="MIRQSTLKNFAIKRCFQHIAYRNTPAMRSVALAQRFYSSSSRYY
SASPLPASKRPEPAPSFNVDPLEQPAEPSKLAKKLRAEPDMDTSFVGLTGGQIFNEMM
SRQNVDTVFGYPGGAILPVYDAIHNSDKFNFVLPKHEQGAGHMAEGYARASGKPGVVL
VTSGPGATNVVTPMADAFADGIPMVVFTGQVPTSAIGTDAFQEADVVGISRSCTKWNV
MVKSVEELPLRINEAFEIATSGRPGPVLVDLPKDVTAAILRNPIPTKTTLPSNALNQL
TSRAQDEFVMQSINKAADLINLAKKPVLYVGAGILNHADGPRLLKELSDRAQIPVTTT
LQGLGSFDQEDPKSLDMLGMHGCATANLAVQNADLIIAVGARFDDRVTGNISKFAPEA
RRAAAEGRGGIIHFEVSPKNINKVVQTQIAVEGDATTNLGKMMSKIFPVKERSEWFAQ
INKWKKEYPYAYMEETPGSKIKPQTVIKKLSKVANDTGRHVIVTTGVGQHQMWAAQHW
TWRNPHTFITSGGLGTMGYGLPAAIGAQVAKPESLVIDIDGDASFNMTLTELSSAVQA
GTPVKILILNNEEQGMVTQWQSLFYEHRYSHTHQLNPDFIKLAEAMGLKGLRVKKQEE
LDAKLKEFVSTKGPVLLEVEVDKKVPVLPMVAGGSGLDEFINFDPEVERQQTELRHKR
TGGKH"
gene 454254..457901
/gene="Myo5"
/locus_tag="P301_M11876"
mRNA 454254..457901
/gene="Myo5"
/locus_tag="P301_M11876"
/product="Myo5p"
CDS 454254..457901
/gene="Myo5"
/locus_tag="P301_M11876"
/note="Similar to YMR109W 'One of two type I myosins;
contains proline-rich tail homology 2 (TH2) and SH3
domains; MYO5 deletion has little effect on growth, but
myo3 myo5 double deletion causes severe defects in growth
and actin cytoskeleton organization'"
/codon_start=1
/product="Myo5p"
/protein_id="EWG89393.1"
/translation="MAILKRGARKKVHQEPAKRSANIKKATFDSSKKKEVGVSDLTLL
SKISDEAINENLKKRFLNATIYTYIGHVLISVNPFRDLGIYTDAVMNEYKGKNRLEVP
PHVFAIAESMYYNMKSYNENQCVIISGESGAGKTEAAKRIMQYIAAASSTHTESIGKI
KDMVLATNPLLESFGCAKTLRNNNSSRHGKYLEIKFNNQFEPCAGNITNYLLEKQRVV
SQIKNERNFHIFYQFTKGASDAYRQTFGVQKPEQYVYTAAAGCISAETIDDLQDYQET
LKAMRVIGLGQEEQDQIFRMLAAILWIGNVSFIENEEGNAQVRDTSVTDFVAYLLQID
SQLLIKSLVERIMETNHGMKRGSVYHVPLNIVQADAVRDALAKAIYNNLFDWIVSRVN
KSLQAFPGAEKSIGILDIYGFEIFEHNSFEQICINYVNEKLQQIFIQLTLKSEQETYE
REKIQWTPIKYFDNKVVCDLIEARRPPGIFAAMNDSVATAHADSNAADQAFAQRLNLF
TTNPHFDLRSNKFVIKHYAGDVTYDIDGITDKNKDQLQKDLVELIGTTTNTFLATIFP
DTVDRESKRRPPTAGDKIIKSANDLVETLSKAQPSYIRTIKPNETKSPNDYDDRQVLH
QIKYLGLQENVRIRRAGFAYRQVFEKFVERFYLLSPHCSYAGDYTWQGDTLDAVKYIL
QDSSIPQQEYQLGVTSVFIKTPETLFALEHMRDRYWHNMAARIQRAWRRFLQRRIDAA
TKIQRTIRERKEGNKYEKLRDYGTKVLGGRKERRSMSLLGYRAFMGDYLSCNESKSKG
AYIKRQVSIKEKVIFSIHGEALHTKFGRSAQRLKKTFLLTPTTLYIVGQTLVQNAMTY
TQDYKIDVRNIQAVSLTNLQDDWVAIKLASSGQPDPLINTYFKTELITHLKRLNDKIQ
IKIGSAIEYQKKPGKLHSVKCQINESAPKYGDIYKSSTISVRRGNPPNSQVHKKPRKK
SSISSGYHASSSQATRRPVSIAAAQHVPTAPASRHSKKPAPPPPGMQNKAATRRSVPN
PASTLTASKSNARPSPPTAATRATPAAAAMGSDRQANIPPPPPPPPPSSKPKEPMFEA
AYDFSGSGSPSELPLKKGDVIYITREEPSGWSLGKLLDGSKEGWVPTAYMKPHSGNNN
IPTPPQNRDVPKPVLNSVQHDNTSANVIPAAAQASLGDGLANALAARANKMRLESDDE
EANEDEEEDDW"
gene complement(458049..459647)
/gene="Hfd1"
/locus_tag="P301_M11886"
mRNA complement(458049..459647)
/gene="Hfd1"
/locus_tag="P301_M11886"
/product="Hfd1p"
CDS complement(458049..459647)
/gene="Hfd1"
/locus_tag="P301_M11886"
/note="Similar to YMR110C 'Putative fatty aldehyde
dehydrogenase, located in the mitochondrial outer membrane
and also in lipid particles; has similarity to human fatty
aldehyde dehydrogenase (FALDH) which is implicated in
Sjogren-Larsson syndrome'"
/codon_start=1
/product="Hfd1p"
/protein_id="EWG89394.1"
/translation="MSNDGSKILNYTPVSKIDEIVEISRNFFFEKQLKLSHENNPRKK
DLEFRQLQLKKLYYAVKDHEEELIDAMYKDFHRNKIESVLNETTKLMNDILHLIEILP
KLIKPRRVSDSSPPFMFGKTIVEKISRGSVLIIAPFNFPLLLAFAPLAAALAAGNTIV
LKPSELTPHTAVVMENLLTTAGFPDGLIQVVQGAIDETTRLLDCGKFDLIFYTGSPRV
GSIVAEKAAKSLTPCVLELGGKSPTFITENFKASNIKIALKRIFFGAFGNSGQICVSP
DYLLVHKSIYPKVIKECESVLNEFYPSFDEQTDFTRMIHEPAYKKAVASINSTNGSKI
VPSKISINSDTEDLCLVPPTIVYNIGWDDPLMKQENFAPVLPIIEYEDLDETINKIIE
EHDTPLVQYIFSDSQTEINRILTRLRSGDCVVGDTVIHVGITDAPFGGIGTSGYGNYG
GYYGFNTFSHERTIFKQPYWNDFTLFMRYPPNSAQKEKLVRFAMERKPWFDRNGNNKW
GLRQYFSLSAAVILISTIYAHCSS"
gene complement(460058..461446)
/locus_tag="P301_M11891"
mRNA complement(460058..461446)
/locus_tag="P301_M11891"
/product="hypothetical protein"
CDS complement(460058..461446)
/locus_tag="P301_M11891"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89395.1"
/translation="MPAREYNYVEGFGGYGSLDDDDSDRDSERRNHDLGQRTITTSPT
GVSRHAALNRYMIPGRINPLFRPTDAAQPPIVSTSTSASATEPTNRIGPGRIKETPET
NFNAFLIAQLTRMEEQNANLKEEISLMKKEQELFFLENQKKLEKGFKDINKYVEDVSA
MKEVFKEVVGIMTGERIRFIDHTGENVTPQEAARVGNPSTSTQAHQSQSRSTNWQEYS
MHASILAGDPRIKPEPGLSDFENGEYDGNESDENATTRNLPLNNPDSVSNADDSNNQL
DGTGNENDIRNRRGCVGTSYKLNRAIQNVTDAAREYFEGLPGQPSVLSLERRYGSTWR
RSAKERTLFTKRMTIIKRIIDIKDDPSKYGLSLPENKISRNQAIKVVENIRLGNNTFK
GHHCRLSMSQLYEYFSKKMDKLEDYSLTLKRRGKPRRIFLLEEREARLSLQQLHSIPN
SSTGTPEHDQDT"
gene complement(461753..462148)
/gene="Med11"
/locus_tag="P301_M11896"
mRNA complement(461753..462148)
/gene="Med11"
/locus_tag="P301_M11896"
/product="Med11p"
CDS complement(461753..462148)
/gene="Med11"
/locus_tag="P301_M11896"
/note="Similar to YMR112C 'Subunit of the RNA polymerase
II mediator complex; associates with core polymerase
subunits to form the RNA polymerase II holoenzyme;
essential protein'"
/codon_start=1
/product="Med11p"
/protein_id="EWG89396.1"
/translation="MQVLNTKSETKQENETMQPPYIQERLKSLNDIETQLCSMLQEAS
QVTFIFGELKRGNESVKPQFENHVKQFYERLDKSTTQLRKEIQLLDENVGTRLLPINV
NKKALGQDTEKMEEQLDLLSAILDPSKSK"
gene 462651..463934
/gene="Fol3"
/locus_tag="P301_M11906"
mRNA 462651..463934
/gene="Fol3"
/locus_tag="P301_M11906"
/product="Fol3p"
CDS 462651..463934
/gene="Fol3"
/locus_tag="P301_M11906"
/note="Similar to YMR113W 'Dihydrofolate synthetase,
involved in folic acid biosynthesis; catalyzes the
conversion of dihydropteroate to dihydrofolate in folate
coenzyme biosynthesis'"
/codon_start=1
/product="Fol3p"
/protein_id="EWG89397.1"
/translation="MAIELGLSRITKLLEHLGNPQNSLRVLHIAGTNGKGSVCTYLSS
VLQQKSYQIGKFTTPHLVHVTDSITINNKPIPLERYQNIRLQLEALNKSHSLKCTEFE
LLTCTAFKYFYDVQCQWCVIEVGLGGRLDATNVIPGANKACCGITKISLDHESFLGNT
LSDISKEKAGIITEGVPFTVIDGTNEASVINVVKERCKALGSELSVTDSQLNGNMIDT
NSWGCFDLAKLPLNGEYQIFNLRVAMGMLDYLQMNELIDITKNEVSTRLAKVDWPGRL
YRMDYRFDKVSNRTVPILMDGAHNGSAAVELVKYLRKEYGNQPLTFVMAVTHGKNLEP
LLQPLLRPIDQVILTRFNNVEGMPWIHATDPEEIKDFILTQGYTKEIVIENDLHQVLP
SLAHVSDEQRRPIVVCGSLYLCGELLRIHNSHLRN"
gene complement(463995..465101)
/locus_tag="P301_M11911"
mRNA complement(463995..465101)
/locus_tag="P301_M11911"
/product="hypothetical protein"
CDS complement(463995..465101)
/locus_tag="P301_M11911"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89398.1"
/translation="MCGRFALAYDSGDLPQLLRDWNLPVNTPKGASSNSQHPHDEEDT
KDQPTVSKDIFKASYNISPTNYSAVYRPDTKAIQFMRWGLVPFWTKDVSQFKTYRTFN
ARLENLQESKMWMRPCEKKRCAVLMSGYFEWKTVGKKKTPYFISRRDGRLMFVAGMYD
YVEKEDLYTFTIITAQGPRELEWLHERMPCVLEPGTESWDAWMDVDKTTWSTEELVKL
LKPDYDESKLQFYQVTDDVGKTTNTGERLIKPLLKEDSDMFSVKREKEEALLENDNEQ
GIDNRGVKGDKSLKGEDVFNQKKSLKRNTYDGLKKNEEQEKTTLPEEGSIGDRVKREE
ANLSPKREGNREKRNIVNMLGNQKDSRGKKKIKK"
gene 465356..466860
/gene="Fmp24"
/locus_tag="P301_M11916"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(467107..468339)
/gene="Asc1"
/locus_tag="P301_M11921"
mRNA complement(join(467107..467529,467803..468339))
/gene="Asc1"
/locus_tag="P301_M11921"
/product="Asc1p"
CDS complement(join(467107..467529,467803..468339))
/gene="Asc1"
/locus_tag="P301_M11921"
/note="Similar to YMR116C 'G-protein beta subunit and
guanine nucleotide dissociation inhibitor for Gpa2p;
ortholog of RACK1 that inhibits translation; core
component of the small (40S) ribosomal subunit; represses
Gcn4p in the absence of amino acid starvation'"
/codon_start=1
/product="Asc1p"
/protein_id="EWG89399.1"
/translation="MASNEVLVLRGTLEGHNGWVTSLATSAGQPNLLLSASRDKTLIS
WKLTGDDQKFGVPVRSFKGHSHIVQDCTLTADGAYALSASWDKTLRLWDVATGETYQR
FVGHKSDVMSVDIDKKASMIISGSRDKTIKVWTIKGQCLATLLGHNDWVSQVRVVPNE
KADDDSVTIISAGNDKMVKAWNLNQFQIEADFIGHNSNINTLTASPDGTLIASAGKDG
EIMLWNLAAKKAMYTLSAQDEVFSLAFSPNRYWLAAATATGIKVFSLDPQYLVDDLRP
EFAGYSKAAEPHAVSLAWSADGQTLFAGYTDNVIRVWQVMTAN"
gene complement(467635..467723)
/locus_tag="P301_M11926"
ncRNA complement(467635..467723)
/ncRNA_class="snoRNA"
/locus_tag="P301_M11926"
/product="SNR24"
gene complement(468902..469543)
/gene="Spc24"
/locus_tag="P301_M11936"
mRNA complement(468902..469543)
/gene="Spc24"
/locus_tag="P301_M11936"
/product="Spc24p"
CDS complement(468902..469543)
/gene="Spc24"
/locus_tag="P301_M11936"
/note="Similar to YMR117C 'Component of the evolutionarily
conserved kinetochore-associated Ndc80 complex
(Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome
segregation, spindle checkpoint activity and kinetochore
clustering'"
/codon_start=1
/product="Spc24p"
/protein_id="EWG89400.1"
/translation="MSQKDNLLDNPVEFLKEVRESFDIQQDVDAMKRIRHDLDVIKEE
SEARISKEHSKVSESNKKLNAERINVAKLEGDLEYTNEESNEFGSKDELVKLLKDLDG
LERNIVSLRSELDEKMKLYLKDSEIISTPNGSKIKAKVIEPELEEQSAVTPEANENIL
KLKLYRSLGVILDLENDQVLINRKNDGNIDILPLDNNLSDFYKTKYIWERLGK"
gene complement(469796..470386)
/locus_tag="P301_M11941"
mRNA complement(469796..470386)
/locus_tag="P301_M11941"
/product="hypothetical protein"
CDS complement(469796..470386)
/locus_tag="P301_M11941"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89401.1"
/translation="MKATIQRVTSLFGVPRASVFVPRISTPFILHSYISNGRMVLFSK
EFHNGRVSKSDLWSSNKEEELLVSQRKKRPISPHLTVYEPEMSWYLSSLHRISGVLLA
LGFYAFTITLGVTTIMGMDTTFQDLNKWYHEKMPKWSQWVAKGSAAYLFAFHFGNGIR
HLIWDMGYELTNRGVIKTGSIVLAGTLVLGTYLLAQ"
assembly_gap 471279..472613
/estimated_length=1335
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 472927..474801
/gene="Asi1"
/locus_tag="P301_M11951"
mRNA 472927..474801
/gene="Asi1"
/locus_tag="P301_M11951"
/product="Asi1p"
CDS 472927..474801
/gene="Asi1"
/locus_tag="P301_M11951"
/note="Similar to YMR119W 'Putative integral membrane E3
ubiquitin ligase; acts with Asi2p and Asi3p to ensure the
fidelity of SPS-sensor signalling by maintaining the
dormant repressed state of gene expression in the absence
of inducing signals'"
/codon_start=1
/product="Asi1p"
/protein_id="EWG89402.1"
/translation="MNSSTSSENVFINSFSYLNQTSQAVISGNSTFANVINFPYRLGL
SFIGAVNLQYEQTVKSEEIPPTLRSVFDTIGFFFSPYAIFCFVIAIVLNRFVVFYAVL
NNGSRRTLPLWLSNVFHISAVVVLAMVSLGPLTLGKDFKILGDPAFAQEKFLLNIFYA
FAYSYCVETIFTIMRNSSPLEGTDYSLFELSIQFYTMTNNNTKLLDSPDYIIDCSMAI
LSRILIHLVEIFRLRNYRLLFSTIMNLCHICYLGIRVKQGGWKSLPFSVKFRHFPKLF
SVSIICLSLLIFKLSCLIRWDPFGKSRNSCELLQFYPLSRNWKKYLNYTGEEDFSAMA
TKFALLLCSGTELMEKGIRREFPAINIPDNVNEKFFISGYLNELSKPYKENTSISFPK
KNSSILKQRFFLMFPKSIIWIMKKLVGQVFFGFRDNKDEDIPDNDPSKMLKITKTNSL
NNSAGHKEDIELELLNTSDDEYSEDYEPSEVESLGDSDEENLEEDSLIFNETRDALLD
LFSSEDNEVHTDYNWIMSTSRILQQKLLSDKTLTRASILDTELSEVDETFGTESDFDL
SCAVCKVNERNTVLWPCRCFAICEDCRISLGLRGFSTCVCCRSKVHGYCKVHPVSDSK
"
gene 474590..474949
/locus_tag="P301_M11956"
mRNA 474590..474949
/locus_tag="P301_M11956"
/product="hypothetical protein"
CDS 474590..474949
/locus_tag="P301_M11956"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89403.1"
/translation="MRLLVQKVILIYLAPYAKSMKEIQYYGPADVLPFARIAEFLWVY
VGSALAYVVGVKFMGTVRFILFQIVNNSLRTFAWYLKEVFRLGALALITIARAFCVGH
INIIIINQVFFFGKCSN"
gene complement(475095..476873)
/gene="Ade17"
/locus_tag="P301_M11961"
mRNA complement(475095..476873)
/gene="Ade17"
/locus_tag="P301_M11961"
/product="Ade17p"
CDS complement(475095..476873)
/gene="Ade17"
/locus_tag="P301_M11961"
/note="Similar to YMR120C 'Enzyme of 'de novo' purine
biosynthesis containing both
5-aminoimidazole-4-carboxamide ribonucleotide
transformylase and inosine monophosphate cyclohydrolase
activities, isozyme of Ade16p; ade16 ade17 mutants require
adenine and histidine'"
/codon_start=1
/product="Ade17p"
/protein_id="EWG89404.1"
/translation="MANYTKTAILSVYDKTGLLDLARGLIEKNVRILASGGTARMIRD
AGFPIEDVSAITHAPEMLGGRVKTLHPAVHGGILARDIDSDEKDLKEQHIEKVDYVVC
NLYPFKETVAKVGVTIPEAVEEIDIGGVTLLRAAAKNHARVTILSDPKDYSEFLSELS
SNGEISQDLRNRLALKAFEHTADYDAAISDFFRKQYSEGQAQITLRYGANPHQKPAQA
YVSQQDSLPFKVLCGSPGYINLLDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVG
IPLSDVEKQVYFVADIENLSPLACAYARARGADRMSSFGDWIALSNIVDVPTAKIISR
EVSDGVIAPGYEPEALAILSKKKGGKYCILQIDPNYVPEAVERRQVYGVTLEQKRNDA
IINQSTFKEIVSQNKNLTEQAIIDLTVATIAIKYTQSNSVCYARNGMVVGLGAGQQSR
IHCTRLAGDKADNWWFRQHPRVLEIKWAKGVKRPEKSNAIDLFVTGQIPTEEPELSEY
QSKFEEIPKPFTPEERKEWLSKLTNVSLSSDAFFPFPDNVYRAVKSGVKYIAAPSGSV
MDKVVFSAADSFDLVYVENPIRLFHH"
gene complement(477328..477942)
/gene="Rpl15b"
/locus_tag="P301_M11966"
mRNA complement(477328..477942)
/gene="Rpl15b"
/locus_tag="P301_M11966"
/product="Rpl15bp"
CDS complement(477328..477942)
/gene="Rpl15b"
/locus_tag="P301_M11966"
/note="Similar to YMR121C 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl15Ap and
has similarity to rat L15 ribosomal protein; binds to 5.8
S rRNA'"
/codon_start=1
/product="Rpl15bp"
/protein_id="EWG89405.1"
/translation="MGAYKYLEELERKKQSDVLRFLQRVRVWEYRQKHVIHRAARPTR
PDKARRLGYKAKQGFVIYRVRVRRGNRKRPVPKGATYGKPTNQGVNELKYQRSLRATA
EERVGRRAANLRVLNSYWVNQDSTYKYFEVILVDPQHKAIRRDARYNWICNPVHKHRE
ARGLTATGKKSRGINKGHKFNNTKAGRRKTWKRQNTLSLWRYRK"
gene 478910..479164
/locus_tag="P301_M11991"
mRNA 478910..479164
/locus_tag="P301_M11991"
/product="hypothetical protein"
CDS 478910..479164
/locus_tag="P301_M11991"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89406.1"
/translation="MASSTSTSASASSSIKTNSALVSNNVVAASSVSATSTASSSAAK
NTTSSSKNAAPGMVANPVSSKYGIIMAAFAAVSFVLGTGI"
gene 481317..481685
/gene="Pkr1"
/locus_tag="P301_M12006"
mRNA 481317..481685
/gene="Pkr1"
/locus_tag="P301_M12006"
/product="Pkr1p"
CDS 481317..481685
/gene="Pkr1"
/locus_tag="P301_M12006"
/note="Similar to YMR123W V-ATPase assembly factor,
functions with other V-ATPase assembly factors in the ER
to efficiently assemble the V-ATPase membrane sector (V0)"
/codon_start=1
/product="Pkr1p"
/protein_id="EWG89407.1"
/translation="MANFFVRLWESVFEPGTSPQLIIATHVSFVALLLTLIWLIYATN
GNIHFYALFCISLLLWITVIWFINELSHVKLKDNDELDKDANKKDDSAIKEDSEDKQE
SGKSTSTARRTQAQSRSRKA"
gene 482181..485011
/locus_tag="P301_M12011"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 482652..482731
/estimated_length=80
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 485198..485265
/estimated_length=68
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 485266..488171
/gene="Sto1"
/locus_tag="P301_M12016"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 487304..487558
/estimated_length=255
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 488305..488341
/estimated_length=37
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(488488..489516)
/gene="Dlt1"
/locus_tag="P301_M12021"
mRNA complement(488488..489516)
/gene="Dlt1"
/locus_tag="P301_M12021"
/product="Dlt1p"
CDS complement(488488..489516)
/gene="Dlt1"
/locus_tag="P301_M12021"
/note="Similar to YMR126C Protein of unknown function,
mutant sensitive to 6-azauracil (6AU) and mycophenolic
acid (MPA)"
/codon_start=1
/product="Dlt1p"
/protein_id="EWG89408.1"
/translation="MSGFAKLKSWLYKASLFVSLILLIGFSVVLPIDSIAQASKSENN
AFNTFIVVGALVVFGVFCIFIIIGRMLFHKSCLKDIPRRYIPITPADLPHRSSREAVL
QNMERSKELTILLKKPKDPVIHDGLEPPRRCDYPLDEKLFPEYLNYADCIKSLTDRLK
YHGLFLNNLDVRMNLEDTFADVVNSQFVNHNANKIQLEKAKEFIDLYETIRFSGKDVT
RDQFIKFVKFCLYFGEVSLTRDTSFANLHNFRLNGSSNNIGRTESKYSINPFDENEYA
QDDMHYFPEPPTHLVRESSISTVARHVSSGVDLTNSEEHPLDTDSDCNALRLKLSKAD
SYRSVIRH"
gene complement(490056..491072)
/gene="Sas2"
/locus_tag="P301_M12031"
mRNA complement(490056..491072)
/gene="Sas2"
/locus_tag="P301_M12031"
/product="Sas2p"
CDS complement(490056..491072)
/gene="Sas2"
/locus_tag="P301_M12031"
/note="Similar to YMR127C 'Histone acetyltransferase (HAT)
catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p),
which acetylates free histones and nucleosomes and
regulates transcriptional silencing; member of the
MYSTacetyltransferase family'"
/codon_start=1
/product="Sas2p"
/protein_id="EWG89409.1"
/translation="MARSLSQSLTATTQKLKGKKNGGKGKNKPSAKIKKTQXXXXXGI
LNERNIRQIQFGLNKKFSTWYGSAVYFDPETKRLGCSETKGQLSSVSNSQYWLDTLFV
CEYCFKYTDDQTRFVGHVASCPFQYRVPGKIKYKSPEYTIRRVKGSKYQLFCQCLCLF
TKLYLDNKSMYFKVDHYEFYIVYETGSTKPMGFFSKDLVSYQQNNLACILIFPPYQRR
GLGLLLIEFSYKLSQLEGVISGPEVPLSPFGLIGYLKYWSQILCWHLIEGDLAHYDKV
TLEDLSIVTGMRVNDVILTLKHLNCIGENNQIYLQSLNSWLKLHGTKRNWFKLKDEYL
LIDD"
assembly_gap 490948..490960
/estimated_length=13
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 491422..495225
/gene="Ecm16"
/locus_tag="P301_M12036"
mRNA 491422..495225
/gene="Ecm16"
/locus_tag="P301_M12036"
/product="Ecm16p"
CDS 491422..495225
/gene="Ecm16"
/locus_tag="P301_M12036"
/note="Similar to YMR128W Essential DEAH-box ATP-dependent
RNA helicase specific to the U3 snoRNP, predominantly
nucleolar in distribution, required for 18S rRNA
synthesis"
/codon_start=1
/product="Ecm16p"
/protein_id="EWG89410.1"
/translation="MGTYRKRFNEKARSGHMAKLKELKRIRNKQFTRQDENDERVENP
DSAPAESSTTEPNANAEILEPLTEEEKKMKKRKLQELFTPKXXXXXXXXXXXXXXXXX
XXXXREERKTIIGKLQDYKIDTSLLTSSKRLGEGRQTKKEEFKEALSLERQGRGNEQT
NEILYEEYEPKVWDEYGEGGSSEDDDGEDDFEASFGSMPKPTDNEEKKSSGFIDHRPA
KFGGSGLSFGFSNIKVINKESKTPKKKYNWRQRVEMEELKKHGKEDEMDFDTTSEDDD
EEEDQEEEDKMHPSENPLEEVESADSETGSEKFDQNDVANEFKDWANQEIKKLEGRDQ
ELVTPTLNIDYKPIIRKEDLDDGLQEAYVPINENSTRKAFYVEVSRSDEIQKARIQLP
VFGEEHKIMEAIHHNDVVIICGETGSGKTTQVPQFLYEAGFGAEDSPDYPGMVGITQP
RRVAAVSMAERVANELGDHGHKVGYQIRFDSTAKEDTKVKFMTDGVLLREMMHDFKLT
KYSSIIIDEAHERNINTDILIGMLSRCVRLRAKLHKENPIEHKKLKLIIMSATLRVSD
FSENKTLFPIAPPVLQVDARQFPVSIHFNRRTAFNYTDEAFRKTCKIHQKLPPGAILV
FLTGQQEITHMVKRLRKEFPFKKNSKYNKDLETPVSKMGINSKTTDLEAEDIDFSVQV
IDQDKFKSAIRYEEDEGNSGNGEDEEDEEEEGFEEVLTEGQTANDPLYVLPLYSLLPT
KEQMRVFQKPPQGSRLCIVATNVAETSLTIPGVRYVVDSGRSKERKYNESNGVQSFEV
GWVSKASANQRSGRAGRTGPGHCYRLYSSAVFEHDFEQFSKPEILRMPVESIVLQMKS
MAIHNIINFPFPTPPDRVALSKAIQLLQYLGALDNKEMITEDGKKMSLFPLSPRFSKM
LLVSDEKACLPYIVAIVSALSVGDPFINEFELGINEISRKQNPDENLDDKIREHDEST
PGMDPELKKELRSKFYKSRSQFSKLDKFSDVFRLLSVVSAMDYVPKEQKEIFMKKNFL
RGKLMEEIVKLRKQLMYIIKSNTSKENIAVVIRNEDLKSDIPSVIQIKLLKQMICAGF
VDHVAVRADVLFPDDAKITNRTSIINIPYIPVLATRTPNIEDCFVYIHPTSILNNLGE
MPPKYMLYYSLHLGGNNKTRMNTLCDIASTPLANIARKGLLLTYSKPLTGQGLKTVNL
SPTERYCYVVPRFGSTVDNDLKIGWDLNPIAVHQKKKKGQWTVIKFITRKGFQTITGE
EKEKK"
assembly_gap 491679..491738
/estimated_length=60
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 495530..499543
/gene="Pom152"
/locus_tag="P301_M12041"
mRNA 495530..499543
/gene="Pom152"
/locus_tag="P301_M12041"
/product="Pom152p"
CDS 495530..499543
/gene="Pom152"
/locus_tag="P301_M12041"
/note="Similar to YMR129W 'Nuclear pore membrane
glycoprotein; may be involved in duplication of nuclear
pores and nuclear pore complexes during S-phase;'"
/codon_start=1
/product="Pom152p"
/protein_id="EWG89411.1"
/translation="MEHRYNVFNDTPRGNHWMGSSVSGSPRPSYSSRPNVNTTRRFQY
SDDEPAEKIRPLRSRSFKSTESNISDEKSRISERDSKDRYINGDKKVDIYSLPLISTD
VLEISKQRTFAVILFLIIQCYKIYDLVILKSGLPLSGLLFKNYRFNFISKYFIIDSFF
LYVLPSFNIPRLTFKPWVVYLQILAMLLLNIFISSDHEFVLISLIMTTWRKLYTKELS
VTGSAINHHRIFDSSAHFKGALTIKILPENTAMFNPLHESYCLPMDTNLFKINSIDVP
IRINSTEEIEYIELEYRDLYTNSVELRSLNKKDFKIIDNPKSFLKKDQSVLKSHSNDF
EEGSTIRYLAVTLQDIGFYQIKKIVDSKKLNLKIHQSHLVVPYCPIASITGTGSNDRC
IGDSDNVSFEIQGVPPMKLAYSKIVNGQTFSYVDSSLQPEYFESPLQSSKSKQSFTQG
ELNDLKWGRNQPVNINLDSSITQDGKFAYKIDKITDGLGNVVDFTSLPEELKKRYDLS
YNFNVHEVPRAALEERFDPKSPTKRSIAIVFEEIKNWISDIPYVISLSYTDAQDKSKK
IMNVTTDSLTKVLQADLPGSYNLEYIESKFCPGEIVGKSNVLVTMPVAPTMEVKSFPI
LDQCVGQVGLNFELSFTGAPPYYYNTKIYKLENGERKLYDAKRYTSEGTRNRFSYSPP
KEGNYEIVFDTVSNKLFTEPIKLEPVKEYTFKTSMRVKPSASLKLHHDLKLCLGDHSS
VPVALKGQGPFTLTYDIIETFSSKRKTFEIKEIKTNEYVIKTPVFTTGGDYILSLVSI
KDSTGCVVGLSQPDAKIQVRRDIPSAAFNFFEPIKEAKIKHGSVTEIPLKLSGEGPFT
VKFKHMDYDGNIVKEFENKFQNSYKPALKVSKEGLYQLVDIRDSSCQGNVIYRNSLYK
VSFLEKPKFAIQDNHHITKVTENLFSKEEVCQGMEGTVDLALFGSPPFILEYDLMAPN
GHISTKKIQVATKYASLKLPNQIPGEYITTIKAIFDGNYGESDIHFREHQSELIIKQT
VHPIPDVAFADGGKTLRACAANVDQISFLEPINLKFLQGESPFSITFSVYHESTSRTD
QYTIDNIDSENFAFEKLYEGMKLGNHAITIDSVVDANGCVNSLISGPRNQILVSITDA
PKIHILDPSTEYCVGDYVAYQLNGVAPFMIKYEFNGIPLKSKERSSQFVRLASEPGII
SITSLQDSSSQCIVDFTNPKLKSEFDDLSLNIHPIPSVTVSQGNYVTEDIREGDQAEV
IFSFEGTPPFSLTYVRTEETDGKHGKRRSQVVETHKVTDIYSHEYKVITSLQGTYEAI
EITDAYCFAKNDLFFNN"
gene 499845..500753
/locus_tag="P301_M12046"
mRNA 499845..500753
/locus_tag="P301_M12046"
/product="hypothetical protein"
CDS 499845..500753
/locus_tag="P301_M12046"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89412.1"
/translation="MTYPKRIPINAWSEVHRVARPLIITFDAYNTLYATKLPVMEQYC
IVGRKYGIKANPSTLTNNFPHVFKKLKEDYPQYGKYSGIKPEQWWSILIRNVFAPNEI
PDEMINEILMRFEGFDSYFVYPDLIKFLKDLKSRHPDVILGIVSNTDPIFYKLLKNIG
LFETFSGHIYLSYELNLAKPDRAIFQYALDDIISKQPHLLEKYTREEILQHCFHIGDE
LKNDLEGAEAAGWTGILLDRNDKYGFLSNSISKPMRDEYKLSIDKIDNNSINTWEAST
KQTDTLQLSERKYVVSNLEVLEELFP"
gene complement(500889..502424)
/gene="Rrb1"
/locus_tag="P301_M12051"
mRNA complement(500889..502424)
/gene="Rrb1"
/locus_tag="P301_M12051"
/product="Rrb1p"
CDS complement(500889..502424)
/gene="Rrb1"
/locus_tag="P301_M12051"
/note="Similar to YMR131C 'Essential nuclear protein
involved in early steps of ribosome biogenesis; physically
interacts with the ribosomal protein Rpl3p'"
/codon_start=1
/product="Rrb1p"
/protein_id="EWG89413.1"
/translation="MSKRSIEVNEEQDRVVSAKTESHSVPAIPASEEQDAPMNDLEEQ
LSDEFDSDGEIIEIDGDDEINDEDDLRKKQEEAETLVQKDQSEGNKEKIQELYLPHMS
RPLGPDEVLEADPTVYEMLHNVNMPWPCLTLDVIPDTLGSERRNYPQSILLTTATQSS
RKKENELMVLALSNLAKTLLKDDNEGEDDEEDDEDDVDPVIENENIPLRDTTNRLKVS
PFAISNQEVLTATMSENGDVYIYNLAPQSKAFSTPGYQIPKSAKRPIHTVKNHGNVEG
YGLDWSPLIKTGALLSGDCSGQIYFTQRHTSRWVTDKQPFTVSNNKSIEDIQWSRTES
TVFATAGCDGYIRIWDTRSKKHKPAISVKASNTDVNVISWSDKIGYLLASGDDNGTWG
VWDLRQFTPSNADAVQPVAQYDFHKGAITSIAFNPLDESIVAVGSEDNTVTLWDLSVE
ADDEEIKQQAAETKELQEIPPQLLFVHWQKEVKDVKWHKQIPGCLVSTGTDGLNVWKT
ISV"
gene complement(502669..503031)
/gene="Jlp2"
/locus_tag="P301_M12056"
mRNA complement(502669..503031)
/gene="Jlp2"
/locus_tag="P301_M12056"
/product="Jlp2p"
CDS complement(502669..503031)
/gene="Jlp2"
/locus_tag="P301_M12056"
/note="Similar to YMR132C Protein of unknown function,
contains sequence that closely resembles a J domain
(typified by the E. coli DnaJ protein)"
/codon_start=1
/product="Jlp2p"
/protein_id="EWG89414.1"
/translation="MPQCTILITPWHNLRKNRYMNPGEVSFKSLRQCRKMECGARDNK
ILNRLTKTRVELFNNVEATLNEAKKTKNGDFFVNYIESNRSNLIEEEKLRKVAKKNQK
KKNKQSKDEVTDDMQLEV"
assembly_gap 503098..503934
/estimated_length=837
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<503935..504440)
/gene="Erg29"
/locus_tag="P301_M12061"
mRNA complement(<503935..504440)
/gene="Erg29"
/locus_tag="P301_M12061"
/product="Erg29p"
CDS complement(<503935..504440)
/gene="Erg29"
/locus_tag="P301_M12061"
/note="Similar to YMR134W 'Protein of unknown function
that may be involved in iron metabolism; mutant bm-8 has a
growth defect on iron-limited medium that is complemented
by overexpression of Yfh1p; shows localization to the ER;
highly conserved in ascomycetes'"
/codon_start=1
/product="Erg29p"
/protein_id="EWG89577.1"
/translation="MSLKDRYLNLELKLINKLQELPYVHQFIHDRISGRITLFLIVVG
TLAFFNELYITIEMSLLQKNTSEELERGRIDESLKLHRMLVSDEYHGKEYKDEKSGIV
IEEFEDRDKFFAKPVFVSELDVECNVIVDGKELLSTPLKFHVEFSPEDYENEKRPEFG
TTLRVLRL"
assembly_gap 506073..506104
/estimated_length=32
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(506420..507787)
/gene="Gid8"
/locus_tag="P301_M12076"
mRNA complement(506420..507787)
/gene="Gid8"
/locus_tag="P301_M12076"
/product="Gid8p"
CDS complement(506420..507787)
/gene="Gid8"
/locus_tag="P301_M12076"
/note="Similar to YMR135C 'Protein of unknown function,
involved in proteasome-dependent catabolite inactivation
of fructose-1,6-bisphosphatase; contains LisH and CTLH
domains, like Vid30p; dosage-dependent regulator of
START'"
/codon_start=1
/product="Gid8p"
/protein_id="EWG89415.1"
/translation="MTISTLSNETTKSGSCSGQGKNGGKDFTYGKKCFTKEEWKEQVA
KYSAMGELYANKTIHYPLKIQPNSSGGSQDEGFATIQTTPIEPTLPRLLLNYFVSMAY
EDSSIRMAKELGFIRNNKDIAVFNDLYKIKERFHIKHLIKLGRINEAMEEINSIFGLE
VLEETFNATGSYTGRTDRQQQQQQQQFDIDGDLHFKLLLLNLIEMIRSHHQQENITKD
SNDFILNLIQYSQNKLAIKASSSVKKMQELELAMTLLLFPLSDSADSGSIKLPKSLQN
LYSISLRSKIADLVNEKLLKFIHPRIQFEISNNNSKFPDLLNSDKKIITQNFTVYNNN
LVNGSNGTKITHISSDQPINEKMSSNEVTAAANSVWLNQRDGNVGTGSAATTFHNLEN
KNYWNQTSELLSSSNGKEKGLEFNNYYSSEFPYEPRLTQIMKLWCWCENQLHHNQIGV
PRVEN"
gene 507643..508175
/locus_tag="P301_M12081"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 508928..510610
/gene="Gat2"
/locus_tag="P301_M12091"
mRNA 508928..510610
/gene="Gat2"
/locus_tag="P301_M12091"
/product="Gat2p"
CDS 508928..510610
/gene="Gat2"
/locus_tag="P301_M12091"
/note="Similar to YMR136W 'Protein containing GATA family
zinc finger motifs; similar to Gln3p and Dal80p;
expression repressed by leucine'"
/codon_start=1
/product="Gat2p"
/protein_id="EWG89416.1"
/translation="MQAPNIYPFSQTQPQALPGFTYGPPQLVFDHSAPRVDPLHSTVT
INSPLPLQHYNGPNAHINSANNNYAYYYHHPNNNDNNNHSNNTIKNNNINSVLPAVNI
QISNNSHYRNTHQIPSAPQRLVSIIPDPHMPPNISHFQLNNIHPQMHAPVATDIHFQQ
VPVYNKTNNGIGTDNINNDKPVNSNQNEVLDNIDERSCHEINRVVSFSKHFENNELTT
TANDLNIQSTMDELAKLKSLSNSTHFRQSIATQNFHSLQNHITTIENRLASLLTDRQQ
EQQQLKQQESEKESSSPFSNKIKLPSLQELTDSISTQHLPTFYDNKRHASDTDLKSST
LHGPLYHRHAFLSTSSSSPSPTAGSVPLQKLQVPRQDDPNDKKMNISSSPFNSITYIP
NTTLSPMVQTQLKNLTTSNLNTKKKNNRGRPRAIQRQPTLTTSSHFINNSNPGAAAVS
TTTPAANSDEKNPNAKKIIEFCFHCGETETPEWRKGPYGTRTLCNACGLFYRKVTKKF
GSKSSNLLLRYRRSIDLANDRRIPDFITIPNRFIHDMDNDQTLDSEYNTILQ"
gene complement(510707..512692)
/gene="Pso2"
/locus_tag="P301_M12096"
mRNA complement(510707..512692)
/gene="Pso2"
/locus_tag="P301_M12096"
/product="Pso2p"
CDS complement(510707..512692)
/gene="Pso2"
/locus_tag="P301_M12096"
/note="Similar to YMR137C 'Nuclease required for a
post-incision step in the repair of DNA single and
double-strand breaks that result from interstrand
crosslinks produced by a variety of mono- and
bi-functional psoralen derivatives; induced by
UV-irradiation'"
/codon_start=1
/product="Pso2p"
/protein_id="EWG89417.1"
/translation="MSRKSIVQIRRSEVKRKRSSTASSTSEGKTLHKNTHTSSKRQRT
LTEFNIPTSSNLPVRSSSYSFSRFSCSTSNKNTEPVIINDDDHNSICLEDTAKVEITI
DTDEEELVSLHDNEVSAIENRTEDRIVTELEEQVNVKVSTEVIQCPICLENLSHLELY
ERETHCDTCIGSDPSNMGTPKKNIRSFISNPSSPAKTKRDIATSKKPTRVKLVLPSFK
IIKFNNGHEIVVDGFNYKASETISQYFLSHFHSDHYIGLKKSWNNPDENPIKKTLYCS
KITAILVNLKFKIPMDEIQILPMNKRFWITDTISVVTLDANHCPGAIIMLFQEFLANS
YDKPIRQILHTGDFRSNAKMIETIQKWLAETANETIDQVYLDTTYMTMGYNFPSQHSV
CETVADFTLRLIKHGKNKTFGDSQRNLFHFQRKKTLTTHRYRVLFLVGTYTIGKEKLA
IKICEFLKTKLFVMPNSVKFSMMLTVLQNNENQNDMWDESLLTSNLHESSVHLVPIRV
LKSQETIEAYLKSLKELETDYVKDIEDVVGFIPTGWSHNFGLKYQKKNDDDENEMSGN
TEYCLELMKNDRDNDDENGFEISSILRQYKKYNKFQVFNVPYSEHSSFNDLVKFGCKL
KCSEVIPTVNLNNLWKVRYMTNWFQCWENVRKTRAAK"
gene 512884..513459
/gene="Cin4"
/locus_tag="P301_M12101"
mRNA 512884..513459
/gene="Cin4"
/locus_tag="P301_M12101"
/product="Cin4p"
CDS 512884..513459
/gene="Cin4"
/locus_tag="P301_M12101"
/note="Similar to YMR138W 'GTP-binding protein involved in
beta-tubulin (Tub2p) folding; isolated as mutant with
increased chromosome loss and sensitivity to benomyl;
regulated by the GTPase-activating protein, Cin2p, the
human retinitis pigmentosa 2 (RP2) homolog'"
/codon_start=1
/product="Cin4p"
/protein_id="EWG89418.1"
/translation="MGLLSIIRKQKLRDKEIRCLILGLDNSGKSTIVNKLLPKDEQNN
DGIMPTVGFQIHSLMIKDVTISLWDIGGQRTLRPFWDNYFDKTQAMIWCIDVSLSMRF
DETLQELKELINRDENRIGYECAVIVVLNKIDLVEDKSELHRRCLLVESELKCLFKPD
IRIELVKCSGVTGEGIDNLRDRLVESCHFTQ"
gene 513854..514966
/gene="Rim11"
/locus_tag="P301_M12106"
mRNA 513854..514966
/gene="Rim11"
/locus_tag="P301_M12106"
/product="Rim11p"
CDS 513854..514966
/gene="Rim11"
/locus_tag="P301_M12106"
/note="Similar to YMR139W 'Protein kinase required for
signal transduction during entry into meiosis; promotes
the formation of the Ime1p-Ume6p complex by
phosphorylating Ime1p and Ume6p; shares similarity with
mammalian glycogen synthase kinase 3-beta'"
/codon_start=1
/product="Rim11p"
/protein_id="EWG89419.1"
/translation="MNIQSNNSPNLSNNIVSKQVYYAHPPPTIDPNDPVQISFPTTEV
VGHGSFGVVFATVIQETNEKVAIKKVLQDKRFKNRELEIMKMLSHINIIDLKYFFYER
DSQDEIYLNLILEYMPQSLYQRLRHFVHQRTPMSRLEIKYYMFQLFKSLNYLHHFANV
CHRDIKPQNLLVDPETWSLKLCDFGSAKQLKPTEPNVSYICSRYYRAPELIFGATNYT
NQIDIWSSGCVMAELLLGQPMFPGESGIDQLVEIIKILGTPSKQEICSMNPNYMEHKF
PQIKPIPLSRVFKKEDDQTVEFLADVLKYDPLERFNALQCLCSPYFDELKLDDGKINQ
ITTDLKLLEFDENVELGHLSPDELSSVKKKLYPKSK"
assembly_gap 515307..515327
/estimated_length=21
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 515450..516919
/gene="Sip5"
/locus_tag="P301_M12111"
mRNA 515450..516919
/gene="Sip5"
/locus_tag="P301_M12111"
/product="Sip5p"
CDS 515450..516919
/gene="Sip5"
/locus_tag="P301_M12111"
/note="Similar to YMR140W 'Protein of unknown function;
interacts with both the Reg1p/Glc7p phosphatase and the
Snf1p kinase'"
/codon_start=1
/product="Sip5p"
/protein_id="EWG89420.1"
/translation="MGNVPGKIDQEDSFNDVRPDSSYNTTSSNSVIKQYDEEASSRVR
TRRTTSLVNNILNGNNARTKTGSHLSSTSRRKTSREKELAKEAHAKQLVVRCSETVDG
GFLAPFGCYSFEKLDYDATVVKNLIIKRKLAPFYTPLQDFDESWTRDELIKIVDGLPL
HDTFDENLEEFEDVPIGNLRKSTFNELIDKSLSKKEQRRMHAKIFRARLYKKRILWQE
NENETFLERKLEMKRIGSKSSNVEDNTSSQPRKNYHLPSDDLKYTLYKNGSECPICFL
YFPGPFNYSKCCQQPICTECFVQIKRADPHFPHDEVDPTEPQTNDSEKDPNLLTSEPA
NCPYCATASFSITYQPPTNRETGIGGMPADSYVYKDAAISRADGGQPNISAITSDTIR
PDWEIKLNKERARLMRRSANATAIHISNRLIDPSHSRRRNTSHSITPIHDESTSASRS
PEPTINELEDQMVREAIRLSLEDQDNRKKSKNRNTSLRP"
gene complement(517942..>518844)
/gene="Rpl13b"
/locus_tag="P301_M12121"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 518845..519223
/estimated_length=379
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 519663..520638
/gene="Rps16a"
/locus_tag="P301_M12131"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 521097..522126
/locus_tag="P301_M12136"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 521433..521452
/estimated_length=20
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(522355..524211)
/gene="Nde1"
/locus_tag="P301_M12146"
mRNA complement(522355..524211)
/gene="Nde1"
/locus_tag="P301_M12146"
/product="Nde1p"
CDS complement(522355..524211)
/gene="Nde1"
/locus_tag="P301_M12146"
/note="Similar to YMR145C 'Mitochondrial external NADH
dehydrogenase, a type II NAD(P)H:quinone oxidoreductase
that catalyzes the oxidation of cytosolic NADH; Nde1p and
Nde2p provide cytosolic NADH to the mitochondrial
respiratory chain'"
/codon_start=1
/product="Nde1p"
/protein_id="EWG89421.1"
/translation="MIRQSLMKTVWANSSRFSLQSKSGLVKYAKNRSFHAARNLLEDK
KVILQKVAPTTGVVAKQSFFKRTGKFTLKALLYSALAGTAYVSYSLYREANPSTQVPQ
SDTFPNGSKRKTLVILGSGWGSVSLLKNLDTTLYNVVVVSPRNYFLFTPLLPSTPVGT
IELKSIVEPVRTIARRSHGEVHYYEAEAYDVDPENKTIKVKSSAKNNDYDLDLKYDYL
VVGVGAQPNTFGTPGVYEYSSFLKEISDAQEIRLKIMSSIEKAASLSPKDPERARLLS
FVVVGGGPTGVEFAAELRDYVDQDLRKWMPELSKEIKVTLVEALPNILNMFDKYLVDY
AQDLFKEEKIDLRLKTMVKKVDATTITAKTGDGDIENIPYGVLVWATGNAPREVSKNL
MTKLEEQDSRRGLLIDNKLQLLGAKGSIFAIGDCTFHPGLFPTAQVAHQEGEYLAQYF
KKAYKIDQLNWKMTHAKDDSEVARLKNQIVKTQSQIEDFKYNHKGALAYIGSDKAIAD
LAVGEAKYRLAGSFTFLFWKSAYLAMCLSFRNRVLVAMDWAKVIFWVEIHLSSRNRYK
RITFLNDDQFCFLFYFFPLVCFVFHIISFFIDILAEASSLFYANKIKIVLYT"
gene complement(525216..526259)
/gene="Tif34"
/locus_tag="P301_M12161"
mRNA complement(525216..526259)
/gene="Tif34"
/locus_tag="P301_M12161"
/product="Tif34p"
CDS complement(525216..526259)
/gene="Tif34"
/locus_tag="P301_M12161"
/note="Similar to YMR146C 'eIF3i subunit of the core
complex of translation initiation factor 3 (eIF3), which
is essential for translation; stimulates rate of ribosomal
scanning during translation reinitiation'"
/codon_start=1
/product="Tif34p"
/protein_id="EWG89422.1"
/translation="MKAIKLTGHERPLTQVKYNKEGDLLFSCSKDSSASVWYSLNGER
LGTLDGHTGTIWSIDVDCFTKYCVTGSADYSIKLWDVSNGQCVATWKSPVPVKRVEFS
PCGNYFLAILDNVMKNPGSINIYEIERDSATHELTKVSEEPIHKIITHEGLDAATVAG
WSTKGKYIIAGHKDGKISKYDVSNNYEYVDSIDLHEKSISDMQFSPDLTYFITSSRDT
NSFLVDVSTLQVLKKYETDCPLNTAVITPLKEFIILGGGQEAKDVTTTSANEGKFEAR
FYHKIFEEEIGRVQGHFGPLNTVAISPQGTSYASGGEDGFIRLHHFEKSYFDFKYDVE
KAAEAKEHMQEAN"
gene 526927..527598
/locus_tag="P301_M12166"
mRNA 526927..527598
/locus_tag="P301_M12166"
/product="hypothetical protein"
CDS 526927..527598
/locus_tag="P301_M12166"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89423.1"
/translation="MAARNRRKNNKKKSLLVTSAAQEKNATYVLVAEELHKNTIDLNM
GTETPLTENHENPIPAKEFKHQQKLEPIDEHDDGEDELSIKFKSMTKSSGPITEAEVQ
KLLLSYAFTSAAIQEDENEKESRHYPIKPPSPSASSLSAYFQSFVEKCKQVFYNFSLQ
TVEKLNALQNSLYEVFWIIFIYLNYWFPNVGDYVRYVCRSFSRHNEIAQLLTRIFTYN
INHLH"
gene 528097..528543
/locus_tag="P301_M12171"
mRNA 528097..528543
/locus_tag="P301_M12171"
/product="hypothetical protein"
CDS 528097..528543
/locus_tag="P301_M12171"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89424.1"
/translation="MVSTATFFFFVYLTLFVVIGFFSSLFIIPLLGISFVFAIGVVSF
GFCSNMSFKMAQLIYVRADAFLKKVLDKMALQTQPAQLQEPQEPLSTLRPVSNPTIPS
PLRQTARPSKFVTEEDVIFEPVSAQSAIARSLETAANKAGNKFQLS"
gene 528727..529587
/gene="Swp1"
/locus_tag="P301_M12176"
mRNA 528727..529587
/gene="Swp1"
/locus_tag="P301_M12176"
/product="Swp1p"
CDS 528727..529587
/gene="Swp1"
/locus_tag="P301_M12176"
/note="Similar to YMR149W Delta subunit of the
oligosaccharyl transferase glycoprotein complex, which is
required for N-linked glycosylation of proteins in the
endoplasmic reticulum"
/codon_start=1
/product="Swp1p"
/protein_id="EWG89425.1"
/translation="MQFFKTLATLVSCISFVLAYVAQDVHVSFPSTAGKSRVMIGKVE
PTIGIDETVPTTITVEDPNEVIQVNFAIESTNKPFQNTLLIGLPNKNLEMAFEPEIKD
NGKLSMYKYRIDLAKLDAALLQEASRSPELIKATLILASSTAKPKENLFREILQLNLN
FDVDHSDSSLVGKFGIKPEIHHIFHAKPKRVAKPIAVIFVLIIFITILSLIVTWLNSC
AAAFNNIPTGVTAVYFLGFIATIVGFEVIFARYYLGTSIFETLFSSLYLGAPGLLTST
KFLRSFGQTI"
gene complement(529682..530254)
/gene="Imp1"
/locus_tag="P301_M12181"
mRNA complement(529682..530254)
/gene="Imp1"
/locus_tag="P301_M12181"
/product="Imp1p"
CDS complement(529682..530254)
/gene="Imp1"
/locus_tag="P301_M12181"
/note="Similar to YMR150C 'Catalytic subunit of the
mitochondrial inner membrane peptidase complex, required
for maturation of mitochondrial proteins of the
intermembrane space; complex contains Imp1p and Imp2p
(both catalytic subunits), and Som1p'"
/codon_start=1
/product="Imp1p"
/protein_id="EWG89426.1"
/translation="MTVGTLPIWSKTFSYAIRSLCFLHIIHMYAYEFTETRGESMLPT
LSATNDYVHVLKNFQNGRGIKMGDCIVALKPTDPNHRICKRVTGMPGDLVLVDPSTIV
NYVGDVLVDEERFGTYIKVPEGHVWVTGDNLSHSLDSRTYNALPMGLIMGKIVAANNF
DKPFWDGSIRNIWGFKWINNTFLDVQAKSN"
gene 530822..531919
/gene="Yim1"
/locus_tag="P301_M12191"
mRNA 530822..531919
/gene="Yim1"
/locus_tag="P301_M12191"
/product="Yim1p"
CDS 530822..531919
/gene="Yim1"
/locus_tag="P301_M12191"
/note="Similar to YMR152W 'Protein of unknown function;
null mutant displays sensitivity to DNA damaging agents;
the authentic, non-tagged protein is detected in highly
purified mitochondria in high-throughput studies'"
/codon_start=1
/product="Yim1p"
/protein_id="EWG89427.1"
/translation="MSDEIVTNKSVTYVNNTTPVTITSSELDLRSCYQDDEVVIEVHA
AALNPIDFITHQLCNSYIFGKYPKTYSRDYSGVIIKAGKDVDNRWKVGDKVNGMYSHI
YGERGTLTHYLILNPAKDIPITHMVEVPKDENDPYDDFVYAAAWPLTFGTAFSTLYDF
KKDWTSDSKVLVIGASTSVSYAFVHIAKNYFNIGTVVGICSKNSIERNKKLGYDYLVP
YDEGSIVENVKKLKQIVLENDKFDMIFDSVGNHDFFPVIDQFLKPKAKNSFYVTIAGN
NKANYKNISWRDFVSLSSILKAINPFKKYNWRFGHPYPPNNFIEVGNEMIKKGTYKPP
IDSVYEFDQYKEAIDRLMSNRAKGKVVVKMK"
gene 532161..533588
/gene="Nup53"
/locus_tag="P301_M12196"
mRNA 532161..533588
/gene="Nup53"
/locus_tag="P301_M12196"
/product="Nup53p"
CDS 532161..533588
/gene="Nup53"
/locus_tag="P301_M12196"
/note="Similar to YMR153W Subunit of the nuclear pore
complex (NPC), interacts with karyopherin Kap121p or with
Nup170p via overlapping regions of Nup53p, involved in
activation of the spindle checkpoint mediated by the
Mad1p-Mad2p complex"
/codon_start=1
/product="Nup53p"
/protein_id="EWG89428.1"
/translation="MADLQKQENSSRFTNVSVIAPESQGQHEQQKQQEQQEQQKQPTG
LLKGLNGFPSAPQPLFMEDPPSTVSGELNDNPAWFNNPRKRAIPNSIIKRSNGQSLSP
VRSDSADVPAFSNSNGFNNVTFGSKKDPRILKNVSPNDNNSANNNAHSSDLGTVVFDS
NEAPPKTSLADWQKEDGIFSSKTDNIEDPNLSSNITFDGKPTATPSPFRPLEKTSRIL
NFFDKNTKTTPNTASSEASAGSKEGASTNWDDHAIIIFGYPETIANSIIFHFANFGEI
LEDFRVIKDFKKLNSKNKSKSPSLTAQKYPIYTGDGWVKLTYKSELSKSRALQENGII
MNGTLIGCVSYSPAALKQLASLKKSEEIINNKTSSQTSLSSKDLSNYRKTEGIFEKAK
AKAVTSKVRNAEFKVSKNSTSFKNPRRLEIKDGRSLFLRNRGKIHSGVLSSIESDLKK
REQASKSKKSWLNRLNNWLFGWNDL"
gene complement(533300..533635)
/locus_tag="P301_M12201"
mRNA complement(533300..533635)
/locus_tag="P301_M12201"
/product="hypothetical protein"
CDS complement(533300..533635)
/locus_tag="P301_M12201"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89429.1"
/translation="MLESHQSTTFGGKVFHYKSFHPNNQLFNLFNQLFLLLLACSLFF
KSDSIELRTPLWIFPLFLKNNERPSFISSLRGFLKEVEFLETLNSALRTFEVTAFALA
FSKIPSVFL"
gene complement(533725..535908)
/gene="Rim13"
/locus_tag="P301_M12206"
mRNA complement(533725..535908)
/gene="Rim13"
/locus_tag="P301_M12206"
/product="Rim13p"
CDS complement(533725..535908)
/gene="Rim13"
/locus_tag="P301_M12206"
/note="Similar to YMR154C 'Calpain-like cysteine protease
involved in proteolytic activation of Rim101p in response
to alkaline pH; has similarity to A. nidulans palB'"
/codon_start=1
/product="Rim13p"
/protein_id="EWG89430.1"
/translation="MNEWHEFNAAIKSIYCNAEGDSSSIINRLVGLAMKSDDSTFIQA
VLVLKENVSKVDKQLRFLWLTSTIDSRFYPPIPISEASPVSWNKTEYCAPGTEELQRR
YPGRAKLQNEEDYSGGIEQCRDVPDCSLVASLINLRSKNLNLPLIKQISSTKYHVNLS
FNGSNKRLVTVDISQIPTSVDGKQLSLKSNDISDKIGELALLLVSKGTYSTDGSNISI
DTYRLSGFLPEITQVNSYPFEKLWKFHKSNLCLMGAGTGNRSNDMIKPLVENHDYSII
DITYDSRLVKLRDPRNSALNVEISYEQYLKNFKQLYLNWNQEKLFKRSQVLHFRYDTS
RYNKFSIVADKPLFHLVNNSKVTETVWLLLESHLQNEGSQENRSVSFLNEAPECIICP
IEPPVECGGNRIGLQLVKLRLDAETERLLYCYSTTNNNFSIHSFSVVKEICFQRLKDT
KSLFAKVLFSFPYEIEGKASFDTCNFFQNPTFELEVHSEQDYQVLMDAACISTSSHDL
INIQVYYFNDYELIKPIMFDNHYQPGQGLKQDVPILTNVKYMIVCSTYGPPASMEFEL
LASIRLSSSWRLISGITLRSVNLIYGTYPYHCRNRFHWKETSDKLKIQMTLPTKKYST
NKLFIRVVPVESSARLRIRCNIFEPESALCVYECQEYRTCPSGGIVIPDLEVSRINIV
VLMIERSVPISSCLPTEGQLDELELFVGSSQKIRIEKYSDDVIPK"
gene 536277..537921
/locus_tag="P301_M12211"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 537035..537050
/estimated_length=16
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(538027..538746)
/gene="Tpp1"
/locus_tag="P301_M12216"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(539000..539767)
/gene="Aim36"
/locus_tag="P301_M12226"
mRNA complement(539000..539767)
/gene="Aim36"
/locus_tag="P301_M12226"
/product="Aim36p"
CDS complement(539000..539767)
/gene="Aim36"
/locus_tag="P301_M12226"
/note="Similar to YMR157C 'Protein of unknown function;
null mutant displays reduced respiratory growth and
elevated frequency of mitochondrial genome loss; the
authentic, non-tagged protein is detected in purified
mitochondria in high-throughput studies'"
/codon_start=1
/product="Aim36p"
/protein_id="EWG89431.1"
/translation="MLRPLRKSVLASCRHCFKVCGGLPQKQLPLFSPLLLRARYSSTD
SSTKRSNKSDKIDAPGFKKIFLVAIIGTVIFVKTVQSLDKNKPKTTLSEEEFENVVKG
LKRRVAIFPQGEVDIKFSLSPSIEETRKVLQKSQGDDINELQFVDPVKVIDYYRTLRD
DRYEALLNEYYKKYGCDTYAYNLPTGMLVMLLGRYFKENFKAGDKLVVVNFPHSIADA
TRFENEVSIVSKIFVPRKLSGSDVCKYYETVGKADII"
gene 539971..540438
/gene="Mrps8"
/locus_tag="P301_M12231"
mRNA 539971..540438
/gene="Mrps8"
/locus_tag="P301_M12231"
/product="Mrps8p"
CDS 539971..540438
/gene="Mrps8"
/locus_tag="P301_M12231"
/note="Similar to YMR158W Mitochondrial ribosomal protein
of the small subunit"
/codon_start=1
/product="Mrps8p"
/protein_id="EWG89432.1"
/translation="MSLVKLANTCAHLQNCSKVRVALTSIPYTKLQLQFAYNLYQQGF
LSSLQKGSTMGPDKDFVEVTPDNISTRRLWVGLKYRDNKPVLSSCKLISKPNSRIHLP
MEDMKKLCSGVTIRNIKPLQPGELILVRAHNNIMDINEAISKKLDGEVLCRVK"
gene complement(540609..540681)
/locus_tag="P301_M12236"
tRNA complement(540609..540681)
/locus_tag="P301_M12236"
/product="tRNA-Met"
/note="Elongator tRNA-Met;
TM(CAU)M"
assembly_gap 540627..540676
/estimated_length=50
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(540920..541057)
/locus_tag="P301_M12241"
mRNA complement(540920..541057)
/locus_tag="P301_M12241"
/product="hypothetical protein"
CDS complement(540920..541057)
/locus_tag="P301_M12241"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89433.1"
/translation="MKMNYHLSTSSYTTSMLSCTVLDDDIRYEKLSWKLDEAEMQGLI
M"
gene 542197..542517
/locus_tag="P301_M12246"
mRNA 542197..542517
/locus_tag="P301_M12246"
/product="hypothetical protein"
CDS 542197..542517
/locus_tag="P301_M12246"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89434.1"
/translation="MHHFVPSISLFMASVSFSVFFSHLATSWECSFFRSESFCCKTLF
SMVPLINSSFNLSVFLFFNAITSFNLRISCSLLFSSFCSIASVFNKASSWLTMLPPMA
PLLS"
gene complement(542202..542654)
/gene="Atg16"
/locus_tag="P301_M12251"
mRNA complement(542202..542654)
/gene="Atg16"
/locus_tag="P301_M12251"
/product="Atg16p"
CDS complement(542202..542654)
/gene="Atg16"
/locus_tag="P301_M12251"
/note="Similar to YMR159C Conserved protein that interacts
with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p
multimers, which localize to the pre-autophagosomal
structure and are required for autophagy"
/codon_start=1
/product="Atg16p"
/protein_id="EWG89435.1"
/translation="MGNFIITERKKAKEERSNPQTDSMDDLLIRRLTDRNDKEAHLNE
LFQDNSGAIGGNIVSHDDALLNTLAILQKELKSKEQEIRRLKEVIALKNKNTERLNDE
LISGTIENNVLQQKLSDLKKEHSQLVARWLKKTEKETEAMNSEIDGTK"
gene 542792..545242
/locus_tag="P301_M12256"
mRNA 542792..545242
/locus_tag="P301_M12256"
/product="hypothetical protein"
CDS 542792..545242
/locus_tag="P301_M12256"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89436.1"
/translation="MDVVQQKQDKQLQHQTQEQQHIREDQQEVPPQRPRQQNRWKPWW
NSTADDEPNTGRIAEYPNGQGRSSPTTDFQDSVNSNNDNKGIWSKIASFATSRYRSAP
IVVDDNTRYSQLNTEQINFLENEAKDIISKKSKSWCWYEAIPHISNSSNIIDSIDTPG
IISVSGTGSAKCPLPLNKYPGEGGNPGYNVFINDSLILPSDNPLNFLHVQPLRTKVLN
TIKNYYNFPNEQHLYLRQKKTALLKDKRIIIISVVGDLPEKYEQRSLESQRSAYYLSR
KLSQNLAQEQPQRVLTLSFQCPLHNQDLIPTYKECVELLNHWAHLFKEVDSIFFVGVY
HSVPLTLLLAKYIVQNNEVLEFDENTTVSVLSFQSCLQGYRFWDHSTDFTSNSYNNLG
SNSSTNENDSNDHDSNNDFTTKSQQIKEKQLFQGIDKKQQDTLSKIKNYRRIDSSESK
LVQDALDWLLFNWDTFRLTFFGKLYDNFMTISEKLAIDYNHPKILRNLWCNGKYMGID
LKNANNLNLDTDDEATSNINDVHVRTPNFESRLKIPTNRLFEITLWDILMITQNLGYK
QFIPIINLLSPFFISRSFNDYTLPPNIRKQYQNSNKIWLQEMDSKWKMNGHQLNYDQR
EGESLGSSSESLLPENISTVKDFLQFVQYQNEKSSDFVRIYSDIYDDDKVYKCFLYNT
IFTKNPLSRKHLRLNIDLDTPTSILNTVNQYDLVWKIHDSFSKLIQLKNLPQREIPHA
LRLSISLNCFLDSTTSTSGPVFQRDTVEALRRLTEIWRTYQDWSPPTRGLKHLRDILS
VLAMYDNPKNLINDVRRT"
gene 545443..546117
/gene="Hlj1"
/locus_tag="P301_M12261"
mRNA 545443..546117
/gene="Hlj1"
/locus_tag="P301_M12261"
/product="Hlj1p"
CDS 545443..546117
/gene="Hlj1"
/locus_tag="P301_M12261"
/note="Similar to YMR161W 'Co-chaperone for Hsp40p,
anchored in the ER membrane; with its homolog Ydj1p
promotes ER-associated protein degradation (ERAD) of
integral membrane substrates; similar to E. coli DnaJ'"
/codon_start=1
/product="Hlj1p"
/protein_id="EWG89437.1"
/translation="MSFTEDQEKIALEILSKDKHEFYEILKVDRKATDSEIKKAYRKL
AIKLHPDKNSHPKAGEAFKVINRAFEVLSNEEKRSIYDRIGRDPDDRQMPSRGAASGF
RGSAGGSPMGGGFEDMFFNSRFGGQRAGPPEDIFDFLFNAGGSPFGASPFGPSASTFS
FGGPGGFRVYTNNRGGSPFMRQQPRSRQQQQQAEENAVNSQLKNMLVLFIIFIVLPMI
KDYLFS"
gene complement(546676..551646)
/gene="Dnf3"
/locus_tag="P301_M12266"
mRNA complement(546676..551646)
/gene="Dnf3"
/locus_tag="P301_M12266"
/product="Dnf3p"
CDS complement(546676..551646)
/gene="Dnf3"
/locus_tag="P301_M12266"
/note="Similar to YMR162C 'Aminophospholipid translocase
(flippase) that maintains membrane lipid asymmetry in
post-Golgi secretory vesicles; localizes to the
trans-Golgi network; likely involved in protein transport;
type 4 P-type ATPase'"
/codon_start=1
/product="Dnf3p"
/protein_id="EWG89438.1"
/translation="MGIADGQRRRSSSLRTQMFNKHLYDKYRGRADDEIELEDINESK
TFSGSDNNDKDDRDETSGNYAAEEDYEMEEYGSPDVSYSIITKILDTILDRRRTFHSK
DGRHIPIILDHNAIEYKQAATKRDGHLIDERFNKPYCDNRITSSRYTFYSFLPRQLYA
QFSKLANTYFFIVAVLQMIPGWSTTGTYTTIIPLCVFMGISMTREAWDDFRRHRLDKE
ENNKPVGVLVKDGNNDAQEVYTLPSSVVSSTAYLTKSAAAENNPPLNDDRNSSQGHFL
DTHFNNFELLKNKYNVHIHQKKWEKLRVGDFVLLTQDDWVPADLLLLTCDGENSECFV
ETMALDGETNLKSKQPHPELNKLTKAASGLANINAQVTVEDPNIDLYNFEGNLELKNH
RNDTIMKYPLGPDNVIYRGSILRNTQNVVGMVIFSGEETKIRMNALKNPRTKAPKLQR
KINMIIVFMVFVVATISLFSYLGHVLHKKKYIDQNKAWYLFQADAGVAPTIMSFIIMY
NTVIPLSLYVTMEIIKVVQSKMMEWDIDMYHAETNTPCESRTATILEELGQVSYIFSD
KTGTLTDNKMIFRKFSLCGSSWLHNVDLGNSEDNFEDNRDNTNSLRLPPKAHNGSSID
VVSIGDQNVLDRLGFSDAPIEKGHRPSLDNFPKSRNSIEYKGNSSAIYTGRPSMRSLF
GKDNSHLSKQASVISPSETFSENIKSSFDLIQFIQRYPTALFSQKAKFFFLSLALCHS
CLPKKTHNESIGEDSIEYQSSSPDELALVTAARDLGYIVLNRNAQILTIKTFPDGFDG
EAKLENYEILNYIDFNSQRKRMSVLVRMPTQPNQVLLICKGADNVIMERLHDRELAAK
KMADICTSTKERKDAEAELVLQQRKSLERMVDEEAMARTSLRNSLSSVPRASLSLQAV
RKSLSMKNSRTRDPEKQIDSIDQFLETVKKSDQEIGSVVNKSRKSLHKQQIEKYGPRI
SIDGTHFPNNNVPIDTRKEGLQHDYDTEILEHIGSDELILNEEYVIERTLQAIDEFST
EGLRTLVYAYKWIDIGQYENWNKRYHQAKTSLTDRKIKVDEAGAEIEDGLNLLGVTAI
EDKLQDGVSEAIEKIRRAGIKMWMLTGDKRETAINIGYSCMLIKDYSTVVILTTTDEN
IISKMNAVSQEVDSGNIAHCVVVIDGATMAMFEGNPTYMSVFVELCTKTDSVICCRAS
PSQKALMVSNIRNTDPNLVTLAIGDGANDIAMIQSADIGVGIAGKEGLQASRVSDYSI
GQFRFLLKLLFVHGRYNYIRTSKFMLCTFYKEITFYFTQLIYQRYTMFSGSSLYEPWS
LSMFNTLFTSLPVLCIGMFEKDLKPMTLLTVPELYSYGRLSQGFNWLIFMEWVILATT
NSLIITFLNVVMWGMSSLSDNTMYPLGLINFTAIVALINVKSQFVEMHNRNWLAFTSV
VLSCGGWLVWCCALPILNNTDQIYDVAYGFYNHFGKDITFWCTSLVLALLPITLDIVY
KTFKVMIWPSDSDIFAELEQKSDIRKKLELGAYSEMRQGWTWDKDPSTFTRYTDKVLS
RPRTNSRASAKTHNSSIYSMSNGNVDHSSKKNFFGSSSKKSSERYEVLPSGKLIKRPS
LKTQSSKDSIGGNITTKLTKKLKLPSRNVEDEDVNQIIQARLKDLE"
gene complement(551994..554111)
/gene="Inp2"
/locus_tag="P301_M12271"
mRNA complement(551994..554111)
/gene="Inp2"
/locus_tag="P301_M12271"
/product="Inp2p"
CDS complement(551994..554111)
/gene="Inp2"
/locus_tag="P301_M12271"
/note="Similar to YMR163C 'Peroxisome-specific receptor
important for peroxisome inheritance; co-fractionates with
peroxisome membranes and co-localizes with peroxisomes in
vivo; physically interacts with the myosin V motor Myo2p;
INP2 is not an essential gene'"
/codon_start=1
/product="Inp2p"
/protein_id="EWG89439.1"
/translation="MTTNSRPSALQAPGLQIFSMLKSSEEDGFMSSSLTLDSDNIIGV
TENNRQEFYSTWRKPSLLSSRSVLHEYSPTIVGSNDSTFSPITVGKTTKFFNWDDIIS
RIFMQQPFGVTHQFFEEFRYSIITSHFLNDMNHYRLSLHLDQSIMNFHKSSTLLKNVP
PKSVPFMATKYGKLAVVEDKKLYFRQNFNYLSMIITSYRVLTQLKKYCRKKNSPGLKR
VVILILVAVYLSIQQEYFRRHLICYKTLLKVRKVLESLQQVDVMIHKYHLRFKEIKNH
SFISRVSLISIADEHSSVIKELLVFSSDALFYKLKSIIPDIVIFSDTSELSKYCELYG
IDVPNLYYNNTTTVKDLDGKLYRLKLLKKFMLCCLLSLDMTGNENLSNVNMRNALNKI
FPDYMARVQLKKKYNPIGTFQNIVSLLRGLHSLLSTVLVSLNDHKQILYAFPEETSTN
TGCERANVCSFSKNDKLFQALNYLKMIENNLLAIDIRNGITENDRNIIEDKLEELITF
WKTSKICGNISRIQKVSPTNTINHGFHLDILKGRKSPRSSSVQGLSLERKVDFIDVAE
SVNDSFENDTELEEYEDYDCQEGCSAGSRQNHRVDFIGKDNCRKPDFKQLSDNELRRK
LDERILKLAQENREGRERLRTAKSFELLRKAQASMSVKFGFQKPLRDDAFLESRPLSK
CKVSSEETIPFLYELKGLLGNHS"
gene complement(554359..554432)
/locus_tag="P301_M12276"
tRNA complement(554359..554432)
/locus_tag="P301_M12276"
/product="tRNA-Val"
/note="TV(AAC)M3"
gene complement(554996..557248)
/gene="Mss11"
/locus_tag="P301_M12281"
mRNA complement(554996..557248)
/gene="Mss11"
/locus_tag="P301_M12281"
/product="Mss11p"
CDS complement(554996..557248)
/gene="Mss11"
/locus_tag="P301_M12281"
/note="Similar to YMR164C 'Transcription factor involved
in regulation of invasive growth and starch degradation;
controls the activation of MUC1 and STA2 in response to
nutritional signals'"
/codon_start=1
/product="Mss11p"
/protein_id="EWG89440.1"
/translation="MDNTTNINTNERSSNTDFSSAPNIKGLNSHTQLQFDADSRVFVS
DAMAKNSKQLLYAHIYNYLSKNNYWNSAAKFLSEADLPLCRINGSASGEKTSLNASLK
QGLMDIASKGDIVSEDGLLPSKMLMDANDTFLLEWWEIFQSLFNGDLESGYQQDHNPL
RERIIPILPANSKSNMPSHFSNLPPNVIPPTQNSFPVSEESFRPNGDGSNFNLNDPTN
RNVSERFLSRTSGVYDKQNSANFAPDTAINSDIAGQQYATINLHKHFNDLQSPAQPQQ
SSQQQIQQPQHQPQHQPQQQPQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTPYPIVN
PQMVPHIPSENSHSTGLMPSVPPTNQQFNAQTQSSMFSDQQRFFQYQLHHQNQGQAPS
FQQSQSGRFDDMNAMKMFFQQQALQQNSLQQNLGNQNYQSNTRNNTAEETTPTNDNNA
NGNSLLQEHIRARFNKMKTIPQQMKNQNTVANPVVSDITSQQQYMHMMMQRMAANQQL
QNSAFPPDTNRIAPANNAMPLQPGNMGPPVIENPGMRQTNPSGQNPMINMQPLYQNVS
SAMHAFAPQQQFHLPQHYKTNTSVPQNDSTSVFPLPNNNNNNNNNNNNNNNSNNSNNN
NNNDNSNNTPTVSQPSSKRTSSSSTTPNITTTIQPKRKQRVGKTKTKESRKVAAAQKV
MKSKKLEQNGDSAATNFINVTPKDSGGKGTVKVQNSNSQQQLNGSFSMDTETFDIFNI
GDFSPDLMDS"
gene complement(557737..560325)
/gene="Pah1"
/locus_tag="P301_M12286"
mRNA complement(557737..560325)
/gene="Pah1"
/locus_tag="P301_M12286"
/product="Pah1p"
CDS complement(557737..560325)
/gene="Pah1"
/locus_tag="P301_M12286"
/note="Similar to YMR165C 'Mg<sup>2+</sup>-dependent
phosphatidate (PA) phosphatase, catalyzes the
dephosphorylation of PA to yield diacylglycerol,
responsible for de novo lipid synthesis and formation of
lipid droplets; homologous to mammalian lipin 1'"
/codon_start=1
/product="Pah1p"
/protein_id="EWG89441.1"
/translation="MQYVGRALGSVSKTWSSINPATLSGAIDVIVVEHPDGRLSCSPF
HVRFGKFQILKPSQKKVQVFINEKLSNMPMKLSDSGEAYFVFEMGDQVTDVPDELLVS
PVMSATSSPPQSPETSILEGGTEGEGEGENENKKKEKKVLEEPDFLDINDTGDSGSKN
SETTGSLSPTESSTTTPLDSVEERKLVEQRTKNFQQKLNKKLTEIHIPSKLDNNGDLL
LDTEGYKPNKNMMHDTDIQLKQLLKDEFGNDSDISSFIKEDKNGNIKIVNPYEHLTDL
SPPGTPPTMATSGSVLGLDAMESGSTLNSLSSSPSGSDTEDETSFSKEQSSKSEKTSK
KGTAGSGETEKRYIRTIRLTNDQLKCLNLTYGENDLKFSVDHGKAIVTSKLFVWRWDV
PIVISDIDGTITKSDALGHVLAMIGKDWTHLGVAKLFSEISRNGYNILYLTARSAGQA
DSTRSYLRSIEQNGSKLPNGPVILSPDRTMAALRREVILKKPEVFKIACLNDIRSLYF
EDSDNEMDTEEKSTPFFAGFGNRITDALSYRTVGIPSSRIFTINTEGEVHMELLELAG
YRSSYIHINELVDHFFPPVSLDSVDLRTNTSMVPGSPPNRTLDNFDSEITSGRKTLFR
GNQEEKFTDVNFWRDPLVDIDNLSDISNDDSDNIDEDTDVSQQSNVSRNRANSVKTAK
VTKAPQRNVSGSTNNNEVLAASSDVENASDLVGSHSSSGSTPNKSTMSKGDIGKQIYL
ELGSPLASPKLRYLDDMDDEDSNYNRTKSRRASSAAATSIDKEFKKLSVSKAGAPTRI
VSKIDVSNDVHSLGNSDTESRREQSVNETGRNQLPHNSMDDKDLDSRVSDEFDDDEFD
EDEFED"
gene complement(561065..562171)
/locus_tag="P301_M12291"
mRNA complement(561065..562171)
/locus_tag="P301_M12291"
/product="hypothetical protein"
CDS complement(561065..562171)
/locus_tag="P301_M12291"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89442.1"
/translation="MNSWNLSSSIPIIHTPHDHPPTSEGTPDQPNNNRKDDKLHKKRG
DSDEDLSPIWHCVVSGGIGGIIGDSAMHSLDTVKTRQQGAPNVKKYRNMISAYRTIWL
EEGVRRGLYGGYMAAMLGSFPSAAIFFGTYEYTKRTMIEDWQINDTITHLSAGFLGDF
ISSFVYVPSEVLKTRLQLQGRFNNPFFQSGYNYSNLRNAIKTVIKEEGFRSLFFGYKA
TLARDLPFSALQFAFYEKFRQLAFKIEQKDGRDGELSIPNEILTGACAGGLAGIITTP
MDVVKTRVQTQQPPSQSNKSYSVTHPHVTNGRPAALSNSISLSLRTVYQSEGVLGFFS
GVGPRFVWTSVQSSIMLLLYQMTLRGLSNAFPTD"
gene 562584..564893
/gene="Mlh1"
/locus_tag="P301_M12296"
mRNA 562584..564893
/gene="Mlh1"
/locus_tag="P301_M12296"
/product="Mlh1p"
CDS 562584..564893
/gene="Mlh1"
/locus_tag="P301_M12296"
/note="Similar to YMR167W 'Protein required for mismatch
repair in mitosis and meiosis as well as crossing over
during meiosis; forms a complex with Pms1p and Msh2p-Msh3p
during mismatch repair; human homolog is associated with
hereditary non-polyposis colon cancer'"
/codon_start=1
/product="Mlh1p"
/protein_id="EWG89443.1"
/translation="MSLRIKALDASVVNKIAAGEIIISPVNALKEMMENSIDANATMI
DILVKEGGIKVLQITDNGSGINKADLPILCERFTTSKLQKFEDLSQIQTYGFRGEALA
SISHVARVTVTTKVKEDRCAWRVSYAEGKMLESPKPVAGKDGTTILVEDLFFNIPSRL
RALRSHNDEYSKILDVVGRYAIHSKDIGFSCKKFGDSNYSLSVKPSYTVQDRIRTVFN
KSVASNLITFHISKVEDLNLESVDGKVCNLNFISKKSISPIFFINNRLVTCDPLRRAL
NSVYSNYLPKGNRPFIYLGIVIDPAAVDVNVHPTKREVRFLSQDEIIEKIANQLHAEL
SAIDTSRTFKASSISTNKPESLIPFNDTIESDRNRKSLRQAQVVENSYTTANSQLRKA
KRQENKLVRIDASQAKITSFLSSSQQFNFEGSSTKRQLSEPKVTNVSHSQEAEKLTLN
EGEQPRDANTINDNDLKDQPKKKQKLGDYKVPSIADDEKNALPISKDGYIRVPKERVN
VNLTSIKKLREKVDDSIHRELTDIFANLNYVGVVDEERRLAAIQHDLKLFLIDYGSVC
YELFYQIGLTDFANFGKINLQSTNVSDDIVLYNLLSEFDEFNDDASKEKIISKIWDMS
SMLNEYYSIELVNDGLDNDLKSVKLKSLPLLLKGYIPSLVKLPFFIYRLGKEVDWEDE
QECLDGILREIALLYIPDMVPKVDTSDASLSEDEKAQFINRKEHISSLLEHVLFPCIK
RRFLAPRHILKDVVEIANLPGLYKVFERC"
gene complement(565030..566856)
/gene="Cep3"
/locus_tag="P301_M12301"
mRNA complement(565030..566856)
/gene="Cep3"
/locus_tag="P301_M12301"
/product="Cep3p"
CDS complement(565030..566856)
/gene="Cep3"
/locus_tag="P301_M12301"
/note="Similar to YMR168C 'Essential kinetochore protein,
component of the CBF3 complex that binds the CDEIII region
of the centromere; contains an N-terminal Zn2Cys6 type
zinc finger domain, a C-terminal acidic domain, and a
putative coiled coil dimerization domain'"
/codon_start=1
/product="Cep3p"
/protein_id="EWG89444.1"
/translation="MFNRTTQLKSKHPCSVCTRRKVKCDRMIPCGNCRKRGQDSECMK
STKLITASSSKEYLPDLLLFWQNYEYWITNIGLYKTKQRDLTRTPANLDTDTEECMFW
MNYLQKDQSFQLMNFAMENLGALYFGSIGDISELYLRVEQYWDRRADKNHSVDGKYWD
ALIWSVFTMCIYYMPVEKLAEIFSVYPLHEYLGSNKRLNWEDGMQLVMCQNFARCSLF
QLKQCDFMAHPDIRLVQAYLILATTTFPYDEPLLANSLLTQCIHTFKNFHVDDFRPLL
NDDPVESIAKVTLGRIFYRLCGCDYLQSGPRKPIALHTEVSSLLQHAAYLQDLPNVDV
YREENSTEVLYWKIISLDRDLDQYLNKSSKPPLKTLDAIRRELDIFQYKVDSLEEDFR
SNNSRFQKFIALFQISTVSWKLFKMYLIYYDTADSLLKVIHYSKVIISLIVNNFHAKS
EFFNRHPMVMQTITRVVSFISFYQIFVESAAVKELLVDLTELTANLPTIFGSKLDKLG
YLTERLSKLKLLWDKVQLLDSGDSFYHPVFKILQNDIKIIELKNDEMFSLIKGLGSLV
PLNKLRQESLLEEEDENNTEPSDFRTIVEEFQSEYNISDILS"
gene complement(567050..568570)
/gene="Ald2"
/locus_tag="P301_M12321"
mRNA complement(567050..568570)
/gene="Ald2"
/locus_tag="P301_M12321"
/product="Ald2p"
CDS complement(567050..568570)
/gene="Ald2"
/locus_tag="P301_M12321"
/note="Similar to YMR170C 'Cytoplasmic aldehyde
dehydrogenase, involved in ethanol oxidation and
beta-alanine biosynthesis; uses NAD+ as the preferred
coenzyme; expression is stress induced and glucose
repressed; very similar to Ald3p'"
/codon_start=1
/product="Ald2p"
/protein_id="EWG89445.1"
/translation="MPTLYTDIEIPQLKISLKQPLGLFINNEFCPSSDGKTIETVNPA
TGEPITSFQAANEKDVDKAVKAARAAFDNVWSKTSSEQRGIYLSNLLKLIEEEQDTLA
TLETLDSGKPFHSNAKQDLAQIIELTRYYAGAVDKFNMGETIPLTFNKFAYTLKVPFG
VVAQIVPWNYPLAMACRKMQGALAAGNTVIIKPAENTSLSLLYFATLIKKAGFPPGVV
NVIPGYGSVVGKALGTHMDIDKISFTGSTKVGGSVLEASGQSNLKDITLECGGKSPAL
VFEDADLDKAIEWVANGIFFNSGQICTANSRVYVQSSIYDKFVEKFKETAKKEWDVAG
KFDPFDEKCIVGPVISSTQYDRIKSYIERGKKEEKLDMFQTSEFPVSGAKGYFIPPTI
FTDVPETSKLLRDEIFGPVVVVSKFTNYDDALKLANDTCYGLASAVFTKDVKKAHMFA
RDIKAGTVWINQTNQEEAKVPFGGFKMSGIGRESGDTGVDNYLQIKSVHVDLSLDK"
gene complement(569356..571008)
/gene="Ear1"
/locus_tag="P301_M12326"
mRNA complement(569356..571008)
/gene="Ear1"
/locus_tag="P301_M12326"
/product="Ear1p"
CDS complement(569356..571008)
/gene="Ear1"
/locus_tag="P301_M12326"
/note="Similar to YMR171C 'Specificity factor required for
Rsp5p-dependent ubiquitination and sorting of specific
cargo proteins at the multivesicular body; mRNA is
targeted to the bud via the mRNA transport system
involving She2p'"
/codon_start=1
/product="Ear1p"
/protein_id="EWG89446.1"
/translation="MSFKFLIESLLLGSISGQIRCGRSSVIPRGDVSYGGDDTDELNM
DIMLFAFGTLIVVYIVICIVYFFTKQIATRLITAYYNEHGPGQRISLFSDYDENNAHV
HSRRLMENMSLRWPNNLDDADEVRDKLAQLSPEEQFYYKQGEEYIKQNPPFLLNQELL
QQSEDSNPDTTREDPIMNEQTRQYIQEEGAYAWEFSPNPDMPNHTVIVENKTEVSFLN
YNYDASISTNLPIPCINKVYYCEFKIFETDGPLNSDENVSKGVISFGLSTQPYPYFRL
PGRHHHSIAYDSNGARRFNDSFKLNEQLRTLFPQCEKGDIVGIGYRSRSGTVFFTRNG
KKLNEKSVGGHIRGWKFQYLYPIIGSNVPCQIHVNFGTYGFVYIEANVKKWGYAKSNG
IKLPPPSYEDYGKDTLLESGGEDNDFDEDFSDGDSDNIAAGSTTNLNDDIIIRNGEIL
PPPPGFEFTMSPPTGKKIINEEINLDSLPMLPPSYSDDEHHSKNDKSAISGRIIGTSR
NLITDEASFDSVDNDNEDENDHERDPEQFSEFDDYESRMHGI"
gene 571467..573626
/gene="Hot1"
/locus_tag="P301_M12331"
mRNA 571467..573626
/gene="Hot1"
/locus_tag="P301_M12331"
/product="Hot1p"
CDS 571467..573626
/gene="Hot1"
/locus_tag="P301_M12331"
/note="Similar to YMR172W 'Transcription factor required
for the transient induction of glycerol biosynthetic genes
GPD1 and GPP2 in response to high osmolarity; targets
Hog1p to osmostress responsive promoters; has similarity
to Msn1p and Gcr1p'"
/codon_start=1
/product="Hot1p"
/protein_id="EWG89447.1"
/translation="MSGMGIAILCIVRTKIYRITISFDYSTLMSPFFLFLMMPTTLKD
GYRMNSQVNEDAIGINLDLSLPTHISPTTGSESASGSNASTLRNDGNALDGCLLRTSA
AISAPTGTSQPTETIGEKLSNEERVNSNVSASNSTTAGTGRMLSQSLTNDSPSNEIST
DQLKIFQRMDEMSARMIEMEESFNKLSNKIAEQNTMVLNLKQDNYKVMNKLNILLKLV
AQPSARPSTNNAQNKLAIELLNSISAVSSAYLQKMQNNGSGRQHTADLCTGDSNTHSG
INQHRTTNGTIDVNTNTAQLNNQFSNALNTILPDQQHNRNNVSQNINQSLPNRQLGPV
INTQANQNQSQVLIHNTNTHQQVNRSPISFPNASTDKPFKLNPNGIKRRRRNTQSNNN
ASTNDHASAAQKPISALSPLTNSHNSTTSMNYTNSSIHSGVTSASNSFHDLNSLNNFG
TTTALSLPSLALDNASFPPNQNVIPPIINNTQQPLSFSQLINQDSTTSELLPSGKSGV
NTNIVNRNRASTLPSYPKPMTVKSNVDDDGYQEDDDDDGDDEGDGRDNEEDSTAEEDE
VDDEIETDMKNASINKRRRSLHHKKSNSLNGRRKLHGESATKPNINSDLHYRILKAPT
DVKTIWEEYDTGIRGKPSIKHLEAKYGNKWRLNKNKKTFSRRKRLYKFILNGMERGKT
AQEMIETLENKRLYKDDEDGEVKKRTIGWLQESLAGI"
gene complement(573314..573697)
/locus_tag="P301_M12336"
mRNA complement(573314..573697)
/locus_tag="P301_M12336"
/product="hypothetical protein"
CDS complement(573314..573697)
/locus_tag="P301_M12336"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89448.1"
/translation="MYLCYTCFFLPSYDCKRLFTIVRAYIPARLSCNQPMVLFFTSPS
SSSLYSLLFSKVSIISCAVLPLSIPFRMNLYNLFRLEKVFLFLLSLHLLPYLASRCLI
DGFPLIPVSYSSHIVFTSVGAFNIL"
gene 574175..575577
/gene="Ddr48"
/locus_tag="P301_M12341"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 574384..575578
/locus_tag="P301_M12346"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 574520..575107
/estimated_length=588
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(575655..575861)
/gene="Pai3"
/locus_tag="P301_M12351"
mRNA complement(575655..575861)
/gene="Pai3"
/locus_tag="P301_M12351"
/product="Pai3p"
CDS complement(575655..575861)
/gene="Pai3"
/locus_tag="P301_M12351"
/note="Similar to YMR174C 'Cytoplasmic proteinase A
(Pep4p) inhibitor, dependent on Pbs2p and Hog1p protein
kinases for osmotic induction; intrinsically unstructured,
N-terminal half becomes ordered in the active site of
proteinase A upon contact'"
/codon_start=1
/product="Pai3p"
/protein_id="EWG89449.1"
/translation="MNTDQQKVSEIFQSSKEKLQGDAKVVSDAFKKMASQDKDGKTTD
ADESEKHNYQEQYNKLKGAGHKKE"
gene 576512..576751
/gene="Sip18"
/locus_tag="P301_M12356"
mRNA 576512..576751
/gene="Sip18"
/locus_tag="P301_M12356"
/product="Sip18p"
CDS 576512..576751
/gene="Sip18"
/locus_tag="P301_M12356"
/note="Similar to YMR175W 'Phospholipid-binding
hydrophilin with a role in dessication resistance;
expression is induced by osmotic stress'"
/codon_start=1
/product="Sip18p"
/protein_id="EWG89450.1"
/translation="MSNMMNKFAEKLQGNDDSHQKGKNAKSSNKERDDMNMDMGMGHD
QSEGGMKMGHDQSGTKMNAGRGIANDWKTYENMKK"
gene 576810..577008
/locus_tag="P301_M12361"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 577202..577212
/estimated_length=11
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 577240..581155
/gene="Ecm5"
/locus_tag="P301_M12371"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 582068..583600
/gene="Mmt1"
/locus_tag="P301_M12381"
mRNA 582068..583600
/gene="Mmt1"
/locus_tag="P301_M12381"
/product="Mmt1p"
CDS 582068..583600
/gene="Mmt1"
/locus_tag="P301_M12381"
/note="Similar to YMR177W 'Putative metal transporter
involved in mitochondrial iron accumulation; closely
related to Mmt2p'"
/codon_start=1
/product="Mmt1p"
/protein_id="EWG89451.1"
/translation="MLRICVKRPCIKIVLSQVRPALLVRKENLHISTGVKVEKSSIIN
QKDPNKVRVEINELKRQAEIEKAAIKELEKNPQYQKLAEAFNSHDHVHLRESETEQND
IISLGTIRDYKSSKREQADKPSSLNLHSHTHSHGHTHSHAAHNPLLVLSTEQIRKNAG
VRITWVGLGVNVGIAIGKFFGGIVFHSQALFADAIHAISDMVSDLLTLLSVGLAANKP
TADYPYGYGKIETVGSLAVSTILAMAGISIGWSSLCALVGPVIPHTIIDTIGNLGHAH
TYSEDIIEDVTDINAAWIAAASIAAKEWIFRATRKIAINTNSNVLMANAWHHRVDSLT
SLVALVAISTGYLVNIQSLDTIGGLIVSGLIIKAGGEGMCIAIKELIDQSVSRDDPRY
LEIETLVKDTLNKLISNNNSQKPYGLKELTLLSSGPNLRGHLTLEVPLQKWGNILGVN
EFEIVTHHLRNVLTNEVSNLRRLDIEYVEEKNGEENEHIKGQQNYKEDVLIKHDHTNT
HI"
assembly_gap 583619..583673
/estimated_length=55
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 583981..584618
/locus_tag="P301_M12391"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 585172..587447
/gene="Spt21"
/locus_tag="P301_M12401"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(587568..588530)
/gene="Ctl1"
/locus_tag="P301_M12411"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(588933..589397)
/locus_tag="P301_M12416"
mRNA complement(588933..589397)
/locus_tag="P301_M12416"
/product="hypothetical protein"
CDS complement(588933..589397)
/locus_tag="P301_M12416"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89452.1"
/translation="MTPLLQAEAKMNTSLYLTESIQQHEFNLTSPQSFYSSPSVPNSK
NNSGIFSYNTANNSRVSSSDEFTTQQDGMNTIMYKNNISKTFEDDIFYCPRSLLTPEE
QVVYQXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXKSFSPASSE"
assembly_gap 588965..589074
/estimated_length=110
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(589848..590483)
/gene="Rgm1"
/locus_tag="P301_M12431"
mRNA complement(589848..590483)
/gene="Rgm1"
/locus_tag="P301_M12431"
/product="Rgm1p"
CDS complement(589848..590483)
/gene="Rgm1"
/locus_tag="P301_M12431"
/note="Similar to YMR182C 'Putative transcriptional
repressor with proline-rich zinc fingers; overproduction
impairs cell growth'"
/codon_start=1
/product="Rgm1p"
/protein_id="EWG89453.1"
/translation="MRGKQPKRNKDNASVKRNYRCVGYPDCNMSFNRAEHLARHIRKH
TGEKPFQCNICLKFFSRIDNLRQHQSSVHSDVDLMSLRRLQQSANSTANDPNATRMFP
QLRPYGIVVQPAPVPYNLPISTPASPQNTISLYAPPYFPHPMPSAPIPLPHQPPPLPI
YSYMQPLFLNHTPIQNHNIVELPPDSSDTPASPSKVQSFDQAKDASPNAKK"
gene 591103..591189
/locus_tag="P301_M12441"
mRNA 591103..591189
/locus_tag="P301_M12441"
/product="hypothetical protein"
CDS 591103..591189
/locus_tag="P301_M12441"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89454.1"
/translation="MRKPSAFHACNIIFLPLVKCASATIMLN"
gene 591640..591945
/locus_tag="P301_M12446"
ncRNA 591640..591945
/ncRNA_class="snoRNA"
/locus_tag="P301_M12446"
/product="SNR83"
gene complement(592212..593099)
/gene="Sso2"
/locus_tag="P301_M12451"
mRNA complement(592212..593099)
/gene="Sso2"
/locus_tag="P301_M12451"
/product="Sso2p"
CDS complement(592212..593099)
/gene="Sso2"
/locus_tag="P301_M12451"
/note="Similar to YMR183C 'Plasma membrane t-SNARE
involved in fusion of secretory vesicles at the plasma
membrane; syntaxin homolog that is functionally redundant
with Sso1p'"
/codon_start=1
/product="Sso2p"
/protein_id="EWG89455.1"
/translation="MSNANPYENNNPYAENYEMQEDLNNAPTGHSDGSDDFVAFMNKI
NSINANLSRYENIINQIDAQHKDLLTQVSEEQEMELRRSLDDYISQATDLQYQLKADI
KDAQRDGLHDSNKQAQAENCRQKFLKLIQDYRIIDSNYKEESKEQAKRQYTIIQPEAT
DEEVEAAINDVNGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKTMAELTQLF
NDMEELVIEQQENVDVIDKNVEDAQQDVEQGVGHTNKAVKSARKARKNKIRCLIICFI
IFAIVVVVVVVPSVVETRK"
gene 593480..594076
/gene="Add37"
/locus_tag="P301_M12456"
mRNA 593480..594076
/gene="Add37"
/locus_tag="P301_M12456"
/product="Add37p"
CDS 593480..594076
/gene="Add37"
/locus_tag="P301_M12456"
/note="Similar to YMR184W 'Protein of unknown function
involved in ER-associated protein degradation; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm and is induced in response to the DNA-damaging
agent MMS; YMR184W is not an essential gene'"
/codon_start=1
/product="Add37p"
/protein_id="EWG89456.1"
/translation="MAIKPTKSFQNCLEAEVPGYNDCPTVLFSIDPNSGPRSKSKQRT
KSKRCVSGRLATEVLDLYGNTKTATTPPPVLKRPSVTAAQQESACEGVLVKDQGDRQL
QPILCSKEELVAKINDLCVCGSKLSSKELEFYKKKLDSNITKILQNEHTKTVLSQIFN
EKDKNMAVKTVKHWMVTDTTISNWCPAFLKIFENAMPN"
gene 594314..597165
/locus_tag="P301_M12466"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 596109..596154
/estimated_length=46
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 597642..599758
/gene="Hsc82"
/locus_tag="P301_M12471"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(599975..601270)
/locus_tag="P301_M12476"
mRNA complement(599975..601270)
/locus_tag="P301_M12476"
/product="hypothetical protein"
CDS complement(599975..601270)
/locus_tag="P301_M12476"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89457.1"
/translation="MTPPHFFLSLIKKRCICWICLEESTYDSTWLQHTCGCNLQIHKR
CYIRWLYQMHVELFLPNTVDLPKDADLPIITCLKCLVDGHHDFMTTFSLTEIWETRPI
WGQKSVPFQNDYVFNLMSLYTKRDNHPPYVLVKFGECPQCKNTNFIKRPTVTIQSSVL
SLFYQWQKITRYVIPLGITSLFLLNPEKTSFDIGLWQLRCLFPENVLRNMLNISTTKA
LDVYAQTERGLLSIPLTSSIIIYGFIHYLSNISNVSANAILFKWVYLSIVKTAGNKYY
KGIGLPKIILYSNLATFCYNFTFKRLVDLIYGRLINKGGKYLYHGNFENSSNSVPAEE
FFIRRNWYAILAEKILWPFVGKCTGGLLLNAFLWIQRKFKIEWTPNCSPSEFRMIFNI
IGCGTAAISWSSLKLYASYKRCQELEKINEFIEQSCKGE"
gene complement(601577..602291)
/gene="Mrps17"
/locus_tag="P301_M12481"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 602787..605891
/gene="Gcv2"
/locus_tag="P301_M12486"
mRNA 602787..605891
/gene="Gcv2"
/locus_tag="P301_M12486"
/product="Gcv2p"
CDS 602787..605891
/gene="Gcv2"
/locus_tag="P301_M12486"
/note="Similar to YMR189W 'P subunit of the mitochondrial
glycine decarboxylase complex, required for the catabolism
of glycine to 5,10-methylene-THF; expression is regulated
by levels of 5,10-methylene-THF in the cytoplasm'"
/codon_start=1
/product="Gcv2p"
/protein_id="EWG89458.1"
/translation="MLRTRVTALLCRATVRSSTNYVSLARTRSFHSQSILLKTAATDI
TSTQYSRIFNPDLKNIDRPLDTFARRHLGPSPSDVKKMLKTMGYSDLNAFIEELVPPN
ILKRRPLKLEAPSKGFCEQEMLQHLEKIANKNHYKVKNFIGKGYYGTILPPVIQRNLL
ESPEWYTSYTPYQPEISQGRLEALLNFQTVVSDLTGLPVANASLLDEGTAAGEAMLLS
FNISRKKKLKYVIDKKLHQQTKSVLHTRAKPFNIEIIEVDCSDIKKAVDVLKNPDVSG
CLVQYPATDGSILPPDSMKQLSDALHSHKSLLSVASDLMALTLLKPPAHYGADIVLGS
SQRFGVPMGYGGPHAAFFAVIDKLNRKIPGRIVGISKDRLGKTALRLALQTREQHIKR
DKATSNICTAQALLANVASSYCVYHGPKGLQNISRRIFSLTSILANAIENDSCPHELI
NKTWFDTLTIKLGNGISSEQLLDKALKEFNINLFAVDTTTISLALDETTTKADVENLL
KMFDIENSSQFLSEDYSNSFPREFQRTDEILRNEVFHMHHSETAMLRYLHRLQSRDLS
LANSMIPLGSCTMKLNSTVEMMPITWPQFSNIHPFQPSNQVQGYKELITSLEKDLCSI
TGFDGISLQPNSGAQGEYTGLRVIRSYLESKGENHRNVCLIPVSAHGTNPASAAMAGL
KVVPVNCLQDGSLDLVDLKNKAEQHSKELAAVMITYPSTYGLFEPGIQHAIDIVHSFG
GQVYLDGANMNAQVGLTSPGDLGADVCHLNLHKTFSIPHGGGGPAGAPICVKSHLIPH
LPKHDVVDMITGIGGSKSIDSVSSAPYGNALVLPISYAYIKMMGNEGLPFSSVIAMLN
SNYMMTRLKDHYKILFVNEMSTLKHCAHEFIVDLREYKAKGVEAIDVAKRLQDYGFHA
PTLAFPVPGTLMIEPTESENLEELDRFCDAMISIKEEINALVAGQPKGQILKNAPHSL
EDLITSSNWDTRGYTREEAAYPLPFLRYNKFWPTVARLDDTYGDMNLICTCPSVEEIA
NETE"
gene complement(606202..610545)
/gene="Sgs1"
/locus_tag="P301_M12491"
mRNA complement(606202..610545)
/gene="Sgs1"
/locus_tag="P301_M12491"
/product="Sgs1p"
CDS complement(606202..610545)
/gene="Sgs1"
/locus_tag="P301_M12491"
/note="Similar to YMR190C 'Nucleolar DNA helicase of the
RecQ family involved in genome integrity maintenance;
regulates chromosome synapsis and meiotic joint
molecule/crossover formation; similar to human BLM and WRN
proteins implicated in Bloom and Werner syndromes'"
/codon_start=1
/product="Sgs1p"
/protein_id="EWG89459.1"
/translation="MVTKPSHNLRREHKWLKETATLQEDKDFVFQAIQKHIANKRPKT
NSPPTTPSKDECGPGTTNFITSIPASGPTNTATKQHEVMQTLSNDTEWLSYPATSNQY
ADVPMVDIPASTSVVSNPRTPNGSKTHNFNTFRPHMASSLVENDSSRNLGSRNNNKSV
IDNSSIGKQLENDIKLEVIRLQGSLIMALKEQSKLLLQKCSIIESTSLSEDAKRLQLS
RDIRPQLSNMSIRIDSLEKEIIKAKKDGMSKDQSKGRSQVSSQDDNIISSILPSPLEY
NTSSRNSNLTSTTATTVTKALAITGAKQNITNNTGKNSNNDSNNDDLIQVLDDEDDID
CDPPVILKEGAPHSPAFPHLHMTSEEQDELTRRRNMRSREPVNYRIPDRDDPFDYVMG
KSLRDDYPDVEREEDELTMEAEDDAHSSYMTTKDEEKEENELLNQSDFDFVVNDDLDP
TQDTDYHDNMDVSANIQESSQEGDTRSTITLSQNKNVQVILSSPTAQSVPSNGQNQIG
VEHIDLLEDDLEKDAILDDSMSFSFGRQHMPMSHSDLELIDSEKENEDFEEDNNNNGI
EYLSDSDLERFDEERENRTQVADIQELDNDLKIITERKLTGDNEHPPPSWSPKIKREK
SSVSQKDEEDDFDDDFSLSDIVSKSNLSSKTNGPTYPWSDEVLYRLHEVFKLPGFRPN
QLEAVNATLQGKDVFVLMPTGGGKSLCYQLPAVVKSGKTHGTTIVISPLISLMQDQVE
HLLNKNIKASMFSSRGTAEQRRQTFNLFINGLLDLVYISPEMISASEQCKRAISRLYA
DGKLARIVVDEAHCVSNWGHDFRPDYKELKFFKREYPDIPMIALTATASEQVRMDIIH
NLELKEPVFLKQSFNRTNLYYEVNKKTKNTIFEICDAVKSRFKNQTGIIYCHSKKSCE
QTSAQMQRNGIKCAYYHAGMEPDERLSVQKAWQADEIQVICATVAFGMGIDKPDVRFV
YHFTVPRTLEGYYQETGRAGRDGNYSYCITYFSFRDIRTMQTMIQKDKNLDRENKEKH
LNKLQQVMAYCDNVTDCRRKLVLSYFNEDFDSKLCHKNCDNCRNSANVINEERDVTEP
AKKIVKLVESIQNERVTIIYCQDVFKGSRSSKIVQANHDTLEEHGIGKSMQKSEIERI
FFHLITIRVLQEYSIMNNSGFASSYVKVGPNAKKLLTGKMEIKMQFTISAPNSRPSTS
SSFQSNEDNIPVIAQKSTTIGGNVAANPPRFISAKEHLRSYTYGGSTMGSSHPITLKN
TSDLRSTQELNNLRMTYERLRELSLNLGNRMVPPVGNFMPDSILKKMAAILPMNDSAF
ATLGTVEDKYRRRFKYFKATIADLSKKRSSEDHEKYDTILNDEFVNRAAASSNGIAQS
TGTKSKFFGANPNEAKENEQIINQIRQSQLPKNTTSSKSGTRSISKSSKKSANGRRGF
RNYRGHYRGRK"
gene 610944..612065
/gene="Spg5"
/locus_tag="P301_M12496"
mRNA 610944..612065
/gene="Spg5"
/locus_tag="P301_M12496"
/product="Spg5p"
CDS 610944..612065
/gene="Spg5"
/locus_tag="P301_M12496"
/note="Similar to YMR191W 'Protein required for survival
at high temperature during stationary phase; not required
for growth on nonfermentable carbon sources'"
/codon_start=1
/product="Spg5p"
/protein_id="EWG89460.1"
/translation="MAVGGNNWSMWLRMSRVHLRQITKSLDRTLISLSHGNFSHQYNH
NIFVTWWKSLFEASTAFRRASGLTVSPLTRRGIARFDRFRPVANVSKFASFPRVPKGA
PRGLFTNWNMTTSKRLLGQRAYSTSSIKFTQEAVNNMTISLRCFFNSLGGLNQCSHSN
SCKAYQNASNVTSKQDHVQPVALKKLSQKDINFIRDLELFKIMKTQNEVVDETSAYYM
EKPGSYIEFTISEFNVNGTFSAPLSFLDPSLLADLDEMIRNYKYELKSIYSSVDMILQ
NYGSLPITFHRNKIRIHFPNSTVVETEKLIAGLNIATGVIYADTSPDISLEGTNLNAL
VNVDNSGSVWSFVNEPSFPSRSAFSPILSDASYDTYELV"
gene 612394..614556
/gene="Gyl1"
/locus_tag="P301_M12506"
mRNA 612394..614556
/gene="Gyl1"
/locus_tag="P301_M12506"
/product="Gyl1p"
CDS 612394..614556
/gene="Gyl1"
/locus_tag="P301_M12506"
/note="Similar to YMR192W 'Putative GTPase activating
protein (GAP) that may have a role in polarized
exocytosis; stimulates Gyp5p GAP activity on Ypt1p,
colocalizes with Gyp5p at sites of polarized growth;
interacts with Gyp5p, Rvs161p, and Rvs167p'"
/codon_start=1
/product="Gyl1p"
/protein_id="EWG89461.1"
/translation="MNSNEDIHEERIEVPRTPHQTQPEKDSDRIALRDEISVPEGDEK
AYSDEKVEMATTNASSNFGSNESAKDGESIGAFSNPHEALMQSKLREESQSKTILPSD
DLSQQLETEESKVEEALKRITSPPLPPRADCIEESASALKSSLPPVLAGNKNDQAPLD
RPQLPPRQVVNAETLHLKAPHGNATPSKSPTSAVGNSSSSTPPTLPPRRIEDPLDLAA
QKHFLASTFKRNMLFYKSEDNSIKCDLDKNILNLKEDSKKINNNEIPEEVSSFWLKVI
GDYQNILINDIETLHFQLSRGIPAAYRLVVWQLVSYAKSKSFDPIYETYLTEMAPFDV
QEFENQLKMMDEVPSENVKRISNVLKAYLLFDPECEFSTDIAYIINMILDVCEEEANA
FGLLVRLMKVYGLRLLFLPSASEIDILCYKFDRLVEEFYPEIHNHMVEKGVRSSMFLP
GFFTTLFQKKLPTEIQPRIGDMVFLEGIDSIMRILATLLSNSRDHLLKMGFDDMLELL
KSGLLDVYIKQNDGTRGDTLLSNECMDKLLQDSMMKVAITPKTMKKYSSEYEEIHRLD
NEKEVQYKSITEKNLHLQKHVRKLENDYTSLNREHVTIANELVKNRLNIESVLNENNG
YKLQILDLKKKLDSEKKKQVLGVYVPNDLKKDLEETMKKNTQVMDENLKLQDRISELE
RLIEEIKTANKNGTLFEYSNSKNNPLGAGWSGFKKVFK"
gene 615312..616088
/gene="Mrpl24"
/locus_tag="P301_M12511"
mRNA 615312..616088
/gene="Mrpl24"
/locus_tag="P301_M12511"
/product="Mrpl24p"
CDS 615312..616088
/gene="Mrpl24"
/locus_tag="P301_M12511"
/note="Similar to YMR193W Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl24p"
/protein_id="EWG89462.1"
/translation="MQKIFRPFQLTRGFTSSVKNFRQWRLIETRKIAKQPNYQVGDAK
PLHMPKERKKFPDYKYGESNIFKQSNKGLYGGSFVQFGNNISESKAKTRKKWLPNVVK
KGLWSETLNRKISIKMTAKVLKTISKEGGIDNYLTKEKSARIKELGPTGWKLRYRVLK
RKDEIENPPHKDAPIIEMAGGKKAKIYYDEIVNGSPRKISVGRRRLMSFLYPLEKLEY
RSVGKDLNHKKFVELFADVPVKDILARLEDHKFDLSTITV"
gene complement(616349..616735)
/locus_tag="P301_M12516"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 616421..617185
/gene="Rpl36a"
/locus_tag="P301_M12521"
mRNA join(616421..616436,616899..617185)
/gene="Rpl36a"
/locus_tag="P301_M12521"
/product="Rpl36ap"
CDS join(616421..616436,616899..617185)
/gene="Rpl36a"
/locus_tag="P301_M12521"
/note="Similar to YMR194W 'N-terminally acetylated protein
component of the large (60S) ribosomal subunit, nearly
identical to Rpl36Bp and has similarity to rat L36
ribosomal protein; binds to 5.8 S rRNA'"
/codon_start=1
/product="Rpl36ap"
/protein_id="EWG89463.1"
/translation="MAVKTGIAIGLNKGKKVTSMTPAPKISYKKGAASNRTKFVRSLV
REIAGLSPYERRLIDLIRNSGEKRARKVAKKRLGSFTRAKAKVEEMNNIIAASRRH"
gene 617548..617805
/locus_tag="P301_M12531"
ncRNA 617548..617805
/ncRNA_class="snoRNA"
/locus_tag="P301_M12531"
/product="SNR11"
gene complement(617866..618159)
/locus_tag="P301_M12541"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(618184..618405)
/locus_tag="P301_M12546"
mRNA complement(618184..618405)
/locus_tag="P301_M12546"
/product="hypothetical protein"
CDS complement(618184..618405)
/locus_tag="P301_M12546"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89464.1"
/translation="MPVANIWRLLHVNDVPTINFARSGRALLTSSVSETFTDITFHPW
GVYESSEEGVERQNITVLFFQDIERFFSC"
gene 619316..619696
/gene="Icy1"
/locus_tag="P301_M12556"
mRNA 619316..619696
/gene="Icy1"
/locus_tag="P301_M12556"
/product="Icy1p"
CDS 619316..619696
/gene="Icy1"
/locus_tag="P301_M12556"
/note="Similar to YMR195W 'Protein of unknown function,
required for viability in rich media of cells lacking
mitochondrial DNA; mutants have an invasive growth defect
with elongated morphology; induced by amino acid
starvation'"
/codon_start=1
/product="Icy1p"
/protein_id="EWG89465.1"
/translation="MSSNYATPLDDEVFPLSFANYQFTEHVSLGEHYSLNTSEDAKYN
LNGPFVVPRDTGKFDLNTSSASDETVFSLDNPQENNYKHQAMNNVQDCRMAVAAKTTQ
SCDKLTDLYANAAQQNYRLWLSSF"
gene 620355..623623
/locus_tag="P301_M12566"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 620899..620988
/estimated_length=90
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(623826..624479)
/gene="Vti1"
/locus_tag="P301_M12576"
mRNA complement(623826..624479)
/gene="Vti1"
/locus_tag="P301_M12576"
/product="Vti1p"
CDS complement(623826..624479)
/gene="Vti1"
/locus_tag="P301_M12576"
/note="Similar to YMR197C 'Protein involved in cis-Golgi
membrane traffic; v-SNARE that interacts with two
t-SNARES, Sed5p and Pep12p; required for multiple vacuolar
sorting pathways'"
/codon_start=1
/product="Vti1p"
/protein_id="EWG89466.1"
/translation="MSSLLISYESDFKTTLEQAKASLAEAPSQPLSQRNTTLKHVEQQ
QDELFDLLDQMDVEVNNSIGDASERATYKAKLREWKKTIQSDIKRPLQSLVDSGDRDR
LFGDLNASNIDDDQRQQLLSNHAILQKSGDRLKDASRIANETEGIGSQIMMDLRSQRE
TLENARQTLFQADSYVDKSIKTLKTMTRRLVANKFISYAIIAVLILLILLVLFSKFK"
gene 625026..626810
/gene="Cik1"
/locus_tag="P301_M12581"
mRNA 625026..626810
/gene="Cik1"
/locus_tag="P301_M12581"
/product="Cik1p"
CDS 625026..626810
/gene="Cik1"
/locus_tag="P301_M12581"
/note="Similar to YMR198W 'Kinesin-associated protein
required for both karyogamy and mitotic spindle
organization, interacts stably and specifically with Kar3p
and may function to target this kinesin to a specific
cellular role; has similarity to Vik1p'"
/codon_start=1
/product="Cik1p"
/protein_id="EWG89467.1"
/translation="MNNSKIPKLSFHSDPNNVTRDFPKTKRQKVQKREMDMILTPNNN
KLNILHSSGSGIRRCYTDDTSATYTKKLTFGGDPKVIERVKNNERKVRKDIDSLLNAI
SEIEKESVRIHARELPAITLELDAKVKACRELQNEIDGLSTEMDLKDNQCDLQRKNVE
LSSKNIVSMHAVKVQEFENDLEEELSNAKREWTYKLMEVENLKPDERLTDEMRQLKTE
FEEVNRKLFILQNENENECKNYKKELDKKFEIFKKVKNDARIELDGEQERLSKVLKDL
QDTHGELKENIKTCRDEFNDFEKRIGEAEVNFHSMELAVVPLKKKLASTSQALTQVQE
EKKQVEGEANNWKKKYVNELEKVQQELYTRQNLATSIEEIKGYTRCFAYANERQMPDE
FHINYVDRCICENSGEKRVQVFDRVVLEEIHKDHKRLYNECIPFLEKYISKLINCSII
VVSQQPTAPMKKTLLKQLIEQYGENYKMTLNILHLDGSIKHSDVGLDNPTEIRDLSQD
EECMNILTLDTKLRKDEESHSMNIYIGSMSTVQLNRELDDAPSVLSHILTKTKQCFVF
KINAGENIEKALALAGKLKRTITLPQLD"
gene 627923..629563
/gene="Cln1"
/locus_tag="P301_M12586"
mRNA 627923..629563
/gene="Cln1"
/locus_tag="P301_M12586"
/product="Cln1p"
CDS 627923..629563
/gene="Cln1"
/locus_tag="P301_M12586"
/note="Similar to YMR199W 'G1 cyclin involved in
regulation of the cell cycle; activates Cdc28p kinase to
promote the G1 to S phase transition; late G1 specific
expression depends on transcription factor complexes, MBF
(Swi6p-Mbp1p) and SBF (Swi6p-Swi4p)'"
/codon_start=1
/product="Cln1p"
/protein_id="EWG89468.1"
/translation="MNHSEVKTGLIVTAKQTYYPIELSNAELLTHYETIQEYHEEISQ
NVLVQSSKTKPDIKLIDQQPEMNPHQTREAIVTFLYQLSVMTRVSNGIFFHAVRFYDR
YCSKRVVLKDQAKLVVGTCLWLAAKTWGGCNHIINNVSIPTGGRFYGPNPRARIPRLS
ELVHYCGGSDLFDESMFIQMERHILDTLNWDVYEPMINDYILNVDENCLIQYELYKNQ
LQNNNSNGKEWSCKRKSQSSDDSDATVEEHISSSPQSTGLDGDTTTMDEDEELNSKIK
LINLKRFLIDLSCWQYNLLKFELYEICNGMFSIINKFTNQDQGPFLSMPIGNDINSNT
QTQVFSIIINGIVNSPPSLVEVYKEQYGIVPFILQVKDYNLELQKKLQLASTIDLTRK
IAVNSRYFDQNASSSSVSSPSTYSSGTNYTPMRNFSAQSDNSVFSTTNIDHSSPITPH
MYTFNQFKNESACDSAISVSSLPNQTQNGNMPLSSNYQNMMLDERNKENRIPNSSSAE
IPQRAKFMTTGIFQNTGELTNRASSISLSLRNHNSSQL"
gene 630031..630801
/gene="Rot1"
/locus_tag="P301_M12596"
mRNA 630031..630801
/gene="Rot1"
/locus_tag="P301_M12596"
/product="Rot1p"
CDS 630031..630801
/gene="Rot1"
/locus_tag="P301_M12596"
/note="Similar to YMR200W 'Molecular chaperone involved in
protein folding in the ER; mutation causes defects in cell
wall synthesis and in lysis of autophagic bodies,
suppresses tor2 mutations, and is synthetically lethal
with kar2-1 and with rot2 mutations'"
/codon_start=1
/product="Rot1p"
/protein_id="EWG89469.1"
/translation="MWSKKFTLKKLILGGYLFAQKVYCEDESNSIYGTWSSKSNQVFT
GPGFYDPVDELLIEPSLPGLSYSFTEDGWYEEATYQVSGNPRNPTCPMASLIYQHGTY
NISENGTLVLNPIEVDGRQLFSDPCNDDGVSTYSRYNQTETFKEYAVGIDPYHGIYTL
QLYQYDGTPMQPLYLAYRPPMMLPTETLNPTSSATSTDDPSSNKKRSLRSLVRRSLEN
RHKTNAIKRQNTSFLTSNAIWYISAGMLGVGSLLFLAF"
gene complement(631124..632323)
/gene="Rad14"
/locus_tag="P301_M12601"
mRNA complement(join(631124..632212,632297..632323))
/gene="Rad14"
/locus_tag="P301_M12601"
/product="Rad14p"
CDS complement(join(631124..632212,632297..632323))
/gene="Rad14"
/locus_tag="P301_M12601"
/note="Similar to YMR201C 'Protein that recognizes and
binds damaged DNA during nucleotide excision repair;
subunit of Nucleotide Excision Repair Factor 1 (NEF1);
contains zinc finger motif; homolog of human XPA protein'"
/codon_start=1
/product="Rad14p"
/protein_id="EWG89470.1"
/translation="MTPEQKAKLEANRKLAIERLRKRGILSSDQLNRIESRNEPLKTR
PLAVTSGSNRDDNAAAAVHVPNHNGQPSALANTNTNTTSLYGSGVVDGSKRDASVLDK
RPTDRIRPSIRKQDYIEYDFATMQNLNGGYINPKDKLPNSDFTDDQEFESEFGSKKQK
TLQDWKKEQLERKMLYENAPPPEHISKAPKCIECHINIEMDPVLHDVFKLQVCKQCSK
EHPEKYALLTKTECKEDYFLTDPELNDEDLFHRLEKPNPHSGTFARMQLFVRCEVEAF
AFKKWGGEEGLDEEWQRREEGKAHRREKKYEKKIKEMRLKTRAQEYTNRLREKKHGKA
HIHHFSDPVDGGIDEDGYQIQRRRCTDCGLETEEIDI"
gene complement(632557..632725)
/locus_tag="P301_M12606"
ncRNA complement(632557..632725)
/ncRNA_class="snoRNA"
/locus_tag="P301_M12606"
/product="SNR11"
gene 632806..633474
/gene="Erg2"
/locus_tag="P301_M12611"
mRNA 632806..633474
/gene="Erg2"
/locus_tag="P301_M12611"
/product="Erg2p"
CDS 632806..633474
/gene="Erg2"
/locus_tag="P301_M12611"
/note="Similar to YMR202W C-8 sterol isomerase, catalyzes
the isomerization of the delta-8 double bond to the
delta-7 position at an intermediate step in ergosterol
biosynthesis"
/codon_start=1
/product="Erg2p"
/protein_id="EWG89471.1"
/translation="MKFFPLLLLIGVVGYIMNVLFTTWLPTNYMFDPKTLNEICNSVI
SKHNAAEGLSTEDLLQDVRDALASHYGDEYINRYVKEEWVFNNAGGAMGQMIILHASV
SEYLILFGTAVGTEGHTGVHFADDYFTILHGTQIAALPYATEAEVYTPGMTHHLKKGY
AKQYSMPGGSFALELAQGWIPCMLPFGFLDTFSSTLDLYTLYRTVYLTARDMGKNLLQ
NKKF"
gene 633763..634926
/gene="Tom40"
/locus_tag="P301_M12616"
mRNA 633763..634926
/gene="Tom40"
/locus_tag="P301_M12616"
/product="Tom40p"
CDS 633763..634926
/gene="Tom40"
/locus_tag="P301_M12616"
/note="Similar to YMR203W 'Component of the TOM
(translocase of outer membrane) complex responsible for
recognition and initial import steps for all
mitochondrially directed proteins; constitutes the core
element of the protein conducting pore'"
/codon_start=1
/product="Tom40p"
/protein_id="EWG89472.1"
/translation="MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDT
YKQLHSHRQSLELVNPGTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSH
TFSIGSQALPKYAFSALFANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDG
QPTMCQLEQDYQASDFSVNVKTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYS
RTDGSAPGDAGVSYLTRYVSKKQDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQ
AGMVPITDPLMGTPIGIQPTVEGSTTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLP
TLSVLFCGEIDHFKNDTKIGCGLQFETAGNQELLMLQQGLDADGNPLQALPQL"
assembly_gap 635199..635236
/estimated_length=38
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(635334..636596)
/gene="Inp1"
/locus_tag="P301_M12621"
mRNA complement(635334..636596)
/gene="Inp1"
/locus_tag="P301_M12621"
/product="Inp1p"
CDS complement(635334..636596)
/gene="Inp1"
/locus_tag="P301_M12621"
/note="Similar to YMR204C 'Peripheral membrane protein of
peroxisomes involved in peroxisomal inheritance;
recruitment to peroxisomes is mediated by interaction with
Pex3p at the peroxisomal membrane'"
/codon_start=1
/product="Inp1p"
/protein_id="EWG89473.1"
/translation="MVLSRGETKKNSVRLTAKQEKKPQSTFQTLKQSLKLSNNKKLKQ
DSTQHSNDTNKSVKAKKNGTSSKKTGTQRKRISTQRFSLFTYGNVQVMNSFVPIHNDI
PNSSCIRRNSQVSANNVTESSGVFFNDTQSQDSQNTIKLKPTSLMAKGPIEIYQICTG
FDKLKENIAPFQKSSKASSHDGHVVNYLSIGRHGDIVHPVLPKLQITRLNGAGFKYFI
SFYNPERYWEIEFLPLISQSQSELENSVKAFENVISKICQFSHINEGATIGNNESLSD
KFKLPPTSDIEPPNTEIINNDDDNDDDDDNYDDDDLNYLLDEEYEQGCTDNSFSVISN
TCSNLNASFLYPSDPTDAVSISINEAFKNAIRRTAPVLNIPIAAPSIHSKQQNKRYSS
YPFIDSPPYLQDRHRRFQRRSISGLGDL"
gene complement(637158..640037)
/gene="Pfk2"
/locus_tag="P301_M12626"
mRNA complement(637158..640037)
/gene="Pfk2"
/locus_tag="P301_M12626"
/product="Pfk2p"
CDS complement(637158..640037)
/gene="Pfk2"
/locus_tag="P301_M12626"
/note="Similar to YMR205C Beta subunit of heterooctameric
phosphofructokinase involved in glycolysis, indispensable
for anaerobic growth, activated by
fructose-2,6-bisphosphate and AMP, mutation inhibits
glucose induction of cell cycle-related genes"
/codon_start=1
/product="Pfk2p"
/protein_id="EWG89474.1"
/translation="MTVTTPFVNGTSYCTVTAYSVQSYKAAIDFYTKFLSLENRSSPD
ENSTLLSNDSISLKILLRPDEKINKNVEAHLKELNSITKTQDWRSHATQSLVFNTSDI
LAVKDTLNAMNAPLQGYPTELFPMQLYTLDPLGNVVGVTSTKNAVSTKPTPPPAPEAS
AESGLSSKVHSYTDLAYRMKTTDTYPSLPKPSNRPQKAIAVMTSGGDAPGMNSNVRAI
VRSAIFKGCRAFVVMEGYEGLVRGGPEYIKEFHWEDVRGWSAEGGTNIGTARCMEFKK
REGRLLGAQHLIEAGVDALIVCGGDGSLTGADLFRSEWPSLIEELLKTNRISNEQYER
MKHLNICGTVGSIDNDMSTTDATIGAYSALDRICKAIDYVEATANSHSRAFVVEVMGR
NCGWLALLAGIATSADYIFIPEKPATSSEWQDQMCDIVSKHRSRGKRTTIVVVAEGAI
AADLTPISPSDVHKVLVDRLGLDTRITTLGHVQRGGTAVAYDRILATLQGLEAVNAVL
ESTPDTPSPLIAVNENKIVRKPLMESVKLTKAVAEAIQAKDFKRAMSLRDTEFIEHLN
NFMAINSADHNEPKLPKDKRLKIAIVNVGAPAGGINSAVYSMATYCMSQGHRPYAIYN
GWSGLARHESVRSLNWKDMLGWQSRGGSEIGTNRVTPEEADLGMIAYYFQKYEFDGLI
IVGGFEAFESLHQLERARESYPAFRIPMVLIPATLSNNVPGTEYSLGSDTALNALMEY
CDVVKQSASSTRGRAFVVDCQGGNSGYLATYASLAVGAQVSYVPEEGISLEQLSEDIE
YLAQSFEKAEGRGRFGKLILKSTNASKALSATKLAEVITAEADGRFDAKPAYPGHVQQ
GGLPSPIDRTRATRMAIKAVGFIEDNQAAIAEARAAEENFNADDKTISDTAAVVGVKG
SHVVYNSIRQLYDYETEVSMRMPKVIHWQATRLIADHLVGRKRVD"
gene 641163..642104
/locus_tag="P301_M12636"
mRNA 641163..642104
/locus_tag="P301_M12636"
/product="hypothetical protein"
CDS 641163..642104
/locus_tag="P301_M12636"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89475.1"
/translation="MLSSSSNRPISAHLTIHYKAIQEEEGEDMRSGAGSGGHHDDYFL
ESNRSPTPNKKHEFIKTVLNINDNDSEFSESCSPREKLHNERACNTDLFGDFMSKRQQ
RLSNSMNIYDLYQCVHNLSPSNNNHQFIARRFSDSHIPSLHHRQQQQKVTTKNFVQPT
KDIQRIASYAADSDQRVKYLPNYHQSAPSTALSAAESKAAVPRKLPDRDSTQNYVLKL
QLSSPNSQPMSPRTRPGYRPSCSSSNCSSSSSSSACSSVSISDPNNITAYETNNVNPQ
FPNNQPLDISSPCARHHHRRNSIAVKFDKALYKKTTG"
gene complement(642460..648802)
/gene="Hfa1"
/locus_tag="P301_M12641"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 647711..647788
/estimated_length=78
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 649705..651036
/gene="Erg12"
/locus_tag="P301_M12651"
mRNA 649705..651036
/gene="Erg12"
/locus_tag="P301_M12651"
/product="Erg12p"
CDS 649705..651036
/gene="Erg12"
/locus_tag="P301_M12651"
/note="Similar to YMR208W Mevalonate kinase, acts in the
biosynthesis of isoprenoids and sterols, including
ergosterol, from mevalonate"
/codon_start=1
/product="Erg12p"
/protein_id="EWG89476.1"
/translation="MSLPFLTSAPGKVIIFGEHSAVYNKPAVAASVSALRTYLLISES
SAPDTIELDFPDISFNHKWSINDFNAITEDQVNSQKLAKAQQATDGLSQELVSLLDPL
LAQLSESFHYHAAFCFLYMFVCLCPHAKNIKFSLKSTLPIGAGLGSSASISVSLALAM
AYLGGLIGSNDLEKLSENDKHIVNQWAFIGEKCIHGTPSGIDNAVATYGNALLFEKDS
HNGTINTNNFKFLDDFPAIPMILTYTRIPRSTKDLVARVRVLVTEKFPEVMKPILDAM
GECALQGLEIMTKLSKCKGTDDEAVETNNELYEQLLELIRINHGLLVSIGVSHPGLEL
IKNLSDDLRIGSTKLTGAGGGGCSLTLLRRDITQEQIDSFKKKLQDDFSYETFETDLG
GTGCCLLSAKNLNKDPKIKSLVFQLFENKTTTKQQIDDLLLPGNTNLPWTS"
gene complement(651148..652521)
/locus_tag="P301_M12656"
mRNA complement(651148..652521)
/locus_tag="P301_M12656"
/product="hypothetical protein"
CDS complement(651148..652521)
/locus_tag="P301_M12656"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89477.1"
/translation="MVSSLASNIILALVVVLMTLLRQNKPLQKWFVSYVEKLLSRKSN
LAQKVSVLSPSLKLVDVEKSISADQSNLSKINYQIPTLPDLKGNLIRLHEYRARGEGY
NGLLFRRARQLNGISEDQLQKLGYFTKLMKNNEGIRENARVIDKIIEFTLGKLIHSNE
HDEEFTEEIEKICAEHGYKIKDGHLIQLNPDFVFPIVSSRGSQSVVHEALAHLCRDFS
SYYSVERDPLQNFIISRINHHVISAGDMKEKILIVTPGAGVGGLSHTLATTFPKIQVD
SIELSALMYICNLFALEYKHDVKIRPFVQQYSCQTVFDNQLRSLSADLSKVGHRSNLD
PLWGDFTRYSPISKDYDKIIICSAYFIDTAENMFEYLSSIEALKKYCKELHWVNVGPL
KYGTKPLVQFTGDELSRLRKIRGWKDLVEEYEVDSSKGLNGYLTDYESMYQGYYGLLK
FHSVFES"
gene 652752..654101
/locus_tag="P301_M12661"
mRNA 652752..654101
/locus_tag="P301_M12661"
/product="hypothetical protein"
CDS 652752..654101
/locus_tag="P301_M12661"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89478.1"
/translation="MRLKELLPNFLIVHQEVPEDPIAFKSTDKRENENKEITIPELID
TKVPELADGATDTLYGLLVNGHLQTAYGSFRHFDNIYKVQYKRMIIKYPHGGEGTVDF
AVNGRSTKRRKVEKEYVPTSQPVFNGNLKRRYSYYSPDDPKLNSDDAKPMLIILHGLT
GGSRESYVRAIVHEITTKYDFEACVFNARGCCYSAITTPLLYNGGWTNDIRYCVNDLR
KRFPNRKFYMMGFSLGASIMTNYLGEESDRTKIECAISVSNPFDLYNSAYFINSTPMG
SRFYSPALGHNLLRMVRNHLSTLEENPDFKDVIEKHLKKIRTVRQFDNLLTGPMFGYK
NAEEYYKNASSYKRIPGIRTPFIALHAQDDPIVGGDLPIDQIKSNPYTLLLETSTGGH
VGWFKDRSGRRWYAEPLCRFLKIFHDEITVKGLKPDLENVQLPDPNCEPIATTFRAN"
gene 654318..655745
/gene="Dml1"
/locus_tag="P301_M12666"
mRNA 654318..655745
/gene="Dml1"
/locus_tag="P301_M12666"
/product="Dml1p"
CDS 654318..655745
/gene="Dml1"
/locus_tag="P301_M12666"
/note="Similar to YMR211W Essential protein involved in
mtDNA inheritance, may also function in the partitioning
of the mitochondrial organelle or in the segregation of
chromosomes, exhibits regions similar to members of a
GTPase family"
/codon_start=1
/product="Dml1p"
/protein_id="EWG89479.1"
/translation="MHEVVTISVSQRANHLTTQFFNIQEGYLQLSKEQQVNDSKIFLN
SVVDKVSKTISYAPRALLWDARTGNGSLGTYQYSESQDYHFGNEDKFKEQTVIKTHPR
IPKSEYQSSLDAGAPLPCLNRENTMYWSDYSKLIYGPSSFNILRNWYHDTENPNQPDF
QNLGERKFDRYSIGYDEFTENYLQEFFDGNLHRELEKCDTLQGFNLVSDMESGWGGFS
SALLVELRNELPKKAVFSWGHNEDDPFTDDFPMKRLSKKWLPIISNKLRSTINMMQES
DLYFPLYAAPGLTNWETAGKSCRIFDSINATISQSNLEQRKTMDYLTTAITLGYSSRN
MVTGMVIGDTDYSFCSRVLPFKNSHKPNSTHIFSKSFIDRGNQTHKHHSEPDSRSKMI
EMYTHRYFPSDTIPTEFSNDREFVLELESSEKNRDIFKHWNEFVVRYFKNDSDREELK
NELSDYASAYESGWYEDEDSGDDDM"
gene complement(655930..658277)
/gene="Efr3"
/locus_tag="P301_M12671"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 658615..660387
/gene="Cef1"
/locus_tag="P301_M12676"
mRNA 658615..660387
/gene="Cef1"
/locus_tag="P301_M12676"
/product="Cef1p"
CDS 658615..660387
/gene="Cef1"
/locus_tag="P301_M12676"
/note="Similar to YMR213W 'Essential splicing factor;
associated with Prp19p and the spliceosome, contains an
N-terminal c-Myb DNA binding motif necessary for cell
viability but not for Prp19p association, evolutionarily
conserved and homologous to S. pombe Cdc5p'"
/codon_start=1
/product="Cef1p"
/protein_id="EWG89480.1"
/translation="MPPVPIYVKGGVWTNVEDQILKAAVQKYGTHQWSKVASLLQKKT
ARQSELRWNEYLNPKLNFTEFSKEEDAQLLDLARELPNQWRTIADMMARPAQVCVERY
NRLLESEDSGGAALSTGVTDLKVGDINPNAETQMARPDNGDLEDEEKEMLAEARARLL
NTQGKKATRKIRERMLEESKRIAELQKRRELKQAGINVAIKKPKKKYGTDIDYNEDIV
YEQAPMPGIYDTSTEDRQIKKKFEQFERKVNRKGLDGNKDKPSKKNKDKKRKHDENEH
VEKAALGESTTLTDEYKKPKLILSAPGTKQGKVTYKKKLESKRQKLIEAQATGTVLTP
KELLPHDSGQEDNERSNIKSGKQLKSRIRKFLVQMFASLPSPKNDFEIVLSEDEKEED
AEIAEYEKEFENERAMNEEDNFIEPPSQNDAPRVSLVAVPLAYSTLPIPEFKNNPQSA
IDNKYNLLVANAINKEPHMVPEDTVDFLKEVESRMQHITQGRTSMKIQFKTAMPPTEV
LLESIQSKVESIEQLQRKLQHVQPLEQQNNEMCSTLCHHSLPALIEGQRKYYADYYAY
RQEIRSLEGRRKRLQAMLNSSSSI"
gene 660585..661718
/gene="Scj1"
/locus_tag="P301_M12681"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 662030..663605
/gene="Gas3"
/locus_tag="P301_M12686"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(664044..666272)
/gene="Sky1"
/locus_tag="P301_M12696"
mRNA complement(664044..666272)
/gene="Sky1"
/locus_tag="P301_M12696"
/product="Sky1p"
CDS complement(664044..666272)
/gene="Sky1"
/locus_tag="P301_M12696"
/note="Similar to YMR216C 'SR protein kinase (SRPK)
involved in regulating proteins involved in mRNA
metabolism and cation homeostasis; similar to human
SRPK1'"
/codon_start=1
/product="Sky1p"
/protein_id="EWG89481.1"
/translation="MGSSINYPGFVTKSAHLADTSTDASISCEEATSSQEAKKNFFQR
DYNMMKKAPAPTKSKLSLALQTSKSSSSANGTVQEDTSSKTEDFSTKSIKKKPDSGVG
SHVSIQSDSGPQSDSDLDSDSSISSCDERNEESLKDYRPGGYHPAFKGEPYKDARYIL
VRKLGWGHFSTVWLAKDMVNNTHVAMKIVRSDKVYTEAAEDEIKLLQRVNDADNTKED
SMGANHILKLLDHFNHKGPNGVHVVMVFEVLGENLLALIKKYEHRGIPLIYVKQISKQ
LLLGLDYMHRRCGIIHTDIKPENVLMEIGDVEGIVQMVEALDKQKREAKRLQRHVSRS
SDITANDSSDEKWAECQTSMPCGSNSNSKSRSIEKDLSKRCFRRPRRHTIITGSQPLP
SPISSSNFFEMRAHFCGSSHNSFSSVSGNRNIPSSINNNSINNGIGIKNSNNSFLNSV
PHSVTRMFINEDSNDNNNNDNSKTKNNNNNNSNNNNNEDIMNTPLHEEQLADSLSTFD
ISNISQSSDTNGPYISNTMDSNSNVSTDINSPESLIQIKIADLGNACWYDEHYTNSIQ
TREYRSPEVLLGAPWGCGADIWSTACLIFELITGDFLFEPDEGHSYTKDDDHIAQIIE
LLGELPSYLLRNGKYTRTFFNSRGLLRNISKLKFWPLKDVLTEKYKFSKDEAKEISDF
LSPMLQLDPRKRADAGGLVNHPWLKDTLGMEEIRVPDRELYGSGSDIPGWFEEVRNHK
RH"
gene 667021..668658
/gene="Gua1"
/locus_tag="P301_M12701"
mRNA 667021..668658
/gene="Gua1"
/locus_tag="P301_M12701"
/product="Gua1p"
CDS 667021..668658
/gene="Gua1"
/locus_tag="P301_M12701"
/note="Similar to YMR217W 'GMP synthase; highly conserved
enzyme that catalyzes the second step in the biosynthesis
of GMP from inosine 5'-phosphate (IMP); transcription is
not subject to regulation by guanine but is negatively
regulated by nutrient starvation; reduction-of-function
mutation gua1-G388D causes changes in cellular guanine
nucleotide pools, defects in general protein synthesis,
and impaired translation of GCN4 mRNA'"
/codon_start=1
/product="Gua1p"
/protein_id="EWG89482.1"
/translation="MAAGEQVSNMFDTILVLDFGSQYSHLITRRLREFNIYAEMLPCT
QKISELGWTPKGVILSGGPYSVYAEDAPHVDHAIFDLNVPILGICYGMQELAWINGKQ
VGRGDKREYGPATLKVIDDSNSLFKGMNDSTVWMSHGDKLHGLPTGYKTIATSDNSPY
CGIVHETKPIYGIQFHPEVTHSTQGKTLLKNFAVDLCHAKQNWTMENFIDTEINRIRK
LVGPTAEVIGAVSGGVDSTVASKLMTEAIGDRFHAILVDNGVLRLNEAANVKKTLVEG
LGINLMVVDASEEFLSKLKGVTDPEKKRKIIGNTFIHVFEREAEKIKPKDGKEIQFLL
QGTLYPDVIESISFKGPSQTIKTHHNVGGLLENMKLKLIEPLRELFKDEVRHLGELLG
IPHDLVWRHPFPGPGIAIRVLGEVTKEQVEIARKADNIYIEEIKKAGLYNQISQAFAC
LLPVKSVGVMGDQRTYDQVIALRAIETTDFMTADWFPFEHSFLKKVASRIVNEVDGVA
RVTYDITSKPPATVEWEVIHFYFNIFYIYIYISLVQI"
gene complement(668811..672119)
/gene="Trs130"
/locus_tag="P301_M12706"
mRNA complement(668811..672119)
/gene="Trs130"
/locus_tag="P301_M12706"
/product="Trs130p"
CDS complement(668811..672119)
/gene="Trs130"
/locus_tag="P301_M12706"
/note="Similar to YMR218C 'One of 10 subunits of the
transport protein particle (TRAPP) complex of the
cis-Golgi which mediates vesicle docking and fusion;
involved in ER to Golgi membrane traffic; mutation
activates transcription of OCH1'"
/codon_start=1
/product="Trs130p"
/protein_id="EWG89483.1"
/translation="MDKEIYCGSVPVSYFDPFDLFESLRPEFQQILPLDNIHWKAFDG
TVRTVNRLPIELIPEGRGEADKSNDEQPFIRFLIVNCISIDQYRAKVRPLVRQWLPNL
ESVSSSTGEKMIYKPIILLYANSEVVDSNLFKSVSLMEKFGKDFPHVQTLEVRSVYRS
PKDRQEFWNQFSQKIKASVLSIFQKRLTHLQHSLANLQKGNNFEEQLLTREKLYELYV
VFNILEDASLELQKIKKEILRRNMNMPDGKLQVPFESSSKSDESLGSIIIEGTLDKFQ
LHKYFFIRRLRLLKLEDQTLTAFVGAFQLIKNFIESISIEYRKSVRLLEFKHYFITSM
LSYFEFENVSNPLLCEIKAELLMLKRDNWVQGVMATSGYRLMDKNYPNSDVKYKFDLL
KETFVDETVFQENFLTLTKEILSLFNKCEGKRQRIVDILSIEIGLLYYQGKKYEEAVS
LFLSSYEYYTQTNWNSIGLKILQVFIDSLSHCPKLDVLQIDGESVSASAVLTNAFLNI
LKLCKDNDSKEIWWKKFMDLQMKNNIHLMYPLDGLFEVTLNSKVHLARANVSAIEVNL
KSYGFPEDISTKTMRLSLKNMGGDVIVFEASDFLLKKGENKLILECRDIMYGEFSLLS
FEIIVEGITFVKEFPENQDEFIVVPEIYCKESTKVLVKQAHNLNLGEYALELKSVQSD
ALESLQVEVEVQKNIGNMKNLPVSFSMDEIQARKRYNTPFENVRLEYYLLDQITAFDL
IIKTSFTKKNDQGTFGETKKVRIQCYLQLSVSVEDIFKKDIFFFKFLLNSSVREEPVI
LYSSELSAPDTRNDYNIRGDYIATTPALITFDGNESFINCYEITANNNFDSKDIFNLK
VRYNTLKEQLDCFITDAVLIEGDVEWFILFEKWKTFWELEILKKLKYDYDAFKENRII
RLLKTSIDLNKTKSKIRNLCIEKAVLDKILICLNKVSRGIAVCNTDMDEYVRNLVPKQ
LTVPVQLPGFEQFFHVQFEQMETSHDALHDTIATIGNSLSYTVIVENLSGQWGQDVID
DGGYIFEILSSNEWLIHGQKRCAIKEKRKEFEVHLIPLKKGYLNFPRVEITNINGKSC
RVDHSNAFESILIF"
gene 672365..677364
/gene="Esc1"
/locus_tag="P301_M12711"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 677546..678900
/gene="Erg8"
/locus_tag="P301_M12716"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(679160..680674)
/gene="Fmp42"
/locus_tag="P301_M12721"
mRNA complement(679160..680674)
/gene="Fmp42"
/locus_tag="P301_M12721"
/product="Fmp42p"
CDS complement(679160..680674)
/gene="Fmp42"
/locus_tag="P301_M12721"
/note="Similar to YMR221C 'Putative protein of unknown
function; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies;
physical interaction with Atg27p suggests a possible role
in autophagy'"
/codon_start=1
/product="Fmp42p"
/protein_id="EWG89484.1"
/translation="MTSTRTLRYAQVACACIWCLFSAGIIFGFAALKPILISEGVYHE
LCDPKDGDRLLCTAQDLKLNFIFALSATVTNIMALPVGKILDMYGPRVCGIIGSCLLF
LASGNFISAKHLVSLWDPYLVGYTLLAVAGPFVFISCFQLANSFPQRSGTVLALLTGS
FDSSSALFLLYRLLYQNWFPTLNVSRFFTLYLIVPVFILVCQLTIMPHSSYKTVNHIA
KIAVEGLDENGRLIEGDTGSGIIPDEQERQSLIAIEREEDSIPSRPQRRKSVLETYVE
DKLQKKSGGIFGVLHGKSAYEQIKSPWFYLMLLFALVAMLRINYFIATVRTQEEYLLN
DPDLALKLNSIFDMLLPLGGAVSIPFIGLLLDHTDTLSTLTILFTTSTAIGVFGLIPN
SFTWNLIGIVLLVVYRPFYYTVVSDYSSKVFGFDTFGTVYGLLSCICGIFNMSQNLLD
KWTHTTFNMNPFPINLTLVILTVVFSLTLTFYIRSQILPKPVNERGLSSNYQTI"
gene complement(680867..681538)
/gene="Fsh2"
/locus_tag="P301_M12726"
mRNA complement(680867..681538)
/gene="Fsh2"
/locus_tag="P301_M12726"
/product="Fsh2p"
CDS complement(680867..681538)
/gene="Fsh2"
/locus_tag="P301_M12726"
/note="Similar to YMR222C 'Putative serine hydrolase that
localizes to the cytoplasm; sequence is similar to S.
cerevisiae Fsh1p and Fsh3p and the human candidate tumor
suppressor OVCA2'"
/codon_start=1
/product="Fsh2p"
/protein_id="EWG89485.1"
/translation="MTKNVLMLHGLAQSGDYFASKTKGFRAEMEKLGYKLYYPTAPNE
FPPADVPDFLGEVIADAPGDGENTGVLAWLENDPSTGGYFIPQTTIDYLHNYVLENGP
FAGIVGFSQGAGVAGYLATDFNGLLGLTTEEQPPLEFFMAVSGFRFQPQQYQEQYDLH
PISVPSLHVQGELDTITEPAKVQGLYNSCTEDSRTLLMHSGGHFVPNSRGFVRKVAQW
LQQLA"
gene 681944..683359
/gene="Ubp8"
/locus_tag="P301_M12731"
mRNA 681944..683359
/gene="Ubp8"
/locus_tag="P301_M12731"
/product="Ubp8p"
CDS 681944..683359
/gene="Ubp8"
/locus_tag="P301_M12731"
/note="Similar to YMR223W 'Ubiquitin-specific protease
that is a component of the SAGA
(Spt-Ada-Gcn5-Acetyltransferase) acetylation complex;
required for SAGA-mediated deubiquitination of histone
H2B'"
/codon_start=1
/product="Ubp8p"
/protein_id="EWG89486.1"
/translation="MSICPHIQQVFQNEKSKDGVLKTCNAARYILNHSVPKEKFLNTM
KCGTCHEINSGATFMCLQCGFCGCWNHSHFLSHSKQIGHIFGINSNNGLLFCFKCEDY
IGNIDLINDAILAKYWDDVCTKTMVPSMERRDGLSGLINMGSTCFMSSILQCLIHNPY
FIRHSMSQIHSNNCKVRSPDKCFSCALDKIVHELYGALNTKQASSSSTSTNRQTGFIY
LLTCAWKINQNLAGYSQQDAHEFWQFIINQIHQSYVLDLPNAKEVSRANNKQCECIVH
TVFEGSLESSIVCPGCQNNSKTTIDPFLDLSLDIKDKKKLYECLDSFHKKEQLKDFNY
HCGECNSTQDAIKQLGIHKLPSVLVLQLKRFEHLLNGSNRKLDDFIEFPTYLNMKNYC
STKEKDKHSENGKVPDIIYELIGIVSHKGTVNEGHYIAFCKISGGQWFKFNDSMVSSI
SQAEVLKEQAYLLFYTIRQVN"
gene complement(683803..685881)
/gene="Mre11"
/locus_tag="P301_M12736"
mRNA complement(683803..685881)
/gene="Mre11"
/locus_tag="P301_M12736"
/product="Mre11p"
CDS complement(683803..685881)
/gene="Mre11"
/locus_tag="P301_M12736"
/note="Similar to YMR224C 'Subunit of a complex with
Rad50p and Xrs2p (MRX complex) that functions in repair of
DNA double-strand breaks and in telomere stability,
exhibits nuclease activity that appears to be required for
MRX function; widely conserved'"
/codon_start=1
/product="Mre11p"
/protein_id="EWG89487.1"
/translation="MDYPDPDTIRILITTDNHVGYNENDPITGDDSWKTFHEVMMLAK
NNNVDMVVQSGDLFHVNKPSKKSLYQVLKTLRLCCMGDKPCELELLSDPSQVFHYDEF
TNVNYEDPNFNISIPVFGISGNHDDASGDSLLCPMDILHATGLINHFGKVIESDKIKV
VPLLFQKGSTKLALYGLAAVRDERLFRTFKDGGVTFEVPTMREGEWFNLMCVHQNHTG
HTNTAFLPEQFLPDFLDMVIWGHEHECIPNLVHNPIKNFDVLQPGSSVATSLCEAEAQ
PKYVFILDIKYGEAPKMTPIPLETIRTFKMKSISLQDVPHLRPHDKDATSKYLIEQVE
EMIRDANEETKQKLADDGEGDMVAELPKPLIRLRVDYSAPSNTQSSIDYQVENPRRFS
NRFVGRVANGNNVVQFYKKRSPVTRSKKSGINGTSISDRDVEKLFSESGGELEVQTLV
NDLLNKMQLSLLPEVGLNEAVKKFVDKDEKTALKEFISHEISNEVGILSTNEEFLRTD
DAEEMKALIKQVKRANSVRPTPPKENDETNFAFNGNGLDSFRSSNREVRTGSSDITQS
HVDNESRITHISQAESSKPTSKPKRVRTATKKKIPAFSDSTVISDAENELGDNNDAQD
DVDIDEDDIIMVSTDEEDASYGLLNGRKTKTKTRSAASTKTASRRGKGRASRTPKTDI
LGSLLAKKRK"
gene complement(686188..686631)
/gene="Mrpl44"
/locus_tag="P301_M12746"
mRNA complement(join(686188..686426,686574..686631))
/gene="Mrpl44"
/locus_tag="P301_M12746"
/product="Mrpl44p"
CDS complement(join(686188..686426,686574..686631))
/gene="Mrpl44"
/locus_tag="P301_M12746"
/note="Similar to YMR225C Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl44p"
/protein_id="EWG89488.1"
/translation="MITKYFSKVIVRFNPFGKEAKVARLVLAAIPPTQRNMGTQIQSE
IISDYNKVKPLVKVTYKDKKEMEVDPSNMNFQELANHFDRHSKQLDLKHMLEMH"
gene complement(686821..687624)
/gene="Tma29"
/locus_tag="P301_M12751"
mRNA complement(686821..687624)
/gene="Tma29"
/locus_tag="P301_M12751"
/product="Tma29p"
CDS complement(686821..687624)
/gene="Tma29"
/locus_tag="P301_M12751"
/note="Similar to YMR226C 'NADP(+)-dependent
dehydrogenase; acts on serine, L-allo-threonine, and other
3-hydroxy acids; green fluorescent protein fusion protein
localizes to the cytoplasm and nucleus; may interact with
ribosomes, based on co-purification experiments'"
/codon_start=1
/product="Tma29p"
/protein_id="EWG89489.1"
/translation="MSQGRKAAERLAKKTVLITGASAGIGKATALEYLEASNGDMKLI
LAARRLEKLEELKKTIDQEFPNAKVHVAQLDITQAEKIKPFIENLPQEFKDIDILVNN
AGKALGSDRVGQIATEDIQDVFDTNVTALINITQAVLPIFQAKNSGDIVNLGSIAGRD
AYPTGSIYCASKFAVGAFTDSLRKELINTKIRVILIAPGLVETEFSLVRYRGNEEQAK
NVYKDTTPLMADDVADLIVYATSRKQNTVIADTLIFPTNQASPHHIFRG"
gene complement(687841..689613)
/gene="Taf7"
/locus_tag="P301_M12756"
mRNA complement(687841..689613)
/gene="Taf7"
/locus_tag="P301_M12756"
/product="Taf7p"
CDS complement(687841..689613)
/gene="Taf7"
/locus_tag="P301_M12756"
/note="Similar to YMR227C TFIID subunit (67 kDa), involved
in RNA polymerase II transcription initiation"
/codon_start=1
/product="Taf7p"
/protein_id="EWG89490.1"
/translation="MAVIRIKKPRGPGEKDQPLEGEPKLKRIRIKTKVTDEDIKPKPK
LKINLKKKKESADGKEKKNSLKLKLNLKKNEEPVKKIHKAPKLRLKPIRIPGEAYDSE
ASDIEDDPLIESGVILRILPDIQLEFVKNSLESGDYSGISIKWKNERHAVVTINDVMY
GAILVDLPTVIEVNKSVDRKNLLKTFDVSQMLLCIRPIQEEEEVYALEAPDTEDLVVK
HFEDIEDEIWENKETFLKGYNGAPLSDMEAKHLKEIALKGYDYKHGISPPLYNVRNRR
FRRKMDPNEIDYVEKVVDMLLKQDKQAEEVSYDLVDKSELQARQERVSSWENFKEEPG
EPLSRPALKKEEIHTIASAVGKQGAEEEGEEGMEEEEEEDLDLGAAFESEEEGSGAEG
DKEQQQEEVGDEVDQDTGGEDDDDDDDGDIEAAGGESESDDEKDENRQHTELLADELN
ELETTLAHTKHKLSKATNPLLKSRFIDSIKKLEKEAELKRKQLQQTEDSVQKQHQHRS
DAETANNVEEEEEEEEEEEEEDEVDEDEEDDEENDEDEDNVHEREHIQENKVVRELDE
APAEETLDQNDLDMMMLFGAEGDE"
gene 689854..690879
/gene="Mtf1"
/locus_tag="P301_M12761"
mRNA 689854..690879
/gene="Mtf1"
/locus_tag="P301_M12761"
/product="Mtf1p"
CDS 689854..690879
/gene="Mtf1"
/locus_tag="P301_M12761"
/note="Similar to YMR228W Mitochondrial RNA polymerase
specificity factor with structural similarity to
S-adenosylmethionine-dependent methyltransferases and
functional similarity to bacterial sigma-factors,
interacts with mitochondrial core polymerase Rpo41p"
/codon_start=1
/product="Mtf1p"
/protein_id="EWG89491.1"
/translation="MSVPIPGIKDISKLKFFYGFKYLWNPTVYNKIFDKLDLTKTYKH
PEELKVLDLYPGVGIQSAIFYNKYCPRQYSLLEKRSSLYKFLNAKFEGSPLQILKRDP
YDWSTYSNLIDEERIFVPEVQSSDHINDKFLTVAXXXXXXXXXXXXQWLSCIGNKNWL
YRFGKVKMLLWMPSTTARKLLARPGMHSRSKCSVVREAFTDTKLIAISDANELKGFDS
QCIEEWDPILFSAAEIWPTKGKPIALVEMDPIDFDFDVDNWDYVTRHLMILKRTPLNT
VMDSLGHGGQQYFNSRITDKDLLKKCPIDLTNDEFIYLTKLFMEWPFKPDILMDFVDM
YQTEHSG"
assembly_gap 690263..690295
/estimated_length=33
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(691162..696351)
/gene="Rrp5"
/locus_tag="P301_M12766"
mRNA complement(691162..696351)
/gene="Rrp5"
/locus_tag="P301_M12766"
/product="Rrp5p"
CDS complement(691162..696351)
/gene="Rrp5"
/locus_tag="P301_M12766"
/note="Similar to YMR229C 'RNA binding protein with
preference for single stranded tracts of U's involved in
synthesis of both 18S and 5.8S rRNAs; component of both
the ribosomal small subunit (SSU) processosome and the 90S
preribosome'"
/codon_start=1
/product="Rrp5p"
/protein_id="EWG89492.1"
/translation="MVASTKRKRDEDFPLSREDSTKQPSTSSLVRNTEEVSFPRGGAS
ALTPLELKQVANEAASDVLFGNESVKASEPASRPLKKKKTTKKSTSKDSEASSANSDE
ARAGLIEHVNFKTLKNGSSLLGQISAITKQDLCITFTDGISGYVNLTHISEEFTSILE
DLDEDMDSDTDAADEKKSKVEDAEYESSDDEDEKLDKSNELPNLRRYFHIGQWLRCSV
IKNTSLEPSTKKSKKKRIELTIEPSSVNTYADEDLVKSTSIQCAVKSIEDHGATLDVG
LPGFTGFIAKKDFGNFEKLLPGAVFLGNITKKSDRSIVVNTDFSDKKNKITQISSIDA
IIPGQIVDLLCESITKNGIAGKVFGLVSGVVNVSHLRTFSEEDLKHKFAIGSSIRCRI
IACLENKSGDKVLILSNLPHILKLEDALRSTEGLDAFPIGYTFESCSIKGRDSEYLYL
ALDDDRLGKVHSSRVGEIENSENLSSRVLGYSPVDDIYQLSTDPKYLKLKYLRTNDIP
IGELLPSCEITSVSSSGIELKIFNGQFKASVPPLHISDTRLVYPERKFKIGSKVKGRV
ISVNSRGNVHVTLKKSLVNIEDNELPLVSTYENAKNIKEKNEKTLATVQVFKPNGCII
SFFGGLSGFLPNSEISEVFVKRPEEHLRLGQTVIVKLLDVDADRRRIIATCKVSNEQA
AQQKDTIENIVPGRTIITVHVIEKTKDSVIVEIPDVGLRGVIYVGHLSDSRIEQNRAQ
LKKLRIGTELTGLVIDKDTRTRVFNMSLKSSLIKDAKKETLPLTYDDVKDLNKDVPMH
AYIKSISDKGLFVAFNGKFIGLVLPSYAVDSRDIDISKAFYINQSVTVYLLRTDDKNQ
KFLLSLKAPKVKEEKKKVESNIEDPVDSSIKSWDDLSIGSIVKAKIKSVKKNQLNVIL
AANLHGRVDIAEVFDTYEEITDKKQPLSNYKKDDVIKVKIIGNHDVKSHKFLPITHKI
SKASVLELSMKPSELKSKEVHTKSLEEINIGQELTGFVNNSSGNHLWLTISPVLKARI
SLLDLADNDSNFSENIESVFPLGSALQVKVASIDREHGFVNAIGKSHVDINMSTIKVD
DELPGRVLKIAEKYVLLDLGNKVTGISFITDALNDFSLTLKEAFEDKINNVIPTTVLS
VDEQNKKIELSLRSATAKTRSIKSHEDLKQGEIVEGIVKNVNDKGIFVYLSRKVEAFV
PVSKLSDSYLKEWKKFYKPMQYVLGKVVTCDEDSRISLTLRESEINGDLKVLKTYSDI
KAGEVFEGTIKNVTDFGVFVKLDNTVNVTGLAHITEIADKKPEDLSALFGVGDRVKAI
VLKTNPEKKQISLSLKASHFSKEAELASTTTTTTTADQLEKEDEDEVMADAGFNDSDS
ESDIGDQNTEVADRKPETSSDGLSLSAGFDWTASILDQAQEEEESDQDQEDFTENKKH
KHKRRKENVVQDKTIDINTRAPESVADFERLLIGNPNSSVVWMNYMAFQLQLSEIEKA
RELAERALKTINFREEAEKLNIWIAMLNLENTFGTEETLEEVFSRACQYMDSYTIHTK
LLGIYEISEKFDKAAELFKATAKKFGGEKVSIWVSWGDFLISHNEEQEARTILGNALK
ALPKRNHIEVVRKFAQLEFAKGDPERGRSLFEGLVADAPKRIDLWNVYVDQEVKAKDK
KKVEDLFERIITKKITRKQAKFFFNKWLQFEESEGDEKTIEYVKAKATEYVASHESQK
ADE"
gene 697642..698369
/gene="Rps10b"
/locus_tag="P301_M12781"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 698449..698551
/estimated_length=103
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene <698552..698691
/locus_tag="P301_M12786"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 698780..701869
/gene="Pep5"
/locus_tag="P301_M12791"
mRNA 698780..701869
/gene="Pep5"
/locus_tag="P301_M12791"
/product="Pep5p"
CDS 698780..701869
/gene="Pep5"
/locus_tag="P301_M12791"
/note="Similar to YMR231W 'Component of CORVET tethering
complex; peripheral vacuolar membrane protein required for
protein trafficking and vacuole biogenesis; interacts with
Pep7p'"
/codon_start=1
/product="Pep5p"
/protein_id="EWG89493.1"
/translation="MSLSSWRQFQLFENIPIRDPNFGGDSLLYSDPTLCAATIVDPQT
LIIAVNSNIIKVVKLNQSQVIHEFQSFPHDFQITFLKVINGEFLVALAESIGKPSLIR
VYKLEKLPNREQLYHSQVELKNGNNTYPISVVSISNDLSCIVVGFINGKIILIRGDIS
RDRGSQQRIIYEDPSKEPITALFLNNDATACFAATTSRIFLFNTTGRNRGRPSLVLNS
KNGLDLNCGSFNPATNEFICCLSNFIEFFSSSGKKHQFAFDLSLRKRIFCVDKDHILI
VTEETGAPTTSISVNELSPTIINRIFIIDAKNKIISLNFVVSSAIIDIFSTSQSGKNI
TYLLTSEGVMHRITPKSLENQINIIIQKELYPFALQLAKQHSLSPLDVQEIHKKYGDY
LFKKGLRKEATDQYIQCLDVVETSEIISKFGVKEVPDPESMRNLADYLWSLIKNSISQ
RDHVTLLLIVLIKLKDVEGIDTFIQHFDRKGIWNEGVVMDDMDDVTFFYSDNDFFDLD
LILELMKESDFKRLSYRLAKKYSKDSLIIVDILLNLLHNPVKAIKYIKSLPIDETLRC
LVTYSKKLLEESPNETNALLIEVFTGKFKPSTFEVDLDRRDTTGDFSENIRTVFYSYK
TFFNYMNSNGTSDAMSESSEASHEHEEPTYHPPKPSIVFSSFVTKPFEFVVFLEACLA
CYQQYEGFDEDRQVILTTLYDLYLNLAQNDVPERIDDWRSRATGVLRESNKLVYSAAS
NNTSKRVDNSIMLLISHMDQSSASAKDKTKIDIASFANDNPEMDLLSTFRAMTLNEEP
STCLKFLEKYGTEEPKLLQVALSYFVSNKLIFKEMGGNEVLKEKVLRPIIEGERMPLL
DIIKALSRTNVAHFGLIQDIIIDHVKTEDTEIKRNEKLIESYDKELKEKNKKLKNTIN
SDQPLHVPLKNQTCFMCRLTLDIPVVFFKCGHIYHQHCLNEEEDTLESERKLFKCPKC
LVDLETSNKLFEAQHEVVEKNDLLNFALNSEEGSRDRFKVITEFLGRGAISYSDITI"
gene 702161..704194
/gene="Fus2"
/locus_tag="P301_M12796"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 703330..703544
/estimated_length=215
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 704420..705100
/gene="Tri1"
/locus_tag="P301_M12801"
mRNA 704420..705100
/gene="Tri1"
/locus_tag="P301_M12801"
/product="Tri1p"
CDS 704420..705100
/gene="Tri1"
/locus_tag="P301_M12801"
/note="Similar to YMR233W 'Non-essential sumoylated
protein of unknown function with similarity to components
of human SWI/SNF complex including SMRD3; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm, nucleus and nucleolus'"
/codon_start=1
/product="Tri1p"
/protein_id="EWG89494.1"
/translation="MADINKYIPMVDAILSVSNPDEISPKRVRKALQILYSVNLDSQR
KLINELILERFGDIQENPRVLIPKNDLISRDQELSLRLQKEEERPLRSTRKRKGKSES
KSKRKKKKNDSPDSNSISVRKVLLSAPLQKFLGSEELPRTQVVKMIWQYIKEHDLQNP
KDRREILCDEKMEPIFGKKMTMFSMNKLLTKHLFNPDEIVKHEEEQKQTPEKEIKLEN
ESLPNLSG"
gene 705500..706546
/gene="Rnh1"
/locus_tag="P301_M12806"
mRNA 705500..706546
/gene="Rnh1"
/locus_tag="P301_M12806"
/product="Rnh1p"
CDS 705500..706546
/gene="Rnh1"
/locus_tag="P301_M12806"
/note="Similar to YMR234W 'Ribonuclease H1; able to bind
double-stranded RNAs and RNA-DNA hybrids; associates with
RNAse polymerase I; the homolog of mammalian RNAse HII
(the S. cerevisiae homolog of mammalian RNAse HI is
RNH201)'"
/codon_start=1
/product="Rnh1p"
/protein_id="EWG89495.1"
/translation="MARQGNFYAVRKGRETGIYNTWNECKNQVDGYGGAIYKKFNSYE
QAKSFLGQPNTTSNYGSSTHAGGQVSKPHTTQKRVHRRNRPLHYSSLTSSSACSSLSS
ANTNTFYSVKSNVPNIESKIFNNWKDCQAYVKHKRGITFKKFEDQLAAENFISGMSAH
DYKLMNISKESFESKYKLSSNTMYNKSMNVYCDGSSFGNGTSSSRAGYGAYFEGAPEE
NISEPLLSGAQTNNRAEIEAVSEALKKIWEKLTNEKEKVNYQIKTDSEYVTKLLNDRY
MTYDNKKLEGLPNSDLIVPLVQRFVKVKKYYELNKECFKNNGKFQIEWVKGHDGDPGN
EMADFLAKKGASRR"
gene complement(706746..707969)
/gene="Rna1"
/locus_tag="P301_M12811"
mRNA complement(706746..707969)
/gene="Rna1"
/locus_tag="P301_M12811"
/product="Rna1p"
CDS complement(706746..707969)
/gene="Rna1"
/locus_tag="P301_M12811"
/note="Similar to YMR235C GTPase activating protein (GAP)
for Gsp1p, involved in nuclear transport"
/codon_start=1
/product="Rna1p"
/protein_id="EWG89496.1"
/translation="MATLHFVPQHEEEQVYSISGKALKLTTSDDIKPYLEELAALKTC
TKLDLSGNTIGTEASEALAKCIAENTQVRESLVEVNFADLYTSRLVDEVVDSLKFLLP
VLLKCPHLEIVNLSDNAFGLRTIELLEDYIAHAVNIKHLILSNNGMGPFAGERIGKAL
FHLAQNKKAASKPFLETFICGRNRLENGSAVYLALGLKSHSEGLKVVKLYQNGIRPKG
VATLIHYGLQYLKNLEILDLQDNTFTKHASLILAKALPTWKDSLFELNLNDCLLKTAG
SDEVFKVFTEVKFPNLHVLKFEYNEMAQETIEVSFLPAMEKGNLPELEKLEINGNRLD
EDSDALDLLQSKFDDLEVDDFEEVDSEDEEGEDEEDEDEDEKLEEIETERLEKELLEV
QVDDLAERLAETEIK"
gene 708205..708678
/gene="Taf9"
/locus_tag="P301_M12821"
mRNA 708205..708678
/gene="Taf9"
/locus_tag="P301_M12821"
/product="Taf9p"
CDS 708205..708678
/gene="Taf9"
/locus_tag="P301_M12821"
/note="Similar to YMR236W Subunit (17 kDa) of TFIID and
SAGA complexes, involved in RNA polymerase II
transcription initiation and in chromatin modification,
similar to histone H3"
/codon_start=1
/product="Taf9p"
/protein_id="EWG89497.1"
/translation="MNGGGKNVLNKNSVGSVSEVGPDSTQEETPRDVRLLHLLLASQS
IHQYEDQVPLQLMDFAHRYTQGVLKDALVYNDYAGSGNSAGSGLGVEDIRLAIAARTQ
YQFKPTAPKELMLQLAAERNKKALPQVMGTWGVRLPPEKYCLTAKEWDLEDPKSM"
gene 708983..711158
/gene="Bch1"
/locus_tag="P301_M12826"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 711588..712964
/gene="Dfg5"
/locus_tag="P301_M12831"
mRNA 711588..712964
/gene="Dfg5"
/locus_tag="P301_M12831"
/product="Dfg5p"
CDS 711588..712964
/gene="Dfg5"
/locus_tag="P301_M12831"
/note="Similar to YMR238W Putative mannosidase, essential
glycosylphosphatidylinositol (GPI)-anchored membrane
protein required for cell wall biogenesis in bud
formation, involved in filamentous growth, homologous to
Dcw1p"
/codon_start=1
/product="Dfg5p"
/protein_id="EWG89498.1"
/translation="MIVNISAKMILSICFTFLSFFKATHAMDLDTTSKTSICDATALI
QGGMLDYYEGTRYGGTVGMFQSPYYWWHAGEAFGGMLENWFLCENDTYQELLYDALLA
QTGSNYDYIPSNQTMVEGNDDQGIWGITVMGAVERNFTDPGDGKPGWLAMVQAVFNTM
YSRWDSEHCGGGLRWQIFTWNSGYNYKNTVSNACLFQIAARLGRYTGNTTYLEVAEQV
FDWLVDVGYVVLNDTANVFDGAEIDTNCTDITKIEWTYNHGIVLGGLAYMYNATNGTG
EWETSLTKILNGAKSYFFKDSIMYESACQDYGTCNTDQRTFKSIFSRMLGLTSVMAPF
TRDTIDDLIKTSAEAAAKSCNGGTDGHTCGLNWQKQTNDGYYGLGEQMSALEVIQNLL
IHDRPAPYKEDNGGTSKGDANAGMNSSTTNVLQNNLNIKKGDRAGAAIITAVILSVLT
GGAVWMLF"
gene complement(713127..713198)
/locus_tag="P301_M12836"
tRNA complement(713127..713198)
/locus_tag="P301_M12836"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg3);
TR(UCU)M1"
assembly_gap 713268..713373
/estimated_length=106
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(713497..714912)
/gene="Rnt1"
/locus_tag="P301_M12841"
mRNA complement(713497..714912)
/gene="Rnt1"
/locus_tag="P301_M12841"
/product="Rnt1p"
CDS complement(713497..714912)
/gene="Rnt1"
/locus_tag="P301_M12841"
/note="Similar to YMR239C 'RNAase III; involved in rDNA
transcription and rRNA processing; also cleaves a
stem-loop structure at the 3' end of U2 snRNA to ensure
formation of the correct U2 3' end; involved in
polyadenylation-independent transcription termination'"
/codon_start=1
/product="Rnt1p"
/protein_id="EWG89499.1"
/translation="MGSKVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXNEFDTEQKQPILQEISKADMEKLEKLEQVKREKREK
IDVNVYENLNEKEDEEEDEGEDSYDPTKAGDIVKATKWPPKLPEIQDLAIRARVFIHK
STIKDKVYLSGSEMINAHNERLEFLGDSILNSVMTLIIYNKFPDYSEGQLSTLRMNLV
SNEQIKQWSIMYNFHEKLKTNFDLKDENSNFQNGKLKLYADVFEAYIGGLMEDDPRNN
LPKIRKWLRKLAKPVIEEATRNQVALEKTDKLDMNAKRQLYSLIGYASLRLHYVTVKK
PTAVDPNSIVECRVGDGTVLGTGVGRNIKIAGIRAAENALRDKKMLDFYAKQRAAIPR
SESVLKDPSQKNKKRKFSDTS"
assembly_gap 714543..714896
/estimated_length=354
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(715165..716475)
/gene="Cus1"
/locus_tag="P301_M12846"
mRNA complement(715165..716475)
/gene="Cus1"
/locus_tag="P301_M12846"
/product="Cus1p"
CDS complement(715165..716475)
/gene="Cus1"
/locus_tag="P301_M12846"
/note="Similar to YMR240C Protein required for assembly of
U2 snRNP into the spliceosome, forms a complex with Hsh49p
and Hsh155p"
/codon_start=1
/product="Cus1p"
/protein_id="EWG89500.1"
/translation="MARTKSRKRSGNNQNKNASVVNNKAEIAAMIDARRLEQKKKGGV
TNSKGKTNKVVDAKLEKEFKDVLQRFQVQENDTPKEITKDEKNNHVVIVEKNPVMNRK
HTAEDELEDTPSDGIEEHLSARKRRKTEKPSLSQLKSQVPYPQIIEWYDCDARYPGLL
ASIKCTKNVIPVPSHWQSKKEYLSGRSLLGKRPFELPDIIKKTNIEQMRSTLPQSGLD
GQDEKSLKEASRARVQPKMGALDLDYKKLHDVFFKIGANWKPDHLLCFGDVYYENRNL
FEETNWKRMVDHKRPGRISQELRAIMNLPEGQLPPWCMKMKDIGLPTGYPDLKIAGLN
WDITNLKGDVYGKIIPNHHSRSKKQGRNYFGALISFETPEFENSKEDTQANAENGRQD
DKIDDEVEHKLDHFQEDISEVTSAEEKLERNEEESEKQLYTVLK"
gene 717192..718136
/gene="Yhm2"
/locus_tag="P301_M12851"
mRNA 717192..718136
/gene="Yhm2"
/locus_tag="P301_M12851"
/product="Yhm2p"
CDS 717192..718136
/gene="Yhm2"
/locus_tag="P301_M12851"
/note="Similar to YMR241W 'Carrier protein that exports
citrate from and imports oxoglutarate into the
mitochondrion, causing net export of NADPH reducing
equivalents; also associates with mt nucleoids and has a
role in replication and segregation of the mt genome'"
/codon_start=1
/product="Yhm2p"
/protein_id="EWG89501.1"
/translation="MPSTTNTAAANVIEKKPVSFSNILLGACLNLSEVTTLGQPLEVV
KTTMAANRNFTFLESVKHVWSRGGILGYYQGLIPWAWIEASTKGAVLLFVSAEAEYRF
KSLGLNNFASGILGGVTGGVTQAYLTMGFCTCMKTVEITRHKSASAGGVPQSSWSVFK
NIYKKEGIRGINKGVNAVAIRQMTNWGSRFGLSRLVEDGIRKITGKTNKDDKLNPFEK
IGASALGGGLSAWNQPIEVIRVEMQSKKEDPNRPKNLTVGKTFKYIYQSNGLKGLYRG
VTPRIGLGIWQTVFMVGFGDMAKEFVARMTGETPVAKH"
gene complement(718454..719449)
/gene="Rpl20a"
/locus_tag="P301_M12856"
mRNA complement(join(718454..718971,719449))
/gene="Rpl20a"
/locus_tag="P301_M12856"
/product="Rpl20ap"
CDS complement(join(718454..718971,719449))
/gene="Rpl20a"
/locus_tag="P301_M12856"
/note="Similar to YMR242C Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl20Bp and
has similarity to rat L18a ribosomal protein"
/codon_start=1
/product="Rpl20ap"
/protein_id="EWG89502.1"
/translation="MAHFKEYQVIGRRLPTESVPEPKLFRMRIFASNEVIAKSRYWYF
LQKLHKVKKASGEIVSINQINEAHPTKVKNFGVWVRYDSRSGTHNMYKEIRDVSRVAA
VETLYQDMAARHRARFRSIHILKVAEIEKTADVKRQYVKQFLTKDLKFPLPHRVQKST
KTFSYKRPSTFY"
gene complement(720068..721396)
/gene="Zrc1"
/locus_tag="P301_M12866"
mRNA complement(720068..721396)
/gene="Zrc1"
/locus_tag="P301_M12866"
/product="Zrc1p"
CDS complement(720068..721396)
/gene="Zrc1"
/locus_tag="P301_M12866"
/note="Similar to YMR243C 'Vacuolar membrane zinc
transporter, transports zinc from the cytosol into the
vacuole for storage; also has a role in resistance to zinc
shock resulting from a sudden influx of zinc into the
cytoplasm'"
/codon_start=1
/product="Zrc1p"
/protein_id="EWG89503.1"
/translation="MITGKELRIISLLTLDTVFFLLEITIGYMSHSLALIADSFHMLN
DIISLLVALWAVDVAKNRGPDAKYTYGWKRAEILGALINAVFLIALCFSIMIEALQRL
IEPQEIQNPRLVLYVGVAGLISNVVGLFLFHDHGSDSLHSHSHGSVESGNNDLDIESN
ATHSHSHASLPNDNLAIDEDAISSPGPSGQIGEVLPQSVVNRLSNESQPLLNHDDHDH
SHESKKPGHRSLNMHGVFLHVLGDALGNIGVIAAALFIWKTEYSWRYYSDPIVSLIIT
IIIFSSALPLSRRASRILLQATPSTISADQIQREILAVPGVIAVHDFHVWNLTESIYI
ASIHVQIDCAPDKFMSSAKLIRKIFHQHGIHSATVQPEFVSGDVNEDIRRRFSIIAGG
SPSSSQEAFDSHGNTEHGRKKRSPTAYGATTASSNCIVDDAVNCNTSNCL"
gene 722479..723546
/locus_tag="P301_M12871"
mRNA 722479..723546
/locus_tag="P301_M12871"
/product="hypothetical protein"
CDS 722479..723546
/locus_tag="P301_M12871"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89504.1"
/translation="MVLCKLLTPYSLLSILSVGVFTATAAPSPSIQMTENTNQDHHEH
AKRGGTCAFPNYDGMVAVQKGGSNGGWAMSPDQECSYGSWCPYACKPGQLMGQWDPSA
TTYSYPKCQNGGLYCDSNGNLQKPNSDKDYCYDGKGTVIAKNNANSGDVAFCQTVLPG
NEAMLIPTLVGSGSKQTLAVPGTDYWASTASHYYVNAPGVSVEDACQWGSSANPQGNW
APFVAGSNMDDNQNTFVKIGWNPVYLESSCPFKNVKPSFGIRITCDDESQCEGLPCSI
DPSSNGVNEVTSSGGGSSGAGGGNFCVVTARNGAKANIEVFDVGSGSSSKGKRELNPL
DVITTTVTETKYKTVTVTAKT"
gene complement(723746..724060)
/locus_tag="P301_M12876"
mRNA complement(723746..724060)
/locus_tag="P301_M12876"
/product="hypothetical protein"
CDS complement(723746..724060)
/locus_tag="P301_M12876"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89505.1"
/translation="MGLFSFDGGKKESQPPNTRSQRKLCWESRDAFFQCLDKADILDA
MDPKNSKSIKSHCKVENEKFEENCAHSWIKYFKEKRVIDFKREETIKRIEQEAKQRER
NQ"
gene 725033..727117
/gene="Faa4"
/locus_tag="P301_M12886"
mRNA 725033..727117
/gene="Faa4"
/locus_tag="P301_M12886"
/product="Faa4p"
CDS 725033..727117
/gene="Faa4"
/locus_tag="P301_M12886"
/note="Similar to YMR246W 'Long chain fatty acyl-CoA
synthetase, activates imported fatty acids with a
preference for C12:0-C16:0 chain lengths; functions in
long chain fatty acid import; important for survival
during stationary phase; localized to lipid particles'"
/codon_start=1
/product="Faa4p"
/protein_id="EWG89506.1"
/translation="MTEQYSVAVGEAANEHETAPRRNIRVKDQPLIRPINSSASTLYE
FALECFTKGGKRDGMAWRDIIDIHETKKTIVKRVDGKDKPIEKTWLYYELTPYITMTY
EEMICVMHDIGRGLIKIGVKPNGENKFHIFASTSHKWMKTFLGCMSQGIPVVTAYDTL
GESGLIHSMVETDSVAIFTDNQLLSKLAVPLKTAKNVKFVIHNEPIDPSDKRQNGKLY
KAAKDAVDKIKEVRPDIKIYSFDEIIEIGKKAKDEVELHFPKPEDPACIMYTSGSTGT
PKGVVLTHYNIVAGIGGVGHNVIGWIGPTDRIIAFLPLAHIFELTFEFEAFYWNGILG
YANVKTLTPTSTRNCQGDLMEFKPTVMVGVAAVWETVRKGILAKINELPGWSQTLFWT
VYALKERNIPCSGLLSGLIFKRIREATGGNLRFILNGGSAISIDAQKFLSNLLCPMLI
GYGLTEGVANACVLEPEHFDYGIAGDLVGTITAKLVDVEDLGYFAKNNQGELLLKGAP
ICSEYYKNPEETAAAFTDDGWFRTGDIAEWTPKGQVKIIDRKKNLVKTLNGEYIALEK
LESIYRSNPYVQNICVYADENKVKPVGIVVPNLGHLSKLAIELGIMVPGEDVESYIHE
KKLQDAVCKDMLSTAKSQGLNGIELLCGIVFFEEEWTPENGLVTSAQKLKRRDILAAV
KPDVERVYKENT"
gene complement(727336..728338)
/locus_tag="P301_M12891"
ncRNA complement(727336..728338)
/ncRNA_class="snoRNA"
/locus_tag="P301_M12891"
/product="SNR86"
gene complement(728584..733243)
/gene="Rkr1"
/locus_tag="P301_M12901"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 729127..729640
/estimated_length=514
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 733628..734063
/estimated_length=436
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 734558..734701
/locus_tag="P301_M12911"
mRNA 734558..734701
/locus_tag="P301_M12911"
/product="hypothetical protein"
CDS 734558..734701
/locus_tag="P301_M12911"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89507.1"
/translation="MAHKCASAKLLSGIMALLFNGKSLLRPICLHVHNHLVSNSDTNI
VWP"
gene 736075..737832
/gene="Gad1"
/locus_tag="P301_M12916"
mRNA 736075..737832
/gene="Gad1"
/locus_tag="P301_M12916"
/product="Gad1p"
CDS 736075..737832
/gene="Gad1"
/locus_tag="P301_M12916"
/note="Similar to YMR250W 'Glutamate decarboxylase,
converts glutamate into gamma-aminobutyric acid (GABA)
during glutamate catabolism; involved in response to
oxidative stress'"
/codon_start=1
/product="Gad1p"
/protein_id="EWG89508.1"
/translation="MLHRHGSKQKNFENIAGKVVHDLAGLQLLSNDVQKSAVQSGHQG
SNNMRDTSSQGMANKYSVPKKGLPADLSYQLIHNELTLDGNPHLNLASFVNTFTTDQA
RKLIDENLTKNLADNDEYPQLIELTQRCISMLAQLWHANPDEEPIGCATTGSSEAIML
GGLAMKKRWEHRMKNAGKDASKPNIIMSSACQVALEKFTRYFEVECRLVPVSHRSHHM
LDPESLWDYVDENTIGCFVILGTTYTGHLENVEKVADVLSQIEAKHPDWSNTDIPIHA
DGASGGFIIPFGFEKEHMKAYGMERWGFNHPRVVSMNTSGHKFGLTTPGLGWVLWRDE
SLLADELRFKLKYLGGVEETFGLNFSRPGFQVVHQYFNFVSLGHSGYRTQFQNSLFVA
RAFSFELLNSSKLPGCFEIVSSIHESIENDSAPKSVKDYWEHPQAYKPGVPLVAFKLS
KKFHEEYPEVPQAILSSLLRGRGWIIPNYPLPKATDGSDEKEVLRVVFRSEMKLDLAQ
LLIVDIESILTKLIHSYEKVCHHIELASEQTPERKSSFIYEMLLALASPQDDIPTPDE
IEKKNKLKETTTRNYRGTC"
gene 738171..739271
/gene="Gto3"
/locus_tag="P301_M12926"
mRNA 738171..739271
/gene="Gto3"
/locus_tag="P301_M12926"
/product="Gto3p"
CDS 738171..739271
/gene="Gto3"
/locus_tag="P301_M12926"
/note="Similar to YMR251W 'Omega class glutathione
transferase; putative cytosolic localization'"
/codon_start=1
/product="Gto3p"
/protein_id="EWG89509.1"
/translation="MSEKSASNNKAEFKRQSSPFREIISADHPIYKPAKGRYWLYVAL
PCPWAQRTLITRALKGLAPIIGCSVAHWHLDDKGWRFLEEGDGKTNERHWFDIAGGIS
SVNLNTSTPVANIPNNAHRLLVDGTDEPHYGYKRLSDFYFKTKPDYKGRFTVPVLWDL
ETCTIVNNESSDIIRIMNSAAFDEFVGEEYRQVRLVPRSLEAQITEFNSWVYDKINNG
VYKAGFAECAEVYEREVTSLFQYLDKLENLLDKKYTDLEAEYGKNNKDKILDRYFAIG
DTLTEADVRLYPTIVRFDVVYHQHFKCNLATIRDDYSRIHTWLKNIYWRHEAFQRTTD
FTHIKLGYTRSQPRVNPIGITPLGPKPDIRPP"
gene 740006..740185
/gene="Hor7"
/locus_tag="P301_M12936"
mRNA 740006..740185
/gene="Hor7"
/locus_tag="P301_M12936"
/product="Hor7p"
CDS 740006..740185
/gene="Hor7"
/locus_tag="P301_M12936"
/note="Similar to YMR251W-A no_gene_description"
/codon_start=1
/product="Hor7p"
/protein_id="EWG89510.1"
/translation="MKLSQVVVSAVAFTGLVSAANSSNSSSSKNAAQPIAGLNNGKVA
GAAGVALAGALAFLI"
gene complement(740568..740972)
/locus_tag="P301_M12941"
mRNA complement(740568..740972)
/locus_tag="P301_M12941"
/product="hypothetical protein"
CDS complement(740568..740972)
/locus_tag="P301_M12941"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89511.1"
/translation="MFGKVFVSYIRTRIGFKPLSTIYTPVSSSSHSFDKEACFPFKKW
HELNMSQKQEFIQRFVKNYRHQYPSSKTNVSLKGLSIGMDEHNDSPSVFGIFYNDIWK
SFKNEQLGTNNDNMKSGSRFSHLSFKQXXXXX"
assembly_gap 740573..740584
/estimated_length=12
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(741198..742442)
/locus_tag="P301_M12946"
mRNA complement(741198..742442)
/locus_tag="P301_M12946"
/product="hypothetical protein"
CDS complement(741198..742442)
/locus_tag="P301_M12946"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89512.1"
/translation="MNPSVPKVMKRENNTHLLVSKEMNDTSLQLPSTTRSLSPKESNS
NEDFNVDGNETTLQRISKDYLKPNIGLVLLTVSYFFNSAMVVSTKVLENDPDDIANDR
QIKPLQILLVRMVITYIGTLIYMYINKSTISDVPFGKPEVRKWLVLRGCTGFFGVFGM
YYSLMYLTISDAVLITFLAPSLTIFLSWVILRERFTKVEALGSLISLLGVVLIVRPSF
LFGTPELTDSSSQIVESSDPKSRLIATLVGLWGVLGMSCVYIIIRYIGKRAHAIMSVS
YFSLITAIVSFIGINTIPSMKFQIPHSKKQWILFGNLGVSGFIFQLLLTMGIQRERAG
RGSLMTYTQLLYAVFWDVALYKHWPNIWSWIGMIIIISATLWVIRIRAANNETTAKDL
TPIIDDEENSIPLTEFDLSDSK"
gene 743252..743818
/gene="Gfd1"
/locus_tag="P301_M12966"
mRNA 743252..743818
/gene="Gfd1"
/locus_tag="P301_M12966"
/product="Gfd1p"
CDS 743252..743818
/gene="Gfd1"
/locus_tag="P301_M12966"
/note="Similar to YMR255W Coiled-coiled protein of unknown
function, identified as a high-copy suppressor of a dbp5
mutation"
/codon_start=1
/product="Gfd1p"
/protein_id="EWG89513.1"
/translation="MPLESIWADAPDEEPIKKQKPSHKRSNNNKKNNNSRWSNESSSN
NKKKDSVNKVKNNKGNHESKTKNKIKETLPREKKPPHSQGKISPVSESLAINPFSQKA
TEISPPPVSPSKMKTTKTQSKQDTASKMKLLKKKIEEQREILQKTHHKNQQQQVLMDF
LNDEGSSNWVDDDEEELILQRLKTSLKI"
assembly_gap 743931..744118
/estimated_length=188
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(744197..744379)
/gene="Cox7"
/locus_tag="P301_M12971"
mRNA complement(744197..744379)
/gene="Cox7"
/locus_tag="P301_M12971"
/product="Cox7p"
CDS complement(744197..744379)
/gene="Cox7"
/locus_tag="P301_M12971"
/note="Similar to YMR256C Subunit VII of cytochrome c
oxidase, which is the terminal member of the mitochondrial
inner membrane electron transport chain"
/codon_start=1
/product="Cox7p"
/protein_id="EWG89514.1"
/translation="MANKVIQLQKIFQSSTKPLWWRHPRSALYLYPFYAIFAVAVVTP
LLYIPNAIRGIKAKKA"
gene complement(744879..747211)
/gene="Pet111"
/locus_tag="P301_M12981"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 747276..747336
/estimated_length=61
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(747868..749529)
/locus_tag="P301_M12986"
mRNA complement(747868..749529)
/locus_tag="P301_M12986"
/product="hypothetical protein"
CDS complement(747868..749529)
/locus_tag="P301_M12986"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89515.1"
/translation="MAFQDQDIFIVFSHASLFLNQNDLLSLSLTSKKMHDMIAIPRLY
SNIHITKNPVLRTNKWFLDGGKTYVSGYRSVLKTGDKNDIFLYDRIERLLETSHLKCI
KQLTIDEDLFHNREEGLQLLQRLVNEITDLDVIESLDIKDPTLFELCSAKYYRLSSLK
KRVVYGETGFDGIKLWQNFKSLKWQLPESLDLQNVIIPEVGVMLMKQLNGGELEIKDE
AYSSLRVFEYFDSLNLRFKNLRRLKLNHVHKQGDGSATSMRLSSRAFKDVVNLSNLKA
LELEFSCEVDDCECDDDFLQDITGNLVSLTSLGFIEKTFTKKGYHYMDEKWDLVVNKF
ILNLPNVSKDLRLLSIRHDPPLNGKGIDTVDGNLLRRKKLYEKVLPKLTSLETIIAPT
VLQSITSYEMYACDLLWNGCKCAFCSKYLPLFDKYIMNHQYFSTPDTRYLDIIPIVFA
AYTGKSLAKRFDPQKNWDLDLLQYAPEDTTWNFHGFERIHHFASYECYFDESSFEPLA
TIISHFFYPYMNYLIKILPNLRQTMLSGIYFSVSPELHTYETIYD"
gene complement(749869..754131)
/locus_tag="P301_M12996"
mRNA complement(749869..754131)
/locus_tag="P301_M12996"
/product="hypothetical protein"
CDS complement(749869..754131)
/locus_tag="P301_M12996"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89516.1"
/translation="MTTDVQFSSQEIELFRVKEFLIANNPAKINNENKDAVLTQIEHD
FRYLIQYIKDGLPNLNESTRLIFPDTFSICLLRSHQIIASKKIDSQEFLSAVKEQLLT
EANANIIFEYVLDFWADGGAPLMNALRDLFSKLLNLLKITYPMSTLKDVLFNWMNEIL
EVPSTLRVQYYLIDALSSDFDLYYIIEKKPHFIDNSFSLMNSDLLANSVGKCIVSLLL
NIYEKHFKKNESFVQEWIQLWKSCALKYIHDKQYTKSINLYIMIPLFKNMPNSAFTLF
LECMSNKDPSLLLSLLKIGQELGIEEEPFCDNKYTTVDSVNKLIEQDEYKLQVFEILT
FSTKKSKPIRPFVFKTIKQYLYVFFVDTELERRNYFCSSMKHFIFRTRDCAYSLARDA
RKLKKAEKFPDEQREKLAQVEEARAFLVWLCNFIKYNLAPGTLYQANVTSLKLMHILI
KSGVDKSTPQKFLDNQNKREYPFSIPILQDVTFLRLLIDLLVSNYADVRELSKEMLFI
MISADESRGLFLDTLDANALKWTATSLLSDYEKGDAGATVYEFIFTVMGSQRSFIDQT
IDILAQMVQNLQNDSIGCAENSIGPHFAALSLILNKFNSEENHQDTSKIISKLINLVL
KSWEATRNVVCHDSAHGILPEKYANCGVPDQVIISHAFRAIKEASYLLETILKKYPLT
RDQLDSIGDLFIVQLSTIRHSGAFQAVLPGLKAFCIRCQLEFPAILEELLSKSVKSLK
SKTQHITRRSGGLPFLVTTVLSAEVTKGRPLLQKTFENLLLVARLPIPPHQDEFDLPQ
VNAINCINAIFVEPKLSVHCTSFVSEALELALLNFDCDIWALRNCSIMLFTSLQNRIF
GKVGRSVSAKLFFTKYSGLRQLLLNILNSSIAQYSGSERKSYQIESIFLVLNVLLRLR
PTAGYTGLKEFNVSVYECLSNENWKIRDMASRVLHMLSENFEEEIRKLLDLASIAKQN
QLHGHLLALQQLVPQYLSGTRDMELIQRILEKKRMLLLENKCFITKKAYLKLTCCILE
TCDIPDSILKDYISTLRNTFIAENNEYVVDGSKQLYLAQILDMLLKYEDSIYLDDICL
LGLYSPFYEAQLSTLQYMNTNFHWETTRNSEFLEQLQLLLRVPDLLPMAKALVVKILS
RKKNTLSLTTCTDLLKTNNSEDTKLAAVSSLSAKLSSQTFHQVWNLLQGFFADSCSKD
FRLASLECLTAYPESCKNSRILLQLYNFLWDDDSEIREKASFYLNKNFIQTADWEYNR
NTSVTALIFTKKFVDVFTSSEVVEELCLQLFQYLNEYDMFAAEESAKNCLFTIEKDNQ
FINELQKAMHILNMIKLTGRDISKCYKDQIHHLKSALLEHFNTEDFKDSPLGWCSNAE
IFSRITLLKELIQHYSPSDYENFINVLTKHSVHPLIISYSQL"
assembly_gap 754436..754455
/estimated_length=20
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(754626..755087)
/gene="Tif11"
/locus_tag="P301_M13001"
mRNA complement(754626..755087)
/gene="Tif11"
/locus_tag="P301_M13001"
/product="Tif11p"
CDS complement(754626..755087)
/gene="Tif11"
/locus_tag="P301_M13001"
/note="Similar to YMR260C 'Translation initiation factor
eIF1A, essential protein that forms a complex with Sui1p
(eIF1) and the 40S ribosomal subunit and scans for the
start codon; C-terminus associates with Fun12p (eIF5B); N
terminus interacts with eIF2 and eIF3'"
/codon_start=1
/product="Tif11p"
/protein_id="EWG89517.1"
/translation="MGKKNTKGGKKGRRGKNDSDGPKRELIYKEEGQEYAQITKMLGN
GRVEASCFDGNKRMAHIRGKLRKKVWMGQGDIILVSLRDFQDDQCDVVHKYNLDEART
LKNQGELPENAKINETDNFGFESDEDVNFEFGNADEDDEEGEDEELDIDDI"
gene complement(755454..758618)
/gene="Tps3"
/locus_tag="P301_M13006"
mRNA complement(755454..758618)
/gene="Tps3"
/locus_tag="P301_M13006"
/product="Tps3p"
CDS complement(755454..758618)
/gene="Tps3"
/locus_tag="P301_M13006"
/note="Similar to YMR261C 'Regulatory subunit of
trehalose-6-phosphate synthase/phosphatase complex, which
synthesizes the storage carbohydrate trehalose; expression
is induced by stress conditions and repressed by the
Ras-cAMP pathway'"
/codon_start=1
/product="Tps3p"
/protein_id="EWG89518.1"
/translation="MTIIVASLFLPYTPQFEADVTNSDTAKLVESSMIKVDCNNQELS
NNKQERSSSVTSASSHYIGLPQEAQINGEPLQRANVGSPATGVNYHNEMEMLSSEQFL
EELTANATHAANSGIPPANNPVSSGSTAQRPSVEEFFSAPSARVCSPSQEASASSISA
SRSSAHHNDLSSSLMKNPNLSFDSHPPRVRSSSKSAVITPVSKSVPDVDPAVVDVAKV
REEFQQQASLPSMKRVSGSTAGDSSIASSSSNLRYSQQFQDNFIEDTDSEDDIDSDLE
TDATKKYNVPKFGGYSNNAKLRASLMRNSYELFKHLPWTIVDSDKGNGSLKNAVNIAV
AEKTVKEPVSWVGTMGIPTDELPHEVCHKISKKLEQDFSSFPVVTDDITFKGAYKNYA
KQILWPTLHYQIPDNPNSKAFEDHSWDYYQKVNQKFSDRIVSVYKPGDTIWIHDYHLM
LVPQMVREKLPKAKIGFFLHVSFPSSEVFRCLANRERILEGIIGANFVGFQTKEYKRH
FLQTCNRLLAADVSNDEVKYHCNIVSVMYAPIGIDYYHLTSQLRNGSVLEWRQLIKER
WRNKKLIVCRDQFDRIRGLQKKMLAYERFLIENPEYIEKVVLIQICIGKSSDPEYERQ
IMVVVDRINSLSSNISISQPVVFLHQDLDFAQYLALNCEADVFLVDALREGMNLTCHE
FIVSSFEKNAPLLLSEFTGSSSVLKEGAILINPWDINHVAQSIKRSLEMSPEEKRRRW
KKLFKSVIEHDSDNWITKCFEYINNAWESNQETSTVFNLAPEKFCADYKASKKHLFIF
KISEPPTSRMLSLLSELSSNNIVYVLSSFTKNTFESLYNGVLNIGLIAENGAYVRVNG
SWYNIVEELDWMKEVAKIFDEKVERLPGSYYKIADSMIRFHTENADDQDRVPTVIGEA
ITHINTLFDDRDIHAYVHKDIVFVQQTGLALAAAEFLMKFYNSGVSPTDNSRISLSRT
SSSMSVGNNKKHFQNQVDFVCVSGSTSPIIEPLFKLVKQEVEKNNLKFGYTILYGSSR
STYAKEHINGVNELFTILHDLTAA"
gene 758975..759916
/locus_tag="P301_M13011"
mRNA 758975..759916
/locus_tag="P301_M13011"
/product="hypothetical protein"
CDS 758975..759916
/locus_tag="P301_M13011"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89519.1"
/translation="MNKLVDAHCHVITDPDNTFCGDDGGSQGTLRCVMSSNPYDWNNL
KKLAGRSTSKNDICVGFGVHPWYSHLFYVGSRRDKVSHYQDVLEYKNEEQFDSLVQVL
PEPLDLEEYIKREFNDTLVSVIGEIGLDKLFRLPANGFYMQNEKARLTTVKVKLSHQE
TVFRRFCRLARHTSKPISIHDVKCHGKLNDICNEELLTYHSVKICLHSYTGSKETLLG
QWLKKFPPDRIFVSLSKWINFKDPEEGDALVRSLPSTCILTETDYPIDNPDPSYQKAL
TEQLQYLNAQIARAWDETLDASQAALRVYENFQKFIK"
gene 760168..760773
/gene="Sap30"
/locus_tag="P301_M13016"
mRNA 760168..760773
/gene="Sap30"
/locus_tag="P301_M13016"
/product="Sap30p"
CDS 760168..760773
/gene="Sap30"
/locus_tag="P301_M13016"
/note="Similar to YMR263W 'Subunit of a histone
deacetylase complex, along with Rpd3p and Sin3p, that is
involved in silencing at telomeres, rDNA, and silent
mating-type loci; involved in telomere maintenance'"
/codon_start=1
/product="Sap30p"
/protein_id="EWG89520.1"
/translation="MARPVNTNAETESRGRPTQGGGYASNNNGSCNNNNGSNNNNNNN
NNNNNNSNNSNNNNGPTSSGRTNGKQRLTAAQQQYIKNLIETHITDNHPDLRPKSHPM
DFEEYTDAFLRKYKDHFQLDVPDNLTLQGYLLGSKLGAKTYSYKRNTQGQHDKRIHKR
DLANVVRRHFDEHSIKETDCIPQFIYKVKNQKKKFKMEFRG"
gene 761054..761665
/gene="Cue1"
/locus_tag="P301_M13021"
mRNA 761054..761665
/gene="Cue1"
/locus_tag="P301_M13021"
/product="Cue1p"
CDS 761054..761665
/gene="Cue1"
/locus_tag="P301_M13021"
/note="Similar to YMR264W 'Endoplasmic reticulum membrane
protein that recruits the ubiquitin-conjugating enzyme
Ubc7p to the ER where it functions in protein degradation;
contains a CUE domain that binds ubiquitin to facilitate
intramolecular monoubiquitination'"
/codon_start=1
/product="Cue1p"
/protein_id="EWG89521.1"
/translation="MEDSRLLITLILVFGVIFLKKFFQSNQHPSAQRLSATGVNAHGR
PQGSTQNALRRTGRVNGGHPVTTQMVETVQNLAPNLHPEQIRYSLENTGSVEETVERY
LRGDEFSFPPGFEPSRAPMGANAAVDNNAAGGGEFNDPRKKNMICAENLLDKFHVDLN
EDMSNLSFKDLDIEERKRLLVWQARKNLETKLQSDKDLQSLLT"
gene complement(761789..763174)
/locus_tag="P301_M13026"
mRNA complement(761789..763174)
/locus_tag="P301_M13026"
/product="hypothetical protein"
CDS complement(761789..763174)
/locus_tag="P301_M13026"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89522.1"
/translation="MEEFEEFRRKGEMSSRCGNHRVLRKWNSCACELAVPFEVPEHAI
TKLHIYDFDNTLFATPGPTEQLYTRELLNLLTSSTLPNGGWWNEPGFLQAAIEISKTK
PRRYSWNADIVKLAEESYSAKDTISIVLTGREESKFHKLIEHALQTARSHWKCSENEF
RFNAVCLKKRAISEYTSKYKKELMRDFLEYYPSLRELSIYDDRIHQIDAFKSFFHSLD
LPRLKWSAIPVRPFTKSLPREQELEMVMDMVRKNNSQALSTSQKFDLRRTPRQIGYIL
CTASHRLLSIEVIKYLKRRKGRRTFRPKLYEHPLYIPCAEPGKDIPALEIAKVWSNND
TRTFDSEKKVQHISQIFYLEQPGKCIVHFQVTDLAVIASAHHXXRKPLEVYFKATPEP
NRYTFTLFPEYIVTGHFYKRDRIEDLEVVTERLINCKEDIHWVPLDNTIPIKAFFGRF
AKLAAIPCSNA"
gene 763768..>766625
/gene="Rsn1"
/locus_tag="P301_M13036"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 766626..766808
/estimated_length=183
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 767023..767955
/gene="Ppa2"
/locus_tag="P301_M13041"
mRNA 767023..767955
/gene="Ppa2"
/locus_tag="P301_M13041"
/product="Ppa2p"
CDS 767023..767955
/gene="Ppa2"
/locus_tag="P301_M13041"
/note="Similar to YMR267W Mitochondrial inorganic
pyrophosphatase, required for mitochondrial function and
possibly involved in energy generation from inorganic
pyrophosphate"
/codon_start=1
/product="Ppa2p"
/protein_id="EWG89523.1"
/translation="MNLLRMNALTSKARSIERLKQTLNILSIRNHRQFSTIQQGSKYT
LGFKKYLTLLNGEVGSFFHDVPLDLNEHEKTVNMIVEVPRWTTGKFEISKELRFNPIV
QDTKNGKLRFVNNIFPYHGYIHNYGAIPQTWEDPTIEHKLGKCDVALKGDNDPLDCCE
IGSDVLEMGSIKKVKVLGSLALIDDGELDWKVIVIDVNDPLSSKIDDLEKIEEYFPGI
LDTTREWFRKYKVPAGKPLNSFAFHEQYQNSNKTIQTIKKCHNSWKNLISGSLQEKYD
NLPNTERAGNGVTLEDSVKPPSQIPPEVQKWYYV"
gene complement(768139..769473)
/gene="Prp24"
/locus_tag="P301_M13046"
mRNA complement(768139..769473)
/gene="Prp24"
/locus_tag="P301_M13046"
/product="Prp24p"
CDS complement(768139..769473)
/gene="Prp24"
/locus_tag="P301_M13046"
/note="Similar to YMR268C Splicing factor that reanneals
U4 and U6 snRNPs during spliceosome recycling"
/codon_start=1
/product="Prp24p"
/protein_id="EWG89524.1"
/translation="MEYGHHARPDSKRPLDEGSPAAAGLTSKKANEALTRNRELTTVL
VKNLPKSYNQNKVYKYFKHCGPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHK
VVGQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRF
AYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLS
TELLDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCAFMVFENKDSAERALQMNRSL
LGNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQE
EIHINEXXXXKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDMKR
YYNNQQNHSMKHVKPSCINMMEKGPNLQVKKKIPDKQEQMSNDDFRKMFLGE"
gene 769707..770342
/gene="Tma23"
/locus_tag="P301_M13051"
mRNA 769707..770342
/gene="Tma23"
/locus_tag="P301_M13051"
/product="Tma23p"
CDS 769707..770342
/gene="Tma23"
/locus_tag="P301_M13051"
/note="Similar to YMR269W 'Nucleolar protein implicated in
ribosome biogenesis; deletion extends chronological
lifespan'"
/codon_start=1
/product="Tma23p"
/protein_id="EWG89525.1"
/translation="MDSKEYLISYGWKEGEAFREGGLKRPILVKHKRDKKGLGNAPGG
NDGEAWWERLFDGHLKNLDVSTDSNNGSIKFTQNEAVATAVSKSSSPLYRWFVKGEGL
KGTITNLGKKEEASFVVSSASSSKGKKRRRRDEDDNKVKRKKLKKDKKTSNDSESKKK
KKKKSKKESKKGKKSKHSSDEGDKSKHKKSKKSKKHKKEESSARRDRKEHI"
gene complement(770577..771673)
/gene="Rrn9"
/locus_tag="P301_M13056"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(772115..772798)
/gene="Ura10"
/locus_tag="P301_M13061"
mRNA complement(772115..772798)
/gene="Ura10"
/locus_tag="P301_M13061"
/product="Ura10p"
CDS complement(772115..772798)
/gene="Ura10"
/locus_tag="P301_M13061"
/note="Similar to YMR271C 'Minor orotate
phosphoribosyltransferase (OPRTase) isozyme that catalyzes
the fifth enzymatic step in the de novo biosynthesis of
pyrimidines, converting orotate into
orotidine-5'-phosphate; major OPRTase encoded by URA5'"
/codon_start=1
/product="Ura10p"
/protein_id="EWG89526.1"
/translation="MSASTTSLEEYQKTFLELGLECKALRFGSFKLNSGRQSPYFFNL
SLFNSGKLLANLATAYATAIIQSELKFDVIFGPAYKGIPLAAIVCVKLAEIGGTKFQG
IQYAFNRKKVKDHGEGGIIVGASLEDKRVLIIDDVMTAGTAINEAFEIISIAQGRVVG
CIVALDRQEVIHESDPERTSATQSVSKRYNVPVLSIVSLTQVVQFMGNRLSPEQKSAI
ENYRKAYGI"
gene complement(773495..773566)
/locus_tag="P301_M13066"
tRNA complement(773495..773566)
/locus_tag="P301_M13066"
/product="tRNA-Gln"
/note="TQ(CUG)M"
assembly_gap 773759..774097
/estimated_length=339
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(774873..776027)
/gene="Scs7"
/locus_tag="P301_M13076"
mRNA complement(774873..776027)
/gene="Scs7"
/locus_tag="P301_M13076"
/product="Scs7p"
CDS complement(774873..776027)
/gene="Scs7"
/locus_tag="P301_M13076"
/note="Similar to YMR272C Sphingolipid alpha-hydroxylase,
functions in the alpha-hydroxylation of
sphingolipid-associated very long chain fatty acids, has
both cytochrome b5-like and hydroxylase/desaturase
domains, not essential for growth"
/codon_start=1
/product="Scs7p"
/protein_id="EWG89527.1"
/translation="MSTNTSKTLELFSKKTVQEHNTANDCWVTYQNRKIYDVTRFLSE
HPGGDESILDYAGKDITEIMKDSDVHEHSDSAYEILEDEYLIGYLATDEEAARLLTNK
NHKVEVQLSADGTEFDSTTFVKELPAEEKLSIATDYSNDYKKHKFLDLNRPLLMQILR
SDFKKDFYVDQIHRPRHYGKGSAPLFGNFLEPLTKTAWWVVPVAWLPVVVYHMGVALK
NMNQLFACFLFCVGVFVWTLIEYGLHRFLFHFDDWLPESNIAFATHFLLHGCHHYLPM
DKYRLVMPPTLFVILCAPFYKLVFALLPLYWAYAGFAGGLFGYVCYDECHFFLHHSKL
PPFMRKLKKYHLEHHYKNYQLGFGVTSWFWDEVFGTYLGPDAPLSKMKYE"
gene 775717..775830
/locus_tag="P301_M13081"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 776339..776446
/locus_tag="P301_M13086"
mRNA 776339..776446
/locus_tag="P301_M13086"
/product="hypothetical protein"
CDS 776339..776446
/locus_tag="P301_M13086"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89528.1"
/translation="MRSLVFVQLSLLSWEIFCGERSFVSMKAIFSCMYV"
gene complement(776483..779248)
/gene="Zds1"
/locus_tag="P301_M13096"
mRNA complement(776483..779248)
/gene="Zds1"
/locus_tag="P301_M13096"
/product="Zds1p"
CDS complement(776483..779248)
/gene="Zds1"
/locus_tag="P301_M13096"
/note="Similar to YMR273C 'Protein with a role in
regulating Swe1p-dependent polarized growth; interacts
with silencing proteins at the telomere; has a role in
Bcy1p localization; implicated in mRNA nuclear export;
involved in mitotic exit through Cdc14p regulation'"
/codon_start=1
/product="Zds1p"
/protein_id="EWG89529.1"
/translation="MSNRDNESMLRTTSSDKAIASQRDKRKSEVLIAAQSLDNEIRSV
KNLKRLSIGSMDLLIDPELDIKFGGESSGRRSWSGTTSSSASMPSDTTTVNNTRYSDP
TPLENLHGRGNSGIESSNKTKQGNYLGIKKGVHSPSRKLNANVLKKNLLWVPANQHPN
VKPDNFLELVQDTLQNIQLSDNGEDNDGNSNENNDIEDNGEDKESQSYENKENNTINL
NRGLSRHGNASLIRRPSTLRRSYTEFDDNEDDDNKGDSASETVNKVEERISKIKERPV
SLRDITEELTKISNSAGLTDNDAITLARTLSMAGSYSDKKDQPQPEGHYDEGDIGFST
SQANTLDDGEFASNMPINNTMTWPERSSLRRSRFNTYRIRSQEQEKEVEQSVDEMKND
DEERLKLTKNTIKVEIDPHKSPFRQQDEDSENMSSPGSIGDFQDIYNHYRQSSGEWEQ
EMGIEKEAEEVPVKVRNDTVEQDLELREGTTDMVKPSATDDNKETKRHRRRNGWTWLN
NKMSREDDNEENQGDDENEENVDSQRMELDNSKKHYISLFNGGEKTEVSNKEEMNNSS
TSTATSQTRQKIEKTFANLFRRKPHHKHDASSSPSSSPSSSPSIPNNDAVHVRVRKSK
KLGNKSGREPVEPIVLRNRPRPHRHHHSRHGSQKISVKTLKDSQPQQQIPLQPQLEGA
IEIEKKEESDSESLPQLQPAVSVSSTKSNSRDREEEEAKKKNKKRSNTTEISNQQHSK
HVQKENTDEQKAQLQAPAQEQVQTSVPVQASAPVQNSAPVQTSAPVQTSAPVEASAQT
QAPAAPPLKHTSILPPRKLTFADVKKPDKPNSPVQFTDSAFGFPLPLLTVSTVIMFDH
RLPINVERAIYRLSHLKLSNSKRGLREQVLLSNFMYAYLNLVNHTLYMEQVAHDKEQQ
QQQQQQP"
gene complement(779631..780578)
/gene="Rce1"
/locus_tag="P301_M13101"
mRNA complement(779631..780578)
/gene="Rce1"
/locus_tag="P301_M13101"
/product="Rce1p"
CDS complement(779631..780578)
/gene="Rce1"
/locus_tag="P301_M13101"
/note="Similar to YMR274C Type II CAAX prenyl protease
involved in the proteolysis and maturation of Ras and the
a-factor mating pheromone"
/codon_start=1
/product="Rce1p"
/protein_id="EWG89530.1"
/translation="MLQFSTFLVLLYISISYVLPLYATSQPEGSKRDNPRTIKSRMQK
LTIMLISNLFLVPFLQSQLSSTTSHISFKDAFLGLGIIPGYYAALPNPWQFSQFVKDL
TKCVAMLLTLYCGPVLDFVLYHLLNPKSSILEDFYHEFLNIWSFRNFIFAPITEEIFY
TSMLLTTYLNLIPHSQLSYQQLFWQPSLFFGLAHAHHAYEQLQEGSMTTVSILLTTCF
QILYTTLFGGLTKFVFVRTGGNLWCCIILHALCNIMGFPGPSRLNLHFTVVDKKAGRI
SKLVSIWNKCYFALLVLGLISLKDTLQTLVGTPGYRITL"
gene complement(780917..783847)
/gene="Bul1"
/locus_tag="P301_M13106"
mRNA complement(780917..783847)
/gene="Bul1"
/locus_tag="P301_M13106"
/product="Bul1p"
CDS complement(780917..783847)
/gene="Bul1"
/locus_tag="P301_M13106"
/note="Similar to YMR275C Ubiquitin-binding component of
the Rsp5p E3-ubiquitin ligase complex, functional homolog
of Bul2p, disruption causes temperature-sensitive growth,
overexpression causes missorting of amino acid permeases"
/codon_start=1
/product="Bul1p"
/protein_id="EWG89531.1"
/translation="MAKDLNDSGFPPKRKPLLRPQRSDFTANSSTTMNVNANTRGRGR
QKQEGGKGSSRSPSLHSPKSWIRSASATGILGLRRPELAHSHSHAPSTGTPAGGNRSP
LRRSTANATPVETGRSLTDGDINNVVDVLPSFEMYNTLHRHIPQGNVDPDRHDFPPSY
QEANNSTATGAAGSSADLSHQSLSTDALGATRSSSTSNLENLIPLRTEHHSIAAHQST
AVDEDSLDIPPILDDLNDTDNIFIDKLYTLPKMSTPIEITIKTTKHAPIPHVKPEEES
ILKEYTSGDLIHGFITIENKSQANLKFEMFYVTLESYISIIDKVKSKRTIKRFLRMVD
LSASWSYSKIALGSGVDFIPADVDYDGSVFGLNNSRVLEPGVKYKKFFIFKLPLQLLD
VTCKQEHFSHCLLPPSFGIDKYRNNCKYSGIKVNSVLGCGHLGTKGSPILTNDMSDDN
LSINYTIDARIVGKDQKASKLYIMKEREYNLRVIPFGFDANVVGERTTMSQLNDITKL
VQERLDALRKIFQRLEKKEPITNRDIHGADLSGTIDDSIESDSQEILQRKLDQLHIKN
RNNYLVNYNDLKLGHDLDNGRSGNSGHNTDTSRAWGPFVESELKYKLKNKSNSSSFLN
FSHFLNSSSSSMSSSSNAGKNNHDLTGNKERTGLILVKAKIPKQGLPYWAPSLLRKTN
VFESKSKHDQENWVRLSELIPEDVKKPLEKLDLQLTCIESDNSLPHDPPEIQSITTEL
ICITAKSDNSIPIKLNSELLMNKEKLTSIKALYDDFHSKICEYETKFNKNFLELNELY
NMNRGDRRPKELKFTDFITSQLFNDIESICNLKVSVHNLSNIFKKQVSTLKQHSKHAL
SEDSISHTGNGSSSSPSSASLTPVTSSSKSSLFLPSGSSSTSLKFTDQIVHKWVRIAP
LQYKRDINVNLEFNKDIKETLIPSFESCLCCRFYCVRVMIKFENHLGVAKIDIPISVR
QVTK"
gene 784093..785214
/gene="Dsk2"
/locus_tag="P301_M13111"
mRNA 784093..785214
/gene="Dsk2"
/locus_tag="P301_M13111"
/product="Dsk2p"
CDS 784093..785214
/gene="Dsk2"
/locus_tag="P301_M13111"
/note="Similar to YMR276W Nuclear-enriched ubiquitin-like
polyubiquitin-binding protein, required for spindle pole
body (SPB) duplication and for transit through the G2/M
phase of the cell cycle, involved in proteolysis,
interacts with the proteasome"
/codon_start=1
/product="Dsk2p"
/protein_id="EWG89532.1"
/translation="MSLNIHIKSGQDKWEVNVAPESTVLQFKEAINKANGIPVANQRL
IYSGKILKDDQTVESYHIQDGHSVHLVKSQPKPQTASAAGANNATATGAAAGTGATPN
MSSGQSAGFNPLADLTSARYAGYLNMPSADMFGPDGGALNNDSNNQDELLRMMENPIF
QSQMNEMLSNPQMLDFMIQSNPQLQAMGPQARQMLQSPMFRQMLTNPDMIRQSMQFAR
MMDPNAGMGSAGGAASAFPAPGGDAPEEGSNTNTTSSSNTGNNAGTNAGTNAGANTAA
NPFASLLNPALNPFANAGNAASTGMPAFDPALLASMFQPPVQASQAEDTRPPEERYEH
QLRQLNDMGFFDFDRNVAALRRSGGSVQGALDSLLNGDV"
gene 785521..787719
/gene="Fcp1"
/locus_tag="P301_M13116"
mRNA 785521..787719
/gene="Fcp1"
/locus_tag="P301_M13116"
/product="Fcp1p"
CDS 785521..787719
/gene="Fcp1"
/locus_tag="P301_M13116"
/note="Similar to YMR277W Carboxy-terminal domain (CTD)
phosphatase, essential for dephosphorylation of the
repeated C-terminal domain of the RNA polymerase II large
subunit (Rpo21p)"
/codon_start=1
/product="Fcp1p"
/protein_id="EWG89533.1"
/translation="MTTQIRSPQGLPYPIQIDKLIPSVGSYLHEGDRLLVYKFWYLVE
RASDTGDDDNEHDVSPGGSAGSNGVSPPTKQLRESIEFFESPYEGDLISWNVDVGDEV
ATANQVICEIKRPCNHDIVYGGLCTQCGKEVSADAFDGVPLDVVGDVDLQISETEAIR
TGKALKEHLRRDKKLILVVDLDQTIIHCGVDPTIAEWKNDPNNPNFETLRDVKSFTLD
EELVLPLMYMNDDGSMLRPPPVRKCWYYVKVRPGLKEFFAKVAPLFEMHIYTMATRAY
ALQIAKIVDPTGELFGDRILSRDENGSLTTKSLAKLFPTDQSMVVVIDDRGDVWNWCP
NLIKVVPYNFFVGVGDINSNFLPKQSTGMLQLGRKTRQKSQESQELLTDIMDNEKKLQ
EKIDKEVKRQEEKLNHQLATAEEPPANESKEELTKKLEYSASLEVQQQNRPLAKLQKH
LHDQKLLVDDDDELYYLMGTLSNIHKTYYDMLSQQNEPEPNLMEIIPSLKQKVFQNCY
FVFSGLIPLGTDIQRSDIVIWTSTFGATSTPDIDYLTTHLITKNPSTYKARLAKKFNP
QIKIVHPDWIFECLVNWKKVDEKPYTLIVDSPISDEELQNFQTQLQKRQEYLEETQEQ
QHMLTSQENLNLFAAGTSWLNNDDDEDIPDTASDDDEDDDHDDESDDENNSEGIDRKR
SIEDNHDDTSQKKTKAEPSQDGPVQHKGEGDDNEDSDSQLEEELMDMLDD"
gene 788020..789888
/gene="Pgm3"
/locus_tag="P301_M13121"
mRNA 788020..789888
/gene="Pgm3"
/locus_tag="P301_M13121"
/product="Pgm3p"
CDS 788020..789888
/gene="Pgm3"
/locus_tag="P301_M13121"
/note="Similar to YMR278W 'Phosphoglucomutase, catalyzes
interconversion of glucose-1-phosphate and
glucose-6-phospate; transcription induced in response to
stress; green fluorescent protein (GFP)-fusion protein
localizes to the cytoplasm and nucleus; non-essential'"
/codon_start=1
/product="Pgm3p"
/protein_id="EWG89534.1"
/translation="MLQGILETVPSDLKDPISLWFKQDRNPKTIEEVTALCKKSDWNE
LHKRFDSRIQFGTAGLRSQMQAGFSRMNTLVVIQASQGLATYVRQQFPDNLVAVVGHD
HRFHSKEFARATAAAFLLKGFKVHYLNPDHEFVHTPLVPFAVDKLKASVGVMITASHN
PKMDNGYKVYYSNGCQIIPPHDHAISDSIDANLEPWANVWDFDDVLNKALKQGKLMYS
REEMLKLYLEEVSKNLVEINPLKLEVKAKPWFVYTPMHGVGFDIFSTIVKKTLCLVEG
KDYLCVPEQQNPDPSFPTVGFPNPEEKGALDIGINLAEKHDIDLLVANDPDADRFSVA
VKDMQSGEWRQLTGNEIGFLFAFYEYQKYKSMDKEFQHVHPLAMLNSTVSSQMIKKMA
EIEGFHYEDTLTGFKWIGNRAILLEKKGYYVPFGFEEAIGYMFPAMEHDKDGISASIV
FLQAYCKWKIDHNLDPLNVLENGFKKYGVFKEYNGYYVVPNPTVTKDIFDYIRNVYTP
EGASYPSSIGEEIEVLYYRDLTTGYQSDTINHKPTLPVDPTSQMITVSARPSNGSENE
HIRFTIRGSGTEPKLKVYIEACANEEQRASFLAKLTWNVLRREWFRPDEMNIVTKF"
gene complement(789986..791608)
/locus_tag="P301_M13131"
mRNA complement(789986..791608)
/locus_tag="P301_M13131"
/product="hypothetical protein"
CDS complement(789986..791608)
/locus_tag="P301_M13131"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89535.1"
/translation="MFSIFKKKTSVQGTDSEIDEKITVKAKDKVVVSTEDEEVTTIVS
STKSTQVTNDSPWQDPTYFSSFGKELMFIATCMLAQLLNQAGQTHALCIMNVLSKSFN
SEANNQAWLMASFPLAAGSFILISGRLGDIYGLKKMLIVGYVIVIVWSIISGLSKYSN
SDAFFITSRAFQGVGIAFILPNIMGLVGHVYKVGSFRKNIVISFIGACAPTGGMFGGL
FGGLIVTEDPNQWPWVFYAFGIATFLSLLMAWYSIPNNVPTNIHGLSMDWTGSALAII
GLILFNFVWNQAPIVGWDKPYIIVLLIISVIFLVAFFVYESKYAEVPLLPRAMTKNRH
MIMILLAVFLGWGSFGIWTFYYVSFQLNLRHYSPVWTGGTYFVFVIFGSMAAFFVAFS
IKRLGPALLLCFSLMAFDAGSIMFSVLPVEQSYWKLNFAMQAILCFGMDLSFPASSII
LSDGLPMQYQGMAGSLVNTVINYSASLCLGMGGTVEHQINKSGNDLLKGYRAAVYLGV
GLASLGVVISVTYMLENLWNRHRKSEDRSLEA"
gene complement(792285..796586)
/gene="Cat8"
/locus_tag="P301_M13146"
mRNA complement(792285..796586)
/gene="Cat8"
/locus_tag="P301_M13146"
/product="Cat8p"
CDS complement(792285..796586)
/gene="Cat8"
/locus_tag="P301_M13146"
/note="Similar to YMR280C Zinc cluster transcriptional
activator necessary for derepression of a variety of genes
under non-fermentative growth conditions, active after
diauxic shift, binds carbon source responsive elements"
/codon_start=1
/product="Cat8p"
/protein_id="EWG89536.1"
/translation="MANNNSDRQGLEPRVIRTLGSQALSGPSISNRTSSSEANPHFSK
NVKEAMIKTASPTPLSTPIYRIAQACDRCRSKKTRCDGKRPQCSQCAAVGFECRISDK
LLRKAYPKGYTESLEERVRELEAENKRLLALCDIKEQQISLVSQSRPQTSTDNTINGN
FKHDLKDAPLNLSSTNIYLLNQTVNKQLQNGKMDGDNSGSAMSPLGAPPPPPHKDHLC
DGVSCTNHLHVKPTSTSLNDPTAISFEQDEAPGLPAVKALKSMTAHQRSTQLATLVSL
SIPRSTEEILFIPQLLTRIRQIFGFNSKQCLYTVSLLSSLKNRLPAPRLLAPSTSTKL
KEKDEDKKLDDDSAFVKMFQSTNLSEFVDLKKFLISLKFNINSFSKQSEKPANDQDDE
LLSLTEIKELLHLFFKFWSNQVPILNNDHFLIYFNNFVEVVKHLSTENLETNNTTKST
VTTNHEIFALKLLMMLQMGLLVKIKMEKIKYTVPKNPKAKYARLMAYYHQLSLIIPKN
PYFLNMSTTSLPSLQLLSLASFYYLNVGDISAIYGVRGRIVSMAQQLRLHRCPSAVLS
VHSNPVLQKFEQSERRLLFWAIYYVDVFASLQLGVPRLLKDFDIECALPISDVEYKDQ
LSMENEKADKKAKKIQLQGQVSSFSLQIIRFAKILGNILDSIFKRGMMDERITSEVAL
VHENALDNWRNRLPEMYYFQITVNGTVNLDEIRATNQRNTETKFDKKDIILFEKKILL
LFYFLAKSMIHLPVIATKPLPKNVDNATKKKQSMFNNDSKGATNQDHMILDVDMTSPA
IRTSSSYIILQQATNATLTIFQAINSMYLPLPLNVSRTLIRFSLLFARGSLEYTKGGA
LFLDNKNLLLDTIKDIENDRLLDLPGIASWHTLKLFDMSINLLLKAPNVKVERLDKFL
EKKLNYYNRLMGLPPATTTSLKPLFGSQSKNSLENRQRTPNVKRENPEHEYLYGNDSN
NNNNSEAGHSPMTNTTNGNKRLKYEKDAKRNAKDGGISKGENAHNFQNDTKKNMSTSN
LFPFSFSNTDLTALFTHPEGPNCTNTNNGNVDVCNRASTDATDANIENLSFLNMAPFL
QTGSSNIGQNTIENKPMHMDAIFSLPSNLDLMKDNMDSKPEQLEPVIKQNPENSKNNQ
FHQKGKSTNMEKNNLSFNNKSNYSLTKLMRLLNNDNSFSNISINNFLYQNDQNSASAD
PGTNKKAVTNAGANFKSPSTGSNTSQGSILGSTKHGMDNCDFNDLGNFNNFMTNVNYS
GVDYDYIVDASLGLAPLLVDTPDISNTNTTSTTSNRSKNSIILDTTFNDDLDRSRMNA
REVLNPTDSILSQGMVSSVSTRNTSNQRSLSSGNDSKGDSSSQENSKSATGNQLDTSS
TLFQMRRTSSGPSASHRGPRRPQKNRYNTDRSKSSGGGSSNTDNVSDLFQWQNAK"
gene 797596..798510
/gene="Gpi12"
/locus_tag="P301_M13156"
mRNA 797596..798510
/gene="Gpi12"
/locus_tag="P301_M13156"
/product="Gpi12p"
CDS 797596..798510
/gene="Gpi12"
/locus_tag="P301_M13156"
/note="Similar to YMR281W 'ER membrane protein involved in
the second step of glycosylphosphatidylinositol (GPI)
anchor assembly, the de-N-acetylation of the
N-acetylglucosaminylphosphatidylinositol intermediate;
functional homolog of human PIG-Lp'"
/codon_start=1
/product="Gpi12p"
/protein_id="EWG89537.1"
/translation="MKMLRRTKVNFSKLLYKITKLAIVLTILYIYFTPKIVSRNNASL
QHIFPHKYGDYEINLVIAHPDDEVMFFSPIISQLNSYFPRTVPFNIICLSKGNAEGLG
ETRVRELNESAALLLHNERAVSVQVMDFQDGMDEIWDIDSITSSLSQKIDIKNHNLNQ
IIVTFDSYGVSNHINHKSCYAAVKKLVDDYAQPKTKRNEQPPHVTALYLRSYKNNIVL
KYNSFIWEILKILYDLISPFRRIIQALPPNTAAEKDKLSLMNTHAQYVLAFATMLNAH
ESQVVWFRYGWWIFSRFVFVNEFDVYTY"
gene complement(798613..800355)
/gene="Aep2"
/locus_tag="P301_M13161"
mRNA complement(798613..800355)
/gene="Aep2"
/locus_tag="P301_M13161"
/product="Aep2p"
CDS complement(798613..800355)
/gene="Aep2"
/locus_tag="P301_M13161"
/note="Similar to YMR282C 'Mitochondrial protein, likely
involved in translation of the mitochondrial OLI1 mRNA;
exhibits genetic interaction with the OLI1 mRNA
5'-untranslated leader'"
/codon_start=1
/product="Aep2p"
/protein_id="EWG89538.1"
/translation="MWINRLVKHPSYSVLRFYTKRLCTVSVKSLREFGVLPNSTICHS
VYPRRTYVMGRAVINDILIKKSYSTHTVCAIDRSKDENNGSAYDKFEAKGIPIDVHTL
KRIISSSGMDESEFSKSISYLFAKTVDPEPKDVLSLEDLSFLLSKLYTQRFQIRRICR
DINAKYSEFWFKLFSLYAEKVDAKRNQVNLRNTKLDACEIFDANLMIKNFIELGQLGK
AQKILSFILDRNPDILLSPKNADISTIVHFLQLRCGALAPYWKIPDNSEQKQGFLRKM
VRLGAKNTSIRLSSTYKAMDHQTLLKIADLALQEKKLLNSEDLLSTLIQSFGHLGQTQ
ILERCIEHIWQISPQEFPSHVVIKHRGCYPSSKILVSILVSFYFNDHDLHRGLSILDS
FIKHYPDVKLDALFWRRLFQLSHFAWTPANDKKATSVVRCWHLMKQWYASKRLRPSVD
YETLRQLYDIMKKTGNFPLGIDVLRSFKPGIERTRAENAGKVNNIIIKYQKCIIKELV
NRGRFSAVREFIDSYGFDRKMTKDLNIFCANRMFLRSKKMKNKIENKKEREKVRLDSF
DDDEDDGMIIGSLW"
gene complement(800583..802124)
/gene="Rit1"
/locus_tag="P301_M13166"
mRNA complement(800583..802124)
/gene="Rit1"
/locus_tag="P301_M13166"
/product="Rit1p"
CDS complement(800583..802124)
/gene="Rit1"
/locus_tag="P301_M13166"
/note="Similar to YMR283C 2'-O-ribosyl phosphate
transferase, modifies the initiator methionine tRNA at
position 64 to distinguish it from elongator methionine
tRNA"
/codon_start=1
/product="Rit1p"
/protein_id="EWG89539.1"
/translation="MVSRLSKRYRSRVQFSISVMDENIYLSLSQINKDIRKENKSVRN
RLQSILLDNKFLQDRVIPIFPHYPLIPNERCGLWYCNPSSFKQTSYFKSTDGHVNQWD
FSTRRLNFHLLETIRDNKGIIIVDSTRRGKKIPDALSKTVPIWCAVLNTLMLQETEKN
VAIDKVLYLPPETVPKSEYDMIKRKIPELVAKLQKLNIIDSKKLNELFMGKLLRPIWV
HPGSSLLDHSVDYFTGEVQEYEAWETPEDQNIIPIILCTVSYQAQDGMDKRYGFTYVQ
GAADDHELWSFGLDSNMFWAHIEYLGDASYSDDQLHDYIMDLAAAKLRNQCYIQDKGS
LDEVFGNIDKITNEISLGKVSSGLTINKNLKQKLKSEYGKVIIFSNSVTVAEDTDDEE
ESGTDPFISIYKLQSGDKKSSKALRSTFPRIHGEIQSLFTNRDEKIKPMLICCNTGTD
MSIGVILSILCTKYTEEWMLTSELPDISKLIVRKHLTKLISHLKGRNVNPSRATLNSV
NSFLM"
gene 803147..803235
/locus_tag="P301_M13176"
tRNA 803147..803235
/locus_tag="P301_M13176"
/product="tRNA-Tyr"
/note="TY(GUA)M2"
gene 803406..805215
/gene="Yku70"
/locus_tag="P301_M13181"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 805116..805147
/estimated_length=32
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(805364..806911)
/gene="Ngl2"
/locus_tag="P301_M13186"
mRNA complement(805364..806911)
/gene="Ngl2"
/locus_tag="P301_M13186"
/product="Ngl2p"
CDS complement(805364..806911)
/gene="Ngl2"
/locus_tag="P301_M13186"
/note="Similar to YMR285C 'Protein involved in 5.8S rRNA
processing; Ccr4p-like RNase required for correct 3'-end
formation of 5.8S rRNA at site E; similar to Ngl1p and
Ngl3p'"
/codon_start=1
/product="Ngl2p"
/protein_id="EWG89540.1"
/translation="MTQDKEVKVVAPDVAPDQEVEINKSVKDAKHQTNDDSLLQHKKK
GKKGKKSKPIVTPEHIANVRAEREVMRKAKRDAMLAQGVDPDCPPELHFIRRPFLSLH
EAEPVTGFRFKLMTYNCLAQALIRRKLFPDSGDALKWYRRSKVLLNEFKYYNSDVICL
QEIDHIQFQSFWKDEFSKLGYDGQYYRNATKNHGVAIMWRRELFHQVDKMLIDYDKES
SESISTRTTTNNVGLVLALKFSEKVLSNLGKKSSKKCGILIGTTHLFWHPFGTYERTR
QCYIVLKKMKEFMHRVNVLQNENDGDLSHWFPFFCGDFNSQPFDTPYLSMTSKPVHYR
NRAKTVIGCSTSYKFSKVRDGEEGADDEEGGNIEKYGKDQPESPVPEKFHANEEQSEL
VDKMAQLHNSLDMRAISLYSVGYKNVHPENAGLDNDRGEPEISNWANTWRGLLDYLFY
VKKWDPQSNCQEVETLGDFEKENKVKCRGFLRMPPGNEMTKHGQPHVGEYASDHLSMV
CDLELQL"
gene 807162..807422
/gene="Mrpl33"
/locus_tag="P301_M13191"
mRNA 807162..807422
/gene="Mrpl33"
/locus_tag="P301_M13191"
/product="Mrpl33p"
CDS 807162..807422
/gene="Mrpl33"
/locus_tag="P301_M13191"
/note="Similar to YMR286W Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl33p"
/protein_id="EWG89541.1"
/translation="MVFYKVTLSRSLIGVPHTTKSIVKSLGLGKRGSIVYKKVNPAIA
GSLAKVKELVKVEVTEHELTPSQQRELRKSNPGFIVEKRTID"
gene complement(807656..810565)
/gene="Dss1"
/locus_tag="P301_M13196"
mRNA complement(807656..810565)
/gene="Dss1"
/locus_tag="P301_M13196"
/product="Dss1p"
CDS complement(807656..810565)
/gene="Dss1"
/locus_tag="P301_M13196"
/note="Similar to YMR287C '3'-5' exoribonuclease,
component of the mitochondrial degradosome along with the
ATP-dependent RNA helicase Suv3p; the degradosome
associates with the ribosome and mediates turnover of
aberrant or unprocessed RNAs'"
/codon_start=1
/product="Dss1p"
/protein_id="EWG89542.1"
/translation="MVVRRKVHVLLIARSFHSYTPCFRVTTRGKRQRSKSKQQAKVEL
DHTRELDNDQATETVVDRSVGPEKDIESINKDFLQRTKGLEPDIELKQLPQIKQEFNQ
RYKDRYVKPSEDWYVNSWRSLTKPKIPLYKLINSDFQLITKLKAPNPMEFQPVQLMES
PLNVGDFVLLKMRPNELAMCVSLPSSTMDPRYTFVTIDGTMCFATKNRVLLRIPHKLP
AGIRSLIQPESHHKHLPIGTVKNFSNQTNILPIVARQLITGRYPAQISKLAWKDLPIT
TKKLQLLHRSLQNYMGPWQIPFFTLVGLVQKLDLNKALDDKNGINYLTSLVNNYHTVN
DIPINSPTFVSTYWAIMQQQESNLWGEIHLNTALLSPISVTIIPLKSQHLYYAQVIEK
LEANSYREVNKFVKLVNERKYRDISALYPSVIQLLKDFAAGNFHNNGIIVALISKIFR
KIERYKDCDITRDICQDLINEITPNSIPNPLLLNMDLALPASSKLVKWQQKLYDLTNI
EELQWKKSGTDDDRYDFGDLRVFCIDSETAHEIDDGVSVKNYGRDGLYTLYIHIADPT
SMFPESTNVDIEGISTDILNVALKRSFTTYLPDTVVPMLPQSICHLSDLGKQGQRTKT
ISFSVDVKITSKCSGKSIEIMYDSFKIRKGIVSNFPKATYEDVDRILSSPNGEASPVK
KDLEGLSMISKLLREQRIKNSNAVIFGEGFNKGLVMLNADSEGELTEVTFSDQEETLS
TILVSEMMILANTLTGRYFAENKIGGVFRCYKQLPLGEVAQQQYDSMITSTKKGIFPK
LKDIVKLSSLLNSSFYTGRPFRHEMIGAKQYLTVTSPLRRFPDLINHLQIHRHLQKKP
LCFNQTQIDSLIWPIQSRADILKRASRNSSTYWTLNYLKKLTKLEPERTFDVMVTSVP
QNGFTGCVFPDLSFARGTLKLHPSSMHYPMIGDIVKNCKISKIDCLEGMLELEKL"
gene 810791..813706
/gene="Hsh155"
/locus_tag="P301_M13201"
mRNA 810791..813706
/gene="Hsh155"
/locus_tag="P301_M13201"
/product="Hsh155p"
CDS 810791..813706
/gene="Hsh155"
/locus_tag="P301_M13201"
/note="Similar to YMR288W 'U2-snRNP associated splicing
factor that forms extensive associations with the branch
site-3' splice site-3' exon region upon prespliceosome
formation; similarity to the mammalian U2 snRNP-associated
splicing factor SAP155'"
/codon_start=1
/product="Hsh155p"
/protein_id="EWG89543.1"
/translation="MSHPIQFVNANSSDKSHQLGGQYSISQDLRENLQKEAAHIGENE
KDVLQEKMETRTVQNREDSYHKRRFDMKFEPDSDTQTVTSSENTQDAVVPRKRKSRWD
VKGYEPPDESSTAVKENSDSALVNVEGIHDLMFFKPSDHKYFADIISKKPIDELNKDE
KKERTLSMLLLKIKNGNTASRRTSMRILTDKAVTFGPEMIFNRLLPILLDRSLEDQER
HLMIKTIDRVLYKLGDLTKPYVHKILVVAAPLLIDEDPMVRSTGQEIITNLSTVAGLK
TILTVMRPDIENEDEYVRNVTSRAAAVVAKALGVNQLLPFINAACHSRKSWKARHTGI
KIVQQIGILLGIGVLNHLTGLMSCIKDCLMDDHVPVRIVTAHTLSTLAENSYPYGIEV
FNVVLEPLWKGIRSHRGKVLSSFLKAVGSMIPLMDPEYAGYYTTEAMRIIRREFDSPD
DEMKKTILLVLQKCSAVESITPKFLREEIAPEFFQKFWVRRVALDRPLNKVVTYTTVT
LAKKLGCSYTIDKLLTPLRDEAEPFRTMAVHAVTRTVNLLGTADLDERLETRLIDALL
IAFQEQTNSDSIIFKGFGAVTVSLDIRMKPFLAPIVSTILNHLKHKTPLVRQHAADLC
AILIPVIKNCHEFEMLNKLNIILYESLGEVYPEVLGSIINAMYCITSVMDLDKLQPPI
NQILPTLTPILRNKHRKVEVNTIKFVGLIGKLAPTYAPPKEWMRICFELLELLKSTNK
EIRRSANATFGFIAEAIGPHDVLVALLNNLKVQERQLRVCTAVAIGIVAKVCGPYNVL
PVIMNEYTTPETNVQNGVLKAMSFMFEYIGNMSKDYIYFITPLLEDALTDRDLVHRQT
ASNVITHLALNCSGTGHEDAFIHLINLLIPNIFETSPHAIMRILEGLEALSQALGPGL
FMNYIWAGLFHPAKNVRKAFWRVYNNMYVMYQDAMVPFYPVTPDNNEEYIEELDLVL"
gene 813907..815031
/gene="Abz2"
/locus_tag="P301_M13206"
mRNA 813907..815031
/gene="Abz2"
/locus_tag="P301_M13206"
/product="Abz2p"
CDS 813907..815031
/gene="Abz2"
/locus_tag="P301_M13206"
/note="Similar to YMR289W 'Aminodeoxychorismate lyase
(4-amino-4-deoxychorismate lyase), catalyzes the third
step in para-aminobenzoic acid biosynthesis; involved in
folic acid biosynthesis'"
/codon_start=1
/product="Abz2p"
/protein_id="EWG89544.1"
/translation="MSLMDNWKTDMESYDEGGLVANPNFEVLATFRYDPGFARQSASK
KEIFETPDPRLGLRDEDIRQQIINEDYSSYLRVREVNSGGDLLENIQHPDAWKHDCKT
IVCQRVEDMLQVIYERFFLLVEQYQRIRIALSYFKIDFSTSLNDLLKLLVENLINCKE
GNAEYHEKIQKMFNERQCYKMRVLVSKTGNIRIEAIPMPMEPILKLTTDYDSVSTYFI
KTMLNGFLIDSTINWDVVVSSEPLNASAFTSFKTTSRDHYARARVRMQTAINNLRGSE
PTSSVSQCEILFSNKSGLLMEGSITNVAVIQKDPNGSKKYVTPRLATGCLCGTMRHYL
LRLGLIEEGDIDIGSLTVGNEVLLFNGVMGCIKGTVKTKY"
gene complement(815297..816814)
/gene="Has1"
/locus_tag="P301_M13211"
mRNA complement(815297..816814)
/gene="Has1"
/locus_tag="P301_M13211"
/product="Has1p"
CDS complement(815297..816814)
/gene="Has1"
/locus_tag="P301_M13211"
/note="Similar to YMR290C 'ATP-dependent RNA helicase;
localizes to both the nuclear periphery and nucleolus;
highly enriched in nuclear pore complex fractions;
constituent of 66S pre-ribosomal particles'"
/codon_start=1
/product="Has1p"
/protein_id="EWG89545.1"
/translation="MATPLNKRSRDSESTEEPVVDEKSTSKQNNAAPEGEQTTCVEKF
EELKLSQPTLKAIEKMGFTTMTSVQARTIPPLLAGRDVLGAAKTGSGKTLAFLIPAIE
LLHSLKFKPRNGTGIIVITPTRELALQIFGVARELMEFHSQTFGIVIGGANRRQEAEK
LMKGVNMLIATPGRLLDHLQNTKGFVFKNLKALIIDEADRILEIGFEDEMRQIIKILP
NEDRQSMLFSATQTTKVEDLARISLRPGPLFINVVPETDNSTADGLEQGYVVCDSDKR
FLLLFSFLKRNQKKKIIVFLSSCNSVKYYAELLNYIDLPVLELHGKQKQQKRTNTFFE
FCNAERGILICTDVAARGLDIPAVDWIIQFDPPDDPRDYIHRVGRTARGTKGKGKSLM
FLTPNELGFLRYLKASKVPLNEYEFPENKIANVQSQLEKLIKSNYYLHQTAKDGYRSY
LQAYASHSLKTVYQIDKLDLAKVAKSYGFPVPPKVNITIGASGKTPNTKRRKTHK"
gene 816646..816991
/locus_tag="P301_M13216"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 817852..819612
/locus_tag="P301_M13221"
mRNA 817852..819612
/locus_tag="P301_M13221"
/product="hypothetical protein"
CDS 817852..819612
/locus_tag="P301_M13221"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89546.1"
/translation="MTTASSSASQLQQRLPEEKPWPQLSGSNADAQTFKCKYVTNHNS
LGDGNFSVVKECMNIHTKDLYAMKLIKKQTVKNKIQLIQREFDLLRSISEKIRDMEKK
NEHSLDIFEGHHHILQLFDYFETADNIVLITQLCQKGDLYEKIVENQCLDLETQVTSY
CACLVSVLEFLHSQGIVHRDLKAENVLFRLRVNENEKNLQGEHHGDFKYDLLAHDLVL
ADFGLAAEYNTSKVNSLKEFVGTISYIAPEIVKCKGVGEMTPDQVGKLDKYGCPVDIW
ALGVLTYFMAFGYTPFDCTTDDETLECISKCDYYVDEQMMHDPKYEQFWNFVQCCFTI
DPAVRRSAKNLKQHPFIKDYFATSNSLNTKDTPNFSFHPTIRRVSSTASMHTLRSPSK
SRKTTTLAYLNMDGGSSETSTAFSSKMDLPDLYVDRTINSRERSLNRIRDTLKKTLSM
TSLKPAGTFDYLHANKNGTSLSSSKSGLVKKNSTFVLDPKPPKNSLMNGCFSTTPESR
SNFNTPKTLSRQGSSTSVKKYVNEVDLLLTPRTASMSSNDTTAINDYDTTNDKNPARK
HAASFQVNVDDSDGDETMQI"
gene 820013..820510
/gene="Got1"
/locus_tag="P301_M13226"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(820616..822009)
/gene="Lrc6"
/locus_tag="P301_M13231"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 822180..823301
/gene="Jnm1"
/locus_tag="P301_M13236"
mRNA 822180..823301
/gene="Jnm1"
/locus_tag="P301_M13236"
/product="Jnm1p"
CDS 822180..823301
/gene="Jnm1"
/locus_tag="P301_M13236"
/note="Similar to YMR294W 'Component of the yeast dynactin
complex, consisting of Nip100p, Jnm1p, and Arp1p; required
for proper nuclear migration and spindle partitioning
during mitotic anaphase B'"
/codon_start=1
/product="Jnm1p"
/protein_id="EWG89547.1"
/translation="MNVIDLSDPAINVDYDSLIGIDNEESQEIFENEVKEDGQQEEQE
EASSRKDGLIVEPGRDVESLRRAIRDQLLFKIHRQNQSDCADARKLSNDEEDESRQQK
LERIREELEELKIENLTSEMQTEIKELCEIQSKLATESSSRLTNLRKKLLETYEGQDT
VILPNIILDTSNIKRLQKLDQKISLMERFVGIPEALEAEEDRKSVHSKVNELYRSIQL
LQGDDKAEGKLQKFRDRLVELNEEFENSLLGKKIQQDLRLKDDTVSKLVMPENKVKEI
NSMYSMFKQYQDSLPLLAERMKSLNKMNNRVIEVYETTKGLDSQITSIQEQGKVWLKA
LNELDKKFDEQEVKIRENMEQIRRKIDTLEDEALQRNSK"
gene 823425..823781
/locus_tag="P301_M13241"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 823465..823494
/estimated_length=30
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(823512..824102)
/locus_tag="P301_M13246"
mRNA complement(823512..824102)
/locus_tag="P301_M13246"
/product="hypothetical protein"
CDS complement(823512..824102)
/locus_tag="P301_M13246"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89548.1"
/translation="MMHFRKKSSISNTSDHDGANRASDVKISEDDKARLKMRTASVAD
PILDAVQEAQPFEQAADTFHDNMNRQSYFSNEEGHVLCDVFGQPITQADISNPTRARD
ERPLDTIRSFEYAVSGDPVWAQQLETPTYGFRVRPDFPVFGAAVTYDANGMPQQVGGA
SSQMYGEQAVYQPQQHVQTEEKQKKKKGLFGRMKKK"
gene complement(824425..826101)
/gene="Lcb1"
/locus_tag="P301_M13251"
mRNA complement(824425..826101)
/gene="Lcb1"
/locus_tag="P301_M13251"
/product="Lcb1p"
CDS complement(824425..826101)
/gene="Lcb1"
/locus_tag="P301_M13251"
/note="Similar to YMR296C Component of serine
palmitoyltransferase, responsible along with Lcb2p for the
first committed step in sphingolipid synthesis, which is
the condensation of serine with palmitoyl-CoA to form
3-ketosphinganine"
/codon_start=1
/product="Lcb1p"
/protein_id="EWG89549.1"
/translation="MAHIPEVLPKSIPIPAFIVTTSSYLWYYFNLVLTQIPGGQFIVS
YIKKSHHDDPYRTTVEIGLILYGIIYYLSKPQQKKSLQAQKPNLSPQEIDALIEDWEP
EPLVDPSATDEQSWRVAKTPVTMEMPIQNHITITRNNLQEKYTNVFNLASNNFLQLSA
REPVKEVVKTTIKNYGVGACGPAGFYGNQDVHYTLEYDLAQFFGTQGSVLYGQDFCAA
PSVLPAFTKRGDVIVADDQVSLPVQNALQLSRSTVYYFNHNDMNSLECLLNELTEQEK
LEKLPAIPRKFIVTEGIFHNSGDLAPLPELTKLKNKYKFRLFVDETFSIGVLGATGRG
LSEHFNMDRATAIDITVGSMATALGSTGGFVLGDSVMCLHQRIGSNAYCFSACLPAYT
VTSVSKVLKLMDSNNDAVQTLQKLSKSLHDSFASDDSLRSYVIVTSSPVSAVLHLQLT
PAYRSRKFGYTCEQLFETMSALQKKSQTNKFIEPYEEEEKFLQSIVDHALINYNVLIT
RNTIVLKQETLPIVPSLKICCNAAMSPEELKNACESVKQSILACCQESNK"
gene 827130..828728
/gene="Prc1"
/locus_tag="P301_M13256"
mRNA 827130..828728
/gene="Prc1"
/locus_tag="P301_M13256"
/product="Prc1p"
CDS 827130..828728
/gene="Prc1"
/locus_tag="P301_M13256"
/note="Similar to YMR297W 'Vacuolar carboxypeptidase Y
(proteinase C; CPY), broad-specificity C-terminal
exopeptidase involved in non-specific protein degradation
in the vacuole; member of the serine carboxypeptidase
family'"
/codon_start=1
/product="Prc1p"
/protein_id="EWG89550.1"
/translation="MKAFTSLLCGLGLSTTLAKAISLQRPLGLDKDVLLQAAEKFGLN
LDLDHLLKELDSNVLDAWAQIEHLYPNQVMSLETSTKPKFPEAIKTKKDWDFVVKNDA
IENYQLRVNKIKDPKILGIDPNVTQYTGYLDVEDEDKHFFFWTFESRNDPAKDPVILW
LNGGPGCSSLTGLFFELGPSSIGPDLKPIGNPYSWNSNATVIFLDQPVNVGFSYSGSS
GVSNTVAAGKDVYNFLELFFDQFPEYVNKGQDFHIAGESYAGHYIPVFASEILSHKDR
NFNLTSVLIGNGLTDPLTQYNYYEPMACGEGGEPSVLPSEECSAMEDSLERCLGLIES
CYDSQSVWSCVPATIYCNNAQLAPYQRTGRNVYDIRKDCEGGNLCYPTLQDIDDYLNQ
DYVKEAVGAEVDHYESCNFDINRNFLFAGDWMKPYHTAVTDLLNQDLPILVYAGDKDF
ICNWLGNKAWTDVLPWKYDEEFASQKVRNWTASITDEVAGEVKSYKHFTYLRVFNGGH
MVPFDVPENALSMVNEWIHGGFSL"
gene 829026..829478
/gene="Lip1"
/locus_tag="P301_M13266"
mRNA 829026..829478
/gene="Lip1"
/locus_tag="P301_M13266"
/product="Lip1p"
CDS 829026..829478
/gene="Lip1"
/locus_tag="P301_M13266"
/note="Similar to YMR298W 'Ceramide synthase subunit;
single-span ER membrane protein associated with Lag1p and
Lac1p and required for ceramide synthase activity, null
mutant grows extremely slowly and is defective in ceramide
synthesis'"
/codon_start=1
/product="Lip1p"
/protein_id="EWG89551.1"
/translation="MSQPTPIITTKSAAKPKPKIFNLFRVCFISLLLIAAVEYFKYGT
RINYEWFHCTPIKELQSGSVIKLWARGGPSCDKRGEYKTIVKRITRDYEPNDEHLSFC
IIENDNVPPVHYPIHEDKGEPGYVAYVGYDTDSELVQELCADSTIYHM"
gene complement(829619..830557)
/gene="Dyn3"
/locus_tag="P301_M13271"
mRNA complement(829619..830557)
/gene="Dyn3"
/locus_tag="P301_M13271"
/product="Dyn3p"
CDS complement(829619..830557)
/gene="Dyn3"
/locus_tag="P301_M13271"
/note="Similar to YMR299C 'Dynein light intermediate chain
(LIC); localizes with dynein, null mutant is defective in
nuclear migration'"
/codon_start=1
/product="Dyn3p"
/protein_id="EWG89552.1"
/translation="MDNCNAWDKLLSQNESTINSTETATITAIIYSPSSKTLHQFINI
CFPEGSNSILDTTLINYATIGWTNDLKENYSVDVYTLIRNTDDALDLLKPFLQEHSSK
VRWLILLDWTLNDQKLWLNELSYAFNKIKQLNDDNEFSVWCLNSGEILNLQRHTTVWQ
SVHIDFILQTLRSFCYFNDSSLFYICEDHTEEKREEAQRLKYQELLKHFCEDRDMKDH
IEMVKRSEILIPKGCDSIGLIKTVDERFEPTEVKEQHFLARYMDFIPTIDKIREDRKT
TSGIDLDKLYPLEVFKVNIQEELGKMFAKYRENSRI"
assembly_gap 830566..830650
/estimated_length=85
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(830769..832301)
/gene="Ade4"
/locus_tag="P301_M13281"
mRNA complement(830769..832301)
/gene="Ade4"
/locus_tag="P301_M13281"
/product="Ade4p"
CDS complement(830769..832301)
/gene="Ade4"
/locus_tag="P301_M13281"
/note="Similar to YMR300C 'Phosphoribosylpyrophosphate
amidotransferase (PRPPAT; amidophosphoribosyltransferase),
catalyzes first step of the 'de novo' purine nucleotide
biosynthetic pathway'"
/codon_start=1
/product="Ade4p"
/protein_id="EWG89553.1"
/translation="MCGILGIVLANQTTPVAPELCDGCIFLQHRGQDAAGIATCGSRG
RIYQCKGNGMARDVFTQQRVSGLAGSMGIAHLRYPTAGSSANSEAQPFYVNSPYGINL
AHNGNLVNTASLKRYMDEDVHRHINTDSDSELLLNIFAAELEKHNKYRVNNEDVFHAL
EGVYRLCRGGYACVGLLAGFALFGFRDPNGIRPLLFGERENPDGTKDYMLASESVVFK
AHNFTKYRDLKPGEAVIIPKNCSKGEPEFKQVVPINSYRPDLFEYVYFARPDSVLDGI
SVYHTRLAMGSKLAENILKQLKPEDIDVVIPVPDTARTCALECANVLGKPYREGFVKN
RYVGRTFIMPNQRERVSSVRRKLNPMESEFKGKKVLIVDDSIVRGTTSKEIVNMAKES
GATKVYFASAAPAIRYNHIYGIDLTDTKNLIAYNRTDEEVAEVIGCERVIYQSLEDLI
DCCKTDKITKFEDGVFTGNYVTGVEDGYIQELEEKRESIANNSSDMKAEVDIGLYNCA
DY"
gene complement(832762..834833)
/gene="Atm1"
/locus_tag="P301_M13286"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(835279..837831)
/gene="Yme2"
/locus_tag="P301_M13291"
mRNA complement(835279..837831)
/gene="Yme2"
/locus_tag="P301_M13291"
/product="Yme2p"
CDS complement(835279..837831)
/gene="Yme2"
/locus_tag="P301_M13291"
/note="Similar to YMR302C 'Integral inner mitochondrial
membrane protein with a role in maintaining mitochondrial
nucleoid structure and number; mutants exhibit an
increased rate of mitochondrial DNA escape; shows some
sequence similarity to exonucleases'"
/codon_start=1
/product="Yme2p"
/protein_id="EWG89554.1"
/translation="MLLVRTTSLNVSRMPVPCLARGIGILKGKYRLANLMNAQPSVRH
VSSEIQQKDQQAGESNTATDTGVIHKSDEETLIYFDNVYARATSVWNPTLWYNLLLRN
QSRDAVREKIRNLASPPNNPIYGLELKSTIPVKRDGGVFATFVVPPKYTKAQVNSLIQ
QNTARESSKNLLSYFTRASAFPVKGSPWIEDLRRLPSTTIVIKFQGPALTEEEIYSLF
RRYGTIIDIFPPTAANNNVAKVRYRSFRGAISAKNCVSGIEIHNTVLHIQYENIIRGH
LVSNFFTNHTRIAIPVLFALLSIFAVLVFDPIREFSIEQKITHKYSLSWDNKFWKQLK
TLTSSTMTSIKYYWGGPDDNHQRKHLWEERIEKVNDLKMWLEENNNTFVVIRGPRGSG
KHDLVMQHTLQNRANVLYLDCDKLIKSRTDPKFLKNAASQLGYFPIFPWIDSVTGVLD
LTVQGLTGQKTGLSETKESQFRNMLTTSLMSIRRIALKNYKAFVSTGDGTVNVKEEDY
LQQHPEAKPVIVIDRFEGKSEINGFVYKELSDWAAMLVQMNIAHVIFLTETVASNQRL
SESLPNQVFKNLILSDASKENSRNYVLSQLEDYLYYNKKSKGENVKEPESEKETAENN
DSDSEADTSVKKAEVILNEKELQEIDASLEPLGGRMLDLQAFVRRVKSGEEPSEALDK
MIEQASEQITQMFLSDKIDSNKSAQAWELIELLSANPVIPFHEIVNKPLFKAAPETGI
MELENNGLITVSRDRGVLQEIRPAKPLYRAAFTYLINDPELAKVLKTRYLLKVVGFET
GRIKKWEEELKPLGKVPDQKLFKTRLDYLSGKINASNAVITKCEEEIKNLSK"
gene complement(838497..839543)
/gene="Adh2"
/locus_tag="P301_M13306"
mRNA complement(838497..839543)
/gene="Adh2"
/locus_tag="P301_M13306"
/product="Adh2p"
CDS complement(838497..839543)
/gene="Adh2"
/locus_tag="P301_M13306"
/note="Similar to YMR303C 'Glucose-repressible alcohol
dehydrogenase II, catalyzes the conversion of ethanol to
acetaldehyde; involved in the production of certain
carboxylate esters; regulated by ADR1'"
/codon_start=1
/product="Adh2p"
/protein_id="EWG89555.1"
/translation="MSIPETQKAIIFYESNGKLEYKDIPVPKPKANELLINVKYSGVC
HTDLHAWHGDWPLPVKLPLVGGHEGAGVVVGMGENVKGWKIGDYAGIKWLNGSCMACE
YCELGKESNCPHADLSGYTHDGSFQEYATADAVQAAHIPQGTDLAEVAPVLCAGITVY
KALKSANLRAGHWVAISGAAGGLGSLAVQYAKAMGYRVLGIDGGPGKEELFTSLGGEV
FIDFTKEKDIVSAVVKATNGGAHGIINVSVSEAAIEASTRYCRANGTVVLVGLPAGAK
CSSDVFNHVVKSISIVGSYVGNRADTREALDFFARGLVKSPIKVVGLSSLPEIYEKME
KGQIAGRYVVDTSK"
gene 840189..843881
/gene="Ubp15"
/locus_tag="P301_M13311"
mRNA 840189..843881
/gene="Ubp15"
/locus_tag="P301_M13311"
/product="Ubp15p"
CDS 840189..843881
/gene="Ubp15"
/locus_tag="P301_M13311"
/note="Similar to YMR304W Ubiquitin-specific protease that
may play a role in ubiquitin precursor processing"
/codon_start=1
/product="Ubp15p"
/protein_id="EWG89556.1"
/translation="MSSEDELGSIGTVFPGSPIDKSIGSILPQFDEEVETLLEDSFTW
NIPDWNELTNPKYNSPRFRIGDFEWDILLFPQGNHNKGVAVYLEPHPEEKLDETTGEM
VPVDPDWYCCAQFAIGISRPGNGDTINLINKSHHRFNALDTDWGFANLIDLNNLKHPS
KGRPLSFLNEGTLNITAYVRILKDPTGVLWHNFLNYDSKKVTGYVGFRNQGATCYLNS
LLQSYFFTKYFRKLVYEIPTEHESPNNSVPLALQRAFYQLQVSDIPLDTLELTRSFGW
DTAESFTQHDVQELNRILMDRLENNMKGTPVEGKLNEIFVGKMKSYIKCINVDYESAR
VEDFWDLQLNVKNFKNLQESFDNYIEMELMNGENQYAAQNYGLQDAQKGVIFESFPPV
LHLQLKRFEYDFNYDQMVKVNDKYEFPETIDLSPFVDKDVLKKTLDSENKDKNPYVYN
LHGVLVHSGDISTGHYYTLIKPGVEDQWYRFDDERVWRVTKKQVFQENFGCDRLPDEK
VRTMTRDEYQNYIIQRHTSAYMLVYIRQEQEEDLLRPVLESDVPKHVITRVREEIKER
ETKEKEIREAHLYVTLRLHSIKEFIHYEGFDYFAHDGFRLFAEELNDSGLQQINLKVL
RTTKLSDIFASIKETMNIPQERDVKYWKMDYRRNSTLRLTQPINFESVNITLQEALKK
EKKMTMQTQYGEEGVASTEEDDKALLETVSFLDLFIEEPYLELQFLNKLKEASLISKA
QLDDELISTIRTNLPELTKGGIEPVFATDNKSNLLFVKSYDPHTQKLLGFGHFAVNQL
QQLSDISAIIEDSISSNEKLTFYEEVQPGTINEIYMKETIYDADIDTGDIVSFEVPGA
VLPDTFPVYATIKDFYSYLRYRVKLKFSKFDGSSEEYGVSNEIPESFEFWISAYAPYD
DLARMVSKYAHVKPEYLKIFALYSNGRFVLKSTSLLNDYLLKDFNCDQIPPFAFEVLS
VPLKELERLRPIKLYWLKNSYIHYQCFEFEVANDYTESQFLEKVQHKIGFTDEEKENI
LLWTNTNFQFQGLLSDQNTFKDVSKHSLLFGRILPEESKLFKELNRLENVQTSSLEDF
MDDENATDRPMDDEQDLGMAIEHSEDMKGRIVVVQQYFKDLENRHGISFLFNLIPDET
FPKTKDRLHAKFGLGQKEFSKIKLSIGYSTEEGTVFRSLQGFSDEELDKVILYDIMSN
LDYIYMDHPDRLRSHSSYDRPMIIKN"
gene complement(844001..844324)
/locus_tag="P301_M13316"
mRNA complement(844001..844324)
/locus_tag="P301_M13316"
/product="hypothetical protein"
CDS complement(844001..844324)
/locus_tag="P301_M13316"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89557.1"
/translation="MIYGKMMGNTVLKNTGVFYQVIKPLKLHLLYDHSFSLFLKKQPN
MGGISPFPYLLSYSYHVIVYLCVLCKVYILYSFVKDFSICLPFSYGSSLLYSLCSNLY
ICGIT"
gene complement(844257..845426)
/gene="Scw10"
/locus_tag="P301_M13321"
mRNA complement(844257..845426)
/gene="Scw10"
/locus_tag="P301_M13321"
/product="Scw10p"
CDS complement(844257..845426)
/gene="Scw10"
/locus_tag="P301_M13321"
/note="Similar to YMR305C 'Cell wall protein with
similarity to glucanases; may play a role in conjugation
during mating based on mutant phenotype and its regulation
by Ste12p'"
/codon_start=1
/product="Scw10p"
/protein_id="EWG89558.1"
/translation="MRFSNFLTVSALLTGALGAPAVRHKHEKRDVVTATVHAQVTVVV
SGNSGETIVPVNENAVVATTSSTAVASQATTSTLEPTTSANVVTSQQQTSTLQSSEAA
STVGSSTSSSPSSSSSTSSSASSSASSSISASGAKGITYSPYNDDGSCKSTAQVASDL
EQLTGFDNIRLYGVDCSQVENVLQAKTSSQKLFLGIYYVDKIQDAVDTIKSAVESYGS
WDDITTVSVGNELVNGGSATTTQVGEYVSTAKSALTSAGYTGSVVSVDTFIAVINNPD
LCNYSDYMAVNAHAYFDENTAAQDAGPWVLEQIERVYTACGGKKDVVITETGWPSKGD
TYGEAVPSKANQEAAISSIKSSCGSSAYLFTAFNDLWKDDGQYGVEKYWGILSSD"
gene 846353..851710
/gene="Fks3"
/locus_tag="P301_M13331"
mRNA 846353..851710
/gene="Fks3"
/locus_tag="P301_M13331"
/product="Fks3p"
CDS 846353..851710
/gene="Fks3"
/locus_tag="P301_M13331"
/note="Similar to YMR306W 'Protein involved in spore wall
assembly, has similarity to 1,3-beta-D-glucan synthase
catalytic subunits Fks1p and Gsc2p; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies'"
/codon_start=1
/product="Fks3p"
/protein_id="EWG89559.1"
/translation="MDFMSPKFSLTDVEYPAWCQDDEVPITMQEIREIFVDLMDKYGF
QKSSMENMYQHLMGQLDSRASRTGAQNALVSLHVSYIGGEHANYRKWYFAAQLDLDEE
IGFQNMRLHGKARQRNVKMAKKRGVSIKEQIKQWNEKEQEFINNHPKITLTQEQLEDQ
TNLKSADYKWKLKMKKLTPENMIRQLALYLLCWGEANQVRFAPECLCFIFKCALDYDI
STSSSEKTVKSPEYSYLNDVITPLYEFLRGQVYKKDAKGNWKRREKDHKNIIGYDDIN
QLFWYPEGFERIILNNGERLVDKPLEERYLYFKDVAWSKVFYKTYRETRSWKHCFTNF
NRFWIIHFAPFWFFTTFNSPTLYTKNYIQLLNNQPTPQVRLSVIAFGGTIACLVQILA
TVFEWGFVPREWPGAQHLSSRMIGLLFCLAINLGPSVYVLGFFEWDVHSKSAYIVSIV
QLIIAFLTTFFFAVRPLGGLFRPYLNKDKKHRRYISSQTFTASFPKLTGRSKWFSYGL
WVFVYLAKYIESYFFLTLSLRDPIRVLSIMDLSRCQGEYLLGPILCKWQAKITLVLML
LSDLGLFFLDTYLWYIICNCIFSIVLSFSLGTSILTPWKNVYSRLPKRIYSKILATSE
MDVKFKAKILISQVWNAIVISMYREHLLSIEHLQRLLFQQVDSLMGDTRTLKSPTFFV
AQDDSTFKSMEFFPSNSEAKRRISFFAQSLATPISEPVPVDCMPTFTVLVPHYSEKIL
LGLKEIIREESPKSKITVLEYLKHLHPTEWECFVKDTKLLSMEKSFLKEAESSHDEDR
LEIPDALYDPRSSPLSDHTESRKLPTEDDLIKEKINDLPFSYFGFNSSEPSYTLRTRI
WASLRTQTLYRTLSGFMNYSKAIKLLYRIENPSLVSLYRGNNEALENDLENMASRKFR
MVVAMQRYAKFNKDEVEATELLLRAYPNMFISYLLEELEQNESEKTYYSCLTNGYAEF
DEESGLRKPIFKIRLSGNPILGDGKSDNQNHSIIFYRGEYIQVIDANQDNYLEECLKI
RSVLSEFEELELNPTIPYIPGIEYEEEPPPIAIVGSREYIFSENIGVLGDIAAGKEQT
FGTLFARTLAEIGGKLHYGHPDFLNGIFMTTRGGLSKAQRGLHLNEDIYAGMNAICRG
GKIKHSDYYQCGKGRDLGFGSILNFTTKIGAGMGEQLLSREYYYLGTQLPMDRFLSFF
YAHPGFHLNNLFISFSVQLFFVLLLNLGALNHEIIACFYDKDAPITNLETPVGCYNIQ
PALHWVSIFVLSIFIVFFIAFAPLLIQEVLEKGIWRAASRFLHHLLSMAPLFEVFVCQ
VYSNSLLMDLTFGGAKYISTGRGFAITRLDFFTLYSRFVNISIYSGFQVFFMLLFAII
SMWQPALLWFWITVISMCFAPFIFNPHQFAFMDFFIDYKTFIHWLFSGNTKYQKESWA
NFVKSSRSRFTGYKSKTVDDISEDSGHDSKKARFWNVFFAELFLPFCVFLFNFTAFSF
INAQTGVSDSTPTSAVFRLLLVTFLPIFLNSIVLFLLFWVSLFVVPGLSYCCKDAGAV
IAFIAHTFSVLIYLLDFELMWFLQGWNFTRTLILLITCINMHLILFKVFTTIFLTREY
KNNKAHLAWWNGKWYNTGMGWSIILQPIREYFVKIMESSYFAADFFLGHFLLFIQTPI
ILLPFIDYWHTMVLFWMNPRSIIAHKRILTRKQRALRSRIVSKYFSLYFVMLGVLLFM
LIAPFFAGDFVSSPQELLEGTLFEGIFQPNNQNNNDTGPNAPSTILTTTPTLPTFRTV
A"
gene complement(851377..851763)
/locus_tag="P301_M13341"
mRNA complement(851377..851763)
/locus_tag="P301_M13341"
/product="hypothetical protein"
CDS complement(851377..851763)
/locus_tag="P301_M13341"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89560.1"
/translation="MIGTSSLYQLLKITFFYPYATVLKVGKVGVVVRIVDGAFGPVSL
LFWLLGWKIPSKRVPSSNSCGLDTKSPAKNGAMSIKRSTPSITKYREKYFETILLLNA
LCFRVKILLCAIILLGFIQNSTIVCQ"
gene 852195..853874
/gene="Gas1"
/locus_tag="P301_M13346"
mRNA 852195..853874
/gene="Gas1"
/locus_tag="P301_M13346"
/product="Gas1p"
CDS 852195..853874
/gene="Gas1"
/locus_tag="P301_M13346"
/note="Similar to YMR307W 'Beta-1,3-glucanosyltransferase,
required for cell wall assembly and also has a role in
transcriptional silencing; localizes to the cell surface
via a glycosylphosphatidylinositol (GPI) anchor; also
found at the nuclear periphery'"
/codon_start=1
/product="Gas1p"
/protein_id="EWG89561.1"
/translation="MLFKSLSKLATAAAFFAGVATADDVPAIEVVGNKFFYSNNGSQF
YIRGVAYQADTANETSGSTVNDPLANYESCSRDIPYLKKLNTNVIRVYAINTTLDHSE
CMKALNDADIYVIADLAAPATSINRDDPTWTVDLFNSYKTVVDTFANYTNVLGFFAGN
EVTNNYTNTDASAFVKAAIRDVRQYISDKNYRKIPVGYSSNDDEDTRVKMADYFACGD
DDVKADFYGINMYEWCGKSDFKTSGYADRTAEFKNLSIPVFFSEYGCNEVTPRLFTEV
EALYGSNMTDVWSGGIVYMYFEETNKYGLVSIDGNDVKTLDDFNNYSSEINKISPTSA
NTKSYSATTSDVACPATGKYWSAATELPPTPNGGLCSCMNAANSCVVSDDVDSDDYET
LFNWICNEVDCSGISANGTAGKYGAYSFCTPKEQLSFVMNLYYEKSGGSKSDCSFSGS
ATLQTATTQASCSSALKEIGSMGTNSASGSVDLGSGTESSTASSNASGSSSKSNSGSS
GSSSSSSSSSASSSSSSKKNAATNVKANLAQVVFTSIISLSIAAGVGFALV"
gene complement(854144..857413)
/gene="Pse1"
/locus_tag="P301_M13356"
mRNA complement(854144..857413)
/gene="Pse1"
/locus_tag="P301_M13356"
/product="Pse1p"
CDS complement(854144..857413)
/gene="Pse1"
/locus_tag="P301_M13356"
/note="Similar to YMR308C 'Karyopherin/importin that
interacts with the nuclear pore complex; acts as the
nuclear import receptor for specific proteins, including
Pdr1p, Yap1p, Ste12p, and Aft1p'"
/codon_start=1
/product="Pse1p"
/protein_id="EWG89562.1"
/translation="MSALPEEVNRTLLQIVQAFASPDNQIRSVAEKALSEEWITENNI
EYLLTFLAEQAAFSQDTTVAALSAVLFRKLALKAPPSSKLMIMSKNITHIRKEVLAQI
RSSLLKGFLSERADSIRHKLSDAIAECVQDDLPAWPELLQALIESLKSGNPNFRESSF
RILTTVPYLITAVDINSILPIFQSGFTDASDNVKIAAVTAFVGYFKQLPKSEWSKLGI
LLPSLLNSLPRFLDDGKDDALASVFESLIELVELAPKLFKDMFDQIIQFTDMVIKNKD
LEPPARTTALELLTVFSENAPQMCKSNQNYGQTLVMVTLIMMTEVSIDDDDAAEWIES
DDTDDEEEVTYDHARQALDRVALKLGGEYLAAPLFQYLQQMITSTEWRERFAAMMALS
SAAEGCADVLIGEIPKILDMVIPLINDPHPRVQYGCCNVLGQISTDFSPFIQRTAHDR
ILPALISKLTSECTSRVQTHAAAALVNFSEFASKDILEPYLDSLLTNLLVLLQSNKLY
VQEQALTTIAFIAEAAKNKFIKYYDTLMPLLLNVLKVNNKDNSVLKGKCMECATLIGF
AVGKEKFHEHSQELISILVALQNSDIDEDDALRSYLEQSWSRICRILGDDFVPLLPIV
IPPLLITAKATQDVGLIEEEEAANFQQYPDWDVVQVQGKHIAIHTSVLDDKVSAMELL
QSYATLLRGQFAVYVKEVMEEIALPSLDFYLHDGVRAAGATLIPILLSCLLAATGTQN
EELVLLWHKASSKLIGGLMSEPMPEITQVYHNSLVNGIKVMGDNCLSEDQLAAFTKGV
SANLTDTYERMQDRHGDGDEYNENIDEEEDFTDEDLLDEINKSIAAVLKTTNGHYLKN
LENIWSMINTFLLDNEPILVIFALVVIGDLIQYGGEQTASMKNAFIPKVTECLISPDA
RIRQAASYIIGVCAQYAPSTYADVCIPTLDTLVQIVDFPGSKLEENRSSTENASAAIA
KILYAYNSNIPNVDTYTANWFKTLPTITDKEAASFNYQFLSQLIENNSPIVCAQSNIS
AVVDSVIQALNERSLTEREGQTVISSIKKLLGFLPSSDAMAIFNRYPADIMEKVHKWF
A"
gene complement(858177..860615)
/gene="Nip1"
/locus_tag="P301_M13361"
mRNA complement(858177..860615)
/gene="Nip1"
/locus_tag="P301_M13361"
/product="Nip1p"
CDS complement(858177..860615)
/gene="Nip1"
/locus_tag="P301_M13361"
/note="Similar to YMR309C eIF3c subunit of the eukaryotic
translation initiation factor 3 (eIF3), involved in the
assembly of preinitiation complex and start codon
selection"
/codon_start=1
/product="Nip1p"
/protein_id="EWG89563.1"
/translation="MSRFFSSNYEYDVASSSSEEDLLSSSEEDLLSSSSSESELDQES
DDSFFNESESESEADVDSDDSDAKPYGPDWFKKSEFRKQGGGSNKFLKSSNYDSSDEE
SDEEDGKKVVKSAKEKLLDEMQDVYNKISQAENSDDWLTISNEFDLISRLLVRAQQQN
WGTPNIFIKVVAQVEDAVNNTQQADLKNKAVARAYNTTKQRVKKVSRENEDSMAKFRN
DPESFDKEPTADLDISANGFTISSSQGNDQAVQEDFFTRLQTIIDSRGKKTVNQQSLI
STLEELLTVAEKPYEFIMAYLTLIPSRFDASANLSYQPIDQWKSSFNDISKLLSILDQ
TIDTYQVNEFADPIDFIEDEPKEDSDGVKRILGSIFSFVERLDDEFMKSLLNIDPHSS
DYLIRLRDEQSIYNLILRTQLYFEATLKDEHDLERALTRPFVKRLDHIYYKSENLIKI
METAAWNIIPAQYKSKFTSKDQLDSADYVDNLIDGLSTILSKQNNIAVQKRAILYNIY
YTALNKDFQTAKDMLLTSQVQTNINQFDSSLQILFNRVVVQLGLSAFKLCLIEECHQI
LNDLLSSSHLREILGQQSLHRISLNSSNNASADERARQCLPYHQHINLDLIDVVFLTC
SLLIEIPRMTAFYSGIKVKRIPYSPKSIRRSLEHYDKLSFQGPPETLRDYVLFAAKSM
QKGNWRDSVKYLREIKSWALLPNMETVLNSLTERVQVESLKTYFFSFKRFYSSFSVAK
LAELFDLPENKVVEVLQSVIAELEIPAKLNDEKTIFVVEKGDEITKLEEAMVKLNKEY
KIAKERLNPPSNRR"
gene complement(860905..861858)
/locus_tag="P301_M13366"
mRNA complement(860905..861858)
/locus_tag="P301_M13366"
/product="hypothetical protein"
CDS complement(860905..861858)
/locus_tag="P301_M13366"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89564.1"
/translation="MSSTRKFKKVEKPLSQTRHYSLCIPTTLVSDCRNLSQITHKVYQ
VAKFASLFNVSEVVILEDNSQVDATKKKISTAKLILALLQYFVTPPYLRNTVFNEKFR
PYLTAASKLPRLSTLPFTRYQKQDHGRYREGLTIKMQKPTLARKKTGKVFKQTKYINI
GKSKALALQSQLVPINARVTIDTITRKIVSPQEAYGDFTGLDSQYGYYTRIASSFTDL
FMKGPLKEGYTQSVYVPLTTRDTSIPELSSLPTAETNPHILLVFSTWDTLARAFKLDQ
DQFVDCQGPQEFFDAQLPCPVSNSDVADAIPMTLTTLSTVF"
gene complement(862103..862792)
/gene="Glc8"
/locus_tag="P301_M13371"
mRNA complement(862103..862792)
/gene="Glc8"
/locus_tag="P301_M13371"
/product="Glc8p"
CDS complement(862103..862792)
/gene="Glc8"
/locus_tag="P301_M13371"
/note="Similar to YMR311C 'Regulatory subunit of protein
phosphatase 1 (Glc7p), involved in glycogen metabolism and
chromosome segregation; proposed to regulate Glc7p
activity via conformational alteration; ortholog of the
mammalian protein phosphatase inhibitor 2'"
/codon_start=1
/product="Glc8p"
/protein_id="EWG89565.1"
/translation="MGGILKNPLALSPEQLAQQDPETLEEFRRQVYENTQKNAKLTSH
KRNIPGLDNTKEEGEIIGTSSTFLPKDTLSLKHEQDMLAKMTPEERVQWNQRNLAENE
ITKKQFQDIHIDEPKTPYQGAVDPHGEYYRVDDDEDEDNSDKKPCQVANDDIDDLSLG
EPEFEIKENKQPDFETNDENDEDSPEARHKKFEEMRKKHYDVRAIFNKKSREALKDED
EDEDDSTTKEP"
gene 863593..864414
/gene="Elp6"
/locus_tag="P301_M13381"
mRNA 863593..864414
/gene="Elp6"
/locus_tag="P301_M13381"
/product="Elp6p"
CDS 863593..864414
/gene="Elp6"
/locus_tag="P301_M13381"
/note="Similar to YMR312W 'Subunit of Elongator complex,
which is required for modification of wobble nucleosides
in tRNA; required for Elongator structural integrity'"
/codon_start=1
/product="Elp6p"
/protein_id="EWG89566.1"
/translation="MSSVQRQDLVLFSDQSVLPAHFFQDSNSHNLFFITHQSCTQPLW
MINALVETHVLGSPSSLNESSSSMLPSSTRSHAVLASFIHEQNYFTNSLNKLKIPSNN
YNVLDFLSDFIVNNIHNKPRDKILSDVLAKFSAAIQNNPTDTIVIIEQPELLLSLVSG
LTCSELNNKFITPLLRQCKVLIIVSNSDIFNIDEYDASVHSSNLQNFYKSSFIKSMIN
LNLNPLKTGFAKDVTGSLHVCRGGAPIATSNTSLHVVENEYLYLNEKESTKLFYR"
gene complement(864566..866494)
/gene="Tgl3"
/locus_tag="P301_M13391"
mRNA complement(864566..866494)
/gene="Tgl3"
/locus_tag="P301_M13391"
/product="Tgl3p"
CDS complement(864566..866494)
/gene="Tgl3"
/locus_tag="P301_M13391"
/note="Similar to YMR313C 'Bifunctional enzyme with
triacylglycerol lipase and lysophosphatidylethanolamine
acyltransferase activity; responsible for all the
triacylglycerol lipase activity of the lipid particle;
required with Tgl4p for timely bud formation'"
/codon_start=1
/product="Tgl3p"
/protein_id="EWG89567.1"
/translation="MKETAQEYKVSAVIPTLLKNWILRVVYATLDHIPPFVWEILHVI
TDIYFFWVQKLINYVRPHSRVIYYNAIKKLDECDTYQMWCQQASVVDEITGANLWRRN
FFSRRYDFNSVIEQYSILENMLREEKYDVVKEKFSTTGPCMLRNFAGIGDKKLFTKSL
MGTKLLIEQYLTRILEGLDILNNQTLTPTSFFQRCKLSLGTTALILQGGSLFGLFHLG
VIRGLLLQDLMPNIISGSSMGACVASLFGCLSNEQLKQLLTDDNLLNIIKNDVDLLKS
CGYGNLEQHLNLGTLIQNLIHHGYSQDVYLFIRFVMKYIVKEKTFEEVYQITGKVFNI
VIHPTDKSCPNLLNYVTTPNVLIKSAIECSLGSGVISEDTSLLCKNLENEIEPFLNIN
KNKQVKFLTPENANNPSITESPYTRLTELFNVNNFIVSLARPYLAPLVVNDLKHEIKT
SKYYYYKHYPNMPPINANTVRKTQRSSSQSPIKAGTVEDLEPEPLMSPAPPSSAVNDS
AEYIIPELGIPQLNFTEMEPLAFKFKYHLERKLKNIATMEFRHRMEVLDNLGLLCSLI
KRLIIDEKTPRSATEIAVVPRMKSLSLTRIIEGQLNNIPYWIKSGERSTWPALALIKT
RCAVEFKLDDIIRARRSR"
gene 866894..867598
/gene="Pre5"
/locus_tag="P301_M13396"
mRNA 866894..867598
/gene="Pre5"
/locus_tag="P301_M13396"
/product="Pre5p"
CDS 866894..867598
/gene="Pre5"
/locus_tag="P301_M13396"
/note="Similar to YMR314W Alpha 6 subunit of the 20S
proteasome"
/codon_start=1
/product="Pre5p"
/protein_id="EWG89568.1"
/translation="MFRNNYDGDTVTFSPTGRLFQVEYALEAIKQGSVTVGLRSNTHA
VLVALKRNADELSSYQKKIIKCDEHMGLSLAGLAPDARVLSNYLRQQCNYSSLVFNRK
LAVERAGHLLCDKAQKNTQSYGGRPYGVGLLIIGYDKSGAHLLEFQPSGNVTELYGTA
IGARSQGAKTYLERTLDTFIKIDGNPDELIKAGVEAISQSLRDESLTVDNLSIAIVGK
DTPFTIYDGEAVAKYI"
gene 867985..869034
/locus_tag="P301_M13406"
mRNA 867985..869034
/locus_tag="P301_M13406"
/product="hypothetical protein"
CDS 867985..869034
/locus_tag="P301_M13406"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89569.1"
/translation="MSPLNVGIVGTGIFARDRHLPSYQEFPDKFKVIAAFNRHKAKAL
DFAKVADIPENKVYDNLDEILNDPHVDYIDALLPAQFNADIVEKAVKAGKPVILEKPI
AANLDQAKEIVKIAESTPLPVGVAENWLYLPCIKIAKEQIEKIGPVVAFTHNSTGPFV
TQNKYLTTTWRQKPEHIGGFLSDGGVHQLALVISLLGEFGSVSALTRQVRERSGADDI
VFATVQLKNKEVIGSFTYGSAFGATEKSVFLKVYGKNGTVTVDLSDKKDPVVKVKLGG
SAEDNGDEQIFKVDNDESFGVNAEFLNFHEAVSKKDKSLYLGTPRTAFHHLACVDAFL
KSSAKNGDHVKIEQP"
gene 869470..869577
/locus_tag="P301_M13411"
mRNA 869470..869577
/locus_tag="P301_M13411"
/product="hypothetical protein"
CDS 869470..869577
/locus_tag="P301_M13411"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89570.1"
/translation="MTERKLLQLLRRPFISLSLFTALRACPLCPKSLIA"
gene 870008..871018
/gene="Dia1"
/locus_tag="P301_M13416"
mRNA 870008..871018
/gene="Dia1"
/locus_tag="P301_M13416"
/product="Dia1p"
CDS 870008..871018
/gene="Dia1"
/locus_tag="P301_M13416"
/note="Similar to YMR316W 'Protein of unknown function,
involved in invasive and pseudohyphal growth; green
fluorescent protein (GFP)-fusion protein localizes to the
cytoplasm in a punctate pattern'"
/codon_start=1
/product="Dia1p"
/protein_id="EWG89571.1"
/translation="MGSISRYLLKKAADGLKDEQRLKIEMSDSKSVPECFHFNRERRM
PIAEINGEDGFFMFPSQQSLENFENTKKYSNELSPDAIGIPLFQIINCTLPFGKRGHS
NTVVGNVPYYKIFKFILRTADEPPPYTVAKIVCSNNGLILYKVPLYDIYKNVSQANVT
YSFVGTTSTEPNLLAMAHREGHRDLDTKVNNLNLRWHVTYSPVVTNDHYKLILLADYE
VNRLDEDVIRAAKNKMSTDQKDQKVQRFVAAHYTREFETSLFRWVAQEGHLILGEYST
DQGSFGLNNIPPLTEELGCQSLLIHYIEYMKRQRKKIAKGARRQNKRNVANTTNMNMN
LM"
gene complement(870845..871156)
/locus_tag="P301_M13421"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(872504..872812)
/locus_tag="P301_M13431"
mRNA complement(872504..872812)
/locus_tag="P301_M13431"
/product="hypothetical protein"
CDS complement(872504..872812)
/locus_tag="P301_M13431"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89572.1"
/translation="METNDSDDRIEAIVSEESDELEVNSGTTEETNVLELLTIISGLV
VTLVLVVLVVLVVVGVGVLVVLLVVVVLLCDVVVAVVDFEPDEPILIRRYPFLWIPFL
"
gene 872547..875789
/locus_tag="P301_M13436"
mRNA 872547..875789
/locus_tag="P301_M13436"
/product="hypothetical protein"
CDS 872547..875789
/locus_tag="P301_M13436"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG89573.1"
/translation="MGSSGSKSTTATTTSHSSTTTTSSTTSTPTPTTTSTTSTTSTKV
TTSPEIIVSSSSTLVSSVVPEFTSSSSLSSDTIASILSSESLVSIFSSLSYTSSDISS
TSVNDVESSTPGPSNSYSAPSSTNAQLSSSTTETDSISSSAIQTSSPQTSSSNEGGSS
SEPLGKSSVLETTASSSDTTAVTSSTFTTLTDVSSSPKVSSSGSAVTSVGSTSDASKE
VFSSSTSDVSSLLSSTSSPASSTISETLPLSSTILSITSSSVSSEAPSATSSVMSSEV
SSATSSSVSSGISSTTSSSVSSAVPLATSSVVSSEAPSSKSSVVSSEAPSSTSSSVSS
EISSTTSSVMSSEVSSATSSSVSSEISSTTSSSVSSEVPSATSSLVSSEAPSAISSLA
SSRLFSSKNTSVTSTLVATEASSVTSSLRPSSETLASNSIIESSLSTGYNSTVSTTTS
AASSSLESKVSSSNSRMATSKTSSTSSDLSKSSVIFGNSSTVTTSPSASISLTASPLP
SVWSDITSSEASSISSNLASSSAPSDNNSTIASASLIVTKTKNSVVSSIVSSITSSET
TNESNLATSSTSLLSNKATARSLSTSNATSASNVPTGTFSSMSSHTSVITPGFSTSST
PLAINSTVVSSSLAGYSFSTPETSPTTSTLVTSEAPSTVSSMTTSAPFINNSTSARPS
PSTASFITESTSSISSVPLASGDVTSSLAAHNLTTFSAPSTSSAQLVSKSTTSSSILV
TPSIDRSANSSTASTIATSLPNKTTFVSSLSSTSAHARSIFNSTVLATAKQIKTLTST
VNCSNATPNYNITKTVIVSRETTAIGTVTSCSGGCTKNRKSTTLITVTDIDASTVTTC
PEKEVTSTTSGDEAEHTTSTKISNFETSTFSESFKDMKTSQETKKAKPGSKTVRSSSS
FVEKTSPTTKASPSTSPSESEATGNTSVATNVSPSTSPSESQGTGSTSVEGAKSKSTK
NSEGVSTTKAKNTSTVAKSSTESPIGRGETTLETIIVSGQKSLLTSQLSSSTEKVNRS
TTKPTAAIHGTSSSAKQSTTYTVSTAKENTGASLNINMKAFVIGAIALVA"
gene complement(876063..877145)
/gene="Adh6"
/locus_tag="P301_M13441"
mRNA complement(876063..877145)
/gene="Adh6"
/locus_tag="P301_M13441"
/product="Adh6p"
CDS complement(876063..877145)
/gene="Adh6"
/locus_tag="P301_M13441"
/note="Similar to YMR318C 'NADPH-dependent medium chain
alcohol dehydrogenase with broad substrate specificity;
member of the cinnamyl family of alcohol dehydrogenases;
may be involved in fusel alcohol synthesis or in aldehyde
tolerance'"
/codon_start=1
/product="Adh6p"
/protein_id="EWG89574.1"
/translation="MSYPEKFEGIAIQSHEDWKNPKKTKYDPKPFYDHDIDIKIEACG
VCGSDIHCAAGHWGNMKMPLVVGHEIVGKVVKLGPKSNSGLKVGQRVGVGAQVFSCLE
CDRCKNDNEPYCTKFVTTYSQPYEDGYVSQGGYANYVRVHEHFVVPIPENIPSHLAAP
LLCGGLTVYSPLVRNGCGPGKKVGIVGLGGIGSMGTLISKAMGAETYVISRSSRKRED
AMKMGADHYIATLEEGDWGEKYFDTFDLIVVCASSLTDIDFNIMPKAMKVGGRIVSIS
IPEQHEMLSLKPYGLKAVSISYSALGSIKELNQLLKLVSEKDIKIWVETLPVGEAGVH
EAFERMEKGDVRYRFTLVGYDKEFSD"
gene complement(877882..879540)
/gene="Fet4"
/locus_tag="P301_M13456"
mRNA complement(877882..879540)
/gene="Fet4"
/locus_tag="P301_M13456"
/product="Fet4p"
CDS complement(877882..879540)
/gene="Fet4"
/locus_tag="P301_M13456"
/note="Similar to YMR319C Low-affinity Fe(II) transporter
of the plasma membrane"
/codon_start=1
/product="Fet4p"
/protein_id="EWG89575.1"
/translation="MGKIAEFLGNPGARPDVHHRAPTVDCKQYEEFGDSNDYKNDDVV
RVVSHSDESTDDELCNVNLTETGAIFTSKGFTGLSKGFTDKTLDFLVRVAGSQAVFFI
VWIILIIWVVIGIVYNAPFNWQVVMQDGQSIQSYVWDTLLMRQQLMSTHEQILICGRL
KSRLASFKNYLTRSTPEEEKADCTVEANEVSSVENHIDPSAINGELPVENWYDRLSNV
ASRYMGSIAAMVIFWIGIFVWIGCGAIPKDAGNTPPYTGETTGSNPRLKKFSDAWQMY
INTAVAVSLLICTTFLQNIRARHDYFTGRFLVDIFDMDEKIDYRIRKHFNDFETPHPV
VTIESKKRSTGRKMIDWYADIIGTGIGVLIGVAVFATWIGIGSPMKWDDNWWLIIGTY
TGLIGFLDGFVLREVYFRIVQHEEKNYSDVAKEDLELFQELGIECPEEFSGKAPEINI
IGYRTSQYINRICSTPWSVLVSVIIIIGLICIASGLRWSTTGQLIANTPTMIIEEFFL
LVLLQAHNWADRQRRVEVTALYARRRILLSYVEKRFPEVMMLEK"
CONTIG join(APIQ01000644.1:1..12233,gap(61),APIQ01000645.1:1..18072,
gap(1054),APIQ01000646.1:1..3528,gap(227),APIQ01000647.1:1..5761,
gap(223),APIQ01000648.1:1..16322,gap(46),APIQ01000649.1:1..15180,
gap(217),APIQ01000650.1:1..5001,gap(36),APIQ01000651.1:1..8817,
gap(172),APIQ01000652.1:1..1876,gap(27),APIQ01000653.1:1..32682,
gap(35),APIQ01000654.1:1..34961,gap(32),APIQ01000655.1:1..8258,
gap(227),APIQ01000656.1:1..5855,gap(29),APIQ01000657.1:1..4025,
gap(32),APIQ01000658.1:1..1831,gap(235),APIQ01000659.1:1..25027,
gap(587),APIQ01000660.1:1..3001,gap(366),APIQ01000661.1:1..7844,
gap(199),APIQ01000662.1:1..4180,gap(413),APIQ01000663.1:1..2367,
gap(139),APIQ01000664.1:1..14265,gap(115),APIQ01000665.1:1..8516,
gap(97),APIQ01000666.1:1..2974,gap(426),APIQ01000667.1:1..6797,
gap(25),APIQ01000668.1:1..684,gap(51),APIQ01000669.1:1..21426,
gap(197),APIQ01000670.1:1..6741,gap(187),APIQ01000671.1:1..24582,
gap(11),APIQ01000672.1:1..8523,gap(23),APIQ01000673.1:1..2018,
gap(203),APIQ01000674.1:1..8838,gap(183),APIQ01000675.1:1..3530,
gap(102),APIQ01000676.1:1..912,gap(413),APIQ01000677.1:1..3528,
gap(71),APIQ01000678.1:1..7402,gap(328),APIQ01000679.1:1..14026,
gap(150),APIQ01000680.1:1..13098,gap(252),APIQ01000681.1:1..8737,
gap(67),APIQ01000682.1:1..2563,gap(76),APIQ01000683.1:1..47225,
gap(297),APIQ01000684.1:1..3332,gap(136),APIQ01000685.1:1..1537,
gap(78),APIQ01000686.1:1..7298,gap(153),APIQ01000687.1:1..4764,
gap(256),APIQ01000688.1:1..2043,gap(241),APIQ01000689.1:1..20603,
gap(1335),APIQ01000690.1:1..10038,gap(80),APIQ01000691.1:1..2466,
gap(68),APIQ01000692.1:1..2038,gap(255),APIQ01000693.1:1..746,
gap(37),APIQ01000694.1:1..2606,gap(13),APIQ01000695.1:1..718,
gap(60),APIQ01000696.1:1..11359,gap(837),APIQ01000697.1:1..2138,
gap(32),APIQ01000698.1:1..9202,gap(21),APIQ01000699.1:1..3517,
gap(379),APIQ01000700.1:1..2209,gap(20),APIQ01000701.1:1..15582,
gap(16),APIQ01000702.1:1..3576,gap(50),APIQ01000703.1:1..33843,
gap(588),APIQ01000704.1:1..2094,gap(11),APIQ01000705.1:1..6406,
gap(55),APIQ01000706.1:1..5291,gap(110),APIQ01000707.1:1..7034,
gap(46),APIQ01000708.1:1..24744,gap(90),APIQ01000709.1:1..14210,
gap(38),APIQ01000710.1:1..12474,gap(78),APIQ01000711.1:1..42474,
gap(33),APIQ01000712.1:1..8153,gap(103),APIQ01000713.1:1..4778,
gap(215),APIQ01000714.1:1..9723,gap(106),APIQ01000715.1:1..1169,
gap(354),APIQ01000716.1:1..14230,gap(514),APIQ01000717.1:1..3987,
gap(436),APIQ01000718.1:1..6509,gap(12),APIQ01000719.1:1..3346,
gap(188),APIQ01000720.1:1..3157,gap(61),APIQ01000721.1:1..7099,
gap(20),APIQ01000722.1:1..12170,gap(183),APIQ01000723.1:1..6950,
gap(339),APIQ01000724.1:1..31018,gap(32),APIQ01000725.1:1..18317,
gap(30),APIQ01000726.1:1..7071,gap(85),APIQ01000727.1:1..51835)
//