LOCUS KK037059 1041851 bp DNA linear CON 11-FEB-2014
DEFINITION Saccharomyces cerevisiae P301 chromosome VII genomic scaffold
scaffold08, whole genome shotgun sequence.
ACCESSION KK037059 APIQ01000000
VERSION KK037059.1
DBLINK BioProject: PRJNA162717
BioSample: SAMN01915094
KEYWORDS WGS.
SOURCE Saccharomyces cerevisiae P301
ORGANISM Saccharomyces cerevisiae P301
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 1041851)
AUTHORS Treu,L., Toniolo,C., Nadai,C., Sardu,A., Giacomini,A., Corich,V.
and Campanaro,S.
TITLE The impact of genomic variability on gene expression in
environmental Saccharomyces cerevisiae strains
JOURNAL Environ. Microbiol. (2013) In press
PUBMED 24238297
REMARK Publication Status: Available-Online prior to print
REFERENCE 2 (bases 1 to 1041851)
AUTHORS Campanaro,S., Treu,L. and Corich,V.
TITLE Direct Submission
JOURNAL Submitted (31-JAN-2013) Department of Biology, University of Padua,
Via U. Bassi 58/b, Padova, PD 35121, Italia
COMMENT ##Genome-Assembly-Data-START##
Assembly Method :: Newbler v. 2.3
Assembly Name :: p301
Genome Coverage :: 14.0x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..1041851
/organism="Saccharomyces cerevisiae P301"
/mol_type="genomic DNA"
/submitter_seqid="scaffold08"
/strain="P301"
/isolation_source="grape bunch from Prosecco vineyard"
/db_xref="taxon:1182968"
/chromosome="VII"
/country="Italy: Col De Roer (TV)"
/collection_date="Sep-2004"
gene 1732..2875
/gene="Cos12"
/locus_tag="P301_G10001"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 4255..4767
/locus_tag="P301_G10006"
mRNA 4255..4767
/locus_tag="P301_G10006"
/product="hypothetical protein"
CDS 4255..4767
/locus_tag="P301_G10006"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90783.1"
/translation="MRNNVTELVNSIIGVQTPGSLPDTLSGAHSLQRRISYFDVNWIS
WNWDNVNVDLNKEVKKSRPLLGEEDDQCMFGWFANNPGWKYYWSVTDNPDPGYKENYS
DIGDENAVHGELYFNTYGGLMASVMTTKMVLNAKRQLVVIDTIVVTAICDYVMKYWKK
RLTLLRYHYI"
gene complement(5226..5588)
/gene="Pau11"
/locus_tag="P301_G10011"
mRNA complement(5226..5588)
/gene="Pau11"
/locus_tag="P301_G10011"
/product="Pau11p"
CDS complement(5226..5588)
/gene="Pau11"
/locus_tag="P301_G10011"
/note="Similar to YGL261C 'Putative protein of unknown
function and member of the seripauperin multigene family
encoded mainly in subtelomeric regions; mRNA expression
appears to be regulated by SUT1 and UPC2'"
/codon_start=1
/product="Pau11p"
/protein_id="EWG90784.1"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTIAN"
gene 5796..6026
/locus_tag="P301_G10016"
mRNA 5796..6026
/locus_tag="P301_G10016"
/product="hypothetical protein"
CDS 5796..6026
/locus_tag="P301_G10016"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90785.1"
/translation="MEMLLFLNESYIFHRLRMWSIVLWHSCVFVCAECGNANYRVPRC
LIKPFSVPVTFPFSVKKNIRILDLDPRTEAYC"
gene 7406..7903
/gene="Yps5"
/locus_tag="P301_G10021"
mRNA 7406..7903
/gene="Yps5"
/locus_tag="P301_G10021"
/product="Yps5p"
CDS 7406..7903
/gene="Yps5"
/locus_tag="P301_G10021"
/note="Similar to YGL259W Protein with similarity to
GPI-anchored aspartic proteases such as Yap1p and Yap3p"
/codon_start=1
/product="Yps5p"
/protein_id="EWG90786.1"
/translation="MQLFSILSLLSSLMCSLTVLGSSASSYVKFPVQKFADIINIGTQ
DVSTVFKRNEVLNTTVINGIGVYVVKMEIGTPPQTVYLQLDTGSSDMIVNNADIAYCK
SMSDGSDYASTDNYELTATFTGPRSTTTSPELITLSALIGVNSMQETHLLLRITRLSS
MTYTY"
gene 8099..8331
/locus_tag="P301_G10026"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 9493..9511
/estimated_length=19
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 10049..10669
/gene="Vel1"
/locus_tag="P301_G10036"
mRNA 10049..10669
/gene="Vel1"
/locus_tag="P301_G10036"
/product="Vel1p"
CDS 10049..10669
/gene="Vel1"
/locus_tag="P301_G10036"
/note="Similar to YGL258W 'Protein of unknown function;
highly induced in zinc-depleted conditions and has
increased expression in NAP1 deletion mutants'"
/codon_start=1
/product="Vel1p"
/protein_id="EWG90787.1"
/translation="MSFLSIFTFFSVLVSVATAVRFDLTNVTCKGLHGPHCGTYVMEV
VGQNGTFLGQSTFVGADVLTESAGDAWARYLGQETRFLPKLTTVASNETKNFSPLIFT
TNINTCNPQSIGDAMVPFANTVTGEIEYNSWADTADNASFITGLANQLFNSTDYGVQV
ASCYPSFASVILSTPTVNIFGKDDTLPDYCTAIQLKAVCPPEAGFD"
gene complement(11428..13104)
/gene="Mnt2"
/locus_tag="P301_G10051"
mRNA complement(11428..13104)
/gene="Mnt2"
/locus_tag="P301_G10051"
/product="Mnt2p"
CDS complement(11428..13104)
/gene="Mnt2"
/locus_tag="P301_G10051"
/note="Similar to YGL257C Mannosyltransferase involved in
adding the 4th and 5th mannose residues of O-linked
glycans"
/codon_start=1
/product="Mnt2p"
/protein_id="EWG90788.1"
/translation="MRRKNRLFILVVLLGIVLVVYYSQLNSLDLVEPVQSSSSGNGGC
WSYYEGLTPGWLNDFYDVNQITPNPAKDVIELVTRIKIFFNCLQQVDGHNIQRLRDIE
KKLFPYINFEKLETDESAFWHTTTRWNGEVYHASVLEFDPKNHQFLRSKPINFDTGLS
FWENWLHTVTQSGSKGIVISASDVQLNETIRLLKVLRFIKNDYPIQIVHNADLSQDSM
KSIIKYARSLDAAEYPAQELWFLNVHSLLNPKYSKKFTTYSNKWLALTFSSFETPILM
DSDTVPFVSIEKFYELKEFQKTGVLFFKDRVISDDLFESSELEILREIIYGCIGLDLE
DESKIHEQVEDPVVAQVLENMFIKKYKHHLESGLVVLHKGKHLFSMLTSIALQFSPIA
EYFHGDKDFFWLGELLSNNRFTFHPVDASNIGRLGNVVSKENTGEFYQICSVQLSHTD
RDGSLLWLNGGLNICKKASWEYDYEHRQRLNDMFQNADELREYYASPVELEGIIIPDT
SISGWINSGECFLFNYCTLFKEGEFGKLIKFKEDEKHRLSQIVDIWNKDI"
gene 13856..15253
/gene="Adh4"
/locus_tag="P301_G10056"
mRNA 13856..15253
/gene="Adh4"
/locus_tag="P301_G10056"
/product="Adh4p"
CDS 13856..15253
/gene="Adh4"
/locus_tag="P301_G10056"
/note="Similar to YGL256W 'Alcohol dehydrogenase isoenzyme
type IV, dimeric enzyme demonstrated to be zinc-dependent
despite sequence similarity to iron-activated alcohol
dehydrogenases; transcription is induced in response to
zinc deficiency'"
/codon_start=1
/product="Adh4p"
/protein_id="EWG90789.1"
/translation="MLGITYAVNSTKQLIFCCLKYLTLLGYILLSNRKKGQRTNMYKR
VISISGLLKTGVKRFSSVYCKTTINNKFTFATTNSQIRKMSSVTGFYIPPISFFGEGA
LEETADYIKSKDYKKALIVTDPGIAAIGLSGRVQKMLEERGLNVAIYDKTQPNPNIAN
VTAGLKVLKEENSEIVVSIGGGSAHDNAKAIALLATNGGEIGDYEGVNQSKKAALPLF
AINTTAGTASEMTRFTIISNEEKKIKMAIIDNNVTPAVAVNDPSTMFGLPPALTAATG
LDALTHCIEAYVSTASNPITDACALKGIDLINESLVAAYKDGKDKKARTDMCYAEYLA
GMAFNNASLGYVHALAHQLGGFYHLPHGVCNAVLLPHVQEANMQCPKAKKRLGEIALH
CGASQEDPEETIKALHVLNRTMNIPRNLKDLGVKTEDFDILAEHAMHDACHLTNPVQF
TKEQVVAIIKKAYEY"
gene 19939..21063
/gene="Zrt1"
/locus_tag="P301_G10086"
mRNA 19939..21063
/gene="Zrt1"
/locus_tag="P301_G10086"
/product="Zrt1p"
CDS 19939..21063
/gene="Zrt1"
/locus_tag="P301_G10086"
/note="Similar to YGL255W 'High-affinity zinc transporter
of the plasma membrane, responsible for the majority of
zinc uptake; transcription is induced under low-zinc
conditions by the Zap1p transcription factor'"
/codon_start=1
/product="Zrt1p"
/protein_id="EWG90790.1"
/translation="MSNVTKPWWKQWDPDTVTLTAETPDDVWKTCVLQGVYFGENEYN
GNLGARISSVFVILFVSTFFTMFPLISTKVKRLRIPLYAYLFAKYFGSGVIVATAFIH
LMDPAYGAIGGNTCVGQTGNWGLYSWCPAIMLTSLTFTFLTDLFSSVWVERKYGLSHD
HTNDEIKDTVVNNAAVVSTENENGTANGSHDTKNGIEYFEESDATSVDVVQSFQTQFY
AFLILEFGVIFHSVMIGLNLGSTGKEFATLYPVLVFHQSFEGLGIGARLSAIEFPRSK
RWWPWALCVAYGLTTPICVAIGLGVRTRYVSGSYTALVISGVLDAISAGILLYTGLVE
LLARDFIFNPHRTKNLKELSFNVICTLFGAGIMALIGKWA"
gene 21259..22158
/gene="Fzf1"
/locus_tag="P301_G10096"
mRNA 21259..22158
/gene="Fzf1"
/locus_tag="P301_G10096"
/product="Fzf1p"
CDS 21259..22158
/gene="Fzf1"
/locus_tag="P301_G10096"
/note="Similar to YGL254W Transcription factor involved in
sulfite metabolism, sole identified regulatory target is
SSU1, overexpression suppresses sulfite-sensitivity of
many unrelated mutants due to hyperactivation of SSU1,
contains five zinc fingers"
/codon_start=1
/product="Fzf1p"
/protein_id="EWG90791.1"
/translation="MTDIGRTKSRNYKCSFDGCEKVYNRPSLLQQHQNSHTNQKPYHC
DEPGCGKKFIRPCHLRVHKWTHSQIKPKACTLCQKRFVTNQQLRRHLNSHERKSKLAS
RIDRKHEGVNANVKAELDGKEGGFDPKLPSGSPMCGEEFSQGHLPGYDDMQVLQCPYK
SCQKVTSFNDDLINHMLQHHIASKLVVPSGDPSLKESLPTSEKSSSTDTTSIPQLSFS
TTGTSSSESIDSTTAQTPTDPESYWSDNRCKHSDCQELSPFASVFDLIDHYDHTHAFI
PETLVKYSYIHLYKPSVWDLFEY"
gene 22896..24356
/gene="Hxk2"
/locus_tag="P301_G10101"
mRNA 22896..24356
/gene="Hxk2"
/locus_tag="P301_G10101"
/product="Hxk2p"
CDS 22896..24356
/gene="Hxk2"
/locus_tag="P301_G10101"
/note="Similar to YGL253W 'Hexokinase isoenzyme 2 that
catalyzes phosphorylation of glucose in the cytosol;
predominant hexokinase during growth on glucose; functions
in the nucleus to repress expression of HXK1 and GLK1 and
to induce expression of its own gene'"
/codon_start=1
/product="Hxk2p"
/protein_id="EWG90792.1"
/translation="MVHLGPKKPQARKGSMADVPKELMQQIENFEKIFTVPTETLQAV
TKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLRVVLVKLGGD
RTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQFPQGISEPIPLGFTFSFP
ASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALINDTTGTLVA
SYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGKLSDDIPPSAPMAINCEYGSFDNE
HVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDMYKQGFIFKNQDLS
KFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGINTTVQERKLIRRLSELIGARA
ARLSVCGIAAICQKRGYKTGHIAADGSVYNRYPGFKEKAANALKDIYGWTQTSLDDYP
IKIVPAEDGSGAGAAVIAALAQKRIAEGKSVGIIGA"
gene complement(24677..26443)
/gene="Rtg2"
/locus_tag="P301_G10106"
mRNA complement(24677..26443)
/gene="Rtg2"
/locus_tag="P301_G10106"
/product="Rtg2p"
CDS complement(24677..26443)
/gene="Rtg2"
/locus_tag="P301_G10106"
/note="Similar to YGL252C 'Sensor of mitochondrial
dysfunction; regulates the subcellular location of Rtg1p
and Rtg3p, transcriptional activators of the retrograde
(RTG) and TOR pathways; Rtg2p is inhibited by the
phosphorylated form of Mks1p'"
/codon_start=1
/product="Rtg2p"
/protein_id="EWG90793.1"
/translation="MSTLSDSDTETEVVSRNLCGIVDIGSNGIRFSISSKAAHHARIM
PCVFKDRVGLSLYEVQYNTHTNAKCPIPRDIIKEVCSAMKRFKLICDDFGVPETSVRV
IATEATRDAINADEFVNAVYGSTGWKVEILGQEDETRVGIYGVVSSFNTVRGLYLDVA
GGSTQLSWVISSHGEVKQSSKPVSLPYGAGTLLRRMRTDDNRALFYEIKEAYKDAIEK
IGIPQEMIDDAKKEGGFDLWTRGGGLRGMGHLLLYQSEGYPIQTIINGYACTYEEFSS
MSDYLFLKQKIPGSSKEHKIFKVSDRRALQLPAVGLFMSAVFEAIPQIKAVHFSEGGV
REGSLYSLLPKEIRAQDPLLIASRPYAPLLTEKYLYLLRTSIPQEDIPEIVNERIAPA
LCNLAFVHASYPKELQPTAALHVATRGIIAGCHGLSHRARALIGIALCSRWGGNIPES
EEKYSQELEQVVLREGDKAEALRIVWWTKYIGTIMYVICGVHPGGNIRDNVFDFHVSK
RSEVETSLKELIIDDANTTKVKEESTRKNRGYEVVVRISKDDLKTSASVRSRIITLQK
KVRKLSRGSVERVKIGVQFYEE"
gene complement(26880..30595)
/gene="Hfm1"
/locus_tag="P301_G10116"
mRNA complement(join(26880..30385,30538..30595))
/gene="Hfm1"
/locus_tag="P301_G10116"
/product="Hfm1p"
CDS complement(join(26880..30385,30538..30595))
/gene="Hfm1"
/locus_tag="P301_G10116"
/note="Similar to YGL251C 'Meiosis specific DNA helicase
involved in the conversion of double-stranded breaks to
later recombination intermediates and in crossover
control; catalyzes the unwinding of Holliday junctions;
has ssDNA and dsDNA stimulated ATPase activity'"
/codon_start=1
/product="Hfm1p"
/protein_id="EWG90794.1"
/translation="MKTKFDRLGTGKRSRPSPNNIDFNDQSATFKRNKKNSRQPSFKV
GLSYNSLLDDCDDENETEEIFEGRGLQFFDKDDNFSITADDTQVTSKLFDHDLEQTPD
EEAKKPKKVTIRKSAKKCLSTTILPDSFRGVFKFTEFNKMQSEAFPSIYESNENCIIS
SPTGSGKTVLFELAILRLIKETNSDTNNTKIIYIAPTKSLCYEMYKNWFPSFVNLSVG
MLTSDTSFLETEKAKKCNIIITTPEKWDLLTRRWSDYSRLFELVKLVLVDEIHTIKEK
RGASLEVILTRMNTMCQNIRFVALSATVPNIEDLALWLKTNNELPANILSFDESYRQV
QLTKFVYGYSFNCKNDFQKDAIYNSKLIEIIEKHADNRPVLIFCPTRASTISTAKFLL
NNHIFSKSKKRCNHNPSDKILNECMQQGIAFHHAGISLEDRTAVEKEFLAGSINILCS
TSTLAVGVNLPAYLVIIKGTKSWNSSEIQEYSDLDVLQMIGRAGRPQFETHGCAVIMT
DSKMKQTYENLIHGTDVLESSLHLNLIEHLAAETSLETVYSIETAVNWLRNTFFYVRF
GKNPAAYQEVNRYVSFHSVEDSQINQFCQYLLDTLVKVKIIDISNGEYKSTAYGNAMT
RHYISFESMKQFINAKKFLSLQGILNLLATSEEFSVMRVRHNEKKLFKEINLSPLLKY
PFLTEKKQSQIIDKVSQKVSLLIQYELGGLEFPSYEGASKLHQTLVQDKFLVFRHCFR
LLKCMVDTFIEKSDGTSLKNTLFLLRSLNGHCWENTPMVLRQLKTIGLVSVRRLIRHG
ITNLEEMGHLSDTQIEYYLNLKIGNGIKIKNDISLLPCLNIRTKLENCKIENEELWLT
FKVEISATFKSSIWHGQHLSLDIETEKSSGELIDFRRLQLNKLQSPRGFRISAKISPK
LEKIEFSIHCQEIAGLGKTIVYSTDHLASQFSAKTPNIRKDLNSLEKCLFYESSSDGE
VGKTSRVSHKDGLEESLSSDDSILDYLNERKKSSKAVESAAVIHPEAHSSSHFSNGRQ
VRSNGNYECFHSCKDKTQCRHLCCKEGIPVKYIKEKGPSSIKPVSKPDQIRQPLLAKN
INTTPHLEKRLNSKPKQWQEENTDIATVHTLPSKIYNLSQQMSSMEAGEQVLKSGPEN
CPEIIPIDLESSDSYSSNTAASSISDPNGDLDFLGSDIEFE"
gene 30869..31594
/gene="Rmr1"
/locus_tag="P301_G10121"
mRNA 30869..31594
/gene="Rmr1"
/locus_tag="P301_G10121"
/product="Rmr1p"
CDS 30869..31594
/gene="Rmr1"
/locus_tag="P301_G10121"
/note="Similar to YGL250W 'Protein required for meiotic
recombination and gene conversion; null mutant displays
reduced PIS1 expression and growth defects on
non-fermentable carbon sources and minimal media;
GFP-fusion protein localizes to both cytoplasm and
nucleus'"
/codon_start=1
/product="Rmr1p"
/protein_id="EWG90795.1"
/translation="MSEEEAHKTVEVDDVGVQLDEGDEEDLLEYDDELVEEQPSDARI
RNVAETLMKSELPKVTVEYKDTTFLLFTSDDKNESNNPIICENAALYQRPMGEFMESI
RKFMGNRFGRLAFATKELVLQLKSLDLTLFEDNVYNNHISFSDVYTIFKILKERSESN
FETDIPTHLAIELSTRPRFVSRYNALVELTESSATLKNIKPFSNDETHPLIVDDNDQY
THQNTSEVIVMDIDDDVGEDSED"
gene 32056..34061
/gene="Zip2"
/locus_tag="P301_G10131"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 34611..35720
/gene="Pde1"
/locus_tag="P301_G10141"
mRNA 34611..35720
/gene="Pde1"
/locus_tag="P301_G10141"
/product="Pde1p"
CDS 34611..35720
/gene="Pde1"
/locus_tag="P301_G10141"
/note="Similar to YGL248W 'Low-affinity cyclic AMP
phosphodiesterase, controls glucose and intracellular
acidification-induced cAMP signaling, target of the
cAMP-protein kinase A (PKA) pathway; glucose induces
transcription and inhibits translation'"
/codon_start=1
/product="Pde1p"
/protein_id="EWG90796.1"
/translation="MVVFEITILGANGGPTEYGTQCFILKPARTEDPELIAVDGGAGM
YQLREMLVQGRNENEGDDELVPSFYEHDREPIEFFIDSKLNIQKGLSKSLLQSLKRQG
EHFESANTMKKTYEVFQGITDYYITHPHLDHISGLVVNSPSIYEQENSKKKTIWGLPH
TIDVLQKHVFNDLIWPDLTAERSRKLKLKCLNPKEVQKCTIFPWDVIPFKVHHGIGVK
TGAPVYSTFYIFRDRKSKDCIIVCGDVEQDRRESEESLLEEFWSYVAENIPLVHLKGI
LVECSCPLSSKPEQLYGHLSPIYLINELSNLNTLYNSSKGLSGLNVIVTHVKSTPAKR
DPRLTILEELRFLAEERNLGDLRISIALEGHTLFL"
gene 35891..36484
/gene="Brr6"
/locus_tag="P301_G10146"
mRNA 35891..36484
/gene="Brr6"
/locus_tag="P301_G10146"
/product="Brr6p"
CDS 35891..36484
/gene="Brr6"
/locus_tag="P301_G10146"
/note="Similar to YGL247W 'Essential nuclear envelope
integral membrane protein required for nuclear envelope
morphology, nuclear pore complex localization, nuclear
export; exhibits synthetic lethal genetic interactions
with genes involved in lipid metabolism'"
/codon_start=1
/product="Brr6p"
/protein_id="EWG90797.1"
/translation="MELRSFSRQPDGILANPRLGREEVLEGEHPQDARLARQSIWLSP
SLIAEYIQLFFNFIIGTIGLSLAIKFILMIRNDVNLKLEHNVREELDKIATCKSRYFE
NQCEPHMRVPALEVRCNEWSKCMNKEIVSGSDYQWAKAWARTLAEVINAFFEAFSIRS
FLFILISIIGIIFVTNTSFGSYRVYLNNKDTKSVRHA"
gene complement(36575..37737)
/gene="Rai1"
/locus_tag="P301_G10151"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 37980..40106
/gene="Gus1"
/locus_tag="P301_G10156"
mRNA 37980..40106
/gene="Gus1"
/locus_tag="P301_G10156"
/product="Gus1p"
CDS 37980..40106
/gene="Gus1"
/locus_tag="P301_G10156"
/note="Similar to YGL245W 'Glutamyl-tRNA synthetase
(GluRS), forms a complex with methionyl-tRNA synthetase
(Mes1p) and Arc1p; complex formation increases the
catalytic efficiency of both tRNA synthetases and ensures
their correct localization to the cytoplasm'"
/codon_start=1
/product="Gus1p"
/protein_id="EWG90798.1"
/translation="MPSTLTINGKAPIVAYAELIAARIVNALAPNSIAIKLVDDKKAP
AAKLDDATEDVFNKITSKFAAIFDNGDKEQVAKWVNLAQKELVIKNFAKLSQSLETLD
SQLNLRTFILGGLKYSAADVACWGALRSNGMCGSIIKNKVDVNVSRWYTLLEMDPIFG
EAHDFLSKSLLELKKSANVGKKKETHKANFEIDLPDAKMGEVVTRFPPEPSGYLHIGH
AKAALLNQYFAQAYKGKLIIRFDDTNPSKEKEEFQDSILEDLDLLGIKGDRITYSSDY
FQEMYDYCVQMIKDGKAYCDDTPTEKMREERMDGVASARRDRSVEENLRIFTEEMKNG
TEEGLKNCVRAKIDYKALNKTLRDPVIYRCNLTPHHRTGSTWKIYPTYDFCVPIVDAI
EGVTHALRTIEYRDRNAQYDWMLQALRLRKVHIWDFARINFVRTLLSKRKLQWMVDKD
LVGNWDDPRFPTVRGVRRRGMTVEGLRNFVLSQGPSRNVINLEWNLIWAFNKKVIDPI
APRHTAIVNPVKIHLEGSEAPQEPKIEMKPKHKKNPAVGEKKVIYYKDIVVDKDDADV
INVDEEVTLMDWGNVIITKKNDDGSMVAKLNLEGDFKKTKHKLTWLADTKDVVPVDLV
DFDHLITKDRLEEDESFEDFLTPQTEFHTDAIADLNVKDMKIGDIIQFERKGYYRLDA
LPKDGKPYVFFTIPDGKSVNKYGAKK"
gene 40455..42131
/gene="Rtf1"
/locus_tag="P301_G10161"
mRNA 40455..42131
/gene="Rtf1"
/locus_tag="P301_G10161"
/product="Rtf1p"
CDS 40455..42131
/gene="Rtf1"
/locus_tag="P301_G10161"
/note="Similar to YGL244W 'Subunit of the RNA polymerase
II-associated Paf1 complex; directly or indirectly
regulates DNA-binding properties of Spt15p and relative
activities of different TATA elements; involved in
telomere maintenance'"
/codon_start=1
/product="Rtf1p"
/protein_id="EWG90799.1"
/translation="MSDLDEDLLALAGADESEEEDQVLTTTSAKRAKNNDQSLSKKRR
IEVGSVEDDDEEDDYNPYSVGNADYGSEEEEEANPFPLEGKYKDESDREHLESLPEME
RETLLFERSQIMQKYQERKLFRARGRDMKEQQQRAKNDEDSRKTRASTRSTHATGHSD
IKASKLSQLKKQRARKNRHYSDNEDEDDEEDYREEDYKDDEGSEYGDDEEYNPFDRRD
TYDKREEVEWAEEEDEQDREPEISDFNKLRIGRSFVAKFCFYPGFEDAVKGCYGRVNV
GTDKRTGKTSYRMVRIERVFLQKPYNMGKFYTNQYFGVTQGKDRKVFQMNYFSDGLFA
EDEYQRYLRALDNSQMIKPSLHSLSNKTKEVMDFVNTPLTDKTTDEVVRHRMQFNKKL
SGTNAVLEKTVLREKLQYAKETNNEKDIAKYSAQLRNFEKRMSVYEKHHENDQSDIKK
LGELTSKNRKLNMSNIRNAEHVKKEDSNNFDSKSDPFSRLKTRTKVYYQEIQKEENAK
AKEIAQQEKLQEDKDAKDKREKELLVAQFRRLGGLERMVGELDIKFDLKF"
gene 42310..43464
/gene="Tad1"
/locus_tag="P301_G10166"
mRNA 42310..43464
/gene="Tad1"
/locus_tag="P301_G10166"
/product="Tad1p"
CDS 42310..43464
/gene="Tad1"
/locus_tag="P301_G10166"
/note="Similar to YGL243W tRNA-specific adenosine
deaminase, deaminates adenosine-37 to inosine in tRNA-Ala"
/codon_start=1
/product="Tad1p"
/protein_id="EWG90800.1"
/translation="MPSEDKLGEEISTRVVNEYSKLKSACRPIIRPSGIREWTILAGV
AAINRDGGANKIEILSIATGVKALPDSELQRSEGKILHDCHAEILALRGANTVLLNRI
QNYNPSSGDKFIQHNDEIPARFNLKENWELALYISRLPCGDASMTFLNDNCKNDDFIK
IEDSDEFQYVDRSVKTILRGRLNFNRRNVVRTKPGRYDSNITLSKSCSDKLLMKQRSS
VLNCLNYELFEKPVFLKYIVIPNLEDETKHYLEQSFHTRLPNLDNEIKFLNCLKPFYD
DKLDEEDVPGLMCSVKLFMDDFSTEEAILNGVRNGFYTKSSNPLRKHCQSQVSRFAQW
ELFKKIRPEYEGISYLEFKSRQKKRSQLIIAIKNILSPDGWIPTRTDDVK"
gene complement(43609..44154)
/locus_tag="P301_G10171"
mRNA complement(43609..44154)
/locus_tag="P301_G10171"
/product="hypothetical protein"
CDS complement(43609..44154)
/locus_tag="P301_G10171"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90801.1"
/translation="MNTEGASLSEQLLDAARRNNLDLLETVFDSLDNDPEKIAKLINE
SKEPLGNTALHLCCKYGSWEVFDRILDQDGEIEIDPQNDVDGDTPLHVTVRYSQEEPE
HGTFIARNLIEVGADPRVRNYNNQKPVDLVHGDELDELIDLLQGAELAIDSTNGSGDN
NEDGEMIDDGPSDDDEEDDKK"
gene 44400..47414
/gene="Kap114"
/locus_tag="P301_G10176"
mRNA 44400..47414
/gene="Kap114"
/locus_tag="P301_G10176"
/product="Kap114p"
CDS 44400..47414
/gene="Kap114"
/locus_tag="P301_G10176"
/note="Similar to YGL241W 'Karyopherin, responsible for
nuclear import of Spt15p, Sua7p, histones H2A and H2B, and
Nap1p; amino terminus shows similarity to those of other
importins, particularly Cse1p; localization is primarily
nuclear'"
/codon_start=1
/product="Kap114p"
/protein_id="EWG90802.1"
/translation="MDINELIIGAQSADKHTREVAETQLLQWCDSDASQVFKALANVA
LQHEAFLESRQFALLSLRKLITMYWSPGFESYRSTSNVEVDVKDFIREVLLKLCLNDN
ENTKIKNGASYCIVQISAVDFPDQWPQLLTVIYDAISHQHSLNAMSLLNEIYDDVVSE
EMFFEGGIGLATMQIVFKVLNTETSTLIAKIAALKLLKACLLQMSSHNEYDEASRKSF
VSQCLATSLQILGQLLTLNFGNVDVISQLKFKSIIYENLVFIKNDFSRKHFSSELQKQ
FKIMAIQDLENVTHINANVETTESEPLLETVHDCSIYIVEFLTSVCTLQFSVEEMNKI
ITSLTILCQLSSETREIWTSDFNTFVSKETGLAASYNVRDQANEFFTSLPNPQLSLIF
KVVSSDIEHSTCNYSTLESLLYLLQCILLNDDEITGENIDQSLQILIKTLENILVSQE
IPELILARAILTIPRVLDKFIDALPDIKPLTSAFLAKSLNLALKSDKELIKSATLIAF
TYYCYFAELDSVLGPEVCSKTQEKVIRIINQVSSDAEEDTNGALMEVLSQVISYNPKE
PHSRKEILQAEFHLVFTISSEDPANVQVVVQSQECLEKLLDNINMDNYKNYIELCLPS
FINVLDSNNANNYRYSPLLSLVLEFITVFLKKKPNDGFLPDEINQYLFEPLAKVLAFS
TEDETLQLATEAFSYLIFNTDTRAIEPRLMDIMKVLERLLSLEVSDSAAMNVGPLVVA
IFTRFSKEIQPLIGRILEAVVVRLIKTQNISTEQNLLSVLCFLTCNDPKQTVDFLSSF
QIDNTDALTLVMRKWIEAFEVIRGEKRIKENIVALSNLFFLNDERLQKVVVNGDLIPY
EGDLIITRSMAKKMPDRYVQVPLYTKIIKLFVSELSFQSKQPNPEQLITSDIKQEVVN
ANKDDDNDDWEDVDDVLDYDKLKEYIDDDVDEEADDDSDDITGLMDVKESVVQLLVRF
FKEVASKDVSGFHCIYETLSDSERKVLSEALL"
gene 47568..48320
/gene="Doc1"
/locus_tag="P301_G10181"
mRNA 47568..48320
/gene="Doc1"
/locus_tag="P301_G10181"
/product="Doc1p"
CDS 47568..48320
/gene="Doc1"
/locus_tag="P301_G10181"
/note="Similar to YGL240W 'Processivity factor required
for the ubiquitination activity of the anaphase promoting
complex (APC), mediates the activity of the APC by
contributing to substrate recognition; involved in cyclin
proteolysis'"
/codon_start=1
/product="Doc1p"
/protein_id="EWG90803.1"
/translation="MDPIGINKVLDHLAPSELIKPVKSCHNKPSVLVLDDRIVDAATK
DLYVNGFQEEIQYQNPTPENLQDMFHQGIEIMDSARMINVTHLALWKPSSFKLGNPVD
FALDDNYDTFWQSDGGQPHQLDIMFSKRMDICVMAIFFSMIADESYAPSLVKVYAGHS
PSDARFYKMLEVRNVNGWVALRFLDNREDDQLLKCQFIRLLFPVNHENGKDTHLRGIR
LYVPSNEPHQDTHEWAQTLPETNNVFQDAILR"
gene 48506..51389
/gene="Cse1"
/locus_tag="P301_G10191"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(51682..52482)
/gene="Hap2"
/locus_tag="P301_G10196"
mRNA complement(51682..52482)
/gene="Hap2"
/locus_tag="P301_G10196"
/product="Hap2p"
CDS complement(51682..52482)
/gene="Hap2"
/locus_tag="P301_G10196"
/note="Similar to YGL237C 'Subunit of the heme-activated,
glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a
transcriptional activator and global regulator of
respiratory gene expression; contains sequences sufficient
for both complex assembly and DNA binding'"
/codon_start=1
/product="Hap2p"
/protein_id="EWG90804.1"
/translation="MSADETDAKFHPLETDLQSDTAAAISTAAASRSPSLREKPTEMP
LDMGKAPSPRGEDQRVTNEEDLFLFNRLRASQNRVMDSLEPQQQSQYTSSSVSTMEPS
ADFASFSAVTTLPPPPHQQQQQQRQQQQQQQQLVVQAQYTQNQPNLQSDVLGTAIAEQ
PFYVNAKQYYRILKRRYARAKLEEKLRISRERKPYLHESRHKHAMRRPRGEGGRFLTA
AEIKAMKSKKSGASDDPDDSHEDKKITTKIIQEQPHATSTAAAADNKT"
gene complement(52740..54749)
/gene="Mto1"
/locus_tag="P301_G10201"
mRNA complement(52740..54749)
/gene="Mto1"
/locus_tag="P301_G10201"
/product="Mto1p"
CDS complement(52740..54749)
/gene="Mto1"
/locus_tag="P301_G10201"
/note="Similar to YGL236C 'Mitochondrial protein, forms a
heterodimer complex with Mss1p that performs the
5-carboxymethylaminomethyl modification of the wobble
uridine base in mitochondrial tRNAs; required for
respiration in paromomycin-resistant 15S rRNA mutants'"
/codon_start=1
/product="Mto1p"
/protein_id="EWG90805.1"
/translation="MLRVTTLASSCTSFPLQVLRRRLTISSLTSFQPTTKTQVVVIGA
GHAGCEAAAASSRTGAHTTLITPSLTDIGKCSCNPSIGGVGKGILVKEIDALDGLMGK
VTDLAGVQFKMLNRSKGPAVWGPRAQIDRELYKKYMQRELSDKKAHPNLSLLQNKVAD
LILYDPGCGHKVIKGVVLDDGTQVGADQVIITTGTFLSAEIHIGDKRIAAGRIGEQPT
YGISNTLQNEVGFQLGRLKTGTPARLAKESIDFSALEVQKGDKLPVPMSFLNETVSVE
PTKQLDCFGTHTTPQMHDFLRNNLHQSIHIQDTTIKGPRYCPSIEAKILRFPDRSSHK
IWLEPEGFNSDVIYPNGISNSMPEDVQLQMMRLIPGMANVEILQPAYGVEYDYVDPRQ
LKPSLETKLVDGLFLAGQINGTTGYEEAAAQGIIAGINAGLLSRQEREQLVLKRSQAY
IGVLIDDLINNGVIEPYRMFTSRSEFRISVRADNADFRLTPIGAQLGIISPVRLSQYS
RDKHLYDETIRALQNFKLSSQKWSSLLQANIAPQAENRSAWEIFRFKDMDLHKLYECI
PDLPINLLDIPMHVVTKINIQGKYEPYIVKQNQFVKAFQADENMLLPQDYDYRQLPTL
STECKLLLNRVQPLTIGQARRIQGITAAALFELYRVARKPSQPVM"
gene 54232..54768
/locus_tag="P301_G10206"
mRNA 54232..54768
/locus_tag="P301_G10206"
/product="hypothetical protein"
CDS 54232..54768
/locus_tag="P301_G10206"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90806.1"
/translation="MTLWPHPGSYKIKSATLFCSRDKLGCAFLSESSLCMYFLYNSLS
IWALGPHTAGPLLLLSILNCTPARSVTLPISPSRASISFTRMPLPTPPIEGLHEHLPI
SVNDGVMRVVCAPVLDDAAAASQPACPAPMTTTCVLVVGWKLVKEDMVNRLLRTCKGN
EVHEDAKVVTRSMVLWGV"
gene 55435..57843
/gene="Ade5,7"
/locus_tag="P301_G10211"
mRNA 55435..57843
/gene="Ade5,7"
/locus_tag="P301_G10211"
/product="Ade5,7p"
CDS 55435..57843
/gene="Ade5,7"
/locus_tag="P301_G10211"
/note="Similar to YGL234W Bifunctional enzyme of the 'de
novo' purine nucleotide biosynthetic pathway, contains
aminoimidazole ribotide synthetase and glycinamide
ribotide synthetase activities"
/codon_start=1
/product="Ade5,7p"
/protein_id="EWG90807.1"
/translation="MLNILVLGNGAREHVLVTKLAQSPTVGKIYVAPGNGGTATMDPS
RVINWDITPDVANFARLQSMAVEHKINLVVPGPELPLVNGITSVFHSVGIPVFGPSVK
AAQLEASKAFSKRFMSKHNIPTASYDVFTNPEEAISFLQAHTDKAFVIKADGIAAGKG
VIIPSSIDESVQAIKDIMVTKQFGEEAGKQVVIEQFLEGDEISLLTIVDGYSHFNLPV
AQDHKRIFDGDKGLNTGGMGAYAPAPVATPSLLKTIDSQIVKPTIDGMRRDGMPFVGV
LFTGMILVKDSKTNQLVPEVLEYNVRFGDPETQAVLSLLDDQTDLAQVFLAAAEHRLD
SVNIGIDDTRSAVTVVVAAGGYPESYAKGDKITLDTDKLPPHTQIFQAGTKYDSATDS
LLTNGGRVLSVTSTAQDLRTAVDTVYEAVKCVHFQNSYYRKDIAYRAFQNSESSKVAI
TYADSGVSVDNGNNLVQTIKEMVRSTRRPGADSDIGGFGGLFDLAQAGFRQNEDTLLV
GATDGVGTKLIIAQETGIHNTVGIDLVAMNVNDLVVQGAEPLFFLDYFATGALDIQVA
SDFVSGVANGCIQSGCALVGGETSEMPGMYPPGHYDTNGTAVGAVLRQDILPKINEMA
AGDVLLGLASSGVHSNGFSLVRKIIQHVALPWDAPCPWDESKTLGEGILEPTKIYVKQ
LLPSIRQRLLLGLAHITGGGLVENIPRAIPDHLQARVDMSTWEVPRVFKWFGQAGNVP
HDDILRTFNMGVGMVLIVKRENVKAVCDSLTEEGEIIWELGSLQERPKDAPGCVIENG
TKLY"
gene 58075..60807
/gene="Sec15"
/locus_tag="P301_G10221"
mRNA 58075..60807
/gene="Sec15"
/locus_tag="P301_G10221"
/product="Sec15p"
CDS 58075..60807
/gene="Sec15"
/locus_tag="P301_G10221"
/note="Similar to YGL233W 'Essential 113kDa subunit of the
exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p,
Sec15p, Exo70p, and Exo84p), which mediates polarized
targeting of vesicles to active sites of exocytosis;
Sec15p associates with Sec4p and vesicles'"
/codon_start=1
/product="Sec15p"
/protein_id="EWG90808.1"
/translation="MDQEGQPLLSKDFQQVLLATASGNNSSWTERAVLNNESTDAVKH
EPALGQNDVFDLDPLSFDKWVPFLRRALDKNQLDPVIDELENSIEDNFQGLELQLLQD
SQMNDKLETSIDEIANIQGMVQDTLSSEISKFQIRLSESANELIVKKQMYVNNKKISL
KISEATILITKVVRILELSSKCQELITERKFFKVLQNLDSLEKLYLQEFKNYNFQFLI
EIYNSIPFLQKVTKDECINLIRNSLNLNLGKNLIKVGQEFVAIYENELLPQWLETRSK
MKLTNFKFNSPIEISMRDESFLAKLNLGEFFQLDDFHDSIMIFQNLNELSVLSGEFNK
EYELRKTKLMYPLIWKKNKTAAYQMDSLLRGTGTTPGSTAHDVSTDDPFTQSLSLHFL
QDYFLKILGFLLYDINLNKATEFILVDNNYNSTNEFWDGLMDRLSPYLSYFIDEKLKT
EEDMIKLKDFLCIYVAILENFKLNIEPLYKILVSIFEKFCSVSLRAFDDEFQILLNDD
DFMPLSINDKTLYEKVLKICWMKEGEHLSLPDPTNGEPFAVTLPFSPLYPMTCTLAKK
TYSKITAFLSIFYRHELHTLNNILVKTMDDIFNDIVNKKIRSKLESTSREEIAQILVN
LDYFIIAAKEFSNFMTRENILQNPDMEIRLSSIKYLAESRKLAETKLIELIDSKISDI
LETIEIDWQITEVRQDPDISIIDLAQFLEMMFASTLQNLPYSVQTLLIFREFDSLTRQ
FMGLLLHDTPSTITHESIMNFEVDVNYLESIIPRIFPSTPGTIDSNGYQSPXXXXXXX
XXNANGVDAPTLFENNIKSLEATFMELKQCIELLKTQGKDYNEPEIRLRKYSRIRQED
AALLLSKIQHFVSSVEGANGDDTSVMDSSSIFNSESASVIDSNTSRIAKFFNRR"
assembly_gap 60447..60474
/estimated_length=28
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 61030..61957
/gene="Tan1"
/locus_tag="P301_G10226"
mRNA join(61030..61086,61145..61957)
/gene="Tan1"
/locus_tag="P301_G10226"
/product="Tan1p"
CDS join(61030..61086,61145..61957)
/gene="Tan1"
/locus_tag="P301_G10226"
/note="Similar to YGL232W Putative tRNA acetyltransferase,
RNA-binding protein required for the formation of the
modified nucleoside N(4)-acetylcytidine in serine and
leucine tRNAs but not required for the same modification
in 18S rRNA"
/codon_start=1
/product="Tan1p"
/protein_id="EWG90809.1"
/translation="MGEKRNRNGKDANSQNRKKFKVSSGFLDPGTSGIYATCSRRHER
QAAQELQLLFEEKFQELYGDIKEGEDESENDEKKDLSIEDQIKKELQELKGEETGKDL
SSGETKKKDPLAFIDLNCECVTFCKTRKPIVPEEFVLSIMKDLADPKNMVKRTRYVQK
LTPITYSCNAKMEQLIKLANLVIGPHFHDPSNVKKNYKFAVEVTRRNFNTIERMDIIN
QVVKLVNKEGSEFNHTVDLKNYDKLILVECFKSNIGMCVVDGDYKTKYRKYNVQQLYE
SKFRKDEDKSVKQ"
gene complement(62003..62575)
/locus_tag="P301_G10231"
mRNA complement(62003..62575)
/locus_tag="P301_G10231"
/product="hypothetical protein"
CDS complement(62003..62575)
/locus_tag="P301_G10231"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90810.1"
/translation="MSEQEPYEWAKHLLDTKYIEKYNIQNSNTLPSPPGFEGNSSKGN
VTRKQQDATSQTTSLAQKNQITVLQVQKAWQIALQPAKSIPMNIFMSYMSGTSLQIIP
IMTALMLLSGPIKAIFSTRSAFKPVLGNKATQSQVQTAMFMYIVFQGVLMYIGYRKLN
SMGLIPNAKGDWLPWERIAHYNNGLQWFSD"
gene complement(62727..63170)
/locus_tag="P301_G10241"
mRNA complement(62727..63170)
/locus_tag="P301_G10241"
/product="hypothetical protein"
CDS complement(62727..63170)
/locus_tag="P301_G10241"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90811.1"
/translation="MGIITLSGNVLHLLKAYPKKGLEEVSQPEPNTANDSSTEYKGKS
KDDFQMVEKSNTDERYNFTRTKKWFLLMTSEYYKLMENRLLMFCIIACSFICAIQFLF
FIIYWTNIVPRKTQRAITNLNYDYLTAHLKEQCVPYAKILDQCIL"
gene complement(63458..65914)
/gene="Sap4"
/locus_tag="P301_G10246"
mRNA complement(63458..65914)
/gene="Sap4"
/locus_tag="P301_G10246"
/product="Sap4p"
CDS complement(63458..65914)
/gene="Sap4"
/locus_tag="P301_G10246"
/note="Similar to YGL229C Protein required for function of
the Sit4p protein phosphatase, member of a family of
similar proteins that form complexes with Sit4p, including
Sap155p, Sap185p, and Sap190p"
/codon_start=1
/product="Sap4p"
/protein_id="EWG90812.1"
/translation="MSLWPFGETLSHSGIDSILEEYYLIFRSLEGNETSSTDDKKNEP
SMESESEFGTESRDRSDLNQSFIDRILLETALLDELNGGANDRLVDFICLGYFYDDRS
QQVRHMDYLVDMLMAYLKDIDRTGYRTPFLLENSFHQTGEYEDQDDEDPMLYVNIISS
IFCSKSAPIVEALVQNTPFLSSLFEVFQFENIEAENCPILAVFLKINETLLFEQTSSY
LEFFKSQPNIVDKFLYHIEVSPLVEFLIKIMLTDQVESPTNIIDFLYHQDLIPKCLNL
LDNSKYSPGIQNSSGELLKALISISTNFKLDTLWIGPNRLTRQLASPQYVDQLINIIL
FQRGHAMGVAVSIIIELIRKNNSDYDEVDLLSTTIVDNPPSQRDPVYLGHLLYELTMH
MEDFYALLIKLENDDDDDHDTASKALPSVKHHLLENQLHESFRPLGFERVKITELISE
MLHCSNMGLMNSKRGEKIARTRDKCRDTLDQNSLEKAMKNLNIXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXI
RKNPTIGDLFKIKLHDLGFFPKFLQLFLRYPWNNFWHNIVFDIIQQIFNGRMDFSYNS
FLVYSLFDFKKSTRFIPKPLYGSNQKLPVKDFHIISDFILQGHKDSFEFYEKEKTNLG
YMGQLVLIAEEIAKYSKIYKTDLIAPDIYAFLQDEVWMSYSSDILNETRTMCSIILGG
GQFCAESDENTNQDFLEKADMSKPAHPSTMDENEIVHEEDVKLHDKVAELIDELGQLT
ELDIHDKIKDVIVDHHSDLN"
assembly_gap 64222..64433
/estimated_length=212
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 66553..68286
/gene="She10"
/locus_tag="P301_G10251"
mRNA 66553..68286
/gene="She10"
/locus_tag="P301_G10251"
/product="She10p"
CDS 66553..68286
/gene="She10"
/locus_tag="P301_G10251"
/note="Similar to YGL228W 'Putative
glycosylphosphatidylinositol (GPI)-anchored protein of
unknown function; overexpression causes growth arrest'"
/codon_start=1
/product="She10p"
/protein_id="EWG90813.1"
/translation="MGKLIKLITTLTVLVSLLQYCCEFNSGSISCERTQTLCHYTNPR
VWNTYFSRNCELYKNKVSPGFDIVARKYDTAVKPVIDDATVKVNKVAIQPAFKVIHSQ
CKKWNCGKYYQLVRSPMVKTRRFFFAKYNAFVKPNIDKFFTAEFRSHLKERILKCKNI
GHYYFTITSRCIKSKYDFIVGNTEEKLMGKFKNKDTHGIHGSVTHEPSSEDMVLTVST
MESDEEELTTTSTQTVVETITLDQEEASAVANHAHDDEASTDVEDSTDVNVNEQALLQ
EDFDMWSETILQKTQDVIQLFEKDVSKYINGKLVEEANHFKAKFQSLDDKSKKFFSKI
SLAINDIECVEGIDSETGKKIFFDKSGSTEISQYITRELVREYFNETRSTLDELTNAM
EKDLSEITDEIEKKVNAIREENVEVFEEWGDIIVNEWSKRMAYVDVINAHMGADDDTT
LDEEKAKSSVNWKKFLKGKKQIIESRDKLAHHSADLSRVNAFRQKVQKKILSFTQESG
EFLYILRSKANLQFQERERKERERKEREKAAAEEFQRQQELLLQQEEEDEEDVSYTST
STITTTTTMTL"
gene 68636..71512
/gene="Vid30"
/locus_tag="P301_G10261"
mRNA 68636..71512
/gene="Vid30"
/locus_tag="P301_G10261"
/product="Vid30p"
CDS 68636..71512
/gene="Vid30"
/locus_tag="P301_G10261"
/note="Similar to YGL227W 'Protein involved in
proteasome-dependent catabolite degradation of
fructose-1,6-bisphosphatase (FBPase); binds FBPase; shifts
the balance of nitrogen metabolism toward glutamate
production; localizes to the nucleus and the cytoplasm'"
/codon_start=1
/product="Vid30p"
/protein_id="EWG90814.1"
/translation="MSEYMDDVDREFINCLFPSYLLQQPVAYDLWILYLQHRKLFHKL
KNTNLINADENPTGVGMGRTKLTALTRKEIWSKLMNLGVLGTISFEAVNDDYLIQVYK
YFYPDVNDFTLRFGVKDSNKNSVRVMKASSDMRKNAQELLEPVLSEREMALNSNTSLE
NDRNDDDDDDDDDDDDDDDDDDDDDESDLESLEGEVDTDTDDNNEGDGSDNHEEGGEE
GSRGADADVSSAQQRAERVADPWIYQRSRLAINIETESRNLWDTSDKNSGLQYYPPDQ
SPSSSFSSPRVSSGNDKNDNEATNVLSNSGSKKKNSMIPDIYKILGYFLPSRWQAQPN
NSLQLSQDGITHLQPNPDYHSYMTYERSSASSASTRNRLRTSFENSGKVDFAVTWANK
SLPDNKLTIFYYEIKVLSVTSTESAENSNIVIGYKLVENELMEATTKKSVSRSSVAGS
SSSLGGSNNMSSNRVPSTSFTMEGTQRRDYIYEGGVSAMSLNVDGSINKCQKYGFDLN
VFGYCGFDGLITNSTEQSKEYAKPFGRDDVIGCGINFIDGSIFFTKNGIHLGNAFTDL
NDLEFVPYVALRPGNSIKTNFGLNEDFVFDIIGYQDKWKSLAYEHICRGRQMDVSIEE
FDSDESEEDETENGPEENKSTNVNEDLMDIDQEDGAAGNKDTKKLNDEKDNNLKFLLG
EDNRFIDGKLVRPDVNNINNLSVDDGSLPNTLNVMINDYLIHEGLVDVAKGFLKDLQK
DAVNVNGQHSESKDVIRHNERQIMKEERMVKIRQELRYLINKGQISKCINYIDNEIPD
LLKNNLELVFELKLANYLVMIKKSSSKDDDEIENLILKGQKLSNEFIYDTKIPQSLRD
RFSGQLSNVSALLAYSNPLVEAPKEISGYLSDEYLQERLFQVSNNTILTFLHKDSECA
LENVISNTRAMLSTLLEYNAFGSTNSSDPRYYKAINFDEDVLNL"
gene complement(71714..72123)
/gene="Ost5"
/locus_tag="P301_G10266"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 72303..72674
/locus_tag="P301_G10271"
mRNA 72303..72674
/locus_tag="P301_G10271"
/product="hypothetical protein"
CDS 72303..72674
/locus_tag="P301_G10271"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90815.1"
/translation="MMGRNGILLALKRSFSTYQPPVVEITNITKLWPTLRPEVRDEIK
EYLRWRMEEDWRHIPLEETKAAYFLSYGPCGGRSKGNEWNVGYTGMRMVFNLVLFGGA
ATAFYNWKQDKKLEEQLRDLV"
gene complement(72791..72864)
/locus_tag="P301_G10276"
tRNA complement(72791..72864)
/locus_tag="P301_G10276"
/product="tRNA-Val"
/note="TV(AAC)G3"
assembly_gap 72880..73129
/estimated_length=250
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 75861..76874
/gene="Vrg4"
/locus_tag="P301_G10291"
mRNA 75861..76874
/gene="Vrg4"
/locus_tag="P301_G10291"
/product="Vrg4p"
CDS 75861..76874
/gene="Vrg4"
/locus_tag="P301_G10291"
/note="Similar to YGL225W 'Golgi GDP-mannose transporter;
regulates Golgi function and glycosylation in Golgi'"
/codon_start=1
/product="Vrg4p"
/protein_id="EWG90816.1"
/translation="MSELKTGHAGHNPWASVANSGPISILSYCGSSILMTVTNKFVVN
LKDFNMNFVMLFVQSLVCTITLIILRILGYAKFRSLNKTDAKNWFPISFLLVLMIYTS
SKALQYLAVPIYTIFKNLTIILIAYGEVLFFGGSVTSMELSSFLLMVLSSVVATWGDQ
QAVAAKAASLAEGAAGAVASFNPGYFWMFTNCITSALFVLIMRKRIKLTNFKDFDTMF
YNNVLALPILLLFSFCVEDWSSVNLTNNFSNDSLTAMIISGVASVGISYCSGWCVRVT
SSTTYSMVGALNKLPIALSGLIFFDAPRNFLSILSIFIGFLSGIIYAVAKQKKQQAQP
LRK"
gene complement(76979..77821)
/gene="Sdt1"
/locus_tag="P301_G10296"
mRNA complement(76979..77821)
/gene="Sdt1"
/locus_tag="P301_G10296"
/product="Sdt1p"
CDS complement(76979..77821)
/gene="Sdt1"
/locus_tag="P301_G10296"
/note="Similar to YGL224C 'Pyrimidine nucleotidase;
overexpression suppresses the 6-AU sensitivity of
transcription elongation factor S-II, as well as
resistance to other pyrimidine derivatives'"
/codon_start=1
/product="Sdt1p"
/protein_id="EWG90817.1"
/translation="MTVEYTASDLATYQNEVNEQIAKNKAHLESLTHPGSKVTFPIDQ
DISATPQNPNLKVFFFDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNS
YYKEYGLAIRGLVMFHKVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDK
LWLFTNAYKNHAIRCLRLLGIADLFDGLTYCDYSRTDTLVCKPHVKAFEKAMKESGLA
RYENAYFIDDSGKNIETGIKLGMKTCIHLVENEVNEILGQTPEGAIVISDILELPHVV
PDLF"
gene complement(78057..79310)
/gene="Cog1"
/locus_tag="P301_G10301"
mRNA complement(78057..79310)
/gene="Cog1"
/locus_tag="P301_G10301"
/product="Cog1p"
CDS complement(78057..79310)
/gene="Cog1"
/locus_tag="P301_G10301"
/note="Similar to YGL223C Essential component of the
conserved oligomeric Golgi complex (Cog1p through Cog8p),
a cytosolic tethering complex that functions in protein
trafficking to mediate fusion of transport vesicles to
Golgi compartments"
/codon_start=1
/product="Cog1p"
/protein_id="EWG90818.1"
/translation="MDEVLPLFRDSHIPQIKDYQLELQNDLTKTNEAFQKNLLKNYNK
ILSLTDSVNDLSLNLKNVDQDFKSLCFNDEKFQLNKLAPLPYQTTTHISPPRDEEKVS
IPSQNILVISNWTISINNFCNRIVTSTTPSRIFDELLLNFHELSLIPVPSKFEALVKD
KCCRLQKFLVDSMKTLNLTLLQWVKLYNLLNTEFSSKWDDDLLSIFNESLFETLFNDN
VQALLISSANSKDHQYYSNQQYKDAIVVDFVNSSTFRDHLIRRTVKEINTHLDTLSTL
RAKLKEPETLHQLDIFHDNDTNLNDGTVSPLDDDALKQYIDTAVFYSKGLTNDTTLQI
YQTVQPTIEILQNLELYKCPQETLTDLRNKLITQLQEFKTQISSRLPSPLENSTSVVD
DFITSYNNHNLLQLVIDQITQLRQQ"
assembly_gap 79571..79586
/estimated_length=16
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(79611..80138)
/gene="Edc1"
/locus_tag="P301_G10311"
mRNA complement(79611..80138)
/gene="Edc1"
/locus_tag="P301_G10311"
/product="Edc1p"
CDS complement(79611..80138)
/gene="Edc1"
/locus_tag="P301_G10311"
/note="Similar to YGL222C 'RNA-binding protein, activates
mRNA decapping directly by binding to the mRNA substrate
and enhancing the activity of the decapping proteins Dcp1p
and Dcp2p; has a role in translation during heat stress'"
/codon_start=1
/product="Edc1p"
/protein_id="EWG90819.1"
/translation="MSTDTMYFNSSRLLPSAGRNKTNNLIKQKTRNNRARGNAAKNAN
NNNYITDIPPPQTLPNGQKPNFGHSSYKKPSFNQKKHSPPSSPSSTTTSGKKNRQNNK
ETPRQNNKDDTRLLSQNLKNLLLNQKQSPHGSQGIIPMGCNGSAKKLSHSYAGSTFAT
NGPREAKNLPKPSFL"
gene complement(80378..81244)
/gene="Nif3"
/locus_tag="P301_G10316"
mRNA complement(80378..81244)
/gene="Nif3"
/locus_tag="P301_G10316"
/product="Nif3p"
CDS complement(80378..81244)
/gene="Nif3"
/locus_tag="P301_G10316"
/note="Similar to YGL221C 'Protein of unknown function,
similar to Listeria monocytogenes major sigma factor (rpoD
gene product); the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Nif3p"
/protein_id="EWG90820.1"
/translation="MSRAITRAQLDKLVRSITKFYPQKYADKSWDNTGLLIDCSTAQV
TTADANAKTKVLLTVDLTKSVAQEAVDANCNVIVAYHPFIFPSWNRLSPHTNPQHETA
IKLIQYGISVYCPHTAVDAARGGVNDWLVRGLNNGENVAKSYALETVSGETDDLIGYG
RFVEFNKDISLEQIVKNVKRVLRVPYVQVASLAAPSAWNQLKIKKVAVCAGSGSGVFK
QLKEDVDLYYTGEMSHHEVLKWKEMGKTVIVCNHSNTERGFLQDVMKGLLQDEGHEVV
VSKMDCDPLTVA"
gene 81326..81688
/gene="Aim15"
/locus_tag="P301_G10321"
mRNA 81326..81688
/gene="Aim15"
/locus_tag="P301_G10321"
/product="Aim15p"
CDS 81326..81688
/gene="Aim15"
/locus_tag="P301_G10321"
/note="Similar to YGL220W 'Protein involved in negative
regulation of transcription of iron regulon; forms an iron
independent complex with Fra2p, Grx3p, and Grx4p; null
mutant fails to repress iron regulon and is sensitive to
nickel'"
/codon_start=1
/product="Aim15p"
/protein_id="EWG90821.1"
/translation="MTGERIEKVKINDEFAKSHFLTTQWRETKRQRHYKMPVTEQGLR
ERIESAIPQVYHIIVTDLSYGCGQSFDIVVVSDFFQGKSKLMRSRAVNKAVKEELQEI
HAFSCKCYTEEEWSKIVV"
gene complement(81830..83209)
/gene="Mdm34"
/locus_tag="P301_G10326"
mRNA complement(81830..83209)
/gene="Mdm34"
/locus_tag="P301_G10326"
/product="Mdm34p"
CDS complement(81830..83209)
/gene="Mdm34"
/locus_tag="P301_G10326"
/note="Similar to YGL219C Mitochondrial component of the
ERMES complex that links the ER to mitochondria and may
promote inter-organellar calcium and phospholipid exchange
as well as coordinating mitochondrial DNA replication and
growth"
/codon_start=1
/product="Mdm34p"
/protein_id="EWG90822.1"
/translation="MSFRFNEAVFGDNSFNERVREKLSTALNSPSKKKLDILKSGIKV
QKVDFPTIPQLEILDLDIITQPKSLAKGICKISCKDAMLRIQTVIESNLLLINEQDTP
SFTMPQLINNGSFTIPITMTFSSIELEAITNIFVKNPGIGISFNDVDLDFKFDCSVKI
LQSTIERRLKESMHVVFKDVLPSLIFNTSQNWFTNRGESTSTIPGKREHHHQQTTMSR
NVILDGSDFQELSPINMLRLSSIVSSRSTLSLHSTVMNSLSAIPGCLERQNLYRFISR
MPSLNNYYSSQSFPQPKSSTVSSKQLVKPFYCSHNLLPKTVLDSSQYDLATITKIQSR
LFDRSNSNDDNAKPRRRKIKCKKTRTPSNLQSQGEQAVDDSTAIETVTSTPVQTPIPE
LEEQSPPYLKTTVSIRDKYVIPEKISLNLDSKKDTSKKKPFYFIGLNSQEPSNNWKWG
MEDSPPPYH"
gene 83839..86226
/gene="Kip3"
/locus_tag="P301_G10341"
mRNA 83839..86226
/gene="Kip3"
/locus_tag="P301_G10341"
/product="Kip3p"
CDS 83839..86226
/gene="Kip3"
/locus_tag="P301_G10341"
/note="Similar to YGL216W Kinesin-related motor protein
involved in mitotic spindle positioning"
/codon_start=1
/product="Kip3p"
/protein_id="EWG90823.1"
/translation="MNVPETRQSSIVVAIRVRPFTSMEKTRLVNEASGAEANFPGLGD
SSLILPMSNNSDSDIDIDAEEGSTRSKRNSLLRRRVIRPEGIRKIVDCVDDRMLIFDP
ADRNPLNKVSDQVLNSMRARATKATASSINNSNATNKFSSQRRRHGGEIKFVFDKLFD
ETSSQARVYKETTSPLLDSVLDGFNSXXXXXXXGTPSQPGIIFLAMEELFNKITDLKD
EKDFEISLSYLEIYNERIRDLLKPETPSKRLVIREDTQNHIKVANLSYHHPNTVEDVM
DLVVQGNINRTTSPTEANEVSSRSHAVLQIHIMQTNKLVDLTSQHTFATLSIIDLAGS
ERAAATRNRGIRLHEGANINRSLLALGNCINALCLNDGSRSCHIPYRDSKLTRLLKFS
LGGNCKTVMIVCISPSSSHYDETLNTLKYANRAKEIKTKIIRNQQSLSRHVGSYLKMI
TEQKRQIEELREREEKMISLKLTKYKLNKEKIQLAINECVNRVQQTYAGVETYQVAKT
LKSLILCKRRFLQMVKLEVDNLILLFEREESIAAEMQPVISNCRMISGQLYNKIHELE
MKFDETDTLSSVIHQVHSIDLNKLREMEDWDETYDLVYLESCLNQISELQRNEILVNS
SIMTEKLMSDPGLNSRFKFLSKWLMNRTPNIESIIQDLVHIDEEFESFARTFIANPDS
NFTNTNINIINTTAADLAVPAETLQRQNFSKKKVKWTSPDLSPSPMIEPQPELEPELH
QDQDAIASEVDVSMQDTTFNEQGPSTPSAPTTAVPRRKMRSSLLTHQSLLATARK"
assembly_gap 84398..84415
/estimated_length=18
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 86904..88262
/gene="Clg1"
/locus_tag="P301_G10346"
mRNA 86904..88262
/gene="Clg1"
/locus_tag="P301_G10346"
/product="Clg1p"
CDS 86904..88262
/gene="Clg1"
/locus_tag="P301_G10346"
/note="Similar to YGL215W 'Cyclin-like protein that
interacts with Pho85p; has sequence similarity to G1
cyclins PCL1 and PCL2'"
/codon_start=1
/product="Clg1p"
/protein_id="EWG90824.1"
/translation="MANTFKYYPETMGNSSGYPISLPFPKGSATSAVNVARQLPKYLG
HVPSQSVHTQLPSMASLGYFNQPSSTYYAPPAPLQQHQQPPILPPPGLMYTSNNNSNV
IPPPVQMIRDGQQQPQQSNQVNGGVSENLDYDISIMSKFIMENAFVAFNANYSTDDQT
TDLFFKGISSVLNATRLPSATIFLAIDYLFKYINKLSNGIHSIGGNSINIIYQNTMIA
FILANKFNDDKTFTNSSWSQATGILINVINDFERQWLRIFNWELYDSAFLYFEFVKNF
EIFKQNQLKPAVAVPTLLSPIVNVGDTRNVNFNLKPTSTNNLLSPVSNYETPMLMPHN
MFSSPSYQSNSRSEFSSMNGYYNYYNYNQPRLNYYQQFPNIYSSPISETQFDYDFYNF
SSQQQQQQQKQHSLLPAAPQLPPPHVHNQSYGHHLGWKSMDDTINHSRFERSYFPYSA
VY"
assembly_gap 88555..88721
/estimated_length=167
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(88881..90074)
/gene="Ski8"
/locus_tag="P301_G10356"
mRNA complement(88881..90074)
/gene="Ski8"
/locus_tag="P301_G10356"
/product="Ski8p"
CDS complement(88881..90074)
/gene="Ski8"
/locus_tag="P301_G10356"
/note="Similar to YGL213C 'Ski complex component and
WD-repeat protein, mediates 3'-5' RNA degradation by the
cytoplasmic exosome; also required for meiotic
double-strand break recombination; null mutants have
superkiller phenotype'"
/codon_start=1
/product="Ski8p"
/protein_id="EWG90825.1"
/translation="MSKVFIATANAGKAHDADIFSVSACNSFTVSCSGDGYLKVWDNK
LLDNENPKDKSYSHFVHKSGLHHVDVLQTIERDAFELCLVATTSFSGDLLFYRITRED
ETKKVIFEKLDLLDSDMKKHSFWALKWGASNDRLLSHRLVATDVKGTTYIWKFHPFAD
ESNSLTLNWSPTLELQGTVESPMTPSQFATSVDISERGLIATGFNNGTVQISELSTLR
PLYNFESQHSMINNSNSIRSVKFSPQGSLLAIAHDSNSFGCITLYETEFGERIGSLSV
PTHSSQASLGEFAHSSWVMSLSFNDSGETLCSAGWDGKLRFWDVKTKERITTLNMHCD
DIEIEEDILAVDEHGDSLAEPGVFDVKFLKKGWRSGMGADLNESLCCVCLDRSIRWFR
EAGGK"
gene 90258..91208
/gene="Vam7"
/locus_tag="P301_G10361"
mRNA 90258..91208
/gene="Vam7"
/locus_tag="P301_G10361"
/product="Vam7p"
CDS 90258..91208
/gene="Vam7"
/locus_tag="P301_G10361"
/note="Similar to YGL212W 'Vacuolar SNARE protein that
functions with Vam3p in vacuolar protein trafficking; has
an N-terminal PX domain (phosphoinositide-binding module)
that binds PtdIns-3-P and mediates membrane binding;
SNAP-25 homolog'"
/codon_start=1
/product="Vam7p"
/protein_id="EWG90826.1"
/translation="MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSE
FWKLKTRLERDVGSTIPYDFPEKPGVLDRMWQRRYDDPEMIDERRIGLERFLNELYND
RFDSRWRDTKIAQDFLQLSKPNVSQEKSQQHLETADEVGWDEMIRDIKLDLDKESDGT
PSVRGALRARTKLHKSRERLEQDVQKKSLPSTEVTRRAALLRSLLKECDDIGTANIAQ
DRGRLLGVATSDNSSTMEVQGRTNNDLQQGQMQMVRDQEQELVALHRIIQAQRGLALE
MNEELQTQNELLTALEDDVDNTGRRLQIANKKARHFNNSA"
gene 91339..92418
/gene="Ncs6"
/locus_tag="P301_G10366"
mRNA 91339..92418
/gene="Ncs6"
/locus_tag="P301_G10366"
/product="Ncs6p"
CDS 91339..92418
/gene="Ncs6"
/locus_tag="P301_G10366"
/note="Similar to YGL211W 'Protein required for thiolation
of the uridine at the wobble position of Gln, Lys, and Glu
tRNAs; has a role in urmylation and in invasive and
pseudohyphal growth; inhibits replication of Brome mosaic
virus in S. cerevisiae'"
/codon_start=1
/product="Ncs6p"
/protein_id="EWG90827.1"
/translation="MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQ
CFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVL
LSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYC
GVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGS
PIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCI
IDIIQSGENLALKAKKSNARKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRA
QVAIENDTSADGAALKLRALEKLSF"
gene 92619..93287
/gene="Ypt32"
/locus_tag="P301_G10371"
mRNA 92619..93287
/gene="Ypt32"
/locus_tag="P301_G10371"
/product="Ypt32p"
CDS 92619..93287
/gene="Ypt32"
/locus_tag="P301_G10371"
/note="Similar to YGL210W 'Rab family GTPase, very similar
to Ypt31p; involved in the exocytic pathway; mediates
intra-Golgi traffic or the budding of post-Golgi vesicles
from the trans-Golgi'"
/codon_start=1
/product="Ypt32p"
/protein_id="EWG90828.1"
/translation="MSNEDYGYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKS
TIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYE
NCNHWLTELRENADDNVAVGLIGNKSDLAHLRAVPTDEAKNFAMENQMLFTETSALNS
DNVDKAFRELIVAIFQMVSKHQVDLSGSGTNNMGSNGAPKGPTISLTPAPKEDKKKKS
SNCC"
gene 94685..95833
/gene="Mig2"
/locus_tag="P301_G10386"
mRNA 94685..95833
/gene="Mig2"
/locus_tag="P301_G10386"
/product="Mig2p"
CDS 94685..95833
/gene="Mig2"
/locus_tag="P301_G10386"
/note="Similar to YGL209W 'Protein containing zinc
fingers, involved in repression, along with Mig1p, of SUC2
(invertase) expression by high levels of glucose; binds to
Mig1p-binding sites in SUC2 promoter'"
/codon_start=1
/product="Mig2p"
/protein_id="EWG90829.1"
/translation="MPKKQTNFPVDNENRPFRCDTCHRGFHRLEHKKRHLRTHTGEKP
HHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRLKKASVQKQEFLTVSGIPTIASGVMI
HQPIPQVLPANMAINVQAVNGGNIIHAPNAVHPMVIPIMAQPAPIHASAASFQPATSP
MPISTYTPVPSQSFTSFQSSIGSIQSNSDVSSIFSNMNVRVNTPRSVPNSPNDGYLHQ
QHIPQQYQHQTASPSVAKQQKTFAHSLASALSTLQKRTPVSAPSTTIESPSSPSDSSH
TSASSSAISLPFSNAPSQLAVAKELESVYLDSNRYTTKTRRERAKFEIPEEQEEDTNN
SSSSSNEEEHESLDHESSKSRKKLSGVKLPPVRNLLKQIDVFNGPKRV"
gene 96165..97412
/gene="Sip2"
/locus_tag="P301_G10391"
mRNA 96165..97412
/gene="Sip2"
/locus_tag="P301_G10391"
/product="Sip2p"
CDS 96165..97412
/gene="Sip2"
/locus_tag="P301_G10391"
/note="Similar to YGL208W 'One of three beta subunits of
the Snf1 serine/threonine protein kinase complex involved
in the response to glucose starvation; null mutants
exhibit accelerated aging; N-myristoylprotein localized to
the cytoplasm and the plasma membrane'"
/codon_start=1
/product="Sip2p"
/protein_id="EWG90830.1"
/translation="MGTTTSHPAQKKQTTKKCRAPIMSDVREKPSNAQGCEPQEMDAV
SKKVTELSLNKCSDSQDAGQPSREGSITKKKSTLLLRDEDEPTMPKLSVMETAVDTDS
GSSSTSDDEEGDIIAQTTEPKQDASPDDDRSGHSSPREEGQQQIRAKEASGGPSEIKS
SLMVPVEIRWQQGGSKVYVTGSFTKWRKMIGLIPDSDNNGSFHVKLRLLPGTHRFRFI
VDNELRVSDFLPTATDQMGNFVNYIEVRQPEKKPTNEKIRSKEAYSMRPPTSDRSSIA
LQIGKDPDDFGDGYTRFHEDLSPRPPLEYTTDIPAVFTDPSVMERYYYTLDRQQNNTD
TSWLTPPQLPPQLENVILNKYYATQDQFNENNSGALPIPNHVVLNHLVTSSIKHNTLC
VASIVRYKQKYVTQILYTPIESS"
gene 97799..100906
/gene="Spt16"
/locus_tag="P301_G10401"
mRNA 97799..100906
/gene="Spt16"
/locus_tag="P301_G10401"
/product="Spt16p"
CDS 97799..100906
/gene="Spt16"
/locus_tag="P301_G10401"
/note="Similar to YGL207W Subunit of the heterodimeric
FACT complex (Spt16p-Pob3p), which associates with
chromatin via interaction with Nhp6Ap and Nhp6Bp, and
reorganizes nucleosomes to facilitate access to DNA by RNA
and DNA polymerases"
/codon_start=1
/product="Spt16p"
/protein_id="EWG90831.1"
/translation="MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENP
YQKTTILHNWLLSYEFPATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLEL
WQRNNKEPEHNKKLFDDVIALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEF
NVIDISLGLSKVWEVKDVNEQAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLS
DKIENKIDDVKFLKQLSPDLSALCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARST
NDQLYGNGCILASCGIRYNNYCSNITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKP
GRTPKEVYESVIEYIEKTKPELVPNFTKNIGSLIGLEFRDSNFILNAKNDYRKIQRGD
CFNISFGFNNLKDSQSANNYALQLADTVQIPLDETEPPRFLTNYTKAKSQISFYFNNE
EEDNNKKKSSPATKVPSKPDRNSKILRTKLRGEARGGAEDAQKEQIRKENQKKLHEKL
EKNGLLRFSAADANGPDSEPRQYFKKYESYVRDSQLPTNIRDLRIHVDWKSQTIILPI
YGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPGSSGGISKKVEELPYEESADNQFVR
SITLRSKDGDRMSETFKQIADLKKEATKREQERKALADVVQQDKLIENKTGRTKRLDQ
IFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDILFSNIKNLIFQSCKGELIVVI
HIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQSRFRRYGDEDELEQEQEER
RKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNRSAVFCMPTTDCLVQLI
EPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHINTVPIESLDFLKQWL
TDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDEASDESEEEVSEY
EASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDELEKKAARADRG
ANFRD"
gene complement(101373..106334)
/gene="Chc1"
/locus_tag="P301_G10406"
mRNA complement(101373..106334)
/gene="Chc1"
/locus_tag="P301_G10406"
/product="Chc1p"
CDS complement(101373..106334)
/gene="Chc1"
/locus_tag="P301_G10406"
/note="Similar to YGL206C 'Clathrin heavy chain, subunit
of the major coat protein involved in intracellular
protein transport and endocytosis; two heavy chains form
the clathrin triskelion structural component; the light
chain (CLC1) is thought to regulate function'"
/codon_start=1
/product="Chc1p"
/protein_id="EWG90832.1"
/translation="MSDLPIEFTELVDLTSLGISPQFLDFRSTTFESDHFVTVRETKD
GTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSF
TLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFV
ANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQ
VFVTGNRNATTGAGELRIIEIDHDASLPSQYQKKTTDIFFPPDATNDFPIAVQVSEKY
GIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVE
ISTSQIVPYILNKLSNVALALTVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAAS
STSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQ
LFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPY
CQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQ
QGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSE
KAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQ
ANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEA
AAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLY
KSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPIDELTTEVE
KRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHY
CEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIH
RRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVAL
QGLLLLSAIKYEPTKVSRYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKAL
KVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENV
IDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAELNKIHEIENLLAGSNVANL
DHVGDKLFENKGYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLV
NDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHM
GMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWD
NAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRL
DIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYD
KFDQLGLASRLESHKLIFFKKIAALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQD
PKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKK
EQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF"
gene 106989..109235
/gene="Pox1"
/locus_tag="P301_G10411"
mRNA 106989..109235
/gene="Pox1"
/locus_tag="P301_G10411"
/product="Pox1p"
CDS 106989..109235
/gene="Pox1"
/locus_tag="P301_G10411"
/note="Similar to YGL205W 'Fatty-acyl coenzyme A oxidase,
involved in the fatty acid beta-oxidation pathway;
localized to the peroxisomal matrix'"
/codon_start=1
/product="Pox1p"
/protein_id="EWG90833.1"
/translation="MTRRTTINPDSVVLNPQKFIQKERADSKIKVDQVNTFLESSPER
RTLTHALIDQIVNDPILKTDTDYYDATKLQEREITAKKIARLASYMEHDIKTVRKHFR
NTDLMKELQANDPDKASPLTNKDLFIFDKRLSLVANIDPQLGTRVGVHLGLFGNCIKG
NGTDEQIRYWLQERGATLMKGIYGCFAMTELGHGSNVAQLQTRAVYDKQNDTFIIDTP
DLTATKWWIGGAAHSATHAAVYARLIVEGKDYGVKTFVVPLRDPSTFQLLAGVSIGDI
GAKMGRDGIDNGWIQFRNVVIPREFMLSRFTKVVRSPDGSVTVKTEPQLDQISGYSAL
LSGRVNMVMDSFRFGSKFATIAVRYAVGRQQFAPRKGLSETQLIDYPLHQYRVLPQLC
VPYLVSPVAFKLMDNYYSTLDELYNASSSADKAALVTVSKKLKNLFIDSASLKATNTW
LIATLIDELRQTCGGHGYSQYNGFGKGYDDWVVQCTWEGDNNVLSLTSAKSILKKFID
SATKGRFDNTLDVDSFSYLKPQYIGSVVSGETKSSLKELGDYTEIWSITLIKLLAHIG
TLVEKSRSIDSVSKLLVLVSKFHALRCMLKTYYDKLNSRDSHISDEITKESMWNVYKL
FSLYFIDKHSGEFQQFKIFTPDQISKVVQPQLLALLPIVRKDCIGLTDSFELPDAMLN
SPIGYFDGDIYHNYFNEVCRNNPVEADGAGKPSYHALLSSMLGRGFEFDQKLGGAANA
EILSKINK"
assembly_gap 109454..110284
/estimated_length=831
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(111201..113414)
/gene="Kex1"
/locus_tag="P301_G10426"
mRNA complement(111201..113414)
/gene="Kex1"
/locus_tag="P301_G10426"
/product="Kex1p"
CDS complement(111201..113414)
/gene="Kex1"
/locus_tag="P301_G10426"
/note="Similar to YGL203C 'Protease involved in the
processing of killer toxin and alpha factor precursor;
cleaves Lys and Arg residues from the C-terminus of
peptides and proteins'"
/codon_start=1
/product="Kex1p"
/protein_id="EWG90834.1"
/translation="MFYNRWLGTWLAMSALIRISVSLPSSEEYKVAYELLPGLSEVPD
PSNIPQMHAGHIPLRSEDADEQDSSDLEYFFWKFTNNDSNGNVDRPLIIWLNGGPGCS
SMDGALVESGPFRVNSDGKLYLNEGSWISKGDLLFIDQPTGTGFSVEQNKDEGKIDKN
KFDEDLEDVTKHFMDFLENYFKIFPEDLTRKIILSGESYAGQYIPFFANAILNHNKFS
KIDGDTYDLKALLIGNGWIDPNTQSLSYLPFAMEKKLIDESNPNFKHLTNAHENCQNL
INSASTDEAAHFSYQECENILNLLLSYTRESSQKGTADCLNMYNFNLKDSYPSCGMNW
PKDVSFVSKFFSTPGVIDSLHLDSDKIDHWKECTNSVGTKLSNPISKPSIHLLPGLLE
SGIEIVLFNGDKDLICNNKGVLDTIDNLKWGGIKGFSDDAVSFDWIHKSKSTDDSEEF
SGYVKYDRNLTFVSVYNASHMVPFDKSLVSRGIVDIYSNDVMIIDNNGKNVMITTDDD
SDQDATTESGDKPKENLEEEEQEAQNEEGKEKEGNKDKDGDDDNDNDDDDEDDHNSEG
DDDDDDDDDDDDDDDDDEDDNNEKQSNQGLEDSRHKSSEYEQEEEEVEEFAEEISMYK
HKAVVVTIVTFLIVVLGVYAYDRRVRRKARHTILVDPNNRQHDSPNKTVSWADDLESG
LGAEDDLEQDEQLEGGAPISSTSNKAGSKLKTKKKKKYTSLPNTEIDESFEMTDF"
assembly_gap 114259..114358
/estimated_length=100
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 114828..116330
/gene="Aro8"
/locus_tag="P301_G10436"
mRNA 114828..116330
/gene="Aro8"
/locus_tag="P301_G10436"
/product="Aro8p"
CDS 114828..116330
/gene="Aro8"
/locus_tag="P301_G10436"
/note="Similar to YGL202W Aromatic aminotransferase I,
expression is regulated by general control of amino acid
biosynthesis"
/codon_start=1
/product="Aro8p"
/protein_id="EWG90835.1"
/translation="MTLPESRDFSYLFSDETNARKPSPLKTCIHLFQDPNIIFLGGGL
PLKDYFPWDNLSVDSPKPPFPQGIGAPIDEQNCIKYTVNKDYADKSANPSNDIPLSRA
LQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVIL
VEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTG
QNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQD
HDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTI
QAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAFVIN
PPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGET
EPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRLGDTLYEEFGISK"
gene complement(116623..119667)
/gene="Mcm6"
/locus_tag="P301_G10441"
mRNA complement(116623..119667)
/gene="Mcm6"
/locus_tag="P301_G10441"
/product="Mcm6p"
CDS complement(116623..119667)
/gene="Mcm6"
/locus_tag="P301_G10441"
/note="Similar to YGL201C 'Protein involved in DNA
replication; component of the Mcm2-7 hexameric complex
that binds chromatin as a part of the pre-replicative
complex; forms a subcomplex with Mcm4p and Mcm7p'"
/codon_start=1
/product="Mcm6p"
/protein_id="EWG90836.1"
/translation="MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLH
FPSSSQPHVSNSQTGPFVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKK
VDDVTGEKVREAFEQFLEDFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSM
RENGALAMAISEQYYRFLPFLQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADDDEQ
QDDDMNGSSLPRDSGSSAAPGNGTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRI
RDIRSEKIGSLLSISGTVTRTSEVRPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCP
NPSCENRAFWTLNVTRSRFLDWQKVRIQENANEIPTGSMPRTLDVILRGDSVERAKPG
DRCKFTGVEIVVPDVTQLGLPGVKPSSTLDTRGISKTTEGLNSGVTGLRSLGVRDLTY
KISFLACHVISIGSNIGASSPDANSNNRETELQMAANLQANNVYQDNERDQEVFLNSL
SSDEINELKEMVKDEHIYDKLVRSIAPAVFGHEAVKKGILLQMLGGVHKSTVEGIKLR
GDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSGKASSAAGLTAAVVRDEEGGDYTIE
AGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQTISIAKAGIHATLNARTSILAAA
NPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCNEKIDTELASHIVDLHMKRDE
AIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKELRKDDAQGFSRSSYRITVR
QLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDVDDVEMDEEFDNIESQS
HAASGNNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKKKTTVTYDKYVSMMNMIV
RKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRLAFKVIKRLVKDRILM
EIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS"
gene 121029..121101
/locus_tag="P301_G10451"
tRNA 121029..121101
/locus_tag="P301_G10451"
/product="tRNA-Lys"
/note="TK(CUU)G1"
gene complement(121453..122064)
/gene="Emp24"
/locus_tag="P301_G10456"
mRNA complement(121453..122064)
/gene="Emp24"
/locus_tag="P301_G10456"
/product="Emp24p"
CDS complement(121453..122064)
/gene="Emp24"
/locus_tag="P301_G10456"
/note="Similar to YGL200C Integral membrane component of
endoplasmic reticulum-derived COPII-coated vesicles, which
function in ER to Golgi transport"
/codon_start=1
/product="Emp24p"
/protein_id="EWG90837.1"
/translation="MASFATKFVIACFLFFSASAHNVLLPAYGRRCFFEDLSKGDELS
ISFQFGDRNPQSSSQLTGDFIIYGPERHEVLKTVRDTSHGEITLSAPYKGHFQYCFLN
ENTGIETKDVTFNIHGVVYVDLDDPNTNTLDSAVRKLSKLTREVKDEQSYIVIRERTH
RNTAESTNDRVKWWSIFQLGVVIANSLFQIYYLRRFFEVTSLV"
gene 122350..123057
/gene="Yip4"
/locus_tag="P301_G10466"
mRNA 122350..123057
/gene="Yip4"
/locus_tag="P301_G10466"
/product="Yip4p"
CDS 122350..123057
/gene="Yip4"
/locus_tag="P301_G10466"
/note="Similar to YGL198W 'Protein that interacts with Rab
GTPases, localized to late Golgi vesicles; computational
analysis of large-scale protein-protein interaction data
suggests a possible role in vesicle-mediated transport'"
/codon_start=1
/product="Yip4p"
/protein_id="EWG90838.1"
/translation="MSYGREDTTIEPDFIEPDAPLAASGGVADNIGGTMQNSGSRGTL
DETVLQTLKRDVVEINSRLKQVVYPHFPSFFSPSDDGIGAADNDISANCDLWAPLAFI
ILYSLFVSHARSLFSSLFVSSWFILLVMALHLRLTKPHQRVSLISYISISGYCLFPQV
LNALVSQILLPLAYHIGKQNRWIVRVLSLVKLVVMALCLMWSVAAVSWVTKSKTIIEI
YPLALCLFGMAWLSTIL"
gene 123456..127921
/gene="Mds3"
/locus_tag="P301_G10471"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 125870..125942
/estimated_length=73
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 128643..129929
/gene="Dsd1"
/locus_tag="P301_G10476"
mRNA 128643..129929
/gene="Dsd1"
/locus_tag="P301_G10476"
/product="Dsd1p"
CDS 128643..129929
/gene="Dsd1"
/locus_tag="P301_G10476"
/note="Similar to YGL196W 'D-serine dehydratase (aka
D-serine ammonia-lyase); converts D-serine to pyruvate and
ammonia by a reaction dependent on pyridoxal 5'-phosphate
and zinc; may play a role in D-serine detoxification;
L-serine is not a substrate'"
/codon_start=1
/product="Dsd1p"
/protein_id="EWG90839.1"
/translation="MSDVLSQYKGCSVRDLPTPNFVINEEKFDKNCTTMLNNVEKLSQ
ECGVPIKFRAHVKTHKTAKGTLKQLGHGLPLAKRTTRAILVSTLKEAEELLNYQDRQC
SDYIDDITYSLPCCVPEFIPLLSNLSRRVNNFQVFVDNIEHLENLKNFGRPASGKKWS
VFIKVDMGTKRAGLAFDSPEFLSLLKKLTSSEIKEVIEPYGFYAHAGHSYSSTSINDT
QNLLMEEVKAVNSAAKVLCSVDPQFDPSKLTLSVGATPTSNSLKLDNKSTLVKFVTTQ
LVSTLEIHCGNYCMYDLQQVATGCVQDHELSGFVLGTVLSSYPSRGELLSNTGVMCLT
REASSIKGFGICADLEHVLKSESFSREWYVARVSQEHGILRPIRNWNETTALKLGSKI
AVLPQHACITMGQFPYYFVVNSQGIVNDVWLPFQKW"
gene 130285..138303
/gene="Gcn1"
/locus_tag="P301_G10481"
mRNA 130285..138303
/gene="Gcn1"
/locus_tag="P301_G10481"
/product="Gcn1p"
CDS 130285..138303
/gene="Gcn1"
/locus_tag="P301_G10481"
/note="Similar to YGL195W 'Positive regulator of the Gcn2p
kinase activity, forms a complex with Gcn20p; proposed to
stimulate Gcn2p activation by an uncharged tRNA'"
/codon_start=1
/product="Gcn1p"
/protein_id="EWG90840.1"
/translation="MTAILNWEDISPVLEKGTRESHVSKRVPFLQDISQLVRQETLEK
PQLSEIAFVLLNTFPIYEDNRSKSLVTSVLLDILNLEPCLLENFIRFISDVVISNPAT
KAVADYLNLLDWINSFLIFVSHNSNLFEEYIPKLLVVHSYATFGVETILDNQEEGKKS
QDKQNQHRKRIRYCIFQATVKAFLKCLKDNDDSISFMKISIKTVLESYSKLKITSVGV
VMIMGALTQAALQLLSRQPALHSVLKENSAEKYCEYLGKEVFLGKNPPSSFCLEIGLK
PFLKEFVSQELFIKFFIPNIEKAVLRSPEVGFSILSELYAGVSSEKVNLLNAFTSSKL
INQYFSSFKSSKEVVRSVSLQSMIILLRKISNTDTTLEDLTKLIDEIFKNIKSNLNAD
YKSLISKILIEIPLTHYEVSEKTCKGLSPYICKEGNEAALTLMLNAFFVHYFSLGKPI
EDLDKIISAGFADKKPALKKCWFAAFLNNSNAASEEVILNFIDGCLEFVKDSIIHYQT
HGHACILASIEFTNKILALDNTELNDRVMQLIETLPENSSIGDAILTAALSTELSIEN
RIHAVNLLQELFYKKPEFIGFSVIDAIERRMRVQELIPQQNTSFKYVTSVLLAITSEL
PDKEASIKVLINALVIAQWNIFNIKNGWAGLVLRARLDPAEVVKEHASVIMEKILEIT
GSCEWIDTIYGACGLQAAAYAAFIQPTEFTPILCKTIEADLTADDFSRLSEEDFEIFA
GEEGVLVVDVLEESMNKKLSNKNSKEYETLMWEQKIRKEQAKKNVKKLSKEEQELVNE
QLAKESAVRSHVSEISTRLKRGIRLVSELSKAACLVQNGIAIWFPLAVTKLLYLCSEP
NISKLTEDVNIVFLQLSQNVSERLGNIRLFLGLATLRVHNANGISQDYLQEPLVELLT
RVLFRIKFVSNQAAIDPISLTYILPLLINVLEKGKAIALKNADKPVVKAEFVEEDEEE
EHLLLAMEIISVHAEAFEDPSIPRISIVEVLLSLLSLPSKAKIAKDCFNALCQSISVA
PNQEDLDMILSNLLSPNQFVRSTILETLDNEFELEPFMKYSPEVFICRFDSDPSNREI
ADFIWEFNKFVVNDELLKSLFPLFNQDDSGLRLFAANAYAFGAVSLFTSEENSSKDYL
NDLLNFYKERAKPLEPILDQFGLVLVSASEQKDPWQGRSTVAITLKIMAKAFSAEDDT
VVNIIKFLVDDGGLVDREPIVRQEMKEAGVELITLHGSQNSKDLIPIFEEALSSSTDS
ALKENVIILYGTLARHLQQSDARIHTIIERLLSTLDTPSADIQQAVSACIAPLVFQFK
QKVGDYLGILMEKLLNPTVASSMRKGAAWGIAGLVKGYGISALSEFDIIRNLIEAAED
KKEPKRRESVGFCFQYLSESLGKFFEPYVIEILPNILKNLGDAVPEVRDATARATKAI
MAHTTGYGVKKLIPVAVSNLDEIAWRTKRGSVQLLGNMAYLDPTQLSASLSTIVPEIV
GVLNDSHKEVRKAADESLKRFGEVIRNPEIQKLVPVLLQAIGDPTKYTEEALDSLIQT
QFVHYIDGPSLALIIHIIHRGMHDRSANIKRKACKIVGNMAILVDTKDLIPYLQQLID
EVEIAMVDPVPNTRATAARALGALVERLGEEQFPDLIPRLLDTLSDESKSGDRLGSAQ
ALAEVISGLGLTKLDEMLPTILAGVTNFRAYIREGFMPLLLFLPVCFGSQFAPYINQI
IQPILSGLADNDENIRDTALKAGKLIVKNYATKAVDLLLPELERGMFDENDRIRLSSV
QLTGELLFQVTGISSRNEFSEEDGDHNGEFSGKLVDVLGQDRRDRILAALFVCRNDTS
GIVRATTVDIWKALVPNTPRAVKEILPTLTGMIVTHLASSSNVLRNIAAQTLGDLVRR
VGGNALSQLLPSLEESLIETSNSDSRQGVCIALYELIESASTETISQFQSTIVNIIRT
ALIDESATVREAAALSFDVFQDVVGKTAVDEVLPYLLHMLESSDNSDFALLGLQEIMS
KKSDVIFPILIPTLLAPPIDAFRASALGSLAEVAGSALYKRLSIIINALVDAIIGTSE
DESTKGALELALDRVFLSVNDDEGLHPLLQQIMSLLKSDNIEKRIAVLERLPNFFDKT
VLDFDVYIPNFVSHAILSLDDEDQRVVNGNFNALSTLLKKVDKPTLEKLVKPAKQSLA
LTGRQGQDVAAFKLPRGPNCILPIFLHGLMYGSNDEREESALAIADVVSKTPAANLKP
FVSVITGPLIRVVGERFSSDIKAAILFALNVLFIKIPMFLRPFIPQLQRTFVKSLSDA
TNETLRLRAAKALGALIEHQPRVDPLVIELVTGAKQATDEGVKTAMLKALLEVIMKAG
SKLNENSKTNIVNLVEEEMLGSNDKLAVAYAKLIGSLSEILSNDEAHKILQDKVLNAD
LDGETGKFAILTLNSFLKDAPTHIFNTGLIDEFVSYILNAIRSPDVYFGENGTIAAGK
LLLLEGEKRSPFVKKDAAEPFKIGDENINLLINELSKAVLQPASNSTDVRRLALVVIR
TLARFKFDECIKQFFDVVGPSVFSCLRDPVIPIKLAAEKAYLALFKLVEEDDMHTFNE
WFAEISDRGSSIETVTGTTIQLRSVGDYTKRVGKRLANVERERIAAGGDAETMFSDRF
EDEREIWAVGGVELTTDV"
gene complement(138479..138721)
/locus_tag="P301_G10486"
mRNA complement(138479..138721)
/locus_tag="P301_G10486"
/product="hypothetical protein"
CDS complement(138479..138721)
/locus_tag="P301_G10486"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90841.1"
/translation="MSNKEITCIKPFKIIALTLLIVLIINLSYKLFLRRYLKSSVIWC
LGIANTDRNDIMWWQVSPLLERWVWQLVDNYESGYE"
assembly_gap 139051..139220
/estimated_length=170
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<139221..140487)
/gene="Hos2"
/locus_tag="P301_G10491"
mRNA complement(<139221..140487)
/gene="Hos2"
/locus_tag="P301_G10491"
/product="Hos2p"
CDS complement(<139221..140487)
/gene="Hos2"
/locus_tag="P301_G10491"
/note="Similar to YGL194C 'Histone deacetylase required
for gene activation via specific deacetylation of lysines
in H3 and H4 histone tails; subunit of the Set3 complex, a
meiotic-specific repressor of sporulation specific genes
that contains deacetylase activity'"
/codon_start=1
/product="Hos2p"
/protein_id="EWG91218.1"
/translation="MPGTFSYDVKTKENEPLFEFNSAYSPRVSYHFNSKVSHYHYGVK
HPMKPFRLMLTDHLVSSYGLHKIMDLYETRSATRDELLQFHSEDYVNFLSKVSPENAN
KLPRGTLENFNIGDDCPIFQNLYDYTTLYTGASLDATRKLINNQSDIAINWSGGLHHA
KKNSPSGFCYVNDIVLSILNLLRYHPRILYIDIDLHHGDGVQEAFYTTDRVFTLSLHK
YNGEFFPGTGDLTEIGCDKGKHFALNVPLEDGIDDDSYINLFKSIVDPLIMTFKPTLI
VQQCGADSLGHDRLGCFNLNIKAHGECVKFVKSFGLPMLVVGGGGYTPRNVSRLWTYE
TGILNDVLLPEDIPEDIPFRDSFGPDYSLYPVLDDLYENKNSKKLLEDIRIRCLENIR
YLQGAPSVRMDAECIPTQDISALTEEEDKI"
gene complement(140744..140986)
/locus_tag="P301_G10496"
mRNA complement(140744..140986)
/locus_tag="P301_G10496"
/product="hypothetical protein"
CDS complement(140744..140986)
/locus_tag="P301_G10496"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90842.1"
/translation="MNSSLNANSYFFRKPPMLTYMVRFLYCYPSPFPIAPAVTDLPEC
RGDLSLSLFITSFTSTKESNTVRQKPPKDTYSRQFM"
gene 141005..142807
/gene="Ime4"
/locus_tag="P301_G10501"
mRNA 141005..142807
/gene="Ime4"
/locus_tag="P301_G10501"
/product="Ime4p"
CDS 141005..142807
/gene="Ime4"
/locus_tag="P301_G10501"
/note="Similar to YGL192W 'Probable mRNA N6-adenosine
methyltransferase required for entry into meiosis;
transcribed in diploid cells; haploids repress IME4
transcription via production of antisense IME4
transcripts; antisense transcription is repressed in
diploids'"
/codon_start=1
/product="Ime4p"
/protein_id="EWG90843.1"
/translation="MINDKLVHFLIQNYDDILRAPLSGQLKDVYSLYISGGYDDEMQK
LRNGKDDVLQFEQFWNDLQDIIFATPQSIQFDQNLLVADRPEKIVYLDVFSLKILYNK
FHAFYYTLKSSSSSCEEKVSSSTTKPGADSEKDQLLGRLLGVLNWDVNVSNQGLPREQ
LSNRLQNLLREKPSSFQLAKERAKYTTEVIEYIPICSDYSHASLLSTSVYIVNNKIVS
LQWSKISACQENHPGLIECIQSKIHFIPNIKPQTDISLGDCSYLDTCHKLNTCRYIHY
LQYIPSCLQERADRETASENKRIRSNVSIPFYTLGNCSAHCIKKALPAQWIRCDVRKF
DFRVLGKFSVVIADPAWNIHMNLPYGTCNDIELLGLPLHELQDEGIIFLWVTGRAIEL
GKESLNNWGYNVINEVSWIKTNQLGRTIVTGRTGHWLNHSKEHLLVGLKGNPKWINKH
IDVDLIVSMTRETSRKPDELYGIAERLAGTHARKLEIFGRDHNTRPGWFTIGNQLTGN
CIYEMDVERKYQEFMKSKTGTSHTGTKKIDKKQPSKLQQQHQQQYWNNMDMRSGKYYA
EAKQNPMNQKHTPFESKQQQKQQFQTLNNLYFAQ"
gene 143567..143956
/gene="Cox13"
/locus_tag="P301_G10506"
mRNA 143567..143956
/gene="Cox13"
/locus_tag="P301_G10506"
/product="Cox13p"
CDS 143567..143956
/gene="Cox13"
/locus_tag="P301_G10506"
/note="Similar to YGL191W 'Subunit VIa of cytochrome c
oxidase, which is the terminal member of the mitochondrial
inner membrane electron transport chain; not essential for
cytochrome c oxidase activity but may modulate activity in
response to ATP'"
/codon_start=1
/product="Cox13p"
/protein_id="EWG90844.1"
/translation="MFRQCAKRYASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHA
KDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIR
SKPFFWGDGDKTLFWNPVVNRHIEHDD"
assembly_gap 144032..144371
/estimated_length=340
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(144571..146151)
/gene="Cdc55"
/locus_tag="P301_G10511"
mRNA complement(144571..146151)
/gene="Cdc55"
/locus_tag="P301_G10511"
/product="Cdc55p"
CDS complement(144571..146151)
/gene="Cdc55"
/locus_tag="P301_G10511"
/note="Similar to YGL190C 'Non-essential regulatory
subunit B of protein phosphatase 2A (PP2A), which has
multiple roles in mitosis and protein biosynthesis;
involved in regulation of mitotic exit; found in the
nucleus of most cells, also at bud neck and bud tip'"
/codon_start=1
/product="Cdc55p"
/protein_id="EWG90845.1"
/translation="MAQNNFDFKFSQCFGDKADIVVTEADLITAVEFDYTGNYLATGD
KGGRVVLFERSNSRHCEYKFLTEFQSHDAEFDYLKSLEIEEKINEIKWLRPTQRSHFL
LSTNDKTIKLWKVYEKNIKLVSQNNLTEGVTFAKKGKPDNHNSRGGSARAVLSLQSLK
LPQLSQHDKIIAATPKRIYSNAHTYHINSISLNSDQETFLSADDLRINLWNLDIPDQS
FNIVDIKPTNMEELTEVITSAEFHPQECNLFMYSSSKGTIKLCDMRQNSLCDNKTKTF
EEYLDPINHNFFTEITSSISDIKFSPNGRYIASRDYLTVKIWDVNMDNKPLKTINIHE
QLKERLSDTYENDAIFDKFEVNFSGDSSSVMTGSYNNNFMIYPNVVTSGDNDNGIVKT
FDEHNAPNSNSNKNIHNSIQNKDSSSSGNSHKRRSNGRNTGMVGSSNSSRSSIAGGEG
ANPEDSGTEMNEIVLQADKTAFRNKRYGSLAQRSARNKDWGDDIDFKKNILHFSWHPR
ENSIAVAATNNLFIFSAL"
gene complement(146988..147347)
/gene="Rps26a"
/locus_tag="P301_G10516"
mRNA complement(146988..147347)
/gene="Rps26a"
/locus_tag="P301_G10516"
/product="Rps26ap"
CDS complement(146988..147347)
/gene="Rps26a"
/locus_tag="P301_G10516"
/note="Similar to YGL189C 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps26Bp and
has similarity to rat S26 ribosomal protein'"
/codon_start=1
/product="Rps26ap"
/protein_id="EWG90846.1"
/translation="MPKKRASNGRNKKGRGHVKPVRCVNCSKSIPKDKAIKRMAIRNI
VEAAAVRDLSEASVYPEYALPKTYNKLHYCVSCAIHARIVRVRSREDRKNRAPPQRPR
FNRENKVSPADAAKKAL"
gene complement(147583..147723)
/locus_tag="P301_G10521"
mRNA complement(147583..147723)
/locus_tag="P301_G10521"
/product="hypothetical protein"
CDS complement(147583..147723)
/locus_tag="P301_G10521"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90847.1"
/translation="MLTTQKCESREGKNDEIFELGESNSDKILLKHGKCNLFSERKPV
NH"
gene complement(148461..148928)
/gene="Cox4"
/locus_tag="P301_G10536"
mRNA complement(148461..148928)
/gene="Cox4"
/locus_tag="P301_G10536"
/product="Cox4p"
CDS complement(148461..148928)
/gene="Cox4"
/locus_tag="P301_G10536"
/note="Similar to YGL187C 'Subunit IV of cytochrome c
oxidase, the terminal member of the mitochondrial inner
membrane electron transport chain; precursor N-terminal 25
residues are cleaved during mitochondrial import;
phosphorylated; spermidine enhances translation'"
/codon_start=1
/product="Cox4p"
/protein_id="EWG90848.1"
/translation="MLSLRQSIRFFKPATRTLCSSRYLLQQKPVVKTAQNLAEVNGPE
TLIGPGAKEGTVPTDLDQETGLARLELLGKLEGIDVFDTKPLDSSRKGTMKDPIIIES
YDDYRYVGCTGSPAGSHTIMWLKPTVNEVARCWECGSVYKLNPVGVPNDDHHH"
gene complement(149794..151533)
/gene="Tpn1"
/locus_tag="P301_G10541"
mRNA complement(149794..151533)
/gene="Tpn1"
/locus_tag="P301_G10541"
/product="Tpn1p"
CDS complement(149794..151533)
/gene="Tpn1"
/locus_tag="P301_G10541"
/note="Similar to YGL186C 'Plasma membrane pyridoxine
(vitamin B6) transporter; member of the purine-cytosine
permease subfamily within the major facilitator
superfamily; proton symporter with similarity to Fcy21p,
Fcy2p, and Fcy22p'"
/codon_start=1
/product="Tpn1p"
/protein_id="EWG90849.1"
/translation="MNRDNMDTTKRKEDHTKHTTDVIEFYEEGTAASSLNIATEKANS
SPSILRRIINRAAWLSKKVDAMGVESTGIQRISPYERGTSKKQFLHVAGLWLSATGGL
SSMSSFLLGPLLFGLSFRESVASSLISVTIGCLIAAYCSIMGPQSGCRQMVTARYLFG
WWFVKLVALASIIGVMGWSVVNSVVGGEMLAAISNDKVPLWVGIVIVTVCSFLVAIFG
IKQVIKVETYLSVPVLTAFLLLYISSSDKYSFVNAYVSKGNLDSSTRKGNWMSFFSLC
YSITATWGSITADYYILFPEDTPYIQIFCLTFFGTFLPTCFVGILGLLLASVAMSYKP
WSVEYDSHGMGGLLWAGFQRWNGFGKFCVVVLVFSLVSNNIINTYSAAFSIQLSSVFC
AKIPRWFWSIVCTIICLVCALIGRNHFSTILGNFLPMIGYWISMYFILLFEENLVFRR
FFLHLYTKEFPTVTGEINGPELVGSSKEVEKDAVTNIHLLKRKHKVTKHRYNWDKWED
YEVLTHGYAATFAFIVGVAGVVVGMAQAYWIGPIAAKFGEYGGDVAMWLSMAFSGVVY
PPCRYLELRKFGR"
gene complement(151924..153063)
/locus_tag="P301_G10546"
mRNA complement(151924..153063)
/locus_tag="P301_G10546"
/product="hypothetical protein"
CDS complement(151924..153063)
/locus_tag="P301_G10546"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90850.1"
/translation="MCDSPATTGKPTILFIADPCETSATLNSKAFKEKFRILRYQLDT
KEAFLNFLERHEQDKICAIYAGFPAFKKIGGMTRSIIEHKSFPRKNLKCIVLCSRGYD
GWDLDTLRKHEIRLYNYQDDENEKLIDDLKLHQVGNDVADCALWHILEGFRKFSYYQK
LSRETGNTLTARAKAAEKSGFAFGHELGNMFAESPRGKKCLILGLGSIGKQVAYKLQY
GLGIEIHYCKRSEDCTMSQNESWKFHLLDETIYAKLYQFHAIVVTLPGTPQTEHLINR
KFLEHCNPGLILVNLGRGKILDLRAVSDALVTGRINHLGLDVFNKEPEIDEKIRSSDR
LTSITPHLGSATKDVFEQSCELALTRILRVVSGEAASDEHFSRVV"
gene complement(153370..154767)
/gene="Str3"
/locus_tag="P301_G10551"
mRNA complement(153370..154767)
/gene="Str3"
/locus_tag="P301_G10551"
/product="Str3p"
CDS complement(153370..154767)
/gene="Str3"
/locus_tag="P301_G10551"
/note="Similar to YGL184C 'Peroxisomal cystathionine
beta-lyase, converts cystathionine into homocysteine; may
be redox regulated by Gto1p'"
/codon_start=1
/product="Str3p"
/protein_id="EWG90851.1"
/translation="MPIKRLDTVVVNTGSQNDQHSASVPPVYLSTTFKVDLNNEDAQN
YDYSRSGNPTRSVLQHQIGKLYRVPQENVLAVSSGMTALDVILRGLVLLNGTDNHTPT
IIAGDDLYGGTQRLLNFFKQQSHAVSVHVDTSDFEKFKTVFQSLDKVDCVLLESPTNP
LCKVVDIPRILRFVKCISPDTTVVVDNTMMSGLNCNPLQLNPGCDVVYESATKYLNGH
HDLMGGVIISKTPEIASKLYFVINSTGAGLSPMDSWLLVRGLKTLGVRLYQQQRNAMI
LAHWLENSCGFKPTRTNKATKTRFVGLRSNPDFKLHKSFNNGPGAVLSFETGSFEHSK
RLVSSKKLSIWAVTVSFGCVNSLLSMPCKMSHASIDPELRKERDFPEDLVRLCCGIEN
IVDLKKDLLAAMVDADIIEVRENGKYLFNKLNKNLAVNTTIDDLHKPLSIYEEFYNQD
LIRKDSELNIKSSKL"
gene complement(155210..156033)
/gene="Mnd1"
/locus_tag="P301_G10556"
mRNA complement(join(155210..155947,156031..156033))
/gene="Mnd1"
/locus_tag="P301_G10556"
/product="Mnd1p"
CDS complement(join(155210..155947,156031..156033))
/gene="Mnd1"
/locus_tag="P301_G10556"
/note="Similar to YGL183C 'Protein required for
recombination and meiotic nuclear division; forms a
complex with Hop2p, which is involved in chromosome
pairing and repair of meiotic double-strand breaks'"
/codon_start=1
/product="Mnd1p"
/protein_id="EWG90852.1"
/translation="MGPKRQTVSLQEKKNRILNFFQETYTFYNIKELEKSIPKKCGIS
PMIVKDLVQQMIDEDGVISVEKCGNINIYWCFKNQTLQKLYDSSELIKKKIQEVKCDI
ATYKQELDKTLATGRRKKFTVGQKSYNREALLEKRKKIQDEIKKKSNSLQKIESIRWD
AAKIQENKQQIRLKKVHLEKTTDNIEILIDYLYKKFFLXXXXXXXXIWNSRRVQGIYG
GLTFMHYTRKNKSVLIITSTFPGIELAD"
assembly_gap 155335..155354
/estimated_length=20
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 156654..157922
/gene="Gts1"
/locus_tag="P301_G10566"
mRNA 156654..157922
/gene="Gts1"
/locus_tag="P301_G10566"
/product="Gts1p"
CDS 156654..157922
/gene="Gts1"
/locus_tag="P301_G10566"
/note="Similar to YGL181W 'Arf3p GTPase Activating Protein
(GAP) that localizes to endocytic patches; gts1 mutations
affect budding, cell size, heat tolerance, sporulation,
life span, ultradian rhythms; localizes to nucleus and
induces flocculation when overexpressed'"
/codon_start=1
/product="Gts1p"
/protein_id="EWG90853.1"
/translation="MRFRSSSHSLKHVDRELKDLINSSENANKCGECGNFYPTWCSVN
LGVFLCGRCASVHRKVFGSRDDDAFSNVKSLSMDRWTREDIDELVSLGGNKGNARFWN
PKNVPFPFDGDDDKAIVEHYIRDKYILGKFRYDEIKPEDFGSRMDDFDGESDRFDERN
RSRSRSRSHSFYKRGHNRSDYGGSRDSFQSSGSRYSRQLAELKDMGFGDTNKNLDALS
SAHGNINRAIDYLESSSSRNSVSAAATTSTPPLPRRRATTSGPQPAIFVWYKCNHARF
YFKFSIFCTSKASSFRWYPSTVLRSCYRNDICRSAAIRHGYATAATATATACCRTGSG
SGTGTGSSSGSGSGSGPGSGSSTADPDATASDAATPTGAIIFSATGAIIFSANVARRK
PATRLFLHTIKIRYIAAAPHYISYRVTLSN"
gene 158829..161522
/gene="Atg1"
/locus_tag="P301_G10576"
mRNA 158829..161522
/gene="Atg1"
/locus_tag="P301_G10576"
/product="Atg1p"
CDS 158829..161522
/gene="Atg1"
/locus_tag="P301_G10576"
/note="Similar to YGL180W 'Protein ser/thr kinase required
for vesicle formation in autophagy and the
cytoplasm-to-vacuole targeting (Cvt) pathway; structurally
required for phagophore assembly site formation; during
autophagy forms a complex with Atg13p and Atg17p'"
/codon_start=1
/product="Atg1p"
/protein_id="EWG90854.1"
/translation="MGDIKNKDHTTSVNHNLMASAGNYTAEKEIGKGSFATVYRGHLT
SDKSQHVAIKEVSRAKLKNKKLLENLEIEIAILKKIKHPHIVGLIDCERTSTDFYLIM
EYCALGDLTFLLKRRKELMENHPLLRTVFEKYPPPSENHNGLHRAFVLSYLQQLASAL
KFLRSKNLVHRDIKPQNLLLSTPLIGYHDSKSFHELGFVGIYNLPILKIADFGFARFL
PNTSLAETLCGSPLYMAPEILNYQKYNAKADLWSVGTVVFEMCCGTPPFRASNHLELF
KKIKRANDVITFPSYCNIEPELKELICSLLTFDPAQRIGFEEFFANKVVNEDLSSYEL
EDDLPELESKSKGIVESNMFVSEYLSKQPKSPNSNLAGHQSMADNPAELSDALKNSNI
LTAPAVKTDHTQAVDKKASNNKYHNSLVSDRSFEREYVVVEKKSVEVNSLADEVAQAG
FNPNPIKHPTSTQNQNVLLNEQFSPNNQQYFQNQGENPRLLRATSSSSGGSDGSRRPS
LVDRRLSISSLNPSNALSRALGIASTRLFGGANQQQQQQQITSSPPYSQTLLNSQLFH
ELTENIILRIDHLQHPETLKLDNTNIVSILESLAAKAFVVYSYAEVKFSQIVPLSTTL
KGMANFENRRSMDSNAIAEEQDSDDAEEEDETLKKYKEDCLSTKTFGKGRTLSATSQL
SATFNKLPRSEMILLCNEAIVLYMKALSILSKSMQVTSNWWYESQEKSCSLRVNVLVQ
WLREKFNECLEKADFLRLKINDLRFKHASEVAENQTLEEKGSSEEPVYLEKLLYDRAL
EISKMAAHMELKGENLYNCELAYATSLWMLETSLDDDDFTNAYGDYPFKTNIHLKSND
VEDKEKYHSVLDENDRIIIRKYIDSIANRLKILRQKMNHQN"
gene complement(162174..163856)
/gene="Tos3"
/locus_tag="P301_G10581"
mRNA complement(162174..163856)
/gene="Tos3"
/locus_tag="P301_G10581"
/product="Tos3p"
CDS complement(162174..163856)
/gene="Tos3"
/locus_tag="P301_G10581"
/note="Similar to YGL179C 'Protein kinase, related to and
functionally redundant with Elm1p and Sak1p for the
phosphorylation and activation of Snf1p; functionally
orthologous to LKB1, a mammalian kinase associated with
Peutz-Jeghers cancer-susceptibility syndrome'"
/codon_start=1
/product="Tos3p"
/protein_id="EWG90855.1"
/translation="MVLLKEPVQPLPRSSLLYNNASNSSSRIKETRKVKLLYNPLTKR
QILNNFEILATLGNGQYGKVKLARDLGTGALVAIKILNRFEKRSGYSLQLKVENPRVN
QEIEVMKRCHHENVVELYEILNDPESTKVYLVLEYCSRGPVKWCPENKMEIKAVGPSI
LTFQQSRKVVLDVVSGLEYLHSQGITHRDIKPSNLLISSNGTVKISDFGVAMSTATGS
TNIQSSHEQLLKSRALGTPAFFAPELCSTEKEYSCSSAIDIWSLGVTIYCLLFGKLPF
NANSGLELFDSIINKPLEFPSYEEMLNGATSGITMEEYTDAKDLLKKLLQKDPDKRIK
LADIKVHPFMCHYGKSDAASVSTNLETFHELKVSPPSSCKRVELVSLPVNSSFASLDS
VYMENFDHNNLRTGADRNSTYSPSIYDANTLSPSAYHNIGSRESSYSSFSSFTSSTAF
ASQISIQDAPAIGDQQCLIGESGSSLRVNSCEFPQYTTMSPVGEYPFESTEASLSSTL
TPVGNVPQRIKAHLVEGKSNSKDDLRIEADASLVFEASDAQRTRRRMSLYKL"
assembly_gap 164955..164968
/estimated_length=14
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 166131..169405
/gene="Mpt5"
/locus_tag="P301_G10591"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 168019..168033
/estimated_length=15
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(168805..170469)
/locus_tag="P301_G10601"
mRNA complement(168805..170469)
/locus_tag="P301_G10601"
/product="hypothetical protein"
CDS complement(168805..170469)
/locus_tag="P301_G10601"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90856.1"
/translation="MTPNLPGFYYDRERRRYFRISENQSISTTGTTNQYRKDNIKRQC
VEENYDKKFSMIKKKRQQTLQKYKLSLLNPLERAFRPLSYEKYMIGLNMQYASHSLTE
GHHSHSSANVKSLNFPHRIQIGVLANCILLVTQEGCFHSKLVFATNKGYVAGFSSLDN
FPEENFFTGFSMAELNPMLKYKSEPTDVFKTMKLERTVAIKEGPSHYFYHNVNTRSNV
HTFAIFIQDFSSLKLLKIRQVKLKENCQVHDSLVVGDTLIITVNYRCHFYDLIPETFP
NPYIFSPAKSSRKHKSRSDITSLSFCLQEDALSPLKKTNTGVFYLGYRNGDSMAIVFT
NITNMTLQYSKTNGMASESRNQPIRNSPKSVVSIKALNNKGLMLISGMADKEDVQQLV
IADTFLEDILTEIPVVSFKTKFLNVTKDTEILEISDDGRYFIYGSTSARDGKGDFEVF
CTTLSGNLDYEKSEGGNITLYPIGGMKNYCRLENFQFESIHLHSAFIPPRYVKLFDAV
EPLGEESSTSPYDIPEEGLSQKICILIRREDDPYNGANIFITSALT"
gene complement(170674..171711)
/gene="Sae2"
/locus_tag="P301_G10606"
mRNA complement(170674..171711)
/gene="Sae2"
/locus_tag="P301_G10606"
/product="Sae2p"
CDS complement(170674..171711)
/gene="Sae2"
/locus_tag="P301_G10606"
/note="Similar to YGL175C 'Endonuclease that processes
hairpin DNA structures with the MRX complex; involved in
meiotic and mitotic double-strand break repair;
phosphorylated in response to DNA damage and required for
normal resistance to DNA-damaging agents'"
/codon_start=1
/product="Sae2p"
/protein_id="EWG90857.1"
/translation="MVTGEENVYLKSSLSILKELSLDELLNVQYDVTTLIAKRVQALQ
NRNKCVLEEPNSKLAEILCHEKNAPQQSSQTSAGPGEQDSEDFILTQFDEDIKKESAE
VHYRNENKHTVQLPLVTMPPNRHKRKISEFSSPLNGLNNLSDLEDCSDTVIHEIDNDK
ENKTRKLLGIELENPGSTSPNLYKNVKDNFLFDFNTNPLTKRAWILEDFRPNEDIAPV
KRGRRKLERFYAQVGKPEDSKHRSLSVVIESQNSDYEFAFDNLRNRSKSPPGFGRLDF
PSTQEGNEDKKKSQEIIRRKTKYRFLMASNNKIPPYEREYVFKREQLNQIVDDGCFFW
SDKLLQIYARC"
gene 171933..172733
/gene="Bud13"
/locus_tag="P301_G10611"
mRNA 171933..172733
/gene="Bud13"
/locus_tag="P301_G10611"
/product="Bud13p"
CDS 171933..172733
/gene="Bud13"
/locus_tag="P301_G10611"
/note="Similar to YGL174W 'Subunit of the RES complex,
which is required for nuclear pre-mRNA retention and
splicing; involved in bud-site selection; diploid mutants
display a unipolar budding pattern instead of the
wild-type bipolar pattern'"
/codon_start=1
/product="Bud13p"
/protein_id="EWG90858.1"
/translation="MALHQYLSETYGPTKPKNKTKKKKKESKSDANSDKTSLIVKERL
STLQQEQEKSGVASFSKFDKQKSKNIWKNLETNELSHAITHPSASSIAGNESKNDLKE
IRAQEPLVTVADKSKTRKTIYRDAQGHKIQEDSKIDDSSFSQSKYEDEKAAEREQYLK
NLNMGDVQKLGINVDAHDKKKNQTASSLTIEDPAITFTHDKERTVKTSLLGRKLYDKP
APENRFAIMPGSRWDGVHRSNGFEEKWFAKQNEINEKKVQSYTLQEDY"
gene complement(172915..177501)
/gene="Kem1"
/locus_tag="P301_G10616"
mRNA complement(172915..177501)
/gene="Kem1"
/locus_tag="P301_G10616"
/product="Kem1p"
CDS complement(172915..177501)
/gene="Kem1"
/locus_tag="P301_G10616"
/note="Similar to YGL173C 'Evolutionarily-conserved 5'-3'
exonuclease component of cytoplasmic processing (P) bodies
involved in mRNA decay; plays a role in
microtubule-mediated processes, filamentous growth,
ribosomal RNA maturation, and telomere maintenance'"
/codon_start=1
/product="Kem1p"
/protein_id="EWG90859.1"
/translation="MGIPKFFRYISERWPMILQLIEGTQIPEFDNLYLDMNSILHNCT
HGNDDDVTKRLTEEEVFAKICTYIDHLFQTIKPKKIFYMAIDGVAPRAKMNQQRARRF
RTAMDAEKALKKAIENGDEIPKGEPFDSNSITPGTEFMAKLTKNLQYFIHDKISNDSK
WREVQIIFSGHEVPGEGEHKIMNFIRHLKSQKDFNQNTRHCIYGLDADLIMLGLSTHG
PHFALLREEVTFGRRNSEKKSLEHQNFYLLHLSLLREYMELEFKEIADEMQFEYNFER
ILDDFILVMFVIGNDFLPNLPDLHLNKGAFPVLLQTFKEALLHTDGYINEHGKINLKR
LGVWLNYLSQFELLNFEKDDIDVEWFNKQLENISLEGERKRQRVGKKLLVKQQKKLIG
SIKPWLMEQLQEKLSPDLPDEEIPTLELPKDLDMKDHLEFLKEFAFDLGLFITHSKSK
GSYSLKMDLDSINPDETEEEFQNRVNSIRKTIKKYQNAIIVEDKEELETEKTIYNERF
ERWKHEYYHDKLKFTTDSEEKVRDLAKDYVEGLQWVLYYYYRGCPSWSWYYPHHYAPR
ISDLVKGLDQDIEFDLSKPFTPFQQLMAVLPERSKNLIPPTFRPLMYDEQSPIHDFYP
AEVQLDKNGKTADWEAVVLISFVDEKRLIEAMQPYLCKLSPEEKTRNQFGKDLIYSFN
PQVDNIYKSPLGGIFSDIEHNHCVEKEYITIPLDSSEIRYGLLPNAKLGAEMLAGFPT
LLSLPFTSSLEYNETMVFQQPSKQQSMVLQITDIYKTNNVTLEDFSKRHLNKVIYTRW
PYLRESKLVSLTDGKTIYEYQESNDKKKFRFITKPAETQDKKLFNSLKNSMLRMYAKQ
KAVKIGPVEAIATVFPVTGLVRDSDGGYIKTFSPTPDYYPLQLVVESVVNEDERYKER
GPIPIEEEFPLNSKVIFLGDYAYGGETTIDGYSSDRRLKITVEKKFLDSEPTIGKERL
QMDHQAVKYYPSYIVAKNMHLHPLFLSKITSKFMITDATGKHINVGIPVKFEARHQKV
LGYARRNPRGWEYSNLTLNLLKEYRQTFPDFFFRLSKVGNDIPVLEDLFPDTSTKDAM
NLLDGIKQWLKYVSSKFIAVSLESDSLTKTSIAAVEDHIMKYAANIEGHERKQLAKVP
REAVLNPRSSFALLRSQKFDLGDRVVYIQDSGKVPIFSKGTVVGYTTLSSSLSIQVLF
DHEIVAGNNFGGRLRTNRGLGLDASFLLNITNRQFIYHSKASKKALEKKKQSNNRNNN
TKTAHKTPSKQQSEEKLRKERAHDLLNFIKKDTNEKNSESVDNKSMGSQKDSKPAKKV
LLKRPAQKSSENVQVGLANFEKAPLDNPTVAGSIFNAVANQYSDGIGSNLNIPTPPHP
MNVVGGPIPGANDVADVGLPYNIPPGFMTHPNGLHPLHPHQMPYPNMNGMSIPPPAPH
GFGQPIPFPPPPPMTNVSDQGSRIVVNEKESQDLKKFINGKQHSNGSTIGGETKNSRK
GEIKPSSGTNSTECQSPKSQSNAADRDNKKDEST"
gene 178089..179507
/gene="Nup49"
/locus_tag="P301_G10621"
mRNA 178089..179507
/gene="Nup49"
/locus_tag="P301_G10621"
/product="Nup49p"
CDS 178089..179507
/gene="Nup49"
/locus_tag="P301_G10621"
/note="Similar to YGL172W Subunit of the
Nsp1p-Nup57p-Nup49p-Nic96p subcomplex of the nuclear pore
complex (NPC), required for nuclear export of ribosomes"
/codon_start=1
/product="Nup49p"
/protein_id="EWG90860.1"
/translation="MFGLNKASSTPAGGLFGQASGASTGNANTGFSFGGTQTGQNTGP
STGGLFGAKPAGSTGGLGASFGQQQQQSQTNAFGGSATTGGGLFGNKPNNTANTGGGL
SGANSNSNSGGLFGSNNAQTSGGLFGNNNTNNINNSSSGMNNASTGLFGSKPAGGTSL
FGNTSTSSAPAQNQGMFGAKPAGTSLFGNNAGNTTTGGGLFGSKPTGATSLFGSSNNN
NNNNNSNNIMSASGGLFGNQQQQLQQQPQMQCALQNLSQLPITPMTRISELPPQIRQE
IEQLDQYIQKQVQISHHLKADTIDHDELIDSIPRDVAYLLKSESATSQYLKQDLKKIS
SFKSLIDEDLLDTQTFSVLLQQLLTPGSKISSNDLDKFFQKKIHLYEKKLEDYCRILS
DIETAVNGIDTDLFGAPNNPNSTAITADLGSSEAENLLQLKTGLAAIVSTVIEEFTLF
MDIAERIAVLHQKTKTLASLSI"
gene 179779..181473
/gene="Rok1"
/locus_tag="P301_G10626"
mRNA 179779..181473
/gene="Rok1"
/locus_tag="P301_G10626"
/product="Rok1p"
CDS 179779..181473
/gene="Rok1"
/locus_tag="P301_G10626"
/note="Similar to YGL171W 'ATP-dependent RNA helicase of
the DEAD box family; required for 18S rRNA synthesis'"
/codon_start=1
/product="Rok1p"
/protein_id="EWG90861.1"
/translation="MDIFRVLTRGASVKKESGPKAKAADYSVINGKDENHKEDNNESQ
IVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRK
SYKGNVSGIDIPLPIGSFEDLISRFSFDRRLLNNLIENGFTEPTPIQCECIPVALNNR
DVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSHK
IFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKH
LIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVI
IGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHEL
MYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVP
GSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKM
TRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK"
gene complement(181542..182784)
/gene="Spo74"
/locus_tag="P301_G10631"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(183104..183176)
/locus_tag="P301_G10636"
tRNA complement(183104..183176)
/locus_tag="P301_G10636"
/product="tRNA-Lys"
/note="TK(CUU)G2"
gene 183448..184728
/gene="Sua5"
/locus_tag="P301_G10641"
mRNA 183448..184728
/gene="Sua5"
/locus_tag="P301_G10641"
/product="Sua5p"
CDS 183448..184728
/gene="Sua5"
/locus_tag="P301_G10641"
/note="Similar to YGL169W 'Single-stranded telomeric
DNA-binding protein, required for normal telomere length;
null mutant lacks N6-threonylcarbamoyl adenosine (t6A)
modification in the anticodon loop of ANN-decoding tRNA;
member of conserved YrdC/Sua5 family'"
/codon_start=1
/product="Sua5p"
/protein_id="EWG90862.1"
/translation="MYLGRHFLAMTSKALFDTKILKVNPLSIIFSPDAHIDGSLPTIT
DPETEAALVEAARIIRDTDETVAFPTETVYGLGGSALNDNSVLSIYKAKNRPSDNPLI
THVSSIDQLNRKVFNQPHLSGTSLFDNIPSIYRPLISSLWPGPLTILLPVPSSEHSAL
SKLTTADQPTFAVRIPANPVARALIALSDTPIAAPSANASTRPSPTLASHVYHDLKDK
IPIILDGGACKVGVESTVVDGLCNPPMLLRPGGFTYEEIVKLGGEAWSLCKVENKKTV
EKGEKVRTPGMKYRHYSPSAKVVLLVPHCEGDGILKGVDRMERLKRLIETELKANRNI
KKIAILTSLKLRDSDLQSKIFNEPDFSSKTFIIERLGQSGEEIQTNLFAALRKVDEND
KVDLIFVEGINEEGEGLAVMNRLRKAAANNCIQF"
gene 184820..185152
/gene="Hur1"
/locus_tag="P301_G10646"
mRNA 184820..185152
/gene="Hur1"
/locus_tag="P301_G10646"
/product="Hur1p"
CDS 184820..185152
/gene="Hur1"
/locus_tag="P301_G10646"
/note="Similar to YGL168W 'Protein of unknown function;
reported null mutant phenotype of hydroxyurea sensitivity
may be due to effects on overlapping PMR1 gene'"
/codon_start=1
/product="Hur1p"
/protein_id="EWG90863.1"
/translation="MFILVSVVNICTYIHLHMFPLIRTFTSIGLGVLMKDKGKEGKTI
KAQNVTYQTFEKYVESSSFFFLVHNFLSSSTMKTLLLMSNNNSISEIPSFSVLKILWK
NGIYIAHI"
gene complement(184972..187824)
/gene="Pmr1"
/locus_tag="P301_G10651"
mRNA complement(184972..187824)
/gene="Pmr1"
/locus_tag="P301_G10651"
/product="Pmr1p"
CDS complement(184972..187824)
/gene="Pmr1"
/locus_tag="P301_G10651"
/note="Similar to YGL167C 'High affinity Ca2+/Mn2+ P-type
ATPase required for Ca2+ and Mn2+ transport into Golgi;
involved in Ca2+ dependent protein sorting and processing;
mutations in human homolog ATP2C1 cause acantholytic skin
condition Hailey-Hailey disease'"
/codon_start=1
/product="Pmr1p"
/protein_id="EWG90864.1"
/translation="MSDNPFNASLLDEDSNREREILDATAEALSKPSPSLEYCTLSVD
ETLEKLDTDKNGGLRSSNEANNRRSLYGPNEITVEDDESLFKKFLSNFIEDRMILLLI
GSAVVSLFMGNIDDAVSITLAIFIVVTVGFVQEYRSEKSLEALNKLVPAECHLMRCGQ
ESHVLASTLVPGDLVHFRIGDRIPADLRIIEAIDLSIDESNLTGENEPVHKTSQTIEK
SSFNDQPNSIVPISERSCIAYMGTLVKEGHGKGIVVGTGTNTSFGAVFEMMNNIEKPK
TPLQLTMDKLGKDLSLVSFIVIGMICLVGIIQGRSWLEMFQISVSLAVAAIPEGLPII
VTVTLALGVLRMAKRKAIVRRLPSVETLGSVNVICSDKTGTLTSNHMTVSKLWCLDSM
SNKLNVLSLDKNKKTKNSNGNMKNYLTEDVRETLTIGNLCNNASFSQEHAIFLGNPTD
VALLEQLANFEMPDIRNTVQKVQELPFNSKRKLMATKILNPVDNKCTVYVKGAFERIL
EYSTSYLKSKGKKTEKLTEAQKATINECANSMASEGLRVLGFAKLTLSDSSTPLTEDL
IKDLTFTGLIGMNDPPRPNVKFAIEQLLQGGVHIIMITGDSENTAVNIAKQIGIPVID
PKLSVLSGDKLDEMSDDQLANVIDHVNIFARATPEHKLNIVRALRKRGDVVAMTGDGV
NDAPALKLSDIGVSMGRIGTDVAKEASDMVLTDDDFSTILTAIEEGKGIFNNIQNFLT
FQLSTSVAALSLVALSTAFKLPNPLNAMQILWINILMDGPPAQSLGVEPVDHEVMKKP
PRKRTDKILTHDVMKRLLTTAACIIVGTVYIFVKEMAEDGKVTARDTTMTFTCFVFFD
MFNALACRHNTKSIFEIGFFTNKMFNYAVGLSLLGQMCAIYIPFFQSIFKTEKLGISD
ILLLLLISSSVFIVDELRKLWTRKKNEEDSTYFSNV"
gene 188483..189160
/gene="Cup2"
/locus_tag="P301_G10656"
mRNA 188483..189160
/gene="Cup2"
/locus_tag="P301_G10656"
/product="Cup2p"
CDS 188483..189160
/gene="Cup2"
/locus_tag="P301_G10656"
/note="Similar to YGL166W 'Copper-binding transcription
factor; activates transcription of the metallothionein
genes CUP1-1 and CUP1-2 in response to elevated copper
concentrations'"
/codon_start=1
/product="Cup2p"
/protein_id="EWG90865.1"
/translation="MVVINGVKYACETCIRGHRAAQCTHTDGPLQMIRRKGRPSTTCG
HCKELRRTKNFNPSGGCMCASARRPAVGSEEDETRCRCDEGEPCKCHTKRKSSRKSKG
GSCHRRANDEAAHVNGLGIADLDVLLGLNGRSSDVDMTTTLPSLKPPLQNGEIKADSI
DNLDLASLDPLEQSPSISMEPVSINETGSAYTTTNTALNDIDIPFSINELNELYKQVS
SHNSHSQ"
gene complement(189349..190653)
/gene="Yrb30"
/locus_tag="P301_G10666"
mRNA complement(189349..190653)
/gene="Yrb30"
/locus_tag="P301_G10666"
/product="Yrb30p"
CDS complement(189349..190653)
/gene="Yrb30"
/locus_tag="P301_G10666"
/note="Similar to YGL164C 'RanGTP-binding protein,
inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP
(Gsp1p); shares similarity to proteins in other fungi but
not in higher eukaryotes'"
/codon_start=1
/product="Yrb30p"
/protein_id="EWG90866.1"
/translation="MDEILAKAGSQAVTFAIKSGISIASTYALKTITNFVVQIPKDDA
RRIDQLKFKLESRMAIVSSAIDLIKLVAARGNTNLQITLRLTKDLKEEIDRFDEKINE
MTQKVEGSRSAKTQNEAIKAVENYIKDLLLRIEEITPFINLSLTTSGANLNSALPYQL
SPGLLLKASDFVSENNRKYEKAMKSNEKGTGDKEILKVQVGPTFEVTLFSIFYNLTSE
NNGQSGIVWKEDMKRAKARIYRLNSTGRKYDYFMKIEQDFNDGRYHEDDDKEDTPQEL
AIDLNHIKKLFFSVSGKLLRLEEQDSPVLVLKIDRSDDKENESSEGDKGLLDDITWYA
VSGYEAIEEDEEEDEEEDEEEGKDGEERKEEEEEENKLEDKDSSITLLEYIIRLTSLQ
SNDQKSILEVSDERLSIYLNDENTNSRKDRISNSTIEETEKS"
gene complement(191061..193757)
/gene="Rad54"
/locus_tag="P301_G10676"
mRNA complement(191061..193757)
/gene="Rad54"
/locus_tag="P301_G10676"
/product="Rad54p"
CDS complement(191061..193757)
/gene="Rad54"
/locus_tag="P301_G10676"
/note="Similar to YGL163C 'DNA-dependent ATPase,
stimulates strand exchange by modifying the topology of
double-stranded DNA; involved in the recombinational
repair of double-strand breaks in DNA during vegetative
growth and meiosis; member of the SWI/SNF family'"
/codon_start=1
/product="Rad54p"
/protein_id="EWG90867.1"
/translation="MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPY
KNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQLTK
RRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGMKKKITPEPRP
LHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEER
PTQRIGRHPALMTNGVRNKSLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQV
EGVRFLYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMA
DEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLT
PLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVG
LMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPGLLGS
RAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCK
YEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFE
DEFDDEDDLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNYT
QTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGIN
LIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKM
SLSSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQLKRAPAM
LYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH"
gene 195465..196364
/gene="Sut1"
/locus_tag="P301_G10686"
mRNA 195465..196364
/gene="Sut1"
/locus_tag="P301_G10686"
/product="Sut1p"
CDS 195465..196364
/gene="Sut1"
/locus_tag="P301_G10686"
/note="Similar to YGL162W 'Transcription factor of the
Zn[II]2Cys6 family involved in sterol uptake; involved in
induction of hypoxic gene expression'"
/codon_start=1
/product="Sut1p"
/protein_id="EWG90868.1"
/translation="MSTSITVRNRDRSLPPLLLPNVSLLEKDIRRKGTQNVGITDPEL
LLTTWMRKRAFPTDELLGGYKRLKPAAADSNECAIGIATVTPPPTLPVSAIVPPPQNY
TPPLFEYHPHALASMVNEDANASCTQMSIISRSTSNSTTSSATSTSSISKRQRSGPSC
DKCRLKKIKCNAKIEILLQDDTIMPMISNKLRYVLTPDDIRLYRGTLLRNIAIPDDVI
EGTGSRKLIKHIDKLVLLTPCLPCIKKKHSSSSTNFPKNDKCTFSKGFTRADINISSK
ISLKFKDKTIYDITYDDYKSIDF"
gene complement(196537..197469)
/gene="Yip5"
/locus_tag="P301_G10691"
mRNA complement(196537..197469)
/gene="Yip5"
/locus_tag="P301_G10691"
/product="Yip5p"
CDS complement(196537..197469)
/gene="Yip5"
/locus_tag="P301_G10691"
/note="Similar to YGL161C 'Protein that interacts with Rab
GTPases, localized to late Golgi vesicles; computational
analysis of large-scale protein-protein interaction data
suggests a possible role in vesicle-mediated transport'"
/codon_start=1
/product="Yip5p"
/protein_id="EWG90869.1"
/translation="MPSNNSSFLDIDDDLEGVDDFGNEPNPFDDATVPDSPNMNNSTA
GKGREFYNTTGSKAESAPLQGQMDPPAYDQVIGQNDNDGLGRNGLRPGLINYYSKYFQ
IDLTQFKKRLSAVLTFRNDHNSESNEDNTDLYGAVWITATVVMINFTMSRGLNFIISD
VIEGVKTGEDIDRASQFKKLLHSIWLFYGYTFGVPFITMQVLNRDEHSERNRSFKSVP
ELISVYGYANLIWIPVCVILNILDMSKRLRTVQAIQWAIVALGWAQSSYFLNSQISSN
NNTETQSNGKFSLSIIVVVALHTLFCLLFRFIIF"
gene 197888..199600
/gene="Aim14"
/locus_tag="P301_G10696"
mRNA 197888..199600
/gene="Aim14"
/locus_tag="P301_G10696"
/product="Aim14p"
CDS 197888..199600
/gene="Aim14"
/locus_tag="P301_G10696"
/note="Similar to YGL160W 'Protein with similarity to
iron/copper reductases (FRE1-8), possibly involved in iron
homeostasis; may interact with ribosomes; null mutant
displays elevated frequency of mitochondrial genome loss'"
/codon_start=1
/product="Aim14p"
/protein_id="EWG90870.1"
/translation="MKESPLITLVKRHSETHFANIKYGYYVLIISLVYLIGLALLRAF
GRRTPSRSSSAFKNKIIYRLYDIDPAIHLGILFFAVLIPFYYHYSLTTQSTVYLKRLG
RLSYALIPLNLFLTLRPNWFLRKNCTYTDFIPFHKWFSRIITVIGLLHGIFFIIKWAI
DDNVSLKQKLILKTFNFVGFIISILVLFLLICSIGPMRRYNYRLFYIVHNLVNVAFIL
LTPIHSRPGVKFPFLLLNCTLLFIHIINRIVFAKSLMILNKNANYSKTNLVHVRLPRA
ILPDYFEPGSHIRISPYRRINPLYWLLPSHPYTIASLAEDNSIDLIIKETSTAEPGSQ
IESLRSNPKSFHLDQEKTYTLINSYPPSVPEECYSQGTNIAIICGGSGISFALPLFRH
FFNKENVKYLKMIWLIKNYSEYELVLDYLKTNGLTFEKKPSNNKRISVFISGEYTAET
RLDEITTNIDDENSEYEMGSFNNEDEDLSISNFNSENADSNDKTPETSHSPTKENGSL
IEVKSKHSFTLSNELKSFNNESAQVNQNETWLFSCGPPSLLQLSKKYCNDERINFVCE
TYGL"
gene 200048..201160
/locus_tag="P301_G10701"
mRNA 200048..201160
/locus_tag="P301_G10701"
/product="hypothetical protein"
CDS 200048..201160
/locus_tag="P301_G10701"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90871.1"
/translation="MNNSIITDDEVREFYLNCSSQTIIESLLSLHESLRLYSQNHEIL
LNRMFKKLDETNADSNISHIFMPVVSKDFSGIKILVNNNNKNFQGVINVIEPETGKLI
GCFEAKQITAIRTALASCIGLYKQLSCSHDKFFRFENGTCYLTCFGTGLQAFWHIYIA
IKLIMSGIVGESLKLVEINILYHNNMMSLDRLKSLKNLFGSNIKIELNQYQINDISSE
GNGAVSNSDIIFGCLPTLEPNLFLRQLLNSKASVEQKHTYISLIGSYKPVMHECDKEL
IDKFKSDNESACILVDSREHTLLESGELIDSNIAPHNLIEIGELDTLKDTVLNLNEKG
CKRTITLCKIVGLAVMDVALAKEFLSLRTKKTENKE"
gene 202713..203098
/locus_tag="P301_G10706"
tRNA 202713..203098
/locus_tag="P301_G10706"
/product="tRNA-Leu"
/note="TL(CAA)G1"
assembly_gap 202845..202971
/estimated_length=127
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 204360..205898
/gene="Rck1"
/locus_tag="P301_G10711"
mRNA 204360..205898
/gene="Rck1"
/locus_tag="P301_G10711"
/product="Rck1p"
CDS 204360..205898
/gene="Rck1"
/locus_tag="P301_G10711"
/note="Similar to YGL158W 'Protein kinase involved in the
response to oxidative stress; identified as suppressor of
S. pombe cell cycle checkpoint mutations'"
/codon_start=1
/product="Rck1p"
/protein_id="EWG90872.1"
/translation="MSVNPEFIADGIDFYPTTPDAAYFNAADGKNKVNRINGNSENLH
HSFASSCRRSSLSVDFNVTSSDSEKSEQSCLENNSQEDEYFCDIFSTELKLDETSNKS
TDYSSSNHQYPEQLELHNYKLLNKIGEGAFSRVFKAVGINTDDQAPVVIKAIIKKGIS
SDDTLKGNDRIQGSSRKKVLNEVAIHKLVSKNNPHCTKFIAXXXXXXXXXXXXXXXXX
GEIFDRIVQLTCFSEDLARHVITQVAIAIKHRHYMGIVHRDVKPENLLFEPIPFYGLD
GDMQKEDEFTLGVGGGGIGLVKLMDFGLAKKLRNNTAKTPCGTIEYVAPEVFTSKRYS
MKVDMWSIGCVLFTLLCGYPPFYEKNEKTLLKKISRGDYEFLAPWWDNISSGAKNAVT
HLLEVDPNKRYDIDDFLNDPWLNSYDCLKDSNSNSYASVQSILNDSFDERAETLHCAL
SCQSEKQDDTEFSRSESSEYIFMTEEDRNLRGSWIGEPKECFTLDLATSSIYRRRKNM
IFFW"
assembly_gap 204964..205011
/estimated_length=48
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 206353..207396
/locus_tag="P301_G10716"
mRNA 206353..207396
/locus_tag="P301_G10716"
/product="hypothetical protein"
CDS 206353..207396
/locus_tag="P301_G10716"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90873.1"
/translation="MTTDTTVFVSGATGFIALHIVNDLLKAGYTVIGSGRSQEKNDGL
LKKFNNNPKLSMEIVEDIAAPNAFDEVFKKHGKEIKIVLHTASPFHFETTNFEKDLLT
PAVNGTKSILEAIKKYAADTVEKVIVTSSTAALVTPTDMNKGDLVITEESWNKDTWDS
CQANAVAAYCGSKKFAEKTAWEFLKENKSSVKFTLSTINPGFVFGPQMFADSLKHGIN
TSSGIVSELIHSKVGGEFYNYCGPFIDVRDVSKAHLVAIEKPECTGQRLVLSEGLFCC
QEIVDILNEEFPQLKGKIATGEPATGPSFLEKNSCKFDNSKTKKLLGFQFYNLKDCIV
DTAAQMSEVQNEA"
gene 207763..211014
/gene="Ams1"
/locus_tag="P301_G10721"
mRNA 207763..211014
/gene="Ams1"
/locus_tag="P301_G10721"
/product="Ams1p"
CDS 207763..211014
/gene="Ams1"
/locus_tag="P301_G10721"
/note="Similar to YGL156W 'Vacuolar alpha mannosidase,
involved in free oligosaccharide (fOS) degradation;
delivered to the vacuole in a novel pathway separate from
the secretory pathway'"
/codon_start=1
/product="Ams1p"
/protein_id="EWG90874.1"
/translation="MSSEDIIYDPQFKPVQGIYENRLRQFIDTGGDYHDLNLPKFYDK
KRISLDHDHVKVWWYQVSFERGSSPVSPDKRPSWKSIIERDKKGELEFREANINQPFG
PSWSTTWFKVKISLPEDWVKSDEQLLFQWDCSNEGIVIDPKTLIPVTAFSGGERTEYV
LPKTSDGKHFFYIEAGNNGMFGCGAGSTINPPDDNRFFHLRKADIVWPDLDARALYID
FWMLGDAARELPGDSWQKHQARQLGNAVMNLFDPNDRSSVRKCRELLQREYFDSFLES
SKVYEQGESQVLTNVYGIGNCHIDTAWLWPFAETRRKIVRSWSSQCTLMDRFPEYKFV
ASQAQQFKWLLEDHPEFFNKVLIPKIQQSQFFAVGGTWVENDTNIPSGESLARQFFFG
QRFFLKHFGLKSKIFWLPDTFGYSSQMPQLCRLSGIDKFLTQKLSWNNINSFPHSTFN
WAGIDGSQLLTHMPPGNTYTADSHFGDVLRTAKQNKTPECYGSGLMLYGKGDGGGGPT
EEMLQKMRRIRSMNNRNGNVIPKLQVGITVDEFYDDILKRTNQGHDLPTWSGELYFEF
HRGTYTSQAQTKKLMRLSEIKLHDLEWIAAKTSVLYPDSYKYPSKQINELWENVLLCQ
FHDVLPGSCIEMVYKYEAVPMLHNVVKECTSLIDKTVQFLQSQSKADLVEMRTLTWSK
PEKVSEECSLNGSYTSSVTGYDDYIVLANGKLKVIICKKTGVITSVTDETLGVEYLDT
EHGRNKLGANQFVIFDDKPLGWQAWDTELYSVNQYKYVTKPKKVQVSCNTKEKCAVEV
IFQISEKCKIKSVISLNATAVTDAKLSKVDISTTVENWDARNKFLKVEFPVNIRNDFA
SYETQFGITKRPTHYNTSWDVAKFEVCHHKFADYSEYSKGVSILNNCKYGFSTHGNLM
RLSLLRSPKAPDAHADMGTHEIKYAIYPHRGALSSDTVKLAHEFNYCFKYKLPKDIGM
NFDDIISISGDENVILSNIKRGEDDSAVKSNYSLNPRDEQSIVVRVYESLGGESFASL
NTTLNLKRIEKVDNLEMKVYKSLTATRDESNHAINRIPIKLRPFEIASFRLYF"
assembly_gap 211111..211229
/estimated_length=119
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 211433..212563
/gene="Cdc43"
/locus_tag="P301_G10731"
mRNA 211433..212563
/gene="Cdc43"
/locus_tag="P301_G10731"
/product="Cdc43p"
CDS 211433..212563
/gene="Cdc43"
/locus_tag="P301_G10731"
/note="Similar to YGL155W 'Beta subunit of
geranylgeranyltransferase type I, catalyzes
geranylgeranylation to the cysteine residue in proteins
containing a C-terminal CaaX sequence ending in Leu or
Phe; has substrates important for morphogenesis'"
/codon_start=1
/product="Cdc43p"
/protein_id="EWG90875.1"
/translation="MCQATNGPSRVVTKKHRKFFERHLQLLPSSHQGHDVNRMAIIFY
SISGLSIFDVNVSAKYGDHLGWMRKHYIKTVLDDTENTAISGFVGSLVMNIPHATTIN
LPNTLFALLSMIMLRDYEYFETILDKRSLARFVSKCQRPDRGSFVSCLDYKTNCGSSV
DSDDLRFCYIAVAILYICGCRSKEDFDEYIDTEKLLGYIMSQQCYNGAFGAHNEPHSG
YTSCALSTLALLSSLEKLSDKFKEDTITWLLHRQVSSHGCMKFESELNASYDQSDDGG
FQGRENKFADTCYAFWCLNSLHLLTKDWKMLCQTELVTNYLLDRTQKTLTGGFSKNDE
EDADLYHSCLGSAALALIEGKFNGELCIPQEIFNDFSKRCCF"
gene complement(212629..213447)
/gene="Lys5"
/locus_tag="P301_G10736"
mRNA complement(212629..213447)
/gene="Lys5"
/locus_tag="P301_G10736"
/product="Lys5p"
CDS complement(212629..213447)
/gene="Lys5"
/locus_tag="P301_G10736"
/note="Similar to YGL154C 'Phosphopantetheinyl transferase
involved in lysine biosynthesis; converts inactive
apo-form of Lys2p (alpha-aminoadipate reductase) into
catalytically active holo-form by posttranslational
addition of phosphopantetheine'"
/codon_start=1
/product="Lys5p"
/protein_id="EWG90876.1"
/translation="MVKTIEVVSEVSKVAGVRPWAGIFVVEIQEDILADEFTFEALMR
TLPLASQARILNKKSFHDKCSSLCNQLLQLFGCSIVTGLNFQELKFDKGSFGKPLLDN
NRFLPFSMTIGEQYVAMFLVKCVSTDEYQDVGIDIASPCNYGGREELELFKEVFSERE
FNGLLKASDPCTIFTYLWSLKESYTKFTGTGLNTDLSLIDFGAISFFPAEGASMCITL
DEVPLIFHSQWFNNEIVTICMPKSISDKINTNRPKLYNISLSTLIDYFIENDGL"
gene 213624..214649
/gene="Pex14"
/locus_tag="P301_G10741"
mRNA 213624..214649
/gene="Pex14"
/locus_tag="P301_G10741"
/product="Pex14p"
CDS 213624..214649
/gene="Pex14"
/locus_tag="P301_G10741"
/note="Similar to YGL153W 'Peroxisomal membrane peroxin
that is a central component of the peroxisomal protein
import machinery; interacts with both PTS1 (Pex5p) and
PTS2 (Pex7p), peroxisomal matrix protein signal
recognition factors and membrane receptor Pex13p'"
/codon_start=1
/product="Pex14p"
/protein_id="EWG90877.1"
/translation="MSDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTE
KEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMAT
ATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFR
KKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDG
MQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGM
QEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNG
QVEDSIP"
gene 214872..218270
/gene="Nut1"
/locus_tag="P301_G10751"
mRNA 214872..218270
/gene="Nut1"
/locus_tag="P301_G10751"
/product="Nut1p"
CDS 214872..218270
/gene="Nut1"
/locus_tag="P301_G10751"
/note="Similar to YGL151W Component of the RNA polymerase
II mediator complex, which is required for transcriptional
activation and also has a role in basal transcription"
/codon_start=1
/product="Nut1p"
/protein_id="EWG90878.1"
/translation="MEKESVYNLALKCAERQLTSMEFSNLYKEFFNEKFPSLIQEEEE
DTTTTANINEVKKASDLVDTPSNNTAATADTTHLHEALDIFCSDFVKILNLEKPLILA
DYIVEVLLVNYNSDMIKCFLPKLNSVRNSLLLAHFFSKSCSFFAKLSDTLIIDQVRKD
LGNVIVPNILSLDMNSMNKELIAIVSKLLQTTLKLSPSPILLTSASCKNGSFTLLNQL
SQTNKLLFKRVSQTFEEKLHFKDTKPFLNKDSTNEFVGSPSLTSPQYIPSPLSSTKPP
GSVNSAAKYKDMKLLRYYKNIWLNNKIINWEISNPDFLSKYSAITSSIFQESFNSVQN
LDQLLTDLIETSFTCFAQFVSNKQYHQANSNLTLLERKWVIFITKHLPLLILENSSRS
PRVVTNALDNIDEKVVKAIRIYFTEKDDNKTNNEDLFDDYPSTSLDIRHDFIKGLIML
NLQPASVINNYLREDQMIDTSILPTSDDLVVRNLQGIQEVVHNTNSFIISSLDTLELE
SITESITHDSSNGLFQVLHNFESVAPTKQREIVKAFLSIFEDAIKELNYNRIAKICAL
LFFNFSHSLTTILSFSSPAALMKTLIKFVDLSRNGRNGSNGNDESSEYETINISLSFS
WAILLIINLTQTYGISVVDVALKYPELSIKNSFIINFISNLPNVSDKYYLEESNVNDS
DMLTKSHNTVQSWLCDLFVNGSITDQLIQNIETRQLANLIPFIVKQVLLSVEIGVLTD
ISSLIGGFEYFLQPLLLVGLIKTFYWLEQFLSCVKNDTISEDILQGIFNLLNTLFNPV
TLNEDSKAFHTAVLRLNAIPLLKVLRKFRVQSQSNYGIYSSDAQGDPNLEPLIAKLVA
VLNVSPVYDVDPRIINSENDYSRKQLGYGKFLILNENPINKIMTNQINSFWSLHSSTY
YNLDYLFELIELVTPKSFLFDVLKTLEYKLATYGVPGSENKRGSLDSEHVFDYFFYFL
VLYDVKTAEEASQLIEYMENDAKKSKGDVDIKGEDLHEKNDSAEVRQETQPKAEATQD
DDFDMLFGENDTSTQAYEEEEENEDNDGNNRTNNVPMIKAEETPSKTNKISILKRHSF
AVLLHERKLLNDLALENGEITKTENEKFISYHDKYLCMLKTCVF"
gene complement(218452..222920)
/gene="Ino80"
/locus_tag="P301_G10756"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 223746..224876
/gene="Aro2"
/locus_tag="P301_G10766"
mRNA 223746..224876
/gene="Aro2"
/locus_tag="P301_G10766"
/product="Aro2p"
CDS 223746..224876
/gene="Aro2"
/locus_tag="P301_G10766"
/note="Similar to YGL148W Bifunctional chorismate synthase
and flavin reductase, catalyzes the conversion of
5-enolpyruvylshikimate 3-phosphate (EPSP) to form
chorismate, which is a precursor to aromatic amino acids"
/codon_start=1
/product="Aro2p"
/protein_id="EWG90879.1"
/translation="MSTFGKLFRVTTYGESHCKSVGCIVDGVPPGMSLTEADIQPQLT
RRRPGQSKLSTPRDEKDRVEIQSGTEFGKTLGTPIAMMIKNEDQRPHDYSDMDKFPRP
SHADFTYSEKYGIKASSGGGRASARETIGRVASGAIAEKFLAQNSNVEIVAFVTQIGE
IKMNRDSFDPEFQHLLNTITREKVDSMGPIRCPDASVAGLMVKEIEKYRGNKDSIGGV
VTCVVRNLPTGLGEPCFDKLEAMLAHAMLSIPASKGFEIGSGFQGVSVPGSKHNDPFY
FEKETNRLRTKTNNSGGVQGGISNGENIYFSVPFKSVATISQEQKTATYDGEEGILAA
KGRHDPAVTPRAIPIVEAMTALVLADALLIQKARDFSRSVVY"
gene complement(225100..225677)
/gene="Rpl9a"
/locus_tag="P301_G10771"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(226099..227034)
/locus_tag="P301_G10776"
mRNA complement(226099..227034)
/locus_tag="P301_G10776"
/product="hypothetical protein"
CDS complement(226099..227034)
/locus_tag="P301_G10776"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90880.1"
/translation="MEKASLNIAQTKEIPLTDRRMGLRGWKACSRPHLLGRILLFMSI
LFITSAELSSDVSSREVYMPIFNNKLSFKVPPIKRSLLLGAALYEDFEYSSNNSASDG
AFCTVFNAGMNDASREVVFEIHVMDVLQEETDSSRFGGTSHERGRQSLGFSVFNNKNG
DLLRSKKNLASGTSVIEVNPGNCNEFLICFINLVYDGSWSSIDTEKSVTIKMTYNDKL
DPDMLLHLVNQMTPQVVKALNTVSDGLFQIVSDTTLLQMESDRRDINEATYSYLIVGF
VSLMVAQLISNIIVTTYLIIKIKSNPSSHIKKKGL"
gene 227589..229695
/gene="Tip20"
/locus_tag="P301_G10781"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 228855..228880
/estimated_length=26
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(229797..231854)
/gene="Rog1"
/locus_tag="P301_G10786"
mRNA complement(229797..231854)
/gene="Rog1"
/locus_tag="P301_G10786"
/product="Rog1p"
CDS complement(229797..231854)
/gene="Rog1"
/locus_tag="P301_G10786"
/note="Similar to YGL144C Protein with putative serine
active lipase domain"
/codon_start=1
/product="Rog1p"
/protein_id="EWG90881.1"
/translation="MSLTPTNEILFHYKSSVKVGELERYVITYHLYDGEEIPPDLNLN
SLWLKVRNMNPLSYRAAYLMGPFMLYCDVKTAQYHHSQKIVASVDYPKFEPNVQTQQD
FVAELSVHNIRQKYVWIADVMSQILFTTNTNVTYEVTIGTSKESVENPHDLPSHLGSY
SPKLTVNRLTTLDLWNLPVQITTPQKKKHLVVLTHGLHSNVSTDLVYIMEQIYKAQKN
YPHEQIVVKGYRGNVCQTEKGVKYLGTRLAEYIIQDLYDESIRKISFVGHSLGGLIQA
FAIAYIYEVYPWFFKKVNPINFITLASPLLGIVTDNPAYIKVLLSFGVIGKTGQDLGL
ENDVEVGKPLLYLLSGLPLIEILRRFKRRTVYANAINDGIVPLYTASLLFLDYNDILE
QLQKLKENSKKSPLINDASTPVNQDFFNKTFISPLTKMLSILAPQKFPTENGSEIPKV
SFFESASSILLPPLPERAYIMDPDSRDPVIIHDKIYNEDDIPQSEFDIEDGFFDKKNI
LLQAFFAGKKERAKYRNLEETIARRWHEGMAWRKVVVALKPDAHNNIIVRRKFANAYG
WPVIDHLIDVHFNGDDDDDNDENDDINSTQVVEPIQSVTEGKKKYRKAENIPQEYGWL
NKVETNGVFDEGPTGMISTVGEIVEALAKRGFSAVIDRRNASEDPNDEVLRFEEMNSD
LVQ"
gene complement(232064..233305)
/gene="Mrf1"
/locus_tag="P301_G10791"
mRNA complement(232064..233305)
/gene="Mrf1"
/locus_tag="P301_G10791"
/product="Mrf1p"
CDS complement(232064..233305)
/gene="Mrf1"
/locus_tag="P301_G10791"
/note="Similar to YGL143C 'Mitochondrial translation
release factor, involved in stop codon recognition and
hydrolysis of the peptidyl-tRNA bond during mitochondrial
translation; lack of MRF1 causes mitochondrial genome
instability'"
/codon_start=1
/product="Mrf1p"
/protein_id="EWG90882.1"
/translation="MWLSKFQFPSRSIFKGVFLGHKLPLLVRLTSTTTNSKSNGSIPT
QYTELSPLLVKQAEKYEAELKDLDKDLSCGIHFDVNKQKHYAKLSALTDTFIEYKEKL
NELKSLQEMIVSDPSLRAEAEQEYAELVPQYETTSSRLVNKLLPPHPFADKPSLLELR
PGVGGIEAMIFTQNLLDMYIGYANYRKWKYRIISKNENESGSGIIDAILSIEEAGSYD
RLRFEAGVHRVQRIPSTETKGRTHTSTAAVVVLPQIGDESAKSIDAYERTFKPGEIRV
DIMRASGKGGQHVNTTDSAVRLTHIPSGIVVSMQDERSQHKNKAKAFTILRARLAEKE
RLEKEEKERKARKSQVSSTNRSDKIRTYNFPQNRITDHRCGFTLLDLPGVLSGERLDE
VIEAMSKYDSTERAKELLESN"
gene complement(233616..235466)
/gene="Gpi10"
/locus_tag="P301_G10796"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 235700..238432
/gene="Hul5"
/locus_tag="P301_G10801"
mRNA 235700..238432
/gene="Hul5"
/locus_tag="P301_G10801"
/product="Hul5p"
CDS 235700..238432
/gene="Hul5"
/locus_tag="P301_G10801"
/note="Similar to YGL141W 'Multiubiquitin chain assembly
factor (E4); proteasome processivity factor that elongates
polyUb chains on substrates, opposing Ubp6p, a branched
polyubiquitin protease; required for retrograde transport
of misfolded proteins during ERAD'"
/codon_start=1
/product="Hul5p"
/protein_id="EWG90883.1"
/translation="MLNFTGQTRRRNVNLGNRTRNSKKDLLEKAKRERERRAQDKLKE
DASKTIQKSIRRHFSNVRLFKNTFTSSQLVHMIPAYGGKLIYYISQYDLQQLLKLSHN
FLSSYPNSLGNRQLLSLLKLYQDDALVAETLSDLNMDCPTVDEFLDSLSVYLCRASFL
SYSSASKLADVIEAWEVMHSSASISIFSISIGSYEKRPFALQFYCILAERNLLPQLIN
TNPILWDNMAKTYSHCSKGGQKNIAKLLIPNFNNHIAPSVLRSDNDYVLKFYEKAFID
EVIATTANYVSDEDHVKNLMCYIASSPNQSCKNSVLITLLSNKDFVRRLSWEFFHTKF
NASKTEAHPLFSVLAQLIDMHLLISTDRELLDYNSVIPIEELKKFTSTLKDFTFRQYW
ELPKSERNPMLKEAVPLLSKVYERDSRLHFLSTENNPTYWENSEKQFLNLRFYEELQE
YEDLYREHLEEESDEDMEKEIDLDKERPPLKSLLLNKMKKRLKSSLRFRKLEILLELP
FFIPFEERVDLFYMFIALDKKRLSLDDDHNLINMFTPWASTGMRKQSAIISRDNVLED
AFNAFNSIGERFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRT
NDRYELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLS
SFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEIDEPESSAKVVDLIPNGSKTYVT
KDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDN
IDLDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKAL
DPKFGIRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSGARFDLS"
gene complement(238700..242359)
/locus_tag="P301_G10806"
mRNA complement(238700..242359)
/locus_tag="P301_G10806"
/product="hypothetical protein"
CDS complement(238700..242359)
/locus_tag="P301_G10806"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90884.1"
/translation="MSLKSKLTKIQKLWLYYFPCDRILAKRICKSTVNTTVAFIFCLI
PKITAHLGAAPAMLPMISVIVHPGRRVGGTIHGAIYCITGLIFGLAYAIFGRFLAQRC
LGSSWHELTEAQQHVLHYKRYEAGLAILAVFEVIMLFFHGWMRSVSHYYFGIVFPLFV
VVHFAFMDPLNETAGTIAKAYSTPFYLGIAMSIFWNLVLFPEWGTTYLGNTTIDAMNE
LHKSIDYSINFFIAVDPHNSSQLYSRDPVSLGKLLKMKSLISSKVNNCRVVLHECIYE
FTYAYVSPTKLKPIISTLENLTVFINGLVNTCQLEFILLARHDNKLRPDDVAALTLPK
NKEISFANAEKLLKVIDKLHPVIYSLHRTMSECMYIAKLVLAHAFDVKVSRVHSCSMF
KDGNFPTFSNNANNLPNDVDIQNKINDLKQALEECKAKFKSEMLSFDIDIMSPSDEMF
LLSSFLLNFRQTADSTLVIMESVKDILVKRQIQEKKGWLRGKRLWFLVLTNYETFSIW
LKGDRNSVTENDTLKGTFNGNTNGFAHDTVIRRPDYEENELLSQKVSSNKNIVKDDAS
LDLPMTSEPKGNSSSTSDTSSSPLTLTKTTTFGTNRTSRRQGRFSFMSMLISIDKFCE
VSHPHFRFGFQVAIALMLASFPMFIPKTRQWYIDYRGTWIGFVCILCLEPSVGGTFWV
FFLRAVGVIFGAAWGYLSYVAAVNQTNPYLETVITVFGAIPGFYYLLGTPYVKAAIIE
IISIYIVMLAAILPSQDDILTSFAKRCLAVGYGGGVALIVQVFFFPLKAREQLNEEIS
FVCGCISEMELLYATGLEGEQVASSMSEEKYKKIEKISKSAKEALARATAYKGLTRQE
PRLKGEYTELENVFTQVIFIQKQIIERIDTISLLRKQNGSAVIEEFNSVVYPYRRQMV
GSISCLMRALQEAFINKTPLPQFLPSARIAHRRLINKVRQTLRIRYPGQISNLSDKAR
KPNEGDYADGKDEDDDDNEGLVMKMNRRGQANTTANPHEYVLKEKFLSWNASSAASEE
IIEYIEELLNLTKILVGVNEFKYGFLSRPLYEDWAAEAVTGFDNFINGKSNPMNTRRN
RTPFDGTSIISEGNESLQSTNSNESQISPDSTRSYEPECPVAYEKNDNPAALNLLRIA
SHKAGQNADGLPKTFRNRAFSIASTSGQLSSLSRHSTLGNADPNYLNDDDESSDDDLP
LALKMVLSHMKEKKD"
gene 243062..245470
/gene="Flc3"
/locus_tag="P301_G10811"
mRNA 243062..245470
/gene="Flc3"
/locus_tag="P301_G10811"
/product="Flc3p"
CDS 243062..245470
/gene="Flc3"
/locus_tag="P301_G10811"
/note="Similar to YGL139W 'Putative FAD transporter,
similar to Flc1p and Flc2p; localized to the ER'"
/codon_start=1
/product="Flc3p"
/protein_id="EWG90885.1"
/translation="MRFLQVYKSSALIGLIILLASKVNLAEAKRKLVATSLVTCMENS
QLSANSFDVVFNPDDRSLHYDLDMSTQIDSYIFADIDVYAYGFKIITKNVDLCSINWK
QFCPVHPGNIQIDSIEYISSEYVNEIPGIAYQVPDIDAYARVKITNNVSEYLACIQIY
FSNGKTVSQIGVKWATAVVAGIGLLLSAILSTFGNSTAASHISANTMSLFLYFQSVVV
VAMQHVHRVPPIAAAWAENLVWSMGLIRISFMQRIFRWYVQSTGGTPSLYLTSTSMSV
LAQRSWQYLMELPLIKRATNVLYGNANTLIFRGIKRLGYKMGIENTSIVCTGFTFFVL
CGYVLAGFIIVFKCCVELATRLGWIQKARFWEFRKQWRMILKGALLRYIYIGFVQLTI
LSFWEFTERDSPAVIVIACLFILLSCGLMLWAAWRTVFFARRSVALYNNPAALLYGDE
YVLHKYGFFYTMFNANHYWWNIVLLSYIFVKSLLVGFAQASGQTQVLFMFILDLFYFV
AIIYYKPYLDRPTNIMNILIATVTVVNSFLFMFFSDLFNQSYKVAAIMGWIFFIMNAA
FSFILLMMILAFAGMMLFSKNPDLRFKPAKDDRTSFQRNTMKPEGTVNRSVANELLAL
GNVAKDHDDNSDYESNDTGVNDELKQAQDETTPTTVTSSDDNKPTFSEKILSKFSRPK
NENASTDALRVEAPKQQTFPHNLTNLSRENLSTLGSKPYPGHTRSQSDAHNGLINSFE
EEDTSSNTDPFHDSTEGDLLDTSSSDGGFRSQNYVRDDSINSLGNNKQPLRKPPGFFD
EGFM"
gene complement(245840..246877)
/locus_tag="P301_G10816"
mRNA complement(245840..246877)
/locus_tag="P301_G10816"
/product="hypothetical protein"
CDS complement(245840..246877)
/locus_tag="P301_G10816"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90886.1"
/translation="MYNFLEFFFFFITYTLFKSTFVQGKSSFPGHDVCKFEDQNFQTE
FFLNVLKGDKLQNLKQEYEQYKKQSTLYTGFVIEKQYEYQVAPLQINNFLQVTFCKGG
KPIWNHILPFQKDLDWAEPLCIPAQEDDTISQNSSVCFKFARVQKYTQRNVTLYFPNK
FVGFVFVCNSSKTHPPTQNNFETIPLTPIISDDIRDYKISWSIKGRITKVVPYLHSLS
IPMSKYEMLIHSDKNISNELEYKSLTSEFWKSYKNLRKFKNKEMSWINEINPMEQYDS
SGNENVPNFHEKLDRTINRIAQNIERPHDALIRAIAAHNRNSNSGNITRRKYLRRKIS
KMLKNKIPLKN"
gene 247215..250084
/gene="Sec27"
/locus_tag="P301_G10821"
mRNA join(247215..247232,247433..250084)
/gene="Sec27"
/locus_tag="P301_G10821"
/product="Sec27p"
CDS join(247215..247232,247433..250084)
/gene="Sec27"
/locus_tag="P301_G10821"
/note="Similar to YGL137W 'Essential beta'-coat protein of
the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER
transport; contains WD40 domains that mediate cargo
selective interactions'"
/codon_start=1
/product="Sec27p"
/protein_id="EWG90887.1"
/translation="MKLDIKKTFSNRSDRVKGIDFHPTEPWVLTTLYSGRVELWNYET
QVEVRSIQVTETPVRAGKFIARKNWIIVGSDDFRIRVFNYNTGEKVVDFEAHPDYIRS
IAVHPTKPYVLSGSDDLTVKLWNWENNWALEQTFEGHEHFVMCVAFNPKDPSTFASGC
LDRTVKVWSLGQSTPNFTLTTGQERGVNYVDYYPLPDKPYMITASDDLTIKIWDYQTK
SCVATLEGHMSNVSFAVFHPTLPIIISGSEDGTLKIWNSSTYKVEKTLNVGLERSWCI
ATHPTGRKNYIASGFDNGFTVLSLGNDEPTLSLDPVGKLVWSGGKNAAASDIFTAVIR
GNEEVEQDEPLSLQTKELGSVDVFPQSLAHSPNGRFVTVVGDGEYVIYTALAWRNKAF
GKCQDFVWGPDSNSYALIDETGQIKYYKNFKEVTSWSVPMHSAIDRLFSGALLGVKSD
GFVYFFDWDNGTLVRRIDVNAKDVIWSDNGELVMIVNTNSNGDEASGYTLLFNKDAYL
EAANNGNIDDSEGVDEAFDVLYELSESITSGKWVGDVFIFTTATNRLNYFVGGKTYNL
AHYTKEMYLLGYLARDNKVYLADREVHVYGYEISLEVLEFQTLTLRGEIEEAIENVLP
NVEGKDSLTKIARFLEGQEYYEEALNISPDQDQKFELALKVGQLTLARDLLTDESAEM
KWRALGDASLQRFNFKLAVEAFTNAHDLESLFLLHSSFNNKEGLVTLAKDAERAGKFN
LAFNAYWIAGDIQGAKDLLIKSQRFSEAAFLGSTYGLGDDAVNDIVTKWKENLILNGK
NTVSERVCGAEGLPGSSSSGDAQPLIDLDSTPAPEQADENKEAEVEDSEFKESNSEAV
EAEKKEEEAPQQQQSEQQPEQGEAVPEPVEEES"
gene complement(250243..251205)
/gene="Mrm2"
/locus_tag="P301_G10826"
mRNA complement(250243..251205)
/gene="Mrm2"
/locus_tag="P301_G10826"
/product="Mrm2p"
CDS complement(250243..251205)
/gene="Mrm2"
/locus_tag="P301_G10826"
/note="Similar to YGL136C 'Mitochondrial 2' O-ribose
methyltransferase, required for methylation of U(2791) in
21S rRNA; MRM2 deletion confers thermosensitive
respiration and loss of mitochondrial DNA; has similarity
to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ'"
/codon_start=1
/product="Mrm2p"
/protein_id="EWG90888.1"
/translation="MILVYNRIRSIISSSLGRIHVRYNSNSQNRWLNRQLKDPYTKEA
KVQNLRSRAAFKLMQIDDKYRLFSKNRTDQRILDLGYAPGAWSQVARQRSSPNSMILG
VDILPCEPPHGVNSIQANILAKRTHDLIRLFFSKHFQLNRHDDLHKDHGYFQNMLEEE
LTHVKDTELYREIFTSDDIYETPNTNSTLIEREKFPVDVIISDMYEPWPQTTGFWNNI
TNQAYFRMANTSGVSIRDHYQSIDLCDAALVTAIDLLRPLGSFVCKLYTGEEENLFKK
RMQAVFTNVHKFKPDASRDESKETYYIGLKKKRNVDKLDVFSNS"
gene 251987..252640
/gene="Rpl1b"
/locus_tag="P301_G10831"
mRNA 251987..252640
/gene="Rpl1b"
/locus_tag="P301_G10831"
/product="Rpl1bp"
CDS 251987..252640
/gene="Rpl1b"
/locus_tag="P301_G10831"
/note="Similar to YGL135W 'N-terminally acetylated protein
component of the large (60S) ribosomal subunit, nearly
identical to Rpl1Ap and has similarity to E. coli L1 and
rat L10a ribosomal proteins; rpl1a rpl1b double null
mutation is lethal'"
/codon_start=1
/product="Rpl1bp"
/protein_id="EWG90889.1"
/translation="MSKITSSQVREHVKELLKYSNETKKRNFLETVELQVGLKNYDPQ
RDKRFSGSLKLPNCPRPNMSICIFGDAFDVDRAKSCGVDAMSVDDLKKLNKNKKLIKK
LSKKYNAFIASEVLIKQVPRLLGPQLSKAGKFPTPVSHNDDLYGKVTDVRSTIKFQLK
KVLCLAVAVGNVEMEEDVLVNQILMSVNFFVSLLKKNWQNVGSLVVKSSMGPAFRLY"
gene 253009..254308
/gene="Pcl10"
/locus_tag="P301_G10836"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 254632..254931
/estimated_length=300
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 255051..258845
/gene="Itc1"
/locus_tag="P301_G10841"
mRNA 255051..258845
/gene="Itc1"
/locus_tag="P301_G10841"
/product="Itc1p"
CDS 255051..258845
/gene="Itc1"
/locus_tag="P301_G10841"
/note="Similar to YGL133W 'Subunit of the ATP-dependent
Isw2p-Itc1p chromatin remodeling complex, required for
repression of a-specific genes, repression of early
meiotic genes during mitotic growth, and repression of
INO1; similar to mammalian Acf1p, the regulatory subunit
of the mammalian ATP-utilizing chromatin assembly and
modifying factor (ACF) complex'"
/codon_start=1
/product="Itc1p"
/protein_id="EWG90890.1"
/translation="MVLYKRKPILLPDPKPLPLDLNVQVWHIEETGEWFSSYEEFLER
FDFYTRHHFTCEITGTSCLTFFQALDSEETQFKYVEDRFPLKLREPVARFLHFNGIRR
LDALVEKVYARFKNDFFPGEVVYLRKQKDSSTTSSNSQQSTPQPDDMVEINSVGNPGL
PQYQYQRRYVIKEKVQFNATINPESREIVMPAHTKYMLIEEAASSNKSFIVDQGQIYR
DRSTFTKHLIKCFFKITLQRASSKMGAPWCVKPEYLAMYGLTMEWPKDMLKYKEDEPV
VARRSNSANVSSPESEKNKRQSKSSGKSNTSNDASNKKETKKKRKPTEVNDSENNSSE
EDKKKGQNVTSETHSKKRKKEANEEPNTENVESVPTPANAEPQAVTITSIMDDLALPY
QHPPNIFPNLTYYNEKLECISLGSTKLSRPFDSFGKLLQAYQFLNTFGSKICLSHFSL
DQFITSLKCTDPYELKGEVVLVNIRTQTSKEQEIENNGLPIKNKAETTTEEDSENPSD
WQRNSFIRDMIMKRNSDKVEYKIVHDDPASDDILDNINHNASALLIEVFTALLRLFIN
EEGDWSCIVVENWIIDDKGVLMERKDERGEGEAKQKRNAHGYFLQDKEKIDNLKDTLK
ENATEVQKESDAKNETNSESDSKSDSDSKERDPKLEKCLNYRNVNWIERLTKRQFNNS
YWLIILLGVLEDCRHLPMYTEFIDSFIEKIIPKDISATQLPKQLWRNFCRKLSFSDKV
NALWILVDLVSHFSPDIKAAVDDSMELCGQIRSERFKVARELKTEAAVLSNLQGDLQA
IQEKLNKTDENTPSADGADKKDDSESNSEPIDLIIIEKKQKLIEEQDKKVQALQSDKN
FLDNCLFENDLQRLKPLGLDRYGNRYFWLDHNGVPFPQYPAGMNETPKSNNSLSYHSG
RLLIQGPKASSAKFFLNVSDEQLSNWQKIRNSEGISEATREVFGISKTSSGSYNYVEN
GIEVELLDSNDRVNPLIELTPIQKKIMDETPSRLLLSPDQWYCIDKLEDLSRIMDWLD
NWGRKEHDLLRQIRPIMERIKSSLSLRDHALSLTAFTKNEEKLLKELENNEFTENELN
VDSMDVDDKNSGVKSEVDVQVDAEEKREAVIDEKLEVIADELMKLDDSSKTRNVLNRI
QELEDQRDELLEQKKSIINSQRPGARILARSERKRTKISRGNKVNKQIEILTDLVNYR
HFKAMEDVIAWKNVLANSIWGSSLRKNASGNKRSGVIETVDDKLKDIVGQTSRTVTPA
PN"
gene complement(258992..263203)
/gene="Snt2"
/locus_tag="P301_G10851"
mRNA complement(258992..263203)
/gene="Snt2"
/locus_tag="P301_G10851"
/product="Snt2p"
CDS complement(258992..263203)
/gene="Snt2"
/locus_tag="P301_G10851"
/note="Similar to YGL131C 'DNA binding protein with
similarity to the S. pombe Snt2 protein; computational
analysis suggests a role in regulation of expression of
genes encoding amine transporters'"
/codon_start=1
/product="Snt2p"
/protein_id="EWG90891.1"
/translation="MPKEEDFQLPRRREAAKNVNYNEMEIDTKLVQQIQIAEKSGAKT
KGSNSQTPRNCKRTSNPASRNEKFKYQKFLHDKNTCWNFIPTLPPSFRKNSRFSNILD
LDDAMIDLKKMSLFNTESVLLSANDTIYMISEPAGEPYYVGRVVNFVSKPEFSNTIHE
AIKTTSVFPAKFFQVRMNWFYRPRDIQEHVNTFNPRLVYASLHQDICPISSYRXXXXX
XXXXXXXDVLPNEKECIIRPNIFYFDELFDRYTLKYYKVYSTDKILNKWNSKSPFLYV
LNRRFRYIYTEPKYPLENVLKKYVFHELEVNELSPADYQWDKRCQFCKEWCIQKESLS
CDECGVCAHLYCMDPPLDRKPNKDVVWTCFSCLQKQQGTKDSHVRFLEEQALELDFIR
SVRQKIEEISSKAIKENVGYNTENCWFQYLGIYSISHIGDALNDSMFFPYPFKPSRVG
VKYQWNGCNHNVPWRRNSYISANSEEERGSTKTSELAWVLDASKITTRKLSEYIEQCK
SEICPILNVRGETCNFIDVVLKNLLFTNYDTAEAFKKCKRELSRKFLKEPSFTAVEIR
KFEEAVEKFGSELRPVCEYVGTQPMSMIVRFYYNWKKTERGLTVRGKLSKLSKNKRKK
EIANHENDVETKYIDDSSFDTEKLSLAESSFQCMFCKTDYSPMWYRVTGGSDDEKIKI
RMQTGVNEKTEISEKSPAHSKKNEKLGALCIRCARMWRRYAIKWVPPLETLRKITGTC
QNSFYSAIEGIIEENNTNKFTLSPFQAHNKLLEWELVQDSELIIRQRMKVYKNPNSFV
KMKRYSMTFHTQLYKMAVRSYRKNEFHPETMQRDLELFIEDNKEVRKAIPEQKPERAK
NTKDEFPVNIIRQSPGTIKTSDTSRNRKCNDVFIEKASNNNIPKITNASNDLIEISIK
TGGSSSGSVSVDKGFKFVKFDNKTFQRLRNSLKLVNNKLPKYNEPSTKKIKMINDIAL
SNPLNEPNGASYNYTVISHSKETSVALEKYHDGNKPSKMLEKDMILKHTKNKPKNPDT
AWANNSARTFCSVCKEKFNDNDNYEVVCGNCGLTVHYFCYAIKLPKDMKKNTNLKTFK
WLCDPCSNDLNPIISTTYQCSMCPTKDYDYDRYRSQSFKICPDALKCTSLGTWVHLVC
SLFNEDIKYGNGQSMQPALNTTAVLIKHSRFTCGVCRINGGGLVKCNKCQYRYHITCA
QNSSNFKLMFEKKNMSVDTTLPCIKDVKLNDTYTLRPILICDRHDISLEGNELYPLSY
KPQHTLSYIEQYCRYYKCESDHSLVELRYFEQLRLRHGEMPGNSHDSAIKPKIYVLPF
ERTCPHCGTNKSLYWYEDIICHSCNLRSGAQELDFDSASANISNDNGLPVEITQQLME
GIEPAMFDIDISEAGTDKNTHPSSQ"
assembly_gap 262529..262563
/estimated_length=35
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 263489..264868
/gene="Ceg1"
/locus_tag="P301_G10856"
mRNA 263489..264868
/gene="Ceg1"
/locus_tag="P301_G10856"
/product="Ceg1p"
CDS 263489..264868
/gene="Ceg1"
/locus_tag="P301_G10856"
/note="Similar to YGL130W 'Alpha (guanylyltransferase)
subunit of the mRNA capping enzyme, a heterodimer (the
other subunit is CET1, an RNA 5'-triphosphatase) involved
in adding the 5' cap to mRNA; the mammalian enzyme is a
single bifunctional polypeptide'"
/codon_start=1
/product="Ceg1p"
/protein_id="EWG90892.1"
/translation="MVLAMESRVAPEIPGLIQPGNVTQDLKMMVCKLLNSPKPTKTFP
GSQPVSFQHSDVEEKLLAHDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYL
VNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRC
LTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTFPFKISMKHMDFSYQLVKVAKSLD
KLPHLSDGLIFTPVKAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDD
RNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSD
EEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSV
SLEDLEEIVGDIKRCWDERRANMAGGSGRPLPSQSQNATLSTSKPVHSQPPSNDKEPK
YVDEDDWSD"
gene complement(265067..266533)
/gene="Rsm23"
/locus_tag="P301_G10861"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(266638..267489)
/gene="Cwc23"
/locus_tag="P301_G10866"
mRNA complement(266638..267489)
/gene="Cwc23"
/locus_tag="P301_G10866"
/product="Cwc23p"
CDS complement(266638..267489)
/gene="Cwc23"
/locus_tag="P301_G10866"
/note="Similar to YGL128C 'Component of a complex
containing Cef1p, putatively involved in pre-mRNA
splicing; has similarity to E. coli DnaJ and other
DnaJ-like proteins and to S. pombe Cwf23p'"
/codon_start=1
/product="Cwc23p"
/protein_id="EWG90893.1"
/translation="MPGHELEDVINQRLNLYDVLELPTPLDVHTIYDDLPQIKRKYRT
LALKYHPDKHPDNPSIIHKFHLLSTATNILTNADVRPHYDRWLIEFLRKTNDIERNKL
IQKLEESESSTIPTTTPHPDLLQIQRHGELLRKLKHFNLPYGDWKHLNTQDQENASQH
PYYDCSTLRIVLDNFLQSNNKSNCLSHLRNQVLITLSANEIYDIYFSERNNYSKDDSI
IIYTVFDTPITAQHVFRNWSSGNLIPTVKDISPLIPLHYYSDFNLETELNDDIARLVS
NEPILLD"
gene complement(267738..268121)
/gene="Soh1"
/locus_tag="P301_G10871"
mRNA complement(267738..268121)
/gene="Soh1"
/locus_tag="P301_G10871"
/product="Soh1p"
CDS complement(267738..268121)
/gene="Soh1"
/locus_tag="P301_G10871"
/note="Similar to YGL127C 'Subunit of the RNA polymerase
II mediator complex; associates with core polymerase
subunits to form the RNA polymerase II holoenzyme;
involved in telomere maintenance; conserved with other
metazoan MED31 subunits'"
/codon_start=1
/product="Soh1p"
/protein_id="EWG90894.1"
/translation="MSSTNGNAPATPSSDQNPLPTRFEVELEFIQSLANIQYVTYLLT
QXXIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLFILKLLNGFMESAIVNEDGLLEG
LDELPKIIQLQGPQWMNEMVERWAN"
gene 268343..269485
/gene="Scs3"
/locus_tag="P301_G10876"
mRNA 268343..269485
/gene="Scs3"
/locus_tag="P301_G10876"
/product="Scs3p"
CDS 268343..269485
/gene="Scs3"
/locus_tag="P301_G10876"
/note="Similar to YGL126W 'Protein required for inositol
prototrophy, identified as an ortholog of the FIT family
of proteins involved in triglyceride droplet biosynthesis;
disputed role in the synthesis of inositol phospholipids
from inositol'"
/codon_start=1
/product="Scs3p"
/protein_id="EWG90895.1"
/translation="MSSKWFNAIHLLVCPLTVLVGYLMNAYGYGAALQATLNKDGLVN
AMLVKKGWFWTSLVGWWCIIRYRAVPGATGRDRRHIVQSFKRYAILTVWWYVFTQGIW
FGVGPIMDLVFVYTGGHCHYDVFDDAGHVNEDFQGSVTRTNRALALIHNVLTLHGHHQ
EHRQQQLWDRSIGSIQGALQATQPKTPKNVTASAAAAINTFIHDQMHRWQGPLTTSAQ
CRRFGGHWAGGHDPSGHVFLATLMCMFLLGELRVFGRRALAHLYAQKWQLVRLVTCLF
DTGPLWTWRRCGGGSMTCGARLWRAIVEPPVTCAAALLRLTRCIACDHPVIILLTLLV
TWLWQLLLTAVASRFHTVREHMSGLLAAYIVTGLVYARDAAALRPV"
gene 269859..271661
/gene="Met13"
/locus_tag="P301_G10891"
mRNA 269859..271661
/gene="Met13"
/locus_tag="P301_G10891"
/product="Met13p"
CDS 269859..271661
/gene="Met13"
/locus_tag="P301_G10891"
/note="Similar to YGL125W Major isozyme of
methylenetetrahydrofolate reductase, catalyzes the
reduction of 5,10-methylenetetrahydrofolate to
5-methyltetrahydrofolate in the methionine biosynthesis
pathway"
/codon_start=1
/product="Met13p"
/protein_id="EWG90896.1"
/translation="MKITEKLEQHRQTSGKPTYSFEYFVPKTTQGVQNLYDRMDRMYE
ASLPQFIDITWNAGGGRLSHLSTDLVATAQSVLGLETCMHLTCTNMPISMIDDALENA
YHSGCQNILALRGDPPRDAENWTPVEGGFQYAKDLIKYIKSKYGDHFAIGVAGYPECH
PELPNKDVKLDLEYLKQKIDAGGDFIITQMFYDVDNFINWCSQVRAAGMDVPIIPGIM
PITTYAAFLRRAQWGQISIPQHFSSRLDPIKDDDELVRDIGTNLIVEMCQKLLDSGYV
SHLHIYTMNLEKAPLMILERLNILPMESEFNAHPLAVLPWRKSLNPKRKNEEVRPIFW
KRRPYSYVARTSQWAVDEFPNGRFGDSSSPAFGDLDLCGSDLIRQSANKCLELWSTPT
SINDVAFLVINYLNGNLKCLPWSDIPINDEINPIKAHLIELNQHSIITINSQPQVNGI
RSNDKIHGWGPKDGYVYQKQYLEFMLPKTKLPKLIDTLKNNEFLTYFAIDSQGDLLSN
HPDNSKSNAVTWGIFPGREILQPTIVEKISFLAWKEEFYHILNEWKLNMNKYDKPHSA
QFIQSLIDDYCLVNIVDNDYISPDDQIHSILLSL"
assembly_gap 271927..272032
/estimated_length=106
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(272119..274054)
/gene="Mon1"
/locus_tag="P301_G10901"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 274958..275722
/gene="Rps2"
/locus_tag="P301_G10911"
mRNA 274958..275722
/gene="Rps2"
/locus_tag="P301_G10911"
/product="Rps2p"
CDS 274958..275722
/gene="Rps2"
/locus_tag="P301_G10911"
/note="Similar to YGL123W 'Protein component of the small
(40S) subunit, essential for control of translational
accuracy; phosphorylation by C-terminal domain kinase I
(CTDK-I) enhances translational accuracy; methylated on
one or more arginine residues by Hmt1p'"
/codon_start=1
/product="Rps2p"
/protein_id="EWG90897.1"
/translation="MSAPEAQQQKRGGFGGRNRGRPNRRGPRNTEEKGWVPVTKLGRL
VKAGKITTIEEIFLHSLPVKEFQIIDTLLPGLQDEVMNIKPVQKQTRAGQRTRFKAVV
VVGDSNGHVGLGIKTAKEVAGAIRAGIIIAKLSVIPIRRGYWGTNLGQPHSLATKTTG
KCGSVTVRLIPAPRGSGIVASPAVKKLLQLAGVEDVYTQSNGKTRTLENTLKAAFVAI
GNTYGFLTPNLWAEQPLPVSPLDIYSDEASAQKKRF"
gene complement(276204..277781)
/gene="Nab2"
/locus_tag="P301_G10921"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(278035..278415)
/gene="Gpg1"
/locus_tag="P301_G10926"
mRNA complement(278035..278415)
/gene="Gpg1"
/locus_tag="P301_G10926"
/product="Gpg1p"
CDS complement(278035..278415)
/gene="Gpg1"
/locus_tag="P301_G10926"
/note="Similar to YGL121C 'Proposed gamma subunit of the
heterotrimeric G protein that interacts with the receptor
Gpr1p; involved in regulation of pseudohyphal growth;
requires Gpb1p or Gpb2p to interact with Gpa2p;
overproduction causes prion curing'"
/codon_start=1
/product="Gpg1p"
/protein_id="EWG90898.1"
/translation="MFYLSDIEEEASAGAEPTYNFWEVLLFSNTQENLVTVVGELHTL
TDRVVHYKIEPESREVTATTLPSLLALLLEKRNQARRLYRDVLSMKMSELDWDIDDLF
TQLQEELTRTDDTLSMYPRRRFYH"
gene complement(278889..281192)
/gene="Prp43"
/locus_tag="P301_G10931"
mRNA complement(278889..281192)
/gene="Prp43"
/locus_tag="P301_G10931"
/product="Prp43p"
CDS complement(278889..281192)
/gene="Prp43"
/locus_tag="P301_G10931"
/note="Similar to YGL120C RNA helicase in the DEAH-box
family, functions in both RNA polymerase I and polymerase
II transcript metabolism, involved in release of the
lariat-intron from the spliceosome"
/codon_start=1
/product="Prp43p"
/protein_id="EWG90899.1"
/translation="MGSKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVH
HDAGEFKGLQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEF
LKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRV
AEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAH
ERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVEL
YYTPEFQRDYLDSAIRTVLQIHATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREE
GCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVV
DPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELI
EQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEG
NLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRAD
DAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLE
RLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIH
PSTVLGHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSL
ERIKEKVDRLNELKQGKNKKKSKHSKK"
gene 281697..283202
/gene="Abc1"
/locus_tag="P301_G10941"
mRNA 281697..283202
/gene="Abc1"
/locus_tag="P301_G10941"
/product="Abc1p"
CDS 281697..283202
/gene="Abc1"
/locus_tag="P301_G10941"
/note="Similar to YGL119W 'Protein required for ubiquinone
(coenzyme Q) biosynthesis and for respiratory growth;
exhibits genetic interaction with COQ9, suggesting a
common function; similar to prokaryotic proteins involved
in early steps of ubiquinone biosynthesis'"
/codon_start=1
/product="Abc1p"
/protein_id="EWG90900.1"
/translation="MVTNMVKLRNLRRLYCSSRLLRTIQNGRISSVSSISLSKKYTTK
SAKEGEENVERKHEEEKKDTLKSSSVPTSRISRLFHYGSLAAGVGMNAAAKGISEVAK
GNSPTWKSLILSDSNIDRITNKFSKMRGVALKIGQLLSFQDEKVLPKELYEILSRVQN
SANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQ
YPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKF
EALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCL
EEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDR
KGAYEMSVQLGYLTGLESQSMKDAHVDSVLTLGEPFRGDVDKSFDFKDQTVSDRIRGN
IGLMLNERLCPPPEETYSLHRKFSGIFLLCARMGASVHCAKLFKEIFAYKV"
assembly_gap 283346..283795
/estimated_length=450
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(284602..284707)
/locus_tag="P301_G10951"
tRNA complement(284602..284707)
/locus_tag="P301_G10951"
/product="tRNA-Trp"
/note="TW(CCA)G1"
gene 285763..286560
/locus_tag="P301_G10961"
mRNA 285763..286560
/locus_tag="P301_G10961"
/product="hypothetical protein"
CDS 285763..286560
/locus_tag="P301_G10961"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90901.1"
/translation="MQPISIKDVESDQGKVYIVNALKDLVCKCLLEFVDIQIESFMYP
DDPKCFTRIFKGNKIVNEASDKDSKVRSYPSSLGVGHSALFPLIYIRQKTNSLRFLND
PKQLPTPLVDDMNAKFKGIIKVYENLIHLYHSYQTVDCNNMNQQKLLGDLVSRGNFML
DILHGYVTIASTIVRDSKDANILIDTVNRFIHDTILFHKRIIHNSNAYTEYLVMKRGM
QRNQSEETLVELEFRTLDVSDVNLDNEFDDFLQHRKTSLKITHRRVI"
gene 287060..288892
/gene="Cdc20"
/locus_tag="P301_G10966"
mRNA 287060..288892
/gene="Cdc20"
/locus_tag="P301_G10966"
/product="Cdc20p"
CDS 287060..288892
/gene="Cdc20"
/locus_tag="P301_G10966"
/note="Similar to YGL116W 'Cell-cycle regulated activator
of anaphase-promoting complex/cyclosome (APC/C), which is
required for metaphase/anaphase transition; directs
ubiquitination of mitotic cyclins, Pds1p, and other
anaphase inhibitors; potential Cdc28p substrate'"
/codon_start=1
/product="Cdc20p"
/protein_id="EWG90902.1"
/translation="MPESSRDKGNAAISGNRSVLSIASPTKLNILSSDWSRNQGKVSK
NSLKRSSSLNIRNSKRPSLQASANSIYSRPKITIGAPPLIRRDSSFFKDEFDAKKDKA
TFSAYSSRSYPTIGSDSVVSQTSLSQPTTSREVDEQFTVAADRYIPILQGASQNKVDP
ETLHEALPPPNASPISHLRAQTKIVFKQNVAEACGLDMNKRILQYMPEPPKCSSLRQK
SYIMKKRTHYSYQQEQKIPDLIKLRKINTNPERILDAPGFQDDFYLNLLSWSKKNVLA
IALDTALYLWNATTGDVSLLTDFENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMS
LIRTMRSGLGVRIGSLSWLDTLIATGSRSGEIQINDVRIKQHIVSTWAEHTGEVCGLS
YKSDGLQLASGGNDNTVMIWDTRTSLPQFSKKTHSAAVKALSWCPYSPNILASGGGQT
DKHIHFWNSITGARVGSINTGSQVSSLHWGQSHTSTNGGMMNKEIVATGGNPENAISV
YNYETKFKVAEVVHAHEARICCSQLSPDGTTLATVGGDENLKFYKIFDPRCTGRSRED
GLMDGMLGLIGKEGCRTNDKENRSKNSSEIHTRRPSSTSQYLIR"
gene 289283..290251
/gene="Snf4"
/locus_tag="P301_G10971"
mRNA 289283..290251
/gene="Snf4"
/locus_tag="P301_G10971"
/product="Snf4p"
CDS 289283..290251
/gene="Snf4"
/locus_tag="P301_G10971"
/note="Similar to YGL115W 'Activating gamma subunit of the
AMP-activated Snf1p kinase complex (contains Snf1p and a
Sip1p/Sip2p/Gal83p family member); activates
glucose-repressed genes, represses glucose-induced genes;
role in sporulation, and peroxisome biogenesis'"
/codon_start=1
/product="Snf4p"
/protein_id="EWG90903.1"
/translation="MKPTQDSQEKVSIEQQLAVESIRKFLNSKTSYDVLPVSYRLIVL
DTSLLVKKSLNVLLQNSIVSAPLWDSKTSRFAGLLTTTDFINVIQYYFSNPDKFELVD
KLQLDGLKDIERALGVDQLDTASIHPSRPLFEACLKMLESRSGRIPLIDQDEETHREI
VVSVLTQYRILKFVALNCRETHFLKIPIGDLNIITQDNMKSCQMTTPVIDVIQMLTQG
RVSSVPIIDENGYLINVYEAYDVLGLIKGGIYNDLSLSVGEALMRRSDDFEGVYTCTK
NDKLSTIMDNIRKARVHRFFVVDDVGRLVGVLTLSDILKYILLGSN"
gene 290710..292887
/locus_tag="P301_G10976"
mRNA 290710..292887
/locus_tag="P301_G10976"
/product="hypothetical protein"
CDS 290710..292887
/locus_tag="P301_G10976"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90904.1"
/translation="MPQSTPSQEVQRVPWDNKPALKQITLRATIAGIAIGSLVLTSNF
QFGLQTGWVSMMSLPSALLACAFFKNIWPLIFPNDRPFSDVENVYVQSMAVAVGTGPL
AFGFVGVIPAIEKFLTNDESGGLREQGQSFTFRELLIWSTALAFFGIFFAVPLRKQVI
VREKLPFPSGSATATLISVLNGTEILQEVSKSELLEMRQRRLNECPEVLQPNRDPEET
DYLMNSSHSELGDYTATSQDGSSILSTGSENYRANIIILLKTFVVSSLYTMVSYFVPV
IRSIPVFGKYLSNNYLWNFQPSPAYIGQGIIMGLPTVSYMLIGCFLGWGVLAPLARYK
GWVPPDADVHDWEEGVQGWILWSSLSIMVADSVVAFIVVTVKSIVKFILIDDKAALLN
NIIDDTFQSMLLEEERAINSSRRNTYVDGRQDTVRLVSRDNEIEVDSKHLVRYTTVIS
GCLVSSIICIVSIIYLFGIQVIPLYAIITALILALFLSILGIRALGETDLNPVSGIGK
ISQLIFAFIIPRDRPGSVLMNVVSGGIAEASAQQAGDLMQDLKTGHLLGASPRAQFCA
QLIGACWSIILSSFMYLCYNKVYSIPSEQFRIPTAVVWIDCARLVTGKGLPDKALECS
MILGVIFAVLSLIRNTYRDYGYGWILYIPSGVAVGVGIFNSPSFTIARFIGGWASHFW
LKNHRGDLNAKTKMIVFSSGLVLGEGIFSVINMVFICLNVPHY"
gene 293182..295188
/gene="Sld3"
/locus_tag="P301_G10986"
mRNA 293182..295188
/gene="Sld3"
/locus_tag="P301_G10986"
/product="Sld3p"
CDS 293182..295188
/gene="Sld3"
/locus_tag="P301_G10986"
/note="Similar to YGL113W 'Protein involved in the
initiation of DNA replication, required for proper
assembly of replication proteins at the origins of
replication; interacts with Cdc45p'"
/codon_start=1
/product="Sld3p"
/protein_id="EWG90905.1"
/translation="METWEVIASVKEATKGLDLSLDHPLIIKSEDVPSNILQLLQQKN
RRQLKHICMKSRKEYFLLEEYGPGFWVKWPYNYFNGYSLPERRTEVVTTVERERAKRE
TLKTWDELKFKELLHLWSEEPKGSCKLEKDKDLKLDMNPPDMKGESKINDYYSDPKEY
IESKYYDALFSIHTPLAYFVKSNLVRLKNTCRTKYGSDSYKIAYQAMLQKFLLSIVQF
KDRHDNRLLLEPFSSPIADEKRKNCLTKFVIQDENKNSSTIADLCVVLKSREIKLQIL
LLLEIIGLNDLDWNFRDFEKKYKLKLKKRSLNLTKKGLVRRRSKKKTSEKDKGIERIT
TSLDYCEQLDLYLDRACILDILLSSETPNPDAIEASNGTIQEHKKNILDKSKEASLVG
FINYVLIPYFNKKVPHAVEFIIQKLKGPSMRPKRALKKVNDSTNVSSPNTVETYNRLS
TSQRASRSSIINSVPSSPALRRVDANLFSRKSIASPTPELLNSRTNSNLNEFLESETR
SLKRPSQLGRTKSDLTMNHLQKRQFSVSDLSTTRVPNSSTITLKTPFSHSTINAYKTM
NNSFRRVGKRKDINETIRLHERVDSEENVQVQATPAVKKRTVTPNKKAQLQSIIESPL
NFKDDDTHEGRKNTSNITSTPTNKPPENSSKRRVRRRLFAPEST"
gene complement(295424..296974)
/gene="Taf6"
/locus_tag="P301_G10996"
mRNA complement(295424..296974)
/gene="Taf6"
/locus_tag="P301_G10996"
/product="Taf6p"
CDS complement(295424..296974)
/gene="Taf6"
/locus_tag="P301_G10996"
/note="Similar to YGL112C Subunit (60 kDa) of TFIID and
SAGA complexes, involved in transcription initiation of
RNA polymerase II and in chromatin modification, similar
to histone H4"
/codon_start=1
/product="Taf6p"
/protein_id="EWG90906.1"
/translation="MSTQQQSYTIWSPQDTVKDVAGSLGLENINDDVLKALAMDVEYR
ILEIIEQAVKFKRHSKRDVLTTDDVSKALRVLNVEPLYGYYDGSEVNKAVSFSKVNTS
GGQSVYYLDEEEVDFDRLINEPLPQVPRLPTFTTHWLAVEGVQPAIIQNPNLNDIRVS
QPPFIRGAIVTALNDNSLQTPVTSTTASASVTDTGASQHLSNVKPGQNTEVKPLVKHV
LSKELQIYFNKVISTLTAKSQADEAAQHMKQAALTSLRTDSGLHQLVPYFIQFIAEQI
TQNLSDLQLLTTILEMIYSLLSNTSIFLDPYIHSLMPSILTLLLAKKLGGSPKDDSPQ
EIHEFLERTNALRDFAASLLDYVLKKFPQAYKSLKPRVTRTLLKTFLDINRVFGTYYG
CLKGVSVLEGESIRFFLGNLNNWARLVFNESGITLDNIEEHLNDDSNPTRTKFTKEET
QILVDTVISALLVLKKDLPDLYEGKGEKVTDEDKEKLLERCGVTIGFHILKRDDAKEL
ISAIFFGE"
gene 297224..298615
/gene="Nsa1"
/locus_tag="P301_G11001"
mRNA 297224..298615
/gene="Nsa1"
/locus_tag="P301_G11001"
/product="Nsa1p"
CDS 297224..298615
/gene="Nsa1"
/locus_tag="P301_G11001"
/note="Similar to YGL111W Constituent of 66S pre-ribosomal
particles, involved in 60S ribosomal subunit biogenesis"
/codon_start=1
/product="Nsa1p"
/protein_id="EWG90907.1"
/translation="MRLLVSCVDSGSIKEVLCNIGTDTSVQSALQPFHVAPHLAEGLK
AYVDRMWVISEDEAILARNSGVVELVKISKHLKENEALQVDPKGESKNEKSLSDDLPK
FDISEFEITSSVSDLFDDAKLESLSSKSVKRTKLVDGFVTLCPIKKDSSNNTFVAATK
SGLLHIIKKGEDKKLIKLASLGLKAPVEFLQLYDLEDTDTDKYIFAYGGEENLIKLVE
IDSSFQSLKQIWEAKNVKNDRLDMRVPVWPMALRFLEPSPGKTEKGKLNYQFAAITRW
SHLTKYSTQHGRKPFAQIDLLPNREPLSQMEVFDAKGENVVSSLGNFQSETFNELNVI
TTDYKKNVFKFDGNGRMLGKVGRDDITGSSTYIHVHDGKYLLQGGLDRYVRIFDIKTN
KMLVKVYVGSRINFIVMLDDVEIEMPLSPSAKAAKEKQKRKVTELEEDADELWNKLEG
KVAASKASKKSKI"
gene complement(298783..300657)
/gene="Cue3"
/locus_tag="P301_G11006"
mRNA complement(298783..300657)
/gene="Cue3"
/locus_tag="P301_G11006"
/product="Cue3p"
CDS complement(298783..300657)
/gene="Cue3"
/locus_tag="P301_G11006"
/note="Similar to YGL110C 'Protein of unknown function;
has a CUE domain that binds ubiquitin, which may
facilitate intramolecular monoubiquitination'"
/codon_start=1
/product="Cue3p"
/protein_id="EWG90908.1"
/translation="MLSRYNRVIEINGGNADISLPIVKFPPFKLRAQLIEKDPVVWLH
LIETYVAYFEYLMQGANVELLDESTLDHLRLFLRTYLHEIADEEGKLLSLGINHDVSE
QLYLLKGWIFSLIKKCGLLHLQIFGDSLWNLIKVYVRRNPDSIRGLIDGSLKPRINTQ
RVQLDKSYQVQQHLKQLIESGKFKRIDLRCVEDLLSAKSMQPNKFAENFFTANWIEIL
EALWAKGQGRGHKEARELIIISLFSVSADRLLKITKELGISNFETLALYPLLGTMLIN
EGVHKRLPDLKSKLLFLNLGGLSMDEGDHMSYPISSGTEVNEEQLSALMELFPQFSKY
QLSQTLLAYDNNIELVTNKIFEDPTIIEAFPREPAEEEVEPVSDGDNASFTEELSILD
RGDSSKNKELDKKIISEGVPDELRNKTLTRALKLLYEADEDERDDTYDEADVNRSDPS
KRIGLQEDEESYDTKDDSNEVRQDHNYHIVEAYLWNLLKEDPKLFERSKRGTKVRKTM
KEMTSWSDEKIEGWCRMLERSPTRARLLEKKFMFKGNSKTGKTSYVHNRDSQNDGNVV
KEQAKQKKSENIKKHEPQSTEQKKRQHAKNEKRKGARANHNRKKGHDKKLARAGNNAI
"
gene complement(300894..301316)
/locus_tag="P301_G11016"
mRNA complement(300894..301316)
/locus_tag="P301_G11016"
/product="hypothetical protein"
CDS complement(300894..301316)
/locus_tag="P301_G11016"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90909.1"
/translation="MGLCGSKTQPMPSQTTTVATKARTKPINRDTVKSKQELRHKEKK
DKKKKTQLKSTTVPVVQKKEGSKLTDTSDPSKNKVSPKEAARLAAEKRFQETNEKYNK
GELGKKLAQERAKSHKTRLMEEAEKKHAERERENMIYD"
gene complement(301577..303517)
/gene="Rmd9"
/locus_tag="P301_G11021"
mRNA complement(301577..303517)
/gene="Rmd9"
/locus_tag="P301_G11021"
/product="Rmd9p"
CDS complement(301577..303517)
/gene="Rmd9"
/locus_tag="P301_G11021"
/note="Similar to YGL107C 'Mitochondrial protein required
for respiratory growth; mutant phenotype and genetic
interactions suggest a role in delivering mt mRNAs to
ribosomes; located on matrix face of the inner membrane
and loosely associated with mitoribosomes'"
/codon_start=1
/product="Rmd9p"
/protein_id="EWG90910.1"
/translation="MMLRRNAVRSLKTMEISVSNVVNSGSIAMLRGKLANVVLSDRTY
HSSPIFHKNVPKGVLDKKNGREQRKTEQNVSNVDPASPWRHELLSFDECVSSALKYST
TPLQNTYKRIGNNELNKNPSFAMFWDSMGRAMELYYSLRESPDFNAYRVSRLIHLLHN
GLRSTRDQLVKLSRKPDYDSQSFHKEMMNFLCNSLKDISDDILIGKVSVSGYGATHLL
TSFKELSFDDDCIRIWEASKNLSDETTSQAFQEPKVVGFMLPLLYAKTRSLTEPNELY
NQIIQSKEFIHPNLYSGLIKVFIKAEDYEKALSLFGQLCEKAEVRNYGYLIETHLSFI
GDSKNLTLAESFFDKIINDEMPYKIILQVSTVNSFLQNIWKAQNDFDHVYRIWEKAVK
FYGNTVNPGILSSLNNTFFTIFFENYVNDNINGFRKLQXIITFYSGVKKIDEPFFNVM
LTRASIWHERSIIDFIDKNYTLYHIPRTIISYRILLKSLGSIDNTNNEEILDRWLELV
KKLNELGQQYIANADLSALRDATVVWSQSKRDEKVFSAKAKGTPATTTITEDDIKVPK
PLEDLKNEDSTSNSEDRIELYLKILKRYTPYFRATKQVYRYTTGCAESYPILNEYLSG
YSDLSAEDIPVPQLHSFIAKEQ"
gene 303804..304253
/gene="Mlc1"
/locus_tag="P301_G11026"
mRNA 303804..304253
/gene="Mlc1"
/locus_tag="P301_G11026"
/product="Mlc1p"
CDS 303804..304253
/gene="Mlc1"
/locus_tag="P301_G11026"
/note="Similar to YGL106W 'Essential light chain for
Myo1p, light chain for Myo2p; stabilizes Myo2p by binding
to the neck region; interacts with Myo1p, Iqg1p, and Myo2p
to coordinate formation and contraction of the actomyosin
ring with targeted membrane deposition'"
/codon_start=1
/product="Mlc1p"
/protein_id="EWG90911.1"
/translation="MSATRANKDIFTLFDKKGQGAIAKDSLGDYLRAIGYNPTNQLVQ
DIINADSSLRDASSLTLDQITGLIEVNEKELDATTKAKTEDFVKAFQVFDKESTGKVS
VGDLRYMLTGLGEKLTDAEVDELLKGVEVDSNGEIDYKKFIEDVLRQ"
assembly_gap 304500..304614
/estimated_length=115
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 304715..305845
/gene="Arc1"
/locus_tag="P301_G11031"
mRNA 304715..305845
/gene="Arc1"
/locus_tag="P301_G11031"
/product="Arc1p"
CDS 304715..305845
/gene="Arc1"
/locus_tag="P301_G11031"
/note="Similar to YGL105W 'Protein that binds tRNA and
methionyl- and glutamyl-tRNA synthetases (Mes1p and
Gus1p), delivering tRNA to them, stimulating catalysis,
and ensuring their localization to the cytoplasm; also
binds quadruplex nucleic acids'"
/codon_start=1
/product="Arc1p"
/protein_id="EWG90912.1"
/translation="MSDLVTKFESLIISKYPVSFTKEQSAQAAQWESVLKSGQIQPHL
DQLNLVLRDNTFIVSTLYPTSTDVHVFEVALPLIKDLVASSKDVKSTYTTYRHILRWI
DYMQNLLEVSSTDKLEINHDLDLPHEVIEKKKKAPAGGAADAAAKADEDVSKKAKKQD
HPRGKPDEETLKKLREEAKAKKAAKKAANAKQQQEQQNKAPEKPKPSAIDFRVGFIQK
AIKHPDADSLYVSTIDVGDEEGPRTVCSGLVKHFPLDAMQERYVVVVCNLKPVNMRGI
KSTAMVLCGSNDDKVEFVEPPKDSKAGDKVFFEGFGDEAPMKQLNPKKKIWEHLQPHF
TTNDGLEVIFKDEEEKDHPVRKLTNAKGESFKVASIANAQVR"
gene complement(305991..307451)
/gene="Vps73"
/locus_tag="P301_G11036"
mRNA complement(305991..307451)
/gene="Vps73"
/locus_tag="P301_G11036"
/product="Vps73p"
CDS complement(305991..307451)
/gene="Vps73"
/locus_tag="P301_G11036"
/note="Similar to YGL104C 'Mitochondrial protein; mutation
affects vacuolar protein sorting; putative transporter;
member of the sugar porter family'"
/codon_start=1
/product="Vps73p"
/protein_id="EWG90913.1"
/translation="MNRILSSASLLSNVSMPRQNKHKITKALCYAIIVASIGSIQFGY
HLSELNAPQQVLSCSEFDIPMEGYPYDRTWLGKRGYKQCIPLNDEQIGIVTSVFCIGG
ILGSYFATSLANIYGRKFSSLINCTLNIVGSLIIFNSNSYRGLIIGRILVGISCGSLI
VIIPLFIKEVAPSGWEGLLGSMTQICIRLGVLLTQGIALPLTDSYRWRWILFGSFLIA
VLNFFMWFIVDESPKWLLAHGRVTDAKLSLCKLRGVTFDEAAQEIQDWQLQIESGDPL
IEPTTTNSISGYNSLWKYLRDRTNVKSRHVITVLLFGQQFCGINSIVLYGTKIISQLY
PQHAIRINFFISMVNVLVTILVSLLIHSLPRKPLLMTSTVLVSVTAFIMGIAMNHNKM
NLLIVFSFIYMGVFTMGLNPLPFIIMREVSKPQDMVLAQRYGTICNWVGTFIIAYTFP
IIHDVLSGYVFIIFAIIACSISAFIWKKVPETKRSG"
gene 308245..309206
/gene="Rpl28"
/locus_tag="P301_G11046"
mRNA join(308245..308293,308806..309206)
/gene="Rpl28"
/locus_tag="P301_G11046"
/product="Rpl28p"
CDS join(308245..308293,308806..309206)
/gene="Rpl28"
/locus_tag="P301_G11046"
/note="Similar to YGL103W 'Ribosomal protein of the large
(60S) ribosomal subunit, has similarity to E. coli L15 and
rat L27a ribosomal proteins; may have peptidyl transferase
activity; can mutate to cycloheximide resistance'"
/codon_start=1
/product="Rpl28p"
/protein_id="EWG90914.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
gene 309472..310119
/locus_tag="P301_G11056"
mRNA 309472..310119
/locus_tag="P301_G11056"
/product="hypothetical protein"
CDS 309472..310119
/locus_tag="P301_G11056"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90915.1"
/translation="MTAVNIWKPEDNIPREILAILSKPHPNYQLAFLNIIQLLKTQRR
TGWVDHGIDPCESISDHMYRMGLTTMLITDKNVDRNKCIRIALVHDFAESLVGDITPN
DPMTKEEKHRREFETVKYLCESIIRPCSESASREILDDWLAYEKQTCLEGRYVKDIDK
YEMLVQCFEYEQKYNGKKDLKQFWGAINDIKTDEVKKWTQSLLEDRQAFFDSLKE"
gene 310513..311562
/gene="Seh1"
/locus_tag="P301_G11061"
mRNA 310513..311562
/gene="Seh1"
/locus_tag="P301_G11061"
/product="Seh1p"
CDS 310513..311562
/gene="Seh1"
/locus_tag="P301_G11061"
/note="Similar to YGL100W 'Nuclear pore protein of the
conserved Nup84p complex (Nup84p, Nup85p, Nup120p,
Nup145p, and Seh1p); part of the SEA (Seh1-associated)
complex, a coatamer-related complex that associates
dynamically with the vacuole; homologous to Sec13p'"
/codon_start=1
/product="Seh1p"
/protein_id="EWG90916.1"
/translation="MQPFDSGHDDLVHDVVYDFYGRHVATCSSDQHIKVFKLDKDTSN
WELSDSWRAHDSSIVAIDWASPEYGRIIASASYDKTVKLWEEDPDQEECSGRRWNKLC
TLNDSKGSLYSVKFAPAHLGLKLACLGNDGILRLYDALEPSDLRSWTLTSEMKVLSIP
PANHLQSDFCLSWCPSRFSPEKLAVSALEQAIIYQRGKDGKLHVAAKLPGHKSLIRSI
SWAPSIGRWYQLIATGCKDGRIRIFKITEKLSPLASEESLTNSNMFDNGADVDMDAQG
RSDSNTEEKAELQSNLQVELLSEHDDHNGEVWSVSWNLTGTILSSAGDDGKVRLWKAT
YSNEFKCMSVITAQQ"
gene 311910..313832
/gene="Lsg1"
/locus_tag="P301_G11066"
mRNA 311910..313832
/gene="Lsg1"
/locus_tag="P301_G11066"
/product="Lsg1p"
CDS 311910..313832
/gene="Lsg1"
/locus_tag="P301_G11066"
/note="Similar to YGL099W 'Putative GTPase involved in 60S
ribosomal subunit biogenesis; required for the release of
Nmd3p from 60S subunits in the cytoplasm'"
/codon_start=1
/product="Lsg1p"
/protein_id="EWG90917.1"
/translation="MPPKEAPKKWKAPKGPKPTHRKNKNKLELGRAIKYARQKENAIE
YLPDGEMRFTTDKHEANWVKLRSVTQESALDEFLSTAALADKDFTADRHSNVKIIRMD
SGNDSATSQGFSMTNEQRGNLNAKQRALTKDLIVPRRPEWNEGMSKFQLDRQEKEAFL
EWRRKLAHLQESNEDLLLTPFERNIEVWKQLWRVVERSDLVVQIVDARNPLLFRSVDL
ERYVKESDNRKANLLLVNKADLLTKKQRIAWAKYFISKNISFTFYSALRANQLLEKQK
EMGEDYREQDFEEADEEGFDADEKVMEKVKILSIDQLEELFLSKAPNEPLLPPLPGQP
PLINIGLVGYPNVGKSSTINSLVGAKKVSVSSTPGKTKHFQTIKLSDSVMLCDCPGLV
FPNFAYNKGELVCNGVLPIDQLRDYIGPAGLVAERIPKYYIEAIYGIHIQTKSRDEGG
NGDIPTAQELLVAYARARGYMTQGYGSADEPRASRYILKDYVNGKLLYVNPPPHLEDD
TPYTREECEEFNKDLYVFDRLPDTRKEQVQNAAKAKGIDIVDLARDLNQLTFSAHTGG
DTQKEAKSVTHGGKQAALYNAAEDLDRDFFKMNNVEGRLSTPFHKVQNSSAGKRHNKK
NKSKNAKSKVFSIENN"
gene 314067..314334
/locus_tag="P301_G11071"
ncRNA 314067..314334
/ncRNA_class="snoRNA"
/locus_tag="P301_G11071"
/product="SNR82"
gene 314621..315358
/gene="Use1"
/locus_tag="P301_G11076"
mRNA 314621..315358
/gene="Use1"
/locus_tag="P301_G11076"
/product="Use1p"
CDS 314621..315358
/gene="Use1"
/locus_tag="P301_G11076"
/note="Similar to YGL098W 'Essential SNARE protein
localized to the ER, involved in retrograde traffic from
the Golgi to the ER; forms a complex with the SNAREs
Sec22p, Sec20p and Ufe1p'"
/codon_start=1
/product="Use1p"
/protein_id="EWG90918.1"
/translation="MAETSNDPFLSYVLSSKQLTNLNRLRRKAVTKQLGSSDDNKVSE
EFLRYQHTYQREAFEYLQTKHDAHKIMESQYEQYQSSSKTRRYSIDLDSVDAVDTESQ
TEYPNEEFIDRNEDSEAVMELRKRLLGKGQNKGLGYETTKSVDRQIEDQDTLQQDLIQ
DMSKLVGSLKQGAVAFQSALDEDKQVLGAAEIGIQVASQGLMDVSGKLRKYDKSKLSY
LFYITVFIFMILGLVFTFIIIQLFPAL"
assembly_gap 315875..317104
/estimated_length=1230
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 319023..320471
/gene="Srm1"
/locus_tag="P301_G11081"
mRNA 319023..320471
/gene="Srm1"
/locus_tag="P301_G11081"
/product="Srm1p"
CDS 319023..320471
/gene="Srm1"
/locus_tag="P301_G11081"
/note="Similar to YGL097W 'Nucleotide exchange factor for
Gsp1p, localizes to the nucleus, required for
nucleocytoplasmic trafficking of macromolecules;
suppressor of the pheromone response pathway; potentially
phosphorylated by Cdc28p'"
/codon_start=1
/product="Srm1p"
/protein_id="EWG90919.1"
/translation="MVKRTVATNGDASGAHRAKKMSKTHASHIINAQEDYKHMYLSVQ
PLDIFCWGTGSMCELGLGPQAKNKEVKRPRLNPFLPRDEAKIISFAVGGMHTLALDEE
SNVWSWGCNDVGALGRDTSNAKEQLKDMDADDSSDDEDGDLNELESTPAKIPRESFPP
LAEGHKVVQLAATDNMSCALFSNGEVYAWGTFRCNEGILGFYQDKIKIQKTPWKVPTF
SKYNIVQLAPGKDHILFLDEEGMVFAWGNGQQNQLGRKVMERFRLKTLDPRPFGLRHV
KYIASGENHCFALTKDNKLVSWGLNQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRS
IAAGEHHSLILSQDGDLYSCGRLDMFEVGIPKDNLPEYTYKDVHGKARAVPLPTKLNN
VPKFKSVAAGSHHSVAVAQNGIAYSWGFGETYAVGLGPFEDDTEVPTRIKNTATQDHN
IILVGCGGQFSVSGGVKLSDEDAEKRADEMDD"
assembly_gap 320637..321479
/estimated_length=843
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 322778..323608
/gene="Tos8"
/locus_tag="P301_G11096"
mRNA 322778..323608
/gene="Tos8"
/locus_tag="P301_G11096"
/product="Tos8p"
CDS 322778..323608
/gene="Tos8"
/locus_tag="P301_G11096"
/note="Similar to YGL096W 'Homeodomain-containing protein
and putative transcription factor found associated with
chromatin; target of SBF transcription factor; induced
during meiosis and under cell-damaging conditions; similar
to Cup9p transcription factor'"
/codon_start=1
/product="Tos8p"
/protein_id="EWG90920.1"
/translation="MGTSIVNLNQKIELPPIQVLFESLNRENETKPHFEERRLYQPNP
SFVPRTNIAVGSPVNPVPVSSPVFFIGPSPQRSIQNHNAIMTQNIRQYPVIYNNNREV
ISTGERNYIITVGGPPVTSSQPEYEHISTPNFYQEQRLAQPHPVNESMMIGGYTNPQP
ISISRGKMLSGNISTNSVRGSNNGYSAKEKKHKAHGKRSNLPKATVSILNKWLHEHVN
NPYPTVQEKRELLAKTGLTKLQISNWFINARRKKIFSGQNDANNFRRKFSSSTNLAKF
"
assembly_gap 323687..323750
/estimated_length=64
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 325444..325545
/estimated_length=102
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 326029..326100
/locus_tag="P301_G11101"
tRNA 326029..326100
/locus_tag="P301_G11101"
/product="tRNA-Glu"
/note="TE(UUC)G1"
gene complement(326052..327784)
/gene="Vps45"
/locus_tag="P301_G11106"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(328295..331642)
/gene="Pan2"
/locus_tag="P301_G11111"
mRNA complement(328295..331642)
/gene="Pan2"
/locus_tag="P301_G11111"
/product="Pan2p"
CDS complement(328295..331642)
/gene="Pan2"
/locus_tag="P301_G11111"
/note="Similar to YGL094C Essential subunit of the
Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to
control poly(A) tail length and regulate the stoichiometry
and activity of postreplication repair complexes"
/codon_start=1
/product="Pan2p"
/protein_id="EWG90921.1"
/translation="MNNWQHFFNNPVDLSEHLKKPYFRFDNRDKEITAISFDEKANLI
WSGDSYGCISSYDPTFQLYTRYRGHIGGNSVKDILSHRDGILSISEDSLHFANRRGVT
KLNLTSIDIAAFSELNTMCYSPHSLKNNIYCGGDNTNWGIASIDLNRGCLDSLLNYSS
KVKLMCSNNKVLSIGRQTGTVDLLDPTSNRTIKSFNAHSASISAMDLRDNTLVTVGKS
KRFYNLYADPFVNVYDLRTMRQLPPVSFSKGTTMGSGGADFVQLHPLLPTVMIVASSS
GSFDFIDLSNPTLRTQYVHPCQSIKKLCLSPNGDVLGILEADNHLDTWRRSSNNMGMF
TNTPEMLAYPDYFNDITSDGPISVDDETYPLSSVGMPYYLDKLLSAWPPVVFKSEGTI
PQLTGKSPLPSSGKLKSNLAVISSQNEKLSTQEFPLLRYDRTKYGMRNAIPDYVCLRD
IRKQITSGLETSDIQTYTSINKYEVPPAYSRLPLTSGRFGTDNFDFTPFNNTEYSGLD
PDVDNHYTNAIIQLYRFIPEMFNFVVGCLKDENFETTLLTDLGYLFDMMERSHGKICS
SSNFQASLKSLTDKRQLENGEPQEHLEEYLESLCIRESIEDFNSSESIKRNMPQKFNR
FLLSQLIKEEAQTVNHNITLNQCFGLETEIRTECSCDHYDTTVKLLPSLSISGINKTV
IKQLNKKSNGQNILPYIEYAMKNVTQKNSICPTCGKTETITQECTVKNLPSVLSLELS
LLDTEFSNIRSSKNWLTSEFYGSIIKNKAVLRSTASELKGTSHIFKYELNGYVAKITD
NNNETRLVTYVKKYNPKENCFKWLMFNDYLVVEITEEEALKMTYPWKTPEIIIYCDAE
ELRKPFFSVDTYSINYDILFRDYFANGIRDTARREYKLLTHDEAPKSGTLVAIDAEFV
SLQSELCEIDHQGIRSIIRPKRTALARISIIRGEEGELYGVPFVDDYVVNTNHIEDYL
TRYSGILPGDLDPEKSTKRLVRRNVVYRKVWLLMQLGCVFVGHGLNNDFKHININVPR
NQIRDTAIYFLQGKRYLSLRYLAYVLLGMNIQEGNHDSIEDAHTALILYKKYLHLKEK
AIFEKVLNSVYEEGRAHNFKVPETSKG"
gene 332063..334816
/gene="Spc105"
/locus_tag="P301_G11116"
mRNA 332063..334816
/gene="Spc105"
/locus_tag="P301_G11116"
/product="Spc105p"
CDS 332063..334816
/gene="Spc105"
/locus_tag="P301_G11116"
/note="Similar to YGL093W 'Subunit of a
kinetochore-microtubule binding complex with Kre28p that
bridges centromeric heterochromatin and kinetochore MAPs
and motors; required for sister chromatid bi-orientation
and kinetochore binding of SAC components'"
/codon_start=1
/product="Spc105p"
/protein_id="EWG90922.1"
/translation="MNVDERSRIGGREKDAGPGKGILKQNQSSQMTSSFLENPGVRIP
TRIITKKEVLDGSNTTSRINTSNLQSMVKRRVSFAPDVTLHSFTFVPEQNNEIKEPRR
RKTSTNSPTKISSQEEPLVTSTQIDDARTEEKTAAEEDPDTSGMELTEPIVATPDSNK
ASQHDPTSMEMTEVFPRSIRQKNPDVEGESIESSQQIDDVEAVREETMELTAIHNVHD
YDSISKDTVEGEPIDLTEYESKPYVPNSVSRSTGKSSDYSVERSNDKSDLSKSENKTN
SSQPMEITDIFHADPQNPMSLHSDNNINNDGNEMELTQIQTNFDRDNHHIDESPSEKH
AFSSNKRRKLDTVSDYAASVTTPVKEAKDTSGEDNDGDLEMMEKMSPITFSDVDNKIG
TRSNDVFTIEPGTEDTGMQTATDDEEDGENVDDNGNKIVEKTRLPEIDKEGQSGIALP
TQDYTLREFINEVGVGFLDTKLIDDLDKKVNFPLNSFNFVENQRIDNVFSAFYIDIPI
LEVEAFRCKELWRSINESKDKFKDFEAQIDKSHPPLLLQEYFSSDEKMKQLMRDQLQL
VKGYSKLEAAMEWYEWRKKQLNGLELILAENLNTLKREYEKLNEEVEKVNSIRGKIRK
LNEAIKEEIRSLKNLPSDSYKPTLMNRIKIEAFKQELMEHSISLSSSNDFTQEMRSLK
LAIAKKSNDILTLRSEVASIDKKIEKRKLFTRFDLPKLRDTLKILESLTGVRFLKFSK
ATLSIAFLQLDDLRVDINLANFKNNPLSSMKVMNDSNNDDMSYHLFTMLLKNVEAEHQ
DSMLSNLFFAMKKWRPLLKYIKLLKLLFPVKITQTEEEEALLQFKDYDRRNKTAFFYV
ISLVSFAQGVFSENGQIPMKVHISTQQDYSPSREVLSDRITHKISGVLPSFTKSRIHL
EFT"
gene 335083..339069
/gene="Nup145"
/locus_tag="P301_G11121"
mRNA 335083..339069
/gene="Nup145"
/locus_tag="P301_G11121"
/product="Nup145p"
CDS 335083..339069
/gene="Nup145"
/locus_tag="P301_G11121"
/note="Similar to YGL092W Essential nucleoporin"
/codon_start=1
/product="Nup145p"
/protein_id="EWG90923.1"
/translation="MFNKSVNSGFTFGNQNTSTPTSTPAQPSSSLQFPQKSTGLFGNV
NVNANTSTPSPSGGLFNANSNANSISQQPANNSLFGNKPAQPSGGLFGATNNTTSKSA
GSLFGNNNATANSTGSTGLFGNNHNSSGGLFSGSNNIASSTQNGGLFGNSNNNNITST
TQNGGLFGKPTTTPAGAGGLFGNSSSTNSTTGLFGSNNTQSSTGIFGQKPGASTTGGL
FGNNGASFPRSGETTGTMSTNPYGINISNVPMAVADMPRSITSSLSDVNGKSDAEPKP
IENRRTYSFSSSVSGNAPLPLASQSSLVSRLSTRLKATQKSTSPNEIFSPSYSKPWLN
GAGSAPLVDDFFSSKMTSLAPNENSNFPQNVFNFLSSQRADLTELRKLKIDSNRSAAK
KLKLLSGTPAITKKHMQDEQDSSKNEPIANADSVTNIDRKENRDNNLDNTYLNGKEQS
NNLNKQDGENTLQHEKSSSFGYWCSPSPEQLERLSLKQLAAVSNFVIGRRGYGCITFQ
HDVDLTAFTKSFREELFGKIVIFRSSKTVEVYPDEATKPMIGHGLNVPAIITLENVYP
VDKKTKKPMKDTTKFAEFQVFDRKLRSMREMNYISYNPFGGTWTFKVNHFSIWGLVNE
EDAEIDEDDLSKQEDGGEQPLRKVRTLAQSKPSDKEVILKTDGTFGTLSGKDDSIVEE
KAYEPDLSDADFEGIEASPKLDVSKDWVEQLILAGSSLRSVFATSKEFDGPCQNEIDL
LFSECNDEIDNAKLIMKERRFTASYTFAKFSTGSMLLTKDIVGKSGVSIKRLPTELQR
KFLFDDIYLDKEIEKVTIEARKSNPYPQISESSLLFKDALDYMEKTSSDYNLWKLSSI
LFDPVSYPYKTDNDQVKMALLKKERHCRLTSWIVSQIGPEIEEKIRNSSNEIEQIFLY
LLLNDVVRASKLAIESKNGHLSVLISYLGSNDPRIRDLAELQLQKWSTGGCSIDKNIS
KIYKLLSGSPFEGLFSLKELESEFSWLCLLNLTLCYGQIDEYSLESLVQSHLDKFSLP
YDDPIGVIFQLYAANENTEKLYKEVRQRTNALDVQFCWYLIQTLRFNGTRVFSKETSD
EATFAFAAQLEFAQLHGHSLFVSCFLNDDKAAEDTIKRLVMREITLLRASTNDHTLNR
LKIPSQLIFNAQALKDRYEGNYLSEVQNLLLGSSYDLAEMAIVTSLGPRLLLSNNPVQ
NNELKTLREILNEFPDSERDKWSVSINVFEVYLKLVLDNVETQETIDSLISGMKIFYD
QYKHCREVAACCNVMSQEIVSKILEKNNPSIGDSKAKLLELPLGQPEKAYLRGEFAQD
LMKCTYKI"
gene complement(339264..340247)
/gene="Nbp35"
/locus_tag="P301_G11126"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 340524..341789
/gene="Lif1"
/locus_tag="P301_G11141"
mRNA 340524..341789
/gene="Lif1"
/locus_tag="P301_G11141"
/product="Lif1p"
CDS 340524..341789
/gene="Lif1"
/locus_tag="P301_G11141"
/note="Similar to YGL090W 'Component of the DNA ligase IV
complex that mediates nonhomologous end joining in DNA
double-strand break repair; physically interacts with
Dnl4p and Nej1p; homologous to mammalian XRCC4 protein'"
/codon_start=1
/product="Lif1p"
/protein_id="EWG90924.1"
/translation="MSQLTEFISCIPVVNEEQNEEDERGLCKIQIEDGAMLETLDENS
LSGLRIEKMLVSEGTGIFSKSSFGINDLRIFTGENIDEESKKYVWYELLKMLTGHKVY
IASLDEKVVFTKWTCRMQDDEVWKVVMELESSAIIRKIAELTLHPVKKGEIDLFEMAD
KLYKDICCVNDSYRNIKESDSSNRNRVEQLARERELLDKLLETRDERTRAMMVTLLNE
KKKKIRELHEILRQNNIKLSDDDVLDSALINTEVQKPISELNSPGKRMKRRKTVVEPQ
NLQKKLKDTSRRRANRKISNQSVIKMEDDDFDDFQFFGLSKRPIITAKDKLSEKYDDI
TSFGDDTQSISFESDSSSDVQKHLVSLEDNGIQISAGRSDEDYGDISGSESETDASAG
EKKSSNHSEQSGNDREPCLQTESETDIET"
gene complement(341996..342358)
/gene="Mf(alpha)2"
/locus_tag="P301_G11146"
mRNA complement(341996..342358)
/gene="Mf(alpha)2"
/locus_tag="P301_G11146"
/product="Mf(alpha)2p"
CDS complement(341996..342358)
/gene="Mf(alpha)2"
/locus_tag="P301_G11146"
/note="Similar to YGL089C 'Mating pheromone alpha-factor,
made by alpha cells; interacts with mating type a cells to
induce cell cycle arrest and other responses leading to
mating; also encoded by MF(ALPHA)1, which is more highly
expressed than MF(ALPHA)2'"
/codon_start=1
/product="Mf(alpha)2p"
/protein_id="EWG90925.1"
/translation="MKFISTFLTFILAAVSVTASSDEDIAQVPAEAIIGYLDFGGDHD
IAFLPFSNATASGLLFINTTIAEAAEKEQNTTLAKREAVADAWHWLNLRPGQPMYKRE
ANADAWHWLQLKPGQPMY"
gene 343191..343435
/locus_tag="P301_G11156"
ncRNA 343191..343435
/ncRNA_class="snoRNA"
/locus_tag="P301_G11156"
/product="SNR10"
gene complement(343611..344109)
/gene="Mms2"
/locus_tag="P301_G11161"
mRNA complement(join(343611..344013,344099..344109))
/gene="Mms2"
/locus_tag="P301_G11161"
/product="Mms2p"
CDS complement(join(343611..344013,344099..344109))
/gene="Mms2"
/locus_tag="P301_G11161"
/note="Similar to YGL087C 'Ubiquitin-conjugating enzyme
variant involved in error-free postreplication repair;
forms a heteromeric complex with Ubc13p, an active
ubiquitin-conjugating enzyme; cooperates with
chromatin-associated RING finger proteins, Rad18p and
Rad5p'"
/codon_start=1
/product="Mms2p"
/protein_id="EWG90926.1"
/translation="MSKVPRNFRLLEELEKGEKGFGPESCSYGLADSDDITMTKWNGT
ILGPPHSNHENRIYSLSIDCGPNYPDSPPKVTFISKINLPCVNPTTGEVQTDFHTLRD
WKRAYTMETLLLDLRKEMATPANKKLRQPKEGETF"
gene 344324..346678
/gene="Mad1"
/locus_tag="P301_G11166"
mRNA 344324..346678
/gene="Mad1"
/locus_tag="P301_G11166"
/product="Mad1p"
CDS 344324..346678
/gene="Mad1"
/locus_tag="P301_G11166"
/note="Similar to YGL086W 'Coiled-coil protein involved in
the spindle-assembly checkpoint; phosphorylated by Mps1p
upon checkpoint activation which leads to inhibition of
the activity of the anaphase promoting complex; forms a
complex with Mad2p'"
/codon_start=1
/product="Mad1p"
/protein_id="EWG90927.1"
/translation="MDVRAALQCFFSALSGRFTGKKLGLEIYSIQYKMSNSGGSSPFL
ESPGGSPDVGSTNGQSNRQIQALQFKLNTLQNEYEIEKLQLQKQTNILEKKYKATIDE
LEKALNDTKYLYESNDKLEQELKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLR
QQYDSKLSKVTNQCDHFKLEAESSHSLLMKYEKEIKRQSVDIKDLQHQVMEKDDELSS
VKASKMINSHPNYSTEEFNELTEMNKMIQDQVQYTKELELANMQQANELKKLKQSQDT
STFWKLENEKLQNKLSQLHVLESQYENLQLENIDLKSKLTKWEIYNDSDDDDDNNVNN
NDNNNNNKNDNNNDNNNDTSNNNNINNNDTSNNNNINNNNRTKNNIRNNPEEIIRDWK
LTKKECLILTDMNDKLRLDNNNLKLLNDEMALERNQILDLNKNYENNIVNLKRLNHEL
EQQKSLSFEECRLLREQLDGLYSAQNNALLEVENSETHASNKNVNEDMNNLIDTYKNK
TEDLTNELKKLNDQLLSNSNDVETQRKKRKLTSDQIGLNYSQRLNELQLENVSVSREL
SKAQTTIQLLQEKLEKLTKLKEKKIRILQLRDGPFIKDQFIKKNKLRLLEKENADLLN
ELKKNNPAVETVPISVYDSLNFELKQFEQEVFKSNKRFSRLKQVFNNKSLEFIDVVNS
LLGFKLEFQQDSRVKIFSCFKPEKYLIADLNENTLKSNLDADIEGWDDLMNLWVEDRG
QLPCFFGNNNIASVGTATSQISVYTFLIDNSNRFYNMINSYPLT"
gene 346855..347678
/locus_tag="P301_G11171"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(347854..349536)
/gene="Gup1"
/locus_tag="P301_G11176"
mRNA complement(347854..349536)
/gene="Gup1"
/locus_tag="P301_G11176"
/product="Gup1p"
CDS complement(347854..349536)
/gene="Gup1"
/locus_tag="P301_G11176"
/note="Similar to YGL084C 'Plasma membrane protein
involved in remodeling GPI anchors; member of the MBOAT
family of putative membrane-bound O-acyltransferases;
proposed to be involved in glycerol transport'"
/codon_start=1
/product="Gup1p"
/protein_id="EWG90928.1"
/translation="MSLISILSPLITSEGLDSRIKPSPKKDASTTTKPSLWKTTEFKF
YYIAFLVVVPLMFYAGLQASSPENPNYARYERLLSQGWLFGRKVDNSDSQYRFFRDNF
ALLSVLMLVHTSIKRIVLYSTNITKLRFDLIFGLIFLVAAHGVNSIRILAHMLILYAI
AHVLKNFRRIATISIWIYGISTLFINDNFRAYPFGNICSFLSPLDHWYRGIIPRWDVF
FNFTLLRVLSYNLDFLERWENLQKKKSPSYESKEAKSAILLNERGRLTAAHPIQDYSL
MNYIAYVTYTPLFIAGPIITFNDYVYQSKHTLPSINFKFIFYYAVRFVIALLSMEFIL
HFLHVVAISKTKAWENDTPFQISMIGLFNLNIIWLKLLIPWRLFRLWALLDGIDTPEN
MIRCVDNNYSSLAFWRAWHRSYNKWVVRYIYIPLGGSKNRVLTSLAVFSFVAIWHDIE
LKLLLWGWLIVLFLLPEIFATQIFSHYTDAVWYRHVCAVGAVFNIWVMMIANLFGFCL
GSDGTKKLLSDMFCTVSGFKFVILASVSLFIAVQIMFEIREEEKRHGIYLKC"
gene 350295..352709
/gene="Scy1"
/locus_tag="P301_G11181"
mRNA 350295..352709
/gene="Scy1"
/locus_tag="P301_G11181"
/product="Scy1p"
CDS 350295..352709
/gene="Scy1"
/locus_tag="P301_G11181"
/note="Similar to YGL083W Putative kinase, suppressor of
GTPase mutant, similar to bovine rhodopsin kinase"
/codon_start=1
/product="Scy1p"
/protein_id="EWG90929.1"
/translation="MMFWSSKTGITSKYSFSSSPTFTAEPWSIYTGRPKSSSSSSPSK
VSIFMFDKKQFENYLLHYGIIKSKSGSRDKVLIQEAYEILRNQANNLAKLKHPNILTL
IEPLEEHSKNFMFVTEFVTSSLETVFRETDDEEQNFLQGHVKDNIVVQRGILQLVNAL
DFVHNRASFVHLNIQPRAIFINENSDWKISGLGYLVKIPPGTNTSEYFLPQYDPRVPP
FMHLQLNYTAPEIVFENTLTFKNDYFSLGLLIYFLYTGKDLFRSENSTSEYKLEYNKF
ESKISTMSWDNIFSKVPQKLRHCIPKLINRDIYSRYDNITLILDSEFFQDPLVKTLNF
LDDLPTKNNEEKYVFLEGLVNLLPEFPPVLLQKKFLPILLELLSQFCAEKVVSDKCVG
KSLDLIIKIGSTLSQLSFQEKVYPVLLSDANFPVLLKKATICLIDNLDTLKQKVKRSD
FLENILKPLFNYVLHDSESDITVVCQEKLLSQIPLALEVLDFPTVKQFLLPLLSNLFT
KTTSLTVKNTCVTCFQIMIEHKSIDSYTCSETVLPLFKSMKTRDPRILSKLLKLFETV
PLIITDEIVLVDQVLPLMWNYSMAPTLTKSQYSGYTKAINKMSSDIQRHHIAKLDDKV
NDIGEDAFHKVIEPTIMKKEDPETVAAKNIEVAAMQPVKKKTGSSYGETSPQSKSILN
SKPLNPKNALATRGFPTRILNSPPQTPSSRTGSKVMTKGGSNDASSTKVEEEFNEFQS
FSSTGSIRQTSAPSDVWMNSTPSPTPTSASSTNLPPGFSISLQPNKRKDGSSDIPRSN
VYGSLI"
gene 353064..354218
/locus_tag="P301_G11191"
mRNA 353064..354218
/locus_tag="P301_G11191"
/product="hypothetical protein"
CDS 353064..354218
/locus_tag="P301_G11191"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90930.1"
/translation="MDVTFLTKNVQINGTQFKILLQNGQGECALIALANVLLISPAHA
RYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINP
EFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEI
QKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFC
TLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATG
QNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNG
YANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKKLCNYVKP"
assembly_gap 354357..354431
/estimated_length=75
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 354615..355574
/locus_tag="P301_G11196"
mRNA 354615..355574
/locus_tag="P301_G11196"
/product="hypothetical protein"
CDS 354615..355574
/locus_tag="P301_G11196"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90931.1"
/translation="MGDIRTFVFAIEDTETTQGLCKTIGRSSSFDQNSLCKPYNLYFD
EPELSRQHAVLCIKTPIPKIEGVPSIEQLRICIRDLNNKTGTVNLVSDGPNDEIDLKN
GDAFGLIAIDNHPFRDNHHLAAKLIFRIELEYFDEAREIVKCTITNVTFGKNNTVSSF
PIHSATSTEDSDSSWYGLSEASTQTEVADECHETNTILTRGGRFSILSLRKRGSKQDQ
KICSNFDRKIHETSSFEEEIEVCTDTDTTEEEEEEEEEKKEGSDEEGKESKXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXSNLDF"
assembly_gap 355422..355554
/estimated_length=133
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 355890..356282
/gene="Fmp37"
/locus_tag="P301_G11201"
mRNA 355890..356282
/gene="Fmp37"
/locus_tag="P301_G11201"
/product="Fmp37p"
CDS 355890..356282
/gene="Fmp37"
/locus_tag="P301_G11201"
/note="Similar to YGL080W 'Putative protein of unknown
function; highly conserved across species and orthologous
to human gene BRP44L; the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Fmp37p"
/protein_id="EWG90932.1"
/translation="MSQPVQRAAARSFLQKYINKETLKYIFTTHFWGPVSNFGIPIAA
IYDLKKDPTLISGPMTFALVTYSGVFMKYALAVSPKNYLLFGCHLINETAQLAQGYRF
LKYTYFTTDEENKALDKEWKEKEKTGKQ"
gene 356698..357354
/locus_tag="P301_G11211"
mRNA 356698..357354
/locus_tag="P301_G11211"
/product="hypothetical protein"
CDS 356698..357354
/locus_tag="P301_G11211"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90933.1"
/translation="MVTGISEENDDEETFSAVHSSTPSINSQSYAIPITEEMSSSFHD
SISTTSNSSGSFDSDGSNVSDVVEQNEMDNESNVDEDLFLDNDIPQSSNLLPTDAQDP
GPIFDVSRYIFDSLKQSIDSADFSEALSLQTKTSAVINSKSLELKQYIDEMKSRLTQL
QEKFENGEATSKKIKRDLETSRKNIDYLNAALRVDFPIEFNQAREKILERRLNEDHDC
"
gene complement(357468..359110)
/gene="Dbp3"
/locus_tag="P301_G11216"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(359475..361166)
/gene="Hnm1"
/locus_tag="P301_G11226"
mRNA complement(359475..361166)
/gene="Hnm1"
/locus_tag="P301_G11226"
/product="Hnm1p"
CDS complement(359475..361166)
/gene="Hnm1"
/locus_tag="P301_G11226"
/note="Similar to YGL077C 'Choline/ethanolamine
transporter; involved in the uptake of nitrogen mustard
and the uptake of glycine betaine during hypersaline
stress; co-regulated with phospholipid biosynthetic genes
and negatively regulated by choline and myo-inositol'"
/codon_start=1
/product="Hnm1p"
/protein_id="EWG90934.1"
/translation="MSIRNDNASGGYMQPDQSSNASMHKRDLRVEEEIKPLDDMDSKG
AVAADGEVHLRKSFSLWSILGVGFGLTNSWFGISTSMVAGISSGGPMMIVYGIIIVAL
ISICIGTSLGELSSAYPHAGGQFWWSLKLAPPKYKRFAAYMCGSFAYAGSVFTSASTT
LSVATEVVGMYALTHPEFIPKRWHIFVCFELLHLFLMFFNCYGKSLPIISSSSLYISL
LSFFTITITVLACSHGKFNDAKFVFATFNNETGWKNGGIAFIVGLINPAWSFSCLDCA
THMAFEVEKPERVIPIAIMGTVAIGFVTSFCYVIAMFFSIQDLDAVLSSTTGAPILDI
YNQALGNKSGAIFLGCLILFTSFGCVIACHTWQARLCWSFARDNGLPLSRLWSQVNPH
TGVPLNAHLMSCAWITLIGLLYLASSTAFQSLITGCIAFLLLSYIIPVICLLAKKRNI
AHGPFWLGKFGFFSNIVLLGWTVFSVVFFSFPPVLPVTKDNMNYVCVVIVGYTAYSIL
YWKYKGKKEFHALEESENEQAEYSNNFDTIEDSREFSVAASDVELENEHVPWGKK"
gene complement(361584..363245)
/gene="Rpl7a"
/locus_tag="P301_G11231"
mRNA complement(join(361584..362213,362682..362775,
363235..363245))
/gene="Rpl7a"
/locus_tag="P301_G11231"
/product="Rpl7ap"
CDS complement(join(361584..362213,362682..362775,
363235..363245))
/gene="Rpl7a"
/locus_tag="P301_G11231"
/note="Similar to YGL076C 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl7Bp and
has similarity to E. coli L30 and rat L7 ribosomal
proteins; contains a conserved C-terminal Nucleic acid
Binding Domain (NDB2)'"
/codon_start=1
/product="Rpl7ap"
/protein_id="EWG90935.1"
/translation="MAAEKILTPESQLKKSKAQQKTAEQVAAERAARKAANKEKRAII
LERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKV
LQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQR
VPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPR
KFKHFIQGGSFGNREEFINKLVKSMN"
gene complement(362412..362500)
/locus_tag="P301_G11236"
ncRNA complement(362412..362500)
/ncRNA_class="snoRNA"
/locus_tag="P301_G11236"
/product="SNR39"
gene complement(363617..363712)
/locus_tag="P301_G11241"
ncRNA complement(363617..363712)
/ncRNA_class="snoRNA"
/locus_tag="P301_G11241"
/product="SNR39B"
gene complement(364164..365327)
/gene="Mps2"
/locus_tag="P301_G11246"
mRNA complement(364164..365327)
/gene="Mps2"
/locus_tag="P301_G11246"
/product="Mps2p"
CDS complement(364164..365327)
/gene="Mps2"
/locus_tag="P301_G11246"
/note="Similar to YGL075C 'Essential membrane protein
localized at the nuclear envelope and spindle pole body
(SPB), required for insertion of the newly duplicated SPB
into the nuclear envelope; potentially phosphorylated by
Cdc28p'"
/codon_start=1
/product="Mps2p"
/protein_id="EWG90936.1"
/translation="MSNGAFDAIFEYAWGQIDKPISGDFIYGKDLPKLIEIIENIFQK
AQKSGSYELRLPLFSEINKDLFRTFSNTKTFFKIHKEEFDDIFFNLVNHPLREILENA
FIGVDSIPSDFIVSMNLNSPSKFLVENKSKNTEGAGISTPRKKLTESPIKLLSRNNIG
KALEVQVEELKRELTAKQSLLQENERQVSELKIRLETYQEKYASIQQRFSDLQKARQV
EDNQNSSRTSDPGSPLVTGIDQKAILEEFRRRLQRQTDTISFLKDQIRRERGLNCSND
KVSHSKRKHATTDGDGTFKNFISAVPSNIWVKATIRIIVCFALLAGVLPYIRKYVYAH
DTPSQNSRLQLSWWENSGILSKIVWFFEDQTDLETEYRSNANVDDAYSRVFGI"
gene 365993..368494
/gene="Hsf1"
/locus_tag="P301_G11256"
mRNA 365993..368494
/gene="Hsf1"
/locus_tag="P301_G11256"
/product="Hsf1p"
CDS 365993..368494
/gene="Hsf1"
/locus_tag="P301_G11256"
/note="Similar to YGL073W 'Trimeric heat shock
transcription factor, activates multiple genes in response
to stresses that include hyperthermia; recognizes variable
heat shock elements (HSEs) consisting of inverted NGAAN
repeats; posttranslationally regulated'"
/codon_start=1
/product="Hsf1p"
/protein_id="EWG90937.1"
/translation="MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTT
DRTDASTTSSTAIEDIINPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIY
QTNLYGHNSRENTNPNSTLLSSKLLAHPPVPYGQNPNLLQHAVYRAQPSSGTTNAQPR
QTTRRYQSHKSRPAFVNKLWSMLNDDSNTKLIQWAEDGKSFIVTNREEFVHQILPKYF
KHSNFASFVRQLNMYGWHKVQDVKSGSIQSSSDDKWQFENENFIRGREDLLEKIIRQK
GSSNNHSSPSGNGNPANGSNIPLDNAAGINNSNNNISSSNSFFNNGHLLQGKTLRLMN
EANLGDKNDVTAILGELEQIKYNQIAISKDLLRINKDNELLWKENMMARERHRTQQQA
LEKMFRFLTSIVPHLDPKMIMDGLGDPKVNNEKLNSANNIGLNRDNTGTIDELKSNDS
FINDDRNSFTNATTNARNNMSPNNDDNSIDTASTNTTNRKKNIDENIKNNNDIINDII
FNTNLANNLSNYNSNNNAGSPIRPYKQRYLLKNRANSSTSSENPSLTPFDIESNNDRK
ISEIPFDDGEEEETDFRPFTSRDPNNQTSENTFDPNRFTMLSDDDLKKDSHTNDNKHN
ESDLFLDNVHRNIDEQDARLQNLENMVHILSPGYPNKSFNNKTSSTNTNSNMESAVNV
NSPGFNLQDYLTGESNSPNSVHSVPSNGSGSTPLPMPNDNDTEHASTSVNQGENGSGL
TPFLTVDDHTLNDNNTSEGSTRVSPDIKFSATENTKVSDNLPSFNDHSYSTQADTAPE
NAKKRFVEEIPEPAIVEIQDPTEYNDHRLPKRAKK"
gene 369251..371323
/gene="Aft1"
/locus_tag="P301_G11266"
mRNA 369251..371323
/gene="Aft1"
/locus_tag="P301_G11266"
/product="Aft1p"
CDS 369251..371323
/gene="Aft1"
/locus_tag="P301_G11266"
/note="Similar to YGL071W 'Transcription factor involved
in iron utilization and homeostasis; binds the consensus
site PyPuCACCCPu and activates the expression of target
genes in response to changes in iron availability'"
/codon_start=1
/product="Aft1p"
/protein_id="EWG90938.1"
/translation="MEGFNPAHIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGR
ASASGNPAAVPSPIMTLNLKSTHSLNSDQHVHTSTSPTETIGHIHHVEKLNQNNLIHL
DPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDTFKVVFKCKAAKRGRNARRKRKDKP
KGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFR
VRATYSLKRKRWSIVVMNNNHSHQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEY
RTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSSTVSSISLDSSNASKR
PCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHS
STAFSGYIDDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQV
FDQLDDCSSILFSPLTTNTNNEFEGESDDFVHSPYLNSEADFSQILSSAPPVHHDPNE
THQENQDIIDRFANSSQEHNEYILQYLTHFDAANHNNIGVPNNNSHSLNTQHNVSDLG
NPLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYG
PQQQPPQQLQYHQNQPHDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGE
LKYVKPED"
gene complement(371698..372066)
/gene="Rpb9"
/locus_tag="P301_G11271"
mRNA complement(371698..372066)
/gene="Rpb9"
/locus_tag="P301_G11271"
/product="Rpb9p"
CDS complement(371698..372066)
/gene="Rpb9"
/locus_tag="P301_G11271"
/note="Similar to YGL070C 'RNA polymerase II subunit
B12.6; contacts DNA; mutations affect transcription start
site selection and fidelity of transcription'"
/codon_start=1
/product="Rpb9p"
/protein_id="EWG90939.1"
/translation="MTTFRFCRDCNNMLYPREDKENNRLLFECRTCSYVEEAGSPLVY
RHELITNIGETAGVVQDIGSDPTLPRSDRECPKCHSRENVFFQSQQRRKDTSMVLFFV
CLSCSHIFTSDQKNKRTQFS"
gene 372326..372910
/gene="Mnp1"
/locus_tag="P301_G11281"
mRNA 372326..372910
/gene="Mnp1"
/locus_tag="P301_G11281"
/product="Mnp1p"
CDS 372326..372910
/gene="Mnp1"
/locus_tag="P301_G11281"
/note="Similar to YGL068W 'Protein associated with the
mitochondrial nucleoid; putative mitochondrial ribosomal
protein with similarity to E. coli L7/L12 ribosomal
protein; required for normal respiratory growth'"
/codon_start=1
/product="Mnp1p"
/protein_id="EWG90940.1"
/translation="MSLRILAKRSSSIWMKTRVTPALISPITITTRFNSTTTTAPSHK
DDVRPVDPKISKIVQDISQLTLLETSSLINELKTVLNIPEISMPMGGFMAGAAGAGAG
NVPSSTGEAGSGAEEEAKPEAKTVFTVKLDSFDTKTKAKVIKEVKGLLGLSLVEAKKF
VEAAPKVLKENVAKDDAEKIKKTLEDLGAKVSLE"
gene 373339..374492
/gene="Npy1"
/locus_tag="P301_G11286"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 374859..376821
/gene="Sgf73"
/locus_tag="P301_G11291"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 376090..376123
/estimated_length=34
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(376999..378510)
/gene="Alg2"
/locus_tag="P301_G11296"
mRNA complement(376999..378510)
/gene="Alg2"
/locus_tag="P301_G11296"
/product="Alg2p"
CDS complement(376999..378510)
/gene="Alg2"
/locus_tag="P301_G11296"
/note="Similar to YGL065C 'Mannosyltransferase that
catalyzes two consecutive steps in the N-linked
glycosylation pathway; alg2 mutants exhibit
temperature-sensitive growth and abnormal accumulation of
the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol'"
/codon_start=1
/product="Alg2p"
/protein_id="EWG90941.1"
/translation="MIEKDKRTIAFIHPDLGIGGAERLVVDAALGLQQQGHSVIIYTS
HCDKSHCFEEVKNGQLKVEVYGDFLPTNFLGRFFIVFATIRQLYLVIQLILQKKVNAY
QLIIIDQLSTCIPLLHIFSSATLMFYCHFPDQLLAQRAGLLKKIYRLPFDLIEQFSVS
AADTVVVNSNFTKNTFHQTFKYLSNDPDVIYPCVDLSTIENEDIDKKFFKTVFNEGDR
FYLSINRFEKKKDVALAIKAFALSEDQINDNVKLVICGGYDERVAENVEYLKELQSLA
DEYELPHTTIYYQEIKRVSDLESFKANNSKIIFLTSISSSLKELLLERTEMLLYTPAY
EHFGIVPLEAMKLGKPVLAVNNGGPLETIKSYVAGENESSATGWLKPAVPIQWATAID
ESRKILQNGSVNFERNGPLRVKKYFSREAMTQSFEENVEKVIWKEKKYYPWEIFGISF
SNFILHMAFIKILPNNPWPFLFMATFMVLYFKNYLWGIYWAFVFALSYPYEEI"
gene complement(378762..380447)
/gene="Mrh4"
/locus_tag="P301_G11301"
mRNA complement(378762..380447)
/gene="Mrh4"
/locus_tag="P301_G11301"
/product="Mrh4p"
CDS complement(378762..380447)
/gene="Mrh4"
/locus_tag="P301_G11301"
/note="Similar to YGL064C Mitochondrial ATP-dependent RNA
helicase of the DEAD-box family, plays an essential role
in mitochondrial function"
/codon_start=1
/product="Mrh4p"
/protein_id="EWG90942.1"
/translation="MSLFFKPVISPQWSFPVLLKIGVRSYAGGPRTKHKGNSPLASVP
TGSSNKNRKQKAKGKKGNKKNDPDQAFNFGEYGGLKKDVEMNMDSTNKLIQKISNFDQ
LLILPPVRDAVKEIISKESLKLQDSRKKTSENIIPSPIQTVAIKRISKNLMDPKLQIH
AIAAETGSGKTMAYLIPLIDYLKRQELETPELWETLRKNVLIRSIILVPTHELVDQVY
ETVSKTKTLLGLNSFKWDKATSYRDLLENIKNRIDILVTTPGKLLNLFSIRMITRPDK
VLSKVGFVVLDEADTLLDRSWLEETHSAIKRIPNINHLIFCSATIPQEFNKTMQRLFP
TVVPIMTPRLHKLPFALDFKVINSALSPFKGSKIKALAQTLYAISNDDTEPGFEKRCI
IFVNEKKNVPEIVNLLNKKFGHNAIGLTGEDTFEERSEKIMPFLSPPRPLSEVVAQST
SPPTSLKKFEIPDSNIVIGKLKNTNSNGTAPSNKSLHVLVTTDLMARGLNFKGVRNVV
LYDVPKTSIDLIHRVGRTARMKQGGRVFMLTDSKTKSWAKALPKIIKKHQRLS"
gene 380895..382007
/gene="Pus2"
/locus_tag="P301_G11306"
mRNA 380895..382007
/gene="Pus2"
/locus_tag="P301_G11306"
/product="Pus2p"
CDS 380895..382007
/gene="Pus2"
/locus_tag="P301_G11306"
/note="Similar to YGL063W 'Mitochondrial
tRNA:pseudouridine synthase; acts at positions 27 and 28,
but not at position 72; efficiently and rapidly targeted
to mitochondria, specifically dedicated to mitochondrial
tRNA modification'"
/codon_start=1
/product="Pus2p"
/protein_id="EWG90943.1"
/translation="MLLGYCGSGYYGMQYNPPHKTIEGEILTKLFDVGAISEENSLAP
KKNSFMAAARTDKGVHAMLNLLSLKITLREDTVAKLNAALPPEIRVWGIQPVNKKFNA
RSACDSRWYQYLIPEFILIGPPRSSLLHRNVGGCYREDGSQEVWDTFLEQTRGRFSGD
ELCRLQDTAQKLSESDPLVQDYVGLLSGTLSGYCLPPSKLDAFEAAMQEYVGTHNFHN
FTTGKLWGDPSAQRHIKKVVVSQASPGWICVRIHGQSFMLHQIRRMVALAVLAARCQL
PPNIVRNYFNAGPRKYIPRAPAQGLLLEGPVFDGYNTKLRNLLYCEIRPDDITLERMC
RFRERQICTAIAHEETQRHVFCHFVRQMNRLATPLI"
gene 382437..385974
/gene="Pyc1"
/locus_tag="P301_G11316"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 385212..385244
/estimated_length=33
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(386208..386951)
/gene="Duo1"
/locus_tag="P301_G11326"
mRNA complement(386208..386951)
/gene="Duo1"
/locus_tag="P301_G11326"
/product="Duo1p"
CDS complement(386208..386951)
/gene="Duo1"
/locus_tag="P301_G11326"
/note="Similar to YGL061C 'Essential subunit of the Dam1
complex (aka DASH complex), couples kinetochores to the
force produced by MT depolymerization thereby aiding in
chromosome segregation; is transferred to the kinetochore
prior to mitosis'"
/codon_start=1
/product="Duo1p"
/protein_id="EWG90944.1"
/translation="MSEQSQLDDSTIDKLIPQIFNEMRSNLNNTTNKFPKSTGGGASD
NISANSNSIRSFNSITTQSLLKESESLDKITAMIKNVTAALKNNLPVYVNQVHEVCKS
TNSILDSWINIHSQAGYIHKLMSDQTYLKLINDRLHNENVNTNDEDGSTLHNVIALKK
KEILDLRQKLENRKGEKDAAPAKPPNQGLNPRYGVQSGRRPVSSAGISNNGRVRKTHV
PASKRPSGIPRVTNRWTKPTASSSRKMFR"
gene 387305..389230
/gene="Ybp2"
/locus_tag="P301_G11331"
mRNA 387305..389230
/gene="Ybp2"
/locus_tag="P301_G11331"
/product="Ybp2p"
CDS 387305..389230
/gene="Ybp2"
/locus_tag="P301_G11331"
/note="Similar to YGL060W 'Central kinetochore associated
protein that mediates mitotic progression; interacts with
several central kinetochore proteins and the centromeric
histone Cse4p; role in resistance to oxidative stress;
similarity to Ybp1p and Slk19p'"
/codon_start=1
/product="Ybp2p"
/protein_id="EWG90945.1"
/translation="MYNEQVNSGKSIKEKERYLDALLKILKDNPVTLKEIGWDLPKGL
LQFFSRKNINVNIHLVFSPLVSSVMECFNELAINGNPKECLLTACELVSTLHIVLTET
GDSDEENEDLNDSNRNDASNITDELSVITPEIGQYMANNTVEFIPNLKIYVLFEFMSL
LLKRVDTLYPSKFLAMVTSAIIKYVTTNVQAMDDPHFILRIVYNFCTNYSPAQPSASL
TDGISTNDLEKIHDDESALQKKLLANLSVFVISNCLKNHPGNIDKIYFKTLMHKKTDE
NEIDASVLQTCHQYYEYVTSLDVHMKELLEKCLVESRSIYNSLLMNPAASTPEFKEEI
NQLVYEVSYAYQIKKLADEKNLELDQYGVVILSAIHYSKNGTHLLPQIDIQSAIYLYL
RCTTASLFSEIYENKFLESSVRYWLWVSTTETSTEKIKCALQELPGHITTAFLQMLLM
KTCNESNNDTKLTEITLLRRLLYLMPESTSFTFIFETLLHCPYITAKIAVLDILRDMM
IRSPEAANRDETVGLIEQQNPGNTANSVPIMPTLPPRPYITINEDRMASIHSIALICF
SAAKQKKRTQGDLLLVLTYMKFFVSLRNKWDLGLLTLINKEISESFQGEGEPELAFIN
ISNNTLGEYIEEMNIRS"
gene 389463..390938
/locus_tag="P301_G11336"
mRNA 389463..390938
/locus_tag="P301_G11336"
/product="hypothetical protein"
CDS 389463..390938
/locus_tag="P301_G11336"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90946.1"
/translation="MSKYQINCIRYRHFLRTSNISQIPDFTKYCIGPVNEELAPYIME
TMKAYPSNSEYINPQHYYHNRTVLVENYLKRSPNPVSLTQLAQYYDDSTKLTRTKIIN
SGKFVKEELVIRIAHKLNQLQQLPFNVVNNFHFVQVYESYYNIFESFRKYPTIRTLED
ASQFADFIKNMLEGFNTLNLPHLIMGALECTILDLYPREKMDQLLSDLLRARISRRLI
VEEHVSITANYTSGKEENTLVLGDIFQECSAKKYLLEASEESQKFIQDMYFKDIPMPE
FIIEGDTQLSFYFLPTHLKYLLGEILRNTYEATMKHYIRKGLEKPEPIIVTVVSNDES
YLFRISDKAGGVLHDDENLWSFGKSKERAQESLNNFHKLPGLQTVSIYDEVHSHTKYN
SKLKSLQSITLKPYMHTSLEPMSYPSIINGHIKYETPLIELLKRSFRYKLGIGLAMCK
VYAEYWNGDLSLHSMPGYGTDVVLKLGNLMKHTKKLQLDKV"
gene 391226..391744
/gene="Rad6"
/locus_tag="P301_G11341"
mRNA 391226..391744
/gene="Rad6"
/locus_tag="P301_G11341"
/product="Rad6p"
CDS 391226..391744
/gene="Rad6"
/locus_tag="P301_G11341"
/note="Similar to YGL058W Ubiquitin-conjugating enzyme
(E2), involved in postreplication repair (as a heterodimer
with Rad18p), DSBR and checkpoint control (as a
heterodimer with Bre1p), ubiquitin-mediated N-end rule
protein degradation (as a heterodimer with Ubr1p"
/codon_start=1
/product="Rad6p"
/protein_id="EWG90947.1"
/translation="MSTPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPA
DTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDV
ASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWEDDMDDMDDDD
DDDDDDDDDEAD"
gene complement(391881..392987)
/locus_tag="P301_G11346"
mRNA complement(391881..392987)
/locus_tag="P301_G11346"
/product="hypothetical protein"
CDS complement(391881..392987)
/locus_tag="P301_G11346"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90948.1"
/translation="MVLSNVKIFRLKSHRAFRIGPMIKAVAGNLLVKRFYQPKLERIP
PASLLLKQKIRLAQNGSTTSTENPISFSQTMSEIFSVLQPSAPDLDEDETSGLKRDHL
LTERLNNGELGVIMNKFFNPSSTHNNQLIDTNILLQNFPKLSGNDLDLLDFAINEKMR
GNWNDLKQDFIQLWYYKSFGFLGPRTQFVLTNSSPSLRSQFLKLPFIEYNWFLLQNNK
NANILPADVQNVVKVFHLDDKRFSWKSIDPFSKAIISFVVFVSIYVWLDESAKQKTKE
LPAQKSTVISEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXT
LFSPKKFMVEQCFFLVYYLYAMFLSLLNFLYGIK"
assembly_gap 391990..392124
/estimated_length=135
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(393191..394774)
/gene="Sds23"
/locus_tag="P301_G11351"
mRNA complement(393191..394774)
/gene="Sds23"
/locus_tag="P301_G11351"
/product="Sds23p"
CDS complement(393191..394774)
/gene="Sds23"
/locus_tag="P301_G11351"
/note="Similar to YGL056C 'One of two S. cerevisiae
homologs (Sds23p and Sds24p) of the S. pombe Sds23
protein, which is implicated in APC/cyclosome regulation;
involved in cell separation during budding'"
/codon_start=1
/product="Sds23p"
/protein_id="EWG90949.1"
/translation="MPQNTRHTSIVEMLSTPPQLPNSTDLNSLSEQTDKNTEANKSDT
ESLHKSISKSSSSSSLSTLDNTEYSNNNGNSLSTLNSQNLLSVHRQEWQHTPLSNLVE
QNKLIFIRGSISVEEAFNTLVKHQLTSLPVENFPGDMNCLTFDYNDLNAYLLLVLNRI
KVSNDKITSDCQNGKSVPVGEIVKLTPKNPFYKLPETENLSTVIGILGSGVHRVAITN
VEMTQIKGILSQRRLIKYLWENARSFPNLKPLLDSSLEELNIGVLNAARDKPTFKQSR
VISIQGDEHLIMALHKMYVERISSIAVVDPQGNLIGNISVTDVKHVTRTSQYPLLHNT
CRHFVSVILNLRGLETGKDSFPIFHVYPTSSLARTFAKLVATKSHRLWIVQPNDNQPT
ASSEKSSSPSPSTPPVTTLPSLASSYHSNTQSSRMANSPVLKSSDTSNNKINVNINLS
GPSPSQPQSPSTAMPPPQSPSNCPASPTPAHFEKEYRTGKLIGVVSLTDILSVLARKQ
THHKEIDPQMARKQRGHIG"
gene 395779..397311
/gene="Ole1"
/locus_tag="P301_G11361"
mRNA 395779..397311
/gene="Ole1"
/locus_tag="P301_G11361"
/product="Ole1p"
CDS 395779..397311
/gene="Ole1"
/locus_tag="P301_G11361"
/note="Similar to YGL055W Delta(9) fatty acid desaturase,
required for monounsaturated fatty acid synthesis and for
normal distribution of mitochondria"
/codon_start=1
/product="Ole1p"
/protein_id="EWG90950.1"
/translation="MPTSGTTIELIDDQFPKDDSASSGIVDEVDLTEANILATGLNKK
APRIVNGFGSLMGSKEMVSVEFDKKGNEKKSNLDRLLEKDNQEKEEAKTKIHISEQPW
TLNNWHQHLNWLNMVLVCGMPMIGWYFALSGKVPLHLNVFLFSVFYYAVGGVSITAGY
HRLWSHRSYSAHWPLRLFYAIFGCASVEGSAKWWGHSHRIHHRYTDTLRDPYDARRGL
WYSHMGWMLLKPNPKYKARADITDMTDDWTIRFQHRHYILLMLLTAFVIPTLICGYFF
NDYMGGLIYAGFIRVFVIQQATFCINSMAHYIGTQPFDDRRTPRDNWITAIVTFGEGY
HNFHHEFPTDYRNAIKWYQYDPTKVIIYLTSLVGLAYDLKKFSQNAIEEALIQQEQKK
INKKKAKINWGPVLTDLPMWDKQTFLAKSKENKGLVIISGIVHDVSGYISEHPGGETL
IKTALGKDATKAFSGGVYRHSNAAQNVLADMRVAVIKESKNSAIRMASKRGEIYETGK
FF"
assembly_gap 397348..397726
/estimated_length=379
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(398045..398461)
/gene="Erv14"
/locus_tag="P301_G11366"
mRNA complement(398045..398461)
/gene="Erv14"
/locus_tag="P301_G11366"
/product="Erv14p"
CDS complement(398045..398461)
/gene="Erv14"
/locus_tag="P301_G11366"
/note="Similar to YGL054C 'Protein localized to
COPII-coated vesicles, involved in vesicle formation and
incorporation of specific secretory cargo; required for
the delivery of bud-site selection protein Axl2p to cell
surface; related to Drosophila cornichon'"
/codon_start=1
/product="Erv14p"
/protein_id="EWG90951.1"
/translation="MGAWLFILAVVVNCINLFGQVHFTILYADLEADYINPIELCSKV
NKLITPEAALHGALSLLFLLNGYWFVFLLNLPVLAYNLNKIYNKVQLLDATEIFRTLG
KHKRESFLKLGFHLLMFFFYLYRMIMALIAESGDDF"
gene complement(398702..398773)
/locus_tag="P301_G11371"
tRNA complement(398702..398773)
/locus_tag="P301_G11371"
/product="tRNA-Glu"
/note="TE(UUC)G2"
assembly_gap 399023..399653
/estimated_length=631
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 399827..399898
/locus_tag="P301_G11376"
tRNA 399827..399898
/locus_tag="P301_G11376"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg3);
TR(UCU)G1"
gene 400132..400953
/gene="Tyw3"
/locus_tag="P301_G11381"
mRNA 400132..400953
/gene="Tyw3"
/locus_tag="P301_G11381"
/product="Tyw3p"
CDS 400132..400953
/gene="Tyw3"
/locus_tag="P301_G11381"
/note="Similar to YGL050W tRNA methyltransferase required
for synthesis of wybutosine, a modified guanosine found at
the 3'-position adjacent to the anticodon of phenylalanine
tRNA which supports reading frame maintenance by
stabilizing codon-anticodon interactions"
/codon_start=1
/product="Tyw3p"
/protein_id="EWG90952.1"
/translation="MAAQNAFEQKKRAILNEIDSTQPDLSPKGTIDELCLPIIDLINA
SADMVTTSSCSGRVSVFLEGTKSYNGEVKIGGKGQGGKWLYVTHDREKVIGWLDELKS
KSEFSFELSGKEIPTEKVTGSIRYILYKYEPFILHVKCRDFQAASKLYNTAMSCGFRE
SGIGSNNLVAIRINIKLDVPLGYLDETSGTLKFFVTPEYVSVLDSLSLSKFDENTRKM
QALYDRIEKELINCAPDVNSKVNIMPIETKEERRERKKREGMERQRQLKSPQNVL"
gene complement(401215..403961)
/gene="Tif4632"
/locus_tag="P301_G11386"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(404425..405642)
/gene="Rpt6"
/locus_tag="P301_G11391"
mRNA complement(404425..405642)
/gene="Rpt6"
/locus_tag="P301_G11391"
/product="Rpt6p"
CDS complement(404425..405642)
/gene="Rpt6"
/locus_tag="P301_G11391"
/note="Similar to YGL048C 'One of six ATPases of the 19S
regulatory particle of the 26S proteasome involved in the
degradation of ubiquitinated substrates; bound by
ubiquitin-protein ligases Ubr1p and Ufd4p; localized
mainly to the nucleus throughout the cell cycle'"
/codon_start=1
/product="Rpt6p"
/protein_id="EWG90953.1"
/translation="MTAAVTSSNIVLETHESGIKPYFEQKIQETELKIRSKTENVRRL
EAQRNALNDKVRFIKDELRLLQEPGSYVGEVIKIVSDKKVLVKVQPEGKYIVDVAKDI
NVKDLKASQRVCLRSDSYMLHKVLENKADPLVSLMMVEKVPDSTYDMVGGLTKQIKEI
KEVIELPVKHPELFESLGIAQPKGVILYGPPGTGKTLLARAVAHHTDCKFIRVSGAEL
VQKYIGEGSRMVRELFVMAREHAPSIIFMDEIDSIGSTRVEGSGGGDSEVQRTMLELL
NQLDGFETSKNIKIIMATNRLDILDPALLRPGRIDRKIEFPPPSVAARAEILRIHSRK
MNLTRGINLRKVAEKMNGCSGADVKGVCTEAGMYALRERRIHVTQEDFELAVGKVMNK
NQETAISVAKLFK"
gene 405908..406516
/gene="Alg13"
/locus_tag="P301_G11396"
mRNA 405908..406516
/gene="Alg13"
/locus_tag="P301_G11396"
/product="Alg13p"
CDS 405908..406516
/gene="Alg13"
/locus_tag="P301_G11396"
/note="Similar to YGL047W 'Catalytic component of
UDP-GlcNAc transferase, required for the second step of
dolichyl-linked oligosaccharide synthesis; anchored to the
ER membrane via interaction with Alg14p; similar to
bacterial and human glycosyltransferases'"
/codon_start=1
/product="Alg13p"
/protein_id="EWG90954.1"
/translation="MGIIEEKTLFVTCGATVPFPKLVSCVLSDEFCQELIQYGFVRLI
IQFGRNYSSEFEHLVQERGGQRESQKIPIDQFGCGDTARQYVLMNGKLKVIGFDFSTK
MQSIIRDYSDLVISHAGTGSILDSLRLNKPLIVCVNDSLMDNHQQQIADKFVELGYVW
SCAPTETGLIAGLRASQTEKLKPFPVSHNPSFERLLVETIYS"
gene complement(406646..406719)
/locus_tag="P301_G11401"
tRNA complement(406646..406719)
/locus_tag="P301_G11401"
/product="tRNA-Val"
/note="TV(AAC)G1"
gene 408465..410093
/gene="Rim8"
/locus_tag="P301_G11411"
mRNA 408465..410093
/gene="Rim8"
/locus_tag="P301_G11411"
/product="Rim8p"
CDS 408465..410093
/gene="Rim8"
/locus_tag="P301_G11411"
/note="Similar to YGL045W 'Protein involved in proteolytic
activation of Rim101p in response to alkaline pH;
interacts with ESCRT-1 subunits Stp22p and Vps28p;
essential for anaerobic growth; member of the
arrestin-related trafficking adaptor family'"
/codon_start=1
/product="Rim8p"
/protein_id="EWG90955.1"
/translation="MSLLRLWNKESRAPSKIKSHGIVGCYGNSMLAHNNVKQFRIDID
EPHRVWKPNENITGEAVIDIKRDITNVAIKLSLVCEVRVKTGNSPTSKNKRIEKTLEK
STFLYGQDYVKTAFSAKEKKPHVDKTTILNGLSKGEHRFPFRIRIPRGRGMLSSIKFE
RGSITYFLSCTLESLNNINGLKKPEARCEREFAVIVPLDVSRLPKPKIKTVVLQSASM
VQNKKNKSTEDESSSYTQLTQKSTTSNSSSSSVNSKTSPLPNKTVTISVDIPQAGFMI
GEIIPIDVKIDHYKPFYAPAGLTTTLVRICRVGGAGKDDPMETFRKDICQSISPIYIN
PETLQFQSRVYLKVPLDAFSTLTTVGKFFSFQYYIEVMVNLSKKNVVYTESNRIIGTP
IGEQNGLGVENNINRIQRKMLRMVNPETLENDSEGYESSIFFKDMVNVEKLKRLRNVT
GMSIETVIGTTRSEQQQSDASIPSQSSITAPQNSPSNLRDWLAPLNAYDSDDVPVPKY
SPNDKVSVPSEDKQELEQKRLQQLESDPPPCDDY"
gene complement(410506..411393)
/gene="Rna15"
/locus_tag="P301_G11416"
mRNA complement(410506..411393)
/gene="Rna15"
/locus_tag="P301_G11416"
/product="Rna15p"
CDS complement(410506..411393)
/gene="Rna15"
/locus_tag="P301_G11416"
/note="Similar to YGL044C 'Cleavage and polyadenylation
factor I (CF I) component involved in cleavage and
polyadenylation of mRNA 3' ends; interacts with the A-rich
polyadenylation signal in complex with Rna14p and Hrp1p'"
/codon_start=1
/product="Rna15p"
/protein_id="EWG90956.1"
/translation="MNRQSGVNAGVQNNPPSRVVYLGSIPYDQTEEQILDLCSNVGPV
INLKMMFDPQTGRSKGYAFIEFRDLESSASAVRNLNGYQLGSRFLKCGYSSNSDISGV
SQQQQQQYNNINGNNNNNGNNNNSNGPDFQNSGNANFLSQKFPELPSGIDVNINMTTP
AMMISSELAKKPKEVQLKFLQKFQEWTRAHPEDAVSLLELCPQLSFVTAELLLTNGIC
KVDDLIPLASRPQEEASATNNNSVNEVVDPAVLNKQKELLKQVLQLNDSQISILPDDE
RMAIWDLKQKALRGEFGAF"
gene 411839..412768
/gene="Dst1"
/locus_tag="P301_G11421"
mRNA 411839..412768
/gene="Dst1"
/locus_tag="P301_G11421"
/product="Dst1p"
CDS 411839..412768
/gene="Dst1"
/locus_tag="P301_G11421"
/note="Similar to YGL043W General transcription elongation
factor TFIIS, enables RNA polymerase II to read through
blocks to elongation by stimulating cleavage of nascent
transcripts stalled at transcription arrest sites"
/codon_start=1
/product="Dst1p"
/protein_id="EWG90957.1"
/translation="MDSKEVLVHVKNLEKNKSNDAAVLEILHVLDKEFVPTEKLLRET
KVGVEVNKFKKSTNVEISKLVKKMISSWKDAINKNKRSRQAQQHHQDHAPGNAEDKTT
VGESVNGVQQPASSQSDAMKQDKYVSTKPRNSKNDGVDTAIYHHKLRDQVLKALYDVL
AKESEHPPQSILHTAKAIESEMNKVNNCDTNEAAYKARYRIIYSNVISKNNPDLKHKI
ANGDITPEFLATCDAKDLAPAPLKQKIEEIAKQNLYNAQGATIERSVTDRFTCGKCKE
KKVSYYQLQTRSADEPLTTFCTCEACGNRWKFS"
gene complement(413060..413242)
/locus_tag="P301_G11431"
mRNA complement(413060..413242)
/locus_tag="P301_G11431"
/product="hypothetical protein"
CDS complement(413060..413242)
/locus_tag="P301_G11431"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90958.1"
/translation="MFDSSIERVTLELCFHITLSIMCGCSIYFLLLVFILTFYSSVLL
HLKLYFFSSDRAIFNA"
gene 413181..413645
/locus_tag="P301_G11441"
mRNA 413181..413645
/locus_tag="P301_G11441"
/product="hypothetical protein"
CDS 413181..413645
/locus_tag="P301_G11441"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90959.1"
/translation="MLRVIWKHSSRVTRSIELSNISTTNHTRSLRRLSWISPRRFYAQ
SWDDRQPNDKIDAHIKVQKLMDQINSRPNVLEKLEKVSNIMIEKKLVNLDGTSANEEN
TMKPWQMIKILMDRDLRHAMKEFKLELEKSGIQLGPEQLAPLMTVLGLEKKK"
gene complement(413882..414910)
/gene="Hem2"
/locus_tag="P301_G11446"
mRNA complement(413882..414910)
/gene="Hem2"
/locus_tag="P301_G11446"
/product="Hem2p"
CDS complement(413882..414910)
/gene="Hem2"
/locus_tag="P301_G11446"
/note="Similar to YGL040C 'Aminolevulinate dehydratase, a
homo-octameric enzyme, catalyzes the conversion of
5-aminolevulinate to porphobilinogen, the second step in
heme biosynthesis; enzymatic activity is zinc-dependent;
localizes to the cytoplasm and nucleus'"
/codon_start=1
/product="Hem2p"
/protein_id="EWG90960.1"
/translation="MHTAEFLETEPTEISSVLAGGYNHPLLRQWQSERQLTKNMLIFP
LFISDNPDDFTEIDSLPNINRIGVNRLKDYLKPLVAKGLRSVILFGVPLIPGTKDPVG
TAADDPAGPVIQGIKFIREYFPELYIICDVCLCEYTSHGHCGVLYDDGTINRERSVSR
LAAVAVNYAKAGAHCVAPSDMIDGRIRDIKRGLINANLAHKTFVLSYAAKFSGNLYGP
FRDAACSAPSNGDRKCYQLPPAGRGLARRALERDMSEGADGIIVKPSTFYLDIMRDAS
EICKDLPICAYHVSGEYAMLHAAAEKGVVDLKTIAFESHQGFLRAGARLIITYLAPEF
LDWLDEEN"
gene 417446..417559
/locus_tag="P301_G11461"
tRNA 417446..417559
/locus_tag="P301_G11461"
/product="tRNA-Leu"
/note="TL(CAA)G2"
gene 418315..419361
/locus_tag="P301_G11466"
mRNA 418315..419361
/locus_tag="P301_G11466"
/product="hypothetical protein"
CDS 418315..419361
/locus_tag="P301_G11466"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90961.1"
/translation="MTTEKTVVFVSGATGFIALHVVDDLLKTGYKVIGSGRSQEKNDG
LLKKFKSNPNLSMEIVEDIAAPNAFDKVFQKHGKEIKVVLHIASPVHFNTTDFEKDLL
IPAVNGTKSILEAIKNYAADTVEKVVITSSVAALASPGDMKDTSFVVNEESWNKDTWE
SCQANAVSAYCGSKKFAEKTAWDFLEENQSSIKFTLSTINPGFVFGPQLFADSLRNGI
NSSSAIIANLVSYKLGDNFYNYSGPFIDVRDVSKAHLLAFEKPECAGQRLFLCEDMFC
SQEALDILNEEFPQLKGKIATGEPGSGSTFLTKNCCKCDNRKTKNLLGFQFNKFRDCI
VDTASQLLEVQSKS"
gene complement(419716..421158)
/gene="Och1"
/locus_tag="P301_G11471"
mRNA complement(419716..421158)
/gene="Och1"
/locus_tag="P301_G11471"
/product="Och1p"
CDS complement(419716..421158)
/gene="Och1"
/locus_tag="P301_G11471"
/note="Similar to YGL038C Mannosyltransferase of the
cis-Golgi apparatus, initiates the polymannose outer chain
elongation of N-linked oligosaccharides of glycoproteins"
/codon_start=1
/product="Och1p"
/protein_id="EWG90962.1"
/translation="MSRKLSHLIATRKSKTIVVTVLLIYSLLTFHLSNKRLLSQFYPS
KDDFKQTLLPTTSHSQDINLKKQITVNKKKNQLHNLRDQLSFAFPYDSQAPIPQRVWQ
TWKVGADDKNFPSSFRTYQKTWSGSYSPDYQYSLISDDSIIPFLENLYAPVPIVIQAF
KLMPGNILKADFLRYLLLFARGGIYSDMDTMLLKPIDSWPSQNKSWLNNIIDLNKPIP
YKNSKPSLLSSDEISHQPGLVIGIEADPDRDDWSEWYARRIQFCQWTIQAKPGHPILR
ELILNITATTLASVQNPGVPVSEMIDPRFEEDYNVNYRHKRRHDETYKHSELKNNKNV
DGSDIMNWTGPGIFSDIIFEYMNNVLRYNSDILLINPNLNKNDEEGSESATTPAKDVD
NDTLSKSTRKFYKKISESLQSSNSMPWEFFSFLKEPVIVDDVMVLPITSFSPDVGQMG
AQSSDDKMAFVKHMFSGSWKEDADKNAGHK"
gene complement(421651..422301)
/gene="Pnc1"
/locus_tag="P301_G11481"
mRNA complement(421651..422301)
/gene="Pnc1"
/locus_tag="P301_G11481"
/product="Pnc1p"
CDS complement(421651..422301)
/gene="Pnc1"
/locus_tag="P301_G11481"
/note="Similar to YGL037C 'Nicotinamidase that converts
nicotinamide to nicotinic acid as part of the NAD(+)
salvage pathway, required for life span extension by
calorie restriction; PNC1 expression responds to all known
stimuli that extend replicative life span'"
/codon_start=1
/product="Pnc1p"
/protein_id="EWG90963.1"
/translation="MKTLIVVDMQNDFISPLGSLTVPKGEELINPISDLMQDADRDWH
RIVVTRDWHPSRHISFAKNHKDKEPYSTYTYHSPRPGDDSTQEGILWPVHCVKNTWGS
QLVDQIMDQVVTKHIKIVDKGFLTDREYYSAFHDIWNFHKTDMNKYLEKHHTDEVYIV
GVALEYCVKATAISAAELGYKTTVLLDYTRPISDDPEVINKVKEELKAHNINVVDK"
assembly_gap 422460..422787
/estimated_length=328
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 422927..425800
/locus_tag="P301_G11491"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 424475..424807
/estimated_length=333
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 425515..425750
/estimated_length=236
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(425877..427352)
/gene="Mig1"
/locus_tag="P301_G11496"
mRNA complement(425877..427352)
/gene="Mig1"
/locus_tag="P301_G11496"
/product="Mig1p"
CDS complement(425877..427352)
/gene="Mig1"
/locus_tag="P301_G11496"
/note="Similar to YGL035C 'Transcription factor involved
in glucose repression; sequence specific DNA binding
protein containing two Cys2His2 zinc finger motifs;
regulated by the SNF1 kinase and the GLC7 phosphatase'"
/codon_start=1
/product="Mig1p"
/protein_id="EWG90964.1"
/translation="MQSPYPMTQVSNVDDGSLLKESKSKSKVAAKSEAPRPHACPICH
RAFHRLEHQTRHMRIHMKRFSRSDELTRHRRIHTNSHPRGKRGRKKKVVGSPINSASS
SATSIPDSNTANFLPPLPQQHLSPLIPIAIAPKENSSRSSTRKGRKTKFEIGESGGND
PYMVSSPKTMAKIPVSVKPPPSLALNNMNYQTSSASTALSSLSDSHSGSRLKLNALSS
LQMMTPIASSAPRTVFIDGPEQKQLQQQQNSLSPRYSNTVILPRPRSLTDFQGLNNAN
PNNNGSLRAQTQSSVQLKRPSSVLSLNDLLVGQRNTNESDSDFTTGGEDEEDGLKDPS
NSSIDNLEQDYLQEQSRKKSKTSTPTTMLSRSTSGTNLHTLGYVMNQNDLHSSSSSPD
FQKELNNRLLNVQQQQQEQHTLLQSQNTSNQSQNQNQNQMMASSSSLSTTPLLLSPRV
NMINTAISTQQTPISQSDSQVQELETLPPIRSLPLPFPHMD"
gene 429915..430641
/gene="Hop2"
/locus_tag="P301_G11511"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 430525..430578
/estimated_length=54
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(430859..431122)
/gene="Aga2"
/locus_tag="P301_G11516"
mRNA complement(430859..431122)
/gene="Aga2"
/locus_tag="P301_G11516"
/product="Aga2p"
CDS complement(430859..431122)
/gene="Aga2"
/locus_tag="P301_G11516"
/note="Similar to YGL032C Adhesion subunit of a-agglutinin
of a-cells, C-terminal sequence acts as a ligand for
alpha-agglutinin (Sag1p) during agglutination, modified
with O-linked oligomannosyl chains, linked to anchorage
subunit Aga1p via two disulfide bonds"
/codon_start=1
/product="Aga2p"
/protein_id="EWG90965.1"
/translation="MQLLRCFSIFSVIASVLAQELTTICEQIPSPTLESTPYSLSTTT
ILANGKAMQGVFEYYKSVTFVSNCGSHPSTTSKGSPINTQYVF"
gene complement(431755..432222)
/gene="Rpl24a"
/locus_tag="P301_G11521"
mRNA complement(431755..432222)
/gene="Rpl24a"
/locus_tag="P301_G11521"
/product="Rpl24ap"
CDS complement(431755..432222)
/gene="Rpl24a"
/locus_tag="P301_G11521"
/note="Similar to YGL031C 'Ribosomal protein L30 of the
large (60S) ribosomal subunit, nearly identical to Rpl24Bp
and has similarity to rat L24 ribosomal protein; not
essential for translation but may be required for normal
translation rate'"
/codon_start=1
/product="Rpl24ap"
/protein_id="EWG90966.1"
/translation="MKVEIDSFSGAKIYPGRGTLFVRGDSKIFRFQNSKSASLFKQRK
NPRRIAWTVLFRKHHKKGITEEVAKKRSRKTVKAQRPITGASLDLIKERRSLKPEVRK
ANREEKLKANKEKKKAEKAARKAEKAKSAGTQSSKFSKQQAKGAFQKVAATSR"
gene 433374..433921
/gene="Rpl30"
/locus_tag="P301_G11531"
mRNA join(433374..433376,433607..433921)
/gene="Rpl30"
/locus_tag="P301_G11531"
/product="Rpl30p"
CDS join(433374..433376,433607..433921)
/gene="Rpl30"
/locus_tag="P301_G11531"
/note="Similar to YGL030W 'Protein component of the large
(60S) ribosomal subunit, has similarity to rat L30
ribosomal protein; involved in pre-rRNA processing in the
nucleolus; autoregulates splicing of its transcript'"
/codon_start=1
/product="Rpl30p"
/protein_id="EWG90967.1"
/translation="MAPVKSQESINQKLALVIKSGKYTLGYKSTVKSLRQGKSKLIII
AANTPVLRKSELEYYAMLSKTKVYYFQGGNNELGTAVGKLFRVGVVSILEAGDSDILT
TLA"
gene 434346..434708
/gene="Cgr1"
/locus_tag="P301_G11536"
mRNA 434346..434708
/gene="Cgr1"
/locus_tag="P301_G11536"
/product="Cgr1p"
CDS 434346..434708
/gene="Cgr1"
/locus_tag="P301_G11536"
/note="Similar to YGL029W 'Protein involved in nucleolar
integrity and processing of the pre-rRNA for the 60S
ribosome subunit; transcript is induced in response to
cytotoxic stress but not genotoxic stress'"
/codon_start=1
/product="Cgr1p"
/protein_id="EWG90968.1"
/translation="MVNETGESQKAAKGTPVSGKVWKAEKTPLRAKSRVVKNKKLTSW
ELKKQKRLEDKQFKERLKALKDEKEEARQAKITMLKERREKKEENERYERLAAKMHAK
KVERMRRREKRNKALKER"
gene complement(434999..435090)
/locus_tag="P301_G11541"
tRNA complement(434999..435090)
/locus_tag="P301_G11541"
/product="tRNA-Phe"
/note="TF(GAA)G"
gene complement(435569..437197)
/gene="Scw11"
/locus_tag="P301_G11546"
mRNA complement(435569..437197)
/gene="Scw11"
/locus_tag="P301_G11546"
/product="Scw11p"
CDS complement(435569..437197)
/gene="Scw11"
/locus_tag="P301_G11546"
/note="Similar to YGL028C 'Cell wall protein with
similarity to glucanases; may play a role in conjugation
during mating based on its regulation by Ste12p'"
/codon_start=1
/product="Scw11p"
/protein_id="EWG90969.1"
/translation="MISPISFLSSLLCLTYLTSALPILPKREVVTRVHTASTTNVVTD
FYSTTTEVVIAPTVEFLISDSVTFTTTLIPQGVNPTAEPTTTITKVLLKKAEMSTSSQ
PTSTLQPSTIPQSTSSFQAESTLQAVSTQQTAMYVSAGTSEDVQQLATTSTSISSSPS
PTTTSTSTQNIVSGVGEANVEGNTQSQHHSHQAAATSTLNQQTTTSIASQESTESTNT
PTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSSTSSTQETAATTSEGSSSSSAA
ITSSPKAIAYSPYNDDGSCKSADAVSSDLTLIKSKGISKIRVYGTDCNSFETVQPAAV
KLGIKINQGLYITSSGVDSIDDSVTTLIQYGQTNGWDVFDFITVGNEAINNGWCSVSD
LISKISSVKSKLSEAGYSGQITTSEPPVSFENNPDLCKKSDIDFVGINPHAYFDTSAS
AETAGTFVKGQVELIQGVCGTSNVFVTETGYPSSGIQNGGNIPSTANQITAVQNILNE
MDLDVTILSTYNDYWKAPGDYGIEQSFGVIEYFP"
gene complement(437929..440430)
/gene="Cwh41"
/locus_tag="P301_G11551"
mRNA complement(437929..440430)
/gene="Cwh41"
/locus_tag="P301_G11551"
/product="Cwh41p"
CDS complement(437929..440430)
/gene="Cwh41"
/locus_tag="P301_G11551"
/note="Similar to YGL027C 'Processing alpha glucosidase I,
ER type II integral membrane N-glycoprotein involved in
assembly of cell wall beta 1,6 glucan and
asparagine-linked protein glycosylation; also involved in
ER protein quality control and sensing of ER stress'"
/codon_start=1
/product="Cwh41p"
/protein_id="EWG90970.1"
/translation="MLISKSKMFKTFWILTSIVLLASATVDISKLQEFEEYQKFTNES
LLWAPYRSNCYFGMRPRYVHESPLIMGIMWFNSLSQDGLHSLRHFATPQDKLQKYGWE
VYDPRIGGKEVFIDEKNNLNLTVYFVKSKNGENWSVRVQGEPLDPKRPSTASVVLYFS
QNGGEIDGKSSLAMIGHDGPNDMKFFGYSKELGEYHLTVKDNFGHYFKNPEYETMEVA
PGSDCSKTSHLSLQIPDKEVWKARDVFQSLVSDSIRDILEKEETKQRPADLIPSVLTI
RNLYNFNPGNFHYIQKTFDLTKKDGFQFDITYNKLGTTQSISTREQVTELITWSLNEI
NARFDKQFSFGEGPDSIESVEVKRRFALETLSNLLGGIGYFYGNQLIDRETEFDESQF
TEIKLLNAKEEGPFELFTSVPSRGFFPRGFYWDEGFHLLQIMEYDFDLAFEILASWFE
MIEDDSGWIAREIILGNEARSKVPQEFQVQNPNIANPPTLLLAFSEMLSRAIENIGDF
NSDSYHQVMFNSRTAKFMTNNLEANPGLLTEYAKKIYPKLLKHYNWFRKSQAGLIDEY
EEILEDEGIWDKIHKNEVYRWVGRTFTHCLPSGMDDYPRAQPPDVAELNVDALAWVGV
MTRSMKQIAHVLKLTQDEQRYAQIEQEVVENLDLLHWSENDNCYCDISIDPEDDEIRE
FVCHEGYVSVLPFALKLIPKNSPKLEKVVALMSDPEKIFSDYGLLSLSRQDDYFGKDE
NYWRGPIWMNINYLCLDAMRYYYPEVILDVAGEASNAKKLYQSLKINLSNNIYKVWEE
QGYCYENYSPIDGHGTGAEHFTGWTALVVNILGRF"
gene complement(440699..442822)
/gene="Trp5"
/locus_tag="P301_G11556"
mRNA complement(440699..442822)
/gene="Trp5"
/locus_tag="P301_G11556"
/product="Trp5p"
CDS complement(440699..442822)
/gene="Trp5"
/locus_tag="P301_G11556"
/note="Similar to YGL026C 'Tryptophan synthase, catalyzes
the last step of tryptophan biosynthesis; regulated by the
general control system of amino acid biosynthesis'"
/codon_start=1
/product="Trp5p"
/protein_id="EWG90971.1"
/translation="MSEQLRQTFANAKKENRNALVTFMTAGYPTVKDTVPILKGFQDG
GVDIIELGMPFSDPIADGPTIQLSNTVALQNGVTLPQTLEMVSQARNEGVTVPIILMG
YYNPILNYGEERFIQDAAKAGANGFIIVDLPPEEALKVRNYVNDNGLSLIPLVAPSTT
DERLELLSHIADSFVYVVSRMGTTGVQSSVASDLDELISRVRKYTKDTPLAVGFGVST
REHFQSVGSVADGVVIGSKIVTLCGDAPEGKRYDVAKEYVQGILNGAKHKVLSKDEFF
AFQKESLKSANVKKEILDEFDENHKHPIRFGDFGGQYVPEALHACLRELEKGFDEAVA
DPTFWEDFKSLYSYIGRPSSLHKAERLTEHCQGAQIWLKREDLNHTGSHKINNALAQV
LLAKRLGKKNVIAETGAGQHGVATATACAKFGLTCTVFMGAEDVRRQALNVFRMRILG
AKVIAVTNGTKTLRDATSEAFRFWVTNLKTTYYVVGSAIGPHPYPTLVRTFQSVIGKE
TKEQFAAMNNGKLPDAVVACVGGGSNSTGMFSPFEHDTSVKLLGVEAGGDGVDTKFHS
ATLTAGRPGVFHGVKTYVLQDSDGQVHDTHSVSAGLDYPGVGPELAYWKSTGRAQFIA
ATDAQALLGFKLLSQLEGIIPALESSHAVYGACELAKTMKPDQHLVINISGRGDKDVQ
SVAEVLPKLGPKIGWDLRFEEDPSA"
gene complement(443052..444212)
/gene="Pgd1"
/locus_tag="P301_G11561"
mRNA complement(443052..444212)
/gene="Pgd1"
/locus_tag="P301_G11561"
/product="Pgd1p"
CDS complement(443052..444212)
/gene="Pgd1"
/locus_tag="P301_G11561"
/note="Similar to YGL025C 'Subunit of the RNA polymerase
II mediator complex; associates with core polymerase
subunits to form the RNA polymerase II holoenzyme;
essential for basal and activated transcription; direct
target of Cyc8p-Tup1p transcriptional corepressor'"
/codon_start=1
/product="Pgd1p"
/protein_id="EWG90972.1"
/translation="MDSIIPAGVKLDDLQVILAKNENETRDKVCKQINEARDEILPLR
LQFNEFIQIMANIDQEGPKQADRMAKYLHIRDKILQLNDRFQTLSSHLEALQPLFSTV
PEYLKTADNRDRSFQLLEPLSTYNKNGNAVCSTAAVVSTNHSTAASTPTTTATPHANP
ITHAHSLSNPNSTATMQHNPLAGKRGPKSGSTMGTPTVHNSTAAAPIAAPKKPRKPRQ
TKKAKAQAQAQAQVYAQQSTVQTPITASMAAALPNPTPSMINSVSPTNVMGTPLTNMM
SPMGNAYSMGAQNQGGQVSMSQFNGSGNGSNPNTNTNSNNTPLQSQLNLNNLTPANIL
NMSMNNDFQQQQQPQPQYNMNMGMNNMNNGGKELDSLDLNNLELGGLNMDFL"
gene complement(444452..446358)
/gene="Pib2"
/locus_tag="P301_G11571"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 446658..448814
/gene="Stt3"
/locus_tag="P301_G11576"
mRNA 446658..448814
/gene="Stt3"
/locus_tag="P301_G11576"
/product="Stt3p"
CDS 446658..448814
/gene="Stt3"
/locus_tag="P301_G11576"
/note="Similar to YGL022W 'Subunit of the
oligosaccharyltransferase complex of the ER lumen, which
catalyzes asparagine-linked glycosylation of newly
synthesized proteins; forms a subcomplex with Ost3p and
Ost4p and is directly involved in catalysis'"
/codon_start=1
/product="Stt3p"
/protein_id="EWG90973.1"
/translation="MGSDRSCVLSVFQTILKLVIFVAIFGAAISSRLFAVIKFESIIH
EFDPWFNYRATKYLVNNSFYKFLNWFDDRTWYPLGRVTGGTLYPGLMTTSAFIWHALR
NWLGLPIDIRNVCVLFAPLFSGVTAWATYEFTKEIKDASAGLLAAGFIAIVPGYISRS
VAGSYDNEAIAITLLMVTFMFWIKAQKTGSIMHATCAALFYFYMVSAWGGYVFITNLI
PLHVFLLILMGRYSSKLYSAYTTWYAIGTVASMQIPFVGFLPIRSNDHMAALGVFGLI
QIVAFGDFVKGQISTAKFKVIMMVSLFLILVLGVVGLSALTYMGLIAPWTGRFYSLWD
TNYAKIHIPIIASVSEHQPVSWPAFFFDTHFLIWLFPAGVFLLFLDLKDGHVFVIAYS
VLCSYFAGVMVRLMLTLTPVICVSAAVALSKIFDIYLDFKTSDRKYAIKPAALLAKLI
VSGSFIFYLYLFVFHSTWVTRTAYSSPSVVLPSQTPDGKLALIDDFREAYYWLRMNSD
EDSKVAAWWDYGYQIGGMADRTTLVDNNTWNNTHIAIVGKAMASPEEKSYEILKEHDV
DYVLVIFGGLIGFGGDDINKFLWMIRISEGIWPEEIKERDFYTAEGEYRVDARASETM
RNSLLYKMSYKDFPQLFNGGQATDRVRQQMITPLDVPPLDYFDEVFTSENWMVRIYQL
KKDDAQGRTLRDVGELTRSSTKTRRSIKRPELGLRV"
gene 449039..451307
/gene="Alk1"
/locus_tag="P301_G11581"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(451417..452124)
/gene="Get1"
/locus_tag="P301_G11586"
mRNA complement(451417..452124)
/gene="Get1"
/locus_tag="P301_G11586"
/product="Get1p"
CDS complement(451417..452124)
/gene="Get1"
/locus_tag="P301_G11586"
/note="Similar to YGL020C 'Subunit of the GET complex;
involved in insertion of proteins into the ER membrane;
required for the retrieval of HDEL proteins from the Golgi
to the ER in an ERD2 dependent fashion and for normal
mitochondrial morphology and inheritance'"
/codon_start=1
/product="Get1p"
/protein_id="EWG90974.1"
/translation="MHWAAAVAIFFIVVTKFLQYTNKYHEKWISKFAPGNELSKKYLA
KVKERHELKEFNNSISAQDNYAKWTKNNRKLDSLDKEINNLKDEIQSENKAFQAHLHK
LRLLALTVPFFVFKIMYGKTPVYKLSSSTSTLFPTFVSGVWSQGWLYVLLHPLRTISQ
KWHIMEGKFGASKFDDMALQSVSLGIWVWALMNVINGVEFIVKQLFLTPKMEAPASVE
TQEEKALDAVDDAIILD"
gene 452410..453246
/gene="Ckb1"
/locus_tag="P301_G11591"
mRNA 452410..453246
/gene="Ckb1"
/locus_tag="P301_G11591"
/product="Ckb1p"
CDS 452410..453246
/gene="Ckb1"
/locus_tag="P301_G11591"
/note="Similar to YGL019W 'Beta regulatory subunit of
casein kinase 2 (CK2), a Ser/Thr protein kinase with roles
in cell growth and proliferation; CK2, comprised of CKA1,
CKA2, CKB1 and CKB2, has many substrates including
transcription factors and all RNA polymerases'"
/codon_start=1
/product="Ckb1p"
/protein_id="EWG90975.1"
/translation="MSQEFVEDYSRTGSSDDEDSGAYDEWIPSFCSRFGHEYFCQVPT
EFIEDDFNMTSLSQEVPHYRKALDLILDLEAMSDEEEDEDDVVEEDEVDQEMQSNDGH
DEGKRRNKSPVVNKSIIEHAAEQLYGLIHARFILTKPGLQAMAEKFDHKEFGTCPRYY
CNGMQLLPCGLSDTVGKHTVRLYCPSCQDLYLPQSSRFLCLEGAFWGTSFPGVFLKHF
KELEEYVERKSKESYELKVFGFRINDEAVSGPRMKWLRQYPSTEEDWEEFAKCEFETP
AV"
gene complement(453364..453918)
/gene="Jac1"
/locus_tag="P301_G11596"
mRNA complement(453364..453918)
/gene="Jac1"
/locus_tag="P301_G11596"
/product="Jac1p"
CDS complement(453364..453918)
/gene="Jac1"
/locus_tag="P301_G11596"
/note="Similar to YGL018C 'Specialized J-protein that
functions with Hsp70 in Fe-S cluster biogenesis in
mitochondria, involved in iron metabolism; contains a J
domain typical to J-type chaperones; localizes to the
mitochondrial matrix'"
/codon_start=1
/product="Jac1p"
/protein_id="EWG90976.1"
/translation="MLKYLVQRRFTSTFYELFPKTFPKKLPIWTIDQSRLRKEYRQLQ
AQHHPDMAQQGSEQSSTLNQAYHTLKDPLRRSQYMLKLLRNIDLTQEQTSHEVTTSDP
QLLLKVLDIHDELSQMDDEAGVKLLEKQNKERIQDIEAQLGQCYNDKDYAAAVKLTVE
LKYWYNLAKAFKDWAPGKQLEMNH"
gene 454107..455618
/gene="Ate1"
/locus_tag="P301_G11601"
mRNA 454107..455618
/gene="Ate1"
/locus_tag="P301_G11601"
/product="Ate1p"
CDS 454107..455618
/gene="Ate1"
/locus_tag="P301_G11601"
/note="Similar to YGL017W Arginyl-tRNA-protein
transferase, catalyzes post-translational conjugation of
arginine to the amino termini of acceptor proteins which
are then subject to degradation via the N-end rule
pathway"
/codon_start=1
/product="Ate1p"
/protein_id="EWG90977.1"
/translation="MSDRYVIWAPSMHNEPAAKCGYCHGNKGGNMDQLFALDSWAHRY
MNKMDVVKIENCTIGSFVEHMDVATYDRMCNMGFRRSGKFLYKVDPLRNCCRLYTIRT
APQELNMTKELKKCISRFATRITSEDYCPAAVASSDFVGKIVNAEKNSKTFYTRFEPA
LYSEEKYHLFVKYQEKVHQDYNNSPKSFKRFLCDTPFGPEAVLGTQESWEQLNNWQRM
KPGEKLKHMGPVHECYYYEGKLIAITVSDILPSGISSVYFIWDPDYSKWSLGKLSALR
DLAIIQRTNLQYYYLGYYIEDCPKMNYKANYGAEVLDVCHSKYIPLKTIQDMISRGKL
FVIGEEETKVTKELYLVDSETGRGEGFPTDSVVKYKNIAEEIYGVGGCAFKSANESAL
ELKELYGIPYEEEDLDTIYHLKEHNGHAPNGIPNVVPGLLPLWELLDIMQSGKITDLE
GRLFLFEIETEGIRPLINFYSEPPNVKKRICDVIRLFGFETCMKAVILYSEQM"
gene 455919..459164
/gene="Kap122"
/locus_tag="P301_G11606"
mRNA 455919..459164
/gene="Kap122"
/locus_tag="P301_G11606"
/product="Kap122p"
CDS 455919..459164
/gene="Kap122"
/locus_tag="P301_G11606"
/note="Similar to YGL016W 'Karyopherin beta, responsible
for import of the Toa1p-Toa2p complex into the nucleus;
binds to nucleoporins Nup1p and Nup2p; may play a role in
regulation of pleiotropic drug resistance'"
/codon_start=1
/product="Kap122p"
/protein_id="EWG90978.1"
/translation="MSSIHEVVALIEELYSPHPKHDVNQIQQSLQSIQKSEQGFHLAN
ELLSDDKYSANVKYFGALTLTVQLNTRGENDYETLWNVFRSNLLYLTKFSTLYVSNPN
MYGQSLIIIKKLMSNLSLIFTKINDPQLNNAGNENMIKQWNNPINTFIQLMSVQNQNI
NADQLLLDSINCSLTYEQLSQFVSLSQKHNELALTFTEVIVEDLTKFQTKRHSMSQIH
EVVHEHLYISTMALINLNLTAQAVFNPTVFDCITAWINYISLTRSVSSSGRMDLSEIF
QNLIDLMYQSTEGSDGYENAEKILTIFGNVFANDPLLMSYDLRQQIECIFLGVVRPDS
GITXXXXXXXXXXXXXXXXXXXXXXXXXXXLAICIVDFLQINTLSVCNKLFTNIQTAD
NGQVQDEYIQEYIKVLLQMTNFPLTPVLQEFFSVRMVDFWLDLSDAYTNLASETLRPN
SIELSTQIFQQLINIYLPKISLSVKQRIIEEEGESTSVNEFEDFRNAVSDLAQSLWSI
LGNDNLTNVLIDGMGQMPAASDETLIIKDTDVLFRIETMCFVLNTILVDMTLSESPWI
KNIVDANKFFNQNVISVFQTGFQTSASTKVSQILKLDFVRTSTTLIGTLAGYFKQEPF
QLNPYVEALFQGLHTCTNFTSKNEQEKISNDKLEVMVIKTVSTLCETCREELTPYLMH
FISFLNTVIMPDSNVSHFTRTKLVRSIGYVVQCQVSNGPEEQAKYILQLTNLLSGSIE
HCLASSVQLQEQQDYINCLLYCISELATSLIQPTEIIENDALLQRLSEFQSFWSSDPL
QIRSKIMCTIDKVLDNSIYCKNSAFVEIGCLIVGKGLNLPDGEPYFLKYNMSEVMNFV
LRHVPNCELATCLPYFVYLLEKLISEFRKELTPQEFDFMFEKILLVYYDAYIINDPDL
LQMTIGFVNNVLDVKPGLAIGSKHWTSFILPQFLKLIPSREKFTIVAVAKFWTKLINN
KKYNQEELTTVRQQVSSIGGDLVYQIMYGLFHTQRSDLNSYTDLLRALVAKFPIEARE
WLVAVLPQICNNPAGHEKFINKLLITRGSRAAGNVILQWWLDCTTLPNYQG"
assembly_gap 456931..457009
/estimated_length=79
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(459296..459688)
/locus_tag="P301_G11611"
mRNA complement(459296..459688)
/locus_tag="P301_G11611"
/product="hypothetical protein"
CDS complement(459296..459688)
/locus_tag="P301_G11611"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90979.1"
/translation="MEDTIRPLNYADIETSGPINLLETTNNLKSSLKKFSQKAKGSHI
SRERIHHFRKWKNKTESLSENHLKPPPDVDSLCFSNCFQPDALSGNVFLPPRSSNMYW
NEKQLQLEMEILKFLSLNTSNECCTSDD"
gene 460389..463082
/gene="Puf4"
/locus_tag="P301_G11616"
mRNA 460389..463082
/gene="Puf4"
/locus_tag="P301_G11616"
/product="Puf4p"
CDS 460389..463082
/gene="Puf4"
/locus_tag="P301_G11616"
/note="Similar to YGL014W 'Member of the PUF protein
family, which is defined by the presence of Pumilio
homology domains that confer RNA binding activity;
preferentially binds mRNAs encoding nucleolar ribosomal
RNA-processing factors'"
/codon_start=1
/product="Puf4p"
/protein_id="EWG90980.1"
/translation="MSTKGLKEEIDDVPSVDPVVSETVNSALEQLQLDDPEENATSNA
FANKVSQDSQFANGPPSQMFPHPQMMGGMGFMPYSQMMXXXHNPCPFFPPPDFNDPTA
PLSSSPLNAGGPPMLFKNDSLPFQMLSSGAAVATQGGQNLNPLINDNSMKVLPIASAD
PLWTHSNVPGSASVAIEETTATLQESLPSKGRESNNKASSFRRQTFHALSPTDLINAA
NNVTLSKDFQSDMQNFSKAKKPSVGANNTAKTRTQSISFDNTPSSTSFIPPTNSVSEK
LSDFKIEETSKEDLINKTAPAKKESPTTYGAAYPYGGPLLQPNPIMPGHPHNISSPIY
GIRSPFPNSYEMGAQFQPFSPILNPTSHSLNANSPIPLTQSPIHLAPVLNPSSNSVAF
SDMKNDGGKPTTDNDKAGPNVRMDSINPNLGPSMQPFHILPPQQNTPPPPWLYGTPPP
FNAMVPPHLLAQNHMPLMNSANNKHHGRNNNSMSSHNDNDNIGNSNYNNKDTGRSNVG
KMKNMKNSYHGYYNNNNNNNNNNNNNNNNNNNNNNNSNATNSNSAEKQRKIEESSRFA
DAVLDQYIGSIHSLCKDQHGCRFLQKQLDILGSKAADAIFEETKDYTVELMTDSFGNY
LIQKLLEEVTTEQRIVLTKISSPHFVEISLNPHGTRALQKLIECIKTDEEAQIVVDSL
RPYTVQLSKDLNGNHVIQKCLQRLKPENFQFIFDAISDSCIDIATHRHGCCVLQRCLD
HGTTEQCDNLCDKLLALVDKLTLDPFGNYVVQYIITKEAEKNKYDYTHKIVHLLKPRA
IELSIHKFGSNVIEKILKTAIVSEPMILEILNNGGETGIQSLLNDSYGNYVLQTALDI
SHKQNDYLYRRLSEIVAPLLVGPIRNTPHGKRIIGMLHLDS"
assembly_gap 463119..463292
/estimated_length=174
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(463322..466552)
/gene="Pdr1"
/locus_tag="P301_G11626"
mRNA complement(463322..466552)
/gene="Pdr1"
/locus_tag="P301_G11626"
/product="Pdr1p"
CDS complement(463322..466552)
/gene="Pdr1"
/locus_tag="P301_G11626"
/note="Similar to YGL013C Zinc cluster protein that is a
master regulator involved in recruiting other zinc cluster
proteins to pleiotropic drug response elements (PDREs) to
fine tune the regulation of multidrug resistance genes"
/codon_start=1
/product="Pdr1p"
/protein_id="EWG90981.1"
/translation="MRGLTPKNGVHIETGPDTESSADSSNFSTGFSGKIRKPRSKVSK
ACDNCRKRKIKCNGKFPCASCEIYSCECTFSTRQGGAQIKNLHKTSLEGATVQVKEET
DSSSTSFSNPQRCTDGPCAVEQPTKFFENFKLGGRSSGDNSGSDGKNDDDVNRNGFYE
DDSESQATLTSLQTTLKNLKEMAHLGTHVTSAIESIELQISDLLKRWEPKVRTKELAT
TKFYPNKSIETQLMKNKYCDVVHLTRYAAWSNNKKDQDTSSQPLIDEIFGLYSHFQFL
SLQGIGKCFQNYRSKSKCEIFPRTAKETIYIMLRFFDVCFHHINQGCVSIANPLENYL
QKMNLLPSTPSSISSAGSPNTAHTKSHVALVINHLPQPFVRNITGISNSELLSEMNND
ISMFGILLKMLDMHKNSYQNFLMEITSNPSVAKNTQSIDVLQEFIHYCQAGEALIALC
YSYYNSTLYNYVDFTCDITHLEQLLYFLDLLFWLSEIYGFEKVLNVAVHFVSRVGLSR
WEFYVGLDENFAERRRNLWWKAFYFEKTLASKLGYPSNIDDSKINCLLPKNFRDVGFL
DNRDFIENVHLVRRSEAFDNMCISDLKYYGELAVLQIVSHFSSSVLFNEKFTSIRNTS
KPSVVREKLLFEVLEIFNETEMKYDAIKEQTGKLFDIAFSKDSTELKVSREDKIMASK
FVLFYEHHFCRMVNESDNIVARLCVHRRPSILIENLKIYLHKIYKSWTDMNKILLDFD
NDYSVYRSFAHYSISCIILVSQAFSVAEFIKVNDVVNMIRVFKRFLDIKIFSENETNE
HVFNSQSFKDYTRAFSFLTIVTRIMLLAYGESSSTNLDVISKYIDENAPDLKGIIELV
LDTNSCAYRFLLEPVQKSGFHLTVSQMLKNRKFQEPLMSNEDNKQMKHNSGKNLNPDL
PSLKTGTSCLLNGIESPQLPFNGRSAPSPVRNNSLPEFAQLPSFRSLSVSDMINPDYA
QPTNGQNNTQVQSNKPINAQQQIPTSVQVPFMNTNEINNNNNNNNNNNNNNNNNNKNN
INNINNNNSNNFSATSFNLGTLDEFVNNGDLEDLYSILWSDVYPDS"
gene 467109..468530
/gene="Erg4"
/locus_tag="P301_G11631"
mRNA 467109..468530
/gene="Erg4"
/locus_tag="P301_G11631"
/product="Erg4p"
CDS 467109..468530
/gene="Erg4"
/locus_tag="P301_G11631"
/note="Similar to YGL012W 'C-24(28) sterol reductase,
catalyzes the final step in ergosterol biosynthesis;
mutants are viable, but lack ergosterol'"
/codon_start=1
/product="Erg4p"
/protein_id="EWG90982.1"
/translation="MAKDNSEKLQVQGEEKKSKQPVNFLPQGKWLKPNEIEYEFGGTT
GVIGMLIGFPLLMYYMWICAEFYHGKVALPKAGESWMHFIKHLYQLVLENGIPEKYDW
TIFLTFWVFQIIFYYTLPGIWTKGQPLSHLKGKQLPYFCNAMWTLYVTTTLVLVLHFT
NLFRLYVIIDRFGRIMTCAIISGFAFSIILYLWTLFISHDYHRMTGNHLYDFFMGAPL
NPRWGILDLKMFFEVRLPWFTLYFITLGACLKQWETYGYVTPQLGVVMLAHWLYANAC
AKGEELIVPTWDMAYEKFGFMLIFWNIAGVPYTYCHCTLYLYYHDPSEYHWSTLYNVS
LYVVLLCAYYFFDTANAQKNAFRKQMSGDKTVRKTFPFLPYQILKNPKYMVTSNGSYL
LIDGWYTLARKIHYTADWTQSLVWALSCGFNSVLPWFFPVFFLVVLIHRAFRDQAKCK
RKYGKDWDEYCKHCPYVFIPYVF"
gene complement(468743..469501)
/gene="Scl1"
/locus_tag="P301_G11636"
mRNA complement(468743..469501)
/gene="Scl1"
/locus_tag="P301_G11636"
/product="Scl1p"
CDS complement(468743..469501)
/gene="Scl1"
/locus_tag="P301_G11636"
/note="Similar to YGL011C 'Alpha 1 subunit of the 20S
proteasome involved in the degradation of ubiquitinated
substrates; 20S proteasome is the core complex of the 26S
proteasome; essential for growth; detected in the
mitochondria'"
/codon_start=1
/product="Scl1p"
/protein_id="EWG90983.1"
/translation="MSGAAAASAAGYDRHITIFSPEGRLYQVEYAFKATNQTNINSLA
VRGKDCTVVISQKKVPDKLLDPTTVSYIFCISRTIGMVVNGPIPDARNAALRAKAEAA
EFRYKYGYDMPCDVLAKRMANLSQIYTQRAYMRPLGVILTFVSVDEELGPSIYKTDPA
GYYVGYKATATGPKQQEITTNLENHFKKSKIDHINEESWEKVVEFAITHMIDALGTEF
SKNDLEVGVATKDKFFTLSAENIEERLVAIAEQD"
gene 469799..470323
/locus_tag="P301_G11641"
mRNA 469799..470323
/locus_tag="P301_G11641"
/product="hypothetical protein"
CDS 469799..470323
/locus_tag="P301_G11641"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90984.1"
/translation="MGEGLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLH
RVKIYQSISLTAVLSVLFSIFYCLLYLPTGLLAGVLLLLLNLALIDHRVDLTFKQELG
LFIIGWIFQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKLGFMPRLKATLEH
DLEIKQRNLRMQRQ"
gene complement(470567..472906)
/gene="Leu1"
/locus_tag="P301_G11646"
mRNA complement(470567..472906)
/gene="Leu1"
/locus_tag="P301_G11646"
/product="Leu1p"
CDS complement(470567..472906)
/gene="Leu1"
/locus_tag="P301_G11646"
/note="Similar to YGL009C Isopropylmalate isomerase,
catalyzes the second step in the leucine biosynthesis
pathway"
/codon_start=1
/product="Leu1p"
/protein_id="EWG90985.1"
/translation="MVYTPSKGPRTLYDKVFDAHVVHQDENGSFLLYIDRHLVHEVTS
PQAFEGLENAGRKVRRVDCTLATVDHNIPTESRKNFKSLDTFIKQTDSRLQVKTLENN
VKQFGVPYFGMSEARQGIVHTIGPEEGFTLPGTTVVCGDSHTSTHGAFGSLAFGIGTS
EVEHVLATQTIIQAKSKNMRITVNGKLSPGITSKDLILYIIGLIGTAGGTGCVIEFAG
EAIEALSMEARMSMCNMAIEAGARAGMIKPDETTFQYTKGRPLAPKGTEWEKAVAYWK
TLKTDEGAKFDHEINIEAVDVIPTITWGTSPQDALPITGSVPDPKNVTEPIKKSGMER
ALAYMGLEPNTPLKSIKVDKVFIGSCTNGRIEDLRSAAAVVRGQKLASNIKLAMVVPG
SGLVKKQAEAEGLDKIFQEAGFEWREAGCSMCLGMNPDILDAYERCASTSNRNFEGRQ
GALSRTHLMSPAMAAAAGIAGHFVDIREFEYKDQDQSSPKVEVTSEDEKELESAAYDH
AEPVQPEDAPQDIANDELKDIPVKSDDTPAKPSSSGMKPFLTLEGISAPLDKANVDTD
AIIPKQFLKTIKRTGLKKGLFYEWRFRKDDQGKDQETDFVLNVEPWREAEILVVTGDN
FGCGSSREHAPWALKDFGIKSIIAPSYGDIFYNNSFKNGLLPIRLDQQIIIDKLIPIA
NKGGKLCVDLPNQKILDSDGNVLVDHFEIEPFRKHCLVNGLDDIGITLQKEEYISRYE
ALRREKYSFLEGGSKLLKFDNVPKRKAVTTTFDKVHQDW"
assembly_gap 473661..473966
/estimated_length=306
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(474123..476879)
/gene="Pma1"
/locus_tag="P301_G11651"
mRNA complement(474123..476879)
/gene="Pma1"
/locus_tag="P301_G11651"
/product="Pma1p"
CDS complement(474123..476879)
/gene="Pma1"
/locus_tag="P301_G11651"
/note="Similar to YGL008C 'Plasma membrane H+-ATPase,
pumps protons out of the cell; major regulator of
cytoplasmic pH and plasma membrane potential; P2-type
ATPase; Hsp30p plays a role in Pma1p regulation;
interactions with Std1p appear to propagate [GAR+]'"
/codon_start=1
/product="Pma1p"
/protein_id="EWG90986.1"
/translation="MTDTSSSSSSSSASSVSAHQPTQEKPAKTYDDAASESSDDDDID
ALIEELQSNHGVDDEDSDNDGPVAAGEARLVPEEYLQTDPSYGLTSDEVLKRRKKYGL
NQMADEKESLVVKFVMFFVGPIQFVMEAAAILAAGLSDWVDFGVICGLLMLNAGVGFV
QEFQAGSIVDELKKTLANTAVVIRDGQLVEIPANEVVPGDILQLEDGTIIPTDGRIVT
EDCFLQIDQSAITGESLAVDKHYGDQTFSSSTVKRGEGFMVVTATGDNTFVGRAAALV
NKAAGGQGHFTEVLNGIGIILLVLVIATLLLVWTACFYRTNGIVRILRYTLGITIIGV
PVGLPAVVTTTMAVGAAYLAKKQAIVQKLSAIESLAGVEILCSDKTGTLTKNKLSLHE
PYTVEGVSPDDLMLTACLAASRKKKGLDAIDKAFLKSLKQYPKAKDALTKYKVLEFHP
FDPVSKKVTAVVESPEGERIVCVKGAPLFVLKTVEEDHPIPEDVHENYENKVAELASR
GFRALGVARKRGEGHWEILGVMPCMDPPRDDTAQTVSEARHLGLRVKMLTGDAVGIAK
ETCRQLGLGTNIYNAERLGLGGGGDMPGSELADFVENADGFAEVFPQHKYRVVEILQN
RGYLVAMTGDGVNDAPSLKKADTGIAVEGATDAARSAADIVFLAPGLSAIIDALKTSR
QIFHRMYSYVVYRIALSLHLEIFLGLWIAILDNSLDIDLIVFIAIFADVATLAIAYDN
APYSPKPVKWNLPRLWGMSIILGIVLAIGSWITLTTMFLPKGGIIQNFGAMNGIMFLQ
ISLTENWLIFITRAAGPFWSSIPSWQLAGAVFAVDIISTMFTLFGWWSENWTDIVTVV
RVWIWSIGIFCVLGGFYYEMSTSEAFDRLMNGKPMKEKKSTRSVEDFMAAMQRVSTQH
EKET"
gene complement(477153..477239)
/locus_tag="P301_G11656"
mRNA complement(477153..477239)
/locus_tag="P301_G11656"
/product="hypothetical protein"
CDS complement(477153..477239)
/locus_tag="P301_G11656"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90987.1"
/translation="MLPSISFDYIKRPNIVLFSNVLSLSSNI"
gene 479675..479902
/locus_tag="P301_G11676"
mRNA 479675..479902
/locus_tag="P301_G11676"
/product="hypothetical protein"
CDS 479675..479902
/locus_tag="P301_G11676"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG90988.1"
/translation="MGAFQKHSNSISPPLPPQGKALFXXXXXXXXXXXXXXXXXIITK
INCRLNEKLNNLFRFFSTSPNYILVRLARFN"
assembly_gap 479744..479793
/estimated_length=50
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 480152..483671
/gene="Pmc1"
/locus_tag="P301_G11681"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(483937..484776)
/gene="Cog7"
/locus_tag="P301_G11686"
mRNA complement(483937..484776)
/gene="Cog7"
/locus_tag="P301_G11686"
/product="Cog7p"
CDS complement(483937..484776)
/gene="Cog7"
/locus_tag="P301_G11686"
/note="Similar to YGL005C Component of the conserved
oligomeric Golgi complex (Cog1p through Cog8p), a
cytosolic tethering complex that functions in protein
trafficking to mediate fusion of transport vesicles to
Golgi compartments"
/codon_start=1
/product="Cog7p"
/protein_id="EWG90989.1"
/translation="MVDLTITGDDDDILSMFFDEEFVPHAFVDILLSNALNEDQIQTQ
SVSSLLLTRLDFYTKNLTKELESTIWNLDKLSQTLPRTWTSSRYHKEAEQNDSSLYST
ESLKSSKLEYYLDTLASAVRALETGMHNVTEKLSDLDNENNHNTNVRQQLQSLMLIKE
RIEKVVYYLEQVRTVTNISTVGENNTTSTGTDLSITDFRTSLKALEDTIDESLSSAID
NEAKDETNKDLIGRIDSLSELKCLFKGLDKFFAEYSNFSESIKSKAQSYLSTKDIDDG
MIS"
gene complement(484932..486185)
/gene="Rpn14"
/locus_tag="P301_G11691"
mRNA complement(484932..486185)
/gene="Rpn14"
/locus_tag="P301_G11691"
/product="Rpn14p"
CDS complement(484932..486185)
/gene="Rpn14"
/locus_tag="P301_G11691"
/note="Similar to YGL004C 'Proteasome-interacting protein
involved in the assembly of the base subcomplex of the 19S
proteasome regulatory particle (RP); null mutants
accumulate ubiquitinated Gcn4p and display decreased 26S
proteasome stability; interacts with Rpt5p'"
/codon_start=1
/product="Rpn14p"
/protein_id="EWG90990.1"
/translation="MTKTITVAHIQYDFKAVLEENDENDDEFYINVDKNLNEIKEHKI
VALGNSRGVDAGKGNTFEKVGSHLYKARLDGHDFLFNTIIRDCSKMLKRADYTAVDTA
KLQMRRFILGTTEGDIKVLDSNFNLQREIDQAHVSEITKLKFFPSGEALISSSQDMQL
KIWSVKDGSNPRTLIGHRATVTDIAIIDRGRNVLSASLDGTICLWECGTGTTIHTFNR
KENPHDGVNSIALFEGTERQLHEISTSKKNNLEFGTYGKYVIAGHVSGVITVHNVFSK
EQTIQLPSKFTCSCNSLTVDGNNANYIYAGYENGMLAQWDLRSPECPVGEFLINEGTP
INNVYFAAGALFVSSGFDTSIKLDIISDPESERPAIEFETPTFLVSNDDEVSQFCYLS
DDESNGEVLEVGKNNFCALYNLSNP"
gene complement(486703..488403)
/gene="Cdh1"
/locus_tag="P301_G11696"
mRNA complement(486703..488403)
/gene="Cdh1"
/locus_tag="P301_G11696"
/product="Cdh1p"
CDS complement(486703..488403)
/gene="Cdh1"
/locus_tag="P301_G11696"
/note="Similar to YGL003C 'Cell-cycle regulated activator
of the anaphase-promoting complex/cyclosome (APC/C), which
directs ubiquitination of cyclins resulting in mitotic
exit; targets the APC/C to specific substrates including
Cdc20p, Ase1p, Cin8p and Fin1p'"
/codon_start=1
/product="Cdh1p"
/protein_id="EWG90991.1"
/translation="MSTNLNPFMNNTPSSSPLKGSESKRVSKRPISSSSSASLLSSPS
RRSRPSTVYGDRYIPSRTDIDFNSIVSISSMTSVPALNPSSTEDQVEYQKERQAHETY
NTLLKNELFGEMLSKDTVGSESSIDRIKNTRPSTRGNVHTENTTRHGYELERVSTPPP
EAAGLEEFSPHSTPVTPRRLFTSQQDEITRPSSNSVRGASLLTYQQRKGRRLSAASLL
QSQFFDSMSPVRPDSKQLLLSPGKQFRQIAKVPYRVLDAPSLADDFYYSLIDWSSTDV
LAVALGKSIFLTDNNTGDVVHLCDTENEYTSLSWIGAGSHLAVGQANGLVEIYDVMKR
KCIRTLSGHIDRVACLSWNNHVLTSGSRDHRILHRDVRMPDPFFETIESHTQEVCGLK
WNVADNKLASGGNDNVVHVYEGTSKSPILTFDEHKAAVKAMAWSPHKRGVLATGGGTA
DRRLKIWNVNTSIKMSDIDSGSQICNMVWSKNTNELVTSHGYSKYNLTLWDCNSMDPI
AILKGHSFRVLHLTLSNDGTTVVSGAGDETLRYWKLFDKSKAKVQPNSLIFDAFNQIR
"
gene 488746..489396
/gene="Erp6"
/locus_tag="P301_G11701"
mRNA 488746..489396
/gene="Erp6"
/locus_tag="P301_G11701"
/product="Erp6p"
CDS 488746..489396
/gene="Erp6"
/locus_tag="P301_G11701"
/note="Similar to YGL002W 'Protein with similarity to
Emp24p and Erv25p, member of the p24 family involved in ER
to Golgi transport; the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Erp6p"
/protein_id="EWG90992.1"
/translation="MLSHYIFLAFVLLPFRVSAFYFYGYGGDRKCFLKELSKDTLLKG
SYNLEVYDDKLADYALPSYNDYCIVIDVEEVFDNNHRVVHQQGSPSGDFSFLALESGE
YKICLQSRVNNWVGKTKTKLEIEFEVGFEAMLDMQRKETLESLHGKVSILNSKIVDIR
REQQLMREREESFRDISESVNSRAMWWTVTQVTLLIIICVWQMKSLRSFFVKQKVL"
gene complement(489682..490731)
/gene="Erg26"
/locus_tag="P301_G11706"
mRNA complement(489682..490731)
/gene="Erg26"
/locus_tag="P301_G11706"
/product="Erg26p"
CDS complement(489682..490731)
/gene="Erg26"
/locus_tag="P301_G11706"
/note="Similar to YGL001C C-3 sterol dehydrogenase,
catalyzes the second of three steps required to remove two
C-4 methyl groups from an intermediate in ergosterol
biosynthesis"
/codon_start=1
/product="Erg26p"
/protein_id="EWG90993.1"
/translation="MSKIDSVLIIGGSGFLGLHLIQQFFDINPKPDIHIFDVRDLPEK
LSKQFTFNVDDIKFHKGDLTSPDDMENAINESKANVVVHCASPMHGQNPDIYDIVNVK
GTRNVIDMCKKCGVNILVYTSSAGVIFNGQDVHNADETWPIPEVPMDAYNETKAIAED
MVLKANDPSSDFYTVALRPAGIFGPGDRQLVPGLRQVAKLGQSKFQIGDNNNLFDWTY
AGNVADAHVLAAQKLLDPKTRTAVSGETFFITNDTPTYFWALARTVWKADGHIDKHVI
VLKRPVAICAGYLSEWVSKMLGKEPGLTPFRVKIVCAYRYHNIAKAKKLLGYTPRVGI
EEGINKTLAWMDEGL"
gene complement(491001..492256)
/gene="Aml1"
/locus_tag="P301_G11711"
mRNA complement(join(491001..491593,491687..492165,
492228..492256))
/gene="Aml1"
/locus_tag="P301_G11711"
/product="Aml1p"
CDS complement(join(491001..491593,491687..492165,
492228..492256))
/gene="Aml1"
/locus_tag="P301_G11711"
/note="Similar to YGR001C 'Putative protein of unknown
function with similarity to methyltransferase family
members; green fluorescent protein (GFP)-fusion protein
localizes to the cytoplasm; required for replication of
Brome mosaic virus in S. cerevisiae'"
/codon_start=1
/product="Aml1p"
/protein_id="EWG90994.1"
/translation="MSDSDSDYELTLSANALAALEEFKREEQQHQEAFQKLYDETDED
FQKKKKEEGMKLFKEDWQLSQFWYSDDTASILADAILEGADENTVIAIVSAPSVYAAI
QKKPTNEIPTEHIYLFEFDKRFELLAGRDHFFFYDYNKPLDFSDEIKGKVDRLLIDPP
FLNEDCQTKSSITAKCLLAPNDNSKTKKGVFKHRLISCTGERMSEVISKVFSDTRITT
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXVDLRFNES"
assembly_gap 491028..491448
/estimated_length=421
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(492697..494126)
/gene="Swc4"
/locus_tag="P301_G11716"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 494352..496589
/gene="Cul3"
/locus_tag="P301_G11721"
mRNA 494352..496589
/gene="Cul3"
/locus_tag="P301_G11721"
/product="Cul3p"
CDS 494352..496589
/gene="Cul3"
/locus_tag="P301_G11721"
/note="Similar to YGR003W 'Ubiquitin-protein ligase, forms
a complex with Elc1p that polyubiquitylates
monoubiquitylated RNA polymerase II to trigger its
proteolysis; cullin family member with similarity to
Cdc53p and human CUL3'"
/codon_start=1
/product="Cul3p"
/protein_id="EWG90995.1"
/translation="MITNKKIKISVPEKLGLSGESFEESWETVKYAVDHIYSDDMAEL
SFEQVYKTIYTIVLNKKGPILYNRLKDYLIQKLALLRETIVKDNTHDYEFLGTMARLW
DVECHCFKITGDLMMYMDKVYCKPNRCMEVYDMCLDLFRIEILQKCSSSLISALISDI
ERIRNLGSVDSEHTSLWKVLIGMMETLHDNRDSFFLTDFEPVLITATEEYYNKAIDIE
LLTPIESLEKIRKLRQFESMLDSSFLNVDSLNKLKTVLENVLIWGKLSDIIEDLTHEA
MVISNGKLLQEIYDLSNEEKYRVTVIESIKSYINKNAINIPFNGSNRKKGQNAITXXX
XXXXXYRSQLLVFWKVFDFGSVRLNNLTGDVSNAILGDVFSMYFSKEGALPSEYLSTY
VDHCMKRTKEKDAEIVKIKQDLLDSTKLIGLLTEKDIFEKIYKKQLSRRLLQQRSLVE
IEKWMVQMIKKVLGTFFTSKLEIMLRDISLSSEMYQAFQHSTTNSIEYLSFAPQVLTR
TSWPFQSTNPIDEGISLPPRMSQILAGFEGYYSLKYKERVLKWAHHLSVIEIGCQFNS
GYYEISFSVYAGVIFLLFEDYEELTLGEIYELTHIPIDDVKSLVMSMSTIPRCKILKK
SSSSGNMKFSVNYFFSSPNRKVKVPVIACPLPSQKSDNLATASSVDTYDNEIVMELSA
IIVRIMKTEGKLSHQQLLERTTKRTQSRLSLTPSIFKRSIQLLIEKEYIQRNADDPSY
YHYLS"
assembly_gap 495346..495367
/estimated_length=22
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 497161..498549
/gene="Pex31"
/locus_tag="P301_G11731"
mRNA 497161..498549
/gene="Pex31"
/locus_tag="P301_G11731"
/product="Pex31p"
CDS 497161..498549
/gene="Pex31"
/locus_tag="P301_G11731"
/note="Similar to YGR004W 'Peroxisomal integral membrane
protein, involved in negative regulation of peroxisome
size; partially functionally redundant with Pex30p and
Pex32p; probably acts at a step downstream of steps
mediated by Pex28p and Pex29p'"
/codon_start=1
/product="Pex31p"
/protein_id="EWG90996.1"
/translation="MSEINNENLEPTPSTVAESTESKNKHIRSALRKRRGKLSAQTYE
EDQEAILSSPLLTSTPKTVSRSLVRLYPYLIVVDNFLSIITWSNDNVSANLLGIFLFT
VCVLYFGFITRYFGHLMIVGIIWVYLLIDKHVQETMASCPSLDDIIHVMDRVSMKSSA
VLSPITILSAQDVRRLLFTIAFLSPVYIFLTVFVLSPNYLMLIGGLYVLTYHSKLIRR
MRRYLWKFRVVRLLVFFITGLDLGGPDNNRRLFASVNKKIRSFVWNEVGNTSNTKKTV
LFKVALFENQRRWLGIGWTSTMLSYERASWTDEFLNTSPSPEVFTLPEEQSGMAWEWH
DKDWMLDLTNDGIIQLPASAAKTKVKPGADEGFIYYDNTWNNPSATDTYKKYTRRRRW
IRTATVTTTYDDEPTVEKPTPNSHALKSEENNRVRKRKVSFSTANEVHIIPSSDSSKL
IQISDVSMSPSL"
gene complement(498880..500082)
/gene="Tfg2"
/locus_tag="P301_G11736"
mRNA complement(498880..500082)
/gene="Tfg2"
/locus_tag="P301_G11736"
/product="Tfg2p"
CDS complement(498880..500082)
/gene="Tfg2"
/locus_tag="P301_G11736"
/note="Similar to YGR005C 'TFIIF (Transcription Factor II)
middle subunit; involved in both transcription initiation
and elongation of RNA polymerase II; homologous to human
RAP30'"
/codon_start=1
/product="Tfg2p"
/protein_id="EWG90997.1"
/translation="MSSGSAGAPALSNNSTNSVAKEKSGNISGDEYLSQEEEVFDGND
IENNETKVYEESLDLDLERSNRQVWLVRLPMFLAEKWRDRNNLHGQELGKIRINKDGS
KITLLLNENDNDSIPHEYDLELTKKVVENEYVFTEQNLKKYQQRKKELEADPEKQRQA
YLKKQEREEELKKKQQQQKRRNNRKKFNHRVMTDRDGRDRYIPYVKTIPKKTAIVGTV
CHECQVMPSMNDPNYHKIVEQRRNIVKLNNKERITTLDETVGVTMSHTGMSMRSDNSN
FLKVGREKAKSNIKSIRMPKKEILDYLFKLFDEYDYWSLKGLKERTRQPEAHLKECLD
KVATLVKKGPYAFKYTLRPEYKKLKEEERKATLGELADEQTGSAGDNAQGDAEADLED
EIEMEDVV"
gene 500293..501048
/gene="Prp18"
/locus_tag="P301_G11741"
mRNA 500293..501048
/gene="Prp18"
/locus_tag="P301_G11741"
/product="Prp18p"
CDS 500293..501048
/gene="Prp18"
/locus_tag="P301_G11741"
/note="Similar to YGR006W Splicing factor involved in the
positioning of the 3' splice site during the second
catalytic step of splicing, part of snRNP U5, interacts
with Slu7p"
/codon_start=1
/product="Prp18p"
/protein_id="EWG90998.1"
/translation="MDLDLASILKGEISKKKKELANSKGVQPPCTEKFQPHESANIDE
TPRQVEQESTDEENLSDNQSDDIRTTISKLENRPERIQEAIAQDKTISVIIDPSQIGS
TEGKPLLSMKCNLYIHEILSRWKASLEAYHPELFLDTKKALFPLLLQLRRNQLAPDLL
ISLATVLYHLQQPKEINLAVQSYMKLSIGNVAWPIGVTSVGIHARSAHSKIQGGRNAA
NIMIDERTRLWITSIKRLITFEEWYTSNHDSLA"
gene 501192..502163
/gene="Muq1"
/locus_tag="P301_G11746"
mRNA 501192..502163
/gene="Muq1"
/locus_tag="P301_G11746"
/product="Muq1p"
CDS 501192..502163
/gene="Muq1"
/locus_tag="P301_G11746"
/note="Similar to YGR007W 'Ethanolamine-phosphate
cytidylyltransferase, catalyzes the second step of
phosphatidylethanolamine biosynthesis; involved in the
maintenance of plasma membrane; similar to mammalian CTP:
phosphocholine cytidylyl-transferases'"
/codon_start=1
/product="Muq1p"
/protein_id="EWG90999.1"
/translation="MTVNLDPDKVWIDGCFDFTHHGHAGAILQARRTVSKENGQLFCG
VHTDEDIQHNKGTPVMNSSERYEHTRSNRWCSEVVEAAPYVTDPNWMDKYQCQYVVHG
DDITLDANGEDCYKLVKEMGRFKVVKRTYGVSTTEIIHRILTKKSLPPTHPDYYPTTQ
ELSFYSVAQDAVSKHCYVFQRDLDNVLVNGGYKFDAEDCVYVDGDFDLFHMGDIDQLR
KLKMDLHPDKKLIVGITTSDYSSTIMTMKERVLSVLSCKYVDAVIIDADATSMSQYNC
EKYHIGTAVLTAAGKFSEYLTKELIVKRVESQREVYIARNQKKGMSI"
gene complement(502333..502587)
/gene="Stf2"
/locus_tag="P301_G11751"
mRNA complement(502333..502587)
/gene="Stf2"
/locus_tag="P301_G11751"
/product="Stf2p"
CDS complement(502333..502587)
/gene="Stf2"
/locus_tag="P301_G11751"
/note="Similar to YGR008C 'Protein involved in regulation
of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p
may act as stabilizing factors that enhance inhibitory
action of the Inh1p protein'"
/codon_start=1
/product="Stf2p"
/protein_id="EWG91000.1"
/translation="MTRTNKWTEREGKADPKYFSHTGNYGESPNHIKKQGSGKGNWGK
PGDEIDDLIDSGEIPPMFKKDRRGSNLQSHEQKFENVQKE"
gene complement(503267..505228)
/gene="Sec9"
/locus_tag="P301_G11756"
mRNA complement(503267..505228)
/gene="Sec9"
/locus_tag="P301_G11756"
/product="Sec9p"
CDS complement(503267..505228)
/gene="Sec9"
/locus_tag="P301_G11756"
/note="Similar to YGR009C 't-SNARE protein important for
fusion of secretory vesicles with the plasma membrane;
similar to but not functionally redundant with Spo20p;
SNAP-25 homolog'"
/codon_start=1
/product="Sec9p"
/protein_id="EWG91001.1"
/translation="MGLKKFFKIKPPEEATPEQNKDTLMELGISVKNPSKKRKEKFAA
YGKFANDKAEDKVYAPPGYEQYARPQDELEDLNASPLDANANEATAGSNRGSSGTQDL
GNGAESNSMQDPYAIENDDYRYDDDPYARFQANKSNGRGSVNAAPYGDYGGGYNGTSL
NSYNNDGPYSNQNTSNSWVNANGRNSLNHSNSTLNAGPSRQTRQPPVSTSTNSLSLDQ
RSPLANPMQEKRNPYADMNSYGGAYDSNTNRSSGTRQGSSKNANPYASMANDSYSNGN
LNRSANPYSSRSVRQPQSQQAPMTYTPSFIASDEAARNSEVDLNEEPRTGEFDFEEVY
ADKSAENRAALDEPDLNAVMTNEDSIDLNASEVDHRSRQQQQQQWFMDEQQQQQQQQH
FNATNNQYGDQRGYKTFEEIQKEEEARQQQEEDEAVDEIKQEIKFTKQSSVASTRNTL
KMAQDAERAGMNTLGMLGHQSEQLNNVEGNLDLMKVQNKVADEKVAELKKLNRSILAV
HVSNPFNSKRRRREREEQLKNRKIEEKLMREQTSQQLSQSTQRIEGAMNANNNISEVR
ERYQRKNVLEKAKRYQFENDEEDDEMELEIDRNLDQIQQVSNRLKKMALTTGKELDSQ
QKRLNNIEESTDDLDINLHMNTNRLAGIR"
gene 505771..506959
/gene="Nma2"
/locus_tag="P301_G11761"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 507386..508567
/locus_tag="P301_G11776"
mRNA 507386..508567
/locus_tag="P301_G11776"
/product="hypothetical protein"
CDS 507386..508567
/locus_tag="P301_G11776"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91002.1"
/translation="MSCSQNKTSVSLAWRECISIASVLIGAYASYRYYKLFKTRDIPR
PKEGIEELIGNTPLVKIRSLTKATGVNIYAKLELCNPAGSAKDRVALNIIKTAEELGE
LVRGEPGWVFEGTSGSTGISIAVVCNALGYRAHISLPDDTSLEKLALLESLGATVNKV
KPASIVDPNQYVNAAKKACNELKKSGNGIRAVFADQFENEANXXXHYQTTGPEIAHQT
KGNIDAFIAGCGTGGTITGVAKFLKERAKIPCHVVLADPQGSGFYNRVNYGVMYDYVE
KEGTRRRHQVDTIVEGIGLNRITHNFHMGEKFIDESIRVNDNQAIRMAKYLSVNDGLF
VGSSTAINAVAAIQVAKTLPHGSNIVIIACDSGSRHLSKFWKEAKEIDHDVSLEEVIN
I"
gene 508781..510643
/gene="Snu71"
/locus_tag="P301_G11781"
mRNA 508781..510643
/gene="Snu71"
/locus_tag="P301_G11781"
/product="Snu71p"
CDS 508781..510643
/gene="Snu71"
/locus_tag="P301_G11781"
/note="Similar to YGR013W 'Component of U1 snRNP required
for mRNA splicing via spliceosome; yeast specific, no
metazoan counterpart'"
/codon_start=1
/product="Snu71p"
/protein_id="EWG91003.1"
/translation="MRDIVFVSPQLYLSSQEGWKSDSAKSGFIPILKNDLQRFQDSLK
HIVDARNSLSETLLNSKDDGSIHNSDQNTGLNKDKEASIADNNSANKCATSSSRYQEL
KQFLPISLDQQIHTVSLQGVSSSFSRGQIESLLDHCLNLALTETQSNSALKVEAWSSF
SSFLDTQDIFIRFSKVDEDEAFVNTLNYCKALFAFIRKLHEDFKIELHLDLNTKEYVE
DRTGTIPSVKPEKASEFYSVFKNIEDQTDERNSKKEQLDDSSTQYKVDTNTLSDLPSD
ALDQLCKDIIEFRTKVVSIEKEKKMKSTYEESRRQRHQMQKVFDQIRKNHSGAKGSAN
TEEEDTNMEDEDEEDDTEDDLALEKRKEERDLEESNRRYEDMLHQLHSNTEPKIKSIR
ADIMSAENYEEHLEKNRSLYLKELLHLANDVHYDHHRSFKEQEERRDEEDRAKNGNAK
ELAPIQLSDGKAISAGKAAAITLPEGTVKSENYNADKNVSESSEHVKIKFDFKKAIDH
SVESSSEDEGYRESELPPTKPSERSAAEDRLPFTADELNIRLTNLKESRYVDELVREF
LGVYEDELVEYILENIRVNQSKQALLNELRETFDEDGETIADRLWSRKEFRLGT"
gene 511170..515089
/gene="Msb2"
/locus_tag="P301_G11786"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(515316..516302)
/locus_tag="P301_G11791"
mRNA complement(515316..516302)
/locus_tag="P301_G11791"
/product="hypothetical protein"
CDS complement(515316..516302)
/locus_tag="P301_G11791"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91004.1"
/translation="MSRLAHNKALPYKIIVDLSFHRTRLPSDVSSLIKFEQRPAIINI
HGLLGSHVMFHSLNKLLSRKLDADIFSVDVRNHGISPKAIPYDYTTLTNDLIYFIETH
IGLERPIYLLGFSMGGKIALLTTLYKNINIRKCISIDLPPYETPELDPMILQNYDLIM
RIIRRDVKILRGSPSWQKKVLELFKSLECNKRKCGGAVALYFANGFLSVKSNNVHQAQ
LHYEQQQHDPYINYSMPLSSMPNLLDEVKKWPDLSNQRDFFQKGTASRKVLFMKGLQS
NFINNDYSLLRYNFPCADVREFNTGHNLLLENPEDSFKCILNFFAEETLDFE"
gene 516487..517059
/locus_tag="P301_G11796"
mRNA 516487..517059
/locus_tag="P301_G11796"
/product="hypothetical protein"
CDS 516487..517059
/locus_tag="P301_G11796"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91005.1"
/translation="MSRLRRFNRKILSLSSDYTHDGESDQEDVSILPLDTEEQEELIQ
KFETNAHITNKLYINLLSILYLLYGGLLMILVRKSRGYIKLALLAGANSLICSCITLR
YDIVNDYLLFKKFKLRVSNFSINIINIILLVLMAWISFNHVVEDKKTVLCLQVPMFLF
WVAVLVKRWARNIEDEIADLRCLKYKYKNA"
gene 518012..518905
/locus_tag="P301_G11806"
mRNA 518012..518905
/locus_tag="P301_G11806"
/product="hypothetical protein"
CDS 518012..518905
/locus_tag="P301_G11806"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91006.1"
/translation="MSHQMAPWIPMFIQSCKNNTEPFVSFQFATVDELTNKPRCRTVV
FRDFLFHDKRTNVLTFNTDMRSSKITESFITPNSNNSSDSKRCETPFFEACFYFPETW
EQYRFSGQCFTISKQFKKIPAEIVTKYDIFSPRFSETNDDSTDEEIDTPINDDDDNDK
NNDADNNDINEDNKLIESIENDEHHEDEDDYYPQPQEWEAELLRQWSSLSRHTKSLYR
KPAPGQKLTSETSKQLDKLHRGVDGAKEDAGLENFGIVCLCVDSVDFLNLKEGRGGER
WIFQKTDGKDEDLWEEQEVCP"
gene 519454..520869
/gene="Uga1"
/locus_tag="P301_G11826"
mRNA 519454..520869
/gene="Uga1"
/locus_tag="P301_G11826"
/product="Uga1p"
CDS 519454..520869
/gene="Uga1"
/locus_tag="P301_G11826"
/note="Similar to YGR019W 'Gamma-aminobutyrate (GABA)
transaminase (4-aminobutyrate aminotransferase) involved
in the 4-aminobutyrate and glutamate degradation pathways;
required for normal oxidative stress tolerance and
nitrogen utilization'"
/codon_start=1
/product="Uga1p"
/protein_id="EWG91007.1"
/translation="MSICEQYYPEEPTKPTVKTESIPGPESQKQLKELGEVFDTRPAY
FLADYEKSLGNYITDVDGNTYLDLYAQISSIALGYNNPALIKAAQSPEMIRALVDRPA
LGNFPSKDLDKILKQILKSAPKGQDHVWSGLSGADANELAFKAAFIYYRAKQRGYDAD
FSEKENLSVMDNDAPGAPHLAVLSFKRAFHGRLFASGSTTCSKPIHKLDFPAFHWPHA
EYPSYQYPLDENSDANRKEDDRCLAIVEELIKTWSIPVAALIIEPIQSEGGDNHASKY
FLQKLRDITLKYNVVYIIDEVQTGVGATGKLWCHEYADIQPPVDLVTFSKKFQSAGYF
FHDPKFIPNKPYRQFNTWCGEPARMIIAGAIGQEISDKKLTEQCSRVGDYLFKKLEGL
QKKYPENFQNLRGKGRGTFIAWDLPTGEKRDLLLKKLKLNGCNVGGCAVHAVRLRPSL
TFEEKHADIFIEALAKSVNEL"
gene complement(521198..521554)
/gene="Vma7"
/locus_tag="P301_G11831"
mRNA complement(521198..521554)
/gene="Vma7"
/locus_tag="P301_G11831"
/product="Vma7p"
CDS complement(521198..521554)
/gene="Vma7"
/locus_tag="P301_G11831"
/note="Similar to YGR020C 'Subunit F of the eight-subunit
V1 peripheral membrane domain of vacuolar H+-ATPase
(V-ATPase), an electrogenic proton pump found throughout
the endomembrane system; required for the V1 domain to
assemble onto the vacuolar membrane'"
/codon_start=1
/product="Vma7p"
/protein_id="EWG91008.1"
/translation="MAEKRTLIAVIADEDTTTGLLLAGIGQITPETQEKNFFVYQEGK
TTKEEITDKFNHFTEERDDIAILLINQHIAENIRARVDSFTNAFPAILEIPSKDHPYD
PEKDSVLKRVRKLFGE"
gene 521856..522728
/locus_tag="P301_G11836"
mRNA 521856..522728
/locus_tag="P301_G11836"
/product="hypothetical protein"
CDS 521856..522728
/locus_tag="P301_G11836"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91009.1"
/translation="MLVRNRYLGELLKNSRSFSVLNSSVRSGHNKWSTIKHGKAKNDA
ERNKINNKFANQIAMSVKLGNGITDPSMNIRLATSIELANKNNVSKKVIENAIRKASG
SSASGKDSNASELCVYEGMGPGGVAIVVEALTDNKNRTIGLIRSAFNKANGSMTPTLF
FFDKKGYVTMVPPKMLDTEDKVLESVLEIQGIEDIAPVQEDAEDLECDTETETTGQTY
EAVMEPADTNKVAALLKERGFHIRDLGIGYNAKPDMEVFVQGDETLEKLQKLTTALED
IDEVTSLYTNASNA"
gene 523487..525205
/gene="Mtl1"
/locus_tag="P301_G11846"
mRNA 523487..525205
/gene="Mtl1"
/locus_tag="P301_G11846"
/product="Mtl1p"
CDS 523487..525205
/gene="Mtl1"
/locus_tag="P301_G11846"
/note="Similar to YGR023W 'Putative plasma membrane
sensor, involved in cell integrity signaling and stress
response during glucose starvation and oxidative stress;
has structural and functional similarity to Mid2p'"
/codon_start=1
/product="Mtl1p"
/protein_id="EWG91010.1"
/translation="MASCNPTRKKSSASSLSMWRTILMALTTLPLSVLSQELVPANST
TSSTAPSITSLSAVESFTSSTDATSSASLSTPSIASISFTSFPQSSSLLTLSSTLSSE
LSSSSMQVSSSSTSSSSSEVASSSSSSSISPSSSSSTIISSSSSLPTFTVASTSSTVA
SSTLSTSSSLVISTSSSTFTFSSESSSSLISSSISTSVSTSSVYVPSSSTSSPPSSSS
ELTSSSYSSSSSQSSSSSTLFSYSSSFSSSSSSSSSSSSSSSSSSSSSSSSSISLSSS
SSSSSSSSSYFTLSTSSSSSIYSSSSYPSFSSSSSSNPTSSITSTSASSSITPASEYS
NLAKTITSIIEGQTILSNYYTTITYSPTASASSGKDSHHSGLSKKNRNIIIGCVVGIG
APLILILLILIYMFCVQPKKTDFIDSDGKIVTAYRSNIFTKIWYFLLGKKIGETERFS
SDSPIGSNNIQNFGDIDPEDILNNDNPYTPKHANVEGYDDDDDDDANDENLSSNFHNR
GIDDQYSPTKSASYSMSNSNSQDYNDADEVMHDENIHRVYDDSEASIDENYYTKPNNG
LNITNY"
assembly_gap 525331..526234
/estimated_length=904
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<526235..526880)
/gene="Thg1"
/locus_tag="P301_G11851"
mRNA complement(<526235..526880)
/gene="Thg1"
/locus_tag="P301_G11851"
/product="Thg1p"
CDS complement(<526235..526880)
/gene="Thg1"
/locus_tag="P301_G11851"
/note="Similar to YGR024C 'tRNAHis guanylyltransferase,
adds a guanosine residue to the 5' end of tRNAHis after
transcription and RNase P cleavage; couples nuclear
division and migration to cell budding and cytokinesis;
essential enzyme conserved among eukaryotes'"
/codon_start=1
/product="Thg1p"
/protein_id="EWG91219.1"
/translation="MANSKFGYVRQFETHDVILPQCYIVVRIDGKKFHEFSKFYEFAK
PNDENALKLMNACAKNLVLKYKNDIILAFGESDEYSFILKSSTTLFNRRKDKLATLFG
SFFTSNYVALWAKFFPEKPLNIKHLPYFDSRCVAYPNLQTIKDYLSWRYVDTHINNLY
NTTFWQLIIKCGLTPQESEKKLCGTFSNEKQEILFSECGINYNNEPEMFKKGSLV"
gene 527269..528105
/locus_tag="P301_G11861"
mRNA 527269..528105
/locus_tag="P301_G11861"
/product="hypothetical protein"
CDS 527269..528105
/locus_tag="P301_G11861"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91011.1"
/translation="MAKTIKVIRKKDPKKKNLSDPLAKQKLVWKIGHVLTLVFGLLFS
ITYFYHVLIFFKYRSWKWLFLRVNKNYSFIQSKRWYMKLLSWSPQVMYRLSLIGVFMS
ESVTMQQNWVGLNPTWNDLLSSENFHTLLIACLWFFGGGKSFYKILPYMILSYLHLTK
MNYELNANKEEKIPLTPKDRKMLHLLAYSELLVILALTLDTILFKTGTSGFMLVIYVG
IYWLRLNFSPYAQVAVLELLVKFEKYVPKKYRDKWQVIKNFIYMKMKEHEKRTEEVAR
YA"
assembly_gap 528465..528740
/estimated_length=276
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 529865..529936
/locus_tag="P301_G11871"
tRNA 529865..529936
/locus_tag="P301_G11871"
/product="tRNA-Glu"
/note="TE(UUC)G3"
gene 530218..531306
/gene="Msp1"
/locus_tag="P301_G11876"
mRNA 530218..531306
/gene="Msp1"
/locus_tag="P301_G11876"
/product="Msp1p"
CDS 530218..531306
/gene="Msp1"
/locus_tag="P301_G11876"
/note="Similar to YGR028W 'Mitochondrial protein involved
in sorting of proteins in the mitochondria; putative
membrane-spanning ATPase'"
/codon_start=1
/product="Msp1p"
/protein_id="EWG91012.1"
/translation="MSRKFDLKTITDLSVLVGTGISLYYLVSRLLNDVESGQLSGKSR
ESKAKQSLQWEKLVKRSPALAEVTLDAYERTILSSIVTPDEINITFQDIGGLDPLISD
LHESVIYPLMMPEVYSNSPLLQAPSGVLLYGPPGCGKTMLAKALAKESGANFISIRMS
SIMDKWYGESNKIVDAMFSLANKLQPCIIFIDEIDSFLRERSSTDHEVTATLKAEFMT
LWDGLLNNGRVMIIGATNRINDIDDAFLRRLPKRFLVSLPGSDQRYKILSVLLKDTKL
DEDEFDLQLIADNTKGFSGSDLKELCREAALDAAKEYIKQKRQLIDSGTIDVNDTSSL
KIRPLKTKDFTKKLRMDATSTLSSQPLD"
gene 531568..532219
/gene="Erv1"
/locus_tag="P301_G11881"
mRNA join(531568..531653,531736..532219)
/gene="Erv1"
/locus_tag="P301_G11881"
/product="Erv1p"
CDS join(531568..531653,531736..532219)
/gene="Erv1"
/locus_tag="P301_G11881"
/note="Similar to YGR029W 'Flavin-linked sulfhydryl
oxidase of the mitochondrial intermembrane space (IMS),
oxidizes Mia40p as part of a disulfide relay system that
promotes IMS retention of imported proteins; ortholog of
human hepatopoietin (ALR)'"
/codon_start=1
/product="Erv1p"
/protein_id="EWG91013.1"
/translation="MKAIDKMTDNPPQEGLSGRKIIYDEDGKPCRSCNTLLDFQYVTG
KISNGLKNLSSNGKLAGTGALTGEASELMPGSRTYRKVDPPDVEQLGRSSWTLLHSVA
ASYPAQPTDQQKGEMKQFLNIFSHIYPCNWCAKDFEKYIRENAPQVESREELGRWMCE
AHNKVNKKLRKPKFDCNFWEKRWKDGWDE"
assembly_gap 532563..532753
/estimated_length=191
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 533112..533308
/locus_tag="P301_G11891"
ncRNA 533112..533308
/ncRNA_class="snoRNA"
/locus_tag="P301_G11891"
/product="SNR46"
gene complement(533423..533899)
/gene="Pop6"
/locus_tag="P301_G11896"
mRNA complement(533423..533899)
/gene="Pop6"
/locus_tag="P301_G11896"
/product="Pop6p"
CDS complement(533423..533899)
/gene="Pop6"
/locus_tag="P301_G11896"
/note="Similar to YGR030C Subunit of both RNase MRP, which
cleaves pre-rRNA, and nuclear RNase P, which cleaves tRNA
precursors to generate mature 5' ends"
/codon_start=1
/product="Pop6p"
/protein_id="EWG91014.1"
/translation="MINGVYYNEISRDLDISSSTQCLRFLKETVIPSLANNGNNSTSI
QYHGISKNDNIKKSVNKLDKQINIADRSLGLQQVVCIFSYGPHIQKMLSILEIFKKDY
IKSNKKIYQWNKLTSFDIKREGRNELQEERLKVPILVTLVSDSEIIDLNLHSFTKQ"
gene 534193..535221
/locus_tag="P301_G11901"
mRNA 534193..535221
/locus_tag="P301_G11901"
/product="hypothetical protein"
CDS 534193..535221
/locus_tag="P301_G11901"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91015.1"
/translation="MMILGKAGILAQYGTIYVRQNTIRNNLSSCIFKQSLCAFHSLAK
VLQQKQVPLDLSYDIIKRDAEKTGDEGKPRPPIIILHGLFGNKLNNRSIGRNLNKKLG
RDVYLLDLRNHGSSPHSSVHNYEVMSEDVKHFITKHELNTNGGPIIIGHSMGGKVAMM
LVLKNPQLCSMLVCIENAPVSLRPNAEFVEYIKALMEIVNDKGKTIRTLKQADEHLAE
RIGGNELVRRFLLTALKKVKMDNSSSVSSYTFEERIPLATLKDAIVKGEIAAWPLDPA
RERWTRPALFIRATQSHYVVDEYLPIIGAFFPRFETRDIDAGHWVNAEKPGECAESIV
DFVERHED"
gene 536015..541702
/gene="Gsc2"
/locus_tag="P301_G11906"
mRNA 536015..541702
/gene="Gsc2"
/locus_tag="P301_G11906"
/product="Gsc2p"
CDS 536015..541702
/gene="Gsc2"
/locus_tag="P301_G11906"
/note="Similar to YGR032W 'Catalytic subunit of
1,3-beta-glucan synthase, involved in formation of the
inner layer of the spore wall; activity positively
regulated by Rho1p and negatively by Smk1p; has similarity
to an alternate catalytic subunit, Fks1p (Gsc1p)'"
/codon_start=1
/product="Gsc2p"
/protein_id="EWG91016.1"
/translation="MSYNDPNLNGQYYSNGDGTGDGNYPTYQVTQDQSAYDEYGQPIY
TQNQLDDGYYDPNEQYVDGTQFPQGQDPSQDQGPYNNDASYYNQPPNMMNPSSQDGEN
FSDFSSYGPPSGTYPNDQYTPSQMSYPDQDGSSGASTPYGNGVVNGNGQYYDPNAIEM
ALPNDPYPAWTADPQSPLPIEQIEDIFIDLTNKFGFQRDSMRNMFDHFMTLLDSRSSR
MSPEQALLSLHADYIGGDTANYKKWYFAAQLDMDDEIGFRNMKLGKLSRKARKAKKKN
KKAMQEASPEDTEETLNQIEGDNSLEAADFRWKSKMNQLSPFEMVRQIALFLLCWGEA
NQVRFTPECLCFIYKCASDYLDSAQCQQRPDPLPEGDFLNRVITPLYCFIRSQVYEIV
DGRYVKSEKDHNKVIGYDDVNQLFWYPEGIAKIVMEDGTRLIDLPAEERYLKLGEIPW
DDVFFKTYKETRSWLHLVTNFNRIWIMHISVYWMYCAYNAPTFYTHNYQQLVDNQPLA
AYKWATAALGGTVASLIQVAATLCEWSFVPRKWAGAQHLSRRFWFLCVIMGINLGPVI
FVFAYDKDTVYSTAAHVVGAVMFFVAVATLVFFSVMPLGGLFTSYMKKSTRSYVASQT
FTASFAPLHGLDRWMSYLVWVTVFAAKYAESYFFLILSLRDPIRILSTTSMRCTGEYW
WGNKICKVQPKIVLGLMIATDFILFFLDTYLWYIVVNTVFSVGKSFYLGISILTPWRN
IFTRLPKRIYSKILATTDMEIKYKPKVLISQIWNAIIISMYREHLLAIDHVQKLLYHQ
VPSEIEGKRTLRAPTFFVSQDDNNFETEFFPRDSEAERRISFFAQSLSTPIPEPLPVD
NMPTFTVLTPHYAERILLSLREIIREDDQFSRVTLLEYLKQLHPVEWDCFVKDTKILA
EETAAYENNEDEPEKEDALKSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRT
ISGFMNYSRAIKLLYRVENPEIVQMFGGNADGLERELEKMARRKFKFLVSMQRLAKFK
PHELENAEFLLRAYPDLQIAYLDEEPPLSEGEEPRIYSALIDGHCEILENGRRRPKFR
VQLSGNPILGDGKSDNQNHALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEELGI
EQIHPYTPGLKYEDQSTNHPVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLA
QIGGKLHYGHPDFINATFMTTRGGVSKAQKGLHLNEDIYAGMNAVLRGGRIKHCEYYQ
CGKGRDLGFGTILNFTTKIGAGMGEQMLSREYYYLGTQLPIDRFLTFYYAHPGFHLNN
LFIQLSLQMFMLTLVNLHALAHESILCVYDRDKPITDVLYPIGCYNFHPAIDWVRRYT
LSIFIVFWIAFVPIVVQELIERGLWKATQRFFRHILSLSPMFEVFAGQIYSSALLSDI
AVGGARYISTGRGFATSRIPFSILYSRFAGSAIYMGSRSMLMLLFGTVAHWQAPLLWF
WASLSALIFAPFIFNPHQFAWEDFFLDYRDYIRWLSRGNNKYHRNSWIGYVRMSRSRV
TGFKRKLVGDESEKSAGDASRAHRTNLIMAEIIPCAIYAAGCFIAFTFINAQTGVKTT
DEDRVNSTLRIIICTLAPIVIDIGVLFFCMGLSCCSGPLLGMCCKKTGSVMAGIAHGI
AVVVHIVFFIVMWVLEGFSFVRMLIGVVTCIQCQRLIFHCMTVLLLTREFKNDHANTA
FWTGKWYSTGLGYMAWTQPTRELTAKVIELSEFAADFVLGHVILIFQLPVICIPKIDK
FHSIMLFWLKPSRQIRPPIYSLKQARLRKRMVRRYCSLYFLVLIIFAGCIVGPAVASA
HVSKDLGSGLKDTFHNLVQPRNVSNNDTGFQMSTYKSHYYTHTPSLKTWSTIK"
gene complement(541999..542718)
/gene="Tim21"
/locus_tag="P301_G11911"
mRNA complement(541999..542718)
/gene="Tim21"
/locus_tag="P301_G11911"
/product="Tim21p"
CDS complement(541999..542718)
/gene="Tim21"
/locus_tag="P301_G11911"
/note="Similar to YGR033C 'Nonessential subunit of the
Translocase of the Inner Mitochondrial membrane (TIM23
complex); interacts with the Translocase of the Outer
Mitochondrial membrane (TOM complex) and with respiratory
enzymes; may regulate TIM23 complex activity'"
/codon_start=1
/product="Tim21p"
/protein_id="EWG91017.1"
/translation="MSSSLPRSLLRLGHRKPLFPRYNTFVNSSVITHTSLLRARLYSN
GTGATSGKKDDKTRNKPKPLWPQVKSASTFTFSGILVIGAVGISAIVIYLILSELFSP
SGDTQLFNRAVSMVEKNKDIRSLLQCDDGITGKERLKAYGELITNDKWTRNRPIVSTK
KLDKEGRTHHYMRFHVESKKKIALVHLEAKESKQNYQPDFINMYVDVPGEKRYYLIKP
KLHPVSNSKGFLGIRWGPRKD"
assembly_gap 542990..543045
/estimated_length=56
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 543560..544518
/gene="Rpl26b"
/locus_tag="P301_G11916"
mRNA join(543560..543578,544154..544518)
/gene="Rpl26b"
/locus_tag="P301_G11916"
/product="Rpl26bp"
CDS join(543560..543578,544154..544518)
/gene="Rpl26b"
/locus_tag="P301_G11916"
/note="Similar to YGR034W 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl26Ap and
has similarity to E. coli L24 and rat L26 ribosomal
proteins; binds to 5.8S rRNA'"
/codon_start=1
/product="Rpl26bp"
/protein_id="EWG91018.1"
/translation="MAKQSLDVSSDRRKARKAYFTAPSSERRVLLSAPLSKELRAQYG
IKALPIRRDDEVLVVRGSKKGQEGKISSVYRLKFAVQVDKVTKEKVNGASVPINLHPS
KLVITKLHLDKDRKALIQRKGGKLE"
assembly_gap 543843..544002
/estimated_length=160
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 544838..544860
/estimated_length=23
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(544918..545268)
/locus_tag="P301_G11921"
mRNA complement(544918..545268)
/locus_tag="P301_G11921"
/product="hypothetical protein"
CDS complement(544918..545268)
/locus_tag="P301_G11921"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91019.1"
/translation="MLLTPAKTTRTEDSANSTDDSSKSSNSFMRAIVSSLMVKPITSL
TNTVTCRQSSHHNSSPSKITRYDLIKAAAENDLKRSKSQGREKSRRNSNRRNNEEIFV
ANTASEIQRTKSSI"
gene 545411..545767
/locus_tag="P301_G11926"
mRNA 545411..545767
/locus_tag="P301_G11926"
/product="hypothetical protein"
CDS 545411..545767
/locus_tag="P301_G11926"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91020.1"
/translation="MLLYLYILYIALKTVSHLFFCNPCFRNLSVGDMLNPRLSLSFFT
NHLLCPEAPLIIRGKGSYSAVGTSSREKSEFTRGALVKPEGRFFPSVEQSAPRRQRVL
SPQVIELTFILRSAWF"
gene complement(545994..546713)
/gene="Cax4"
/locus_tag="P301_G11931"
mRNA complement(545994..546713)
/gene="Cax4"
/locus_tag="P301_G11931"
/product="Cax4p"
CDS complement(545994..546713)
/gene="Cax4"
/locus_tag="P301_G11931"
/note="Similar to YGR036C Dolichyl pyrophosphate (Dol-P-P)
phosphatase with a luminally oriented active site in the
ER, cleaves the anhydride linkage in Dol-P-P, required for
Dol-P-P-linked oligosaccharide intermediate synthesis and
protein N-glycosylation"
/codon_start=1
/product="Cax4p"
/protein_id="EWG91021.1"
/translation="MNSTAAAINPNPNVIPFDDTYILYDSHDFLSFLSAYFSLMPILV
LAFYLSWFIITRELEACIVAFGQLMNEIFNNVIKNIIKQPRPVSFGASFQNDTIRSGY
GMPSAHSQFMGFCFTYNSLKIYTSWKNLNFLEKCIFSGALALLSFCVCFSRVYLHYHN
LDQVIVGFSVGALTGSLYFFIVGIIRELGLINWFLKLRIVRLFYMTDSYNLAPLTLKE
NYEAYWKRINQRSFNDKSKRD"
gene complement(547576..547839)
/gene="Acb1"
/locus_tag="P301_G11941"
mRNA complement(547576..547839)
/gene="Acb1"
/locus_tag="P301_G11941"
/product="Acb1p"
CDS complement(547576..547839)
/gene="Acb1"
/locus_tag="P301_G11941"
/note="Similar to YGR037C 'Acyl-CoA-binding protein,
transports newly synthesized acyl-CoA esters from fatty
acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming
processes; subject to starvation-induced, Grh1p-mediated
unconventional secretion'"
/codon_start=1
/product="Acb1p"
/protein_id="EWG91022.1"
/translation="MVSQLFEEKAKAVNELPTKPSTDELLELYALYKQATVGDNDKEK
PGIFNMKDRYKWEAWENLKGKSQEDAEKEYIALVDQLIAKYSS"
gene 548528..549196
/gene="Orm1"
/locus_tag="P301_G11946"
mRNA 548528..549196
/gene="Orm1"
/locus_tag="P301_G11946"
/product="Orm1p"
CDS 548528..549196
/gene="Orm1"
/locus_tag="P301_G11946"
/note="Similar to YGR038W 'Evolutionarily conserved
protein, similar to Orm2p, required for resistance to
agents that induce unfolded protein response; Orm1p and
Orm2p together control membrane biogenesis by coordinating
lipid homeostasis with protein quality control'"
/codon_start=1
/product="Orm1p"
/protein_id="EWG91023.1"
/translation="MTELDYQGTAEAASTSYSRNQTDLKPFPSAGSASSSIKTTEPVK
DHRRRRSSSIISHVEPETFEDENDQQLLPNMNATWVDQRGAWIIHVVIIILLKLFYNL
FPGVTTEWSWTLTNMTYVIGSYVMFHLIKGTPFDFNGGAYDNLTMWEQIDDETLYTPS
RKFLISVPIALFLVSTHYAHYDLKLFSWNCFLTTFGAVVPKLPVTHRLRISIPGITGR
AQIS"
gene complement(549509..549590)
/locus_tag="P301_G11951"
tRNA complement(549509..549590)
/locus_tag="P301_G11951"
/product="tRNA-Ser"
/note="TS(AGA)G"
assembly_gap 549612..551298
/estimated_length=1687
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 551970..553076
/gene="Kss1"
/locus_tag="P301_G11961"
mRNA 551970..553076
/gene="Kss1"
/locus_tag="P301_G11961"
/product="Kss1p"
CDS 551970..553076
/gene="Kss1"
/locus_tag="P301_G11961"
/note="Similar to YGR040W 'Mitogen-activated protein
kinase (MAPK) involved in signal transduction pathways
that control filamentous growth and pheromone response;
the KSS1 gene is nonfunctional in S288C strains and
functional in W303 strains'"
/codon_start=1
/product="Kss1p"
/protein_id="EWG91024.1"
/translation="MARTITFDIPSQYKLVDLIGEGAYGTVCSAIHKPSGIKVAIKKI
QPFSKKLFVTRTIREIKLLRYFHEHENIISILDKVRPVSIDKLNAVYLVEELMETDLQ
KVINNQNSGFSTLSDDHVQYFTYQILRALKSIHSAQVIHRDIKPSNLLLNSNCDLKVC
DFGLARCLASSSDSRETLVGFMTEYVATRWYRAPEIMLTFQEYTTAMDIWSCGCILAE
MVSGKPLFPGRDYHHQLWLILEVLGTPSFEDFNQIKSKRAKEYIANLPMRPPLPWETV
WSKTDLNPDMIDLLDKMLQFNPDKRISAAEALRHPYLAMYHDPSDEPEYPPLNLDDEF
WKLDNKIMRPEEEEEVPIEMLKDMLYDELMKTME"
gene 554058..555701
/gene="Bud9"
/locus_tag="P301_G11971"
mRNA 554058..555701
/gene="Bud9"
/locus_tag="P301_G11971"
/product="Bud9p"
CDS 554058..555701
/gene="Bud9"
/locus_tag="P301_G11971"
/note="Similar to YGR041W 'Protein involved in bud-site
selection; mutant has increased aneuploidy tolerance;
diploid mutants display a unipolar budding pattern instead
of the wild-type bipolar pattern, and bud at the distal
pole'"
/codon_start=1
/product="Bud9p"
/protein_id="EWG91025.1"
/translation="MTKITRDVSITTENSKSTSGSATASSASLPENDHPIFHQPRARI
RSGSLFIEGSDSFPSSEVKSYNVYIDDSKYSEILKGDTNSSSTDGKQVFEDARDDNFH
QESHRDLEDSILDLVRRDPEVAAFPLPPPNSNERNRNSSNGSSAETNLNGHSSSGTIS
TSVLLNMGSAEKHAGTTRGDHMESSSMKSFEKLGTRPSSLFYPPPEGTAPYQGPRATV
SGNKSTRQTQGTYSFPSMRYGVDLVSPVEGAVDVAKSRVPNSTLNGTFPDKAFIPHEF
QIPKKAWNRIPANKSTSLKTPRNHSLLIDILKPFEAADLANDQRSSSAVLKNTVHSNG
QYNPTNETSGTRMQDQRQKNTNEIDLEKIPNPQVPLGIAMDTMRSPNQLHEKEYESNI
EAGLASGVGKGDNSIKQHQYKKIPQEIDRDQQLSFQMETMPIQRIDSSSIRSFDSRIY
GFSEIYSIPRVITTLCICLFVPPLFFFFSINGNNGVSNYRLMRMIMNYEHRIGLLKGF
EWDIDVQWFRTLCFVLGCIEMLAIFASIGIGFGVGITRE"
gene 556047..556861
/locus_tag="P301_G11976"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(557005..558006)
/gene="Nqm1"
/locus_tag="P301_G11981"
mRNA complement(557005..558006)
/gene="Nqm1"
/locus_tag="P301_G11981"
/product="Nqm1p"
CDS complement(557005..558006)
/gene="Nqm1"
/locus_tag="P301_G11981"
/note="Similar to YGR043C 'Transaldolase of unknown
function; transcription is repressed by Mot1p and induced
by alpha-factor and during diauxic shift'"
/codon_start=1
/product="Nqm1p"
/protein_id="EWG91026.1"
/translation="MSEPSEKKQKVATSSLEQLKKAGTHVVADSGDFEAISKYEPQDS
TTNPSLILAASKLEKYARFIDAAVEYGRKHGKTDHEKIENAMDKILVEFGTQILKVVP
GRVSTEVDARLSFDKKATVKKALHIIKLYKDAGVPKERVLIKIASTWEGIQAARELEV
KHGIHCNMTLLFSFTQAVACAEANVTLISPFVGRIMDFYKALSGKDYTAETDPGVLSV
KKIYSYYKRHGYATEVMAASFRNLDELKALAGIDNMTLPLNLLEQLYESTDPIENKLN
SESAKEEGVEKVSFINDEPHFRYVLNEDQMATEKLSDGIRKFSADIEALYKLVEEKM"
gene complement(559562..560464)
/gene="Rme1"
/locus_tag="P301_G11991"
mRNA complement(559562..560464)
/gene="Rme1"
/locus_tag="P301_G11991"
/product="Rme1p"
CDS complement(559562..560464)
/gene="Rme1"
/locus_tag="P301_G11991"
/note="Similar to YGR044C 'Zinc finger protein involved in
control of meiosis; prevents meiosis by repressing IME1
expression and promotes mitosis by activating CLN2
expression; directly repressed by a1-alpha2 regulator;
mediates cell type control of sporulation'"
/codon_start=1
/product="Rme1p"
/protein_id="EWG91027.1"
/translation="MSPCYGQNSAIAKGSWNREVLQEVQPIYHWHDFGQNMKEYSASP
LEGDSSLPSSLPSSTEDCLLLSLENTITVIAGNQRQAYDSTSSTEEGTAPQLRPDEIA
DSTHCITSLVDPEFRDLINYGRQKGANPVFIESNTTEQSHSQCILGYPQKSHVAQLYH
DPKVLSTISEGQTKRGSYHCSHCSEKFATLVEFAAHLDEFNLERPCKCPIEQCPWKIL
GFQQATGLRRHCASQHIGELDIEMEKSLNLKVEKYPGLNCPFPICQKTFRRKDAYKRH
VAMVHNNADSRFNKRLKKILNNTK"
gene 561467..562624
/gene="Tam41"
/locus_tag="P301_G12001"
mRNA 561467..562624
/gene="Tam41"
/locus_tag="P301_G12001"
/product="Tam41p"
CDS 561467..562624
/gene="Tam41"
/locus_tag="P301_G12001"
/note="Similar to YGR046W 'Mitochondrial protein involved
in protein import into the mitochondrial matrix; maintains
the functional integrity of the TIM23 protein translocator
complex; viability of null mutant is strain-dependent;
mRNA is targeted to the bud'"
/codon_start=1
/product="Tam41p"
/protein_id="EWG91028.1"
/translation="MLRVSENSLRFLLKCHSTNVSMFNRLLSTQIKEGRSSIDDAGII
PDGTINERPNHYIEGITKGSDLDLLEKGIRKTDEMTSNFTNYMYKFHRLPPNYGSNQL
ITIDKELQKELDGVMSSFKAPCRFVFGYGSGVFEQAGYSKSHSKPQIDIILGVTYPSH
FHSINMRQNPQHYSSLKYFGSEFVSKFQQIGAGVYFNPFANINGHDVKYGVVSMETLL
KDIATWNTFYLAGRLQKPVKILKNDLRVQYWNQLNLKAAATLAKHYTLEKNNNKFDEF
QFYKEITALSYAGDIRYKLGGENPDKVNNIVTKNFERFQEYYKPIYKEVVLNDSFYLP
KGFTLKNTQRLLLSRISKSSALQTIKGVFTAGITKSIKYAWAKKLKSMRRS"
gene complement(562964..566041)
/gene="Tfc4"
/locus_tag="P301_G12006"
mRNA complement(562964..566041)
/gene="Tfc4"
/locus_tag="P301_G12006"
/product="Tfc4p"
CDS complement(562964..566041)
/gene="Tfc4"
/locus_tag="P301_G12006"
/note="Similar to YGR047C 'One of six subunits of the RNA
polymerase III transcription initiation factor complex
(TFIIIC); part of the TauA domain of TFIIIC that binds
BoxA DNA promoter sites of tRNA and similar genes; has TPR
motifs; human homolog is TFIIIC-102'"
/codon_start=1
/product="Tfc4p"
/protein_id="EWG91029.1"
/translation="MAAGKLKKEQQNQSAERESADTGKVNDEDEEHLYGNIDDYKHLI
QDEEYDDEDVPHDLQLSEDEYNSERDSSLLAEFSDYGEISEDDEEDFMNAIREASNFK
VKKKKKNDKGKSYGRQRKERVLDPEVAQLLSQANEAFVRNDLQVAERLFNEVIKKDAR
NFAAYETLGDIYQLQGRLNDCCNSWFLAAHLNASDWEFWKIVAILSADLDHVRQAIYC
FSRVISLNPMEWESIYRRSMLYKKTGQLARALDGFQRLYMYNPYDANILRELAILYVD
YDRIEDSIELYMKVFNANVERREAILAALENALDSSDEESAAEGEDADEKEPLEQDED
RQMFPDINWKKIDAKYKCIPFDWSSLNILAELFLKLAVSEVDGIKTIKKCARWIQRRE
SQTFWDHVPDDSEFDNRRFKNSTFDSLLAAEKEKSYNIPIDIRVRLGLLRLNTDNLVE
ALNHFQCLYDETFSDVADLYFEAATALTRAEKYKEAIDFFTPLLSLEEWRTTDVFKPL
ARCYKEIESYETAKEFYELAIKSEPDDLDIRVSLAEVYYRLNDPETFKHMLVDVVEMR
KHQVDETLHRISNEKSSNDTSDISSKPLLEDSKFRTFRKKKRTPYDAERERIERERRI
TAKVVDKYEKMKKFELNSGLNEAKQASIWINTVSELVDIFSSVKNFFMKSRSRKFVGI
LRRTKKFNTELDFQIERLSKLAEGDSVFEGPLMEERVTLTSATELRGLSYEQWFELFM
ELSLVIAKYQSVEDGLSVVETAQEVNVFFQDPERVKMMKFVKLAIVLQMDDEEELAEN
LRGLLNQFQFNRKVLQVFMYSLCRGPSSLNILSSTIQQKFFLRQLKAFDSCRYNTEVN
GQASITNKEVYNPNKKSSPYLYYIYAVLLYSSRGFLSALQYLTRLEEDIPDDPMVNLL
MGLSHIHRAMQRLTAQRHFQIFHGLRYLYRYHKIRKSLYTDLEKQEADYNLGRAFHLI
GLVSIAIEYYNRVLENYDDGKLKKHAAYNSIIIYQQSGNVELADHLMEKYLSI"
gene 566390..567475
/gene="Ufd1"
/locus_tag="P301_G12011"
mRNA 566390..567475
/gene="Ufd1"
/locus_tag="P301_G12011"
/product="Ufd1p"
CDS 566390..567475
/gene="Ufd1"
/locus_tag="P301_G12011"
/note="Similar to YGR048W 'Protein that interacts with
Cdc48p and Npl4p, involved in recognition of
polyubiquitinated proteins and their presentation to the
26S proteasome for degradation; involved in transporting
proteins from the ER to the cytosol'"
/codon_start=1
/product="Ufd1p"
/protein_id="EWG91030.1"
/translation="MFSGFSSFGGGNGFVNMPQTFEEFFRCYPIAMMNDRIRKDDANF
GGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVTHGGVLEFIAEEGRVYLPQWMME
TLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLTVDDVI
EISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEK
KNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVGQGQNISGKAPKAEPKQ
DIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSKSSEQNFQGQGISLRK
SNKRKTKSDHDSSKSKAPKSPEVIEID"
gene 567877..>568435
/gene="Scm4"
/locus_tag="P301_G12026"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 568436..568526
/estimated_length=91
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 570155..571264
/gene="Fmp48"
/locus_tag="P301_G12051"
mRNA 570155..571264
/gene="Fmp48"
/locus_tag="P301_G12051"
/product="Fmp48p"
CDS 570155..571264
/gene="Fmp48"
/locus_tag="P301_G12051"
/note="Similar to YGR052W 'Putative protein of unknown
function; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies;
induced by treatment with 8-methoxypsoralen and UVA
irradiation'"
/codon_start=1
/product="Fmp48p"
/protein_id="EWG91031.1"
/translation="MYTKLRSIQSGTFSTVYKAWSTTHNRYVALKITPKYKTSEANMK
NEYDVMKILSSCNPHPNICSMLDFYTDDSYYIMVLEYCECGDLYDFLDIAKSQGSPSS
PSLIQIDMQKIIKQLCSAISFAHSLGIAHRDIKPENILLTINGDIKLADWGHAIQSPK
SNDFQIGTDNYRAPETFSGRVSNSCFKKNFDRSSAPLYNTYQADYWSLGATIFYLMFG
DCLFRVSKSKKVQHLKNFDEFEKDPFAFIYRKYVVPRLSCGYNDEEDLHVSLQHMRQY
IWQDLPDIYDVFHLCKIMVDTLLKVSNVIERSMENFINEVDSAWNKDSSMDSCFSYQN
KIDLFWEQWSVNTETVPAKFQLKNFEKPCLIQDGK"
gene complement(571543..572394)
/locus_tag="P301_G12056"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 573249..575177
/locus_tag="P301_G12061"
mRNA 573249..575177
/locus_tag="P301_G12061"
/product="hypothetical protein"
CDS 573249..575177
/locus_tag="P301_G12061"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91032.1"
/translation="MSSQFFLKTSQDIELFQSYPTFEQSNTNSKDFPVISSVLSPCGR
FLALSTKENVKVFTGPCLDNVLLTMKLSDVYDLHFSPAGNYLSTWERASIQDPNHKNV
KVWYLNKPFKKDCVSEDIVPAYEYQAKSQSGWFLQFSKLDNYGLRLFKHDLKIVKLSS
ANADNFDFQSPFAVLSDDETSQHFTTYLISPAEHPTICTFTPEKGGKPAQLIIWALSE
GKITKKIASKTFFKADSCQLKWNPLGNAILCLAITDFDSSNKSYYGENTLYLLSFQGV
NGTLGGNSVRVSLTTGPVHDFTWSPTSRQFGVIAGYMPATISFFDLRGNVVHSLPQQA
KNTMLFSPSGHYILIAGFGNLQGSVEILDRLDKFKCVSKFDATNTSVCKWSPGGEFIM
TATTSPRLRVDNGVKIWHVSGSLVFVKEFKELLKVDWRSPCNYKTLENKDEAFFENHI
INNWEPLPDSTTSSLDPKISNKSELQIHSSVQEYISQHPSREASSNGNGSKAKAGGAY
KPPHARRTGGGRIVPGVPPGAAKKTIPGLVPGMSANKDANTKNRRRRANKKSSETSPD
STPAPSAPASTNAPTNNKETSPEEKKIRSLLKKLRAIETLKERQAVGDKLEDTQVLKI
QTEEKVLKDLEKLGWKDE"
gene 575971..577696
/gene="Mup1"
/locus_tag="P301_G12066"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 578216..581002
/gene="Rsc1"
/locus_tag="P301_G12071"
mRNA 578216..581002
/gene="Rsc1"
/locus_tag="P301_G12071"
/product="Rsc1p"
CDS 578216..581002
/gene="Rsc1"
/locus_tag="P301_G12071"
/note="Similar to YGR056W 'Component of the RSC chromatin
remodeling complex; required for expression of mid-late
sporulation-specific genes; contains two essential
bromodomains, a bromo-adjacent homology (BAH) domain, and
an AT hook'"
/codon_start=1
/product="Rsc1p"
/protein_id="EWG91033.1"
/translation="MVEQDNGFLQKLLKTQYDAVFHLKDENGIEIYPIFNVLPPKKEY
PDYYIIIRNPISLNTLKKRLPHYTSPQDFVNDFAQIPWNAMTYNAKDSVIYKYAILLE
SFIKGKIVHNIRKHYPEVTYPSLGRIPEIFAESMQPSDLSSNPINTQENDEKAGLNPE
MKMAFAKLDSSITERKPTNQDYRMQQKNSPAFPTHSASITPQPLASPTPVVNYANITS
AHPKTHVRRGRPPVIDLPYVLRIKNILKMMRREVDQNNKTLTLCFEKLPDRNEEPTYY
SVITDPICLMDIRKKVKSRKYRNFHTFEEDFQLMLTNFKLYYSQDQSNIIRAQLLEKN
FNRLVRIELSKPDEDYLPEGELRYPLDDVEINDEKYQIGDWVLLHNPNDINKPIVGQI
FRLWSTTDGNKWLNACWYFRPEQTVHRVDRLFYKNEVMKTGQYRDHPIQDIKGKCYVI
HFTRFQRGDPSTKVNGPQFVCEFRYNESDKVFNKIRTWKACLPEELRDQDEPTIPVNG
RKFFKYPSPIADLLPANATLNDKVPEPTEGAPTAPPLVGAVYLGPKLERDDLGEYSTS
DDCPRYIIRPNDPPEEGKIDYETGTIITDTLTTSSMPRVNSSSTIRLPTLKQTKSIPS
SNFRSSSNTPLLHQNFNQTSNFLKLENMNNSSHNLLSHPSVPKFQSPSLLEQSSRSKY
HSAKKQTQLSSTAPKKPASKSFTLSSMINTLTAHTSKYNFNHIVIEAPGAFVVPVPME
KNIRTIQSTERFSRSNLKNAQNLGNTAINDINTANEQIIWFKGPGVKITERVIDSGND
LVRVPLNRWFCKNKRRKLDYEEIEEDVMEPPNDFSEDMIANIFNPPPSLNLDMDLNLS
PSSNNSSNFMDLSTIASGDNDGKECDTAEESEDENEDTEDEHEIEDIPTTSAFGLNSS
AEYLAFRLREFNKL"
gene complement(581328..581876)
/gene="Lst7"
/locus_tag="P301_G12076"
mRNA complement(581328..581876)
/gene="Lst7"
/locus_tag="P301_G12076"
/product="Lst7p"
CDS complement(581328..581876)
/gene="Lst7"
/locus_tag="P301_G12076"
/note="Similar to YGR057C 'Protein possibly involved in a
post-Golgi secretory pathway; required for the transport
of nitrogen-regulated amino acid permease Gap1p from the
Golgi to the cell surface'"
/codon_start=1
/product="Lst7p"
/protein_id="EWG91034.1"
/translation="MRCFIEDVPFITTQYSSIRYQLLNSIIKRAFSEETMIYDNMPFI
FFDDLRGLNLVIGFKLYDENARGNERRYCFILTVDSRSHDDSMKMLSEHWNFIIGGFD
KMIAYIKNIHKSEFLGKNKTVENNLETLNNNAFIGSYLRANKSKFGRNLVSLTDDKFL
FVRIHKWNSFLLHTVMNENKLP"
assembly_gap 582056..582122
/estimated_length=67
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 582691..583698
/gene="Pef1"
/locus_tag="P301_G12081"
mRNA 582691..583698
/gene="Pef1"
/locus_tag="P301_G12081"
/product="Pef1p"
CDS 582691..583698
/gene="Pef1"
/locus_tag="P301_G12081"
/note="Similar to YGR058W 'Penta-EF-hand protein required
for polar bud growth and cell wall abscission; binds
calcium and zinc with different affinity; localizes to bud
site in G1, bud neck in G2'"
/codon_start=1
/product="Pef1p"
/protein_id="EWG91035.1"
/translation="MCAKKLKYAAGDDFVRYATPKEAMEETRREFEKEKQRQQQIKVT
QAQTPNTRVHSAPIPLQTQYNKNRAENGHHSYGSPQSYSPRHTKTPVDPRYNVIAQKP
AGRPIPPAPTHYNNLNTSAQRIASSPPPLIHNQAVPAQLLKKVAPASFDSREDVRDMQ
VATQLFHNHDVKGKNRLTAEELQNLLQNDDNSHFCISSVDALINLFGASRFGTVNQAE
FIALYKRVKSWRKVYVDNDINGSLTISVSEFHNSLQELGYLIPFEVSEKTFDQYAEFI
NRNGTGKELKFDKFVEALVWLMRLTKLFRKFDTNQEGIATIQYKDFIDATLYLGRFLP
H"
gene 584115..585653
/gene="Spr3"
/locus_tag="P301_G12086"
mRNA 584115..585653
/gene="Spr3"
/locus_tag="P301_G12086"
/product="Spr3p"
CDS 584115..585653
/gene="Spr3"
/locus_tag="P301_G12086"
/note="Similar to YGR059W 'Sporulation-specific homolog of
the yeast CDC3/10/11/12 family of bud neck microfilament
genes; septin protein involved in sporulation; regulated
by ABFI'"
/codon_start=1
/product="Spr3p"
/protein_id="EWG91036.1"
/translation="MKSKGSRLSTDCPVEFPKIVSGFAEEVKIRRQSSQGQYAVDSHP
PKSPELKHRRQRSSSFVNGKCRNRDLPLLDNKKAQEINTNSHGQDIGIKNLPRQRELL
NAKNGIDFTLMVAGQSGLGKTTFINSLFSTSLIDDDIKENKPIIRYKSIVEGDGTHLN
FNVIDTPGFGNNMDNAFTWRTMVNYIDEEIRSYIFQEEQPDRTKMVDNRVHCCLYFLR
PSNKGIDTLDVVTMKKLAKRVNLIPVIAKSDLLTKEELKNFKTQVREIIRVQDIPVCF
FFGDEVLNATQDIFQKYPFSIIASNEYIFNEKGEKVKGRQYKWGAVDIENEKYCDFKI
LQKTIFDWNLIDLVESTEDYYEKCRSEMLRTRLLKARDCLTTKSVDITEEQRKFLEEE
MNFDEIEENKLKNYKCYEIINKTVMDKVATKWDPEFITRQLEAKKKFNELSNREISKF
RDWKKSLFMEQENFNQEIEQLNHKLENLQLECQDLEYKLLIGKSSNSHSTDSATLVNV
HIKR"
gene 586133..586245
/locus_tag="P301_G12091"
ncRNA 586133..586245
/ncRNA_class="snoRNA"
/locus_tag="P301_G12091"
/product="SNR48"
gene 587111..588040
/gene="Erg25"
/locus_tag="P301_G12096"
mRNA 587111..588040
/gene="Erg25"
/locus_tag="P301_G12096"
/product="Erg25p"
CDS 587111..588040
/gene="Erg25"
/locus_tag="P301_G12096"
/note="Similar to YGR060W 'C-4 methyl sterol oxidase,
catalyzes the first of three steps required to remove two
C-4 methyl groups from an intermediate in ergosterol
biosynthesis; mutants accumulate the sterol intermediate
4,4-dimethylzymosterol'"
/codon_start=1
/product="Erg25p"
/protein_id="EWG91037.1"
/translation="MSAVFNNATLSGLVQASTYSQTLQNVAHYQPQLNFMEKYWAAWY
SYMNNDVLATGLMFFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTKIPSAKEQLYCL
KSVLLSHFLVEAIPIWTFHPMCEKLGITVEVPFPSLKTMALEIGLFFVLEDTWHYWAH
RLFHYGVFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPILYVMYTGKLHL
FTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDLHHHYFIGNYASSFRWW
DYCLDTESGPEAKASREERMKKRAENNAQKKTN"
assembly_gap 588105..588530
/estimated_length=426
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<588531..592514)
/gene="Ade6"
/locus_tag="P301_G12101"
mRNA complement(<588531..592514)
/gene="Ade6"
/locus_tag="P301_G12101"
/product="Ade6p"
CDS complement(<588531..592514)
/gene="Ade6"
/locus_tag="P301_G12101"
/note="Similar to YGR061C
Formylglycinamidine-ribonucleotide (FGAM)-synthetase,
catalyzes a step in the 'de novo' purine nucleotide
biosynthetic pathway"
/codon_start=1
/product="Ade6p"
/protein_id="EWG91220.1"
/translation="MTDYILPGPKALSQFRVDNLIKDINSYTNSTSVINELRSCYIHY
VNGIAQNLSEQDTKLLEVLLTYDSPLDIANDPLARQLNDAVANNLPSSALGEDTYLIR
VVPRSGTISPWSSKATNIAHVCGLQDKVQRIERGLALLIKTVPGFPLLENLNDISLKC
VYDRMTQQLYLTEPPNTMSIFTHEEPKPLVHVPLTPKDTKQSPKDILSKANTELGLAL
DSGEMEYLIHAFVETMKRDPTDVELFMFAQVNSEHCRHKIFNADWTIDGIKQQFTLFQ
MIRNTHKLNPEYTISAYSDNAAVLDSENDAFFFAPNSTTKEWTSTKERIPLLIKVETH
NHPTAVSPFPGAATGSGGEIRDEGATGRGSKTKCGLSGFSVSDLLIPGNEQPWELNIG
KPYHIASALDIMIEAPLGSAAFNNEFGRPCINGYFRTLTTKVLNHQGKEEIRGFHKPI
MIAGGFGTVRPQFALKNTPITPGSCLIVLGGQSMLIGLGGGAASSVASGEGSADLDFA
SVQRGNPEMERRCQQVIDACVALGNNNPIQSIHDVGAGGLSNALPELVHDNDLGAKFD
IRKVLSLEPGMSPMEIWCNESQERYVLGVSPQDLSIFEEICKRERAPFAVVGHATAEQ
KLIVEDPLLKTTPIDLEMPILFGKPPKMSRETITEALNLPEANLSEIPSLQDAIQRVL
NLPSVGSKSFLITIGDRSVTGLIDRDQFVGPWQVPVADVGVTGTSLGETIISTGEAMA
MGEKPVNALISASASAKLSVAESLLNIFAADVKSLNHIKLSANWMSPASHQGEGSKLY
EAVQALGLDLCPALGVAIPVGKDSMSMKMKWNDKEVTAPLSLNITAFAPVFNTSKTWT
PLLNRNTDDSVLVLVDLSAKQETKSLGASALLQVYNQVGNKSPTVYDNAILKGFLESL
IQLHQQKEDIVLAYHDRSDGGLLITLLEMAFASRCGLEINIDGGDLESQLTNLFNEEL
GAVFQISAKNLSKFEKILNENGVAKEYISIVGKPSFQSQEIKIINSTTNDVIYANSRS
ELEQTWSKTSYEMQKLRDNPKTAEEEFASITDDRDPGLQYALTYNPADDMKIGLELSS
QRPKVAILREQGVNGQMEMAWCFQQAGFNSVDVTMTDLLEGRFHLDDFIGLAACGGFS
YGDVLGAGAGWAKSVLYHEGVRSQFSKFFNERQDTFAFGACNGCQFLSRLKDIIPGCE
NWPSFERNVSEQYEARVCMVQISQEKDNSSEESVFLNGMAGSKLPIAVAHGEGKATFS
KSAEQLEKFEKDGLCCIRYVDNYGNVTERFPFNPNGSTNGIAGIKSPNGRVLAMMPHP
ERVCRLEA"
gene complement(592875..593825)
/gene="Cox18"
/locus_tag="P301_G12106"
mRNA complement(592875..593825)
/gene="Cox18"
/locus_tag="P301_G12106"
/product="Cox18p"
CDS complement(592875..593825)
/gene="Cox18"
/locus_tag="P301_G12106"
/note="Similar to YGR062C 'Mitochondrial integral inner
membrane protein required for membrane insertion of
C-terminus of Cox2p; interacts genetically and physically
with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N.
crassa Oxa2p, and E. coli YidC'"
/codon_start=1
/product="Cox18p"
/protein_id="EWG91038.1"
/translation="MLKRLANRQNGFASFSCSSVGLRYGRTNPSTKRSFSLFQSVADT
FLTVHEASHIPWIVLVPLTTMTLRTLVTLPFSIWQRRRILKQQELRKLVQPITPIIKL
RLAAVTNKKSRNAARISSNGSFMPLQLQNAGVLTPEQITLLAVKETRKRQKKLFKKYN
VPLWKNALLPMVQIPLWVTVSMGIRTLTETQLIESFYPSWFSALGFSSFDLSSPLVAM
PLLAPILVGTLAVLNVELNGRLMFSSSLSSQGIKTISRNSTRVQEAMTSILNVSRLGC
VVMLAMSSQAPFLLSLYWISSQLFSLVQNIILNWIYPYQR"
gene complement(594060..594368)
/gene="Spt4"
/locus_tag="P301_G12111"
mRNA complement(594060..594368)
/gene="Spt4"
/locus_tag="P301_G12111"
/product="Spt4p"
CDS complement(594060..594368)
/gene="Spt4"
/locus_tag="P301_G12111"
/note="Similar to YGR063C 'Protein involved in the
regulating Pol I and Pol II transcription, pre-mRNA
processing, kinetochore function, and gene silencing;
forms a complex with Spt5p'"
/codon_start=1
/product="Spt4p"
/protein_id="EWG91039.1"
/translation="MSSERACMLCGIVQTTNEFNRDGCPNCQGIFEEAGVSTMECTSP
SFEGLVGMCKPTKSWVAKWLSVDHSIAGMYAIKVDGRLPAEVVELLPHYKPRDGSQVE
"
gene complement(594621..596402)
/gene="Vht1"
/locus_tag="P301_G12121"
mRNA complement(594621..596402)
/gene="Vht1"
/locus_tag="P301_G12121"
/product="Vht1p"
CDS complement(594621..596402)
/gene="Vht1"
/locus_tag="P301_G12121"
/note="Similar to YGR065C 'High-affinity plasma membrane
H+-biotin (vitamin H) symporter; mutation results in fatty
acid auxotrophy; 12 transmembrane domain containing major
facilitator subfamily member; mRNA levels negatively
regulated by iron deprivation and biotin'"
/codon_start=1
/product="Vht1p"
/protein_id="EWG91040.1"
/translation="MTISNKSWRSYFPHLRKLPEDDQYLYSDDTNSSIIAEEELHHSV
DKSSKTDVTAETTAVEPHPHNLRHDLPYEVRDEAGRKWWKYFDEFEYRVNKEYKKSRK
WYEFLYPNHTTQTKAERRLLYKLDIIIALYFFMLCWSKSVDLNNYTNAYVSNMKEDLN
MKGNDYVYTSTIANVGAIVFQLPFMYLLPRFPSHIILPVMDLGWTWFTFACYRANSLA
ELRAYRFILSAFGAAYYPVSQYILGCWYAPDEINSRVCLFFCGQQLGSVTSGLLQSRI
FKSLNGVHGLAGWRWMFLIDAIAISLPTAIIGFFVIPGVPSKCYSLFLTDEEIRIARA
RNKRNQIKDGVDKSKLAPLWSRKLWKKVFCTPAFWVLVVFDTCSWNNMTAYSGSYTLW
LKSNTKYSIAQVNNLSVIPACLGFAYVIFCAFGADLFRCKWIFMVFAAIMNTVSCALL
IKWDIPSKAKWYAFFTTYFSVAASPCLWSFINDFLRFDPQVKAITWIAIYSFSQSTYA
WIPTLAWPTVESPRFKTGYTVSLIFGAIYGLWTFVVLFFYKRNEKKHALGNGIILYDS
NKGEELPEFVKKNMEERDGYYYLKRSS"
gene complement(597463..598341)
/locus_tag="P301_G12126"
mRNA complement(597463..598341)
/locus_tag="P301_G12126"
/product="hypothetical protein"
CDS complement(597463..598341)
/locus_tag="P301_G12126"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91041.1"
/translation="MTSLNIMGRKFILERAKRNDNIEEIYTSAYVSLPSSTDTRLPHF
KAKEEDCDVYEEGTNLVGKNAKYTYRSLGRHLDFLRPGLRFGGSQSSKYTYYTVEVKI
DTVNLPLYKDSRSLDPHVTGTFTIKNLTPVLDKVVTLFEGYVINYNQFPLCSLHWPAE
ETLDPYMAQRESDCSHWKRFGHFGSDNWSLTERNFGQYNHESAEFMNQRYIYLKWKER
FLLDDEEQENLMLDDNHHLEGASFEGFYYVCLDQLTGSVEGYYYHPACELFQKLELVP
TNCDALNTYSSGFEIA"
gene complement(598914..601328)
/locus_tag="P301_G12136"
mRNA complement(598914..601328)
/locus_tag="P301_G12136"
/product="hypothetical protein"
CDS complement(598914..601328)
/locus_tag="P301_G12136"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91042.1"
/translation="MAAGQKKYICSFCLKPFSRSEHKIRHERSHAGVKPFQCQVCKHS
FVRRDLLQRHIRTVHRTFLLSSYASMTGDKADIPVSLGVGDVDSTSPRDIKMETLVNS
MIKVNSGLINIHYHSSNVEKMDKQQRCVIGKESSSLKKGKSRFKQVKSRLESSISVKI
LQEYSLDFISSRDILTFFRMGVSHLVENKIFQNFFPDLFSSLQNDELVESFWINKPFG
LIIACLGMSISLNQDSQKLWFICCTNLYASSSKHDNDFDTEDILSQTEQHDVFALILF
YSLLVMLENNIPVSNSIKKFDVFSMLQDILKPFTVASSSYHYLNSKENAWFIFDLWVN
ILRDSNNFNNDSLLIFGWFVNQEFISSNPLKDFIYKGPSMSTTDLTLKHINILADSAY
VYFIIKKTYPQELPSDFRVHDLLVYLNECFVMQQPIKPETSANPSLFANVMNARITDC
KSKSNWLLWETIWFEFINNLTLRNGTTRNIWFIDNFPQVSTSCLLHHSSSFVDETLIT
TNLSIISMLLNLKSFTLASLNPRNIQLITDIVSFQLKLFSSELIASSDVSPSQVSQLL
VNPNVHLMLYFWFDTIYVQRQSYLSSTEKEEFEKVEVFVNDYIITHQKNLVTDLHSIL
FDFWSDSFIAYHILLHAIVSSLRDNILYPYLIYSPHLNDQTKALLTDISNWSCFALQQ
PFRKTSRGSLSGASDMKSFSVASCLPLSPNFLKRDSNCNKILLPPLDIKAIEPISTSN
YTYVNSAPKQQEKEQPLLRATGNNINLVQTIVVPPQVNMESQEFSASSTDNKQSKNIE
IFSQIK"
gene complement(601866..603626)
/locus_tag="P301_G12146"
mRNA complement(601866..603626)
/locus_tag="P301_G12146"
/product="hypothetical protein"
CDS complement(601866..603626)
/locus_tag="P301_G12146"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91043.1"
/translation="MFSLSSLSSSGGHSEQKERERISYFDIRINSPYKDIILIQGSPL
ELSSIPLSGNLVISVKNEIVVKKISLRLVGRFKLEFLQVGRYKKNSSSLASLVKEKRK
IFECYWDNLLVSSKGDVLVGGENAENQHNSSSGRSTSNQDMDTSGNAIFLSKRSLSSP
VFNKIIRRKTHSSHRKILELPENGVTGTPFEGLRENARSRSSSSNTLNNNSHSYSNRD
GSGSSYLFLMKRGNYELPFNTMLPPEVCETIEGLQSGSILYSFEAIIDGRQLWDTDLS
VHTSPHGPIGSTSTSGNGMRTKNKIIIKKFKYLRILRTLSMDNLAMQEEISVGNTWRD
KLQYETSIPSRAVPIGSTTPVKIKIFPFEKNIRLDRIEMALIQYYAMKDSSAQIYDDE
IAVMKITHLADFGPLTDKLDVDCPFTIPDNLKQITQDCCLQDNLIRVMHKLQVRILLQ
RQVDGEYKNLEIKAQLPMLLFISPHLPMKGRLVLFDKHDGKIHFRPGELVPLFLTTYP
AQGLTPGVELNSTTTAHLALPQPPPNYHESTNDHLMPALQPLGADSVALTVPSYEQAQ
AQASASSYVTGSVPAYCDDD"
assembly_gap 603718..604253
/estimated_length=536
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 604348..607814
/gene="Rom1"
/locus_tag="P301_G12161"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(608056..610605)
/locus_tag="P301_G12166"
mRNA complement(608056..610605)
/locus_tag="P301_G12166"
/product="hypothetical protein"
CDS complement(608056..610605)
/locus_tag="P301_G12166"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91044.1"
/translation="MNTALDDLHGDLVTLEDNEIINNSDHSSSHSTSHEEEDEEEDDT
EDIELIEKDGNKILSSRIHPEDEIINDGLNIWIPVQMLKKNIAKFWSHFLAIEKKLTK
VKCKHCGEILTRSDASLTKTFRSHLKTKHNISANKNFYSMNFTVGDSNLKNNTSSTEI
TRRHGYDSLTFNSDQSFKCFDIGKLQSSNYLSISQLVAIVIASENLPLNFFENVSFKS
LLSKFHRIPPLTTNIIEESIIGLSKSIDELIRRSISRNDTQLPFTIHLSDSKESNQPL
YLKYSREIRAQLSNLDLSHLISVNFTELAGKRSLFSLQLFDNTNKVSKGLPLSIFVRK
TTDIDISVWQEQLNNLYSKYPGLQKSVISITLPQSHYTMVLENRNSHNFTFHSGSVRE
IKYHTCIVSELLHCFLQPLFNVPTESMLSSFSVAKENHSGGSLLDSLIDFSHIDLSST
ILGKICCLIEEVNLNDSLKSDFLLYCQNYTQPNCNELTSILSCNCDRFSALKSILEKF
ANLVPFFKSINSHLENESLSESDFRLINTVEETLRTFEQSIEYFASSAPLKFTHTLVF
IIKFELYLTEIIRSFKFTKSKKPFEKILARLLKVKDLYLLDDVNLIGAFLYPSIFQSK
SLLNEIFGTTSVNKIVHNMTKIVLRYLKNFINITNFRSSNSGGESGRNSGNNLLSDYE
AIFMKESRDVELLCNTKLTAPLTEDSLLVQIIRDDLLRYVNRIAHELPNAYHDYLNDN
DISFDGSHFTKHELSEENDSNSGEWCLNPMEETFDIHIPISDSIWNNYISSKNKIEVI
DILLQLLSVNSTSSIRSELSSLTANQDFSTKLSEETIKIKLLNSQFNLRKN"
gene 610846..612010
/gene="Upf3"
/locus_tag="P301_G12171"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 612255..612695
/gene="Smd1"
/locus_tag="P301_G12181"
mRNA 612255..612695
/gene="Smd1"
/locus_tag="P301_G12181"
/product="Smd1p"
CDS 612255..612695
/gene="Smd1"
/locus_tag="P301_G12181"
/note="Similar to YGR074W 'Core Sm protein Sm D1; part of
heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p,
Smx3p, and Smx2p) that is part of the spliceosomal U1, U2,
U4, and U5 snRNPs; homolog of human Sm D1'"
/codon_start=1
/product="Smd1p"
/protein_id="EWG91045.1"
/translation="MKLVNFLKKLRNEQVTIELKNGTTVWGTLQSVSPQMNAILTDVK
LTLPQPRLNKLNSNGIAMASLYLTGGQQPTASDNIASLQYINIRGNTIRQIILPDSLN
LDSLLVDQKQLNSLRRSGQIANDPSKKRRRDFGAPANKRPRRGL"
gene complement(612690..613418)
/gene="Prp38"
/locus_tag="P301_G12186"
mRNA complement(612690..613418)
/gene="Prp38"
/locus_tag="P301_G12186"
/product="Prp38p"
CDS complement(612690..613418)
/gene="Prp38"
/locus_tag="P301_G12186"
/note="Similar to YGR075C 'Unique component of the
U4/U6.U5 tri-snRNP particle, required for conformational
changes which result in the catalytic activation of the
spliceosome; dispensible for spliceosome assembly'"
/codon_start=1
/product="Prp38p"
/protein_id="EWG91046.1"
/translation="MAVNEFQVESNISPKQLNNQSVSLVIPRLTRDKIHNSMYYKVNL
SNESLRGNTMVELLKVMIGAFGTIKGQNGHLHMMVLGGIEFKCILMKLIEIRPNFQQL
NFLLNVKNENGFDSKYIIALLLVYARLQYYYLNGNNKNDDDENDLIKLFKVQLYKYSQ
HYFKLKSFPLQVDCFAHSYNEELCIIHIDELVDWLATQDHIWGIPLGKCQWNKIYNSD
EESSSSESESNGDSEDDNDTSSES"
gene complement(613658..614119)
/gene="Mrpl25"
/locus_tag="P301_G12191"
mRNA complement(613658..614119)
/gene="Mrpl25"
/locus_tag="P301_G12191"
/product="Mrpl25p"
CDS complement(613658..614119)
/gene="Mrpl25"
/locus_tag="P301_G12191"
/note="Similar to YGR076C Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl25p"
/protein_id="EWG91047.1"
/translation="MSYKQYFDSLPLKLKSFFQRYPPSIKYSPVSTSTKAINANPFLP
NKHPVTQRFHDPKYSLRRMSDVYKLALRYGVEEFLPPIENTKKLFFEEKYNKKTLMKG
VLLPKGHKHELKLNEKLKKREEALKKVDELIASKKGSKYAKRVEKMKKTKV"
gene complement(614286..615722)
/gene="Pex8"
/locus_tag="P301_G12196"
mRNA complement(614286..615722)
/gene="Pex8"
/locus_tag="P301_G12196"
/product="Pex8p"
CDS complement(614286..615722)
/gene="Pex8"
/locus_tag="P301_G12196"
/note="Similar to YGR077C Intraperoxisomal organizer of
the peroxisomal import machinery, tightly associated with
the lumenal face of the peroxisomal membrane, essential
for peroxisome biogenesis, binds PTS1-signal receptor
Pex5p"
/codon_start=1
/product="Pex8p"
/protein_id="EWG91048.1"
/translation="MVLDGVLVTYPSFKQLNNAYFIDESSNKTALYYRNWKLQLFSPI
WAQLWNTAIVRANLSIQHCLLIALALLFNQSNRSALLHGVDVSWNLVTEKLLDLLEEY
VHGIVQPMEIFSTDSVLSTNLNHLASCLTGSITRSNEATLVNSVRKLERICRYLSDTV
ASLKEQQLDFKFQNVFILIILALKELSAMNMTILPNHKDTFYSMICLSLFHVHVLTQK
IGTVGFPSYDYVYDNLVTYFIVMDDLSKITTVLELMKRNNTKQDPNKLVFYINFLNKI
TNYYGCRIRLPFITEFIEPLLHFDVFFSGKTGNTLDIEIKESIHTLTITVLSIDSSYS
SQVAQWQVSRILVYLKMSMDQFIAGKLSANQILLIFGHLSTQLPSLHNYNKHLLRDSL
HETYIRIVNVKNPEKKNVLIECLIVQIAFINNPHHLIGWLNICLQLINTHNKKLLQQL
WEMVSSLESSLAIDWWYTTVLSSQSSKL"
assembly_gap 615936..616367
/estimated_length=432
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<616368..616884)
/gene="Pac10"
/locus_tag="P301_G12201"
mRNA complement(<616368..616884)
/gene="Pac10"
/locus_tag="P301_G12201"
/product="Pac10p"
CDS complement(<616368..616884)
/gene="Pac10"
/locus_tag="P301_G12201"
/note="Similar to YGR078C Part of the heteromeric
co-chaperone GimC/prefoldin complex, which promotes
efficient protein folding"
/codon_start=1
/product="Pac10p"
/protein_id="EWG91221.1"
/translation="MDTLFNSTEKNARGIPQAPFIENVNEIIKDPSDFELCFNKFQER
LSKYKFMQESKLATIKQLKTRIPDLENTLKICQSLRNHSDEGDESDEPILLHYQLNDT
LYTKAQVDIPEDRADLKVGLWLGADVMLEYPIDEAIELLKKKLADSEQSLTVSTEDVE
FLRENITTMEVN"
gene 617227..618339
/locus_tag="P301_G12206"
mRNA 617227..618339
/locus_tag="P301_G12206"
/product="hypothetical protein"
CDS 617227..618339
/locus_tag="P301_G12206"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91049.1"
/translation="MSSAANEGCVYLFIVVLRLSSFSCVNSFIHSFTRSRTRSSYSLD
ERSLVSYSIVYVAMNKSSKAFQVPNKVITKEDITPLSRSHTKKADTRGTADGKNTTAS
AVEATPIIITTARSIDTAGSLSENATEDDGTQNGDLHDDDDDDDLESTLGYSSEPDPL
FSPCHQPSFTNSTFSYSADNELPMEENHDKNNFHDSSESSIFLPQIQQSFFFGDNSKS
DANNTDFWKEVNGTAEEAICLQETRQRKCSLVALHPGDATTSSNDTLGIEDFIKDDIN
SAEAMEPSPSSSPSSSLLDNLDYNIKLLCYRDNEGKFTLKKRKFLKNSLRSSSAISKK
WKPLSKRDKLLKRAIRRKSGVCQTLSAGFGIGEFML"
gene 618517..619515
/gene="Twf1"
/locus_tag="P301_G12211"
mRNA 618517..619515
/gene="Twf1"
/locus_tag="P301_G12211"
/product="Twf1p"
CDS 618517..619515
/gene="Twf1"
/locus_tag="P301_G12211"
/note="Similar to YGR080W Twinfilin, highly conserved
actin monomer-sequestering protein involved in regulation
of the cortical actin cytoskeleton, composed of two
cofilin-like regions, localizes actin monomers to sites of
rapid filament assembly"
/codon_start=1
/product="Twf1p"
/protein_id="EWG91050.1"
/translation="MSTQSGIVAEQALLHSLNENLSADGIVIIIAKISPDSTSVHQTQ
VARSFEELVQLASQEREPLYIFYKPEGLDKYFFVSFIPDGSPVRSRMLYASTKNTLAR
QVGSNSLSTEQPLITDAQDLVDLKNFDSARPAGQNKPLTHDEEMQIEINKQQALLRKN
TSVKLVSQDSASPLSLTFRVNSEKPINEILDSEGKNLIIFQIDPSNETIQIVQSDTCP
SVDELYIDLPGPSYTIFRQGDSSFFIYSCPSGSKVKDRMIYASNKNGFINYLKNDQKI
AFSKVVEIGDFVELDKSLLMATNKEDSLDHGSNPDLPNKSNLKFNKPKGPLRKRRT"
gene complement(619655..620287)
/gene="Slx9"
/locus_tag="P301_G12216"
mRNA complement(619655..620287)
/gene="Slx9"
/locus_tag="P301_G12216"
/product="Slx9p"
CDS complement(619655..620287)
/gene="Slx9"
/locus_tag="P301_G12216"
/note="Similar to YGR081C 'Protein required for pre-rRNA
processing; associated with the 90S pre-ribosome and 43S
small ribosomal subunit precursor; interacts with U3
snoRNA; deletion mutant has synthetic fitness defect with
an sgs1 deletion mutant'"
/codon_start=1
/product="Slx9p"
/protein_id="EWG91051.1"
/translation="MVAKKRNTLRSKVSARNSQNFGPDVANNGILDESYDIESDPRAF
LHQPKETKKEKLLNRQNTFLSNLKGKSTLNDGIAANFDGISKSSIRRRKRKLREELKP
RMQDLLTSLEQEKDLRGIIENSSKDMNNDDDIDMDSKIRFVDTKEMNLKKIEPGSVRI
KKNQPNIRNQKGAKALAANETARFNQVLTNQDFQKNPFGALREVIKLQKQ"
gene 620551..621102
/gene="Tom20"
/locus_tag="P301_G12221"
mRNA 620551..621102
/gene="Tom20"
/locus_tag="P301_G12221"
/product="Tom20p"
CDS 620551..621102
/gene="Tom20"
/locus_tag="P301_G12221"
/note="Similar to YGR082W 'Component of the TOM
(translocase of outer membrane) complex responsible for
recognition and initial import steps for all
mitochondrially directed proteins; acts as a receptor for
incoming precursor proteins'"
/codon_start=1
/product="Tom20p"
/protein_id="EWG91052.1"
/translation="MSQSNPILRGLAITTAIAALSATGYAIYFDYQRRNSPQFRKVLR
QRAKEQAKMEEQAKTHAKEVKLQKVTEFLSMELAKDPIPSDPSEREATFTTNVENGER
LSMQQGKELEAASKFYKALTVYPQPADLLGIYQRSIPEAIYEYIILMIAILPPANVAS
FVKGVVGSKAESDAVAEANDIDD"
gene complement(621365..623320)
/gene="Gcd2"
/locus_tag="P301_G12226"
mRNA complement(621365..623320)
/gene="Gcd2"
/locus_tag="P301_G12226"
/product="Gcd2p"
CDS complement(621365..623320)
/gene="Gcd2"
/locus_tag="P301_G12226"
/note="Similar to YGR083C 'Delta subunit of the
translation initiation factor eIF2B, the
guanine-nucleotide exchange factor for eIF2; activity
subsequently regulated by phosphorylated eIF2; first
identified as a negative regulator of GCN4 expression'"
/codon_start=1
/product="Gcd2p"
/protein_id="EWG91053.1"
/translation="MSESEAKSRSATPPSKAKQATPTTTAAANGEKKLTNKELKELKK
QEKAAKRAAMKQANGISIEQQQQQAQMKKEKKQLQREQQQKREQKQKNANKKKQNERN
VKKSTLFGHLETTEERRATILALTSAVSSPKTSRITAAGLMVPVVASALSGSNVLTAS
SLMPVGPNASSTVSASAPASTTTTLPASSAALSAGTSSASTNTPTAIQQEIASSNASD
VAKTLASISLEAGEFNVIPGISSVIPTVLEQSFDNSSLISSVKELLLNKDLIHPSILL
LTSHLAHYKIVGSIPRCIAMLEVFQIVIKDYQTPKGTTLSRNLTSYLSHQIDLLKKAR
PLSVTMGNAIRWLKQEISLIDPSTPDKAAKKDLCEKIGQFAKEKIELADQLIIDNAST
QIEESTTIVTYGSSKVLTELLLHNAISLKKNIKVIVVDSRPLFEGRKMAETLRNAGVN
VMYALITSLDTIFNMDVDYVFLGAHSILSNGFLYSRAGTAMLAMSAKRRNIPVLVCCE
SLKFSQRVQLDSVTFNELADPNDLVNIDYENPVERRGNKGALLNQFIKERKFEKKKLA
MENKPKGNKIGGKKGSEGESKDASNEEDSNSKNILDGWQELPSLNIVNILYDLTPPEY
IKKVITEFGALPPSSVPVILREYKGSA"
gene complement(623628..624647)
/gene="Mrp13"
/locus_tag="P301_G12231"
mRNA complement(623628..624647)
/gene="Mrp13"
/locus_tag="P301_G12231"
/product="Mrp13p"
CDS complement(623628..624647)
/gene="Mrp13"
/locus_tag="P301_G12231"
/note="Similar to YGR084C Mitochondrial ribosomal protein
of the small subunit"
/codon_start=1
/product="Mrp13p"
/protein_id="EWG91054.1"
/translation="MGTITVVINEGPILLIRALHRATTNKKMFKSTVWRRFASTGEIA
KAKLDEFLIYHKTDAKLKPFIYRPKNAQILLTKDIRDPKTREPLQPRPPVKPLSKQTL
NDFIYSVEPNSTELLDWFKEWTGTSIRKRAIWTYISPIHVQKMLTASFFKIGKYAHMV
GLLYGIEHKFLKAQNPSVFDIEHFFNTNIMCALHRNRLKDYKDAEIAQRKLQVAWKKV
LNRKNNTGLANILVATLGRQIGFTPELTGLQPVDISLPDIPNSSSGAELKDLLSKYEG
IYLIARTLLDIDQHNAQYLELQEFIRQYQNALSESSDPYDTHLKALGLLETPPPQEST
EKEEK"
gene complement(624888..625412)
/gene="Rpl11b"
/locus_tag="P301_G12236"
mRNA complement(624888..625412)
/gene="Rpl11b"
/locus_tag="P301_G12236"
/product="Rpl11bp"
CDS complement(624888..625412)
/gene="Rpl11b"
/locus_tag="P301_G12236"
/note="Similar to YGR085C 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl11Ap;
involved in ribosomal assembly; depletion causes
degradation of proteins and RNA of the 60S subunit; has
similarity to E. coli L5 and rat L11'"
/codon_start=1
/product="Rpl11bp"
/protein_id="EWG91055.1"
/translation="MSTKAQNPMRDLKIEKLVLNISVGESGDRLTRASKVLEQLSGQT
PVQSKARYTVRTFGIRRNEKIAVHVTVRGPKAEEILERGLKVKEYQLRDRNFSATGNF
GFGIDEHIDLGIKYDPSIGIFGMDFYVVMNRPGARVTRRKRCKGTVGNSHKTTKEDTV
SWFKQKYDADVLDK"
gene complement(626099..627118)
/gene="Pil1"
/locus_tag="P301_G12241"
mRNA complement(626099..627118)
/gene="Pil1"
/locus_tag="P301_G12241"
/product="Pil1p"
CDS complement(626099..627118)
/gene="Pil1"
/locus_tag="P301_G12241"
/note="Similar to YGR086C 'Primary component of eisosomes,
which are large immobile cell cortex structures associated
with endocytosis; null mutants show activation of
Pkc1p/Ypk1p stress resistance pathways; detected in
phosphorylated state in mitochondria'"
/codon_start=1
/product="Pil1p"
/protein_id="EWG91056.1"
/translation="MHRTYSLRNSRAPTASQLQNPPPPPSTTKGRFFGKGGLAYSFRR
SAAGAFGPELSRKLSQLVKIEKNVLRSMELTANERRDAAKQLSIWGLENDDDVSDITD
KLGVLIYEVSELDDQFIDRYDQYRLTLKSIRDIEGSVQPSRDRKDKITDKIAYLKYKD
PQSPKIEVLEQELVRAEAESLVAEAQLSNITRSKLRAAFNYQFDSIIEHSEKIALIAG
YGKALLELLDDSPVTPGETRPAYDGYEASKQIIIDAESALNEWTLDSAQVKPTLSFKQ
DYEDFEPEEGEEEEEEDGQGRWSEDEQEDGQIEEPEQEEEGAVEEHEQVGHQQSESLP
QQTTA"
gene complement(627790..629481)
/gene="Pdc6"
/locus_tag="P301_G12251"
mRNA complement(627790..629481)
/gene="Pdc6"
/locus_tag="P301_G12251"
/product="Pdc6p"
CDS complement(627790..629481)
/gene="Pdc6"
/locus_tag="P301_G12251"
/note="Similar to YGR087C 'Minor isoform of pyruvate
decarboxylase, decarboxylates pyruvate to acetaldehyde,
involved in amino acid catabolism; transcription is
glucose- and ethanol-dependent, and is strongly induced
during sulfur limitation'"
/codon_start=1
/product="Pdc6p"
/protein_id="EWG91057.1"
/translation="MSEITLGKYLFERLKQVNVNTIFGLPGDFNLSLLDKIYEVDGLR
WAGNANELNAAYAADGYARIKGLSVLVTTFGVGELSALNGIAGSYAEHVGVLHVVGVP
SISAQAKQLLLHHTLGNGDFTVFHRMSANISETTSMITDIATAPSEIDRLIRTTFITQ
RPSYLGLPANLVDLKVPGSLLEKPIDLSLKPNDPEAEKEVIDTVLELIQNSKNPVILS
DACASRHNVKKETQKLIDLTQFPAFVTPLGKGSIDEQHPRYGGVYVGTLSKQDVKQAV
ESADLILSVGALLSDFNTGSFSYSYKTKNVVEFHSDYVKVKNATFLGVQMKFALQNLL
KVIPDVVKGYKSVPVPTKTPANKGVPASTPLKQEWLWNELSKFLQEGDVIISETGTSA
FGINQTIFPKDAYGISQVLWGSIGFTTGATLGAAFAAEEIDPNKRVILFIGDGSLQLT
VQEISTMIRWGLKPYLFVLNNDGYTIEKLIHGPHAEYNEIQTWDHLALLPAFGAKKYE
NHKIATTGEWDALTTDSEFQKNSVIRLIELKLPVFDAPESLIKQAQLTAATNAKQ"
gene 631124..632812
/gene="Ctt1"
/locus_tag="P301_G12256"
mRNA 631124..632812
/gene="Ctt1"
/locus_tag="P301_G12256"
/product="Ctt1p"
CDS 631124..632812
/gene="Ctt1"
/locus_tag="P301_G12256"
/note="Similar to YGR088W Cytosolic catalase T, has a role
in protection from oxidative damage by hydrogen peroxide"
/codon_start=1
/product="Ctt1p"
/protein_id="EWG91058.1"
/translation="MNVFGKKEEKQEKVYSLQNGFPYSHHPYASQYSRPDGPILLQDF
HLLENIASFDRERVPERVVHAKGGGCRLEFELTDSLSDITYAAPYQNVGYKCPGLVRF
STVGGESGTPDTARDPRGVSFKFYTEWGNHDWVFNNTPVFFLRDAIKFPVFIHSQKRD
PQSHLNQFQDTTIYWDYLTLNPESIHQITYMFGDRGTPASWASMNAYSGHSFIMVNKE
GKDTYVQFHVLSDTGFETLTGDKAAELSGSHPDYNQAKLFTQLQNGEKPKFNCYVQTM
TPEQATKFRYSVNDLTKIWPHKEFPLRKFGTITLTENVDNYFQEIEQVAFSPTNTCIP
GIKPSNDSVLQARLFSYPDTQRHRLGANYQQLPVNRPRNLGCPYSKGDSQYTAEQCPF
KAVNFQRDGPMSYYNFGPEPNYISSLPNQTLKFKNEDNDEVSDKFKGIVLDEVTEVSV
RKQEQDQIRNEHIVDAKINQYYYVYGISPLDFEQPRALYEKVYNDEQKKLFVHNVVCH
ACKIKDPKVKKRVTQYFGLLNEDLGKVIAEGLGVPWEPVDLEGYAKTWSIASAN"
gene 633455..636265
/gene="Nnf2"
/locus_tag="P301_G12261"
mRNA 633455..636265
/gene="Nnf2"
/locus_tag="P301_G12261"
/product="Nnf2p"
CDS 633455..636265
/gene="Nnf2"
/locus_tag="P301_G12261"
/note="Similar to YGR089W 'Protein that exhibits physical
and genetic interactions with Rpb8p, which is a subunit of
RNA polymerases I, II, and III; computational analysis of
large-scale protein-protein interaction data suggests a
role in chromosome segregation'"
/codon_start=1
/product="Nnf2p"
/protein_id="EWG91059.1"
/translation="MEEQFTNQKKVSHLQSLMNTKRSEQPTEFAKKHRFKDTLALFLV
FLSFNHFTSLCLLVSFIVATKCKDFLANCFIILFLSKKPSRHIGEVAHIDISTSKVTN
GSSNRKSNSRFFGNSKNSFVIPIPVLICEILFAMLLKIYGGNYFVKPIKNLAISIIAS
FLINDPSDCLSYATSCSVLYAVSTNTFQRVSHFFDIIQLFDMSLRGTGQSIKLFTVFR
KYSQFFKKLFSLFLPMPFKMLGKHSDSMIYYLSFHILFFSFASSLLHPHRQTAENKPL
KKGFNSTKPDVARVQGLQKMRISSSSSVSADSNTLEDQSPMIPNDPGGLSSSNQTIHP
SQQNNSPVPLSSHSNILNPAASYPTDATSSFPYFTSMVKEYKSYQPSVISAEGSNSQA
VTTTTSTTTSPTTFNFSGDNTSLSNEISLSDSSNGNSKKDSDFFSPSNDKYTNQLFEL
NVDFGNMFSSSKLSSDISVTSNLENFIRLLFRRKNQHLIAPLWSMVVTLKTTNFEKKY
LQETSENSLTPTNSNTSYVSNQEKHDKDLDTINTHSVSSRISFTHAGKFKKSVFNNFE
PSNTMALIAKTTSDDYNLLNLVSTNENIFNRNDNDYKVCIIDISTNSITFHIENLHDG
ELIVLVNGVIWSEVSCALILEHVGEEYVVVNGLVPSCSYDIQFINRLNHRDDYLVSDL
IVRTCGNNNAIAGKFENLDFSFPSYYHRKFLSPLLTLKHSVLTTNANLSDERTKLKKT
KKEFSKKLSLLRQEIDYFKGRISQNATHDEKSTLKVENLKVALQQSETAVNKLEMQLK
TLTEKELELEEEYLKKKDLHLKNQLEFSKLEESLSKDLKNSEGRFQKVNQELVQLGSK
LDKLNARNEKLQKEVDQNAEEIEKFSTQFLSKREKDRFRRKEYRIREANKFELTIKGL
EQDINRLENENENIHSLIGNSY"
assembly_gap 636394..636658
/estimated_length=265
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 637995..638096
/estimated_length=102
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 638244..638315
/locus_tag="P301_G12276"
tRNA 638244..638315
/locus_tag="P301_G12276"
/product="tRNA-Thr"
/note="TT(UGU)G1"
gene 639638..642571
/gene="Utp22"
/locus_tag="P301_G12281"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 639724..639892
/estimated_length=169
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 642840..644324
/gene="Prp31"
/locus_tag="P301_G12286"
mRNA 642840..644324
/gene="Prp31"
/locus_tag="P301_G12286"
/product="Prp31p"
CDS 642840..644324
/gene="Prp31"
/locus_tag="P301_G12286"
/note="Similar to YGR091W Splicing factor, component of
the U4/U6-U5 snRNP complex"
/codon_start=1
/product="Prp31p"
/protein_id="EWG91060.1"
/translation="MSSEEDYFDELEYDLADEVNEEKEDIQTKKLTTVNCQTEKFNPF
EILPESIELFRTLALISPDRLSLSETAQILPKIVDLKRILQQQEIDFIKLLPFFNEII
PLIKSNIKLMHNFLISLYSRRFPELSSLIPSPLQYSKVISILENENYSKNESDELFFH
LENKAKLTREQILVLTMSMKTSFKNKEPLDIKTRTQILEANSILENLWKLQEDIGQYI
ASKISIIAPNVCFLVGPEIAAQLIAHAGGVLEFSRIPSCNIASIGKNKHLSHELHTLE
SGVRQEGYLFASDMIQKFPVSVHKQMLRMLCAKVSLAARVDAGQKNGDRNTVLAHKWK
AELSKKARKLSEAPSISETKALPIPEDQPKKKRAGRKFRKYKEKFRLSHVRQLQNRME
FGKQEQTVLDSYGEEVGLGMSNTSLQQAVGATSGSRRSAGNQAKLTKVMKHRISEANQ
QADEFLISLGHNTEQPNLSPEMVQMHKKQHTNPEEETNWFSGHG"
gene 644688..646406
/gene="Dbf2"
/locus_tag="P301_G12291"
mRNA 644688..646406
/gene="Dbf2"
/locus_tag="P301_G12291"
/product="Dbf2p"
CDS 644688..646406
/gene="Dbf2"
/locus_tag="P301_G12291"
/note="Similar to YGR092W 'Ser/Thr kinase involved in
transcription and stress response; functions as part of a
network of genes in exit from mitosis; localization is
cell cycle regulated; activated by Cdc15p during the exit
from mitosis'"
/codon_start=1
/product="Dbf2p"
/protein_id="EWG91061.1"
/translation="MLSKSEKNVDLLAGNMSNLSFDGHGTPGGTGLFPNQNITKRRTR
PAGINDSPSPVKPSFFPYEDTSNMDIDEVSQPDMDVSNSPKKLPPKFYERATSNKTQR
VVSVCKMYFLEYYCDMFDYVISRRQRTKQVLEYLQQQSQLPNSDQIKLNEEWSSYLQR
EHQVLRKRRLKPKNRDFEMITQVGQGGYGQVYLARKKDTKEVCALKILNKKLLFKLNE
TKHVLTERDILTTTRSEWLVKLLYAFQDLQSLYLAMEFVPGGDFRTLLINTRCLKSGH
ARFYISEMFCAVNALHDLGYTHRDLKPENFLIDAKGHIKLTDFGLAAGTISNERIESM
KIRLEKIKDLEFPAFTEKSIEDRRKMYNQLREKEINYANSMVGSPDYMALEVLEGKKY
DFTVDYWSLGCMLFESLVGYTPFSGSSTNETYDNLRRWKQTLRRPRQSDGRAAFSDRT
WDLITRLIADPINRLRSFEHVKRMSYFADINFSTLRSMIPPFTPQLDSETDAGYFDDF
TSEADMAKYADVFKRQDKLTAMVDDSAVSSKLVGFTFRHRNGKQGSSGILFNGLEHSD
PFSTFY"
gene 646887..648410
/gene="Drn1"
/locus_tag="P301_G12301"
mRNA 646887..648410
/gene="Drn1"
/locus_tag="P301_G12301"
/product="Drn1p"
CDS 646887..648410
/gene="Drn1"
/locus_tag="P301_G12301"
/note="Similar to YGR093W 'Putative protein of unconfirmed
function; green fluorescent protein (GFP)-fusion protein
localizes to the nucleus'"
/codon_start=1
/product="Drn1p"
/protein_id="EWG91062.1"
/translation="MTNAKILVAHISESDADEAIRKIKKVNEKSGPFDLIIIFSNSYD
ENFELNTDGLPQLILLSCDKANNSKSKKINENVTLLHNMGTYKLANGITLSYFIYPDD
TLQGEKKSILDEFGKSEDQVDILLTKEWGLSISERCGRLSGSEVVDELAKKLQARYHF
AFSDEINFYELEPFQWERERLSRFLNIPKYGSGKKWAYAFNMPIGDNELKDEPEPPNL
IANPYNSVVTNSNKRPLETETENSFDGDKQVLANREKNENKKIRTILPSSCHFCFSNP
NLEDHMIISIGKLVYLTTAKGPLSVPKGDMDISGHCLIIPIEHIPKLDPSKNAELTQS
ILAYEASLVKMNYIKFDMCTIVFEIQSERSIHFHKQVIPVPKYLVLKFCSALDRQVHF
NNEKFTRNAKLEFQCYDSHSSKQYVDVINNQSNNYLQFTVYETPEADPKIYLATFNAS
ETIDLQFGRRVLAFLLNLPRRVKWNSSTCLQTKQQETIEAEKFQKAYRTYDISLTEN"
gene 648685..651981
/gene="Vas1"
/locus_tag="P301_G12306"
mRNA 648685..651981
/gene="Vas1"
/locus_tag="P301_G12306"
/product="Vas1p"
CDS 648685..651981
/gene="Vas1"
/locus_tag="P301_G12306"
/note="Similar to YGR094W Mitochondrial and cytoplasmic
valyl-tRNA synthetase"
/codon_start=1
/product="Vas1p"
/protein_id="EWG91063.1"
/translation="MNKWLNTLSKTFTFRLLNCHYRRSLPLCQNFSLKKSLTHNQVRF
FKMSDLDNLPPVDPKTGEVIINPLKEDGSPKTPKEIEKEKKKAEKLLKFAAKQAKKNA
AATTGASQKKPKKKKEVEPIPEFIDKTVPGEKKILVSLDDPALKAYNPANVESSWYDW
WIKTGVFEPEFTADGKVKPEGVFCIPAPPPNVTGALHIGHALTIAIQDSLIRYNRMKG
KTVLFLPGFDHAGIATQSVVEKQIWAKDRKTRHDYGREAFVGKVWEWKEEYHSRIKNQ
IQKLGASYDWSREAFTLSPELTKSVEEAFVRLHDEGVIYRASRLVNWSVKLNTAISNL
EVENKDVKSRTLLSVPGYDEKVEFGVLTSFAYPVIGSDEKLIIATTRPETIFGDTAVA
VHPDDDRYKHLHGKFIQHPFLPRKIPIITDKEAVDMEFGTGAVKITPAHDQNDYNTGK
RHNLEFINILTDDGLLNEECGPEWQGMKRFDARKKVIEQLKEKNLYVGQEDNEMTIPT
CSRSGDIIEPLLKPQWWVSQSEMAKDAIKVVRDGQITITPKSSEAEYFHWLGNIQDWC
ISRQLWWGHRCPVYFINIEGEEHDRIDGDYWVAGRSMEEAEKKAAAKYPNSKFTLEQD
EDVLDTWFSSGLWPFSTLGWPEKTKDMETFYPFSMLETGWDILFFWVTRMILLGLKLT
GSVPFKEVFCHSLVRDAQGRKMSKSLGNVIDPLDVITGIKLDDLHAKLLQGNLDPREV
EKAKIGQKESYPNGIPQCGTDAMRFALCAYTTGGRDINLDILRVEGYRKFCNKIYQAT
KFALMRLGDDYQPPATEGLSGNESLVEKWILHKLTETSKIVNEALDKRDFLTSTSSIY
EFWYLICDVYIENSKYLIQEGSAIEKKSAKDTLYILLDNALKLIHPFMPFISEEMWQR
LPKRSTEKAASIVKASYPVYVSEYDDVKSANAYDLVLNITKEARSLLSEYNILKNGKV
FVESNHEEYFKTAEDQKDSIVSLIKAIDEVTVVRDASEIPEGCVLQSVNPEVNVHLLV
KGHVDIDAEIAKVQKKLEKAKKSKNGIXXXXXXXXXXXXXXXXXXXXXKSKLITLLPK
SKVWRLLLKT"
assembly_gap 651857..651918
/estimated_length=62
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(652169..652840)
/gene="Rrp46"
/locus_tag="P301_G12311"
mRNA complement(652169..652840)
/gene="Rrp46"
/locus_tag="P301_G12311"
/product="Rrp46p"
CDS complement(652169..652840)
/gene="Rrp46"
/locus_tag="P301_G12311"
/note="Similar to YGR095C 'Exosome non-catalytic core
component; involved in 3'-5' RNA processing and
degradation in both the nucleus and the cytoplasm; has
similarity to E. coli RNase PH and to human hRrp46p
(EXOSC5)'"
/codon_start=1
/product="Rrp46p"
/protein_id="EWG91064.1"
/translation="MSVQAEIGILDHVDGSSEFVSQDTKVICSVTGPIEPKARQELPT
QLALEIIVRPAKGVATTREKVLEDKLRAVLTPLITRHCYPRQLCQITCQILESGEDEA
EFSLRELSCCINAAFLALVDAGIALNSMCASIPIAIIKDTSDIIVDPTAEQLKISLSV
HTLALEFVNGGKVVKNVLLLDSNGDFNEDQLFSLLELGEQKCQELVTNIRRIIQDNIS
PRLVV"
gene 653118..654062
/gene="Tpc1"
/locus_tag="P301_G12321"
mRNA 653118..654062
/gene="Tpc1"
/locus_tag="P301_G12321"
/product="Tpc1p"
CDS 653118..654062
/gene="Tpc1"
/locus_tag="P301_G12321"
/note="Similar to YGR096W 'Mitochondrial membrane
transporter that mediates uptake of the essential cofactor
thiamine pyrophosphate (ThPP) into mitochondria;
expression appears to be regulated by carbon source;
member of the mitochondrial carrier family'"
/codon_start=1
/product="Tpc1p"
/protein_id="EWG91065.1"
/translation="MFKEEDSLRKGQNVAAWKTLLAGAVSGLLARSITAPMDTIKIRL
QLTPANGLKPFGSQVMEVARSMIKNEGIRSFWKGNIPGSLLYVTYGSAQFSSYSLFNR
YLTPFGLEARLHSLVVGAFAGITSSIVSYPFDVLRTRLVANNQMHSMSITREVRDIWK
LEGLPGFFKGSIASMTTITLTASIMFGTYETIRIYCDENEKTTAAHKKWELATLNHSA
GTIGGVIAKIITFPLETIRRRMQFMNSKHLEKFSRHSSVYGSYKGYGFARIGLQILKQ
EGVSSLYRGILVALSKTIPTTFVSFWGYETAIHYLRMY"
gene 655189..658629
/gene="Ask10"
/locus_tag="P301_G12331"
mRNA 655189..658629
/gene="Ask10"
/locus_tag="P301_G12331"
/product="Ask10p"
CDS 655189..658629
/gene="Ask10"
/locus_tag="P301_G12331"
/note="Similar to YGR097W 'Component of RNA polymerase II
holoenzyme, phosphorylated in response to oxidative
stress; has a role in destruction of Ssn8p; proposed to
function in activation of the glycerol channel Fps1p;
paralogous to Rgc1p'"
/codon_start=1
/product="Ask10p"
/protein_id="EWG91066.1"
/translation="MSDYFSSRPSQTLTPMGNKPSGGGGGDDASSIHSKSSQYLMDIL
PDSMTLNESVSSIVANNQAKEFILPETDERSPYFINVPIPKAQPTSTTETKKPLAGDE
AIDGQFVKEYPTDILVDRFYKWKKILKGLVIYLREVAYAQEQFARINYQLKGSVKFPF
LTDIDETTNTITDPFTTAPRGPKKAQPAQKKVGLTDSEQFQMQMQQEQQENAVQVPTD
ESKMSLAPHEYKPVQTTESDNTSAASGFVKFGSGSIQDIQVILKKYHLSLANQQFKIS
KEITSTVIPKLEELRKDLRYKITEIKDLHGDFKTNIGAHIQLTSQLLKKYIAAVKFMN
AHGIGNDRASPTNKKPHKLDPKHDPYLLKLQLDLQLKRQVAEETYLQEAFINLQSSGL
QLEKIIYTKIQHALLRYSALIDSEARLMIKNMCQELQHGIISKPPAFEWDNFVTQHPS
CLLNWKSNDPIPPPRKVSDVIYPHMKSPLAKCIKAGYFLKKSELLPTYHQGYFVLTSN
YIHEFQSSDFYNLSSSTPNSTKSSAYSSSVSIADTYANANNAKANNHHRQASDVHNSS
TTTGGTAGANGIRGIRKKSYLAPIMSIPLNDCTLKDASSTKFVLVGKPTLNENADVRK
SSSSTYLSGSSQASLPKYGHETAKIFSKAPFHKFLKGSKPKNKNTKSSELDQFYAAAQ
KESNNYVTWTFKIVSPEPSEEELKHFKRWVQDLKNLTSFNDTKDRIKFIEDRVMKSHR
FKAGHMSRNSVNIGSHTPCLTDSTFTLQDGTTTSVNLKGRAEKPQYIHIQNNSLADFD
GNGFRSKVNTPAIDDYGNLITVERRPAQSPHQYSDYMATSGNTTPSYSSGSRPQSMYN
GYNPAVSITSNGMMLQQSTANNNTNPTTNLRHQRNISQTSSLPGFSYTSLSLPVNSPG
SSNSESSSGGYFAIPLHGNNNNNNYTQRNSEGSSPCYNDDQIRQQQQPLQMQPLSRTS
SSSVNVTAMRSTSAGNSITANAPVVPKVMVNNQNVKTVAADQSATAPSSPTMNSSVTT
INRESPYQTLKKTNSTGNVPCLTAEKTHAHPAFYKRGNNSAQNLTTSSSTASRVHPIR
KHKKNVSFSSLNSLMFSKKGANHGGNLMTNQFMSGGIQEDDGDSTNNDTIKLNQSIYS
"
assembly_gap 658759..659006
/estimated_length=248
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(659062..663957)
/gene="Esp1"
/locus_tag="P301_G12336"
mRNA complement(659062..663957)
/gene="Esp1"
/locus_tag="P301_G12336"
/product="Esp1p"
CDS complement(659062..663957)
/gene="Esp1"
/locus_tag="P301_G12336"
/note="Similar to YGR098C 'Separase, a caspase-like
cysteine protease that promotes sister chromatid
separation by mediating dissociation of the cohesin Scc1p
from chromatin; inhibits protein phosphatase 2A-Cdc55p to
promote mitotic exit; inhibited by Pds1p'"
/codon_start=1
/product="Esp1p"
/protein_id="EWG91067.1"
/translation="MMVKQEEPLNEISPNTPMTSKSYLLNDTLSKVHHSGQTRPLTSV
LSGDASSNSIGILAMHNNIIRDFTKIASNNIDLAIEDITTVDHSLNSIYSLLKSHHMW
GHINSTVKQHLMIIVKLINNNALGLASSEIIFLFNETNLFQAHSLKNILLADFSTWND
YYLSNLKILALQIILKRKLVDEYLPHILELFSHDKRYLLKDPNLKAHALTKIVLSFFS
VTTSCKVLFGLKFLQYIKQFKLPFKKFISNITVECFSKNLLHKNYLEMGPNKIYLNSF
YLSYSMLYDGLDXXXXXXXXXXXXXXXXXXXXXXXXXXXXXCNMSENRLLWSCISVDD
LNVILENATNFLQNKGKHISATLKCLVCLWSTIRLEGLPKNKDILRQFDCTVIYINSN
IKSINDESAAALLSELLGVLSEICIDYKEPKRLSNIISVLFNASVLFKSHSFLLKTAN
LEISNVLISNDSKTSHRTILKFEKFISSAQSAQKKIEIFSCLFNVYCMLRNDTLSFVF
DFCQNAFIHCFTRLKNHKNSSSFSNSSEIMLSVLYGNSSMENIPSENWSQLSRMIFCS
LRGIFDLDPLELNNTFDKLHLLNKYELLIRIVYLLNLDMSKHLTTNLSKITKLYINKW
LQKSDEKAERISSFEMDFVKMLLCYLNFNNFDKLSIELSLCIKSKEKYYSSIVPYADN
YLLEAYLSLYMIDDALMMKNQLQKTMNLSTAKIEQALLHASSLINVHLWDSDLTAFQI
YFGKTLPAMKPELFDINNDHNLPMSLYIKVILLNIKIFNESAKLNIKAGNVISAVIDC
RKAQNLALSLLKKKNKLSQGSRLALLKSLSFSFFQLIKIHIRIGSARDCEFYSKELSR
IISDLEEPIIVYRCLHFLHRYYMITEQTCLQNITLGKANKAFDYLDAEADITSLTMFL
YDNKEFVKLEQSLVLYFGDQLEKTFLPNLWKLHLGKDIDDSICLSEYMPKNVINRVHN
MWQKVMSQLEEDPFFKGMFESTLGIPSSLPVIPSTMPNNILKTPSKHSTGLKLCDSPR
SSSMTPRGKNIRQKFDRIAAISKLKQMKELLESLKLDTLDNHELSKISSLSSLTLTIL
SNITSIHNAESSLITNFSLTDLPRHMPLLFDKVLNNIDNKNYREFRVSSLIAPNNIST
ITESIRVSAAQKDLMESNLNINVITIDFCPITGNLLLSKLEPRRKRRTHLRLPLIRSN
SRDLDEVHLSFPEATKKLLSIINESNQTTSVEVTNKIKTREERKSWWTTRYDLDKRMQ
QLLNNIENSWFNGVQGFFSPEVVDNSLFEKFKDKFYEILHQNLPSRKLYGNPAMFIKV
EDWVIELFLKLNPQEIDFLSKMEDLIYFVLDILLFHGEENAYDEIDFSMLHVQLEEQI
KKYRATMTTNSIFHTFLVVSSSCHLFPWECLSFLKDLSITRVPSYVCLNKLLSRFHYQ
LPLQVTIEDNISMILNPNGDLSRTESKFKGMFQKIIDAKPSSQLVMNEKPEEETLLKM
LQNSNLFVYIGHGGGEQYVRSKEIKKCTKIAPSFLLGCSSAAMKYYGKLEPTGTIYTY
LLGGCPMVLGNLWDVTDKDIDKFSEELFEKMGFRCNTDDLNGNSLSVSYAVSKSRDVC
HLRYLNGAAPVIYGLPIKFVS"
assembly_gap 663009..663091
/estimated_length=83
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 664398..666464
/gene="Tel2"
/locus_tag="P301_G12341"
mRNA 664398..666464
/gene="Tel2"
/locus_tag="P301_G12341"
/product="Tel2p"
CDS 664398..666464
/gene="Tel2"
/locus_tag="P301_G12341"
/note="Similar to YGR099W Essential DNA-binding protein
specific to single-stranded yeast telomeric DNA repeats,
required for telomere length regulation and telomere
position effect"
/codon_start=1
/product="Tel2p"
/protein_id="EWG91068.1"
/translation="MVLETLKQGLDSSQIHEALIQLDSYPREPVDLDASMVLIKFVIP
VYPSLPERSKVILRRLASKSFTFLCQIVTFSRTISGRDGLQEIRIYQEILEDIISFEP
GCLTFYLKASTTSKADRDSIKALFFGSKLFNVLANRIDMAKYLGYLRLQWKFLLESNE
TDPPGFLGEWLVSSFLLNPVLAADMLLGELFLLKESYFFSFQKIISASSLIDQKRLIA
KFLLPYIQVIVTLENLNDVRKILRRFDLDKIISLSVLFEIQSLPLKEVIVRLMSNHSS
TKFVSALVSKFADFTDEEVDTKTCELLVLFAVHNLNHSQREEIAHDERFLNGVTKHLG
SNEREARERAMFIAKLLSGGHLKYESDFKINIPNVKFESNSDDKIIDFQSLKNPSICN
TQTDVGKDKITEVSGHVQSLTLDCSDSDDEDENDEREIVKRIVFLKDLMKEYEKTGES
RKAPLIPLLKQTVKLIRQKADFQLEVGYYAQGILSSIVCLNNEFDEPLFEQWRINALT
SILVVLPEKVSGAINILFNSELSLQQRMSLLSALGLSARELRGLDDPTIVKPKFDFPT
NRLPWDDQSHHNSRLVEVQESTSMIKKTKTVWKSRKLGKDREKGTQNRFRKYAGLFFY
PLAHGWLNGIDVGTYNQLFKSHYLTTLRIIYSCANPVHDFESMTELMNHIISSAIEEG
ISLNKG"
gene 666744..669596
/gene="Mdr1"
/locus_tag="P301_G12346"
mRNA 666744..669596
/gene="Mdr1"
/locus_tag="P301_G12346"
/product="Mdr1p"
CDS 666744..669596
/gene="Mdr1"
/locus_tag="P301_G12346"
/note="Similar to YGR100W 'Cytoplasmic GTPase-activating
protein for Ypt/Rab transport GTPases Ypt6p, Ypt31p and
Sec4p; involved in recycling of internalized proteins and
regulation of Golgi secretory function'"
/codon_start=1
/product="Mdr1p"
/protein_id="EWG91069.1"
/translation="MSFFDSLRQKAPFLDKLADSFTPTLTRDEKFRLKYKLPANENIL
EDTNAEVSFATSIKDGKGHSDRVNNKGRKTAYVYSGRLFLTPHFLVFRDAFDHSSCVL
ILNISTIKRVERSPSESYEFALLVTLYTGAKVLIQFIGIRYRSEQFCDKLKLNLKENI
PNAKTLPAFLETSYSEFLIAKNILGKKDITVPRAGLGQHFKYPGNPTMVKEKAKLRLW
FDYFRENGRNLAVVQTPMFRKLIRIGVPNRMRGEIWELCSGAMYMRYANSGEYERILN
ENAGKTSQAIDEIEKDLKRSLPEYSAYQTEEGIQRLRNVLTAYSWKNPDVGYCQAMNI
VVAGFLIFMSEEQAFWCLCNLCDIYVPGYYSKTMYGTLLDQRVFESFVEDRMPVLWEY
ILQHDIQLSVVSLPWFLSLFFTSMPLEYAVRIMDIFFMNGSITLFQVALAVLKINADD
ILQADDDGMFIAIIKHYFQTLGQSAHPDSSDIKYRQITKFQELLVTAFKEFSVISEEM
AMHARHKYEKGIFQNIETFMKRTQLRHMPKTFNLSSDDLSNIYDMFYQSIETYKISMG
TGSSNMGFEVFIQFLSKFCDSCRPCEKDKDPAFRKQKRNFLQRLFDNWDSAHIGELTL
NDVVTGLDKLVTVDLLQAINYFFSLYDTDGDGELHREEVLQLSEGLLLLTEPWKSGRY
VDLLTKKRIEDDIAENIIKESGGEIATMNQIELPTGVTIDEEKYKVEQAERYLKAASN
FLQRSFEYAKAVDLAEEVNLIDLSDDEGEEKRTVKQKQLESIKANAALDPTHPKVIDL
PTFRMIILADETYELFFSNTLRSSVHVDEHVNIDNKNKVLRSMFDGILADGKRVAEQV
RRRVDSVATRSSIASVESTPTAAASSITTKEEKYDDLDDFTSEHQPENEELLQSSWFE
IDDANETSTKAIQERSFEPLSANSSEEKSNLIEFEA"
gene 669862..670902
/gene="Pcp1"
/locus_tag="P301_G12351"
mRNA 669862..670902
/gene="Pcp1"
/locus_tag="P301_G12351"
/product="Pcp1p"
CDS 669862..670902
/gene="Pcp1"
/locus_tag="P301_G12351"
/note="Similar to YGR101W 'Mitochondrial serine protease
required for the processing of various mitochondrial
proteins and maintenance of mitochondrial DNA and
morphology; belongs to the rhomboid-GlpG superfamily of
intramembrane peptidases'"
/codon_start=1
/product="Pcp1p"
/protein_id="EWG91070.1"
/translation="MSGVSSVMLGLRPATRIFFRSNISVSPSRTFVSYIGRSQSTSIL
KNAPNLEDNVTNLQKIIPKRFFSQTSILKSRWKPIFNEETTNRYVRLNRFQQYQQQRS
GGNPLGSMTILGLSLMAGIYFGSPYLFEHVPPFTYFKTHPKNLVYALLGINVAVFGLW
QLPKCWRFLQKYMLLQKDYVTSKISIIGSAFSHQEFWHLGMNMLALWSFGTSLATMLG
ASNFLSLYMNSAIAGSLFSLWYPKLARLAIVGPSLGASGALFGVLGCFSYLFPHAKIL
LFVFPVPGGAWVAFLASVAWNAAGCALRWGSFDYAAHLGGSMMGVLYGWYISKAVEKQ
RQRRLQAAGRWF"
gene complement(671083..671634)
/locus_tag="P301_G12356"
mRNA complement(671083..671634)
/locus_tag="P301_G12356"
/product="hypothetical protein"
CDS complement(671083..671634)
/locus_tag="P301_G12356"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91071.1"
/translation="MYKTWRLCRTHTVGGLCHDGSHRFVSTGGAKIGKKFENMNQIRD
YLSRPVWSVHEYLGINTKEEKLEPPSAEAVKKLLRLSGLPLEGADIKEIQMRLAKQLS
FINKLHNIPVEGEKHTKEYDARLVQRNTKQLNYTKLLEGISHQKQDAELGEVSGSWKA
TGLAAESKNAYFVVKEGLLKNRK"
gene 671916..673732
/gene="Nop7"
/locus_tag="P301_G12361"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(673943..674866)
/gene="Srb5"
/locus_tag="P301_G12366"
mRNA complement(673943..674866)
/gene="Srb5"
/locus_tag="P301_G12366"
/product="Srb5p"
CDS complement(673943..674866)
/gene="Srb5"
/locus_tag="P301_G12366"
/note="Similar to YGR104C 'Subunit of the RNA polymerase
II mediator complex; associates with core polymerase
subunits to form the RNA polymerase II holoenzyme;
essential for transcriptional regulation; involved in
telomere maintenance'"
/codon_start=1
/product="Srb5p"
/protein_id="EWG91072.1"
/translation="MVQQLSLFGSIGDDGYDLLIPTLTTISGNPPLLYNSLCTVWKPN
PSYDVENVNSRNQLVEPNRIKLSKEVPFSYLIDETMMDKPLNFRILKSFTNDKIPLNY
AMTRNILHNTVPQVTNFNSTNEDQNNSKHTEDTVNESRNSDDIIDVDMDASPAPSNES
CSPWSLQISDIPAAGNNRSVSMQTIAETIILSSAGKNSSVSSLMNGLGYVFEFQYLTI
GVKFFMKHGLILELQKIWQIEEAGNSQITSGGFLLKAYINVSRGTDIDRINYTETVLM
NLKKELQGYIELSVPDRQSMDSRVAHGNILI"
gene 675096..675329
/gene="Vma21"
/locus_tag="P301_G12371"
mRNA 675096..675329
/gene="Vma21"
/locus_tag="P301_G12371"
/product="Vma21p"
CDS 675096..675329
/gene="Vma21"
/locus_tag="P301_G12371"
/note="Similar to YGR105W 'Integral membrane protein that
is required for vacuolar H+-ATPase (V-ATPase) function,
although not an actual component of the V-ATPase complex;
functions in the assembly of the V-ATPase; localized to
the yeast endoplasmic reticulum (ER)'"
/codon_start=1
/product="Vma21p"
/protein_id="EWG91073.1"
/translation="MAVDVPRAVINKLMLFTAAMVVLPVLTFFIIQQFTPNTLISGGL
AAAMANVVLIVYIVVAFREDTEDHKVDGNKKED"
gene complement(675486..676283)
/locus_tag="P301_G12376"
mRNA complement(675486..676283)
/locus_tag="P301_G12376"
/product="hypothetical protein"
CDS complement(675486..676283)
/locus_tag="P301_G12376"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91074.1"
/translation="MVFGQLYALFIFTLSCCISKTVQADSSKESSSFISFDKESNWDT
ISTISSTADVISSVDSAIAVFEFDNFSLLDNLMIDEEYPFFNRFFANDVSLTVHDDSP
LNISQSLSPIMEQFTVDELPESASDLLYEYSLDDKSIVLFKFTSDAYDLKKLDEFIDS
CLSFLEDKSGDNLTVVINSLGWAFEDEDGDDEYATEETLSHHDNNKGKEGDDDILSSI
WTEGLLMCLIVSALLLFILIVALSWISNLDITYGALEKSTNPIKKNN"
gene 677157..677238
/locus_tag="P301_G12381"
tRNA 677157..677238
/locus_tag="P301_G12381"
/product="tRNA-Leu"
/note="TL(GAG)G"
gene complement(677434..677529)
/locus_tag="P301_G12386"
tRNA complement(677434..677529)
/locus_tag="P301_G12386"
/product="tRNA-Lys"
/note="TK(UUU)G2"
gene 680115..681529
/gene="Clb1"
/locus_tag="P301_G12406"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(681836..682978)
/gene="Clb6"
/locus_tag="P301_G12411"
mRNA complement(681836..682978)
/gene="Clb6"
/locus_tag="P301_G12411"
/product="Clb6p"
CDS complement(681836..682978)
/gene="Clb6"
/locus_tag="P301_G12411"
/note="Similar to YGR109C 'B-type cyclin involved in DNA
replication during S phase; activates Cdc28p to promote
initiation of DNA synthesis; functions in formation of
mitotic spindles along with Clb3p and Clb4p; most abundant
during late G1'"
/codon_start=1
/product="Clb6p"
/protein_id="EWG91075.1"
/translation="MNCIPSPISERKIQINNEDCIGKENAFHTIPRESSINLTPHSTN
EKKVLSEVNSNKIDSLQLPRGKLQRDSTHLEKTRKRQLSNDSTDPIEPKTVKKIKCHQ
WKNLDSIEMDDPFMVAEYTDSIFSHLYEKEIQMLPTHNYLMDTQSPYHLKSSMRALLI
DWLVEVHEKFHCLPETLFLAINLLDRFLSQNVVKLNKLQLLCITCLFIACKFEEVKLP
KITNFAYVTDGAATVEGIRKAELFVLSSLGYNISLPNPLNFIRRISKADNYCIETRNM
AKFIMEYSICCNKFIHLKPSYLAAMSMYIARKIKNENSKWDETFIHYSGGIDIESDPA
FKDFISELVEDIAVPDTNLDSLRLKYKKPKHGMVYFKVFDWCKQKR"
gene complement(683582..683653)
/locus_tag="P301_G12421"
tRNA complement(683582..683653)
/locus_tag="P301_G12421"
/product="tRNA-Cys"
/note="TC(GCA)G"
assembly_gap 683826..684183
/estimated_length=358
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 684829..686166
/locus_tag="P301_G12426"
mRNA 684829..686166
/locus_tag="P301_G12426"
/product="hypothetical protein"
CDS 684829..686166
/locus_tag="P301_G12426"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91076.1"
/translation="MFKSTLNSIIRRPLKGFQLLRGADSSNTRPQSPRASARDVTEKQ
ILRTPSAPTAIPLREIIYRVPSLFPRPLEDSVKDFRDFIKNEDAFQTELLKTLPFYPT
PSESKTARLIRTVVDDEGNYINEFCIRPRKTSVPEADLKHLVFIHGYGAGLGFFIKNF
EDIPLLDNEWCIHAIDLPGYGFSSRPKFPFEYPRDNIHSVQDWFHERIHTWFSKRNLL
NRPEKNIVMAHSLGSYLMALYLQKYKESPSFKKLILCSPAGVSYRDFNNTASEVEKWK
PPPWWYVKLWDRNISPFTLVRNFRQLGSKITSGWSYRRFKHILNGDPEQSKRFEALHR
YAYAIFNKRGSGEYLLSFALKCGGEPRLSLEQQLFDGKKSDILKNSNCDWLWLYGDDD
WMDVNGGLRVSRFLKEKLKQKSNVIIVPHSGHHLYLDNYKFFNNILTKEMQKI"
gene 686948..688150
/locus_tag="P301_G12436"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 688478..689647
/gene="Shy1"
/locus_tag="P301_G12441"
mRNA 688478..689647
/gene="Shy1"
/locus_tag="P301_G12441"
/product="Shy1p"
CDS 688478..689647
/gene="Shy1"
/locus_tag="P301_G12441"
/note="Similar to YGR112W 'Mitochondrial inner membrane
protein required for assembly of cytochrome c oxidase
(complex IV); associates with complex IV assembly
intermediates and complex III/complex IV supercomplexes;
similar to human SURF1 involved in Leigh Syndrome'"
/codon_start=1
/product="Shy1p"
/protein_id="EWG91077.1"
/translation="MSLLGARSTYRWFSIAASIPTKNAIGKSTYLLASRNQQYRGIIT
STVDWKPIKTGKSPNDDSRRERSFGKKIVLGLMFAMPIISFYLGTWQVRRLKWKTKLI
AACETKLTYEPIPLPKSFTPDMCEDWEYRKVILTGHFLHNEEMFVGPRKKNGEKGYFL
FTPFIRDDTGEKVLIERGWISEEKVAPDSRNLHHLSLPQEEHLKVVCLVRPPKKRGSL
QWAKKDPNSRLWQVPDIYDMARSSGCTPIQFQALYDMKDHPIIEEHTRNEASQNNSTS
SLWKFWKREPTTAVNGTQAVDNNTSKPRSRQEMPTDQTIEFDERQFIKAGVPIGRKPT
IDLKNNHLQYLVTWYGLSFLSTIFLIVALRKAKRGGVVSQDQLMKEKLKHSRKYM"
gene 690013..691043
/gene="Dam1"
/locus_tag="P301_G12456"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 691528..695882
/gene="Spt6"
/locus_tag="P301_G12471"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(696167..697597)
/locus_tag="P301_G12476"
mRNA complement(696167..697597)
/locus_tag="P301_G12476"
/product="hypothetical protein"
CDS complement(696167..697597)
/locus_tag="P301_G12476"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91078.1"
/translation="MADNSTSRFYTNLLIANYLKHNGLEDTLAAFIRETALPLSALEK
SDSSNSNVGEIPLEDLQSVVEDRIYYKRRSFKDRFKTLSINDDLAPIDNAKYGIQPWN
HSLKFSIDVKLNKSLPKDTLFISATFTEDSKYILLSSATGYLVIYDIEKATSKSFKIN
EKVKSIVKLYGPIGSSGYQYVCPMNGSFYLLNNDFELVNNAVWKIHARMITHIKICNV
TESSWFVITSGMDNFLRLSLLEIKNGNTFLTKLSEIKLASNCTSLNVIANGDGNGQNS
FSVFLTRAEYTHIACYSIIDAKNLVHSYNIALNNAEFSTYAFNIRDVMAVDYVHSNTK
DTIGLSPSTMLVVATSHKPYMRLILVEIPMNTGHPKAMKLDKVQTYYDKILRNFATEI
YQDDFSLPILGKLESSNGVLVGNDEGIYSVDLMTGDSRILNIPGEANSLHDRIKCMDI
SKDQMRMVAGTSTKSIYILNVIRNAQ"
gene 698090..698843
/gene="Rps23a"
/locus_tag="P301_G12481"
mRNA join(698090..698154,698471..698843)
/gene="Rps23a"
/locus_tag="P301_G12481"
/product="Rps23ap"
CDS join(698090..698154,698471..698843)
/gene="Rps23a"
/locus_tag="P301_G12481"
/note="Similar to YGR118W 'Ribosomal protein 28 (rp28) of
the small (40S) ribosomal subunit, required for
translational accuracy; nearly identical to Rps23Bp and
similar to E. coli S12 and rat S23 ribosomal proteins;
deletion of both RPS23A and RPS23B is lethal'"
/codon_start=1
/product="Rps23ap"
/protein_id="EWG91079.1"
/translation="MGKGKPRGLNSARKLRVHRRNNRWAENNYKKRLLGTAFKSSPFG
GSSHAKGIVLEKLGIESKQPNSAIRKCVRVQLIKNGKKVTAFVPNDGCLNFVDENDEV
LLAGFGRKGKAKGDIPGVRFKVVKVSGVSLLALWKEKKEKPRS"
gene complement(699146..700786)
/gene="Nup57"
/locus_tag="P301_G12486"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 700320..700411
/estimated_length=92
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(701148..701936)
/gene="Cog2"
/locus_tag="P301_G12496"
mRNA complement(701148..701936)
/gene="Cog2"
/locus_tag="P301_G12496"
/product="Cog2p"
CDS complement(701148..701936)
/gene="Cog2"
/locus_tag="P301_G12496"
/note="Similar to YGR120C Essential component of the
conserved oligomeric Golgi complex (Cog1p through Cog8p),
a cytosolic tethering complex that functions in protein
trafficking to mediate fusion of transport vesicles to
Golgi compartments"
/codon_start=1
/product="Cog2p"
/protein_id="EWG91080.1"
/translation="MDFLNDDELDLDLPVTAEISKELFATEIEKYRESETNGTDVDNF
DVDRFLVQKNFHYLPLDSLIRDLSGLSQRMVQTLLEQIRSNYDDYLTFSNTYTDEENE
TLINLEKTQSDLQKFMTQLDHLIKDDISNTQEIIKDVLEYLKKLDEIYGSLRNHSQLT
EALSLGKRLSKSLHEMCGIEPLEEEICSGLIEQLYKLITASRRILESCADSNSPYIHH
LRNDYQDLLQEFQISLKILTEKCLENPSSLQNLSLTLVSIIKTA"
gene 702252..702325
/locus_tag="P301_G12501"
tRNA 702252..702325
/locus_tag="P301_G12501"
/product="tRNA-Asn"
/note="TN(GUU)G"
gene complement(702564..704042)
/gene="Mep1"
/locus_tag="P301_G12506"
mRNA complement(702564..704042)
/gene="Mep1"
/locus_tag="P301_G12506"
/product="Mep1p"
CDS complement(702564..704042)
/gene="Mep1"
/locus_tag="P301_G12506"
/note="Similar to YGR121C 'Ammonium permease; belongs to a
ubiquitous family of cytoplasmic membrane proteins that
transport only ammonium (NH4+); expression is under the
nitrogen catabolite repression regulation'"
/codon_start=1
/product="Mep1p"
/protein_id="EWG91081.1"
/translation="MESRTTGPLTTETYDGPTVAFMILGAALVFFMVPGLGFLYSGLA
RRKSALALIWVVLMATLVGILQWYFWGYSLAFSKSAPNNKFIGNLDSFGFRNVYGKKF
DEDAYPELAYATFQMMFSCVNLSIIAGATAERGRLLPHMVFLFILATIGYCPVTYWIW
SPGGWAYQWGVLDWAGGGNIEILSAVSGFVYSWFLGKRNEKLLINFRPHNVSLVTLGT
SILWFGWLLFNSASSLSPNLRSVYAFMNTCLSAITGGMTWCLLDYRSEKKWSTVGLCS
GIISGLVAATPSSGCITLYGSLIQGIVAGVVCNFATKLKYYAKVDDAMDILAEHGVAG
VIGLIFNALFGADWVIGMDGTTEHEGGWVTHNYKQMYKQIAYIAASIGYTAAVTAIIC
FVLGYIPGMRLRISEEAEEAGMDEDQIGEFAYDYVEVRRDYYLWGVDEDSQRSDVNHR
VNNAHLAAERSSSGTNSSSDGNGEMIQSEKILPIHQEDPANR"
assembly_gap 704091..704256
/estimated_length=166
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 704527..704742
/locus_tag="P301_G12516"
mRNA 704527..704742
/locus_tag="P301_G12516"
/product="hypothetical protein"
CDS 704527..704742
/locus_tag="P301_G12516"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91082.1"
/translation="MSRHKYKLWMCIAKGKRGVGERNTFVPKCSYAPFCASEVLDQLL
RGDNSIVYNFYLKNGSGSVYIGSVFLI"
gene 705050..706258
/locus_tag="P301_G12521"
mRNA 705050..706258
/locus_tag="P301_G12521"
/product="hypothetical protein"
CDS 705050..706258
/locus_tag="P301_G12521"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91083.1"
/translation="MRENAMSTKKLPYKLSGSSKITTSIPNDLIILRNNCINSLNSSS
SKADSITCIDTWLKYTEGLLTHRYEANNDAALIEEEIAIALINVAVFYQDIGIETLYR
AYESSQASNNLWTTSGTYLKRGLGLICFLGKNFQINTANDCQKMQVLNVLNQLSLEFQ
LLQQLGIVVLALSKLRSKISKDAVADLEPQELEELGKSSVFYAKLCIGSYSTASQCQG
GRIVDALFMNYLQSLTYLFLSINQYNIDECGIAIGMLQESIKKLLNIVPNSQLKELDI
LSSTDITKKRDLIKMSFKRKIHGSTLKNQRIFEKKVPFSSKAYMMPLLKSSLDDFVIP
LTILLRYRYQTTNENYSFKTVETDVSKLKELFPRGKSSDIEGTVWSFQDGHLTFADSN
NATHNCGNYF"
assembly_gap 706304..707687
/estimated_length=1384
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(707783..709324)
/gene="Ppt1"
/locus_tag="P301_G12526"
mRNA complement(707783..709324)
/gene="Ppt1"
/locus_tag="P301_G12526"
/product="Ppt1p"
CDS complement(707783..709324)
/gene="Ppt1"
/locus_tag="P301_G12526"
/note="Similar to YGR123C 'Protein serine/threonine
phosphatase with similarity to human phosphatase PP5;
present in both the nucleus and cytoplasm; expressed
during logarithmic growth; computational analyses suggest
roles in phosphate metabolism and rRNA processing'"
/codon_start=1
/product="Ppt1p"
/protein_id="EWG91084.1"
/translation="MSTPTAADRAKALERKNEGNVFVKEKHFLKAIEKYTEAIDLDST
QSIYFSNRAFAHFKVDNFQSALNDCDEAIKLDPKNIKAYHRRALSCMALLEFKKARKD
LNVLLKAKPNDPAATKALLTCDRFIREERFRKAIGGAENEAKISLCQTLNLSSFDANA
DLANYEGPKLEFEQLYDDKNAFKGAKIKNMSQEFISKMVNDLFLKGKYLPKKYVAAII
SHADTLFRQEPSMVELENNSTPDVKISVCGDTHGQFYDVLNLFRKFGKVGPKHTYLFN
GDFVDRGSWSCEVALLFYCLKILHPNNFFLNRGNHESDNMNKIYGFEDECKYKYSQRI
FNMFSQSFESLPLATLINNDYLVMHGGLPSDPSATLSDFKNIDRFAQPPRDGAFMELL
WADPQEANGMGPSQRGLGHAFGPDITDRFLRNNKLRKIFRSHELRMGGVQFEQKGKLM
TVFSAPNYCDSQGNLGGVIHVVPGHGILQAGRNDDQNLIIETFEAVEHPDIKPMAYSN
GGFGL"
gene 710242..710315
/locus_tag="P301_G12536"
tRNA 710242..710315
/locus_tag="P301_G12536"
/product="tRNA-Ile"
/note="TI(AAU)G"
gene 711060..>712777
/gene="Asn2"
/locus_tag="P301_G12541"
mRNA 711060..>712777
/gene="Asn2"
/locus_tag="P301_G12541"
/product="Asn2p"
CDS 711060..>712777
/gene="Asn2"
/locus_tag="P301_G12541"
/note="Similar to YGR124W 'Asparagine synthetase, isozyme
of Asn1p; catalyzes the synthesis of L-asparagine from
L-aspartate in the asparagine biosynthetic pathway'"
/codon_start=1
/product="Asn2p"
/protein_id="EWG91085.1"
/translation="MCGIFAAFKHEDIHNFKPKALQLSKKIRHRGPDWSGNAVMNSTI
FVHERLAIVGLDSGAQPITSADGEYMLGVNGEIYNHIQLREMCSDYKFQTFSDCEPII
PLYLEHDIDAPKYLDGMFAFCLYDSKKDRIVAARDPIGVVTLYMGRSSQSPETVYFAS
ELKCLTDVCDSIISFPPGHVYDSETDKITRYFTPDWLDEKRIPSTPVDYHAIRHSLEK
AVRKRLMAEVPYGVLLSGGLDSSLIAAIAARETEKANADANEDNNVDEKQLAGIDDQG
HLHTSGWSRLHSFAIGLPNAPDLQAARKVAKFIGSIHHEHTFTLQEGLDALDDVIYHL
ETYDVTTIRASTPMFLLSRKIKAQGVKMVLSGEGSDEIFGGYLYFAQAPSAAEFHTES
VQRVKNLHLADCLRANKSTMAWGLEARVPFLDKDFLQLCMNIDPNEKMIKPKEGRIEK
YILRKAFDTTDEPDVKPYLPEEILWRQKEQFSDGVGYSWIDGLRDTAERAISDAMFAN
PKADWGDDIPTTKEAYWYRLKFDAWFPQKTAADTVMRWIPKADWGCAEDPSGRYAKIH
EKHVSA"
assembly_gap 712778..713296
/estimated_length=519
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 713441..716552
/locus_tag="P301_G12546"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 715557..715653
/estimated_length=97
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 716952..717644
/locus_tag="P301_G12551"
mRNA 716952..717644
/locus_tag="P301_G12551"
/product="hypothetical protein"
CDS 716952..717644
/locus_tag="P301_G12551"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91086.1"
/translation="MPVPSVTVTTDNEYEDISSFSSIDSYKPEPFTGFKDSEAPEQPL
LKNDTIVGKGQLEDDSNVDDQHRHSDVHSHHSSSTLKRPTSNSIEKMVTHNALEGNSE
TVDSLKEDGLNLNKKALPDITAPVTNSAHDAAFPEEYRLETETGLVKLKTLESLKRED
SRVSSTKKEHINDHTDMHSTRSKVTTNSQGSSLEPNKLNMAVEKNKKRIEKYQKHKSE
KGIKGFFHRIFD"
gene 717913..718827
/locus_tag="P301_G12556"
mRNA 717913..718827
/locus_tag="P301_G12556"
/product="hypothetical protein"
CDS 717913..718827
/locus_tag="P301_G12556"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91087.1"
/translation="MCILMATRAHPDYELILISNRDEFLARKTHATCWHNNDFILSPY
DLAKTSAEKQIFGTWSGINKEGKLATILNLKLDNEQNNTKSRSRGLLPFIFLSIHKAD
FEDWDNYKKFEGHYDGLKSTGDFNFFYGDVIKKQYKVIDSLGRTFDVLSSTCRKDLDS
YMVVSNGKFYDSSSIPGQAWEKVKVARDSLENLVLENIESDEEKIISSCFQLASKSSL
PSTISNPDVLQMVDPNVTMNTIYVPPLRRPPRDDLGASIPDGDYYGTRSQIVXXXXXX
XKRIKTYSQRHIVGAALFIPVPPSVIIT"
assembly_gap 718723..718742
/estimated_length=20
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(719574..720305)
/locus_tag="P301_G12561"
mRNA complement(719574..720305)
/locus_tag="P301_G12561"
/product="hypothetical protein"
CDS complement(719574..720305)
/locus_tag="P301_G12561"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91088.1"
/translation="MENCTFFFNGTDCQEAFRKTILEPAVLRRQNEDPRFSSIEILVP
YTEDAVQVYFTKVNAQWRLIHGKKECKLTMLENVKLPKETYRCKLAWSCQRIRISFSP
LDFLPDMSDFFICANLSPACLVSYWLIDIFFRMIDDFQNIRPKSMKVDDKMQYLSDII
NEQGASPEKWDTIARKTNTRLFEKRVWKNEEFFFDGTDCQAFFERNFSSLLFSKKSAS
PRSLNVELWKYIQEAQLSCNYEPLP"
assembly_gap 721366..721439
/estimated_length=74
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(721600..723381)
/gene="Utp8"
/locus_tag="P301_G12566"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 724049..724696
/gene="Syf2"
/locus_tag="P301_G12571"
mRNA 724049..724696
/gene="Syf2"
/locus_tag="P301_G12571"
/product="Syf2p"
CDS 724049..724696
/gene="Syf2"
/locus_tag="P301_G12571"
/note="Similar to YGR129W 'Member of the NineTeen Complex
(NTC) that contains Prp19p and stabilizes U6 snRNA in
catalytic forms of the spliceosome containing U2, U5, and
U6 snRNAs; isy1 syf2 cells have defective spindles
activiating cell cycle arrest'"
/codon_start=1
/product="Syf2p"
/protein_id="EWG91089.1"
/translation="MDFYKLDEKLKELKRKRVDVSIKSRKLADREIQEVSANRKPRVY
SMEDVNDADESVGDTESPEKEKAFHYTVQEYDAWERRHPQGKTGQSQRGGISYDQLAK
LSYEKTLRNLATQTQNSSKQDSSADEEDNKNVPKKGRIGKVQKDTKTGKITIADDDKL
VNKLAVSLQSESKKRYEARKRQMQNAKTLYGVESFINDKNKQFNEKLSRESKGSE"
gene complement(725043..727493)
/locus_tag="P301_G12576"
mRNA complement(725043..727493)
/locus_tag="P301_G12576"
/product="hypothetical protein"
CDS complement(725043..727493)
/locus_tag="P301_G12576"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91090.1"
/translation="MLFNINRQEDDPFTQLINQSSANTQNQQAHQQESPYQFLQKVVS
NEPKGKEEWVSPFRQDALANRQNNRAYGEDAKNRKFPTVSATSAYSKQQPKDLGYKNI
PKNAKRAKDIRFPTYLTQNEERQYQLLTELELKEKHLKYLKKCQKITDLTKDEKDDTD
TTTSSSTSTSSSSSSSSSSSSSSSSDEGDVTSTTTSEATEATADTATTTTTTTSTSTT
STSTTNAVENSADEATSVEEEHEDKVSESTSIGKGTADSAQINVAEPISSENGVLEPR
TTDQSGGSKSGVVPTDEQKEEKSDVKKVNPPSGEEKKEVEAEGDAEEETEQSSAEESA
ERTSTPETSEPESEEDESPIDPSKAPKVPFQEPSRKERTGIFALWKSPTSSSTQKSKT
AAPSNPVATPENPELIVKTKEHGYLSKAVYDKINYDEKIHQAWLADLRAKEKDKYDAK
NKEYKEKLQDLQNQIDEIENSMKAMREETSEKIEVSKNRLVKKIIDVNAEHNNKKLMI
LKDTENMKNQKLQEKNEVLDKQTNVKSEIDDLNNEKTNVQKEFNDWTTNLSNLSQQLD
AQIFKINQINLKQGKVQNEIDNLEKKKEDLVTQTEENKKLHEKNVQVLESVENKEYLP
QINDIDNQISSLLNEVTIIKQENANEKTQLSAITKRLEDERRAHEEQLKLEAEERKRK
EENLLEKQRQELEEQAHQAQLDHEQQITQVKQTYNDQLTELQDKLATEEKELEAVKRE
RTRLQAEKAIEEQTRQKNADEALKQEILSRQHKQAEGIHAAENHKIPNDRSQKNTSVL
PKDDSLYEYHTEEDVMYA"
gene 728375..728899
/locus_tag="P301_G12586"
mRNA 728375..728899
/locus_tag="P301_G12586"
/product="hypothetical protein"
CDS 728375..728899
/locus_tag="P301_G12586"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91091.1"
/translation="MLSAADNLVRIINAVFLIISIGLISGLIGTQTKHSSRVNFCMFA
AVYGLVTDSLYGFLANFWTSLTYPAILLVLDFLNFIFTFVAATALAVGIRCHSCKNKT
YLEQNKIIQGSSSRCHQSQAAVAFFYFSCFLFLIKVTVATMGMMQNGGFGSNTGFSRR
RARRQMGIPTISQV"
gene complement(729238..730101)
/gene="Phb1"
/locus_tag="P301_G12591"
mRNA complement(729238..730101)
/gene="Phb1"
/locus_tag="P301_G12591"
/product="Phb1p"
CDS complement(729238..730101)
/gene="Phb1"
/locus_tag="P301_G12591"
/note="Similar to YGR132C 'Subunit of the prohibitin
complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner
mitochondrial membrane chaperone that stabilizes newly
synthesized proteins; determinant of replicative life
span; involved in mitochondrial segregation'"
/codon_start=1
/product="Phb1p"
/protein_id="EWG91092.1"
/translation="MSNSAKLIDVITKVALPIGIIASGIQYSMYDVKGGSRGVIFDRI
NGVKQQVVGEGTHFLVPWLQKAIIYDVRTKPKSIATNTGTKDLQMVSLTLRVLHRPEV
LQLPAIYQNLGLDYDERVLPSIGNEVLKSIVAQFDAAELITQREIISQKIRKELSTRA
NEFGIKLEDVSITHMTFGPEFTKAVEQKQIAQQDAERAKFLVEKAEQERQASVIRAEG
EAESAEFISKALAKVGDGLLLIRRLEASKDIAQTLANSSNVVYLPSQHSGGGNSESSG
SPNSLLLNIGR"
assembly_gap 730298..730494
/estimated_length=197
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 730545..731096
/gene="Pex4"
/locus_tag="P301_G12596"
mRNA 730545..731096
/gene="Pex4"
/locus_tag="P301_G12596"
/product="Pex4p"
CDS 730545..731096
/gene="Pex4"
/locus_tag="P301_G12596"
/note="Similar to YGR133W Peroxisomal ubiquitin
conjugating enzyme required for peroxisomal matrix protein
import and peroxisome biogenesis"
/codon_start=1
/product="Pex4p"
/protein_id="EWG91093.1"
/translation="MPNFWILENRRSYTSDTCMSRIVKEYKVILKTLASDDPIANPYR
GIIESLNPIDETDLSKWEAIISGPSDTPYENHQFRILIEVPSSYPMNPPKISFMQNNI
LHCNVKSATGEICLNILKPEEWTPVWDLLHCVHAVWRLLREPVCDSPLDVDIGNIIRC
GDMSAYQGIVKYFLAERERINNH"
gene 731419..734787
/gene="Caf130"
/locus_tag="P301_G12601"
mRNA 731419..734787
/gene="Caf130"
/locus_tag="P301_G12601"
/product="Caf130p"
CDS 731419..734787
/gene="Caf130"
/locus_tag="P301_G12601"
/note="Similar to YGR134W Part of the
evolutionarily-conserved CCR4-NOT transcriptional
regulatory complex involved in controlling mRNA
initiation, elongation, and degradation"
/codon_start=1
/product="Caf130p"
/protein_id="EWG91094.1"
/translation="MTKKKAATNYAERQNLASEDSSGDSVHFKDFIPLQELLKDKNYV
PSVENLEKILYNETMFNDQKICSNLLLEALIITLFTTISGKSALRLIQTSSLKERKSW
AQSFENNSSSYASIVLSWKDNDILLLKFLRFLLANKTAPLQINRYNLPEYKLPLSFLI
VSKITIPSILLNETYNLLKDYLYSITGRIESLISCSSTFDKPALVVRKILKDYNRMIE
CRNFYFWYSFNAENRVNLTFSDNISLLMENDEGNAGSGLDDSRFDHQKQPREAIMGRT
INDQEQIYSFELNQDGTLEIPNVMEHSLLRHELLFKILNLTTVLTPLLELQFSTLCGL
VDPLMQPTPNDKHIISIDFLFQLFLGLMSQSIKTSQEHNDHYDWKFYMCFNMQKIIDA
TMLRLNCFDFDILNSVNNTDNAVHWKTQLHRWLPHGLNTQDLELLYMIDILAVYTIYK
LYEKIPIQLNPFLFSLISLWKNLSCVILLALEIDRIEEENGTYETPLMVRATIRGAAA
LRSVIATVLNGLVKNNDHDFKHESLNTFMSPYGRKLCHGALYADLRSHTASLLALGAS
IEDVTDLFADLQSGDRFDEDIRYMFDYECEDYDESFSESDHGGLDESVVNPTEKIASG
SNNVFFRRRCNCIFNDDKLVAEDGANEAFGSTNSENVEGAMHNNRNAVHNATTATSDH
VVTSPNPLSVRSRSTFEFDYSGEDWRDVPRDFNMYYSPSYSFIHEPKLDVIFSLTLRG
ATEKLNKEESILLVRSVASCVRNEQDQMILADLESNFSASINGDVEGEGNTKMSKIDN
EDLRRTTPDDIYEIWSEESAFERMLNVNHDVAWRLMDEMLMCTGYRRILIWFLTHLEL
KHSLIYYVFELIMGLRGKPFSGEASDQDKKDDMIYEILKKKQKNEDASGLPFSRQGPI
VLSDIETKMLLQEFFMNAAIFLSSKNNEEENEDGEKISLYSLGLVRLICYMVQTLIAN
DKFFFTKSECTFELQTLLMTWIGILPEAKDLFFKIKTRLAMEEEDSADTMQHEGRKNS
DIEKKLNAKPASELNLKLLNLFPSKPANKDDSSPINTLRSFIADYSFDTQVNPPGRRV
VFYDGKILPLPKADKPIPLHEYITLAELDVGDSE"
gene 735041..735817
/gene="Pre9"
/locus_tag="P301_G12606"
mRNA 735041..735817
/gene="Pre9"
/locus_tag="P301_G12606"
/product="Pre9p"
CDS 735041..735817
/gene="Pre9"
/locus_tag="P301_G12606"
/note="Similar to YGR135W 'Alpha 3 subunit of the 20S
proteasome, the only nonessential 20S subunit; may be
replaced by the alpha 4 subunit (Pre6p) under stress
conditions to create a more active proteasomal isoform'"
/codon_start=1
/product="Pre9p"
/protein_id="EWG91095.1"
/translation="MGSRRYDSRTTIFSPEGRLYQVEYALESISHAGTAIGIMASDGI
VLAAERKVTSTLLEQDTSTEKLYKLNDKIAVAVAGLTADAEILINTARIHAQNYLKTY
NEDIPVEILVRRLSDIKQGYTQHGGLRPFGVSFIYAGYDDRYGYQLYTSNPSGNYTGW
KAISVGANTSAAQTLLQMDYKDDMKVDDAIELALKTLSKTTDSSALTYDRLEFATIRK
GANDGEVYQKIFKPQEIKDILVKTGITKKDEDEEADEDMK"
gene 736077..736802
/gene="Lsb1"
/locus_tag="P301_G12611"
mRNA 736077..736802
/gene="Lsb1"
/locus_tag="P301_G12611"
/product="Lsb1p"
CDS 736077..736802
/gene="Lsb1"
/locus_tag="P301_G12611"
/note="Similar to YGR136W 'Protein containing an
N-terminal SH3 domain; binds Las17p, which is a homolog of
human Wiskott-Aldrich Syndrome protein involved in actin
patch assembly and actin polymerization'"
/codon_start=1
/product="Lsb1p"
/protein_id="EWG91096.1"
/translation="MSASLVNRSLKNIRNELEFLKESNVISGDIFELINSKLPEKWDG
NQRSPQNADTEEYVEALYDFEAQQDGDLSLKTGDKIQVLEKISPDWYRGKSNNKIGIF
PANYVKPAFTRSASPKSAEAASSSTVSRPSVPPPSYEPAASQYPSQQVSAPYAPPAGY
MQAPPPQQQQAPLPYPPPFTNYYQQPQQQYAPPSQQAPVEAQPQQSSGASSAFKSFGS
KLGNAAIFGAGSAIGSDIVNSIF"
gene complement(737411..739255)
/gene="Tpo2"
/locus_tag="P301_G12621"
mRNA complement(737411..739255)
/gene="Tpo2"
/locus_tag="P301_G12621"
/product="Tpo2p"
CDS complement(737411..739255)
/gene="Tpo2"
/locus_tag="P301_G12621"
/note="Similar to YGR138C 'Polyamine transport protein
specific for spermine; localizes to the plasma membrane;
transcription of TPO2 is regulated by Haa1p; member of the
major facilitator superfamily'"
/codon_start=1
/product="Tpo2p"
/protein_id="EWG91097.1"
/translation="MSDQESVVSFNSQNTSMVDVEGQQPQQYVPSKTNSRANQLKLTK
TETVKSLQDLGVTSAAPVPDINAPQTAKNNIFPEEYTMETPSGLVPVATLQSMGRTAS
ALSRTRTKQLNRTATNSSSTGKEEMEEEETEEREDQSGENELDPEIEFVTFVTGDPEN
PHNWPSWVRWSYTVLLSILVICVAYGSACISGGLGTVEKKYHVGMEAAILSCSLMVIG
FSLGPLIWSPVSDLYGRRVAYFVSMGLYVIFNIPCALAPNLGCLLACRFLCGVWSSSG
LCLVGGSIADMFPSETRGKAIAFFAFAPYVGPVVGPLVNGFISVSTGRMDLIFWVNMA
FAGVMWIISSAIPETYAPVILKRKAARLRKETGNPKIMTEQEAQGVSMSEMMRACLLR
PLYFAVTEPVLVATCFYVCLIYSLLYAFFFAFPVIFGELYGYKDNLVGLMFIPIVIGA
LWALATTFYCENKYLQIVKQRKPTPEDRLLGAKIGAPFAAIALWILGATAYKHIIWVG
PASAGLAFGFGMVLIYYSLNNYIIDCYVQYASSALATKVFLRSAGGAAFPLFTIQMYH
KLNLHWGSWLLAFISTAMIALPFAFSYWGKGLRHKLSKKDYSIDSVEM"
assembly_gap 739337..739451
/estimated_length=115
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 741076..743946
/gene="Cbf2"
/locus_tag="P301_G12631"
mRNA 741076..743946
/gene="Cbf2"
/locus_tag="P301_G12631"
/product="Cbf2p"
CDS 741076..743946
/gene="Cbf2"
/locus_tag="P301_G12631"
/note="Similar to YGR140W 'Essential kinetochore protein,
component of the CBF3 multisubunit complex that binds to
the CDEIII region of the centromere; Cbf2p also binds to
the CDEII region possibly forming a different multimeric
complex, ubiquitinated in vivo'"
/codon_start=1
/product="Cbf2p"
/protein_id="EWG91098.1"
/translation="MRSSILFLLKLMKIMDVQQQQEGMSSEDRFQELVDSLKPRTAHQ
YKTYYTKYIQWCQLNQIIPTPEDNSVNSVPYKDLPISAELIHWFLLDTLITDDKPGEK
REETEDLDEEEENSFKIATLKKIIGSLNFLSKLCKVHENPNANIDTKYLESVTKLHTH
WIDSQKAITTNETNNTNTQVLCPPLLKVSLNLWNPETNHLSEKFFKTCSEKLRFLVDF
QLRSYLNLSFEERSKIRFGSLKLGKRDRDAIIYHKVTHSAEKKDTPGHHQLLALLPQD
CPFICPQTTLAAYLYLRFYGIPSVSKGDGFPNLNADENGSLLQDIPILRGKSLTTYPR
EETFSNYYTTVFRYCHLPYKRREYFNKCNLVYPTWDEDTFRTFFNEENHGNWLEQPEA
FAFPDKIPFDFKKIMNFKSPYTSYSTNAKKDPFPPPKDLLVQIFPEIDEYKRHDYEGL
SQNSRDFLDLMEVLRERFLSNLPWIYKFFPNHDIFQDPIFGNSDFQSYFNDKTIHSKG
SPILSFDILPGFNKIYKNKTNFYSLLIERPSQLTFASSHNPDTHPTQKQESEGPLQMS
QLDTTQLNELLKQQSFEYVQFQTLSNFQILLSVFNKIFEKLEMKKSSRGYILHQLNLF
KITLDERIKKSKIDDADKFIRDNQPIKKEENIVNEDGPNTSRRTKRPKQIRLLSIADS
SDESSTEDSNVFKKDGESIEDGAYGENEDENDSEMQEQLKSMINELINSKISTFLRDQ
MDQFELKINALLDKILEEKVTRIIEQKLGSHTGKFSTLKRPQLYMTEEHNVGFDMEVP
KKLRTSGKYAETVKDNDDHQAMSTTASPSPEQDQEAKSYTDEQEFMLDKSIDSIEGII
LEWFTPNAKYANQCVHSMNKSGNKSWRANCEALYKERKSIVEFYIYLVNHESLDRYKA
VDICEKLRDQNEGSFSRLAKFLRKWRHDHQNSFDGLLVYLSN"
gene 744216..745619
/gene="Vps62"
/locus_tag="P301_G12636"
mRNA 744216..745619
/gene="Vps62"
/locus_tag="P301_G12636"
/product="Vps62p"
CDS 744216..745619
/gene="Vps62"
/locus_tag="P301_G12636"
/note="Similar to YGR141W Vacuolar protein sorting (VPS)
protein required for cytoplasm to vacuole targeting of
proteins"
/codon_start=1
/product="Vps62p"
/protein_id="EWG91099.1"
/translation="MRISKNSHKRQRTRLYFLVTFIIYSIIPCRAVLVPWLDDDPFEA
TLLEMGDEPWSKDILSSTPPLHPSEVTEDNKSLKQRGNVPQYVIDNSPLLHLYSEEKY
WPADVKDFVKRFQLRDHSGEKIINEHLRDLSDLQEYYSVELENGTWGRVSSEGTYMTS
LDDFDKGPDWLLGEQPEYGTGHIKKAPAVLFVVDKGNGWVDAFWFYFYPFNWGPYIMG
SGPWGNHVGDWEHSLVRFYKGEPQYLWMSAHGGGSAYKFEAIEKIKRLRRVDGKLTNE
VIKKPLIFSARGTHAHYASVGQHAHDVPFFFMPLSDFTDRGPLWDPSLNYYAYTVTVG
EKMTPCGAEETKMGLEWLSFKGAWGDKQLRPRDPRQKWCPFQWKYIDGPKGPLFKNME
RVSLCQRFKWWNFWKGCPARRYIKRGEGLDAEKNDLVGDNCGILLYNIRPKWLRSILR
FLTWRGSVCFIMDYFTG"
gene 746099..747243
/gene="Btn2"
/locus_tag="P301_G12641"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 747269..747297
/estimated_length=29
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 747992..748064
/locus_tag="P301_G12646"
tRNA 747992..748064
/locus_tag="P301_G12646"
/product="tRNA-Ala"
/note="tRNA-Ala (Ala1);
TA(AGC)G"
gene 748836..751151
/gene="Skn1"
/locus_tag="P301_G12651"
mRNA 748836..751151
/gene="Skn1"
/locus_tag="P301_G12651"
/product="Skn1p"
CDS 748836..751151
/gene="Skn1"
/locus_tag="P301_G12651"
/note="Similar to YGR143W 'Protein involved in
sphingolipid biosynthesis; type II membrane protein with
similarity to Kre6p'"
/codon_start=1
/product="Skn1p"
/protein_id="EWG91100.1"
/translation="MSVRNLTNNRHSNSENSVSGSENSFYSSNEQSRQSSSLEPADGQ
NVRVSGNPFLGSEEFDEDYNSPSGDDERRGANEYSSSSSINYNNDPNSDTSLLANEKN
SPERNGQRMSDYKGYYAKTNLTSANNLNNHNNNNYKNIISSSNDNSFASHLQPPDRNL
PSHPSSNNMSSFSNNSLIKSPPPFDRYPLVGTRHISAAQSQSQNLINEKKRANMTGSS
SSAHDSSLSSTNLYMGEQDFSPFGGYPASFFPLTLDEKEDDDYIHNPNVEEEAKLDRR
RFVDDFKHMDRRSFLGLLGILFLFMAGIFIFIVLPAITFSGVVYHHEHVHAANSAGSS
SSNTTSKSLTEYQYPQLAAIRTTLVDPDTPDSAKTRVAKDGSKWQLVFSDEFNAEGRT
FYDGDDQFWTAPDIHYDATKDLEWYSPDAVTTTNGTLTLRMDAFRNHDLYYRSGMVQS
WNKLCFTEGALEVSANLPNYGRVTGLWPGMWTMGNLGRPGYLASTQGVWPYSYEACDA
GITPNQSSPDGISYLPGQKLSVCTCDNEDHPNQGVGRGAPEIDILEGEADTILGVGVA
SQSLQIAPFDIWYMPDYDFIEVYNFTTTTMNTYAGGPFQQAVSAISTLNVTWYEFGEE
AGYFQKYAIEYLNDDDNGYIRWFVGENPTFTLYATSLHPSGNIDWRRISKEPMSAILN
LGISNNWAYIDWQYIFFPVTMSIDYVRLYQPKGSTSITCDPEDYPTYDYIQSHLNAYY
NANLTDWEQAGYTFPKNILTGGCSSSKFSLS"
assembly_gap 751463..751561
/estimated_length=99
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 752479..753054
/estimated_length=576
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<753055..754121)
/gene="Thi4"
/locus_tag="P301_G12671"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 754981..755281
/estimated_length=301
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 755413..757536
/gene="Enp2"
/locus_tag="P301_G12676"
mRNA 755413..757536
/gene="Enp2"
/locus_tag="P301_G12676"
/product="Enp2p"
CDS 755413..757536
/gene="Enp2"
/locus_tag="P301_G12676"
/note="Similar to YGR145W 'Essential nucleolar protein,
required for biogenesis of the small ribosomal subunit;
contains WD repeats, interacts with Mpp10p and Bfr2p, and
has homology to Spb1p'"
/codon_start=1
/product="Enp2p"
/protein_id="EWG91101.1"
/translation="MVLKSTSANDVSVYQVSGTNVSRSLPDWIAKKRKRQLKNDLEYQ
NRVELIQDFEFSEASNKIKVSRDGQYCMATGTYKPQIHVYDFANLSLKFDRHTDAENV
DFTILSDDWTKSVHLQNDRSIQFQNKGGLHYTTRIPKFGRSLVYNKVNCDLYVGASGN
ELYRLNLEKGRFLNPFKLDTEGVNHVSINEVNGLLAAGTETNVVEFWDPRSRSRVSKL
YLENNIDNRPFQVTTTSFRNDGLTFACGTSNGYSYIYDLRTSEPSIIKDQGYGFDIKK
IIWLDNVGTENKIVTCDKRIAKIWDRLDGKAYASMEPSVDINDIEHVPGTGMFFTANE
SIPMHTYYIPSLGPSPRWCSFLDSITEELEEKPSDTVYSNYRFITRDDVKKLNLTHLV
GSRVLRAYMHGFFINTELYDKVSLIANPDAYKDEREREIRRRIEKERESRIRSSGAVQ
KPKIKVNKTLVDKLSQKRGDKVAGKVLTDDRFKEMFEDEEFQVDEDDYDFKQLNPVKS
IKETEEGAAKRIRALTAAEESDEERIAMKDGRGHYDYEDEESDEEESDDETNQKSNKE
ELSEKDLRKMEKQKALIERRKKEKEQSERFMNEMKAGTSTSTQRDESAHVTFGEQVGE
LLEVENGKKSNESILRRNQRGEAELTFIPQRKSKKDGNYKSRRHDNSSDEEGIDENGN
KKDNGRSKPRFENRRRASKNAFRGM"
gene complement(757866..758501)
/locus_tag="P301_G12696"
mRNA complement(757866..758501)
/locus_tag="P301_G12696"
/product="hypothetical protein"
CDS complement(757866..758501)
/locus_tag="P301_G12696"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91102.1"
/translation="MSTAFNDYCTVCDRLIPTSPQKTNINTRKIQRDNETKSSLQSNK
LYCSEDCKLKDSNPLNEKLLSHLHKKSKTSHSHNLTPPLSYSKNLTASNLFEPTTSLS
SSPTSSTIPFDELEKLESLLISPLLLPQDGIVNPKQESNPSRVDEYDENEHYLNLADS
LRLDSSYQLHSKAHLGYENNLPRSNDLIDDHLISDQIIENNYNLWFRLSSS"
gene complement(758919..759080)
/locus_tag="P301_G12701"
mRNA complement(758919..759080)
/locus_tag="P301_G12701"
/product="hypothetical protein"
CDS complement(758919..759080)
/locus_tag="P301_G12701"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91103.1"
/translation="MGFLPECNLTCAFLLHSFTFPIAHCPSFSWASFFFTIRPPFFPK
LALVCTIFS"
gene complement(759696..760562)
/gene="Nat2"
/locus_tag="P301_G12706"
mRNA complement(759696..760562)
/gene="Nat2"
/locus_tag="P301_G12706"
/product="Nat2p"
CDS complement(759696..760562)
/gene="Nat2"
/locus_tag="P301_G12706"
/note="Similar to YGR147C Protein with an apparent role in
acetylation of N-terminal methionine residues"
/codon_start=1
/product="Nat2p"
/protein_id="EWG91104.1"
/translation="MMVPRISASPVFKRIFLRWGLVTLPIQKTVSHTLRRDFSAPCRS
MVKCLLLRPGISVHSAQDKKFYSTEEKSSQLDENKSKSNNGKKNEPHGIKGLMAKYGY
SALIVYILLTCVDLPLCFLGVHSLGEEKIKIYLNRGKQLIGMGEPDESKVIQDVRRKQ
AHREAVQAENADKVEDASRKTFNERWQEMKDSTLLAELLIAYGIHKSLIIVRVPLTAV
LTPSFVKLLQRFGIDLMKKQKKVFQTMASGAKIRYKGNNPSDFIKNEGTALDITKRKP
RTKGQKWFDGLM"
assembly_gap 760693..761077
/estimated_length=385
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<761078..761417)
/gene="Rpl24b"
/locus_tag="P301_G12711"
mRNA complement(<761078..761417)
/gene="Rpl24b"
/locus_tag="P301_G12711"
/product="Rpl24bp"
CDS complement(<761078..761417)
/gene="Rpl24b"
/locus_tag="P301_G12711"
/note="Similar to YGR148C 'Ribosomal protein L30 of the
large (60S) ribosomal subunit, nearly identical to Rpl24Ap
and has similarity to rat L24 ribosomal protein; not
essential for translation but may be required for normal
translation rate'"
/codon_start=1
/product="Rpl24bp"
/protein_id="EWG91222.1"
/translation="MKVEVDSFSGAKIYPGRGTLFVRGDSKIFRFQNSKSASLFKQRK
NPRRIAWTVLFRKHHKKGITEEVAKKRSRKTVKAQRPITGASLDLIKERRSLKPEVRK
ANREEKLKANK"
gene 762669..763967
/locus_tag="P301_G12721"
mRNA 762669..763967
/locus_tag="P301_G12721"
/product="hypothetical protein"
CDS 762669..763967
/locus_tag="P301_G12721"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91105.1"
/translation="MYKLDNNDIDDETNNSVSLTSLLEFLDPIASKVVSKYYHGSHPS
KAEQKLRNFEGFRRRKPHHEHDSHHPHHLNRSRSFLQLEDFKVRALQRIRNLDKPLDS
IFFKNSSRLEKAFYPFTLFNIFFIGFLMGRFPEWFHVYYTILFFVLMPIRFYTYYKTK
NHYFLADFCYFVNMLCLLFIWIFPYSYSLFQSCFAFTFGTLCFAVITWRNSLVIHSID
KTTSCFIHIIPPCVMYVIYHGLPLEYKIERFPGAIIQSELDIKKNILWTSLYYLVWQS
LYHYFITLKKSSKIKSGERMTSFEYLTTHQFKNFWAVKLRSPWPMIIYTLSQYFYQLF
TMLLCGIWIRYKLAAALFLTIVFLWASHNGATYYIDHYGKNFEKEVDRLRLEVENLQQ
KLQPDSDAVISDASVNDKDYLNVNRDEDFDDSSSVSSKSD"
gene complement(764099..766696)
/gene="Dmr1"
/locus_tag="P301_G12726"
mRNA complement(764099..766696)
/gene="Dmr1"
/locus_tag="P301_G12726"
/product="Dmr1p"
CDS complement(764099..766696)
/gene="Dmr1"
/locus_tag="P301_G12726"
/note="Similar to YGR150C 'Mitochondrial 15s rRNA-binding
protein; required for intron removal of COB and COX1
pre-mRNAs; contains pentatricopeptide repeat (PPR) motifs;
mutant is respiratory deficient and has defective plasma
membrane electron transport'"
/codon_start=1
/product="Dmr1p"
/protein_id="EWG91106.1"
/translation="MYMARCGPKNNVLCFPFQLSFLFSKRLINKRFKYTLQTEDEKNM
MGSLSKNKIITPEDVEFKLAQLREFSNTLKERIHNTKSVNSDGHQSNSIAPISEDSRN
VNVTKISSVPNEEKSKNLSDLIHSSFLEKMDHLVPKVIRERVADDDILAKNLFDRSHS
NWAPVIDRLYVSEKRFMDIDSREFSVWLNGTVKYLPFHSILHLDEMLLEQINGDVVKF
NTHMYECIFNNLGNLKPTNFNQDGTNDKVILKMKELLERYDKALKITEERINKKEGXX
XXXXXXXXXXXXXXXXXXXXXXXXXXHGLFLLQKFRDDYGITPNKQNLTTVIQFYSRK
EMTKQAWNTFDTMKFLSTKHFPDICTYNTMLRICEKERNFPKALDLFQEIQDHNIKPT
TNTYIMMARVLASSSSNAVVSEGKSDSLRLLGWKYLHELEDKNLYRHKKDDLNLFLAM
MALAAFDGDIELSRALYYLFIAKKYKTLCANWKGNILVDQDTIWKSTLMPEMLNYLML
AYARFDPRNLPVLSGYEKGIELRRKFLREFDSSMRLDDTDKLVKFKLPFLPISDLNSE
AQVLAESNAIWSFNLENGGTRNTLTSSNEAALEDIKKYRQLLDSFAQEAEDFNEFKFK
VMYEVTKMQRESINVNVFNKISLHTYLSIPINLKQQKEFLRRLTFFTFQQHEFEAVIK
RLYEGYRNIPSSHTRDQNSISTEAISVSKPETTEDLNLIMHDIWYITCLRHKIMMDTT
LYELVMKAAIEFQNEDLAKKVWNDRGKFRTTVPFLKMDQRIRIAKDQKFAHLMVEFFT
KQGKYSDAIAIILSSKNRFNWTYSMVRNLHKALEEIEDRNSVEILLDVVNKKSHAKAL
KWEEQELNI"
assembly_gap 765792..765875
/estimated_length=84
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 768202..768274
/locus_tag="P301_G12731"
tRNA 768202..768274
/locus_tag="P301_G12731"
/product="tRNA-Ala"
/note="tRNA-Ala (Ala2);
TA(UGC)G"
gene complement(768460..769278)
/gene="Rsr1"
/locus_tag="P301_G12741"
mRNA complement(768460..769278)
/gene="Rsr1"
/locus_tag="P301_G12741"
/product="Rsr1p"
CDS complement(768460..769278)
/gene="Rsr1"
/locus_tag="P301_G12741"
/note="Similar to YGR152C 'GTP-binding protein of the ras
superfamily required for bud site selection, morphological
changes in response to mating pheromone, and efficient
cell fusion; localized to the plasma membrane;
significantly similar to mammalian Rap GTPases'"
/codon_start=1
/product="Rsr1p"
/protein_id="EWG91107.1"
/translation="MRDYKLVVLGAGGVGKSCLTVQFVQGVYLDTYDPTIEDSYRKTI
EIDNKVFDLEILDTAGIAQFTAMRELYIKSGMGFLLVYSVTDRQSLEELMELREQVLR
IKDSDRVPMVLIGNKADLINERVISVEEGIEVSSKWGRVPFYETSALLRSNVDEVFVD
LVRQIIRNEMESVAVKDARNQSQQFSKIESPSTKLPSSAKQDTKQSNNKQSSKGLYNK
PSQGQAKVKQSTPVNEKHKPSHAVPKSGSSNRTGISATSQQKKKKKNASTCTIL"
gene 769875..770528
/locus_tag="P301_G12746"
mRNA 769875..770528
/locus_tag="P301_G12746"
/product="hypothetical protein"
CDS 769875..770528
/locus_tag="P301_G12746"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91108.1"
/translation="MGKACLNKEVGTYECEGERDTYSFFTSLSDIQDSSSNEEQCGVG
SILSEDSFTFEGSDVSIRLFSLDLNALNENENGSKNPVKFTIPPKIEQRKEARQREKH
LRRVALLPENSRNYLVESSMDSSREYSQPFFDWRHEMVERGEESVKPCGCHKSRKAKC
FKELEMENIEKGDIKKSLFYRDIIEWCRDYEVNKTREVCVPSIHEFYLHGNGSDNLF"
gene complement(770581..771651)
/gene="Gto1"
/locus_tag="P301_G12751"
mRNA complement(770581..771651)
/gene="Gto1"
/locus_tag="P301_G12751"
/product="Gto1p"
CDS complement(770581..771651)
/gene="Gto1"
/locus_tag="P301_G12751"
/note="Similar to YGR154C 'Omega-class glutathione
transferase; induced under oxidative stress; putative
peroxisomal localization'"
/codon_start=1
/product="Gto1p"
/protein_id="EWG91109.1"
/translation="MSVSYKGTISKTHSVFKPEKGRYYIYGALGCPFTHRAILARSLK
KLEPVLGLVLSHWKLDSKGARFLSAPHRPEKYKERFFTATGGIASAKLDESEELGDVN
NDSARLFVDGAFDPVENISRLSELYYLNDPKYPGTKFTVPVLWDSKTRKIVNNESGDI
IRILNSGVFDEFIQSEETNVIDLVPHDLIDEIDKNIKWVHPKINLGVYKVGLAENGKI
YETEVKTLFENLQKMECVLKENYKRLEEQFSGNKQKILAKYFVLGQRLTEADIRLYPS
IIRFDVVYVQHFKCNLKTIRDGFPYLHLWLINLYWNYAEFRFTTDFNHIKLFYIRMEV
SRNKINQFGIVPLGPKPDISRL"
gene 772326..773849
/gene="Cys4"
/locus_tag="P301_G12756"
mRNA 772326..773849
/gene="Cys4"
/locus_tag="P301_G12756"
/product="Cys4p"
CDS 772326..773849
/gene="Cys4"
/locus_tag="P301_G12756"
/note="Similar to YGR155W 'Cystathionine beta-synthase,
catalyzes synthesis of cystathionine from serine and
homocysteine, the first committed step in cysteine
biosynthesis; responsible for hydrogen sulfide generation;
mutations in human ortholog cause homocystinuria'"
/codon_start=1
/product="Cys4p"
/protein_id="EWG91110.1"
/translation="MTKSEQQADSRHNVIDLVGNTPLIALKKLPKALGIKPQIYAKLE
LYNPGGSIKDRIAKSMVEEAEASGRIHPSRSTLIEPTSGNTGIGLALIGAIKGYRTII
TLPEKMSNEKVSVLKALGAEIIRTPTAAAWDSPESHIGVAKKLEKEIPGAVILDQYNN
MMNPEAHYFGTGREIQRQLEDLNLFDNLRAVVAGAGTGGTISGISKYLKEQNDKIQIV
GADPFGSILAQPENLNKTDITDYKVEGIGYDFVPQVLDRKLIDVWYKTDDKPSFKYAR
QLISNEGVLVGGSSGSAFTAVVKYCEDHPELTEDDVIVAIFPDSIRSYLTKFVDDEWL
KKNNLWDDDVLARFDSSKLEASTTKYADVFGNATVKDLHLKPVVSVKETAKVTDVIKI
LKDNGFDQLPVLTEDGKLSGLVTLSELLRKLSINNSNNDNTIKGKYLDFKKLNNFNDV
SSYNENKSGKKKFIKFDENSKLSDLNRFFEKNSSAVITDGLKPIHIVTKMDLLSYLA"
gene 774337..775614
/gene="Pti1"
/locus_tag="P301_G12761"
mRNA 774337..775614
/gene="Pti1"
/locus_tag="P301_G12761"
/product="Pti1p"
CDS 774337..775614
/gene="Pti1"
/locus_tag="P301_G12761"
/note="Similar to YGR156W 'Essential protein that is a
component of CPF (cleavage and polyadenylation factor);
involved in 3' end formation of snoRNA and mRNA; interacts
directly with Pta1p; has similarity to mammalian
Cleavage-Stimulation Factor CstF-64'"
/codon_start=1
/product="Pti1p"
/protein_id="EWG91111.1"
/translation="MTDPRRRTGRHFLTPENLSSTLQITNLPPEWNQDIITSVVAGSG
PVIDIKAKNDPRTGKLTGVLFDYLTSKDCKRAWEILNRIENFPVKIEQIIPPNYKDHL
RETANKNSQKQVLQLNRDSYPFEAGLELPFEMVTEVPIPRRPPPPQAANNTNSVSNNT
NIQFPDILSKASKHLPSFQDGSIIAPDKISQNLSKIPPLQLIEIISNLKILSNQENIQ
KSQLESFLDTNSDITISVTQALLEMGFIDYSVVTKVLKSQVGEAPSLLSSNNTSNSNT
PVSVIRNNTPLHVPSNEVSNNPNNMPLNVAMPMPMSTPPFIPLPLQQQPFGFAPPGPF
MPPAQGPSMGQPVLANQLGQVQQQNISSTEGPSNANKANDSGTINMAKLQLLPENQQD
MIKQVLTLTPAQIQSLPSDQQLMVENFRKEYII"
gene 776233..778842
/gene="Cho2"
/locus_tag="P301_G12771"
mRNA 776233..778842
/gene="Cho2"
/locus_tag="P301_G12771"
/product="Cho2p"
CDS 776233..778842
/gene="Cho2"
/locus_tag="P301_G12771"
/note="Similar to YGR157W Phosphatidylethanolamine
methyltransferase (PEMT), catalyzes the first step in the
conversion of phosphatidylethanolamine to
phosphatidylcholine during the methylation pathway of
phosphatidylcholine biosynthesis"
/codon_start=1
/product="Cho2p"
/protein_id="EWG91112.1"
/translation="MSSCKTTLSEMVGSVTKDRGTINVKARTRSSNVTFKPPVTHDMV
RSLFDPTLKKSLLEKCIALAIISNFFICYWVFQRFGLQFTKYFFLVQYLFWRIAYNLG
IGLVLHYQSHYETLTNCAKTHAIFSKIPHNKDANSNFSTNSNSFSEKFWNFIRKFCQY
EIRSKMPKEYDLFAYPEEINVWLIFRQFVDLILMQDFVTYIIYVYLSIPYSWVQIFNW
RSLLGVILILFNIWVKLDAHRVVKDYAWYWGDFFFLEESELIFDGVFNISPHPMYSIG
YLGYYGLSLICNDYKVLLVSVFGHYSQFLFLKYVENPHIERTYGDGTDSDSQMNSRID
DLISKENYDYSRPLINMGLSFNNFNKLRFTDYFTIGTVAALMLGAIMNARFINLNYLF
ITVFVTKLVSWLFISTILYKQSQSKWFTRLFLENGYTQVYSYEQWQFIYNYYLVLTYT
LMIIYTGLQIWSNFSNINNSQLIFGLILVALQTWCDKETRLAISDFGWFYGDFFLSNY
ISTRKLTSQGIYRYLNHPEAVLGVVGVWGTVLMTNFAVTNIILAVLWTLTNFILVKFI
ETPHVNKIYGKTKRVSGVGKTLLGLKPLRQVSDIVNRIENIIIKSLVDESKNSNGGAE
LLPKNYQDNKEWNILIQEAMDSVATRLSPYCELKIENEQIETNFVLPTPVTLNWKMPI
ELYNGDDWIGLYKVIDTRADREKTRVGSGGHWSATSKDSYMNHGLRHKESVTEIKATE
KYVQGKVTFDTSLLYFENGIYEFRYHSGNSHKVLLISTPFEISLPVLNTTTPELFEKD
LTEFLTKVNVLKDGKFRPLGNKFFGMDSLKQLIKNSIGVELSSEYMRRVNGDAHVISH
RAWDIKQTLDSLA"
gene complement(779060..779812)
/gene="Mtr3"
/locus_tag="P301_G12776"
mRNA complement(779060..779812)
/gene="Mtr3"
/locus_tag="P301_G12776"
/product="Mtr3p"
CDS complement(779060..779812)
/gene="Mtr3"
/locus_tag="P301_G12776"
/note="Similar to YGR158C 'Exosome non-catalytic core
component; involved in 3'-5' RNA processing and
degradation in both the nucleus and the cytoplasm; has
similarity to E. coli RNase PH and to human hMtr3p
(EXOSC6)'"
/codon_start=1
/product="Mtr3p"
/protein_id="EWG91113.1"
/translation="MNVQDRRRLLGPGAAKPMAFSNTTTHVPEKKSTDLTPKGNESEQ
ELSLHTGFIENCNGSALVEARSLGHQTSLISAVYGPRSIRGSFTSQGTISIQLKNGLL
EKYNTNELKEVSSFLMGIFNSVVNLSRYPKSGIDIFVYLTYDKDLTNNPQDDDSQSKM
TSSQISSLIPHCITSITLALADAGIELVDMAGAGEANGTVVSFIKNGEEIVGFWKDDG
DDEDLLECLDRCKEQYNRYRDLMISCLMNQET"
gene complement(780203..781453)
/gene="Nsr1"
/locus_tag="P301_G12781"
mRNA complement(780203..781453)
/gene="Nsr1"
/locus_tag="P301_G12781"
/product="Nsr1p"
CDS complement(780203..781453)
/gene="Nsr1"
/locus_tag="P301_G12781"
/note="Similar to YGR159C Nucleolar protein that binds
nuclear localization sequences, required for pre-rRNA
processing and ribosome biogenesis"
/codon_start=1
/product="Nsr1p"
/protein_id="EWG91114.1"
/translation="MAKTTKVKGNKKEVKASKQAKEEKAKAASSSSSESSSSSSSSSE
SESESESESESSSSSSSSDSESSSSSSSDSESEAETKKEESKDSSSSSSSSDSSSDEE
EEEEKEETKKEESKESSSSDSSSSSSSDSESEKEESNDKKRKSEDAEEEEDEESSNKK
QKNEETEEPATIFVGRLSWSIDDEWLKKEFEHIGGVIGARVIYERGTDRSRGYGYVDF
ENKSYAEKAIQEMQGKEIDGRPINCDMSTSKPAGNNDRAKKFGDTPSEPSDTLFLGNL
SFNADRDAIFELFAKHGEVVSVRIPTHPETEQPKGFGYVQFSNMEDAKKALDALQGEY
IDNRPVRLDFSSPRPNNDGGRGGSRGFGGRGGGRGGNRGFGGRGGARGGRGGFRPSGS
GANTAPLGRSRNTASFAGSKKTFD"
assembly_gap 782090..782240
/estimated_length=151
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(782485..783276)
/gene="Rts3"
/locus_tag="P301_G12806"
mRNA complement(782485..783276)
/gene="Rts3"
/locus_tag="P301_G12806"
/product="Rts3p"
CDS complement(782485..783276)
/gene="Rts3"
/locus_tag="P301_G12806"
/note="Similar to YGR161C Putative component of the
protein phosphatase type 2A complex"
/codon_start=1
/product="Rts3p"
/protein_id="EWG91115.1"
/translation="MIATSRAVNMNKESKHKKAIAKPCRERQTSVTRAMRPAVARDPR
RLSTSSSPSSSPMSAQRRLSREEIINEMEKEQDAIVVRLLREIETLKEENSRLKNQLH
HPVPARRSSPFFEGESAILDDDDCNYGYTLDTPKLKLTDGASRHTVLPLTPKDSMTHI
SHSARRSSRNASISNGTSISDTIFPIETKIHSAPTTNRNLSSADLPHHTLLPRSLSGI
SSSDLTESGALLHDRRRRSSNYSLDGSNSLKADLMAKRFQTGSLK"
gene 784083..784361
/locus_tag="P301_G12811"
mRNA 784083..784361
/locus_tag="P301_G12811"
/product="hypothetical protein"
CDS 784083..784361
/locus_tag="P301_G12811"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91116.1"
/translation="MSGYFNHLSSNAHFANIQADQGFIGDAAGTSSDHGSSGMVDFAL
QLGELSLEEKILKEFTLFQSKNMDLLQETATACPSTNPSLRQSRIQGW"
gene 785455..785528
/locus_tag="P301_G12821"
tRNA 785455..785528
/locus_tag="P301_G12821"
/product="tRNA-Val"
/note="TV(AAC)G2"
gene 786030..788888
/gene="Tif4631"
/locus_tag="P301_G12826"
mRNA 786030..788888
/gene="Tif4631"
/locus_tag="P301_G12826"
/product="Tif4631p"
CDS 786030..788888
/gene="Tif4631"
/locus_tag="P301_G12826"
/note="Similar to YGR162W 'Translation initiation factor
eIF4G, subunit of the mRNA cap-binding protein complex
(eIF4F) that also contains eIF4E (Cdc33p); interacts with
Pab1p and with eIF4A (Tif1p); also has a role in
biogenesis of the large ribosomal subunit'"
/codon_start=1
/product="Tif4631p"
/protein_id="EWG91117.1"
/translation="MTDETAHPTQSASKQESAALKQTGDDQQESQQQRGYTNYNNGSN
YAQKKPYNSNRPHQQRGGKFGPNRYNNRGNYNGGGSFRGGHMGANSSNVPWTGYYNNY
PVYYQPQQMAAAGSAPANPIPVEEKSPVPTKIEITTKSGEHLDLKEQHKAKLQSQERS
TVSPQPESKLKETSDSTSTSTPTPTPSTNDSKASSEENISEAEKTRRNFIEQVKLRKA
ALEKKRKEQLEGSSGNNNIPMKTTPENVEEKGSDKPEVTEKTKPAEEKSAEPEVKQET
PAEEGEQGEKGQIKEESTPKVLTFAERLKLKKQQKEREEKTEGKENKEVPVQEETKSA
IESAPVPPSEQVKEETEVAETEQSNIDESATTPAIPTKSDEAEAEVEAEAGDAGTKIG
LEAEIETTTDETDDGTNTVSHILNVLKDATPIEDVFSFNYPEGIEGPDIKYKKEHVKY
TYGPTFLLQFKDKLNVKADAEWVQSTASKIVIPPGMGRGNRSRDSGRFGNNSSRGHDF
RNTSVRNMDDRANSRTSSKRRSKRMNDDRRSNRSYTSRRDRERGSYRNEEKREDDKPK
EEVAPLVPSANRWVPKFKSKKTEKKLAPDGKTELLDKDEVERKMKSLLNKLTLEMFDA
ISSEILAIANISVWETNGETLKAVIEQIFLKACDEPHWSSMYAQLCGKVVKELNPDIT
DETNEGKTGPKLVLHYLVARCHAEFDKGWTDKLPTNEDGTPLEPEMMSEEYYAAASAK
RRGLGLVRFIGFLYRLNLLTGKMMFECFRRLMKDLTDSPSEETLESVVELLNTVGEQF
ETDSFRTGQATLEGSQLLDSLFGILDNIIQTAKISSRIKFKLIDIKELRHDKNWNSDK
KDNGPKTIQQIHEEEERQRQLKNNSRSNSRRTNNSSNRHSFRRDAPPASKDSFITTRT
YSQRNSQRAPPPKEEPAAPTSTATNMFSALMGESDDEE"
gene 789523..790548
/gene="Gtr2"
/locus_tag="P301_G12836"
mRNA 789523..790548
/gene="Gtr2"
/locus_tag="P301_G12836"
/product="Gtr2p"
CDS 789523..790548
/gene="Gtr2"
/locus_tag="P301_G12836"
/note="Similar to YGR163W 'Putative GTP binding protein
that negatively regulates Ran/Tc4 GTPase cycle; activates
transcription; subunit of EGO and GSE complexes; required
for sorting of Gap1p; localizes to cytoplasm and to
chromatin; homolog of human RagC and RagD'"
/codon_start=1
/product="Gtr2p"
/protein_id="EWG91118.1"
/translation="MSLEATDSKAMVLLMGVRRCGKSSICKVVFHNMQPLDTLYLEST
SNPSLEHFSTLIDLAVMELPGQLNYFEPSYDSERLFKSVGALVYVIDSQDEYINAITN
LAMIIEYAYKVNPSINIEVLIHKVDGLSEDFKVDAQRDIMQRTGEELLELGLDGVQVS
FYLTSIFDHSIYEAFSRIVQKLIPELSFLENMLDNLIQHSKIEKAFLFDVNSKIYVST
DSNPVDIQMYEVCSEFIDVTIDLFDLYKAPVLRNSQKSSDKDNVINPRNELQNVSQLA
NGVIIYLRQMIRGLALVAIIRPNGTDMESCLTVADYNIDIFKKGLEDIWANARASQAK
NSIEDDV"
gene complement(790694..790765)
/locus_tag="P301_G12846"
tRNA complement(790694..790765)
/locus_tag="P301_G12846"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg3);
TR(UCU)G2"
gene 791087..792124
/gene="Mrps35"
/locus_tag="P301_G12851"
mRNA 791087..792124
/gene="Mrps35"
/locus_tag="P301_G12851"
/product="Mrps35p"
CDS 791087..792124
/gene="Mrps35"
/locus_tag="P301_G12851"
/note="Similar to YGR165W Mitochondrial ribosomal protein
of the small subunit"
/codon_start=1
/product="Mrps35p"
/protein_id="EWG91119.1"
/translation="MSYGLTGTSSKLRGTSSIFSWTQVRHFSRRRIAYPFYPFKKLGR
QHPKKHDTNLKTAMRQFLGPKNYKGEYVMNKYFTVPTNHVPNYIKPDLERGQSLEHPV
TKKPLQLRYDGTLGPPPVENKRLQNIFKDRLLQPFPSNPHCKTNYVLSPQLKQSIFEE
ITVEGLSAQQVSQKYGLKIPRVEAIVKLVSVENSWNRRNRVSSDLKTMDETLYRMFPV
FDSDASFKRENLSEIPVPQKTLASRFLTIAESEPFGPVDAAHVLELEPAVETLRNLST
VGEHSSGHQQSTNKNTKVIYGELVEGERSQYKFTNAKVGKVGYRYGSGNRDNKKDRRI
GFNKLGQMVYI"
gene 792486..794168
/gene="Kre11"
/locus_tag="P301_G12856"
mRNA 792486..794168
/gene="Kre11"
/locus_tag="P301_G12856"
/product="Kre11p"
CDS 792486..794168
/gene="Kre11"
/locus_tag="P301_G12856"
/note="Similar to YGR166W 'Subunit of TRAPPII, a
multimeric guanine nucleotide-exchange factor for Ypt1p;
involved in intra-Golgi traffic and the retrograde pathway
from the endosome to Golgi; role in cell wall beta-glucan
biosynthesis and the stress response'"
/codon_start=1
/product="Kre11p"
/protein_id="EWG91120.1"
/translation="MECFVPLRCDLDGSNIEQLRQSHLSRKFIIFDEQLNLWLWFQGN
SQENKRFVLQNMIILINEAQVTRTSTIDDYFTQVENNENLWRLKNDCCSKILFKSNVV
MNNGYNNQIKFVFEYKSVDANFNNQDSLHDPQAKYTLDKYSSEEILPSFEPVYSWSSA
ATKSSKNTNNHLEKNNRATHRVSSKNSEVHEADVSRNPNTFTLKLQYPIFSLLNMRLR
NISLKSEHCILSSLDFQTSKASEQLTKKFIYPQEHNSFLKLNFQEISYKLIDGTSQIE
LDPICPLKVPLTAFSYDSISATFKLVLLPKSTQPHRVKITLAYELELHPNLKLPVRTS
WETEVTLKRSMPISSTSSQYSSNNNNTNHSASFNGAANNFNSGGLANLRLGGVSSSRF
SLGAASTTSLVNSKLSNVKFKFINSNIKVIKGEKFTMRLQIINSSSSPLDLVVYYNNT
INPIPSANNVRNSNGINNCGMNNGTIPNSPLTLENQYQLHNKYRKIAEGIILLSNDYK
IPVVPPRETYFADLRFIGIMSGYYGTLSGLKVLDLNTNELIEVGNGASVLIQ"
assembly_gap 794387..794401
/estimated_length=15
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 794427..795128
/gene="Clc1"
/locus_tag="P301_G12866"
mRNA 794427..795128
/gene="Clc1"
/locus_tag="P301_G12866"
/product="Clc1p"
CDS 794427..795128
/gene="Clc1"
/locus_tag="P301_G12866"
/note="Similar to YGR167W 'Clathrin light chain, subunit
of the major coat protein involved in intracellular
protein transport and endocytosis; thought to regulate
clathrin function; two Clathrin heavy chains (CHC1) form
the clathrin triskelion structural component'"
/codon_start=1
/product="Clc1p"
/protein_id="EWG91121.1"
/translation="MSEKFPPLEDQNIDFTPNDKKNDDTDFLKREAEILGDEFKTEQD
DILETEASPAKDDDEIRDFEEQFPDINSANGAVSSDQNGSATVSSGNDNGEADDDFST
FEGANQSTESVKEDRSEVVDQWKQRRAVEIHEKDLKDEELKKELQDEAIKHIDDFYDS
YNKKKEQQLEDAAKEAEAFLKKRDEFFGQDNTTWDRALQLINQDDADIIGGRDRSKFK
EILLRLKGNAKAPGA"
gene complement(795323..796449)
/locus_tag="P301_G12871"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(796656..797870)
/gene="Pus6"
/locus_tag="P301_G12881"
mRNA complement(796656..797870)
/gene="Pus6"
/locus_tag="P301_G12881"
/product="Pus6p"
CDS complement(796656..797870)
/gene="Pus6"
/locus_tag="P301_G12881"
/note="Similar to YGR169C 'tRNA:pseudouridine synthase,
catalyzes the conversion of uridine to pseudouridine at
position 31 in cytoplasmic and mitochondrial tRNAs;
mutation of Asp168 to Ala abolishes enzyme activity; not
essential for viability'"
/codon_start=1
/product="Pus6p"
/protein_id="EWG91122.1"
/translation="MSTIKVIEVYTQNGLRKVRPYYNRRSAFVKGRWLGKLLIDVLVS
EFKLRPRAYYLDQIRKGTYRLIRDGVPLVPDHLMTTIIKNHDVLETTTHKHEPPVKQW
CSQEVEAEDLPGRIAGFNIVFEDESILVIDKPSGIPVHPTGQFYQNTITELLKFHGVD
ALPCYRLDKITSGLLILAKNSQSAGEIQKSIRSRDMIKIYLARVKGRFPHSELILDNE
NAAETTFEDTSKVTVEMTPIYSIDPKRQFPVGLSASKDAITKFYPIRYFSHADETVVA
CKPITGRTHQIRIHLARLGHPIVNDSVYCSHITKYPERLKFITQFPRWEDQQDLDAEE
LKVRFQKFVDETKNNCQTMETFCPECHTVDLKDPVLSDLELWLHAWKYEEINGKFKFK
TDLPKWAQLDNS"
gene complement(798354..798632)
/locus_tag="P301_G12886"
mRNA complement(798354..798632)
/locus_tag="P301_G12886"
/product="hypothetical protein"
CDS complement(798354..798632)
/locus_tag="P301_G12886"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91123.1"
/translation="MGKRFSESAAKKAAGLARKRDQAHAKQRAQMEQLEAEEASKWEQ
GSRKENAKKLEEEQKRQEKARAKKERDALLTAEEEQLGKGGKGKRKMK"
gene 799106..802522
/gene="Psd2"
/locus_tag="P301_G12891"
mRNA 799106..802522
/gene="Psd2"
/locus_tag="P301_G12891"
/product="Psd2p"
CDS 799106..802522
/gene="Psd2"
/locus_tag="P301_G12891"
/note="Similar to YGR170W Phosphatidylserine decarboxylase
of the Golgi and vacuolar membranes, converts
phosphatidylserine to phosphatidylethanolamine"
/codon_start=1
/product="Psd2p"
/protein_id="EWG91124.1"
/translation="MRIIKGRKRGKNKKPTLILKIHVIQAENIEALKTFNCNPVCFVT
TNTFYSQKTNKLKNSNTHWNQTLRIKLPRNPTSEWLRIIVYDALPTGAPPTTPSRPRT
TTANTSSSTLSNSGLSSHSHSSRNLNVTSKGNQTSTSINSVSSSATPAPSHSSSSLST
TGPGSTHKNRINSYLYLGEAKISLLDLFKRKDTTTSYKFSIEAQRYHLYDMKGGKDQD
SLNCNFLVGDILLGFKLECNVKRTPTFQAFNAWRNELNTYLGRIDRNKARMRSSSSLP
PPLEDMLSNSSAVSGNEIRREKPYSDTDLAHDEEVNAEDEIDAEESIEDMNSSGSICT
ERRYDIDNDTIFDSISEVVSLNDEELDILNDFEEADHPNVPDINVHDIDEDTRISLSS
MITALDEYDIVEPEDVAKLPAVSENDITSVDDEESENQQESDEEFDIYNEDEREDSDF
QSKEYIGSRLLHLQRGKHNKSYANYLYRRAKSNFFISKKEHAMGVVFMHIGAIKNLPA
LRNRLSKTNYEMDPFIVISFGRRVFKTSWRKHTLNPEFNEYAAFEVFPHETNFAFSIK
VVDKDSFSFNDDVAKCELAWFDMLQQQQHENEWIPYEIPLDLTVEPAHAPKQPVLYSS
FKYVSYPFLKKSFWKEAVDTSVNLERLDIIQVMLYLERLGSFTMADSFELFQHFNKSA
WAGQSITRSQLVEGLQSWRKSTNFKRIWTCPRCMRSCKPTRNARRSKLVLENDLITHF
AICTFSKEHKTLKPSYVSSAFASKRWFSKVLIKLTYGKYALGSNNANILVQDRDTGII
IEEKISAHVKLGMRIIYNGKSPESKKFRSLLKTLSIRQGKKFDSTASAKQIEPFIKFH
SLDLSQCRDKDFKTFNEFFYRKLKPGSRLPESNNKEILFSPADSRCTVFPTIQESKEI
WVKGRKFSIKKLANNYNPETFNDNNCSIGIFRLAPQDYHRFHSPCNGTIGKPVYVDGE
YYTVNPMAVRSELDVFGENIRVIIPIDSPQFGKLLYIPIGAMMVGSILLTCKENDVVE
SGQELGYFKFGGSTIIIIIPHNNFMFDSDLVKNSSERIETLVKVGMSIGHTSNVNELK
RIRIKVDDPKKIERIKRTISVSDENAKSTGNVTWEYHTLREMMNKDFAGL"
gene complement(802788..804515)
/gene="Msm1"
/locus_tag="P301_G12896"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 802829..802866
/estimated_length=38
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(804809..805555)
/gene="Yip1"
/locus_tag="P301_G12906"
mRNA complement(804809..805555)
/gene="Yip1"
/locus_tag="P301_G12906"
/product="Yip1p"
CDS complement(804809..805555)
/gene="Yip1"
/locus_tag="P301_G12906"
/note="Similar to YGR172C 'Integral membrane protein
required for the biogenesis of ER-derived COPII transport
vesicles; interacts with Yif1p and Yos1p; localizes to the
Golgi, the ER, and COPII vesicles'"
/codon_start=1
/product="Yip1p"
/protein_id="EWG91125.1"
/translation="MSFYNTSNNANNGGGFYQPSAQFAVPQGSMSFQNTVGSSNTGND
NNLGVAPDPLPVGILHALSTKGYAHEPPLLEEIGINFDHIITKTKMVLIPIRFGSGVP
QEILNDSDLAGPLIFFLLFGLFLLMAGKVHFGYIYGVALFGTISLHNLSKLMSNNDTS
IQTNLQFFNTASILGYCFLPLCFLSLLGIFHGLNNTTGYVVSVLFVIWSTWTSSGFLN
SLLQLQNARLLIAYPLLIFYSVFALMVIFV"
gene 805818..806924
/gene="Rbg2"
/locus_tag="P301_G12911"
mRNA 805818..806924
/gene="Rbg2"
/locus_tag="P301_G12911"
/product="Rbg2p"
CDS 805818..806924
/gene="Rbg2"
/locus_tag="P301_G12911"
/note="Similar to YGR173W 'Protein with a role in
translation; forms a complex with Gir2p; has similarity to
mammalian developmentally regulated GTP-binding protein'"
/codon_start=1
/product="Rbg2p"
/protein_id="EWG91126.1"
/translation="MGIIDKIKAIEEEMARTQKNKATEHHLGLLKGKLARYRQQLLAD
EAGXXXXXXXXXXXXXXXXXXXXXXXXXXVGKSSLLGKITTTKSEIAHYAFTTLTSVP
GVLKYQGAEIQIVDLPGIIYGASQGKGRGRQVVATARTADLVLMVLDATKSEHQRASL
EKELENVGIRLNKEKPNIYYKKKETGGVKVTFTSPPKTNLTEQAIKMILRDYRIHNAE
VLVRDDQCTIDDFIDVINEQHRNYVKCLYVYNKIDAVSLEEVDKLAREPNTVVMSCEM
DLGLQDVVEEIWYQLNLSRVYTKKRGVRPVFDDPLVVRNNSTIGDLCHGIHRDFKDKF
KYALVWGSSAKHSPQKCGLNHRIDDEDVVSLFAK"
assembly_gap 805960..806036
/estimated_length=77
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 807615..807685
/locus_tag="P301_G12916"
tRNA 807615..807685
/locus_tag="P301_G12916"
/product="tRNA-Gly"
/note="TG(GCC)G1"
gene complement(807858..808370)
/gene="Cbp4"
/locus_tag="P301_G12921"
mRNA complement(807858..808370)
/gene="Cbp4"
/locus_tag="P301_G12921"
/product="Cbp4p"
CDS complement(807858..808370)
/gene="Cbp4"
/locus_tag="P301_G12921"
/note="Similar to YGR174C 'Mitochondrial protein required
for assembly of ubiquinol cytochrome-c reductase complex
(cytochrome bc1 complex); interacts with Cbp3p and
function is partially redundant with that of Cbp3p'"
/codon_start=1
/product="Cbp4p"
/protein_id="EWG91127.1"
/translation="MQCAITPREAVIAKQRQYKHYLGMERPLWVRWLKVYAIGGAIIG
SGFLLFKYTTPTDQQLISQLSPELRLQYEREKKLRQSEQQALMKIVKETSQSDDPIWK
TGPLQSPWERNGDNVQSRDHFAKVRAEEVQKEELARIRNELSQLRSETEEKTKEIVQD
KQGKSWWRFW"
assembly_gap 808577..808693
/estimated_length=117
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(808896..810230)
/gene="Erg1"
/locus_tag="P301_G12931"
mRNA complement(808896..810230)
/gene="Erg1"
/locus_tag="P301_G12931"
/product="Erg1p"
CDS complement(808896..810230)
/gene="Erg1"
/locus_tag="P301_G12931"
/note="Similar to YGR175C 'Squalene epoxidase, catalyzes
the epoxidation of squalene to 2,3-oxidosqualene; plays an
essential role in the ergosterol-biosynthesis pathway and
is the specific target of the antifungal drug
terbinafine'"
/codon_start=1
/product="Erg1p"
/protein_id="EWG91128.1"
/translation="MPDRIVGELMQPGGVRALRSLGMIQSINNIEAYPVTGYTVFFNG
EQVDIPYPYKADIPKVEKLKDLVKDGNDKVLEDSTIHIKDYEDDERERGVAFVHGRFL
NNLRNITAQEPNVTRVQGNCIEILKDEKNEVVGAKVDIDGRGKVEFKAHLTFICDGIF
SRFRKELHPDHVPTVGSSFVGMSLFNAKNPAPMHGHVILGSDHMPILVYQISPEETRI
LCAYNSPKVPADIKSWMIKDVQPFIPKSLRPSFDEAVSQGKFRAMPNSYLPARQNDVT
GMCVIGDALNMRHPLTGGGMTVGLHDVVLLIKKIGDLDFSDREKVLDELLDYHFERKS
YDSVINVLSVALYSLFAADSDNLKALQKGCFKYFQRGGDCVNKPVEFLSGVLPKPLQL
TRVFFAVAFYTIYLNMEERGFLGLPMALLEGIMILITAIRVFTPFLFGELIG"
assembly_gap 810287..810476
/estimated_length=190
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(810794..812411)
/gene="Atf2"
/locus_tag="P301_G12941"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 811866..811884
/estimated_length=19
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(813020..815188)
/gene="Pbp1"
/locus_tag="P301_G12951"
mRNA complement(813020..815188)
/gene="Pbp1"
/locus_tag="P301_G12951"
/product="Pbp1p"
CDS complement(813020..815188)
/gene="Pbp1"
/locus_tag="P301_G12951"
/note="Similar to YGR178C 'Component of glucose
deprivation induced stress granules, involved in
P-body-dependent granule assembly; similar to human
ataxin-2; interacts with Pab1p to regulate mRNA
polyadenylation; interacts with Mkt1p to regulate HO
translation'"
/codon_start=1
/product="Pbp1p"
/protein_id="EWG91129.1"
/translation="MKGNFRKRDSSTNSRKGGNSDSNYTNGGVPNQNNSSMFYENPEI
TRNFDDRQDYLLANSIGSDVTVTVTSGVKYTGLLVSCNLESTNGIDVVLRFPRVADSG
VSDSVDDLAKTLGETLLIHGEDVAELELKNIDLSLDEKWENSKAQETTPARTNIEKER
VNGESNEVTKFRTDVDISGSGREIKERKLEKWTPEEGAEHFDINKGKALEDDCASWDQ
FAVNEKKFGVKSTFDEHLYTTKINKDDPNYSKRLQEAERIAKEIESQGTSGNIHIAED
RGIIIDDSGLDEEDLYSGVDRRGDELLAALKSNSKPNSNKGNRYVPPTLRQQPHHMDP
AIISSSNSNKNENAVSTDTSTPAAAGAPEGKPPQKTSKNKKSLSSKEAQIEELKKFSE
KFKVPYDIPKDMPEVLKRSNSTLKSNSSLPPKPISKTPSAKTVSPTTQISAGKSESRR
SGSNISQGQSSTGHTTRSSTSSRRRNHGSFFGAKNPHTNDAKRVLFGKSFNMFIKSKE
AHDEKKKGDDASENMEPFFIEKPYFTAPTWLNTIEESYKTFFPDEDTAIQEAQTRFQQ
RQLNSMGNAVPGMNPAMGMNMGGMMGFPMGGPSASPNPMMNGFAAGSMGMYMPFQPQP
MFYHPSMPQMMPVMGSNGAEEGGGNISPHVPAGFMAAGPGAPMGAFGYPGGIPFQGMM
GSGPSGMPANGSAMHSHGHSRNYHQTSHHGHHNSSTSGHK"
gene complement(815647..816867)
/gene="Okp1"
/locus_tag="P301_G12961"
mRNA complement(815647..816867)
/gene="Okp1"
/locus_tag="P301_G12961"
/product="Okp1p"
CDS complement(815647..816867)
/gene="Okp1"
/locus_tag="P301_G12961"
/note="Similar to YGR179C 'Outer kinetochore protein,
required for accurate mitotic chromosome segregation;
component of the kinetochore sub-complex COMA (Ctf19p,
Okp1p, Mcm21p, Ame1p) that functions as a platform for
kinetochore assembly'"
/codon_start=1
/product="Okp1p"
/protein_id="EWG91130.1"
/translation="MAADRDNFLQNIENDSINNGQAMDLSPNRSSSESDSSILMNVND
IKTLRLDVAPEAKSTQSKKSLFYENSDDAEEGEIEERTNKEEGQYHHKGSKQLRFEVG
KESTGKLQSHLSDGSATSGEGNVRPWEFRKVIQAEYRERLPRNYELKHWKKPSKIMIG
SILRLLETNTVSALDSVFEKYEKEMNQMTHGDNNEVKRIYSKKERLLEIILTKIKKKL
IQAKFPSRISERDLDIEYIYSKRQFIQNRYSQELQNNERLEAILSREQNLLEETRKLC
MNLKTNNKKRLTEKLIQKDLHPVLNKAMEYTYGLESTNGFMHPDGPVTFRNDSHELNL
MLNDPIKSTADVRLDKEEVLSLLPSLKEYTKKSKELKETMGQMISNSHEEEIKEVFVP
HHESHQDKTEEDIH"
gene complement(817236..818273)
/gene="Rnr4"
/locus_tag="P301_G12966"
mRNA complement(817236..818273)
/gene="Rnr4"
/locus_tag="P301_G12966"
/product="Rnr4p"
CDS complement(817236..818273)
/gene="Rnr4"
/locus_tag="P301_G12966"
/note="Similar to YGR180C 'Ribonucleotide-diphosphate
reductase (RNR), small subunit; the RNR complex catalyzes
the rate-limiting step in dNTP synthesis and is regulated
by DNA replication and DNA damage checkpoint pathways via
localization of the small subunits'"
/codon_start=1
/product="Rnr4p"
/protein_id="EWG91131.1"
/translation="MEAHNQFLKTFQKERHDMKEAEKDEILLMENSRRFVMFPIKYHE
IWAAYKKVEASFWTAEEIELAKDTEDFQKLTDDQKTYIGNLLALSISSDNLVNKYLIE
NFSAQLQNPEGKSFYGFQIMMENIYSEVYSMMVDAFFKDPKNIPLFKEIANLPEVKHK
TAFIERWISNDDSLYAERLVAFAAKEGIFQAGNYASMFWLTDKKIMPGLAMANRNICR
DRGAYTDFSCLLFAHLRTKPNPKIIEKIITEAVEIEKEYYSNSLPVEKFGMDLKSIHT
YIEFVADGLLQGFGNEKYYNAVNPFEFMEDVATAGKTTFFEKKVSDYQKASDMSKSAT
PSKEINFDDDF"
gene complement(819348..819461)
/locus_tag="P301_G12976"
tRNA complement(819348..819461)
/locus_tag="P301_G12976"
/product="tRNA-Leu"
/note="TL(CAA)G3"
gene 820257..820574
/gene="Tim13"
/locus_tag="P301_G12981"
mRNA 820257..820574
/gene="Tim13"
/locus_tag="P301_G12981"
/product="Tim13p"
CDS 820257..820574
/gene="Tim13"
/locus_tag="P301_G12981"
/note="Similar to YGR181W Mitochondrial intermembrane
space protein, forms a complex with Tim8p that delivers a
subset of hydrophobic proteins to the TIM22 complex for
insertion into the inner membrane"
/codon_start=1
/product="Tim13p"
/protein_id="EWG91132.1"
/translation="MGLSSIFGGGAPSQQKEAATTAKTTPNPIAKELKNQIAQELAVA
NATELVNKISENCFEKCLTSPYATRNDACIDQCLAKYMRSWNVISKAYISRIQNASAS
GEI"
gene complement(821033..821446)
/gene="Qcr9"
/locus_tag="P301_G12991"
mRNA complement(join(821033..821230,821444..821446))
/gene="Qcr9"
/locus_tag="P301_G12991"
/product="Qcr9p"
CDS complement(join(821033..821230,821444..821446))
/gene="Qcr9"
/locus_tag="P301_G12991"
/note="Similar to YGR183C 'Subunit 9 of the ubiquinol
cytochrome-c reductase complex, which is a component of
the mitochondrial inner membrane electron transport chain;
required for electron transfer at the ubiquinol oxidase
site of the complex'"
/codon_start=1
/product="Qcr9p"
/protein_id="EWG91133.1"
/translation="MSFSSLYKTFFKRNAVFVGTIFAGAFVFQTVFDTAITSWYENHN
KGKLWKDVKARIAAGDGDDDDE"
gene complement(821870..827722)
/gene="Ubr1"
/locus_tag="P301_G12996"
mRNA complement(821870..827722)
/gene="Ubr1"
/locus_tag="P301_G12996"
/product="Ubr1p"
CDS complement(821870..827722)
/gene="Ubr1"
/locus_tag="P301_G12996"
/note="Similar to YGR184C 'E3 ubiquitin ligase
(N-recognin), forms heterodimer with Rad6p to ubiquitinate
substrates in the N-end rule pathway; regulates peptide
transport via Cup9p ubiquitination; mutation in human UBR1
causes Johansson-Blizzard Syndrome (JBS)'"
/codon_start=1
/product="Ubr1p"
/protein_id="EWG91134.1"
/translation="MSVADDDLGSLQGHIRRTLRSIHNLPYFRYTRGPTERADMSRAL
KEFIYRYLYFVISNNGENLPTLFNAHPKQKLSNPELTVFPESLEDAVDIDKITSQQTI
PFYKIDESRIGDVHKHTGRNCGRKFKIGEPLYRCHECGCDDTCVLCIHCFNPKDHVNH
HVCTDICTEFTSGICDCGDEEAWNSPLHCKAEEQENDISEDPATNADIKEEDVWNDSV
NIALVELVLAEVFDYFIDVFNQNIEPLPTIQKDITIKLREMTQQGKMYERAQFLNDLK
YENDYMFDGTTTAKTSPSNSPEASPSLAKIDPENYTVIIYNDEYHNYSQATTALRQGV
PDNVHIDLLTSRIDGEGRAMLKCSQDLSSVLGGFFAVQTNGLSATLTSWSEYLHQETC
KYIILWITHCLNIPNSSFQTTFRNMMGKTLCSEYLNATECRDMTPVVEKYFSNKFDKN
DPYRYIDLSILADGNQIPLGHHKILPESSTHSLSPLINDVETPTSRTYSNTRLQHILY
FDNRYWKRLRKDIQNVIIPTLASSNLYKPIFCQQVVEIFNHITRSVAYMDREPQLTAI
RECVVQLFTCPTNAKNIFENQSFLDIVWSIIDIFKEFCKVEGGVLIWQRVQKSNLTKS
YSISFKQGLYTVETLLSKVHDPNIPLRPKEIISLLTLCKLFNGAWKIKRKEGEHVLHE
DQNFISYLEYTTSIYSIIQTAEKVSEKSKDSIDSKLFLNAIRIISSFLGNRSLTYKLI
YDSHEVIKFSVSHERVAFMNPLQTMLSFLIEKVSLKDAYEALEDCSDFLKISDFSLRS
VVLCSQIDVGFWVRNGMSVLHQASYYKNNPELGSYSRDIHLNQLAILWERDDIPRIIY
NILDRWELLDWFTGEVDYQHTVYEDKISFIIQQFIAFIYQILTERQYFKTFSSLKDRR
MDQIKNSIIYNLYMKPLSYSKLLRSVPDYLTEDTTEFDEALEEVSVFVEPKGLADNGV
FKLKASLYAKVDPLKLLNLENEFESSATIIKSHLAKDKDEIAKVVLIPQVSIKQLDKD
ALNLGAFTRNTVFAKVVYKLLQVCLDMEDSTFLNELLHLVHGIFRDDELINGKDSIPG
AYLSKPICNLLLSIANAKSDVFSESIVRKADYLLEKMIMKKPNELFESLIASFGNQYV
NDYKNKKLRQGVNLQETEKERKRRLAKKHQARLLAKFNNQQTKFMKEHESEFDEQDND
VDMVGEKVYESEDFTCALCQDSSSTDFFVIPAYHDHSPIFRPGNIFNPNEFMPMLDGF
YNDDEKQAYIDDDVLEALKENGSCGSRKVFVSCNHHIHHNCFKRYVQKKRFSSNAFIC
PLCQTFSNCTLPLCQTSKANTGLSLDMFLESELSLDTLSRLFKPFTEENYRTINSIFS
LMISQCQGFDKAVRKRANFSHKDVSLILSVHWANTISMLEIASRLEKPHSISFFRSRE
QKYKTLKNILVCIMLFTFVIGKPSMEFEPYPQQPDTVWNQNQLFQYIVRSALFSPVSL
RQTVTEALTTFSRQFLRDFLQGLSDAEEVTKLYAKASKIGDVLKVSEQMLFALRTISD
VRMEGLDSESIIYDLAYTFLLKSLLPTIRRCLVFIKVLHELVKDSENETLVINGHEVE
EELEFEDTAEFVNKALKMITEKESLVDLLTTQESVVSHPYLENIPYEYCGIIKLIDLS
KYLNTYVTQSKEIKLREERSQHMKNADNRLDFKICLTCGVKVHLRADRHEMTKHLNKN
CFKPFGAFLMPNSSEVCLHLTQPPSNIFISAPYLNSHGEVGRNAMRRGDLTTLNLKRY
EHLNRLWINNEIPGYISRVMGDEFRVTILSNGFLFAFNREPRPRRIPPTDEDDEDMEE
GEDGFFTEGNDEMDVDDETGQAANLFGVGAEGIAGGGVRDFFQFFENFRNTLQPQGNG
DDDAPQNPPPILQFLGPQFDGATIIRNTNPRNLDEDDSDDNDDSDEREIW"
gene complement(828305..829489)
/gene="Tys1"
/locus_tag="P301_G13006"
mRNA complement(828305..829489)
/gene="Tys1"
/locus_tag="P301_G13006"
/product="Tys1p"
CDS complement(828305..829489)
/gene="Tys1"
/locus_tag="P301_G13006"
/note="Similar to YGR185C 'Cytoplasmic tyrosyl-tRNA
synthetase, required for cytoplasmic protein synthesis;
interacts with positions 34 and 35 of the tRNATyr
anticodon; mutations in human ortholog YARS are associated
with Charcot-Marie-Tooth (CMT) neuropathies'"
/codon_start=1
/product="Tys1p"
/protein_id="EWG91135.1"
/translation="MSSAATVDPNEAFGLITKNLQEVLNPQIIKDVLEVQKRHLKLYW
GTAPTGRPHCGYFVPMTKLADFLKAGCEVTVLLADLHAFLDNMKAPLEVVNYRAKYYE
LTIKAILRSINVPIEKLKFVVGSSYQLTPDYTMDIFRLSNIVSQNDAKRAGADVVKQV
ANPLLSGLIYPLMQALDEQFLDVDCQFGGVDQRKIFVLAEENLPSLGYKKRAHLMNPM
VPGLAQGGKMSASDPNSKIDLLEEPKQVKKKINSAFCSPGNVEENGLLSFVQYVIAPI
QELKFGTNHFEFFIDRPEKFGGPITYKSFEEMKLAFKEEKLSPPDLKIGVADAINELL
EPIRQEFADNKEFQEASEKGYPVATPQKSKKAKKPKNKGTKYPGATKTNEIATKLEET
KL"
gene 829743..831950
/gene="Tfg1"
/locus_tag="P301_G13011"
mRNA 829743..831950
/gene="Tfg1"
/locus_tag="P301_G13011"
/product="Tfg1p"
CDS 829743..831950
/gene="Tfg1"
/locus_tag="P301_G13011"
/note="Similar to YGR186W 'TFIIF (Transcription Factor II)
largest subunit; involved in both transcription initiation
and elongation of RNA polymerase II; homologous to human
RAP74'"
/codon_start=1
/product="Tfg1p"
/protein_id="EWG91136.1"
/translation="MSRRNPPGSRNGGGPTNASPFIKRDRMRRNFLRMRMGQNGSNSS
SPGVPNGDNSRGSLVKKDDPEYAEEREKMLLQIGVEADAGRSNVKVKDEDPNEYNEFP
LRAIPKEDLENMRTHLLKFQSKKKINPVTDFHLPVRLHRKDTRNLQFQLTRAEIVQRQ
KEISEYKKKAEQERSTPNSGGMNKSGTVSLNTTVKDGSQTPTVDSVTKDNTANSVNSS
IPTVTGSSVPPASPTTVSAVESHGLSNGSTSAANGLDGNASTANLANGRPLVTKLEDA
GPAEDPTKVGMVKYDGKEVTNEPEFEEGTMDPLADVAPDGGGRAKRGNLRRKTRQLKV
LDENAKKLRFEEFYPWVMEDFDGYNTWVGSYEAGNSDSYVLLSVEDDGSFTMIPADKV
YKFTARNKYATLTIDEAEKRMDKKSGEVPRWLMKHLDNIGTTTTRYDRTRRKLKAVAD
QKAMDEDDRDDNSEVELDYDEEFADDEEAPIIDGNEQENKESEQRIKKEMLQANAMGL
RDEEAPSENEEDELFGEKKIDEDGERIKKALQKTELAALYSSDENEINPYLSESDIEN
KENESPVKKEEDSDTLSKSKRSSPKKQQKKATNAHVHKEPTLRVKSIKNCVIILKGGK
KILKSFPEGEWNPQTTKAVDSSNNASNTVPSPIKQEEGLNSTVAEREETPAPTITEKD
IIEAIGDGKVNIKEFGKFIRRKYPGAENKKLMFAIVKKLCRKVGNDHMELKKE"
gene complement(832202..833385)
/gene="Hgh1"
/locus_tag="P301_G13016"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(834014..837079)
/gene="Bub1"
/locus_tag="P301_G13021"
mRNA complement(834014..837079)
/gene="Bub1"
/locus_tag="P301_G13021"
/product="Bub1p"
CDS complement(834014..837079)
/gene="Bub1"
/locus_tag="P301_G13021"
/note="Similar to YGR188C 'Protein kinase involved in the
cell cycle checkpoint into anaphase; forms complex with
Mad1p and Bub3p crucial to preventing cell cycle
progression into anaphase in the presence of spindle
damage; CDC28-mediated phosphorylation at Bub1p-T566 is
important for its degradation in anaphase and adaptation
of the checkpoint to prolonged mitotic arrest, but not to
its kinase activity or chromosome segregation function;
associates with centromere DNA via Skp1p'"
/codon_start=1
/product="Bub1p"
/protein_id="EWG91137.1"
/translation="MNLDLGSTVRGYESDKDTFPQSKGVSSSQKEQHSQLNQTKIAYE
QRLLNDLEDMDDPLDLFLDYMIWISTSYIEVDSESGQEVLRSTMERCLIYIQDMETYR
NDPRFLKIWIWYINLFLSNNFHESENTFKYMFNKGIGTKLSLFYEEFSKLLENAQFFL
EAKVLLELGAENNCRPYNRLLRSLSNYEDRLREMNIVENQNSVPDSRERLKGRLNYRT
APFFIRKFLTSSLMTDDKENRANLNSNVGVGKSAPNVYQDSIVVADFKSETERLNLNS
SKQPSNQRLKNGNKKTSIYADQKQSNNPVYKLINTPGRKPERIVFNFNLIYPENDEEF
NTEEILAMIKGLYKVQRRGKKHTEDYTSDKNRKKRKLDVLVERRQDLPSSQPPVVPKS
TRIEVFKDDDNPSQSTHHKNTQVQVQTTTSILPLKPVVDGNLAHETPVKPSLTSNASR
SPTVTAFSKDAINEVFSMFNQHYSTPGALLDGDDTTTSKFNVFENFTQEFTAKNIEDL
TEVKDPKQETVSQQTTSTNETNDRYERLSNSSTRPEKTDYMTPIKETTETDVVPIIQT
PKEQIRIGDKKSGDNTETQTQLTSTTIQSSPFLTQPEPQAEKLLQTAEHSEKSKEHYP
TIIPPFTKIKNQPPVIIENPLSNNLRAKFLSEISPPLFQYNTFYNYNQELKMSSLLKK
IHRVSRNENKNPIVDFKKTGDLYCIRGELGEGGYATVYLAESSQGHLRALKVEKPASV
WEYYIMSQVEFRLRKSTILKSIINASALHLFLDESYLVLNYASQGTVLDLINLQREKA
IDGNGIMDEYLCMFITVELMKVLEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMR
NGEDGWENKGIYLIDFGRSFDMTLLPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYY
GLAGVIHSMLFGKFIETIQLQNGRCKLKNPFKRYWRKEIWAVIFDLLLNSGQASNQAL
PMTEKIVEIRNLIESHLEQHAENHLRNVILSIEEELSHFQYKGKPSRRF"
gene 838364..838436
/locus_tag="P301_G13026"
tRNA 838364..838436
/locus_tag="P301_G13026"
/product="tRNA-Lys"
/note="TK(CUU)G3"
gene complement(838637..840145)
/gene="Crh1"
/locus_tag="P301_G13031"
mRNA complement(838637..840145)
/gene="Crh1"
/locus_tag="P301_G13031"
/product="Crh1p"
CDS complement(838637..840145)
/gene="Crh1"
/locus_tag="P301_G13031"
/note="Similar to YGR189C 'Chitin transglycosylase that
functions in the transfer of chitin to beta(1-6) and
beta(1-3) glucans in the cell wall; similar and
functionally redundant to Utr2; localizes to sites of
polarized growth; expression induced by cell wall stress'"
/codon_start=1
/product="Crh1p"
/protein_id="EWG91138.1"
/translation="MKVLDLLTVLSASSLLSTFAAAESTATADSTTAASSTASCNPLK
TTGCTPDTALATSFSEDFSSSSKWFTDLKHAGEIKYGSDGLSMTLAKRYDNPSLKSNF
YIMYGKLEVILKAANGTGIVSSFYLQSDDLDEIDIEWVGGDNTQFQSNFFSKGDTTTY
DRGEFHGVDTPTDKFHNYTLDWAMDKTTWYLDGESVRVLSNTSSEGYPQSPMYLMMGI
WAGGDPDNAAGTIEWAGGETNYNDAPFTMYIEKVIVTDYSTGKKYTYGDQSGSWESIE
ADGGSIYGRYDQAQEDFAVLANGGSISSSSTSSSTVSSSASSTVSSTVSSSVSSTVSS
SSSVSSSSSTSPSSSTATSSKTLASSSATTSSSISSLEKQSSSSSKKTVASSSTSESI
ISSTKTPATVSSTPATVSSTTRSTVAPTTQQSSVSSDSPVQDKGGVATSSNDVTSSTT
QISSKYTSTIQSSSSEASSTNSVQISNGADLAQSLPREGKLFSVLVALLALL"
gene complement(840661..840766)
/locus_tag="P301_G13036"
tRNA complement(840661..840766)
/locus_tag="P301_G13036"
/product="tRNA-Trp"
/note="TW(CCA)G2"
assembly_gap 840958..841173
/estimated_length=216
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 842382..844193
/gene="Hip1"
/locus_tag="P301_G13056"
mRNA 842382..844193
/gene="Hip1"
/locus_tag="P301_G13056"
/product="Hip1p"
CDS 842382..844193
/gene="Hip1"
/locus_tag="P301_G13056"
/note="Similar to YGR191W High-affinity histidine
permease, also involved in the transport of manganese
ions"
/codon_start=1
/product="Hip1p"
/protein_id="EWG91139.1"
/translation="MPRNPLKKEYWADVVDGFKPATSPAFENEKESTTFVTELTSKTD
SAFPLSSKDSPGINQTTNDITSSDRFRRNEDTEQEDINNTNLSKDLSVRHLLTLAVGG
AIGTGLYVNTGAALSTGGPASLVIDWVIISTCLFTVINSLGELSAAFPVVGGFNVYSM
RFIEPSFAFAVNLNYLAQWLVLLPLELVAASITIKYWNDKINSDAWVAIFYATIALAN
MLDVKSFGETEFVLSMIKILSIIGFTILGIVLSCGGGPHGGYIGGKYWHDPGAFVGHS
SGTQFKGLCSVFVTAAFSYSGIEMTAVSAAESKNPRETIPKAAKRTFWLITASYVTIL
TLIGCLVPSNDPRLLNGSSSVDAASSPLVIAIENGGIKGLPSLMNAIILIAVVSVANS
AVYACSRCMVAMAHIGNLPKFLNRVDKKGRPMNAILLTLFFGLLSFVAASDKQAEVFT
WLSALSGLSTIFCWMAINLSHIRFRQAMKVQERSLDELPFISQTGVKGSWYGFIVLFL
VLIASFWTSLFPLGGSGASAESFFEGYLSFPILIVCYVGHKLYTRNWTLMVNLEDMDL
DTGRKQVDLTLRREEMRIERETLAKRSFITRFLHFWC"
assembly_gap 844241..844369
/estimated_length=129
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(844774..845772)
/gene="Tdh3"
/locus_tag="P301_G13061"
mRNA complement(844774..845772)
/gene="Tdh3"
/locus_tag="P301_G13061"
/product="Tdh3p"
CDS complement(844774..845772)
/gene="Tdh3"
/locus_tag="P301_G13061"
/note="Similar to YGR192C 'Glyceraldehyde-3-phosphate
dehydrogenase, isozyme 3, involved in glycolysis and
gluconeogenesis; tetramer that catalyzes the reaction of
glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate;
detected in the cytoplasm and cell wall'"
/codon_start=1
/product="Tdh3p"
/protein_id="EWG91140.1"
/translation="MVRVAINGFGRIGRLVMRIALSRPNVEVVALNDPFITNDYAAYM
FKYDSTHGRYAGEVSHDDKHIIVDGKKIATYQERDPANLPWGSSNVDIAIDSTGVFKE
LDTAQKHIDAGAKKVVITAPSSTAPMFVMGVNEDKYTSDLKIVSNASCTTNCLAPLAK
VINDAFGIEEGLMTTVHSLTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKV
LPELQGKLTGMAFRVPTVDVSVVDLTVKLDKETTYDEIKKVVKAAAEGKLKGVLGYTE
DAVVSSDFLGDSHSSIFDASAGIQLSPKFVKLVSWYDNEYGYSTRVVDLVEHVAKA"
gene complement(846473..847705)
/gene="Pdx1"
/locus_tag="P301_G13066"
mRNA complement(846473..847705)
/gene="Pdx1"
/locus_tag="P301_G13066"
/product="Pdx1p"
CDS complement(846473..847705)
/gene="Pdx1"
/locus_tag="P301_G13066"
/note="Similar to YGR193C Dihydrolipoamide dehydrogenase
(E3)-binding protein (E3BP) of the mitochondrial pyruvate
dehydrogenase (PDH) complex, plays a structural role in
the complex by binding and positioning E3 to the
dihydrolipoamide acetyltransferase (E2) core"
/codon_start=1
/product="Pdx1p"
/protein_id="EWG91141.1"
/translation="MLSAISKVSTLKSCTRYLTKCNYHASAKLLAVKTFSMPAMSPTM
EKGGIVSWKYKVGEPFSAGDVILEVETDKSQIDVEALDDGKLAKILKDEGSKDVDVGE
PIAYIADVDDDLATIKLPQEANTANAKSIEIKKPSADSTEATQQHLKKATVTSIKTVD
GSQANLEQTLLPSVSLLLAENNISKEKALKEITPSGSNGRLLKGDVLAYLGKIPQDSV
NKVTEFIKKNERLDLSNIKPIQLKPKIAEQAQTKAADKPKITPVEFEEQLVFHAPASI
PFDKLSESLNSFMKEAYQFSHGTPLMDTNSKYFDPIFEDLVTLSPREPRFKFSYDLMQ
IPKANNMQDTYGQEDIFDLLTGSDATASSVRPVEKNLPEKNEYILALNVSVNNKKFND
AEAKAKRFLDYVRELESF"
gene complement(848035..849837)
/gene="Xks1"
/locus_tag="P301_G13071"
mRNA complement(848035..849837)
/gene="Xks1"
/locus_tag="P301_G13071"
/product="Xks1p"
CDS complement(848035..849837)
/gene="Xks1"
/locus_tag="P301_G13071"
/note="Similar to YGR194C 'Xylulokinase, converts
D-xylulose and ATP to xylulose 5-phosphate and ADP; rate
limiting step in fermentation of xylulose; required for
xylose fermentation by recombinant S. cerevisiae strains'"
/codon_start=1
/product="Xks1p"
/protein_id="EWG91142.1"
/translation="MLCSVIQRQTREVSNTMSLDSYYLGFDLSTQQLKCLAINQDLKI
VHSETVEFEKDLPHYNTKKGVYIHGDTIECPVAMWLEALDLVLSKYREAKFPLNKVMA
VSGSCQQHGSVYWSSQAESLLEQLNKKPEKDLLHYVSSVAFARQTAPNWQDHSTAKQC
QEFEECIGGPEKMAQLTGSRAHFRFTGPQILKIAQLEPEAYEKTKTISLVSNFLTSIL
VGHLVELEEADACGMNLYDIRERKFSDELLHLIDSSSKDKTIRQKLMRAPMKNLIAGT
ICKYFIEKYGFNTNCKVSPMTGDNLATICSLPLRKNDVLVSLGTSTTVLLVTDKYHPS
PNYHLFIHPTLPNHYMGMICYCNGSLARERIRDELNKERENNYEKTNDWTLFNQAVLD
DSESSENELGVYFPLGEIVPSVKAINKRVIFNPKTGMIEREVAKFKDKRHDAKNIVES
QALSCRVRISPLLSDSNASSQQRLNEDTIVKFDYDESPLRDYLNKRPERTFFVGGASK
NDAIVKKFAQVIGATKGNFRLETPNSCALGGCYKAMWSLLYDSNKIAVPFDKFLNDNF
PWHVMESISDVDNENWDRYNSKIVPLSELEKTLI"
gene 850847..851587
/gene="Ski6"
/locus_tag="P301_G13076"
mRNA 850847..851587
/gene="Ski6"
/locus_tag="P301_G13076"
/product="Ski6p"
CDS 850847..851587
/gene="Ski6"
/locus_tag="P301_G13076"
/note="Similar to YGR195W 'Exosome non-catalytic core
component; involved in 3'-5' RNA processing and
degradation in both the nucleus and the cytoplasm; has
similarity to E. coli RNase PH and to human hRrp41p
(EXOSC4)'"
/codon_start=1
/product="Ski6p"
/protein_id="EWG91143.1"
/translation="MSILEIYSPEGLRLDGRRWNELRRFESSINTHPHAADGSSYMEQ
GNNKIITLVKGPKEPRLKSQMDTSKALLNVSVNITKFSKFERSKSSHKNERRVLEIQT
SLVRMFEKNVMLNIYPRTVIDIEIHVLEQDGGIMGSLINGITLALIDAGISMFDYISG
ISVGLYDTTPLLDTNSLEENAMSTVTLGVVGKSEKLSLLLVEDKIPLDRLENVLAIGI
AGAHRVRDLMDEELRKHAQKRVSNASVR"
gene complement(851698..854151)
/gene="Fyv8"
/locus_tag="P301_G13081"
mRNA complement(851698..854151)
/gene="Fyv8"
/locus_tag="P301_G13081"
/product="Fyv8p"
CDS complement(851698..854151)
/gene="Fyv8"
/locus_tag="P301_G13081"
/note="Similar to YGR196C Protein of unknown function,
required for survival upon exposure to K1 killer toxin"
/codon_start=1
/product="Fyv8p"
/protein_id="EWG91144.1"
/translation="MSTEQVGRKKSYRWVSASQASYDGAGWDSSDEYDYSSEDGTKGS
EIHKQKISNLPSLPKLNYTDVSGEHDENTGENKDSNDNNVSKSDISPSDKEVGYLSDG
VPKLMASRESVELQAKKSSEHSKSDYLSSTASLKSPSENKKSPHTNRAVNEDLDNLIE
QISREMTPEIRQTSDFRRDSDSCDEIQNEAPLGEAVPSSSSPVEEDEKSHSLGVSMDT
NEADTTFNTPTRNGNEHLSSDGDVSEQKDDEFKVSERGYLADILPAEKEENLQQEDDG
EVESSGALEKKEKSEEKTSIRNRNSTSSGQDKVAKPKPVANETKTSDNGYRNSFFNDY
QHSSDSEEDDNNEGNSGSSDDDNRSSVSDKHADINQQSKQLDTTDDDALSYTESIKYS
TNETEEEDNEDNESIEDKNEDNESIEDENEDTDSYKFSNREKGSILLTSDEEEEEKGM
SSDSDEGSLKAPKSGYFSKMIGNDDKGDSALQPNQIDTIENTNLSNSGSELENSDGSD
EEDHINEDKVLEESSVKDSTDVDSWKPDSEALRSGFVQDTANKKAPPGYVIDSNGKLV
DLTPASMKPRVVSTYSEMESTWDAFPSKGEDDDLETIRDTKTIYDNNTIYNVPGLIGN
QSNLPPLPMDAQEQLNAGNDNSTTDNDNSNNTANDLAARSASFKSENRTVSQGEMTSV
HEPSTEEMAKLGQQNNLPKLDMNKLLNSKTSHAGKIEQLRNYKRELDGYDTGIQTWIN
YTLKSSSNKDKDFIAEEYKQHSHVREAYANADDLSKKHTVINTVASVNQNVTHLRRKV
FQHSMKPKDLFASIGKKKL"
gene complement(854463..856106)
/gene="Sng1"
/locus_tag="P301_G13086"
mRNA complement(854463..856106)
/gene="Sng1"
/locus_tag="P301_G13086"
/product="Sng1p"
CDS complement(854463..856106)
/gene="Sng1"
/locus_tag="P301_G13086"
/note="Similar to YGR197C 'Protein involved in resistance
to nitrosoguanidine (MNNG) and 6-azauracil (6-AU);
expression is regulated by transcription factors involved
in multidrug resistance'"
/codon_start=1
/product="Sng1p"
/protein_id="EWG91145.1"
/translation="MTKSVGDEESRYIEDPSFAAAAAFTGGRDGVSYSNQRFAEGSGH
SSDLAKSLEDYRPPDEKPSSLSSVGEGGANEEEKGGNDGGPLARIQTGLFSPRLRNHR
KKILSKFVLNNFFIACVCVSLISIYWGACYGTDRYFFKVKNIVVLQDAPSNTSVQSIS
AIIPSLLASVPGTWHIYNATSFHRKFGTTNSTEIDRKIVDLIYDERYWLALNVKPNAT
DTLYNSLISQDANSEFNSSIFFESVFESGRDPSSVKSTILPLMQQLEVRLQKYYVKEY
LPSLMSNITSNDRDLNINMENWAIAGQLLFTYKDYRPFADRILMAPLQVGLIYCILLT
VLQLSLYGKLHGEMARVLKPKHILIYRLLISWATYFLLSIGFCTVSAIFRIDFTPAFG
RGGFVVYWMSTWLVMMAVGGANENVLSLVIAYCPPYLSIWLMTWIILNISASFYPMVL
NNEFYRYGYIMPIHNAVDIYKVIFLNLTKRKMGRNYGILVAWVALNTSLMPFCMKFAG
KKMQKNAMQAAEAAVAAATQRASRPAEANTDKNNNPPGN"
gene 856661..859114
/gene="Ypp1"
/locus_tag="P301_G13091"
mRNA 856661..859114
/gene="Ypp1"
/locus_tag="P301_G13091"
/product="Ypp1p"
CDS 856661..859114
/gene="Ypp1"
/locus_tag="P301_G13091"
/note="Similar to YGR198W 'Cargo-transport protein
involved in endocytosis; interacts with
phosphatidylinositol-4-kinase Stt4; GFP-fusion protein
localizes to the cytoplasm; YGR198W is an essential gene'"
/codon_start=1
/product="Ypp1p"
/protein_id="EWG91146.1"
/translation="MPNSNVRIPPTVPWKIIDVVDQALRARLLGGSTFNSGFDSLDSV
LNLQFRLHYHVIGSNGPAKPVCDVLLKESQNLEKNMSMMEELNDYPEITKLVEKILFN
CLGILFFHRGQFQESQRCLLHSLKIHNNTASQRTALMEQYDRYLILENLYYRGLVSQD
INIMQNVFYKGLLAHVDTIPPESNGLLFEYISLIVAKLRFNQIQDLAENFKTTVENPF
ILFLYMIKKFQSPLKKHIDNDDLYLKFGQNVLLKAKFPTASETNDEALEHFNVFLQYY
FKFTHIKKIKVTPSWYNFIISSMEKTFQSIEVSKTAMFLFQNLSDNSNDEIKKKTFKR
ESILNFVNFVKYNDKYYQLHDNSHRDIISFIDAYSFILQNSSKTDSIENVFDYDNTVS
TFATSLNSFYKEYNLPLMSQSESLDWLENSTRCVYPGNISKVLTNAWSTLYEIRKYQL
DFLVSNNLTSYLCNAMMLSTKEKDNADVEEQEEGEEEKALRELQFKYSYTLAQQRHIE
TAIKTLESLILSKNPNYYKAWHLLALCRSVQEDKEMSYKIVCSVLEAMNESLQNNTLL
LNDRWQFIHLKLTQLALIEEIFGTLEALETLPEVFELYATLFPDSQPKLNSMGPKYSQ
TKEYLLQMVWIFAANMYMRTKDNDEDAKAAIKEASNVESKFKNLNCNIANGYLSIIKD
EPGVALKEFETVLYYDENNLDALVGFAELIFPEELGVEETNLERYYTLSLDKKPGKRA
KLTFVNDTDRSAAYARLKFLLECAILESIEAYYSPEVWWYLSLIYEKYQDDEYKNSLL
KCIKYQELNPIRSLRYCNY"
assembly_gap 859379..859402
/estimated_length=24
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 859470..861749
/gene="Pmt6"
/locus_tag="P301_G13096"
mRNA 859470..861749
/gene="Pmt6"
/locus_tag="P301_G13096"
/product="Pmt6p"
CDS 859470..861749
/gene="Pmt6"
/locus_tag="P301_G13096"
/note="Similar to YGR199W 'Protein O-mannosyltransferase,
transfers mannose from dolichyl phosphate-D-mannose to
protein serine/threonine residues of secretory proteins;
reaction is essential for cell wall rigidity; member of a
family of mannosyltransferases'"
/codon_start=1
/product="Pmt6p"
/protein_id="EWG91147.1"
/translation="MSKAKGTGFSSIDTEDENLRERYVNQPKANASDIQDEQLDCFEQ
LEEKHRTKKNEEYTALKILRDVIGPLLLTITSFYLRFQHIDQNNYVVWDEAHFGKFGS
YYIKHEYYHDVHPPLGKMLIALSEWMAGFDGQFDFSSNNAYPENVNFKLMRQFNATFG
ALCTPVAFFTAKWMGFNYFTVYLIATMVTLEHSYIVLSKFILLDSMLLFFSMTTFACM
IKLYTLRKQQMTKKWSLWMLLTGLSIGCVCSVKWVGLFITVVVGLYTCIELFLLYCGK
ELPRIKYYKHWLIRIINLIVIPFLIYLYCFKIHFVLLYKSGTGDSTTNTLFQINLEGT
QIEAGPRDVAFGSELTIRSHGLSPNLLHSHIQVYPEGSGQRQITGYGFADSNNVWKFE
FSRSSGLELDQNGTLNGKIIPITDGVEVRLSHKNTGSNLHSHDVPSHVSRGNYEVSGY
GSQSVGDEKDDWIVEIVKQMDSPNPVYSNENSTILHPVSTFFRLRHKVLGCYLASTGL
TYPAWGFKQAEIVCKDSWSRRDKSTWWNVEDHWNHNLETAEDYVPPKSNFWTDFILTN
FAMASSNNALVPDEDKYDSLSSDAWEWPTLHKGLRMCSWAGYITRYYLMGSPFNTWIS
TVSLIIFPFIILFILYRWRRQTLYLSDDQIWQITIQGIFPFISWMTHYLPFAMMGRVT
YVHHYVPALYFAMLVFGFVLDFTLTRVHWMVKYPIYLSLFGGCIYIYNLFAPICQGMH
GDKAEYLPLQWLSTWDIAP"
gene complement(861871..864236)
/gene="Elp2"
/locus_tag="P301_G13101"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(864486..865163)
/locus_tag="P301_G13106"
mRNA complement(864486..865163)
/locus_tag="P301_G13106"
/product="hypothetical protein"
CDS complement(864486..865163)
/locus_tag="P301_G13106"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91148.1"
/translation="MSDGTLFTDLKERKLIRTIVPRGLVRSLKLDVKLADPSDAQQLY
EREFPLRKYPTFVGPHDEWTLTEAMAIDYYLIHLSSDKEAVRQLLGPEGDFKTRADIL
RWESLSNSDFLNEVCEVFFPLIGVKPYNATEFKVARENVDTIVSLYEKRLKKQQYLVC
DDHETLADLISAAAFSLGFISFFDETWRSKHPEVTRWFNRVIKSRFFEGEFESFKMCE
TEMQPIK"
gene complement(865440..866714)
/gene="Pct1"
/locus_tag="P301_G13116"
mRNA complement(865440..866714)
/gene="Pct1"
/locus_tag="P301_G13116"
/product="Pct1p"
CDS complement(865440..866714)
/gene="Pct1"
/locus_tag="P301_G13116"
/note="Similar to YGR202C Cholinephosphate
cytidylyltransferase, also known as CTP:phosphocholine
cytidylyltransferase, rate-determining enzyme of the
CDP-choline pathway for phosphatidylcholine synthesis,
inhibited by Sec14p, activated upon lipid-binding"
/codon_start=1
/product="Pct1p"
/protein_id="EWG91149.1"
/translation="MANPTTGKSSIRAKLSNSSLSNLFKKNKNKRQREETEEQDNEDK
DESKNQDENKDTQLTPRKRRRLTKEFEEKEARYTNELPKELRKYRPKGFRFNLPPTDR
PIRIYADGVFDLFHLGHMKQLEQCKKAFPNVTLIVGVPSDKITHKLKGLTVLTDKQRC
ETLTHCKWVDEVVPNAPWCVTPEFLLEHKIDYVAHDDIPYVSADSDDIYKPIKEMGKF
LTTQRTDGVSTSDIITKIIRDYDKYLMRNFARGATRQELNVSWLKKNELEFKKHINEF
RSYFKKNQTNLNNASRDLYFEVREILLKKTLGKKLYSKLIGNELKKQNQRQRKQNFLD
DPFTRKLIREASPATEFANEFTGENSTAKSPDDNGNLFSQEDDEDTNSNNTNTNSDSD
SNTNSTPPSEDDDDNDRLTLENLTQKKKQSAN"
gene 867203..867649
/locus_tag="P301_G13126"
mRNA 867203..867649
/locus_tag="P301_G13126"
/product="hypothetical protein"
CDS 867203..867649
/locus_tag="P301_G13126"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91150.1"
/translation="MDSYSITNVKYLDPTELHRWMQEGHTTTLREPFQVVDVRGSDYM
GGHIKDGWHYAYSRLKQDPEYVRELKHRLLEKQADGRGALNVIFHCMLSQQRGPSAAM
LLLRSLDTAELSRCRLWVLRGGFSRWQSVYGDDESVTAGYLPDLWR"
gene 867900..870744
/gene="Ade3"
/locus_tag="P301_G13136"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 868503..868594
/estimated_length=92
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(871030..871143)
/locus_tag="P301_G13141"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 871180..>872016
/locus_tag="P301_G13146"
mRNA 871180..>872016
/locus_tag="P301_G13146"
/product="hypothetical protein"
CDS 871180..>872016
/locus_tag="P301_G13146"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91151.1"
/translation="MCDKSKTVLDYTIEFLDKYIPEWFETGNKCPLFIFFSGPQGSGK
SFTSIQIYNHLMEKYGGEKSIGYASIDDFYLTHEDQLKLNEQFKNNKLLQGRGLPGTH
DMKLLQEVLNTIFNNNEHPDQDTVVLPKYDKSQFKGEGDRCPTGQKIKLPVDIFILEG
WFLGFNPILQGIENNDLLTGDMVDVNAKLFFYSDLLWRNPEIKSLGIVFTTDNINNVY
GWRLQQEHELISKVGKGMTDEQVHAFVDRYMPSYKLYLNDFVRSESLGSIATLTLGID
SNR"
assembly_gap 872017..872179
/estimated_length=163
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 872400..872705
/gene="Mvb12"
/locus_tag="P301_G13151"
mRNA 872400..872705
/gene="Mvb12"
/locus_tag="P301_G13151"
/product="Mvb12p"
CDS 872400..872705
/gene="Mvb12"
/locus_tag="P301_G13151"
/note="Similar to YGR206W 'ESCRT-I subunit required to
stabilize oligomers of the ESCRT-I core complex (Stp22p,
Vps28p, Srn2p), which is involved in ubiquitin-dependent
sorting of proteins into the endosome; deletion mutant is
sensitive to rapamycin and nystatin'"
/codon_start=1
/product="Mvb12p"
/protein_id="EWG91152.1"
/translation="MNNNVEELLRRIPLYNKYGKEFPQETVTRFQMPEFKLPALQPTR
DLLCPWYEECDNITKVCQLHDSSNKKFDQWYKEQYLSKKPPGIVGNTLLSPSRKDNS"
gene complement(872811..873599)
/locus_tag="P301_G13156"
mRNA complement(872811..873599)
/locus_tag="P301_G13156"
/product="hypothetical protein"
CDS complement(872811..873599)
/locus_tag="P301_G13156"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91153.1"
/translation="MSAKQQLRILVPVKRVVDFQIKPRVNKTLTGIETSGIKFSINPF
DDIAVEEAIRIKEKNKSLVESTHAVSIGSAKAQDIXXXXXXXXXXXXXXXXXXXXXXX
XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXNTGQMLAGLLNWPQATNAAKVEFL
DNGKVQVTREIDDGEEVIEASLPMVITTDLRLNTPRYVGLPKLMKAKKKPIEKLDIAK
DFPEINIEPQLKIVSMEEPKTKSPGVKLNSVDELIEKLKEVKAI"
assembly_gap 873192..873362
/estimated_length=171
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 873852..874781
/gene="Ser2"
/locus_tag="P301_G13161"
mRNA 873852..874781
/gene="Ser2"
/locus_tag="P301_G13161"
/product="Ser2p"
CDS 873852..874781
/gene="Ser2"
/locus_tag="P301_G13161"
/note="Similar to YGR208W Phosphoserine phosphatase of the
phosphoglycerate pathway, involved in serine and glycine
biosynthesis, expression is regulated by the available
nitrogen source"
/codon_start=1
/product="Ser2p"
/protein_id="EWG91154.1"
/translation="MSKFVITCIAHGENLPKETIDQIAKEITESSAKDVSINGTKKLS
ARATDIFIEVAGSIVQKDLKNKLTNVIDSHNDVDVIVSVDNEYRRAKKLFVFDMDSTL
IYQEVIELIAAYAGVEEQVHEITERAMNNELDFKESLRERVKLLQGLQVDTLYDEIKQ
KLEVTKGVPELCKFLHKKNCKLAVLSGGFIQFAGFIKNQLGLDFCKANLLEVDTDGKL
TGKTLGPIVDGQCKSETLLQLCNDYNVPVEASCMVGDGGNDLPAMATAGFGIAWNAKP
KVQKAAPCKLNTKSMTDILYILGYTDDEIYNRQ"
gene complement(874882..875197)
/gene="Trx2"
/locus_tag="P301_G13166"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(875473..876708)
/locus_tag="P301_G13171"
mRNA complement(875473..876708)
/locus_tag="P301_G13171"
/product="hypothetical protein"
CDS complement(875473..876708)
/locus_tag="P301_G13171"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91155.1"
/translation="MPRDPLIGIVGKPSSGKSTTLNSLTDAGAAVGAFPFTTIEPNQA
TGYLQVECACSRFGKEDLCKPNYGWCSKGKRHIPIKLLDVAGLVPGAHSGRGLGNKFL
DDLRHADALIHVVDVSGTTDAEGKNTRGYDPLNDIEWLQDEIRLWVEGNLKKRWGSIV
RKHTATKSSIVDTLQAQFGGYGSHAPMIQKALDRLKGLPPLEKWDDEWITQVVKSFMM
EKFPTVLALNKIDHPDADKNVSKIMLKYPDTKAVLTSAVTEVFLRKLKKQGFILYEEG
TEFVDTFEDEPDKLKPLDDKILNRIENIRDLVLYRFGSTGVVQVLQAATDILGLIPVY
TVKNIQTFTGGNGTNVFRDCFLVKRGTPVGKVARYIMGGEVTIASIETVGGVRVSEES
LVEPGKNDILGFKIAPRSA"
gene 877209..878669
/gene="Zpr1"
/locus_tag="P301_G13176"
mRNA 877209..878669
/gene="Zpr1"
/locus_tag="P301_G13176"
/product="Zpr1p"
CDS 877209..878669
/gene="Zpr1"
/locus_tag="P301_G13176"
/note="Similar to YGR211W 'Essential protein with two zinc
fingers, present in the nucleus of growing cells but
relocates to the cytoplasm in starved cells via a process
mediated by Cpr1p; binds to translation elongation factor
eEF-1 (Tef1p)'"
/codon_start=1
/product="Zpr1p"
/protein_id="EWG91156.1"
/translation="MSEQKEDLFKPVGEAAAEVEDESIAEQNKANDGVKLTGAQDAMG
HPVQEIESLCMNCGKNGTTRLLLTSIPYFREIIIMSFDCPHCGFKNCEIQPASQIQEK
GSRYVLKVECREDFNRQVIKSETATCKFVELDIEIPAKRGQLTTVEGLLSEMIDDLSQ
DQEMRKSIDEALYKKIDDFIQKVKSYINCEPNTIPITFILDDPAGNSWIEYKPGEPQH
KWSHTQYVRTDEQNVQVGIITRDQLEQRRQEQLKQLANRERNPSESVKVGSANPQFLS
DATDIENFNNEVQTFRASCPSCTQECETHMKPVNIPHFKEVIIMSTVCDHCGYKSNEV
KTGGAIPDKGRRITLYCDDAADLSRDILKSETCSMVIPELHLDIQEGTLGGRFTTLEG
LLRQVYEELESRIFTQTSDSMDEATKARWVEFFAKLKEAIAGKVKFTVIMEDPLAGSY
IQNVYAPDPDPNMTIEDYERTKEQNEDLGLSDIKVE"
gene 879008..880414
/gene="Sli1"
/locus_tag="P301_G13181"
mRNA 879008..880414
/gene="Sli1"
/locus_tag="P301_G13181"
/product="Sli1p"
CDS 879008..880414
/gene="Sli1"
/locus_tag="P301_G13181"
/note="Similar to YGR212W N-acetyltransferase, confers
resistance to the sphingolipid biosynthesis inhibitor
myriocin (ISP-1) by converting it into N-acetyl-myriocin,
co-operates with Ypk1p in mediating resistance to
myriocin"
/codon_start=1
/product="Sli1p"
/protein_id="EWG91157.1"
/translation="MNLKLSAIESYFFHRSRLNLHSCFYVGIKLNELPKKSQLIAALK
YTVIQHERLTCNVFYDELKKENFLQNILEPLKFCDLIEYHHDWDQLGETEINHIFQRY
NFSYNENKSLWKILILPNQNQMLLLTDHVLMDGMSAVHVWETFMEGLQMQQPVEIDET
IYSPSLNSSIEKIMSAPLYGDWPIPWNWHIVRQLVSRLHYWFPQTVVKNNRNLIQFAN
YSFPKDLLDDKPSDGTQKYKVKNTNHQWEFQLSPTHLNDILQECKANNTSLTSLLGAL
VCTSFEKIAAHEYTGSFLKIELPMNIRKPFERVLKLPSDDKLAVGNFIAVIEFNHKLH
QNRGIWDIASQIQRAIRSSSEDKIIDKVNEVKLLEVISSQQYIEDKISLNNGPSSTFE
VTNLGFQTFKDACNTSLPFYIVDATFNEPQGISSIFSLSVISTPGNGLHCCISYPNTL
TKVLEPHWQYMKDYLNLY"
gene complement(880480..881433)
/gene="Rta1"
/locus_tag="P301_G13191"
mRNA complement(880480..881433)
/gene="Rta1"
/locus_tag="P301_G13191"
/product="Rta1p"
CDS complement(880480..881433)
/gene="Rta1"
/locus_tag="P301_G13191"
/note="Similar to YGR213C 'Protein involved in
7-aminocholesterol resistance; has seven potential
membrane-spanning regions; expression is induced under
both low-heme and low-oxygen conditions; member of the
fungal lipid-translocating exporter (LTE) family of
protein'"
/codon_start=1
/product="Rta1p"
/protein_id="EWG91158.1"
/translation="MAKDGFELYRYTPELGASILFTVLFAVSGVAFVILLFHYSVKSK
RRVGSLMKSQPVLRYYGTVNLAGAYVPFIFGCFVECVGFAFRCKSSKDTTLLNPYIIQ
TVFLLVSPTLYAASIYMIFGRMATLLFAENLMIMPARFNTTIFVIGDVGSLLLQAIGG
AMMSKVTSASSGSHLVTAGLFIQIAFFGLFIINEVLFIFKMSKKPTSVSVRYGSWKYL
NVALLVNSFLVLIRSIVRAVEFIQGYDGEIASHEWYLYIFDGLPMFLLVLIFIVAFPL
INIFRIHEESIQAQQSARFDGTDYPDVEVTSIEEDLASKSE"
gene 882541..883754
/gene="Rps0a"
/locus_tag="P301_G13196"
mRNA join(882541..882630,883086..883754)
/gene="Rps0a"
/locus_tag="P301_G13196"
/product="Rps0ap"
CDS join(882541..882630,883086..883754)
/gene="Rps0a"
/locus_tag="P301_G13196"
/note="Similar to YGR214W 'Protein component of the small
(40S) ribosomal subunit, nearly identical to Rps0Bp;
required for maturation of 18S rRNA along with Rps0Bp;
deletion of either RPS0 gene reduces growth rate, deletion
of both genes is lethal'"
/codon_start=1
/product="Rps0ap"
/protein_id="EWG91159.1"
/translation="MSLPATFDLTPEDAQLLLAANTHLGARNVQVHQEPYVFNARPDG
VHVINVGKTWEKLVLAARIIAAIPNPEDVVAISSRTFGQRAVLKFAAHTGATPIAGRF
TPGSFTNYITRSFKEPRLVIVTDPRSDAQAIKEASYVNIPVIALTDLDSPSEFVDVAI
PCNNRGKHSIGLIWYLLAREVLRLRGALVDRTQPWSIMPDLYFYRDPEEVEQQVAEEA
TTEEAGEEEAKEEVTEEQAEATEWAEENADNVEW"
gene 884141..884473
/gene="Rsm27"
/locus_tag="P301_G13201"
mRNA 884141..884473
/gene="Rsm27"
/locus_tag="P301_G13201"
/product="Rsm27p"
CDS 884141..884473
/gene="Rsm27"
/locus_tag="P301_G13201"
/note="Similar to YGR215W Mitochondrial ribosomal protein
of the small subunit"
/codon_start=1
/product="Rsm27p"
/protein_id="EWG91160.1"
/translation="MNVPKARLLKVAELSAKIFDQNFNPSGIRTGSKILNERLKGPSV
ASYYGNPDILKFRHLKTLYPDIEFVDLEEQYRLSMVEAKKRRGKGAPKKMKKDAAATA
KGKGKKKK"
gene complement(884599..886428)
/gene="Gpi1"
/locus_tag="P301_G13206"
mRNA complement(884599..886428)
/gene="Gpi1"
/locus_tag="P301_G13206"
/product="Gpi1p"
CDS complement(884599..886428)
/gene="Gpi1"
/locus_tag="P301_G13206"
/note="Similar to YGR216C 'Membrane protein involved in
the synthesis of N-acetylglucosaminyl phosphatidylinositol
(GlcNAc-PI), the first intermediate in the synthesis of
glycosylphosphatidylinositol (GPI) anchors; human and
mouse GPI1p are functional homologs'"
/codon_start=1
/product="Gpi1p"
/protein_id="EWG91161.1"
/translation="MPNYIFWPYESLFENSAAQGPQVALAISFEKTHFVVLGVCEPQY
LEEVSIRPPYSVVATKNNGAEGWNYKVADPCNVHFRIPKLKFMQFYSSDPISLIIPEK
EVGLHSSVGETLNYSKLEQHPRYKRDNKKLSETLNIINLFPAYCKALNELYPFIQTSQ
ENLRGTMLNSVAAWCSSTCIYKMVAKIGFYLTFVICSIASLVSSLLNYSHFQLVNYSA
FVQQIDLRCQQICYFPVQYERINKKDNIQNVGSMVEKDNSNSQFSHSYMPSKFYPDYI
LLYNTIWLIINDISFGLILGAILIENRDFLVSASHRVLKFFLYDSLKTITETLANNPL
GIKLNAELANFLSELFLWVIEFSYTTFIKRLIDPKTLSSLLTLTIYMMFLVGFSFAVS
LAIDFFAILSFPIYVFYRISSKLYHCQLNIMASLFNLFCGKKRNVLRNRIDHNYFQLD
QLLLGTLLFIILVFLTPTVMAFYMSYTVLRMLTITIEIFSEAVIALINHFPLFALLLR
LKDPKRLPGGISIELKTTVSNKHTTLELQNNPIKFKSMFRPYNLLLSQMRTNYFSFAT
VRKIVRGESIMVNRNKLYYVLYSSLPSKPLSVKDLYKRLTIQA"
gene 886662..892781
/gene="Cch1"
/locus_tag="P301_G13211"
mRNA 886662..892781
/gene="Cch1"
/locus_tag="P301_G13211"
/product="Cch1p"
CDS 886662..892781
/gene="Cch1"
/locus_tag="P301_G13211"
/note="Similar to YGR217W 'Voltage-gated high-affinity
calcium channel involved in calcium influx in response to
some environmental stresses as well as exposure to mating
pheromones; interacts and co-localizes with Mid1p,
suggesting Cch1p and Mid1p function together'"
/codon_start=1
/product="Cch1p"
/protein_id="EWG91162.1"
/translation="MQGRKRTLTEPFEPNTNPFGDNAAVMTENVEDNSETDGNRLESK
PQALVPPALNIVPPESSIHSTEEKKGDEYNGNDKDSSLISNIFRTRVGRSSHENLSRP
KLSLKTASFGAAESSRRNVSPSTKSAKSSSQYIDLNDERLRRRSFSSYSRSSSRRVSN
SPSSTDRPPRSAKVLSLIAADDMDDFEDLQKGFKSAIDEEGLTWLPQLKSEKSRPVSD
VGEDRGEGEQESIPDVHTPNVGASATPGSIHLTPEPAQNGSVSEGLEGSINNSRKKPS
PKFFHHLSPQKEDKDQTEVIEYAEDILDFETLQRKLESRPFVLYGHSLGVFSPTNPLR
IKIARFLLHRRYSLLYNTLLTFYAILLAIRTYNPHNVVFLYRFSNWTDYFIFILSACF
TGNDIAKIIAFGFWDDSEMFKAYGREYKSILQRSGIMKLYIYLREKYGRKLIDFIIPF
RIISPGEETKYQRSSLSTSLTKPYGAKENQRPFGTPRAFARSSWNRIDLVSSVSFWLG
MFLSIKSYDTKTGIRIFKPLAILRILRLVNVDTGMPSILRGLKYGIPQLVNVSSMLVY
FWIFFGILGVQIFQGSFRRQCVWFNPEDPTDTYQYDMQFCGGYLDPVTKRKQNYIYED
GSEGSVSKGFLCPQYSKCVSNANPYNGRISFDNIVNSMELVFVIMSANTFTDLMYYTM
DSDEMAACLFFIVCIFVLTIWLLNLLIAVLVSSFEIANEEYKKKKFIYGSRKTGYVAR
IVTGYWKYFKLKANQTKFPNWSQKGLAIYSHVEFIFVILIICDIGMRASVKVSTSANY
NNILLKTDRGISIVLFIESLARLVLYLPNMWKFLTKPSYVYDFIISIITLVISCLAVE
GVLGHMYAWLSIFHISRFYRVIISFKLTKKLWKQILSNGVMIWNLSSFYFFFTFLVAI
IMAVYFEGVIPPEEMADQPFGMYSLPNSFLSLFIIGSTENWTDILYALQKHSPNISSA
FFCSVFFIIWFLLSNSVILNIFIALISESMEVKEEEKRPQQIKHYLKFVYPQKIQEYT
HASLVARIRKKFFGGHRNEDTRDFKQFLMRGTAIMNIAQNMGELADEFKEPPSENLFK
KGLSKLTIGVPSLKRLRMFANNPFYKNSDVVFTETNDINGRTYILELNEYEDEKLDYL
KKYPLFNYSYYFFSPQHRFRRFCQRLVPPSTGKRTDGSRFFEDSTDLYNKRSYFHHIE
RDVFVFIFALATILLIVCSCYVTPLYRMHHKMGTWNWSSALDCAFIGAFSIEFIVKTV
ADGFIYSPNAYLRNPWNFIDFCVLISMXXXXXXXXXXXXXXXRIFKGLTALRALRCLT
ISNTARQTFNLVMFDGLNKIFEAGLISLSLLFPFTVWGLSIFKGRLGTCNDGSLGRAD
CYNEYSNSVFQWDIMSPRVYQQPYLHLDSFASAFNSLYQIISLEGWVDLLENMMNSSG
IGTPATVMGSAGNALFLVLFNFLSMVFILNLFVSFIVNNQARTTGSAYFTIEEKAWLE
SQKLLSQAKPKAIPNLIELSRVRQFFYQLAVEKKNFYYASFLQVVLYLHIIMLLSRSY
NPGNLIGYQGVYFMFSTSVFLIQEALHMCGEGPRLYFRQKWNSIRLSIIIIAFIVNAV
AFHVPASHYWFHNIKGVFLLVIFLFIIPQNDTLTELLETAMASLPPILSLTYTWGVLF
LVYAIALNQIFGLTRLGSNTTDNINFRTVIKSMIVLFRCSFGEGWNYIMADLTVSEPY
CSSDDNSTYTDCGSETYAYLLLMSWNIISMYIFVNMFVSLIIGNFSYVYRSGGSRSGI
NRSEIKKYIEAWSKFDTDGTGELELSYLPRIMHSFDGPLSFKIWEGRLTIKSLVENYM
EVNPDDPYDVKIDLIGLNKELNTIDKAKIIQRKLQYRRFVQSIHYTNAYNGCIRFSDL
LLQIPLYTAYSARECLGIDQYVHHLYILGKVDKYLENQRNFDVLEMVVTRWKFHCRMK
RTIEPEWDVKDPTVSSHISNINVNLEPAPGILEREPIATPRMDYGVNNFMWSPRMNQD
STMEPPEEPIDNNDDSANDLIDR"
assembly_gap 890530..890571
/estimated_length=42
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(892919..892989)
/locus_tag="P301_G13216"
tRNA complement(892919..892989)
/locus_tag="P301_G13216"
/product="tRNA-Gly"
/note="TG(GCC)G2"
assembly_gap 893276..893710
/estimated_length=435
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 894132..897386
/gene="Crm1"
/locus_tag="P301_G13221"
mRNA 894132..897386
/gene="Crm1"
/locus_tag="P301_G13221"
/product="Crm1p"
CDS 894132..897386
/gene="Crm1"
/locus_tag="P301_G13221"
/note="Similar to YGR218W 'Major karyopherin, involved in
export of proteins, RNAs, and ribosomal subunits from the
nucleus; exportin'"
/codon_start=1
/product="Crm1p"
/protein_id="EWG91163.1"
/translation="MEGILDFSNDLDIALLDQVVSTFYQGSGVQQKQAQEILTKFQDN
PDAWQKADQILQFSTNPQSKFIALSILDKLITRKWKLLPNDHRIGIRNFVVGMIISMC
QDDEVFKTQKNLINKSDLTLVQILKQEWPQNWPEFIPELIGSSSSSVNVCENNMIVLK
LLSEEVFDFSAEQMTQAKALHLKNSMSKEFEQIFKLCFQVLEQGSSSSLIVATLESLL
RYLHWIPYRYIYETNILELLSTKFMTSPDTRAITLKCLTEVSNLKIPQDNHLIKRQTV
LFFQNTLQQIATSVMPVTADLKATYANANGNDQSFLQDLAMFLTTYLARNRALLESDE
SLRELLLNAHQYLIQLSKIEERELFKTTLDYWHNLVADLFYEVQRLPATEMSPLIQLS
VGSQAISTGSGALNPEYMKRFPLKKHIYEEICSQLRLVIIENMVRPEEVLVVENDEGE
IVREFVKESDTIQLYKSEREVLVYLTHLNVIDTEEIMISKLARQIDGSEWSWHNINTL
SWAIGSISGTMSEDTEKRFVVTVIKDLLDLTVKKRGKDNKAVVASDIMYVVGQYPRFL
KAHWNFLRTVILKLFEFMHETHEGVQDMACDTFIKIVQKCKYHFVIQQPRESEPFIQT
IIRDIQKTTADLQPQQVHTFYKACGIIISEERSVAERNRLLSDLMQLPNMAWDTIVEQ
STANPTLLLDSETVKIIANIIKTNVAVCTSMGADFYPQLGHIYYNMLQLYRAVSSMIS
AQVAAEGLIATKTPKVRALRTIKKEILKLVETYISKARNLDDVVKVLVEPLLNAVLED
YMNNVPDARDAEVLNCMTTVVEKVGHMIPQGVILILQSVFECTLDMINKDFTEYPEHR
VEFYKLLKVINEKSFAAFLELPPAAFKLFVDAICWAFKHNNRDVEVNGLQIALDLVKN
IERMGNVPFANEFHKNYFFIFVSETFFVLTDSDHKSGFSKQALLLMKLISLVYDNKIS
VPLYQEAEVPQGTSNQVYLSQYLANMLSNAFPHLTSEQIASFLSALTKQYKDLVVFKG
TLRDFLVQIKEVGGDPTDYLFAEDKENALMEQNRLEREKAAKIGGLLKPSELDD"
gene complement(897665..898474)
/gene="Mrpl9"
/locus_tag="P301_G13231"
mRNA complement(897665..898474)
/gene="Mrpl9"
/locus_tag="P301_G13231"
/product="Mrpl9p"
CDS complement(897665..898474)
/gene="Mrpl9"
/locus_tag="P301_G13231"
/note="Similar to YGR220C Mitochondrial ribosomal protein
of the large subunit"
/codon_start=1
/product="Mrpl9p"
/protein_id="EWG91164.1"
/translation="MSKFLQGSIFSISKLHVRYSSTRPFLVAPSIANSITTEAPAINH
SPELANARKWLPKRCGLITRKKGMMPYFDKSTGERSAATILEVNNVEVIMHRTSEVNG
YFACQVGYGSRHLSKVSRQMLGHFASKVVNPKEHVAEFRVKDEKGLIPPGTLLKPSFF
KEGQYVDVRSVSKGKGFTGVMKRYGFKGLRASHGTSIMHRHGGSYGQNQDPGRVLPGR
KMPGHMGNEHVTIQNVKVLKVDDENNVIWVKGSVAGPKNSFVKIQDAIKKT"
gene complement(898715..900583)
/gene="Tos2"
/locus_tag="P301_G13241"
mRNA complement(898715..900583)
/gene="Tos2"
/locus_tag="P301_G13241"
/product="Tos2p"
CDS complement(898715..900583)
/gene="Tos2"
/locus_tag="P301_G13241"
/note="Similar to YGR221C 'Protein involved in
localization of Cdc24p to the site of bud growth; may act
as a membrane anchor; localizes to the bud neck and bud
tip; potentially phosphorylated by Cdc28p'"
/codon_start=1
/product="Tos2p"
/protein_id="EWG91165.1"
/translation="MFSHYRYKENSCQKREAIPDKSRVSLTFLQKRTDSSNVTVAVAV
AVPIGAIIIVLSVVLIVVYRRCKKEPSMQDLDPNFEGDLYYLPKMDSSMNSANSDSNA
TEKRFIYGGYDDFLQPSIENSQSFKDYVRRINEHAPSAYNIASLASQNNSKLSVPSKH
IDLSNKISFESLENSELIVSPQHSNTGQDCDQRCDSTSNPDVNEKSSHNNDNRLKSNY
TSRSGLEPQCSREEEENIDRIRSIYNIYFEKSNSTIRSSVTSSIRRDSKLNIATRKSV
NMSSQDNPNDTTLIEQSHFGSTTVQEIDSSSSANEEYEDATDYLQVPAPQENKNIASS
VYSEVATREKVIPESSLSLTFPPPNGLSTRITSSIYSDTVAKGHIHSAKVPVRALSEG
SGQSNLTSAQQYPTYFIDHCNQSNDDNYYYNYPLPLEHPQNYENIGDLPTPTQFIYST
SSHSLTSFKGRPKPPKTLKHVPTARLNGTALNPMDHPEMFYSSPTKIPSTSLTKQFCT
PLPYQLRQSVVMTNPSELSMKPRYKPAGSLRNLIKAQYLPGNSSTTTSSSLSQPPSTL
SNAINFRVSGLLDDTDILQPPSVGETLPFKASTEDLRKQLGTSHNYEITPYENVHV"
gene complement(901050..901263)
/locus_tag="P301_G13246"
ncRNA complement(901050..901263)
/ncRNA_class="snoRNA"
/locus_tag="P301_G13246"
/product="SNR7-L"
gene complement(901085..901263)
/locus_tag="P301_G13251"
ncRNA complement(901085..901263)
/ncRNA_class="snoRNA"
/locus_tag="P301_G13251"
/product="SNR7-S"
gene 901514..902395
/gene="Pet54"
/locus_tag="P301_G13256"
mRNA 901514..902395
/gene="Pet54"
/locus_tag="P301_G13256"
/product="Pet54p"
CDS 901514..902395
/gene="Pet54"
/locus_tag="P301_G13256"
/note="Similar to YGR222W 'Mitochondrial inner membrane
protein; binds to the 5' UTR of the COX3 mRNA to activate
its translation together with Pet122p and Pet494p; also
binds to the COX1 Group I intron AI5 beta to facilitate
exon ligation during splicing'"
/codon_start=1
/product="Pet54p"
/protein_id="EWG91166.1"
/translation="MKASSKAIKLVLDHLKSTGRVLGSVESGNSATISEKTASVNKQQ
QLQEKKPSVLQYRSYNPYLVKEDFLSILPENLYKKRGQFTNELDFQLMKVRDPKYFQF
KDQYYLFFNDYNSLTEYIKLTKHSRINKIRVKMTPLAQPLPTLLTKLQRYSKNLYNAF
RSSEQYFEGLNEKVDVSGEFTTNQLRSILDSVEEIENKSVLVWNIPTKLRSHDILNYF
WFYNIRSSFKIYWDDEMKRNLRFISFENSHDAYRFKRNYHGLLAKELLILSEKGDAAD
YSLEMDDSKILIEHLSE"
gene complement(902460..903806)
/gene="Hsv2"
/locus_tag="P301_G13261"
mRNA complement(902460..903806)
/gene="Hsv2"
/locus_tag="P301_G13261"
/product="Hsv2p"
CDS complement(902460..903806)
/gene="Hsv2"
/locus_tag="P301_G13261"
/note="Similar to YGR223C 'Phosphatidylinositol
3,5-bisphosphate-binding protein, plays a role in
micronucleophagy; predicted to fold as a seven-bladed
beta-propeller; displays punctate cytoplasmic
localization'"
/codon_start=1
/product="Hsv2p"
/protein_id="EWG91167.1"
/translation="MDVRRPIREAVNNRRKPKFLSVSFNQDDSCFSVALENGFRIFNT
DPLTSKLSKTFKESGTNQSRGTGIGYTRMLYRTNYIALVGGGKRPRHALNKLIIWDDL
LQKETITLKFMSSIKDVFLSRIHIVVVLENTIEIFQFQTNPQRICPILDIPPNGSVDY
VVCSSKHLQSQASQSQSKILEIIAFPSNKCVGQIQVADLSQIKYNSQNPKESALLPTS
IIKAHKNPIKLVRLNRQGTMVATCSVQGTLIRIFSTHNGTLIKEFRRGVDKADIYEMS
FSPNGSKLAVLSNKQTLHIFQIFETTNTETNTPDHSRANGSSHPLKNYIPKGLWRPKY
LDSVWSICNAHLKNPIFDAHRNDNSGDVTHDNEFYKDRCRIGWCQDSNNREQDDSLVL
VWQNSGIWEKFVILEKEQQDSSKTHYSLNESLRNEDTKSAGEPTRWELVRESWREL"
gene 904397..906237
/gene="Azr1"
/locus_tag="P301_G13271"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 906738..908612
/gene="Ama1"
/locus_tag="P301_G13276"
mRNA join(906738..907920,908014..908612)
/gene="Ama1"
/locus_tag="P301_G13276"
/product="Ama1p"
CDS join(906738..907920,908014..908612)
/gene="Ama1"
/locus_tag="P301_G13276"
/note="Similar to YGR225W 'Activator of meiotic anaphase
promoting complex (APC/C); Cdc20p family member; required
for initiation of spore wall assembly; required for Clb1p
degradation during meiosis'"
/codon_start=1
/product="Ama1p"
/protein_id="EWG91168.1"
/translation="MATRHLYYRYNSKSSNKNINSSGNSTEVDRFIPKSVSRNAYKSI
PMLNGFDISYSELCEKSPSPERLSSPEFFNELRNTGHYESISTTNEFSMSSISSSSES
QVTRSGSARASRNDYSKLTKEQKDHRKNIAHSLGFQLPDRVFTFETTSAEILEKNKAI
KNCFGPGSCAEIRSTFDFSTLSPDVARYYIANSNARSASPQRQIQRPAKRVKSHIPYR
VLDAPCLRNDFYSNLISWSRTTNNVLVGLGCSVYIWSEKEGAVSILDHQYLSEKRDLV
TCVSFCPYNTYFIVGTKFGRILLYDQKEFFHSSNTNEKEPVFVFQTESFKGICCLEWF
KPGEICKFYVGEENGNVSLFEIKSLHFSIKNWSKRQKLEDENLIGLKLHSTYQAQAQQ
VCGISLNEHANLLAVGGNDNSCSLWDISDLDKPIKKFVLPHKAAVKAIAFCPWSKSLL
ATGGGSKDRCIKFWHTSTGTLLDEIYTSGQVTSLIWSLRHKQIVATFGFGDTKNPVLI
TLYSYPKLSKLLEVRSPNPLRVLSAVISPSSMAICVATNDETIRFYELWNDKEEIINE
IQESGIYGSNIIEYMEGIETTHNKRIR"
gene 909011..910588
/gene="Die2"
/locus_tag="P301_G13286"
mRNA 909011..910588
/gene="Die2"
/locus_tag="P301_G13286"
/product="Die2p"
CDS 909011..910588
/gene="Die2"
/locus_tag="P301_G13286"
/note="Similar to YGR227W
Dolichyl-phosphoglucose-dependent alpha-1,2
glucosyltransferase of the ER, functions in the pathway
that synthesizes the dolichol-linked oligosaccharide
precursor for N-linked protein glycosylation, has a role
in regulation of ITR1 and INO1"
/codon_start=1
/product="Die2p"
/protein_id="EWG91169.1"
/translation="MDAKKNTGEANNDVLEEEAAIQLIAPGIARNLTQEVITGIFCNV
VIYPLLLIYFVLTFRYVTTNIVPYEFIDEKFHVGQTLTYLKGKWTQWDPKITTPPGIY
ILGLINYYCIKPIFKSWSTLTILRLVNLLGGIIVFPILVLRPIFLFNALGFWPVSLMS
FPLMTTYYYLFYTDVWSTILILQSLSCVLTLPFGPVKSIWLSAFFAGVSCLFRQTNII
WTGFIMILAVERPAILQKQFNTHTFNNYLKLFIHAIDDFSNLVLPYMINFVLFFIYLI
WNRSITLGDKSSHSAGLHIVQIFYCFTFITVFSLPIWISRNFMKLYKLRIKRKPVQTF
FEFIGIMLTIRYFTKVHPFLLADNRHYTFYLFRRLIGNKSRLIKYFFMTPIYHFSTFA
YLEVMRPNQLTFHPITPLPIKEPVHLPIQLTHVSWTALITCTMVTIVPSPLFEPRYYI
LPYFFWRIFITCSCEPLIKDLKPAKEGENPITISSTKRLFMEFLWFMLFNVVTLVIFS
KVSFPWTTEPYLQRIIW"
assembly_gap 910870..910931
/estimated_length=62
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(910977..912494)
/gene="Smi1"
/locus_tag="P301_G13296"
mRNA complement(910977..912494)
/gene="Smi1"
/locus_tag="P301_G13296"
/product="Smi1p"
CDS complement(910977..912494)
/gene="Smi1"
/locus_tag="P301_G13296"
/note="Similar to YGR229C 'Protein involved in the
regulation of cell wall synthesis; proposed to be involved
in coordinating cell cycle progression with cell wall
integrity'"
/codon_start=1
/product="Smi1p"
/protein_id="EWG91170.1"
/translation="MDLFKRKVKEWVYSLSTDDHYAEYNPDETPTFNMGKRLNSNNGQ
VNPSQMHLNSVDEEMSMGFQNGVPSNEDINIDEFTSTESNDGVSETLLAWRHIDFWTS
EHNPDLNATLSDPCTQNDITHAEEDLEVSFPNPVKASFKIHDGQEDLESMTGTSGLFY
GFQLMTLDQVVAMTQAWRNVAKNLNKRSQQGLSHVTSTGSSSSMERLNGNKFKLPNIP
DQKSIPPNAVQPVYAHPAWIPLITDNAGNHIGVDLAPGPNGKYAQIITFGRDFDTKFV
IAENWGEFLLSFANDLEAGNWYLVDDNDDYFSGDGELVFRDKKSNGPIQDYFEVLKRR
TWIKYQENLRSQQQKSQPDTSLQEQKYMPASQKKVAAEQPSTLNAESIKGEDSGSADV
QSVQDHESVKIVKTEPSEAETTTVNTESLGQAEHEIKADNVDTKQESERKEDEKQPKV
EEKEHVENEHVTESAKKDDDVNKQTEEMNKKEENEIRSDDAKVEEAREEFENIAL"
gene 913488..913900
/gene="Bns1"
/locus_tag="P301_G13301"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(914141..915073)
/gene="Phb2"
/locus_tag="P301_G13306"
mRNA complement(914141..915073)
/gene="Phb2"
/locus_tag="P301_G13306"
/product="Phb2p"
CDS complement(914141..915073)
/gene="Phb2"
/locus_tag="P301_G13306"
/note="Similar to YGR231C 'Subunit of the prohibitin
complex (Phb1p-Phb2p), a 1.2 MDa ring-shaped inner
mitochondrial membrane chaperone that stabilizes newly
synthesized proteins; determinant of replicative life
span; involved in mitochondrial segregation'"
/codon_start=1
/product="Phb2p"
/protein_id="EWG91171.1"
/translation="MNRSPGEFQRYAKAFQKQLSKVQQTGGRGQVPSPRGAFAGLGGL
LLLGGGALFINNALFNVDGGHRAIVYSRIHGVSSRIFNEGTHFIFPWLDTPIIYDVRA
KPRNVASLTGTKDLQMVNITCRVLSRPDVVQLPTIYRTLGQDYDERVLPSIVNEVLKA
VVAQFNASQLITQREKVSRLIRENLVRRASKFNILLDDVSITYMTFSPEFTNAVEAKQ
IAQQDAQRAAFVVDKARQEKQGMVVRAQGEAKSAELIGEAIKKSRDYVELKRLDTARD
IAKILASSPNRVILDNEALLLNTVVDARIDGRGK"
gene 915554..916243
/gene="Nas6"
/locus_tag="P301_G13311"
mRNA 915554..916243
/gene="Nas6"
/locus_tag="P301_G13311"
/product="Nas6p"
CDS 915554..916243
/gene="Nas6"
/locus_tag="P301_G13311"
/note="Similar to YGR232W 'Proteasome-interacting protein
involved in the assembly of the base subcomplex of the 19S
proteasomal regulatory particle (RP); ortholog of human
oncoprotein gankyrin, which interacts with the Rb tumor
suppressor and CDK4/6'"
/codon_start=1
/product="Nas6p"
/protein_id="EWG91172.1"
/translation="MSNNYPLHQACMENEFFKVQELLHSKPSLLLQKDQDGRIPLHWS
VSFQAHEITSFLLSKMENVNLDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLN
KITNQGVTCLHLAVGKKWFEVSQFLIENGASVGIKDKFNQIPLHRAASVGSLKLIELL
CGLGKSPVNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAKAEDVALN
EQVKKFFLNNV"
gene complement(916271..919852)
/gene="Pho81"
/locus_tag="P301_G13316"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 919101..919176
/estimated_length=76
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 921541..922740
/gene="Yhb1"
/locus_tag="P301_G13331"
mRNA 921541..922740
/gene="Yhb1"
/locus_tag="P301_G13331"
/product="Yhb1p"
CDS 921541..922740
/gene="Yhb1"
/locus_tag="P301_G13331"
/note="Similar to YGR234W 'Nitric oxide oxidoreductase,
flavohemoglobin involved in nitric oxide detoxification;
plays a role in the oxidative and nitrosative stress
responses'"
/codon_start=1
/product="Yhb1p"
/protein_id="EWG91173.1"
/translation="MLAEKTRSIIKATVPVLEQQGTVITRTFYKNMLTEHTELLNIFN
RTNQKVGAQPNALATTVLAAAKNIDDLSVLMDHVKQIGHKHRALQIKPEHYPIVGEYL
LKAIKEVLGDAATPEIINAWGEAYQAIADIFITVEKKMYEEALWPGWKPFEITAKEYV
ASDIVEFTVKPKFGSGIELESLPITPGQYITVNTHPIRQENQYDALRHYSLCSASTKN
GLRFAVKMEAARENFPAGLVSEYLHKDAKVGDEIKLSAPAGDFAINKELIHQNEVPLV
LLSSGVGVTPLLAMLEEQVKCNPNRPIYWIQSSYDEKTQAFKKHVDELLAECANVDKI
IVHTDTEPLIDAAFLKEKSPAHADVYTCGSVAFMQAMIGHLKELEHRDDMIHYEPFGP
KMSTVQV"
gene complement(922997..923698)
/locus_tag="P301_G13336"
mRNA complement(922997..923698)
/locus_tag="P301_G13336"
/product="hypothetical protein"
CDS complement(922997..923698)
/locus_tag="P301_G13336"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91174.1"
/translation="MTKDFYRQLDPVEEKIVPPENAIVISSEAKEATVNEKEAKQGVL
SQRVMKYIGENELVDGISVRDPDYLKRFFNERRKQFSAKWDKVTNKIDDIAGRYYARE
ESFTSTIASLHTDPNERLIPGLLSILVASMTGSVLARRRTWLLRATMPIILGSCCFAY
AMPTTFRNTMGLIHNLEMNTFPHFTERQDRVWKETKRLSTASVQYYYDAKKWLNKDVE
KTGNAIKNWTGVNVK"
gene complement(924167..924454)
/gene="Spg1"
/locus_tag="P301_G13341"
mRNA complement(924167..924454)
/gene="Spg1"
/locus_tag="P301_G13341"
/product="Spg1p"
CDS complement(924167..924454)
/gene="Spg1"
/locus_tag="P301_G13341"
/note="Similar to YGR236C 'Protein required for survival
at high temperature during stationary phase; not required
for growth on nonfermentable carbon sources; the
authentic, non-tagged protein is detected in highly
purified mitochondria in high-throughput studies'"
/codon_start=1
/product="Spg1p"
/protein_id="EWG91175.1"
/translation="MKLDSGIYSEAQRVVRTPKFRYIMLGLVGAAAVPTAYMRRGYTV
PAHSLDNINGVDTTKASVMGTEQRAAMTKGKSLQEMMDDDEVTYLMFSSIM"
gene complement(924925..927251)
/locus_tag="P301_G13346"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(927634..930283)
/gene="Kel2"
/locus_tag="P301_G13351"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 927709..927730
/estimated_length=22
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(930782..931648)
/gene="Pex21"
/locus_tag="P301_G13366"
mRNA complement(930782..931648)
/gene="Pex21"
/locus_tag="P301_G13366"
/product="Pex21p"
CDS complement(930782..931648)
/gene="Pex21"
/locus_tag="P301_G13366"
/note="Similar to YGR239C 'Peroxin required for targeting
of peroxisomal matrix proteins containing PTS2; interacts
with Pex7p; partially redundant with Pex18p'"
/codon_start=1
/product="Pex21p"
/protein_id="EWG91176.1"
/translation="MPSVCHTSPIEKIIQQGHRIQNDSLIPSKRTKLAHTELTAHYAT
EDSHVEKHFLHNGSNFDGIDNVRYQNQPSPLTFITPNNTVDSSDWVPQFSSMKIDDSL
EFSSEYKRLYSNYESQQRLNSSRQHLPFKNCMIRKTSCTYPPQKTLRQQRQGNRDNPT
DAFQFDAEFQVLEREIQKERYEPITRRDEKWFDQDQSELQRIATDIVKCCTPPPSSAS
SSSTLSSSVESKLSESKFIQLMRNISSGDVTLKKNADGNSASELFSSNNGELVGNRHI
FVKDEIHKDILD"
gene complement(932366..935329)
/gene="Pfk1"
/locus_tag="P301_G13376"
mRNA complement(932366..935329)
/gene="Pfk1"
/locus_tag="P301_G13376"
/product="Pfk1p"
CDS complement(932366..935329)
/gene="Pfk1"
/locus_tag="P301_G13376"
/note="Similar to YGR240C Alpha subunit of heterooctameric
phosphofructokinase involved in glycolysis, indispensable
for anaerobic growth, activated by
fructose-2,6-bisphosphate and AMP, mutation inhibits
glucose induction of cell cycle-related genes"
/codon_start=1
/product="Pfk1p"
/protein_id="EWG91177.1"
/translation="MQSQDSCYGVAFRSIITNDEALFKKTIHFYHTLGFATVKDFNKF
KHGENSLLSSGTSQDSLREVWLESFKLSEVDASGFRIPQQEATNKAQSQGALLKIRLV
MSAPIDETFDTNETATITYFSTDLNKIVEKFPKQAEKLSDTLVFLKDPMGNNITFSGL
ANATDSAPTSKDAFLEATSEDEIISRASSDASDLLRQTLGSSQKKKKIAVMTSGGDSP
GMNAAVRAVVRTGIHFGCDVFAVYEGYEGLLRGGKYLKKMAWEDVRGWLSEGGTLIGT
ARSMEFRKREGRRQAAGNLISQGIDALVVCGGDGSLTGADLFRHEWPSLVDELVAEGR
FTKEEVAPYKNLSIVGLVGSIDNDMSGTDSTIGAYSALERICEMVDYIDATAKSHSRA
FVVEVMGRHCGWLALMAGIATGADYIFIPERAVPHGKWQDELKEVCQRHRSKGRRNNT
IIVAEGALDDQLNPVTANDVKDALIELGLDTKVTILGHVQRGGTAVAHDRWLATLQGV
DAVKAVLEFTPETPSPLIGILENKIIRMPLVESVKLTKSVATAIENKDFDKAISLRDT
EFIELYENFLSTTVKDDGSELLPVSDRLNIGIVHVGAPSAALNAATRAATLYCLSHGH
KPYAIMNGFSGLIQTGEVKELSWIDVENWHNLGGSEIGTNRSVASEDLGTIAYYFQKN
KLDGLIILGGFEGFRSLKQLRDGRTQHPIFNIPMCLIPATVSNNVPGTEYSLGVDTCL
NALVNYTDDIKQSASATRRRVFVCEVQGGHSGYIASFTGLITGAVSVYTPEKKIDLAS
IREDITLLKENFRHDKGENRNGKLLVRNEQASSVYSTQLLADIISEASKGKFGVRTAI
PGHVQQGGVPSSKDRVTASRFAVKCIKFIEQWNKKNEASPNTDAKVLRFKFDTHGEKV
PTVEHEDDSAAVICVNGSHVSFKPIANLWENETNVELRKGFEVHWAEYNKIGDILSGR
LKLRAEVAALAAENK"
assembly_gap 935656..935688
/estimated_length=33
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(936164..936364)
/locus_tag="P301_G13386"
mRNA complement(936164..936364)
/locus_tag="P301_G13386"
/product="hypothetical protein"
CDS complement(936164..936364)
/locus_tag="P301_G13386"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91178.1"
/translation="MILSKHVNFTYHTYNLIYNFYPDLTNFGMPGTIYGLLSSLKTME
KHIEIPQSIHHYSPFYQLPLIF"
gene complement(936462..938168)
/gene="Yap1802"
/locus_tag="P301_G13391"
mRNA complement(936462..938168)
/gene="Yap1802"
/locus_tag="P301_G13391"
/product="Yap1802p"
CDS complement(936462..938168)
/gene="Yap1802"
/locus_tag="P301_G13391"
/note="Similar to YGR241C 'Protein involved in clathrin
cage assembly; binds Pan1p and clathrin; homologous to
Yap1801p, member of the AP180 protein family'"
/codon_start=1
/product="Yap1802p"
/protein_id="EWG91179.1"
/translation="MSSLYTKLVKGATKIKMAPPKQKYVDPILSGTSSARGLQEITHA
LDIRLSDTAWTIVYKALIVLHLMIQQGEKDVTLRHYSHNLDVFQLRKISHTTKWSSND
MRALQRYDEYLKTRCEEYGRLGMDHLRDNYSSLKLGSKNQLSMDEELDHVESLEIQIN
ALIRNKYSVSDLENHLLLYAFQLLVQDLLGLYNALNEGVITLLESFFELSIEHAKRTL
DLYKDFVDMTEYVVRYLKIGKAVGLKIPVIKHITTKLINSLEEHLREETKRQRGEPSE
PQQDRKPSTAISSTSSHNNNSNDKNKSIAQKKLEQIREQKRLLEQQLQNQQLLISPTV
PQDAYNPFGSQQQELNNDTFSFEPTQPQMTAQVPQPTANPFLIPQQQQQALQLTSAST
MPQPSEIQITPNSNNQQTGMYASNLQYTPNFTGSGFGGYTTTENNAMMTGTLDPTKTG
SNNPFSLENIAREQQQQNFQNSPNPFTLQQAQTTPILAHSQTGNPFQAQNVVTSPMGT
YMTSPVAGQLQYASTGAQQQPQMMQGQQTGYVMVPTAFVPINQQQQQQQHQQENPNLI
DI"
gene 938921..939361
/gene="Fmp43"
/locus_tag="P301_G13401"
mRNA 938921..939361
/gene="Fmp43"
/locus_tag="P301_G13401"
/product="Fmp43p"
CDS 938921..939361
/gene="Fmp43"
/locus_tag="P301_G13401"
/note="Similar to YGR243W 'Putative protein of unknown
function; expression regulated by osmotic and alkaline
stresses; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies'"
/codon_start=1
/product="Fmp43p"
/protein_id="EWG91180.1"
/translation="MSASAFNFAFRRFWNSETGPKTVHFWAPTLKWGLVFAGLNDIKR
PVEKVSGAQNLSLLATALIWTRWSFVIKPKNYLLASVNFFLGCTAGYHLTRIANFRIR
NGDSFKQVIHYIIKGETPAAVAAKQTASTSMNKGVIGTNPPITH"
gene complement(939625..940908)
/gene="Lsc2"
/locus_tag="P301_G13406"
mRNA complement(939625..940908)
/gene="Lsc2"
/locus_tag="P301_G13406"
/product="Lsc2p"
CDS complement(939625..940908)
/gene="Lsc2"
/locus_tag="P301_G13406"
/note="Similar to YGR244C Beta subunit of succinyl-CoA
ligase, which is a mitochondrial enzyme of the TCA cycle
that catalyzes the nucleotide-dependent conversion of
succinyl-CoA to succinate"
/codon_start=1
/product="Lsc2p"
/protein_id="EWG91181.1"
/translation="MYSRKSLSLISKCGQLSRLNAQAALQARRHLSIHEYRSAQLLRE
YGIGTPEGFPAFTPEEAFEAAKKLNTNKLVIKAQALTGGRGKGHFDTGYKSGVHMIES
PQQAEDVAKEMLNHNLITKQTGIAGKPVSAVYIVKRVDTKHEAYLSILMDRQTKKPMI
IASSQGGMNIEEVAERTPDAIKKFSIETSKGLSPQMAKDVAKSLGFSPDAQDEAAKAV
SNLYKIFMERDATQVEINPLSEIEHDPTHKIMCTDAKFGFDDNASFRQEKIYSWRDLS
QEDPDEVKAKKYDLNFVKLKGNIGCLVNGAGLAMATMDVIKLNGGDPANFLDCGGGAT
PETIKQGFELILSNKNVDAIFVNIFGGIVRCDYVALGLVEAARQLEVRVPIVARLQGT
KVEEGRDIINKSGVKIYSFDELDPAAKKVVELTQN"
gene complement(941354..943657)
/gene="Sda1"
/locus_tag="P301_G13411"
mRNA complement(941354..943657)
/gene="Sda1"
/locus_tag="P301_G13411"
/product="Sda1p"
CDS complement(941354..943657)
/gene="Sda1"
/locus_tag="P301_G13411"
/note="Similar to YGR245C Highly conserved nuclear protein
required for actin cytoskeleton organization and passage
through Start, plays a critical role in G1 events, binds
Nap1p, also involved in 60S ribosome biogenesis"
/codon_start=1
/product="Sda1p"
/protein_id="EWG91182.1"
/translation="MGRRSRAAMLPTNIILLQNLVKRDPESYQEEFLQQYAHYESLRD
IFMLNGLAGGDSAAATNGLDVGNGSSTMAGTNGTTMSTSTSQLIELVGFVSQVCSCFP
RETANFPSELKQLLLEHHKSLPFELKEKILSCLTMLRNKDVITAEELIQSLFPLLVAY
SSHGNSLGVNSHAKELRKIIYTNLISLLKSCNTNGKNQKLNKSTQAVCFNLLDQPDSQ
GIWATKLTRELWRRGIWDDSRTVEIMTQAALHQDVKIVMSGVMFFLDADREREENFEE
NSEDEDGFDLDALRHKMQVNKKTGRRGKKLENAIKTVKKKKKNGPGAPQGYLNFSAIH
LLRDPQGFAEKLFKEHLSGKTKNKFDMEQKISLMQLLSRLIGTHKLIVLGIYTFFLKY
LTPKQRDVTRIMSACAQACHDLVPPEVINVMVRKIADEFVSDGVANEVAAAGINTIRE
ICSRAPLAIDEILLQDLVEYKGSKAKGVNMAAKSLIALYRDVAPEMLKKKDRGKNAAM
EVQEAKKGGKDSKRPQFGADNSVQGIAGIELLAKWKKEHGEESENEDADANWEVDVDS
EEDDVDGEWVTMDSDKEYDVDMEDSDDEKDNAKGKESDSDLELSDDDDEKEVKDEQED
ADIDPEAAFREIASTRILTPADFAKLQELRNEESVAKIMGIHKQDKREELVDASTLTG
PIKYKQSREERLQKVLEGREGRDKFGSRRGKRDNMRSTTNREKERRKNFVMSIHKRSV
RGKQKMSLRDKQKVLRAHITKQKKKGY"
gene complement(944069..945860)
/gene="Brf1"
/locus_tag="P301_G13416"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 946553..947272
/gene="Cpd1"
/locus_tag="P301_G13421"
mRNA 946553..947272
/gene="Cpd1"
/locus_tag="P301_G13421"
/product="Cpd1p"
CDS 946553..947272
/gene="Cpd1"
/locus_tag="P301_G13421"
/note="Similar to YGR247W 'Cyclic nucleotide
phosphodiesterase, hydrolyzes ADP-ribose 1'', 2''-cyclic
phosphate to ADP-ribose 1''-phosphate; may have a role in
tRNA splicing; no detectable phenotype is conferred by
null mutation or by overexpression'"
/codon_start=1
/product="Cpd1p"
/protein_id="EWG91183.1"
/translation="MAIALWYCPPQGSVAYETLQMLIFSFQTLFPDSPVFEPHVTVTS
HLVCNSKDDVNKILTSCVAAIQSIRSHQTAKKGRKGQASHTVAAPLVSFNGCSVGKQY
FKKIVLECNKNKILYGVAQVMREMYVEIDPETRSSRAATWVHEEFHPHVSLLYSDIHP
VSQASLRVVQQRIEDALDVQLVPREKRKGSGNADGSNEVQMRWDFDVSSSLSWNIPGT
FKVVNCVGPVQEWEVLGRVDV"
gene 947552..948319
/gene="Sol4"
/locus_tag="P301_G13426"
mRNA 947552..948319
/gene="Sol4"
/locus_tag="P301_G13426"
/product="Sol4p"
CDS 947552..948319
/gene="Sol4"
/locus_tag="P301_G13426"
/note="Similar to YGR248W 6-phosphogluconolactonase with
similarity to Sol3p"
/codon_start=1
/product="Sol4p"
/protein_id="EWG91184.1"
/translation="MVKLQRFSEKKSLIHEFGKFILEKQDSALTGDADAVFNIAISGG
SMNQALYESLVNDKNIFPHIKWPQWRIFFCDERLVPFEDPQSNYGQFKKTVLDPLVHQ
GNQLNLGPTVYTINESLIGGGETANRKIAEEYASLLPASFDLILLGCGEDGHTCSLFP
GVEFNYLVEEMDRKILWCNNSPKAPKDRITFTLAVVAEAKSVCFLVRGAAKKAIMHDV
LIVKNSELPSVLVNEMVGTKVTWFLDDEAGALIPENC"
gene 949642..951012
/gene="Mga1"
/locus_tag="P301_G13431"
mRNA 949642..951012
/gene="Mga1"
/locus_tag="P301_G13431"
/product="Mga1p"
CDS 949642..951012
/gene="Mga1"
/locus_tag="P301_G13431"
/note="Similar to YGR249W 'Protein similar to heat shock
transcription factor; multicopy suppressor of pseudohyphal
growth defects of ammonium permease mutants'"
/codon_start=1
/product="Mga1p"
/protein_id="EWG91185.1"
/translation="MQPKTFVHQLHAILLEPEVNKWIYWSPTDNTIFFLKPYDPNFST
HVLKRYFKHGNVNSFVRQLHMYGFHKLSHPSPDQSSANNGNVKELVEWKFTHPSGFFF
KKANAGILNKIQRKSTGVGKDGKRKNILSPISVSYVDASRLNVLSQQSGPVSAREPSN
MFMGSPVHYSTSQSPPHISIPQEQQSSGPYLISSLPPQQPTVNMMRRQSISARMMNSY
DYPNQFSTQDSIVQPQQPQQVLSPQALSGPPMKKSGTLSSTDDLKTTSLPIVNYPMPY
HPGAFAQQQQQQQQPLPTVPPYSSYSTPFPSMMNSLSNSASNSPALGVCNNNVTLPKK
SNISERQALDNHIQTLKNSLSTITDLIEKHINSASQDENKTLTNDAMNKDLRTSLSLL
QNSKEEIIQLESKWMSMQSVKTTALPLQETTNTSSTLTSLTSSIIPKSIPIITKGEVA
TKPASY"
assembly_gap 952551..952606
/estimated_length=56
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(952760..955105)
/locus_tag="P301_G13441"
mRNA complement(952760..955105)
/locus_tag="P301_G13441"
/product="hypothetical protein"
CDS complement(952760..955105)
/locus_tag="P301_G13441"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91186.1"
/translation="MNIAEEPSDEVISSGPEDTDICSQQTSASAEAGDQSIKIERKTS
TGLQLEQLANTNLLTIRIKWQLQEEEDDHCNSRITDQIMDTIQHYKGISVNNSDTETY
EFLPDTRRLQVLEQNKDIYLYEHGSQEYEKSYKDNEEEDDWRYDTVLQAQFKYPKSLE
NACTDISELLKSEPIGQHIDKWSIGVNKHALTYPGNIFVGGIAKSLSIGELSFLFSKY
GPILSMKLIYDKTKGEPNGYGFISYPLGSQASLCIKELNGRTVNGSTLFINYHVERKE
RERIHWDHVKENNNDDNFRCLFIGNLPYHNPEKVETLITPKEVIEVIKKELSKKFPDF
DIISYYFPKRSNTRSSSSVSFNEEGSVESNKSSNNTNGNAQDEDILKGYGFIKLINHE
QALAAIETFNGFMWHGNRLVVNKAVQHKVYNNHNSHDRHPSISNHNDMEVLEFANNPM
YDYNNYTYDRYYFNNNKNGNSNDTSNVRYFDSVRSTPVAEKMDLFYPQRESFSEGRGQ
RVPRFMGNKFDMYQYPSTSYSLPIPMRNQQESNLYVKHIPLSWTDEDLYDFYKSFGEI
ISVKVITVGGSKNKYRQQSNDSSSDNDLPVGSSRGYGFVSFESPLDAAKAILNTDGYQ
VSKDQVLSVSFAQKRGNLSSSDDDDQSQTDNSSKFQNFQPHNDYHKAYPTKYNKKFIN
ALMTQNQSQQQVSRENYFIPLQYPNTNTKPVNSYNLISANQNNANWMMPMFPSFGFIP
QVPPVPYIIPPQNPAANHIPIMANGSNEEEEFSSGDYSMDY"
gene 957221..957805
/locus_tag="P301_G13451"
mRNA 957221..957805
/locus_tag="P301_G13451"
/product="hypothetical protein"
CDS 957221..957805
/locus_tag="P301_G13451"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91187.1"
/translation="MSRAKELQEKLNLQAKLQSTFSNNTAAVLDWLKESDETGISNDT
ERNKQLKDHKELEDGKKAFFKLPVLQIGSGLHFRTQDDASAKEDIHTIGEFIEGDKKV
SSLAKKKKRSDPGLQRNNMYRITKDDTKAMIALKRKMRKGEKEGLRKKQEHSKSSVSN
SYSASDEEDEDVGTMPQKSTKKNLVYFLIKKEGT"
gene 958449..959768
/gene="Gcn5"
/locus_tag="P301_G13456"
mRNA 958449..959768
/gene="Gcn5"
/locus_tag="P301_G13456"
/product="Gcn5p"
CDS 958449..959768
/gene="Gcn5"
/locus_tag="P301_G13456"
/note="Similar to YGR252W 'Histone acetyltransferase,
acetylates N-terminal lysines on histones H2B and H3;
catalytic subunit of the ADA and SAGA histone
acetyltransferase complexes; founding member of the
Gcn5p-related N-acetyltransferase superfamily'"
/codon_start=1
/product="Gcn5p"
/protein_id="EWG91188.1"
/translation="MVTKHQIEEDHLDGATTDPEVKRVKLENNVEEIQPEQAETNKQE
GTDKENKGKFEKETERIGGSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEF
RVVNNDNTKENMMVLTGLKNIFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGG
ITYRPFDKREFAEIVFCAISSTEQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAI
GYFKKQGFTKEITLDKSIWMGYIKDYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRR
KIRTISKSHIVRPGLEQFKDLNNIKPIDPMTIPGLKEAGWTPEMDALAQRPKRGPHDA
AIQNILTELQNHAAAWPFLQPVNKEEVPDYYDFIKEPMDLSTMEIKLESNKYQKMEDF
IYDARLVFNNCRMYNGENTSYYKYANRLEKFFNNKVKEIPEYSHLID"
gene complement(959938..960720)
/gene="Pup2"
/locus_tag="P301_G13461"
mRNA complement(959938..960720)
/gene="Pup2"
/locus_tag="P301_G13461"
/product="Pup2p"
CDS complement(959938..960720)
/gene="Pup2"
/locus_tag="P301_G13461"
/note="Similar to YGR253C 'Alpha 5 subunit of the 20S
proteasome involved in ubiquitin-dependent catabolism;
human homolog is subunit zeta'"
/codon_start=1
/product="Pup2p"
/protein_id="EWG91189.1"
/translation="MFLTRSEYDRGVSTFSPEGRLFQVEYSLEAIKLGSTAIGIATKE
GVVLGVEKRATSPLLESDSIEKIVEIDRHIGCAMSGLTADARSMIEHARTAAVTHNLY
YDEDINVESLTQSVCDLALRFGEGASGEERLMSRPFGVALLIAGHDADDGYQLFHAEP
SGTFYRYNAKAIGSGSEGAQAELLNEWHSSLTLKEAELLVLKILKQVMEEKLDENNAQ
LSCITKQDGFKIYDNEKTAELIKELKEKEAAESPEEADVEMS"
assembly_gap 961121..961479
/estimated_length=359
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 962521..963834
/gene="Eno1"
/locus_tag="P301_G13471"
mRNA 962521..963834
/gene="Eno1"
/locus_tag="P301_G13471"
/product="Eno1p"
CDS 962521..963834
/gene="Eno1"
/locus_tag="P301_G13471"
/note="Similar to YGR254W 'Enolase I, a phosphopyruvate
hydratase that catalyzes the conversion of
2-phosphoglycerate to phosphoenolpyruvate during
glycolysis and the reverse reaction during
gluconeogenesis; expression is repressed in response to
glucose'"
/codon_start=1
/product="Eno1p"
/protein_id="EWG91190.1"
/translation="MAVSKVYARSVYDSRGNPTVEVELTTEKGVFRSIVPSGASTGVH
EALEMRDGDKSKWMGKGVLHAVKNVNDVIAPAFVKANIDVKDQKAVDDFLISLDGTAN
KSKLGANAILGVSLAASRAAAAEKNVPLYKHLADLSKSKTSPYVLPVPFLNVLNGGSH
AGGALALQEFMIAPTGAKTFAEALRIGSEVYHNLKSLTKKRYGASAGNVGDEGGVAPN
IQTAEEALDLIVDAIKAAGHDGKIKIGLDCASSEFFKDGKYDLDFKNPNSDKSKWLTG
PQLADLYHSLMKRYPIVSIEDPFAEDDWEAWSHFFKTAGIQIVADDLTVTNPKRIATA
IEKKAADALLLKVNQIGTLSESIKAAQDSFAAGWGVMVSHRSGETEDTFIADLVVGLR
TGQIKTGAPARSERLAKLNQLLRIEEELGDNAVFAGENFHHGDKL"
gene complement(964117..965556)
/gene="Coq6"
/locus_tag="P301_G13476"
mRNA complement(964117..965556)
/gene="Coq6"
/locus_tag="P301_G13476"
/product="Coq6p"
CDS complement(964117..965556)
/gene="Coq6"
/locus_tag="P301_G13476"
/note="Similar to YGR255C 'Putative flavin-dependent
monooxygenase; involved in ubiquinone (Coenzyme Q)
biosynthesis; localizes to the matrix face of the
mitochondrial inner membrane in a large complex with other
ubiquinone biosynthetic enzymes; human COX6 can rescue a
yeast cox6 mutant and is implicated in steroid-resistant
nephrotic syndrome (SRNS)'"
/codon_start=1
/product="Coq6p"
/protein_id="EWG91191.1"
/translation="MFFSKVMLTRRILVRGLATAKSSAPKLTDVLIVGGGPAGLTLAA
SIKNSPQLKDLKTTLVDMVDLKDKLSDFYNSPPDYFTNRIVSVTPRSIHFLENNAGAT
LMHDRIQSYDGLYVTDGCSKATLDLARDSMLCMIEIINIQASLYNRISQYDSKKDSID
IIDNTKVVNIKHSDPNDPLSWPLVTLSNGEVYKTRLLVGADGFNSPTRRFSQIPSRGW
MYNAYGVVASMKLEYPPFKLRGWQRFLPTGPIAHLPMPENNATLVWSSSERLSRLLLS
LPPESFTALINAAFVLEDADMNYYYRTLEDGSMDTDKLIEDIKFRTEEIYATLKDESD
IDEIYPPRVVSIIDKTRARFPLKLTHADRYCTDRVALVGDAAHTTHPLAGQGLNMGQT
DVHGLVYALEKAMERGLDIGSSLSLEPFWAERYPSNNVLLGMADKLFKLYHTNFPPVV
ALRTFGLNLTNKIGPVKNMIIDTLGGNEK"
gene 965810..965881
/locus_tag="P301_G13481"
tRNA 965810..965881
/locus_tag="P301_G13481"
/product="tRNA-Thr"
/note="TT(UGU)G2"
gene 966220..967698
/gene="Gnd2"
/locus_tag="P301_G13486"
mRNA 966220..967698
/gene="Gnd2"
/locus_tag="P301_G13486"
/product="Gnd2p"
CDS 966220..967698
/gene="Gnd2"
/locus_tag="P301_G13486"
/note="Similar to YGR256W '6-phosphogluconate
dehydrogenase (decarboxylating), catalyzes an NADPH
regenerating reaction in the pentose phosphate pathway;
required for growth on D-glucono-delta-lactone'"
/codon_start=1
/product="Gnd2p"
/protein_id="EWG91192.1"
/translation="MSKAVGDLGLVGLAVMGQNLILNAADHGFTVVAYNRTQSKVDRF
LANEAKGKSIIGATSIEDLVAKLKKPRKIMLLIKAGAPVDTLIKELVPHLDKGDIIID
GGNSHFPDTNRRYEELTKQGILFVGSGVSGGEDGARFGPSLMPGGSAEAWPHIKNIFQ
SIAAKSNGEPCCEWVGPAGSGHYVKMVHNGIEYGDMQLICEAYDIMKRIGRFTDKEIS
EVFDKWNTGVLDSFLIEITRDILKFDDVDGKPLVEKIMDTAGQKGTGKWTAINALDLG
MPVTLIGEAVFARCLSAIKDERKRASKLLAAPTVPKDAIHDREQFVYDLEQALYASKI
ISYAQGFMLIREAARSYGWKLNNPAIALMWRGGCIIRSVFLAEITKAYRDDPDLENLL
FNEFFASAVTKAQSGWRRTIALAATYGIPTPAFSTALAFYDGYRSERLPANLLQAQRD
YFGAHTFRILPECASAHLPVDKDIHINWTRHGGNISSSTYQA"
gene complement(967801..968901)
/gene="Mtm1"
/locus_tag="P301_G13491"
mRNA complement(967801..968901)
/gene="Mtm1"
/locus_tag="P301_G13491"
/product="Mtm1p"
CDS complement(967801..968901)
/gene="Mtm1"
/locus_tag="P301_G13491"
/note="Similar to YGR257C Mitochondrial protein of the
mitochondrial carrier family, involved in activating
mitochondrial Sod2p probably by facilitating insertion of
an essential manganese cofactor"
/codon_start=1
/product="Mtm1p"
/protein_id="EWG91193.1"
/translation="MSDRNTSNSLTLKERMLSAGAGSVLTSLILTPMDVVRIRLQQQQ
MIPDCSCDGAAEVPNAISSGSKMKTFTNVGGQNLNNAKIFWESACFQELHCKNSSLKF
NGTLEAFTKIASVEGITSLWRGISLTLLMAIPANMVYFSGYEYIRDVSPIASTYPTLN
PLFCGAIARVFAATSIAPLELVKTKLQSIPRSSKSTKTWMMVKDLLNETRQEMKMVGP
SRALFKGLEITLWRDVPFSAIYWSSYELCKERLWLDSTRFASKDANWVHFINSFASGC
ISGMIAAICTHPFDVGKTRWQISMMNNSDPKGGNRSRNMFKFLETIWRTEGLAALYTG
LAARVIKIAPSCAIMISSYEISKKVFGNKLHQ"
gene complement(969267..972362)
/gene="Rad2"
/locus_tag="P301_G13496"
mRNA complement(969267..972362)
/gene="Rad2"
/locus_tag="P301_G13496"
/product="Rad2p"
CDS complement(969267..972362)
/gene="Rad2"
/locus_tag="P301_G13496"
/note="Similar to YGR258C 'Single-stranded DNA
endonuclease, cleaves single-stranded DNA during
nucleotide excision repair to excise damaged DNA; subunit
of Nucleotide Excision Repair Factor 3 (NEF3); homolog of
human XPG protein'"
/codon_start=1
/product="Rad2p"
/protein_id="EWG91194.1"
/translation="MGVHSFWDIAGPTARPVRLESLEDKRMAVDASIWIYQFLKAVRD
QEGNAVKNSHITGFFRRICKLLYFGIRPVFVFDGGVPVLKRETIRQRKERRQGKRESA
KSTARKLLALQLQNGSNDNVKNSTPSSGSSVQIFKPQDEWDLPDIPGFKYDKEDARVN
SNKTFEKLMNSINGDGLEDIDLDTINPASAEFEELPKATQYLILSSLRLKSRLRMGYS
KEQLETIFPNSMDFSRFQIDMVKRRNFFTQKLINTTGFQDGGASKLNEEVINRISGQK
SKEYKLTKTNNGWILGLGANDGSDAQKAIVIDDKDAGALVKQLDSNAEDGDVLRWDDL
EDNSLKIVRHESSNATTAPQKRSNRSEDEGCDSDECEWEEVELKPKNVKFVEDFSLKA
ARLPYMGQSLNNAGSKSFLDKRHDQASPTKTIPTMRISRISVEDDDEDYLKQIEEIEM
MEAVQLSKMEKKPEADDKSKIAKPVTSKGTEARPPIVQYGLLGAQPDSKQPHHVTNLN
SKSESVIKRTSKTVLSEFRPPSQQEDKGAILTEGEQNLNFISHKIPQFDFNNKNSLLF
QKNTESNVSQEATKEKSPIPEMPSWFSSTASQQLYNPYNTTNFVEDKNVRNEQESGAE
TTNKGSSYELLTGLNATEILERESEKESSNDENKDDDLEVLSEELFEDVPTKSQISKE
AEDSDSRKVESINKEHRKPLIFDYDFSEDEEDNIVENMIKEQEEFDTFKNTTLSTSAE
RNVAENAFVEDELFEQQMKDKRDSDEVTMDMIKEVQELLSRFGIPYITAPMEAEAQCA
ELLQLNLVDGIITDDSDVFLFGGTKIYKNMFHEKNYVEFYDAESILKLLGLDRKNMIE
LAQLLGSDYTNGLKGMGPVSSIEVIAEFGNLKNFKDWYNNGQFDKRKQETENKFEKDL
RKKLVNNEIILDDDFPSVMVYDAYMRPEVDHDTTPFVWGVPDLDMLRSFMKTQLGWPH
EKSDEILIPLIRDVNKRKKKGKQKRINEFFPREYISGDKKLNTSKRISTATGKLKKRK
M"
gene 974072..975676
/gene="Tna1"
/locus_tag="P301_G13506"
mRNA 974072..975676
/gene="Tna1"
/locus_tag="P301_G13506"
/product="Tna1p"
CDS 974072..975676
/gene="Tna1"
/locus_tag="P301_G13506"
/note="Similar to YGR260W 'High affinity nicotinic acid
plasma membrane permease, responsible for uptake of low
levels of nicotinic acid; expression of the gene increases
in the absence of extracellular nicotinic acid or
para-aminobenzoate (PABA)'"
/codon_start=1
/product="Tna1p"
/protein_id="EWG91195.1"
/translation="MSNKFTMESPKHLVDDVLFISPTNDGSEEKPTEVTFQEDEGHDA
SLHNRSHDKKSELATEREIMATTTDDDGIPSPSHPMEKRVLRKMDIYLIPLMGMLYFL
SNLDKSNIGNAEVAGLSKDIHLVGTQYNTCVTVFFATYVLFDPIGTNLLKIMGPPLMM
SICLTCFGAISLGTAWVKNYAQLIVVRLLLGAFEGMIYPAINMYLSVCYRREQYALRF
AFVFSAACLSSSFGGLIAYGCSKISGSLKDWQYIYIVEGCISLGFVPFYAFGLSKNLE
DSWFFNKEEKEYISERYKTMNTFDPDEKFEWFQVWQAVKDVKTWASAVALFGIDLTTF
GLTVFLPIIITSMGFTNVRAQLMTVPIYFLTAIVFFICAVWSDRIKLRSPFILGACLT
TSIGIAIVLGSQVHGVRYFGVYILCMGIYVNAACNCLWLSGNTGNYFKRATALGINLF
FGSGSGLVSGQIFVAKDKPRYIKGLSISLAFQVFSIFMTVVQIFLYKRENDKKKAIID
RCNELGEPIPYDERLSDKNPEFKYMY"
gene complement(975908..978337)
/gene="Apl6"
/locus_tag="P301_G13511"
mRNA complement(975908..978337)
/gene="Apl6"
/locus_tag="P301_G13511"
/product="Apl6p"
CDS complement(975908..978337)
/gene="Apl6"
/locus_tag="P301_G13511"
/note="Similar to YGR261C 'Beta3-like subunit of the yeast
AP-3 complex; functions in transport of alkaline
phosphatase to the vacuole via the alternate pathway;
exists in both cytosolic and peripherally associated
membrane-bound pools'"
/codon_start=1
/product="Apl6p"
/protein_id="EWG91196.1"
/translation="MVDSIHRIASALDTAKVITREAAAVATSKLGESSYTYYSQNINP
QQLVTLLNSRNSREVRDAMKRIISIMASDDDSIDVQLYFADVVKNITTNDTKVKRLIH
LYLLRFAENDPNLTLLSINSLQKSLSDSNSELRCFALSALSDMKMSSLAPIILHTVKK
LVTDPSAMVRGEVALAIIKLYRAGKNDYHEELLDILKELMADTDPKVISCAVLAYKEC
YADHLELLHGHFRRYCRIIKQLDSWSQSYLIELLIKYCKQYLPKPTVVDKSSEGSPRS
CPLPDKYNEIEYPSYEVVNDPDLDLFLQSLNCLIYSSNPTVILSCCNALYQLASPLQM
KNTKFIEALVRTVTMTENQGNKEMLLQAIHFLSILDQTLFLPYTKKFYVFPKDPIVAS
IWKIQILSTLINESNVKEIFKELKYYVASAHFPENVVIMAVKSLSRCGQLSTSWESHV
MKWLIDHMESHNLSASVLDAYVNVIRMLVQKNPTKHLRIIFKLADLLTVQTSLADNAR
AGIVWLFGEIASIEFKICPDVLRRLIQNFSNEGPETRCQILVLSAKLLSYDIDNFKQA
QVTGSEENNQNPPYYDFSGSRISQMYNAVLYLAKYDDEFDIRDRARMISSLFDSGKYE
IVSLLLQAPKPTARSDDFIVSARLETHTPEIKEFFRMLPWNTEITEVGETGNDIREGA
ELKDYNKYKKSFSSQSFITNNSARSFTSSSNAKLTGINDGDSNSISGKGNVNTFTSQN
GKKYRLQSLDEFFSDIPERKSKPRKIIKVVEESSDEDEDESEESSDDDEYSDSSLGTS
SSGTSSSHLEL"
gene complement(978561..979346)
/gene="Bud32"
/locus_tag="P301_G13516"
mRNA complement(978561..979346)
/gene="Bud32"
/locus_tag="P301_G13516"
/product="Bud32p"
CDS complement(978561..979346)
/gene="Bud32"
/locus_tag="P301_G13516"
/note="Similar to YGR262C 'Protein kinase, component of
the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and
Gon7p; EKC/KEOPS complex is required for t6A tRNA
modification and may have roles in telomere maintenance
and transcription'"
/codon_start=1
/product="Bud32p"
/protein_id="EWG91197.1"
/translation="MTQEFIDKVSSYLTPDVDIAPISQGAEAIVFTTTTHPYLPRAKD
SHQKYIIKYRPPKRYRHPQIDQALTKHRTLNESRLLAKLYLIPGLCVPQLIACDPYNG
FIWLEFLGEDLPGGHGFSNLKNFLWMHDQDPYSDLVATTLRKVGRQIGLLHWNDYCHG
DLTSSNIVLVRDGARWTPHLIDFGLGSVSNLVEDKGVDLYVLERAILSTHSKHAEKYN
AWIMEGFEEVYREQGAKGAKKLKEVTKRFEEVRLRGRKRSMLG"
gene complement(979551..980825)
/gene="Say1"
/locus_tag="P301_G13521"
mRNA complement(979551..980825)
/gene="Say1"
/locus_tag="P301_G13521"
/product="Say1p"
CDS complement(979551..980825)
/gene="Say1"
/locus_tag="P301_G13521"
/note="Similar to YGR263C 'Sterol deacetylase; component
of the sterol acetylation/deacetylation cycle along with
Atf2p; integral membrane protein with active site in the
ER lumen; green fluorescent protein (GFP)-fusion protein
localizes to the endoplasmic reticulum'"
/codon_start=1
/product="Say1p"
/protein_id="EWG91198.1"
/translation="MAANSGLDSKVEYYRLQENEIISAVSSEDADQNDAGFRLSTIHL
HLFHGLKFAALLFTVVPVFIILDSMKIIFQRKRRFCLDHVNRSFLRQSSWILDERICQ
YVLNPLFVCLYPSTFSSPTYVKCNIPIEDQKSPENNIFQDHQLNAPKIVSTKFYQYVM
PEGFDPTTDPVLVFYHGGGYALKLTPTSFSFLNNMRNAFPKMAILVPDYTVTATDDQS
KKYPLQILQNVAIFDYVVKTMGCKNVVIMGDSAGGNAVLNIVLYLRKCHREIYPKKVI
AISPWANATFFHEGEKEYMQGTQEWDGLCLKSHSMFGRMFVGNNPNVDFTSDPFVNIE
KNFETKMWQDILKKCSVMITYGSDELLSFQNKILAKKMSDASEGCNHFTAKNVLVEHQ
GYHTGPILNYSRNMDRWTNIPSIARILEFMQS"
gene complement(981182..983438)
/gene="Mes1"
/locus_tag="P301_G13531"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 984239..986344
/locus_tag="P301_G13541"
mRNA 984239..986344
/locus_tag="P301_G13541"
/product="hypothetical protein"
CDS 984239..986344
/locus_tag="P301_G13541"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91199.1"
/translation="MHATNWFDDWNPEALYRDDVTGCDDCSETSPIPKSGIICGPILR
LINMDFKEKTYEGSIMVVVRGEENFPKITYQLGPSLPSEDEDIEVNEAFFEGKLFHKD
ILKDDNIWFYRYEIKLPMSNYEQMVKYAVDGTMEPHYRFFVPSFTQNSNVISYSCNGF
SLSVDTSKFKGSLWYDVLKKHRYVHYHAILGGGDQIYSDNIKLHAPNLKAWLETKDPI
KKYNTQTTEETKEQIRQFYLEHYLNWYGYGHWYGSTPKSKTTQKCFVKSLACIPAINV
WDDHDIIDGYGSYNDSFMKTENFLTVGRMAYRYYMLFQQHVSASKQDGDEYAYLKSKQ
WILGNEKGSSYIGERSHSIFSWLGPKMAMLGLDCRTERKLHEIFSERSYSLIWERVER
EIKNLKGGHLLLMLGIPIAYPRLVWLEWLFTSKLLAPIKYLSKKGIFASGFVNEFNGD
VELLDDLNDHWCARHHKKERNYLIMKLQDIGAKYGVRITILSGDVHLASVGRFRAKIH
RHHLIMSEEKEKENTRIIEEPTKDVRLIFNIIASAIVNTPPPDAMATLLQKRCRLHHF
DLETDEDAVPIFAKEVDGVHKRKESCFMNKRNWSDIIPIENLLNNPQLSKELGVKVGD
IVIPGIITEQQKLQKLENDDQINSYPVTSGGLFTTIHVERDANQTNSQTVSYCLPIPE
LTVTCERLSHKGIKHLNIT"
gene complement(986586..987317)
/gene="Fol2"
/locus_tag="P301_G13546"
mRNA complement(986586..987317)
/gene="Fol2"
/locus_tag="P301_G13546"
/product="Fol2p"
CDS complement(986586..987317)
/gene="Fol2"
/locus_tag="P301_G13546"
/note="Similar to YGR267C GTP-cyclohydrolase I, catalyzes
the first step in the folic acid biosynthetic pathway"
/codon_start=1
/product="Fol2p"
/protein_id="EWG91200.1"
/translation="MHNIQLVQEIERHETPLNIRPTSPYTLNPPVERDGFSWPSVGTR
QRAEETEEEEKERIQRISGAIKTILTELGEDVNREGLLDTPQRYAKAMLYFTKGYQTN
IMDDVIKNAVFEEDHDEMVIVRDIEIYSLCEHHLVPFFGKVHIGYIPNKKVIGLSKLA
RLAEMYARRLQVQERLTKQIAMALSDILKPLGVAVVMEASHMCMVSRGIQKTGSSTVT
SCMLGGFRAHKTREEFLTLLGRRSI"
gene complement(987639..988235)
/gene="Hua1"
/locus_tag="P301_G13551"
mRNA complement(987639..988235)
/gene="Hua1"
/locus_tag="P301_G13551"
/product="Hua1p"
CDS complement(987639..988235)
/gene="Hua1"
/locus_tag="P301_G13551"
/note="Similar to YGR268C 'Cytoplasmic protein containing
a zinc finger domain with sequence similarity to that of
Type I J-proteins; computational analysis of large-scale
protein-protein interaction data suggests a possible role
in actin patch assembly'"
/codon_start=1
/product="Hua1p"
/protein_id="EWG91201.1"
/translation="MSKDTHDDELPSYEDVIKEEERLQSQPPRPPRPAANLAQGHQSR
PHQRPSTMPATSSSQTYAHSHSYTPTSSQPRPPPRPQQNPSLPWTYPPRFYCSKCGNT
GYKLKNGRSCKSCWRRFAPQNNVVSAPTYYTNYTMPVYTNAWQGNRPLYVQPGDPRLG
GVLCGECRGSGRTRFLLDEDICPLCHGVGRIITQPQRY"
gene 988952..992952
/gene="Yta7"
/locus_tag="P301_G13566"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(988957..989175)
/locus_tag="P301_G13571"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 993371..999274
/gene="Slh1"
/locus_tag="P301_G13576"
mRNA 993371..999274
/gene="Slh1"
/locus_tag="P301_G13576"
/product="Slh1p"
CDS 993371..999274
/gene="Slh1"
/locus_tag="P301_G13576"
/note="Similar to YGR271W 'Putative RNA helicase related
to Ski2p, involved in translation inhibition of
non-poly(A) mRNAs; required for repressing propagation of
dsRNA viruses'"
/codon_start=1
/product="Slh1p"
/protein_id="EWG91202.1"
/translation="MSTEYSADSSKSFMIAMQSMIDTSQTFNLDRSKISLPDFDDELK
KVQKDEQNQRTELTVLSQDRNDWDDIFEEFKDISFAQLQSIIDSYKTKNAVAVYKKIG
KLINEAETTLSSNVLLETVLQMVYKHQKQELEKELLDFLGTGNIDLVSLLLQHRRMIV
ATPIETTILLIKNAVNSTPEFLTQQDIRNQVLKSAEDAKNRKLNPATKIIKYPHVFRK
YEAGSTTAMAFAGQKFTLPVGTTRMSYNTHEEIIIPAADQASNKNYLYTKLLKISDLD
HFCKTVFPYETLNQIQSLVYPVAYKTNENMLICAPTGAGKTDIALLTIINTIKQFSVV
NGENEIDIQYDDFKVIYVAPLKALAAEIVDKFSKKLAPFNIQVRELTGDMQLTKAEIL
ATQVIVTTPEKWDVVTRKANGDNDLVSKVKLLIIDEVHLLHEDRGSVIETLVARTLRQ
VESSQSMIRIIGLSATLPNFMDVADFLGVNRQIGMFYFDQSFRPKPLEQQLLGCRGKA
GSRQSKENIDKVAYDKLSEMIQRGYQVMVFVHSRKETVKSARNFIKLAESNHEVDLFA
PDPIEKDKYSRSLVKNRDKDMKEIFQFGFGIHHAGMARSDRNLTEKMFKDGAIKVLCC
TATLAWGVNLPADCVIIKGTQVYDSKKGGFIDLGISDVIQIFGRGGRPGFGSANGTGI
LCTSNDRLDHYVSLITQQHPIESRFGSKLVDNLNAEISLGSVTNVDEAIEWLGYTYMF
VRMRKNPFTYGIDWEEIANDPQLYERRRKMIVVAARRLHALQMIVFDEVSMHFIAKDL
GRVSSDFYLLNESVEIFNQMCDPRATEADVLSMISMSSEFDGIKFREEESKELKRLSD
ESVECQIGSQLDTPQGKANVLLQAYISQTRIFDSALSSDSNYVAQNSVRICRALFLIG
VNRRWGKFSNVMLNICKSIEKRLWAFDHPLCQFDLPENIIRRIRDTKPSMEHLLELEA
DELGELVHNKKAGSRLYKILSRFPKINIEAEIFPITTNVMRIHIALGPDFVWDSRIHG
DAQFFWVFVEESDKSQILHFEKFILNRRQLNNQHEMDFMIPLSDPLPPQVVVKVVSDT
WIGCESTHAISFQHLIRPFNETLQTKLLKLRPLPTSALQNPLIESIYPFKYFNPMQTM
TFYTLYNTNENAFVGSPTGSGKTIVAELAIWHAFKTFPGKKIVYIAPMKALVRERVDD
WRKKITPVTGDKVVELTGDSLPDPKDVHDATIVITTPEKFDGISRNWQTRKFVQDVSL
IIMDEIHLLASDRGPILEMIVSRMNYISSQTKQPVRLLGMSTAVSNAYDMAGWLGVKD
HGLYNFPSSVRPVPLKMYIDGFPDNLAFCPLMKTMNKPVFMAIKQHSPDKPALIFVAS
RRQTRLTALDLIHLCGMEDNPRRFLNIDDEEELQYYLSQVTDDTLKLSLQFGIGLHHA
GLVQKDRSISHQLFQKNKIQILIATSTLAWGVNLPAHLVIIKGTQFFDAKIEGYRDMD
LTDILQMMGRAGRPAYDTTGTAIVYTKESKKMFYKHFLNVGFPVESSLHKVLDDHLGA
EITSGSITNKQEALDFLSWTFLFRRAHHNPTYYGIEDDTSTAGVSEHLSSLIDSTLEN
LRESQCVLLHGDDIVATPFLSISSYYYISHLTIRQLLKQIHDHATFQEVLRWLSLAVE
YNELPVRGGEIIMNEEMSQQSRYSVESTFTDEFELPMWDPHVKTFLLLQAHLSRVDLP
IADYIQDTVSVLDQSLRILQAYIDVASELGYFHTVLTMIKMMQCIKQGYWYEDDPVSV
LPGLQLRRIKDYTFSEQGFIEMTPQQKKKKLLTLEEIGRFGYKKLLNVFDQLTFGMTE
SEDTKKRFVSVCQRLPVLEGMKFEEQENNEVLTFYSKHLSSKHNNGFEVYCDKFPKIQ
KELWFLIGHKGDELLMIKRCQPKQMNKEVIIHCDLFIPEEIRGEELQFSLINDALGLR
YDMVHKLIS"
gene complement(999380..1000081)
/gene="Efg1"
/locus_tag="P301_G13581"
mRNA complement(999380..1000081)
/gene="Efg1"
/locus_tag="P301_G13581"
/product="Efg1p"
CDS complement(999380..1000081)
/gene="Efg1"
/locus_tag="P301_G13581"
/note="Similar to YGR271C-A 'Essential protein required
for maturation of 18S rRNA; null mutant is sensitive to
hydroxyurea and is delayed in recovering from alpha-factor
arrest; green fluorescent protein (GFP)-fusion protein
localizes to the nucleolus'"
/codon_start=1
/product="Efg1p"
/protein_id="EWG91203.1"
/translation="MAKLQRKRSKALGSSLEMSQIMDAGTNKIKRRIRDLERLLKKKK
DILPSTVIIEKERNLQALRLELQNNELKNKIKXXXXXXXXXXXXXXXXXXXXXXXXXX
KIKESGADDKDLQQKLRATKIELCYVINFPKTEKYIALYPNDTPSTDPKGVELTNLRR
EQFLKLVAERMDANTLNVSFEEILKGKKLDEDSIGLTLSPDKDHEDGSQVSPTQDRKE
LDQVVGEDEKDDFFE"
assembly_gap 999776..999853
/estimated_length=78
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(1000255..1000818)
/locus_tag="P301_G13586"
mRNA complement(1000255..1000818)
/locus_tag="P301_G13586"
/product="hypothetical protein"
CDS complement(1000255..1000818)
/locus_tag="P301_G13586"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91204.1"
/translation="MQHFESSDKIEKDDDTSRIKLRSSSLAAPILEAVQEAQPFEEAT
FSNLQKIHPLTENSTCNGYVIYDKDGNLKSMKDTFGRNIKTPDISNPTRARNERPLDT
IRGFEYSITKDPRWLQELETSKLGFKPRPGFAVINQDSQASINLSQLEEKVMESQKKR
RKDTYHGFLGYSADEYKDKYAKIIKSA"
gene complement(1001474..1004674)
/gene="Taf1"
/locus_tag="P301_G13591"
mRNA complement(1001474..1004674)
/gene="Taf1"
/locus_tag="P301_G13591"
/product="Taf1p"
CDS complement(1001474..1004674)
/gene="Taf1"
/locus_tag="P301_G13591"
/note="Similar to YGR274C 'TFIID subunit (145 kDa),
involved in RNA polymerase II transcription initiation;
possesses in vitro histone acetyltransferase activity but
its role in vivo appears to be minor; involved in promoter
binding and G1/S progression'"
/codon_start=1
/product="Taf1p"
/protein_id="EWG91205.1"
/translation="MVKQQGSGKTNLANEDEAYEAIFGGEFGSLEIGSYIGGDEGANS
KDYTEHLPDAVDFEDEDELADDDDDLPEESDANLHPAMMTMGAYDDVNENGAVLGIDS
NSLNMQLPEINGDLSQQFILEDDGGTPATSNALFMGMDANEIHLATETGVLDGSGANE
IGHSQLSIGGVNGNDMSINGGFIMEPDMSDGKHKKATKLDLINHEKYLLKKYFPDFEK
GKILKWNKLIYRRSVPYHWHSEISRVKKPFMPLNLKFKVQQDDKRLFNSRTISYVAPI
YQGKNNLLQSNSSASRRGLIHVSIDELFPIKEQQKKRKIIHDEKTISEDLLIATDDWD
QEKIINQGTSSTVTLADSSMTPNLKFSGGYKLKSLIEDVAEDWQWDEDMIIDAKLKES
KHAELNMNDEKLLLMIEKTNNLAQQKQQLDSSNLILPLNETILQQKFNLSNDDKYQIL
KKTHQTKVRSTISNLNIQHSQPAINLQSPFYKVAVPRYQLRHFHRENFGSHIRPGTKI
VFSKLKARKRKRDKGKDVKESFSTSQDLTIGDTAPVYLMEYSEQTPVALSKFGMANKL
INYYRKANEQDTLRPKLPVGETHVLGVQDKSPFWNFGFVEPGHIVPTLYNNMIRAPVF
KHDISGTDFLLTKSSGFGISNRFYLRNINHLFTVGQTFPVEEIPGPNSRKVTSMKATR
LKMIIYRILNHNHSKAISIDPIAKHFPDQDYGQNRQKVKEFMKYQRDGPEKGLWRLKD
DEKLLDNEAVKSLITPEQISQVESMSQGLQFQEDNEAYNFDSKLKSLEENLLPWNITK
NFINSTQMRAMIQIHGVGDPTGCGEGFSFLKTSMKGGFVKSGSPSSNNNSSNKKGTNT
HSYNVAQQQKAYDEEIAKTWYTHTKSLSISNPFEEMTNPDEINQTNKHVKTDRDDKKI
LKIVRKKRDENGIIQRQTIFIRDPRVIQGYIKIKEQDKEDVNKLLEEDTSKINNLEEL
EKQKKLLQLELANLEKSQQRRAARQNSKRNGGATRTENSVDNGSDLAGVTDGKAARNK
GKNTTRRCATCGQIGHIRTNKSCPMYSSKDNPASPK"
gene 1004855..1005328
/gene="Rtt102"
/locus_tag="P301_G13596"
mRNA 1004855..1005328
/gene="Rtt102"
/locus_tag="P301_G13596"
/product="Rtt102p"
CDS 1004855..1005328
/gene="Rtt102"
/locus_tag="P301_G13596"
/note="Similar to YGR275W 'Component of both the SWI/SNF
and RSC chromatin remodeling complexes, suggested role in
chromosome maintenance; possible weak regulator of Ty1
transposition'"
/codon_start=1
/product="Rtt102p"
/protein_id="EWG91206.1"
/translation="MDPQTLITKANKVSYYGNPTSKESWRYDWYQPSKVSSNVQQPQQ
QLGDMENNLEKYPFRYKTWLRNQEDEKNLQRESCEDILDLKEFDRRILKKSLMTSHTK
GDTSKATGAPSANQGDEALSVDDIRGAVGNSEAIPGLSAGVNNDNTKESKDVKMN"
gene complement(1005396..1007056)
/gene="Rnh70"
/locus_tag="P301_G13601"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(1007220..1008137)
/locus_tag="P301_G13606"
mRNA complement(1007220..1008137)
/locus_tag="P301_G13606"
/product="hypothetical protein"
CDS complement(1007220..1008137)
/locus_tag="P301_G13606"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91207.1"
/translation="MVEENSRVLIVLPYTPPSATLQRIIGQTIPFLRECQSQLDIVIV
PEFKTSFQLDSALGKMYSITRDVLLGYGMINSGINIIFNNTHFVESNLQWKVVLLPQE
STFETWKLELGQGQYHSIEHYALHDNIMEEIEGPKDANKFHVTALGGTFDHIHDGHKI
LLSVSTFITSQRLICGITCDELLQNKKYKELIEPYDTRCRHVHQFIKLLKPDLSVELV
PLRDVCGPTGKVPEIECLVVSRETVSGAETVNKTRIEKGMSPLAVHVVNVLGGREEDG
WSEKLSSTEIRRLLKSSASPTCTPQNPCV"
gene 1008307..1010082
/gene="Cwc22"
/locus_tag="P301_G13616"
mRNA 1008307..1010082
/gene="Cwc22"
/locus_tag="P301_G13616"
/product="Cwc22p"
CDS 1008307..1010082
/gene="Cwc22"
/locus_tag="P301_G13616"
/note="Similar to YGR278W 'Spliceosome-associated protein
that is required for pre-mRNA splicing; necessary for
Prp2p function at the first catalytic step of splicing;
has similarity to S. pombe Cwf22p; CWC22 is an essential
protein'"
/codon_start=1
/product="Cwc22p"
/protein_id="EWG91208.1"
/translation="MSTATIQDEDIKFQRENWEMIRSHVSPIISNLTMDNLQESHRDL
FQVNILIGRNIICKNVVDFTLNKQNGRLIPALSALIALLNSDIPDIGETLAKELMLMF
VQQFNRKDYVSCGNILQCLSILFLYDVIHEIVILQILLLLLEKNSLRLVIAVMKICGW
KLALVSKKTHDMIWEKLRYILQTQELSSTLRESLETLFEIRQKDYKSGSQGLFILDPT
SYTVHTHSYIVSDEDEANKELGNFEKCENFNELTMAFDTLRQKLLINNTSNTNEGSNS
QLQIYDMTSTNDVEFKKKIYLVLKSSLSGDEAAHKLLKLKIANNLKKSVVDIIIKSSL
QESTFSKFYSILSERMITFHRSWQTAYNETFEQNYTQDIEDYETDQLRILGKFWGHLI
SYEFLPMDCLKIIKLTEEESCPQGRIFIKFLFQELVNELGLDELQLRLNSSKLDGMFP
LEGDAEHIRYSINFFTAIGLGLLTEDMRSRLTIIQEVEDAEEEEKKLREEEELEKLRK
KARESQPTQGPKIHESRLFLQKDTRENSRSRSPFTVETRKRARSRTPPRGSRNHRNRS
RTPPRRPKNHRNRSRTPPARRQRHR"
gene complement(1010410..1011570)
/gene="Scw4"
/locus_tag="P301_G13621"
mRNA complement(1010410..1011570)
/gene="Scw4"
/locus_tag="P301_G13621"
/product="Scw4p"
CDS complement(1010410..1011570)
/gene="Scw4"
/locus_tag="P301_G13621"
/note="Similar to YGR279C 'Cell wall protein with
similarity to glucanases; scw4 scw10 double mutants
exhibit defects in mating'"
/codon_start=1
/product="Scw4p"
/protein_id="EWG91209.1"
/translation="MRLSNLIASASLLSAATLAAPANHEHKDKRAVVTTTVQKQTTII
VNGAASTPVAALEENAVVNSAPAAATSTTSSAASVATAAASSSENNSQVSAAASPASS
SAATSTQSSSSSQASSSSSSGEDVSSFASGVRGITYTPYESSGACKSASEVASDLAQL
TDFPVIRLYGTDCNQVENVFKAKASNQKVFLGIYYVDQIQDGVDTIKSAVESYGSWDD
VTTVSIGNELVNGNQATPSQVGQYIDSGRSALKAAGYTGPVVSVDTFIAVINNPELCD
YSDYMAVNAHAYFDKNTVAQDSGKWLLEQIQRVWTACDGKKNVVITESGWPSKGETYG
VAVPSKENQKDAVSAITSSCGADTFLFTAFNDYWKADGAYGVEKYWGVLSNE"
gene complement(1012518..1013333)
/gene="Pxr1"
/locus_tag="P301_G13631"
mRNA complement(1012518..1013333)
/gene="Pxr1"
/locus_tag="P301_G13631"
/product="Pxr1p"
CDS complement(1012518..1013333)
/gene="Pxr1"
/locus_tag="P301_G13631"
/note="Similar to YGR280C 'Essential protein involved in
rRNA and snoRNA maturation; competes with TLC1 RNA for
binding to Est2p, suggesting a role in negative regulation
of telomerase; human homolog inhibits telomerase; contains
a G-patch RNA interacting domain'"
/codon_start=1
/product="Pxr1p"
/protein_id="EWG91210.1"
/translation="MGLAATRTKQRFGLDPRNTAWSNDTSRFGHQFLEKFGWKPGMGL
GLSPMNSNTSHIKVSIKDDNVGLGAKLKRKDKKDEFDNGECAGLDVFQRILGRLNGKE
SKISEELDTQRKQKIIDGKWGIHFVKGEVLASTWDPKTHKLRNYSNAKKRKREGDDSE
DEDDDDKEDKESDKKKHKKHKKHKKDKKKDKKDKKEHKKHKKEEKRLKKEKRAEKTKE
TKKTSKLKSSESASNIPDAVNTRLSVRSKWIKQKRAALMDSKALNEIFMITND"
gene 1014423..1018856
/gene="Yor1"
/locus_tag="P301_G13636"
mRNA 1014423..1018856
/gene="Yor1"
/locus_tag="P301_G13636"
/product="Yor1p"
CDS 1014423..1018856
/gene="Yor1"
/locus_tag="P301_G13636"
/note="Similar to YGR281W 'Plasma membrane ATP-binding
cassette (ABC) transporter, multidrug transporter mediates
export of many different organic anions including
oligomycin; similar to human cystic fibrosis transmembrane
receptor (CFTR)'"
/codon_start=1
/product="Yor1p"
/protein_id="EWG91211.1"
/translation="MTITVGDAVSETELENKSQNVVLSPKASASSDISTDVDKDTSSS
WDDKSLLPTGEYIVDRNKPQTYLNSDDIEKVTESDIFPQKRLFSFLHSKKIPEVPQTD
DERKIYPLFHTNIISNMFFWWVLPILRVGYKRTIQPNDLFKMDPRMSIETLYDDFEKN
MIYYFEKTRKKYRKRHPEATEEEVMENAKLPKHTVLRALLFTFKKQYFMSIVFAILAN
CTSGFNPMITKRLIEFVEEKAIFHSMHVNKGIGYAIGACLMMFVNGLTFNHFFHTSQL
TGVQAKSILTKAAMKKMFNASNYARHCFPNGKVTSFVTTDLARIEFALSFQPFLAGFP
AILAICIVLLIVNLGPIALVGIGIFFGGFFISLFAFKLILGFRIAANIFTDARVTMMR
EVLNNIKMIKYYTWEDAYEKNIQDIRTKEISKVRKMQLSRNFLIAMAMSLPSIASLVT
FLAMYKVNKGGRQPGNIFASLSLFQVLSLQMFFLPIAIGTGIDMIIGLGRLQSLLEAP
EDDPNQMIEMKPSPGFDPKLALKMTHCSFEWEDYELNDAIEEAKGEAKDEGKKNKKKR
KDTWGKPSASTNKAKRLDNMLKDRDGPEDLEKTSFRGFKDLNFDIKKGEFIMITGPIG
TGKSSLLNAMAGSMRKTDGKVEVNGDLLMCGYPWIQNASVRDNIIFGSPFNKEKYDEV
VRVCSLKADLDILPAGDMTEIGERGITLSGGQKARINLARSVYKKKDIYLFDDVLSAV
DSRVGKHIMDECLTGMLANKTRILATHQLSLIERASRVIVLGTDGQVDIGTVDELKAR
NQTLINLLQFSSQNSEKEDEEQEAVVAGELGQLKYESEVKELTELKKKATEMSQTANS
GKIVADGHTSSKEERAVNSISLKIYREYIKAAVGKWGFIALPLYAILVVGTTFCSLFS
SVWLSYWTENKFKNRPPSFYMGLYSFFVFAAFIFMNGQFTILCAMGIMASKWLNLRAV
KRILHTPMSYIDTTPLGRILNRFTKDTDSLDNELTESLRLMTSQFTNIVGVCVMCIVY
LPWFAIAIPFLLVIFVLIADHYQSSGREIKRLEAVQRSFVYNNLNEVLGGMDTIKAYR
SQERFLAKSDFLINKMNEAGYLVVVLQRWVGIFLDMVAIAFALIITLLCVTRAFPISA
ASVGVLLTYVLQLPGLLNTILRAMTQTENDMNSAERLVTYATELPLEASYRKPEMTPP
ESWPSMGEIIFENVDFAYRPGLPIVLKNLNLNIKSGEKIGICGRTGAGKSTIMSALYR
LNELTAGKILIDNVDISQLGLFDLRRKLAIIPQDPVLFRGTIRKNLDPFNERTDDELW
DALVRGGAIAKDDLPEVKLQKPDENGTHGRMHKFHLDQAVEEEGSNFSLGERQLLALT
RALVRQSKILILDEATSSVDYETDGKIQTRIVEEFGDCTILCIAHRLKTIVNYDRILV
LEKGEVAEFDTPWTLFSQEDSIFRSMCSRSGIVENDFENRS"
gene complement(1019381..1020322)
/gene="Bgl2"
/locus_tag="P301_G13641"
mRNA complement(1019381..1020322)
/gene="Bgl2"
/locus_tag="P301_G13641"
/product="Bgl2p"
CDS complement(1019381..1020322)
/gene="Bgl2"
/locus_tag="P301_G13641"
/note="Similar to YGR282C Endo-beta-1,3-glucanase, major
protein of the cell wall, involved in cell wall
maintenance"
/codon_start=1
/product="Bgl2p"
/protein_id="EWG91212.1"
/translation="MRFSTTLATAATALFFTASQVSAIGELAFNLGVKNNDGTCKSTS
DYETELQALKSYTSTVKVYAASDCNTLQNLGPAAEAEGFTIFVGVWPTDDSHYAAEKA
ALQTYLPKIKESTVAGFLVGSEALYRNDLTASQLSDKINDVRSVVADISDSDGKSYSG
KQVGTVDSWNVLVAGYNSAVIEASDFVMANAFSYWQGQTMQNASYSFFDDIMQALQVI
QSTKGSTDITFWVGETGWPTDGTNFESSYPSVDNAKQFWKEGICSMRAWGVNVIVFEA
FDEDWKPNTSGTSDVEKHWGVFTSSDNLKYSLDCDFS"
gene complement(1020613..1021637)
/locus_tag="P301_G13646"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(1022255..1023187)
/gene="Erv29"
/locus_tag="P301_G13661"
mRNA complement(1022255..1023187)
/gene="Erv29"
/locus_tag="P301_G13661"
/product="Erv29p"
CDS complement(1022255..1023187)
/gene="Erv29"
/locus_tag="P301_G13661"
/note="Similar to YGR284C Protein localized to
COPII-coated vesicles, involved in vesicle formation and
incorporation of specific secretory cargo"
/codon_start=1
/product="Erv29p"
/protein_id="EWG91213.1"
/translation="MSYRGPTGNFGGMPMSSSQGPYSGGAQFRSNQNQSTSGILKQWK
HSFEKFASRIEGLTDNAVVYKLKPYIPSLSRFFIVATFYEDSFRILSQWSDQIFYLNK
WKHYPYFFVVVFLVVVTVSMLIGASLLVLRKQTNYATGVLCACVISQALVYGLFTGSS
FVLRNFSVIGGLLIAFSDSIVQNKTTFGMLPELNSKNDKAKGYLLFAGRILIVLMFIA
FTFSKSWFTVVLTIIGTICFAIGYKTKFASIMLGLILTFYNITLNNYWFYNNTKRDFL
KYEFYQNLSIIGGLLLVTNTGAGELSVDEKKKIY"
gene complement(1023449..1024750)
/gene="Zuo1"
/locus_tag="P301_G13666"
mRNA complement(1023449..1024750)
/gene="Zuo1"
/locus_tag="P301_G13666"
/product="Zuo1p"
CDS complement(1023449..1024750)
/gene="Zuo1"
/locus_tag="P301_G13666"
/note="Similar to YGR285C 'Ribosome-associated chaperone,
functions in ribosome biogenesis and, in partnership with
Ssz1p and SSb1/2, as a chaperone for nascent polypeptide
chains; contains a DnaJ domain and functions as a
J-protein partner for Ssb1p and Ssb2p'"
/codon_start=1
/product="Zuo1p"
/protein_id="EWG91214.1"
/translation="MFSLPTLTSDITVEVNSSATKTPFVRRPVEPVGKFFLQHAQRTL
RNHTWSEFERIEAEKNVKTVDESNVDPDELLFDTELADEDLLTHDARDWKTADLYAAM
GLSKLRFRATESQIIKAHRKQVVKYHPDKQSAAGGSLDQDGFFKIIQKAFETLTDSNK
RAQYDSCDFVADVPPPKKGTDYDFYEAWGPVFEAEARFSKKTPIPSLGNKDSSKKEVE
QFYAFWHRFDSWRTFEFLDEDVPDDSSNRDHKRYIERKNKAARDKKKTADNARLVKLV
ERAVSEDPRIKMFKEEEKKEKERRKWEREAGARAEAEAKAKAEAEAKAKAESEAKANA
SAKADKKKAKEAAKAAKKKNKRAIRNSAKEADYFGDADKAATIDEQVGLIVDSLNDEE
LVSTADKIKANAAGAKEVLKESAKTIVDSGKLPSSLLSYFV"
gene complement(1025412..1026539)
/gene="Bio2"
/locus_tag="P301_G13671"
mRNA complement(1025412..1026539)
/gene="Bio2"
/locus_tag="P301_G13671"
/product="Bio2p"
CDS complement(1025412..1026539)
/gene="Bio2"
/locus_tag="P301_G13671"
/note="Similar to YGR286C 'Biotin synthase, catalyzes the
conversion of dethiobiotin to biotin, which is the last
step of the biotin biosynthesis pathway; complements E.
coli bioB mutant'"
/codon_start=1
/product="Bio2p"
/protein_id="EWG91215.1"
/translation="MMSTIYRHLSTARPALTKYATNAAVKSTTASSEASTLGALQYAL
SLDEPSHSWTKSQLKEIYHTPLLELTHAAQLQHRKWHDPTKVQLCTLMNIKSGGCSED
CKYCAQSSRNDTGLKAEKMVKVDEVIKEAEEAKRNGSTRFCLGAAWRDMKGRKSAMKR
IQEMVTKVNDMGLETCVTLGMVDQDQAKQLKDAGLTAYNHNIDTSREHYSKVITTRTY
DDRLQTIKNVQESGIKACTGGILGLGESEDDHIGFIYTLSNMSPHPESLPINRLVAIK
GTPMAEELADPKSKKLQFDEILRTIATARIVMPKAIIRLAAGRYTMKETEQFVCFMAG
CNSIFTGKKMLTTMCNGWDEDKAMLAKWGLQPMEAFKYDRS"
gene complement(1028818..1030587)
/locus_tag="P301_G13676"
mRNA complement(1028818..1030587)
/locus_tag="P301_G13676"
/product="hypothetical protein"
CDS complement(1028818..1030587)
/locus_tag="P301_G13676"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG91216.1"
/translation="MTISSAHPETEPKWWKEATFYQIYPASFKDSNDDGWGDMKGISS
KLEYIKELGVDAIWISPFYDSPQDDMGYDIANYEKVWPTYGTNEDCFALIEKTHKLGM
KFITDLVINHCSSEHEWFKESRSSKTNPKRDWFFWRPPKGYDAEGKPIPPNNWKSYFG
GSAWTFDEKTQEFYLRLFCSTQPDLNWENEDCRKAIYESAVGYWLDHGVDGFRIDVGS
LYSKVVGLPDAPVVDKNSTWQSSDPYTLNGPRIHEFHQEMNQFIRNRVKDGREIMTVG
EMQHASDETKRLYTSASRHELSELFNFSHTDVGTSPLFRYNLVPFELKDWKIALAELF
RYINGTDCWSTIYLENHDQPRSITRFGDDSPKNRVVSGKLLSVLLSALTGTLYGYQGQ
ELGQINFKNWPVEKYEDVEIRNNYNAIKEEHGENSEEMKKFLEAIALISRDHARTPMQ
WSREEPNAGFSGPSAKPWFYLNDSFREGINVEDEIKDPNSVLNFWKEALKFRKAHKDI
TVYGYDFEFIDLDNKKLFSFTKKYNNKTLFAALNFSSDATDFKIPNDDSSFKLEFGNY
PKKEVDASSRTLKPWEGRIYISE"
gene 1031900..1033321
/gene="Mal13"
/locus_tag="P301_G13681"
mRNA 1031900..1033321
/gene="Mal13"
/locus_tag="P301_G13681"
/product="Mal13p"
CDS 1031900..1033321
/gene="Mal13"
/locus_tag="P301_G13681"
/note="Similar to YGR288W 'MAL-activator protein, part of
complex locus MAL1; nonfunctional in genomic reference
strain S288C'"
/codon_start=1
/product="Mal13p"
/protein_id="EWG91217.1"
/translation="MTLTKQTCAKQACDCCRIRRVKCDGKRPCSSCLQNSLDCTYLQP
SRKRGPKSIRLRSLKRIAEVQRESGPNTIATAPVIYKRVPKKLIDECLRLYHDNLYVI
WPLLSYDDLHKLLEEKYNDNYVYWFLTALSAATLSDLQTEIKSEEEVTFTGKQLSNLC
ISPCQQFDDLDNSNIFNIMTYYCLHRSFAQISNARTSYRLCCEAVGLITVAGLHREET
YGSLTFEEQQLRRKLYYLLLMTERYYAIYLHCATSLDATIAPPQLELVTDPQLSMDSF
LEMIRIFTVPGKCFFDALAADSTDASCTEESLKKIWNELHTTSSEIEPWSNGYIDISF
SRRWIRILAWKLAYQMRGSNFSLNANNGQIPIEIARDMLIDTYLTPENLYDVHGPGVP
VKTLEIATALVDIVGQYDHNMKLEAWNVLHDVCKFAFSLNHYNNDMLKRFSTKCQNAL
ITLPISKPLQLDGYPKDNEDIDP"
assembly_gap 1035009..1035027
/estimated_length=19
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(1035366..1037216)
/gene="Mal11"
/locus_tag="P301_G13686"
/note="nonfunctional due to frameshift determined by
sequencing error"
CONTIG join(APIQ01000266.1:1..9492,gap(19),APIQ01000267.1:1..50935,
gap(28),APIQ01000268.1:1..3747,gap(212),APIQ01000269.1:1..8446,
gap(250),APIQ01000270.1:1..6441,gap(16),APIQ01000271.1:1..4811,
gap(18),APIQ01000272.1:1..4139,gap(167),APIQ01000273.1:1..20732,
gap(831),APIQ01000274.1:1..3974,gap(100),APIQ01000275.1:1..11511,
gap(73),APIQ01000276.1:1..13108,gap(170),APIQ01000277.1:1..4811,
gap(340),APIQ01000278.1:1..10963,gap(20),APIQ01000279.1:1..9600,
gap(14),APIQ01000280.1:1..3050,gap(15),APIQ01000281.1:1..34811,
gap(127),APIQ01000282.1:1..1992,gap(48),APIQ01000283.1:1..6099,
gap(119),APIQ01000284.1:1..17625,gap(26),APIQ01000285.1:1..25751,
gap(300),APIQ01000286.1:1..7597,gap(35),APIQ01000287.1:1..9363,
gap(106),APIQ01000288.1:1..11313,gap(450),APIQ01000289.1:1..20704,
gap(115),APIQ01000290.1:1..11260,gap(1230),APIQ01000291.1:1..3532,
gap(843),APIQ01000292.1:1..2207,gap(64),APIQ01000293.1:1..1693,
gap(102),APIQ01000294.1:1..28811,gap(75),APIQ01000295.1:1..990,
gap(133),APIQ01000296.1:1..20535,gap(34),APIQ01000297.1:1..9088,
gap(33),APIQ01000298.1:1..6745,gap(135),APIQ01000299.1:1..5223,
gap(379),APIQ01000300.1:1..1296,gap(631),APIQ01000301.1:1..22806,
gap(328),APIQ01000302.1:1..1687,gap(333),APIQ01000303.1:1..707,
gap(236),APIQ01000304.1:1..4774,gap(54),APIQ01000305.1:1..26352,
gap(79),APIQ01000306.1:1..6109,gap(174),APIQ01000307.1:1..10368,
gap(306),APIQ01000308.1:1..5777,gap(50),APIQ01000309.1:1..11234,
gap(421),APIQ01000310.1:1..3897,gap(22),APIQ01000311.1:1..29963,
gap(904),APIQ01000312.1:1..2230,gap(276),APIQ01000313.1:1..3822,
gap(191),APIQ01000314.1:1..10236,gap(56),APIQ01000315.1:1..797,
gap(160),APIQ01000316.1:1..835,gap(23),APIQ01000317.1:1..4751,
gap(1687),APIQ01000318.1:1..17137,gap(91),APIQ01000319.1:1..13529,
gap(67),APIQ01000320.1:1..5982,gap(426),APIQ01000321.1:1..15187,
gap(536),APIQ01000322.1:1..11682,gap(432),APIQ01000323.1:1..20026,
gap(265),APIQ01000324.1:1..1336,gap(102),APIQ01000325.1:1..1627,
gap(169),APIQ01000326.1:1..11964,gap(62),APIQ01000327.1:1..6840,
gap(248),APIQ01000328.1:1..4002,gap(83),APIQ01000329.1:1..20734,
gap(358),APIQ01000330.1:1..16136,gap(92),APIQ01000331.1:1..3679,
gap(166),APIQ01000332.1:1..2047,gap(1384),APIQ01000333.1:1..5090,
gap(519),APIQ01000334.1:1..2260,gap(97),APIQ01000335.1:1..3069,
gap(20),APIQ01000336.1:1..2623,gap(74),APIQ01000337.1:1..8858,
gap(197),APIQ01000338.1:1..8842,gap(115),APIQ01000339.1:1..7817,
gap(29),APIQ01000340.1:1..4165,gap(99),APIQ01000341.1:1..917,
gap(576),APIQ01000342.1:1..1926,gap(301),APIQ01000343.1:1..5411,
gap(385),APIQ01000344.1:1..4714,gap(84),APIQ01000345.1:1..16214,
gap(151),APIQ01000346.1:1..12146,gap(15),APIQ01000347.1:1..8427,
gap(38),APIQ01000348.1:1..3093,gap(77),APIQ01000349.1:1..2540,
gap(117),APIQ01000350.1:1..1593,gap(190),APIQ01000351.1:1..1389,
gap(19),APIQ01000352.1:1..29073,gap(216),APIQ01000353.1:1..3067,
gap(129),APIQ01000354.1:1..15009,gap(24),APIQ01000355.1:1..9100,
gap(92),APIQ01000356.1:1..3422,gap(163),APIQ01000357.1:1..1012,
gap(171),APIQ01000358.1:1..17167,gap(42),APIQ01000359.1:1..2704,
gap(435),APIQ01000360.1:1..17159,gap(62),APIQ01000361.1:1..8169,
gap(76),APIQ01000362.1:1..8532,gap(22),APIQ01000363.1:1..7925,
gap(33),APIQ01000364.1:1..16862,gap(56),APIQ01000365.1:1..8514,
gap(359),APIQ01000366.1:1..38296,gap(78),APIQ01000367.1:1..35155,
gap(19),APIQ01000368.1:1..6824)
//