LOCUS KK037038 999820 bp DNA linear CON 11-FEB-2014
DEFINITION Saccharomyces cerevisiae R008 chromosome XII genomic scaffold
scaffold18, whole genome shotgun sequence.
ACCESSION KK037038 APIP01000000
VERSION KK037038.1
DBLINK BioProject: PRJNA162713
BioSample: SAMN01915092
KEYWORDS WGS.
SOURCE Saccharomyces cerevisiae R008
ORGANISM Saccharomyces cerevisiae R008
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 999820)
AUTHORS Treu,L., Toniolo,C., Nadai,C., Sardu,A., Giacomini,A., Corich,V.
and Campanaro,S.
TITLE The impact of genomic variability on gene expression in
environmental Saccharomyces cerevisiae strains
JOURNAL Environ. Microbiol. (2013) In press
PUBMED 24238297
REMARK Publication Status: Available-Online prior to print
REFERENCE 2 (bases 1 to 999820)
AUTHORS Campanaro,S., Treu,L. and Corich,V.
TITLE Direct Submission
JOURNAL Submitted (25-FEB-2013) Department of Biology, University of Padua,
Via U. Bassi 58/b, Padova, PD 35121, Italia
COMMENT ##Genome-Assembly-Data-START##
Assembly Method :: Newbler v. 2.3
Assembly Name :: R008
Genome Coverage :: 14.0x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..999820
/organism="Saccharomyces cerevisiae R008"
/mol_type="genomic DNA"
/submitter_seqid="scaffold18"
/strain="R008"
/isolation_source="grape bunch from Raboso Piave vineyard"
/db_xref="taxon:1182966"
/chromosome="XII"
/country="Italy: Tezze di Piave (TV)"
/collection_date="Oct-2005"
gene complement(799..1773)
/gene="Mht1"
/locus_tag="R008_L10001"
mRNA complement(799..1773)
/gene="Mht1"
/locus_tag="R008_L10001"
/product="Mht1p"
CDS complement(799..1773)
/gene="Mht1"
/locus_tag="R008_L10001"
/note="Similar to YLL062C S-methylmethionine-homocysteine
methyltransferase, functions along with Sam4p in the
conversion of S-adenosylmethionine (AdoMet) to methionine
to control the methionine/AdoMet ratio"
/codon_start=1
/product="Mht1p"
/protein_id="EWG84231.1"
/translation="MKRIPIKELIVEHPGKVLILDGGQGTELENRGININSPVWSAAP
FTSESFWEPSSQERKVVEEMYRDFMIAGANILMTITYQANFQSISENTSIKTLAAYKR
FLDKIVSFTREFIGEKRYLIGSIGPWAAHVSCEYTGDYGPHPENIDYYGFFKPQLENF
NQNRDIDLIGFETIPNFHELKAILSWDEDIISKPFYIGLSVDDNSLLRDGTTLEEISV
HIKGLGNKINKNLLLMGVNCVSFNQSALILKMLHEHLPGMPLLVYPNSGEIYNPKEKT
WHRPTNKLDDWETMVKKFVDNGARIIGGCCRTSPKDIAEIASAVDKYS"
gene 2116..3873
/gene="Mmp1"
/locus_tag="R008_L10006"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 3898..3930
/estimated_length=33
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(5288..5947)
/gene="Gtt2"
/locus_tag="R008_L10026"
mRNA complement(5288..5947)
/gene="Gtt2"
/locus_tag="R008_L10026"
/product="Gtt2p"
CDS complement(5288..5947)
/gene="Gtt2"
/locus_tag="R008_L10026"
/note="Similar to YLL060C 'Glutathione S-transferase
capable of homodimerization; functional overlap with
Gtt2p, Grx1p, and Grx2p'"
/codon_start=1
/product="Gtt2p"
/protein_id="EWG84232.1"
/translation="MKQKMIIYDTPAGPYPARVRIALAEKNMLSSVQFVRINLWKGEH
KKPEFLAKNYSGTVPVLELDDGTLIAECTAITEYIDALDGTPTLTGKTPLEKGVIHMM
NKRAELELLDPVSVYFHHATPGLGPEVEFYQNKEWGLRQRGKALHGMHYFDTVLRERP
YVAGDSFSMADITVIAGLIFAAIVKLQVPEECEALRAWYKRMQQRPSVKKLLEIRSKS
S"
gene 7711..9438
/locus_tag="R008_L10041"
mRNA 7711..9438
/locus_tag="R008_L10041"
/product="hypothetical protein"
CDS 7711..9438
/locus_tag="R008_L10041"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84233.1"
/translation="MTEIEFGQPLPSNLDYAVSFGIPTWDSAIGYAEKVPEVIGKMAT
GYPRYFPQPPVQRLCAYFVKKFGRGSENCRPFPSVNLGLNCLEYVKSVKGPESEAHLE
VETVTIKNRGAKTSKEPAELVLTIAAVLASEEEFEIVKEYWKLRGECVSSRLALSVNQ
LLDCANHGSEQVLRELEAGVFAAKKGEEKAKNLIKGRIVENRFKPFGLEKKTPNWEGL
NLNPNEDVYLVSSGMSAISTARNLLTFWEEKKNLGDSLNKTTSDQKKKPLLCDTVGIF
GFPFKDTQVIMTKFGKCKFFGFGNSRDVVELQKFLETSKQRILAVFVETPSNPLLNMP
DLKKLRRLADQYGFFIVIDDTIGGLNVDILPYADIVSTSLTKLFNGASNVMGGSVVLN
PKSSLYPYAREYFRSANFEDLLWCEDAIVLERNSRDFEDRTLRANANTEKLLNDLLLP
EEEKICKKIYYPTVTSKETFENYESVRNERGGYGCLFSVAFFNEGDAKAFYDSLKVFK
GPSNGTNFTLACPYVHLAHHSELEEVSKFGADPNIIRVSVGLEDIQWLLKVFSSALDV
VKSRGSKHS"
gene complement(9896..11134)
/gene="Jlp1"
/locus_tag="R008_L10046"
mRNA complement(9896..11134)
/gene="Jlp1"
/locus_tag="R008_L10046"
/product="Jlp1p"
CDS complement(9896..11134)
/gene="Jlp1"
/locus_tag="R008_L10046"
/note="Similar to YLL057C 'Fe(II)-dependent
sulfonate/alpha-ketoglutarate dioxygenase, involved in
sulfonate catabolism for use as a sulfur source; contains
sequence that resembles a J domain (typified by the E.
coli DnaJ protein); induced by sulphur starvation'"
/codon_start=1
/product="Jlp1p"
/protein_id="EWG84234.1"
/translation="MSPAAAQTAIPLPSTDLPVKIITNGLKNLNYTSKQGYGNFDTHF
YDGQDEVSPSGLLKIRKSYREKSKYPDYLPTWDPTEKYGPLEFHEYHDPALRADGNFS
NLFAKENVGQLKVKKITPKLGLEINGIQLTDLSDAAKDELALLVAQKGVVVFRNQNFA
DEGPDYVTEYGRHFGKLHIHQTSGHPQNNPELHITFRRPDAEEFARVFDDSTSSGGWH
TDVSYELQPPSYTFFSVVEGPDGGGDTLFADTIEAFDRLSKPLQDFLSTLHVIHSSKE
QAENSQRQGGIKRRAPVTHIHPLVRVHPVLKKKCLYVNRAFSRKIVELKRQESESLLN
FLYNLVESSHDLQLRAKWEPHSVVIWDNRRVQHSAVIDWEEPIHRHAFRITPQAERPV
EDLKFLNDENYYPSSLTLDI"
gene complement(11549..12445)
/locus_tag="R008_L10056"
mRNA complement(11549..12445)
/locus_tag="R008_L10056"
/product="hypothetical protein"
CDS complement(11549..12445)
/locus_tag="R008_L10056"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84235.1"
/translation="MKVFVTGASGFIGSAVLSELISSGHEVVGLARSDEAAAKIKSIN
PAAKILRGDLKDLEILKKGATESDGVIHLGFVHDFKNFEQCCEIDRQATVAMLESLKG
SNKPFLYTNGTLSLRPNKVANEQDGIDEDSKILRAVTEQVALSYKDKGVSARIVRLPF
SVHGKGDKAFVPILMNIAKAAGKSGYVGQGTNAWAAVHRLDTAPLFRLVLEKGKTGQV
YHCVGEQGIPFKDIARVIGEILNVPVASIPVDDAESHFGFLTCFVTRDGPVSSEGTRK
ELGWQPQQIGLLEDIRANYSLN"
gene 14249..15844
/gene="Yct1"
/locus_tag="R008_L10061"
mRNA 14249..15844
/gene="Yct1"
/locus_tag="R008_L10061"
/product="Yct1p"
CDS 14249..15844
/gene="Yct1"
/locus_tag="R008_L10061"
/note="Similar to YLL055W 'High-affinity cysteine-specific
transporter with similarity to the Dal5p family of
transporters; green fluorescent protein (GFP)-fusion
protein localizes to the endoplasmic reticulum; YCT1 is
not an essential gene'"
/codon_start=1
/product="Yct1p"
/protein_id="EWG84236.1"
/translation="MSKVDVKIGADSISSSDEILVPSRLADVTLAFMEENDAAVPEIT
PEQEKKLKRKLFLTIFTFVSAINLLLYMDKATLSYDSILGFFEDTGLTQNTYNTVNTL
FYVGFAIGQFPGQYLAQKLPLGKFLGGLLATWTILIFLSCTAYNFSGVVALRFFLGLT
ESVVIPILITTMGMFFDASERAAAQPFFFAACMGSPIPTGFIAYGVLHITNPSISLWK
IFTIIIGGLTFIMTVVVILWFPNNPADVKFFSIQERVWIIRRVQASTGSSIEQKVFKK
SQFREAMKDYITWLFGLFFLLQQLANNLPYQQNLLFEGMGGVDALGSTLVSVAGAGFA
VVCAFIATLMLAKWKNISALTAIFWTLPALVGSIAAAALPWDNKIGILANICMAGQIF
GIPFIIALSWASSSASGYTKKLTRSSVSLFAMGIANIISPQIWREKDSPRFLPAWIVQ
IVLSFSLAPAILLLIHFILKRRNNQRLKNYEENLQNYLDRIQLIESENPSSIEEGKVV
THENNLAVFDLTDLENETFIYPL"
gene complement(16812..19343)
/locus_tag="R008_L10066"
mRNA complement(16812..19343)
/locus_tag="R008_L10066"
/product="hypothetical protein"
CDS complement(16812..19343)
/locus_tag="R008_L10066"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84237.1"
/translation="MSIASQKKVKPSFVCLRCKQRKIKCDKLWPTCSKCKASSSICSY
EVEPGRINKSPIIENAPHRDIRNITPASMSASGSFTSILNPSTKDWEMKNFAMNLSNA
HDKLVVMNNTTIVDSPFAFHSILQHDLYAKALTTCIHERILIDVERHRENVSANNKKR
ELNLTIGDIGPLFFIDKAALKFIENTSKTSKLYPPIDFLYNTYDYEQAHPEENNDKIS
INILLEELSKYFLNKNEVDGLIVDFYKTIYPVYPLLEISLFEDNIRELLQLNEFNGYN
IVFAGKDSRRKLETITLLTIILAFSYRRLSLSTSHSFKESFGVKSNNLTLLAHKLLAL
MNVFQYVNEHTLCCLLYFFILRYLNPDQADMYPTHSDILNLKFLENVAIKLGLNEEPF
QYTRYVSESDDYPRLFNLRRKLWLGVQFLKFGILIPEGDSDILSLEYLRSFMKTDESL
PELFERNYASTNNLDLSLMATAENIYHLHLSLQVLLTSCFPINGPSYLKEVLDNIDKT
KDFLNQKFPLILSSLGEPRMKSLHINVPSSLANEESFDFSTFEENETFIANVISYTCT
MNIYDSLSLHFENQCFKNALEYKTYYHRFTFTAIQDYLTLLKLISEYFNGSLLHLREP
FGFATQKVVRFSIHRLLIFQATLLVRLFYKKDTCDRSSAAMGMLNDRNGRLSRVIEKM
IKLMSYHMKLLVEIVTSKLEKSYLGSFISVSIFRYIIYLVDTDALSAFISDYWKSDAV
MDERYSRIHRIVGLKWGMGRDKSFSFTSKLNNPQFLGSLDLEILEELEKLISAQEFSR
NFTEDVDESLQSEIDLMNYDNEALNQLMAIDLDKLLGIFPNLSNF"
gene complement(19641..20099)
/locus_tag="R008_L10076"
mRNA complement(19641..20099)
/locus_tag="R008_L10076"
/product="hypothetical protein"
CDS complement(19641..20099)
/locus_tag="R008_L10076"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84238.1"
/translation="MWFPQIIAGMAAGGAASAMTPGKVLFTNALGLGCSRSRGLFLEM
FGTAVLCLTVLMTAVEKRETNFMAALPIGISLFMAHMALTGYTGTGVNPARSLGAAVA
ARYFPHYHWIYWISPLLGAFLAWSVWQLLQILDYTTYVNAEKAAGQKKED"
gene complement(20050..20499)
/gene="Aqy2"
/locus_tag="R008_L10081"
mRNA complement(20050..20499)
/gene="Aqy2"
/locus_tag="R008_L10081"
/product="Aqy2p"
CDS complement(20050..20499)
/gene="Aqy2"
/locus_tag="R008_L10081"
/note="Similar to YLL052C 'Water channel that mediates the
transport of water across cell membranes, only expressed
in proliferating cells, controlled by osmotic signals, may
be involved in freeze tolerance; disrupted by a stop codon
in many S. cerevisiae strains'"
/codon_start=1
/product="Aqy2p"
/protein_id="EWG84239.1"
/translation="MSNESNDLEKNISHLDPTGVDNAYIPPEQPETKHSRFNIDRDTL
RNHFIAAVGEFCGTFMFLWCAYVICNVANHDVALITEPEGSHPGQLIMIALGFGFSVM
FSIWCFWWGFEPSRFSLFVFGQSHLTSQMCSDVVSSDHCWDGCWWCR"
gene complement(21471..23609)
/gene="Fre6"
/locus_tag="R008_L10091"
mRNA complement(21471..23609)
/gene="Fre6"
/locus_tag="R008_L10091"
/product="Fre6p"
CDS complement(21471..23609)
/gene="Fre6"
/locus_tag="R008_L10091"
/note="Similar to YLL051C 'Putative ferric reductase with
similarity to Fre2p; expression induced by low iron
levels'"
/codon_start=1
/product="Fre6p"
/protein_id="EWG84240.1"
/translation="MHRTLLFLTWLISLTKAFNIKLPHTEKKDHLESNAVLACASYIN
TLKWSFDSSVVPGFYSTICSYSPAFDTWSLCIFNSLTDQIIPMDNTSFEESLGNVRKT
CSFVDKKFSNISLEQYYSSLNNASSHALEDYGSIESLSTSIRVDRETRSRWIRAFHAH
AYNLDISSVYGAYLTYYFVIVGIIAVFFHMSHYNGLNRALFASRFVNYIRGHFVLPTF
LVDKHANHFKFLNVEVFTGLMPNSLEAWIIFGYTLANIIFLSISYIIDPYNLIFNSHL
SQFTRLLADRSGILAFTQFPLIIIFTARNSFLEFLTGVKFNSFISFHKWIGRIMVLNA
TIHSLSYSLFAIINHAFKISNKQLYWKFGIASITVLCVLLVLSLGIVRKRHYEFFLYT
HIILALLFFYCCWQHVKIFNGWKEWIVVSLLIWGLEKLFRIWNILQFRFPKATLINLN
TSNNPHDEMFKVIIPKYNRRWHSKPGQYCFIYFLHPLVFWQCHPFTIIDEGEKCVLVI
KPKSGLTRFIYNHILQSLNGKLQLRVAIEGPYGPSNLHLDKFDHLLLLSGGTGLPGPL
DHAIKLSRNPDKPKSIDLIMAIKNPSFLNGYKSEILELKNSRSHVNVQVYLTQKTAVT
KAANARDQLIHFDDIMTELTSFAHIGNARPYFSNVIENAIKSTPPGDSLAVVCCGPPV
LVDDVRNTVSQKLLGYPERIIEYFEEYQCW"
gene complement(23942..24552)
/gene="Cof1"
/locus_tag="R008_L10096"
mRNA complement(join(23942..24359,24539..24552))
/gene="Cof1"
/locus_tag="R008_L10096"
/product="Cof1p"
CDS complement(join(23942..24359,24539..24552))
/gene="Cof1"
/locus_tag="R008_L10096"
/note="Similar to YLL050C 'Cofilin, promotes actin
filament depolarization in a pH-dependent manner; binds
both actin monomers and filaments and severs filaments;
thought to be regulated by phosphorylation at SER4;
ubiquitous and essential in eukaryotes'"
/codon_start=1
/product="Cof1p"
/protein_id="EWG84241.1"
/translation="MSRSGVAVADESLTAFNDLKLGKKYKFILFGLNDAKTEIVVKET
STDPSYDAFLEKLPENDCLYAIYDFEYEINGNEGKRSKIVFFTWSPDTAPVRSKMVYA
SSKDALRRALNGVSTDVQGTDFSEVSYDSVLERVSRGAGSH"
gene 24804..25346
/gene="Ldb18"
/locus_tag="R008_L10101"
mRNA 24804..25346
/gene="Ldb18"
/locus_tag="R008_L10101"
/product="Ldb18p"
CDS 24804..25346
/gene="Ldb18"
/locus_tag="R008_L10101"
/note="Similar to YLL049W 'Component of the dynactin
complex, which is required for dynein activity; null
mutant exhibits defects in nuclear migration and spindle
orientation and has reduced affinity for alcian blue dye;
has homology to mammalian dynactin subunit p24'"
/codon_start=1
/product="Ldb18p"
/protein_id="EWG84242.1"
/translation="MPGLKLVEALEYRCDRLERLIGAGYSANSDVSVQLDELYNQLHR
LYFQGLKYSQDLLQLFNTFMAEDIENVGAPDDICIFASCFDDIYTLYSAFDELNSQYM
EFCQISKSSLDQISFKDANIETKQLKKLPELVDNCNIMILRSIAILNRFIDWNIEVNG
FFQFQKKRLLEAVEFTKGNL"
gene complement(25428..30423)
/gene="Ybt1"
/locus_tag="R008_L10106"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(30855..31621)
/gene="Rnp1"
/locus_tag="R008_L10116"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(32027..32797)
/gene="Rpl8b"
/locus_tag="R008_L10121"
mRNA complement(32027..32797)
/gene="Rpl8b"
/locus_tag="R008_L10121"
/product="Rpl8bp"
CDS complement(32027..32797)
/gene="Rpl8b"
/locus_tag="R008_L10121"
/note="Similar to YLL045C 'Ribosomal protein L4 of the
large (60S) ribosomal subunit, nearly identical to Rpl8Ap
and has similarity to rat L7a ribosomal protein; mutation
results in decreased amounts of free 60S subunits'"
/codon_start=1
/product="Rpl8bp"
/protein_id="EWG84243.1"
/translation="MAPGKKVAPAPFGAKSTKSNKAKNPLTHSTPKNFGIGQAVQPKR
NLSRYVKWPEYVRLQRQKKILSIRLKVPPTIAQFQYTLDRNTAAETFKLFNKYRPETA
AEKKERLTKEAAAIAEGKSKQDASPKPYAVKYGLNHVVSLIENKKAKLVLIANDVDPI
ELVVFLPALCKKMGVPYAIIKGKARLGTLVNQKTSAVAALTEVRAEDEAALAKLVSTI
DANFADKYDEVKKHWGGGILGNKAQAKMDKRAKTSDSA"
assembly_gap 32981..32997
/estimated_length=17
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 34116..36125
/gene="Fps1"
/locus_tag="R008_L10136"
mRNA 34116..36125
/gene="Fps1"
/locus_tag="R008_L10136"
/product="Fps1p"
CDS 34116..36125
/gene="Fps1"
/locus_tag="R008_L10136"
/note="Similar to YLL043W 'Plasma membrane channel, member
of major intrinsic protein (MIP) family; involved in
efflux of glycerol and in uptake of acetic acid and the
trivalent metalloids arsenite and antimonite;
phosphorylated by Hog1p MAPK under acetate stress'"
/codon_start=1
/product="Fps1p"
/protein_id="EWG84244.1"
/translation="MSNPQKALNDFLSSESVHTHDSSRKQSNKQSSDEGRSSSQPSHH
HSGGTNNNNNNNNNNNNSNNNNNGNDGGNDDDYDYEMQDYRPSPQSARPTPTYVPQYS
VESGTAFPIQEVIPSAYINTQDINHKDNGPPSASSNRAFRPRGQTTVSANVLNIEDFY
KNADDAHTIPESHLSRRRSRSRATSNAGHSANTGATNGRTTGAQTNMESNESPRNVPI
MVKPKTLYQNPQTPTVLPSTYHPINKWSSVKNTYLKEFLAEFMGTMVMIIFGSAVVCQ
VNVAGKIQQDNFNVALDNLNVTGSSAETIDAMKSLTSLVSSVAGGTFDDVALGWAAAV
VMGYFCAGGSAISGAHLNPSITLANLVYRGFPLKKVPYYFAGQLIGAFTGALILFIWY
KRVLQEAYSDWWMNESVAGMFCVFPKPYLSSGRQFFSEFLCGAMLQAGTFALTDPYTC
LSSDVFPLMMFILIFIINASMAYQTGTAMNLARDLGPRLALYAVGFDHKMLWVHHHHF
FWVPMVGPFIGALMGGLVYDVCIYQGHESPVNWSLPVYKEMIMRAWFRRPGWKKRNRA
RRTSDLSDFSYNNDDDEEFGERMALQKTKTKSSISDNENEAGEKKVQFKSVQRGKRTF
GGIPTILEEEDSIETASLGATTTDSIGLSDTSSEDSHYGNAKKVT"
gene complement(36266..36769)
/gene="Atg10"
/locus_tag="R008_L10141"
mRNA complement(36266..36769)
/gene="Atg10"
/locus_tag="R008_L10141"
/product="Atg10p"
CDS complement(36266..36769)
/gene="Atg10"
/locus_tag="R008_L10141"
/note="Similar to YLL042C Conserved E2-like conjugating
enzyme that mediates formation of the Atg12p-Atg5p
conjugate, which is a critical step in autophagy"
/codon_start=1
/product="Atg10p"
/protein_id="EWG84245.1"
/translation="MIPYQEWHSQLQSLYDSQIFHNWALCQDVHLNDEKDGLLLRLIP
TRQLQKNTERIENKLLNHIELYLTYSKVYNEPLLLLRIWEEKSIDGIPMTKLMLPTDI
ESLLDVQGKFQLGLDTIINLEGSVWYSFHPCDTSCIVGDQAEFMSTYLRRWVSIFIFS
WLGYEDS"
gene complement(37308..38108)
/gene="Sdh2"
/locus_tag="R008_L10146"
mRNA complement(37308..38108)
/gene="Sdh2"
/locus_tag="R008_L10146"
/product="Sdh2p"
CDS complement(37308..38108)
/gene="Sdh2"
/locus_tag="R008_L10146"
/note="Similar to YLL041C Iron-sulfur protein subunit of
succinate dehydrogenase (Sdh1p, Sdh2p, Sdh3p, Sdh4p),
which couples the oxidation of succinate to the transfer
of electrons to ubiquinone as part of the TCA cycle and
the mitochondrial respiratory chain"
/codon_start=1
/product="Sdh2p"
/protein_id="EWG84246.1"
/translation="MLNVLLRRKAFCLVTKKGMATATTAAATHTPRLKTFKVYRWNPD
EPSAKPHLQSYQVDLNDCGPMVLDALLKIKDEQDSTLTFRRSCREGICGSCAMNIGGR
NTLACICKIDQNESKQLKIYPLPHMFIVKDLVPDLTNFYQQYKSIQPYLQRSSFPKDG
TEVLQSIEDRKKLDGLYECILCACCSTSCPSYWWNQEQYLGPAVLLQAYRWLIDSRDQ
ATKTRKAMLNNSMSLYRCHTIMNCTRTCPKGLNPGLAIAEIKKSLAFA"
gene complement(38388..47822)
/gene="Vps13"
/locus_tag="R008_L10151"
mRNA complement(38388..47822)
/gene="Vps13"
/locus_tag="R008_L10151"
/product="Vps13p"
CDS complement(38388..47822)
/gene="Vps13"
/locus_tag="R008_L10151"
/note="Similar to YLL040C 'Protein of unknown function;
heterooligomeric or homooligomeric complex; peripherally
associated with membranes; homologous to human COH1;
involved in sporulation, vacuolar protein sorting and
protein-Golgi retention'"
/codon_start=1
/product="Vps13p"
/protein_id="EWG84247.1"
/translation="MLESLAANLLNRLLGSYVENFDPNQLNVGIWSGDVKLKNLKLRK
DCLDSLNLPIDVKSGILGDLVLTVPWSSLKNKPVKIIIEDCYLLCSPRSEDHENDEEM
IKRAFRLKMRKVSEWELTNQARILSTQSENKTSSSSSEKNNAGFMQSLTTKIIDNLQV
TIKNIHLRYEDMDGIFTTGPSSVGLTLNELSAVSTDSNWAPSFIDITQNITHKLLTLN
SLCLYWNTDSPPLISDDDQDRSLENFVRGFKDMIASKNSTAPKHQYILKPVSGLGKLS
INKLGSTEEQPHIDLQMFYDEFGLELDDTEYNDILHVLSSIQLRQITKKFKKARPSFA
VSENPTEWFKYIAACVINEIHEKNKMWTWESMKEKCEQRRLYTKLWVEKLKLKNLEAP
LRDPIQEAQLSELHKDLTYDEIILFRSVAKRQYAQYKLGMTEDSPTPTASSNIEPQTS
NKSATKNNGSWLSSWWNGKPTEEVDEDLIMTEEQRQELYDAIEFDENEDKGPVLQVPR
ERVELRVTSLLKKGSFTIRKKKQNLNLGSIIFENCKVDFAQRPDSFLSSFQLNKFSLE
DGLPNALYKHIISVRNSSKDQSSIDNHATGEEEEEDEPLLRASFELNPLDGLADSNLN
IKLLGMTVFYHVHFITEVHKFFKASNQHMETIGNIVNAAEATVEGWTTQTRMGIESLL
EDHKTVNVSLDLQAPLIILPLDPHDWDTPCAIIDAGHMSILSDLVPKEKIKEIKELSP
EEYDKIDGNEINRLMFDRFQILSQDTQIFVGPDIQSTIGKINTASSTNDFRILDKMKL
ELTVDLSILPKAYKLPTIRVFGHLPRLSLSINDIQYKTIMNLIANSIPSMIDDEENNG
DYVNYSSGSEKEMKKQIQLQLKNTLKALENMQPLQIEQKFLELHFDIDQAKIAFFQCI
KNDSRNSEKLVDILCQRLNFNFDKRAKEMNLDLRVHSLDVEDYIELTDNKEFKNLISS
GVEKVTRSQKDLFTLKYKRVQRIVPHNDTLIELFDQDIVMHMSELQLVLTPRSVLTLM
NYAMLTFTDPNAPEMPADVLRHNKEDRDDAPQKINMKIKMEAVNVIFNDDSIKLATLV
LSAGEFTMVLLPERYNINLKLGGLELTDETNESFSRDSVFRKIIQMKGQELVELSYES
FDPATNTKDYDSFLKYSTGSMHVNFIESAVNRMVNFFAKFQKSKVSFDRARLAAYNQA
PSIDAVNNMKMDIVIKAPIIQFPKLVGTQENNYDTMRFYLGEFFIENKFSVIDESHKI
NHIKLGVREGQLSSNLNFDGSSQQLYLVENIGLLFNIDRDPLPQDDTPELKVTSNFES
FALDLTENQLTYLLEISNKVSSAFNITDENSGESGGKGEIKSPSPDPASLSSESERTA
TPQSLQGSNKSNIKNPEQKYLDFSFKAPKIALTLYNKTKGVTSLNDCGLTRIMFQDIG
CSLGLKNDGTVDGQAHVAAFRIEDVRNIKDNKHTELIPKSKNKEYQFVANISRKNLEV
GRLLNISMTMDSPKMILAMDYLVSLKEFFDAIMSKSHENNLYYPENTNQKPENKAIVE
SVQEGGDVTKIQYSVNIIETALILLADPCDMNSEAISFKIGQFLVTDQNIMTVAANNV
GIFLFKMNSSEEKLRLLDDFSSSLTIDKRNSTPQTLMTNIQLSVQPLLMRISLRDIRL
AMLIFKRVTTLLNKMTEKEDNGEEEESTDKIQFSHEFERKLAVLDPSILGERSRASQS
SDSESIEVPTAILKNETFNADLGGLRFILIGDVHEMPILDMNVNEITASAKDWSTDFE
ALASLETYVNIFNYSRSSWEPLLEMIPITFHLSKGHSEMDPAFSFDILTQRIAEITLS
ARSIAMLSHIPASLTEELPLASRVSQKPYQLVNDTELDFDVWIQDKTTEDNKNEVVLL
KANTSLPWEFEDWRSIREKLDIDKSKNILGVCVSGQNYKTIMNIDATTEGENLHVLSP
PRNNVHNRIVCEARCDENNVKIITFRSTLVIENTTSTEIELLVDSKDPNKPSLKYAIK
PHQSKSVPVEYAYDSDIRIRPASEDIYDWSQQTLSWKSLLSNQMSIFCSSKEDSNQRF
HFEIGAKYDEREPLAKIFPHMKIVVSASMTIENLLPADINFSIFDKREEKRTDFLKTG
ESMEVHHISLDSFLLMSVQPLQDEASASKPSIVNTPHKSPLNPEDSLSLTLSGGQNLL
LKLDYKNIDGTRSKVIRIYSPYIIMNSTDRELYIQSSLLNIAQSKILLENEKRYTIPK
MFSFDKEDDKSNRARIRFKESEWSSKLSFDAIGQSFDASVSIKNKEQESNLGINISEG
KGKYLLSKVIEIAPRYIISNTLDIPIEVCETGSMDVQQIESNITKPLYRMRNIVDKQL
VLKFLGGDSNWSQPFFIKNVGVTYLKVLKNSRHKLLKIEILLDKATIFIRIKDGGDRW
PFSIRNFSDHDFIFYQRDPRKVSDPYKDDQSNESSSRSFKPIFYRIPSKSIMPYAWDF
PTAKEKYLVLESGTRTREVRLAEIGELPPLRLDKRSKDKPAPIVGLHVVADDDMQALV
IVNYKANVGLYKLKTASATTTSSVSVNSSVTDGFAQKDEDEKVNTQIVVSFKGVGISL
INGRLQELLYINMRGIELRYNESKAYQTFSWKMKWMQIDNQLFSGNYSNILYPTEIPY
TEKEIENHPVISGSISKVNDSLQAVPYFKHVTLLIQEFSIQLDEDMLYAMMDFIKFPG
SPWIMDSRDYKYDEEIQLPDVSELKTAGDIYFEIFHIQPTVLHLSFIRSDEISPGLAE
ETEESFSSSLYYVHMFAMTLGNINEAPVKVNSLFMDNVRVPLPILMDHIERHYTTQFV
YQIHKILGSADCFGNPVGLFNTISSGVWDLFYEPYQGYMMNDRPQEIGIHLAKGGLSF
AKKTVFGLSDSMSKFTGSMAKGLSVTQDLEFQRVRRLQQRINKNNRNALANSAQSFAS
TLGSGLSGIALDPYKAMQKEGAAGFLKGLGKGIVGLPTKTAIGFLDLTSNLSQGVKST
TTVLDMQKGCRVRLPRYVDHDQIIKPYDLREAQGQYWLKTVNGGVFMNDEYLSHVILP
GKELAVIVSMQHIAEVQMATQELMWSTGYPSIQGITLERSGLQIKLKSQSEYFIPISD
PEERRSLYRNIAIAVREYNKYCEAIL"
gene complement(48236..49381)
/gene="Ubi4"
/locus_tag="R008_L10156"
mRNA complement(48236..49381)
/gene="Ubi4"
/locus_tag="R008_L10156"
/product="Ubi4p"
CDS complement(48236..49381)
/gene="Ubi4"
/locus_tag="R008_L10156"
/note="Similar to YLL039C 'Ubiquitin, becomes conjugated
to proteins, marking them for selective degradation via
the ubiquitin-26S proteasome system; essential for the
cellular stress response; encoded as a polyubiquitin
precursor comprised of 5 head-to-tail repeats'"
/codon_start=1
/product="Ubi4p"
/protein_id="EWG84248.1"
/translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLI
FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNV
KSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL
TGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKEST
LHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGK
QLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVESSDTIDNVKSKI
QDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGN"
gene complement(49949..50692)
/gene="Ent4"
/locus_tag="R008_L10161"
mRNA complement(49949..50692)
/gene="Ent4"
/locus_tag="R008_L10161"
/product="Ent4p"
CDS complement(49949..50692)
/gene="Ent4"
/locus_tag="R008_L10161"
/note="Similar to YLL038C 'Protein of unknown function,
contains an N-terminal epsin-like domain; proposed to be
involved in the trafficking of Arn1p in the absence of
ferrichrome'"
/codon_start=1
/product="Ent4p"
/protein_id="EWG84249.1"
/translation="MPLLDTFKSFIQSPTESKVKQATNEDETSGATGTLMNEISILTY
SPKTVREIIQVIRKRLLLGQNRRNSHRNCIQVMKTLTLVSYLMNNGSNEFIKWLKGNM
ILIEILEDFQVQDPRDERKAEDIQKLSRNVLGLLQDDGLLEKQRKDVIQFRSSISTPG
RKSTDNSHLKLEEMRNELTRQSLEKKAKPPTTSTSLDFQRQRTRNTHEYARFSLDPLA
EEDSEDTPGVAGGISKLSFRPKSSNNPFR"
gene complement(50918..52429)
/gene="Prp19"
/locus_tag="R008_L10171"
mRNA complement(50918..52429)
/gene="Prp19"
/locus_tag="R008_L10171"
/product="Prp19p"
CDS complement(50918..52429)
/gene="Prp19"
/locus_tag="R008_L10171"
/note="Similar to YLL036C 'Splicing factor associated with
the spliceosome; contains a U-box, a motif found in a
class of ubiquitin ligases, and a WD40 domain'"
/codon_start=1
/product="Prp19p"
/protein_id="EWG84250.1"
/translation="MFCAISGKVPRRPVLSPKSRTIFEKSLLEQYVKDTGNDPITNEP
LSIEEIVEIVPNAQQASLTESTNSATLKANYSIPNLLTSLQNEWDAIMLENFKLRSTL
DSLTKKLSTVMYERDAAKLVAAQLLMEKNEDSKDLPKSSQQAVAITREEFLQGLLQSS
RDFVARGKLKAPKWPILKNLELLQAQNYSRNIKTFPYKELNKSMYYDKWVCMCRCEDG
ALHFTQLKDSKTITTITTPNPRTGGEHPAIISRGPCNRLLLLYPGNQITILDSKTNKV
LREIEVDSANEIIYMYGHNETNTEYFIWADNRGTIGFQSYEDDSQYIVHSAKSDVEYS
SGALHKDSLLLALYSPDGILDVYNLSSPDQASSRFPVDEEAKIKEVKFADNGYWMVVE
CDQTVVCFDLRKDVGTLAYPTYTIPEFKTGTVTYDIDDSGKNMIAYSNESNSLTIYKF
DKKTKNWTKDEESALCLQSDTADFTDMDVVCGDGGIAAILKTNDSFNIVALTP"
gene 52753..54651
/gene="Grc3"
/locus_tag="R008_L10176"
mRNA 52753..54651
/gene="Grc3"
/locus_tag="R008_L10176"
/product="Grc3p"
CDS 52753..54651
/gene="Grc3"
/locus_tag="R008_L10176"
/note="Similar to YLL035W 'Polynucleotide kinase present
on rDNA that is required for efficient transcription
termination by RNA polymerase I; required for cell growth;
mRNA is cell-cycle regulated'"
/codon_start=1
/product="Grc3p"
/protein_id="EWG84251.1"
/translation="MVIDSKQDLPQYTKDSGSESDSDSSNNFIVGSPSIPSSKSATVV
LNSEEYEDDEGDDLNGLDAELIDNITYEGDEDETMFVGLKEKQKLHLSGVFRLQVVKG
GIVYNNVHYNASREILTFWHPLSQSIPTIDFSHFAGWQDTIFMPRNNRFKIRDEEFKS
FPCVLRVFNSNHTGLLEAGHLYRDVNYLWKPKEPYFPLNERTTYHLLHESDRIQSLSV
PGYWSTPLEKLYLSHKNAAYDTRIMVIGGKNSGKSTFLRLLLEKFTQDIRDSTTSQEE
LVYLDLDPGQPEYSLPDSISLNKILSSPISLGQHLCQGSNFQTLLQFYAGSSSPQDEP
TSYLNCADKLIDHLEEQAFFGTSLLNLPGWIKGFGMQILNHIIRKYKPTHLLFLETAN
SKRHLDELTIPQSFSTSLRDAYAPEVVRVPAHSLNHTLSSRFHASQLRTFKILALFHK
ITQFDYDFAPLLKSAPLQISYGKGKSGIKGIQFPMEFQNLNPQDIKSALEGTVIGIYT
YSGEDSLEVKSLNTFPILQSCTSSSKNFITLGLIHSIDTSQQIMNIYVPPCHTQILDK
QPEDAQWIIVRNKTETPFCDFLPSPRTITWDDNIQIPFATFERRKKLEHVWKVRKNVM
RRGQFMKR"
gene complement(54807..57320)
/gene="Rix7"
/locus_tag="R008_L10181"
mRNA complement(54807..57320)
/gene="Rix7"
/locus_tag="R008_L10181"
/product="Rix7p"
CDS complement(54807..57320)
/gene="Rix7"
/locus_tag="R008_L10181"
/note="Similar to YLL034C 'Putative ATPase of the AAA
family, required for export of pre-ribosomal large
subunits from the nucleus; distributed between the
nucleolus, nucleoplasm, and nuclear periphery depending on
growth conditions'"
/codon_start=1
/product="Rix7p"
/protein_id="EWG84252.1"
/translation="MVKVKSKKNSLTSSLDNKIVDLIYRLLEEKTLDRKRSLRQESQG
EEGENNEGEKDEDIFESMFFAKDLTAGEIFTFCLTKDLSLQRVKKVVLQKTIDRMLKD
VIESELEEFGSYPGYNNEEEEKPSLEEELAKKNMMIERDTNEMNKRITSTWSKSGSVS
ESITETDDPKTGEVKKSKKRSKEGTCKVKRQKIKEDRSPPNSSLKSLGGMDDVVAQLM
ELIGLPILHPEIFLSTGVEPPRGVLLHGPPGCGKTSIANALAGELQVPFISISAPSVV
SGMSGESEKKIRDLFDEARSLAPCLVFFDEIDAITPKRDGGAQREMERRIVAQLLTSM
DELTMEKTNGKPVIIIGATNRPDSLDAALRRAGRFDREICLNVPNEVSRLHILKKMSD
NLKIDGAIDFAKLAKLTPGFVGADLKALVTAAGTCAIKRIFQTYANIKSTPTTATDSS
EDNMEIDETANGDESSLKNTANMIDPLPLSVVQQFIRNYPEPLSGEQLSLLSIKYEDF
LKALPTIQPTAKREGFATVPDVTWANVGALQRVRLELNMAIVQPIKRPELYEKVGISA
PGGVLLWGPPGCGKTLLAKAVANESRANFISIKGPELLNKYVGESERSIRQVFTRARA
SVPCVIFFDELDALVPRRDTSLSESSSRVVNTLLTELDGLNDRRGIFVIGATNRPDMI
DPAMLRPGRLDKTLFIELPNTEEKLDIIKTLTKSHGTPLSSDVDFEEIIRNEKCNNFS
GADLAALVRESSVLALKRKFFQSEEIQSVLDNDLDKEFEDLSVGVSGEEIIVTMSDFR
SALRKIKPSVSDKDRLKYDRLNKKMGLTEEMKDAEEMKQ"
gene 57583..58275
/gene="Irc19"
/locus_tag="R008_L10186"
mRNA 57583..58275
/gene="Irc19"
/locus_tag="R008_L10186"
/product="Irc19p"
CDS 57583..58275
/gene="Irc19"
/locus_tag="R008_L10186"
/note="Similar to YLL033W 'Putative protein of unknown
function; YLL033W is not an essential gene but mutant is
defective in spore formation; null mutant displays
increased levels of spontaneous Rad52p foci'"
/codon_start=1
/product="Irc19p"
/protein_id="EWG84253.1"
/translation="MRKPSITITTAKAIITPDYTLIKSHSKYQLPSRFQKLDADSPER
STVVKLFYRRFMRLKPFISNVKMVKDTYRDYVRYKFMKENYELKRYLVFNPDGLRSKI
KLELLSNTKCCERILPVTEMQRTLEFVLKSCSYLPETKAQKWDIARDNTYCRQILKNL
LTMQYEKYRSILHRGIGHDELDVKFSHLKTTSSPLTKLNKTEKKKIPLFKVFSDFDTT
LIYLNETLGTRL"
gene complement(58444..60921)
/locus_tag="R008_L10191"
mRNA complement(58444..60921)
/locus_tag="R008_L10191"
/product="hypothetical protein"
CDS complement(58444..60921)
/locus_tag="R008_L10191"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84254.1"
/translation="MDNFKIYSTVITTAFLQVPHLYTTNRLWKPIEAPFLVEFLQKRI
SSKEIKNTKAICHIDPSWVNLNASFIRDDMISIKATTDDMDLDAICRISLPLPMNTND
LTAELEKMKRILLDLSEKFNLELIITKEPAYFTPEQTGESKELCIYVHALGFRSNLME
CEPQLLAFVDLIKKNGMTLPPQHYIIEPMELNSYSVLPLYMGVDMENFKHISRAFKTS
IYAPSLITLSRDFKANPQIFFSGAVHSLSLLARKTLRESISVNSKSFFYRRLTNITPG
KLLFIQKYYQQKVNQLILKYQSLIRVTNEYIEFQSISTNLLEMVIKNFTIQVLHEIVE
VQISLNENCAMSPELIIDSFFGHTGNQIVVITPKEDSFNQLIVVGNQSSTDEASDTSI
LHYLSDFIMGSNQVINPNLRQIKAIFEIHPDFEDFISGKKNGKLTRIMELSACLIQLE
MEEEDDNLYLNLVSDSFPDFKESFKNVINEFPAEESFFIPEVCHRPIIGTGGSLIQAT
MRKHNVFIQFSNSFNLPQNKISMIRYDNVIIRCPRKNKANICLAKKDLKQIVQEYDSL
QSKTLIRFSSGQYRHILHVNGQKNIIGQIEKNENVYIMIPLKEPLDGTSQLSIQGNDE
NTSRAANELVNSAFGYEYEFKIDQEIDPNKEYEFYNLIVVPFLQIMNIIVTFEKDLIT
FTFEKDTNENTLTKAIELLSDYLETQKTKIIFKKIIKKFVLGSASSKSNTSNSNTNGN
FRSMNNAKSRTTIDNTSQSGASPQRHKMPVITTVGGAQAIKGYIPNTYYNGYGYGYGY
TYEYDYNYANSNKAQTNNRHKYQNGRK"
gene complement(61326..64378)
/gene="Gpi13"
/locus_tag="R008_L10196"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(64528..64869)
/locus_tag="R008_L10201"
mRNA complement(64528..64869)
/locus_tag="R008_L10201"
/product="hypothetical protein"
CDS complement(64528..64869)
/locus_tag="R008_L10201"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84255.1"
/translation="MYFAFKGLCGRRFLPIASFLTILNRILFQYWLFYNSLKEEKTFQ
KIFFSMNLKCRKKKEPNHIIYSQPPFLLGFMVQLQSCVKLLPLYLLFLLQGNGAHYAS
AMTSKVLSFFL"
gene 65635..67884
/gene="Rup2"
/locus_tag="R008_L10206"
mRNA 65635..67884
/gene="Rup2"
/locus_tag="R008_L10206"
/product="Rup2p"
CDS 65635..67884
/gene="Rup2"
/locus_tag="R008_L10206"
/note="Similar to YLL029W 'Protein involved in negative
regulation of transcription of iron regulon; forms an iron
independent complex with Fra2p, Grx3p, and Grx4p;
cytosolic; mutant fails to repress transcription of iron
regulon and is defective in spore formation'"
/codon_start=1
/product="Rup2p"
/protein_id="EWG84256.1"
/translation="MTSKPSTSDGRAHSISHVPGTHMRGTSASHSPRPFRPCADCTCS
PGLLSRQGRRASLFLRQLENSRRSSSMLLNELKGAGGGSSAGNGSVYSCDSLCAVNRE
VNTTDRLLKLRQEMKKHDLCCYIVPSCDEHQSEYVSLRDQRRAFISGFSGSAGVACIT
RDLLNFNDDHPDGKSILSTDGRYFNQARQELDYNWTLLRQNEDPITWQEWCVREALEM
AKGLGNKEGMVLKIGIDPKLITFNDYVSFRKMIDTKYDAKGKVELVPVEENLVDSIWP
DFETLPERPCNDLLLLKYEFHGEEFKDKKEKLLKKLNDKASSATTGRNTFIVVALDEI
CWLLNLRGSDIDYNPVFFSYVAINEDETILFTNNPFNDDISEYFKINGIEVRPYEQIW
EHLTKITSQASSAEHEFLIPDSASWQMVRCLNTSTNANGAVAKKMTALNFAMIHSPID
VLKSIKNDIEIKNAHKAQVKDAVCLVQYFAWLEQQLVGREALIDEHRAAEKLTEIRKT
QRNFMGNSFETISSTGSNAAIIHYSPPVENSSMIDPTKIYLCDSGSQFLEGTTDITRT
IHLTKPTKEEMDNYTLVLKGGLALERLIFPENTPGFNIDAIARQFLWSRGLDYKHGTG
HGIGSFLNVHEGPMGVGFRPHLMNFPLRAGNIISNEPGYYKDGEYGIRIESDMLIKKA
TEKGNFLKFENMTVVPYCRKLINTKLLNEEEKTQINEYHARVWRTIVHFLQPQSISYK
WLKRETSPL"
gene 68976..70746
/gene="Tpo1"
/locus_tag="R008_L10211"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 71588..72340
/gene="Isa1"
/locus_tag="R008_L10216"
mRNA 71588..72340
/gene="Isa1"
/locus_tag="R008_L10216"
/product="Isa1p"
CDS 71588..72340
/gene="Isa1"
/locus_tag="R008_L10216"
/note="Similar to YLL027W 'Mitochondrial matrix protein
involved in biogenesis of the iron-sulfur (Fe/S) cluster
of Fe/S proteins, isa1 deletion causes loss of
mitochondrial DNA and respiratory deficiency; depletion
reduces growth on nonfermentable carbon sources'"
/codon_start=1
/product="Isa1p"
/protein_id="EWG84257.1"
/translation="MINTGRSRNSVLLAHRFLSTGGFWRGGTNGTMSRTINNVNPFKL
KFIPKTVPAAADSVSPDSQRPGKKPFKFIVSNQSKSSKASKSPKWSSYAFPSRETIKS
HEEAIKKQNKAIHEQIAAAVSKNDCSCTEPPKKRKRKLRPRKALITLSPKAIKHLRAL
LAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDGVKIVIDSKALFSVIGS
EMDWVDDKLASKFVFKNPNSKGTCGCGESFMV"
gene 72808..75534
/gene="Hsp104"
/locus_tag="R008_L10221"
mRNA 72808..75534
/gene="Hsp104"
/locus_tag="R008_L10221"
/product="Hsp104p"
CDS 72808..75534
/gene="Hsp104"
/locus_tag="R008_L10221"
/note="Similar to YLL026W 'Heat shock protein that
cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold
and reactivate previously denatured, aggregated proteins;
responsive to stresses including: heat, ethanol, and
sodium arsenite; involved in [PSI+] propagation'"
/codon_start=1
/product="Hsp104p"
/protein_id="EWG84258.1"
/translation="MNDQTQFTERALTILTLAQKLASDHQHPQLQPIHILAAFIETPE
DGSVPYLQNLIEKGRYDYDLFKKVVNRNLVRIPQQQPAPAEITPSYALGKVLQDAAKI
QKQQKDSFIAQDHILFALFNDSSIQQIFKEAQVDIEAIKQQALELRGNTRIDSRGADT
NTPLEYLSKYAIDMTEQARQGKLDPVIGREEEIRSTIRVLARRIKSNPCLIGEPGIGK
TAIIEGVAQRIIDDDVPTILQGAKLFSLDLAALTAGAKYKGDFEERFKGVLKEIEESK
TLIVLFIDEIHMLMGNGKDDAANILKPALSRGQLKVIGATTNNEYRSIVEKDGAFERR
FQKIEVAEPSVRQTVAILRGLQPKYEIHHGVRILDSALVTAAQLAKRYLPYRRLPDSA
LDLVDISCAGVAVARDSKPEELDSKERQLQLIQVEIKALERDEDADSTTKDRLKLARQ
KEASLQEELEPLRQRYNEEKHGHEELTQAKKKLDELENKALDAERRYDTATAADLRYF
AIPDIKKQIEKLEDQVAEEERRAGANSMIQNVVDSDTISETAARLTGIPVKKLSESEN
EKLIHMERDLSSEVVGQMDAIKAVSNAVRLSRSGLANPRQPASFLFLGLSGSGKTELA
KKVAGFLFNDEDMMIRVDCSELSEKYAVSKLLGTTAGYVGYDEGGFLTNQLQYKPYSV
LLFDEVEKAHPDVLTVMLQMLDDGRITSGQGKTIDCSNCIVIMTSNLGAEFINSQQGS
KIQESTKNLVMGAVRQHFRPEFLNRISSIVIFNKLSRKAIHKIVDIRLKEIEERFEQN
DKHYKLNLTQEAKDFLAKYGYSDDMGARPLNRLIQNEILNKLALRILKNEIKDKETVN
VVLKKGKSRDENVPEEAEECLEVLPNHEATIGADTLGDDDNEDSMEIDDDLD"
gene 76731..76833
/locus_tag="R008_L10226"
tRNA 76731..76833
/locus_tag="R008_L10226"
/product="tRNA-Pro"
/note="TP(UGG)L"
gene 78930..79304
/gene="Pau17"
/locus_tag="R008_L10241"
mRNA 78930..79304
/gene="Pau17"
/locus_tag="R008_L10241"
/product="Pau17p"
CDS 78930..79304
/gene="Pau17"
/locus_tag="R008_L10241"
/note="Similar to YLL025W 'Protein of unknown function,
member of the seripauperin multigene family encoded mainly
in subtelomeric regions; YLL025W is not an essential
gene'"
/codon_start=1
/product="Pau17p"
/protein_id="EWG84259.1"
/translation="MVKLTSIAAGVAAIAAGVAAAPATTTLSPSDERVNLVELGVYVS
DIRAHLAEYYMFQAAHPTETYPVEIAEAVFNYGDFTTMLTGIPADQVTRVITGVPWYS
TRLRPAISSALSADGIYTAVPN"
gene complement(79994..81684)
/gene="Ssa2"
/locus_tag="R008_L10246"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(82196..83035)
/locus_tag="R008_L10256"
mRNA complement(82196..83035)
/locus_tag="R008_L10256"
/product="hypothetical protein"
CDS complement(82196..83035)
/locus_tag="R008_L10256"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84260.1"
/translation="MSSRPANNQGPPNLPARDKSLVQRFMAVAKSLQFAWFTGHSVVL
ISSILYLLKMSEFYYRLAYLGVIESFGIIIYQQFFTRNEPLQTQDAAATKASIKSRVA
GLLKSEDVLYLVLANFWLFTPRFSFSLIPFFAFAVFHVLIYVEKVLLPKVFHLSSKDS
SKILSFIDKFVVQYNDLCMHWVGTAELLIFILVLFRAILCFQRSWIILVVYAIFIKLR
YENSKYMKAAFAQWRVRMDGIISHPSIPPFVKRAYNAIKMSLVRLSEYRLSGAPQVTK
KQN"
gene complement(83243..84400)
/gene="Hif1"
/locus_tag="R008_L10261"
mRNA complement(83243..84400)
/gene="Hif1"
/locus_tag="R008_L10261"
/product="Hif1p"
CDS complement(83243..84400)
/gene="Hif1"
/locus_tag="R008_L10261"
/note="Similar to YLL022C 'Non-essential component of the
HAT-B histone acetyltransferase complex
(Hat1p-Hat2p-Hif1p), localized to the nucleus; has a role
in telomeric silencing'"
/codon_start=1
/product="Hif1p"
/protein_id="EWG84261.1"
/translation="MKLRAEDVLANGTSRHKLQIDMERQIQIAKDLLAQKKFLEAAKR
CQQTLDSLPKDGLLPDPELFTIFAQAVYNMEVQNSGNLFGDALLAGDDGSGSESESEP
ESDVSNGEEGNENGQTEIPNSRMFQFDQEEEDLTGDVDSGDSEDSGEGSEEEEENVEK
EEERLALHELANFSPANEHDDEIEDVSQLRKSGFHIYFENDLYENALDLLAQALMLLG
RPTADGQSLTENSRLRIGDVYILMGDIEREAEMFSRAIHHYLKALGYYKTLKPAEQVT
EKVIQAEFLVCDALRWVDQVPAKDKLKRFKHAKALLEKHMTTRPKDSELQQARLAQIQ
DDIDEVQENQQHGSKRPLSQPTTSIGFPALEKPLGDFNDLSQLVKKKPRRH"
gene 85155..89727
/gene="Spa2"
/locus_tag="R008_L10266"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(90067..92280)
/gene="Kns1"
/locus_tag="R008_L10276"
mRNA complement(90067..92280)
/gene="Kns1"
/locus_tag="R008_L10276"
/product="Kns1p"
CDS complement(90067..92280)
/gene="Kns1"
/locus_tag="R008_L10276"
/note="Similar to YLL019C 'Nonessential putative protein
kinase of unknown cellular role; member of the LAMMER
family of protein kinases, which are serine/threonine
kinases also capable of phosphorylating tyrosine
residues'"
/codon_start=1
/product="Kns1p"
/protein_id="EWG84262.1"
/translation="MSQNIQIGTRKRSRANMNNSTTTGPANNTSSNKTFLDNFEETRT
NKLLDEMFARQNSFLTDNLRNSLDLNQADNPLRPRQHQHQLFLDNENAIELDEEPRII
NTTINNSNNHNSSRVDEDADDDIIFIKEQPIQFSSPLILPSSSSINNNNNIVTSNNPG
CGTAVTSNSTYITTPKKFKKQRTISLPQLPLSKLSYQSNYFNVPDQTNAIVPRVTQTE
NELLHLTGSCAKTLEGNKAVNLTIAHSTSPFYNPPAQIASLSQSNLKKQIGSSLRKFT
SNGSSESASSNKSNFKTDKDGHYVYQENDIFGSGGRFVVKDLLGQGTFGKVLKCIDNK
YEPNYVAVKVIRAVDRYREAAKTELRILQTILNNDPQGQFQCLLLRECFDYKNHICLV
TDLYGRSIYDFMCSNGIARFPGSHIQAIARQLIRSVCFLHDLGIIHTDLKPENILICD
ETHIAQKLPLKTVQSLSKRRREASKGKRKILKNPEIKIIDFGSAIFHYEYHPPVISTR
HYRAPEIVLGLGWSFPCDIWSIACVLVELVIGESLYPIHENLEHMAMMQRINGTPFPT
DIIDKMFYKSKHKLGNSPSDLNSTVIKHFDRKSLSLQWPEKNKRGDTITTEKSMKRVL
QSCDRLDIYISKVLKQDYGDSLSINWNLPPEKNWSLINSKLAWKKQTHSSSSSTTDEL
DKETFLFWYWFIDLLRKMFEFDPTKRITAKDALDHEWFNLGILDDGIATYNNTQG"
gene complement(93056..93352)
/gene="Cox19"
/locus_tag="R008_L10286"
mRNA complement(93056..93352)
/gene="Cox19"
/locus_tag="R008_L10286"
/product="Cox19p"
CDS complement(93056..93352)
/gene="Cox19"
/locus_tag="R008_L10286"
/note="Similar to YLL018C-A no_gene_description"
/codon_start=1
/product="Cox19p"
/protein_id="EWG84263.1"
/translation="MSGNPGSSLSALRPTPPERGSFPLDHDGECTKYMQEYLKCMQLV
QNENAMNCRLLAKDYLRCRMDHQLMDYDEWSHLGLPEDAPGNNGKTIKDATDNK"
gene complement(94283..95956)
/gene="Dps1"
/locus_tag="R008_L10291"
mRNA complement(94283..95956)
/gene="Dps1"
/locus_tag="R008_L10291"
/product="Dps1p"
CDS complement(94283..95956)
/gene="Dps1"
/locus_tag="R008_L10291"
/note="Similar to YLL018C 'Aspartyl-tRNA synthetase,
primarily cytoplasmic; homodimeric enzyme that catalyzes
the specific aspartylation of tRNA(Asp); class II
aminoacyl tRNA synthetase; binding to its own mRNA may
confer autoregulation'"
/codon_start=1
/product="Dps1p"
/protein_id="EWG84264.1"
/translation="MSQDENIVKAVEESAEPAQVILGEDGKPLSKKALKKLQKEQEKQ
RKKEERALQLEAEREAREKKAAAEDTAKDNYGKLPLIQSRDSDRTGQKRVKFVDLDEA
KDSDKEVLFRARVHNTRQQGATLAFLTLRQQASLIQGLVKANKEGTISKNMVKWAGSL
NLESIVLVRGIVKKVDEPIKSATVQNLEIHITKIYTISETPEALPILLEDASRSEAEA
EAAGLPVVNLDTRLDYRVIDLRTVTNQAIFRIQAGVCELFREYLATKKFTEVHTPKLL
GAPSEGGSSVFEVTYFKGKAYLAQSPQFNKQQLIVADFERVYEIGPVFRAENSNTHRH
MTEFTGLDMEMAFEEHYHEVLDTLSELFVFIFSELPKRFAHEIELVRKQYPVEEFKLP
KDGKMVRLTYKEGIEMLRAAGKEIGDFEDLSTENEKFLGKLVRDKYDTDFYILDKFPL
EIRPFYTMPDPANPKYSNSYDFFMRGEEILSGAQRIHDHALLQERMKAHGLSPEDPGL
KDYCDGFSYGCPPHAGGGIGLERVVMFYLDLKNIRRASLFPRDPKRLRP"
gene 96609..100367
/gene="Sdc25"
/locus_tag="R008_L10306"
mRNA 96609..100367
/gene="Sdc25"
/locus_tag="R008_L10306"
/product="Sdc25p"
CDS 96609..100367
/gene="Sdc25"
/locus_tag="R008_L10306"
/note="Similar to YLL016W Putative protein of unknown
function, possibly merged to YLL017W"
/codon_start=1
/product="Sdc25p"
/protein_id="EWG84265.1"
/translation="MSRTASYAGMTTPVKDKEGHGIPCLQPIDVVECTYQYFTKSQNK
LSLRVGDLIYVLTKGSNGWWDGVLIRHSANNNNNSLILDRGWFPPSFTRSILNELHGV
PEIGNELEIFQAGLNLKLELSSNPVILSLEDFLDCCRDIEFKEQLAWSPIPVHERKGC
CELLYYNQDLDVYCRTLPYLPQNQVETVNDYSSFPAISKIAGKKMPITSSPDLFYLND
CDVVYWYDLTRLVCHYVNLTERDLLANEREKFLTSLDLLTAQITCVYMLFRNLRLVED
SFKKTLKKLIYTLSRFSINANIWFHSTPFEEREAIASQKDPERRSPLLQSILGTFQKF
HFLLRLLHFLSNPNELTILPQLTPRFFKDSFNTISWNNPFLRKHLNQHMSHDLPRQMI
KAVAGASGIVAENNDEIPASKQGTSCSSETSHHSPSAPFQRRRRGTIFSNVPGSSDES
DTIWSKRKKPYPLNEETLSLVRARKEQLDAKLKQMIKSANEYLSNTANFSKMLNFEMN
FKTYEEVSGTIPIIDILENLDLTIYLNLRELGDENRVFDEDVAIDDEDKEFLKHSLSS
LSYILSDYFNMKQYFHDVVVKFIIVAQHLTLEDPFVFSPMQNDLPTGYYEPMKPSSLN
LDNAKDKKNGSQNTDIQEEEDEYEPDPDSLILFHNLINQDSDFNDLKFFNLAHVFKKS
CDDYFDVLKLSIEFVNRLILERENLLNYAARMMKNNITELLLRGEEGYGSYDGGETAE
KSDTNAVYADSDTKDNDEWRDSQVKLPRYLQREYDSELIWGSNNRIKGGSKHALISYL
TDNEKKDLFFNITFLITFRSIFTTTEFLSYLISQYNLDPPEDLCFEEYNEWVTKKLIP
VKCRVVEIMTTFFKQYWFPGYDEPDLATLNLDYFAQVAIKENITGSVELLKEVNQKFK
HGNMQEATAPMKTLDQQICQEHYWGTLYSTTESILAVDPVLFATQLTILEHEIYCEIT
IFDCLQKIWKNKYTKSYGASPGLNEFISFANKLTNFISYSIVKEADKSKRAKLLSHFI
FIAEYCRKFNNFSSMTAIISALYSSSIYRLEKTWQAVIPQTRDLLQSLDKLMDPKKNF
INYRSELKSLHSAPCVPFFGVYLSDLTFTDSGNPDYLVLEHGLKGVHDEKKYINFNKR
SRLVDILQEIIYFKKTHYDFTKDRTVIECISNSLENIPHIEKQYQLSLIIEPKPRKKV
VPNSNSNNKSQEKSRDDQTDEGKTSTKKDRFSKFQLHKTKKKAPKVSK"
gene 100806..105485
/gene="Bpt1"
/locus_tag="R008_L10311"
mRNA 100806..105485
/gene="Bpt1"
/locus_tag="R008_L10311"
/product="Bpt1p"
CDS 100806..105485
/gene="Bpt1"
/locus_tag="R008_L10311"
/note="Similar to YLL015W ABC type transmembrane
transporter of MRP/CFTR family, found in vacuolar
membrane, involved in the transport of unconjugated
bilirubin and in heavy metal detoxification via
glutathione conjugates, along with Ycf1p"
/codon_start=1
/product="Bpt1p"
/protein_id="EWG84266.1"
/translation="MSSLEVVDGCPYGYRPYPDSGTNALNPCFISVISAWQAVFFLLI
GSYQLWKLYKNNKVPPRFKNFPTLPSKINSRHLTHLTNVCFQSTLIICELALVSQSSD
RVYPFILKKALYLNLLFNLGISLPTQYLAYFKSTFSMGNQLFYYMFQILLQLFLILQR
YYHGSSNERLTVISGQTAMILEVLLLFNSVAIFIYDLCIFEPINELSEYYKKNGWYPP
VHVLSYITFIWMNKLIVETYRNKKIKDPNQLPLPPVDLNIKSISKEFKANWELEKWLN
RNSLWRAIWKSFGRTISVAMLYETTSDLLSVVQPQFLRIFIDGFNPETSSKYPPLNGV
FIALTLFVISVVSVFLTNQFYIGIFEAGLGIRGSLASLVYQKSLRLTLAERNEKSTGD
ILNLMSVDVLRIQRFFENAQTIIGAPIQIIVVLTSLYWLLGKAVIGGLVTMAIMMPIN
AFLSRKVKKLSKTQMKYKDMRIKTITELLNAIKSIKLYAWEEPMMARLNHVRNDMELK
NFRKIGIVSNLIYFAWNCVPLMVTCSTFGLFSLFSDSPLSPAIVFPSLSLFNILNSAI
YSVPSMINTIIETSVSMERLKSFLLSDEIDDSFIERIDPSADERALPAIEMNNITFLW
KSKEVLASSQSRDNLRTDEESIIGSSQIALKNIDHFEAKRGDLVCVVGRVGAGKSTFL
KAILGQLPCMSGSRDSIPPKLIIRSSSVAYCSQESWIMNASVRENILFGHKFDQNYYD
LTIKACQLLPDLKILPDGDETLVGEKGISLSGGQKARLSLARAVYSRADIYLLDDILS
AVDAEVSKNIIEYVLIGKTALLKNKTIILTTNTVSILKHSQMIYALENGEIVEQGNYE
DVMNRKNNTSKLKKLLEEFDSPIDNGNESDVQTEHRSESEVDEPLQLKVTESETEDEV
VTESELELIKANSRRASLATLRPRPFVGAQLDSVKKTAQEAEKTEVGRVKTKVYLAYI
KACGVLGVVLFFLFMILTRVFDLAENFWLKYWSESNEKNGSNERVWMFVGVYSLIGVA
SAAFNNLRSIMMLLYCSIRGSKKLHESMAKSVIRSPMTFFETTPVGRIINRFSSDMDA
VDSNLQYIFSFFFKSILTYLVTVILVGYNMPWFLVFNMFLVVIYIYYQTFYIVLSREL
KRLISISYSPIMSLMSESLNGYSIIDAYDHFERFIYLNYEKIQYNVDFVFNFRSTNRW
LSVRLQTIGATIVLATAILALATMNTKRQLSSGMVGLLMSYSLEVTGSLTWIVRTTVM
IETNIVSVERIVEYCELPPEAQSINPEKRPDENWPSKGGIEFKNYSTKYRENLDPVLN
NINVKIEPCEKVGIVGRTGAGKSTLSLALFRILEPTEGKIIIDGIDISDIGLFDLRSH
LAIIPQDAQAFEGTVKTNLDPFNRYSEDELKRAVEQAHLKPHLEKMLHSKPRGDDSNE
EDGNVNDILDVKINENGSNLSVGQRQLLCLARALLNRSKILVLDEATASVDMETDKII
QDTIRREFKDRTILTIAHRIDTVLDSDKIIVLDQGSVREFDSPSKLLSDKTSIFYSLC
EKGGYLK"
gene 105696..106022
/locus_tag="R008_L10316"
mRNA 105696..106022
/locus_tag="R008_L10316"
/product="hypothetical protein"
CDS 105696..106022
/locus_tag="R008_L10316"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84267.1"
/translation="MSSNEEVFTQINATANVVDNKKRLLFVQDSSALVLGLVAGFLQI
ESVHGFIWFLILYNLINVIYIVWICQLQPGKFYQSPLQDIFFESFFREITGFVMAWTF
GYALIG"
gene complement(106443..109082)
/gene="Puf3"
/locus_tag="R008_L10321"
mRNA complement(106443..109082)
/gene="Puf3"
/locus_tag="R008_L10321"
/product="Puf3p"
CDS complement(106443..109082)
/gene="Puf3"
/locus_tag="R008_L10321"
/note="Similar to YLL013C 'Protein of the mitochondrial
outer surface, links the Arp2/3 complex with the mitochore
during anterograde mitochondrial movement; also binds to
and promotes degradation of mRNAs for select
nuclear-encoded mitochondrial proteins'"
/codon_start=1
/product="Puf3p"
/protein_id="EWG84268.1"
/translation="MEMNMDMDMDMELASIVSSLSALSNSNNNGGQAAAAGIVNGGAA
GSQQIGGFRRSSFTTANEVDSEILLLHGSSESSPIFKKTALSVGTAPPFSTNSKKFFG
NGGNYYQYRSTDTASLSSASYNNYHTHHTAANLGKNNKVNHLLGQYSASIAGPVYYNG
NDNNNSGGEGFFEKFGKSLIDGTRELESQDRPDAVNTQSQFISKSVSNASLDTQNTFE
QNVESDKNFNKLNRNTTNSGSLYHSSSNSGSSASLESENAHYPKRNIWNVANTPVFRP
SNNPAAVGATNVALPNQQDGPANNNFPPYMNGFPPNQFHQGPHYQNFPNYLIGSPSNF
ISQMISVQIPANEDTEDSNGKKKKKANRPSSVSSPSSPPNNSPFPFAYPNPMMFMPPP
PLSAPQQQQQQQQQQQQEDQQQQQQQENPYIYYPTPNPIPVKMPKDEKTFKKRNNKNH
PANNSNNANKQANPYLENSIPTKNTSKKNASSKSNESTANNHKSHSHSHPHSQSLQQQ
QQTYHRSPLLEQLRNSSSDKNSNSNMSLKDIFGHSLEFCKDQHGSRFIQRELATSPAS
EKEVIFNEIRDDAIELSNDVFGNYVIQKFFEFGSKIQKNTLVDQFKGNMKQLSLQMYA
CRVIQKALEYIDSNQRIELVLELSDSVLQMIKDQNGNHVIQKAIETIPIEKLPFILSS
LTGHIYHLSTHSYGCRVIQRLLEFGSSEDQESILNELKDFIPYLIQDQYGNYVIQYVL
QQDQFTNKEMVDIKQEIIETVANNVVEYSKHKFASNVVEKSILYGSKDQKDSIISKIL
PRDKNHALNLEDDSPMILMIKDQFANYVIQKLVNVSEGEGKKLIVIAIRAYLDKLNKS
NSLGNRHLASVEKLAALVENAEV"
gene 109903..111624
/gene="Yeh1"
/locus_tag="R008_L10326"
mRNA 109903..111624
/gene="Yeh1"
/locus_tag="R008_L10326"
/product="Yeh1p"
CDS 109903..111624
/gene="Yeh1"
/locus_tag="R008_L10326"
/note="Similar to YLL012W 'Steryl ester hydrolase, one of
three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for
steryl ester hydrolase activity and involved in sterol
homeostasis; localized to lipid particle membranes'"
/codon_start=1
/product="Yeh1p"
/protein_id="EWG84269.1"
/translation="MGVSAVLKRARNLLATFIVCCFMAVVLVLALAHHFINEHRDTRS
SSTQIEVDDESKRNVHHDHVLTRTNAYATPYLDLEHDKKNGIVYDHTRTVVRKKNHEV
GSSPLHKNLFHKFLTKLIFRFIEKEKVTEGVTQGKFNNSSNEIANHEPVFEKIPVQCD
DPLQNLILSEDLTLVADLNYYFNQYNIQIEEFRLETEDGFVIDLWHLIPKYRTTDSDK
KKRPPVLMLHGLLQSSGSFASNGRKSLAYFLYQSGYDIWLGNNRCGFRPEWNEAKVPT
LASRWDWDLREMVKYDLTLLIDTVLAKTQFEKLTLISHSQGTTQGFMGLVNEDKFFPP
GSGSKESFFTSKIANYIALAPAVYPGPLLNEKLFVKLMTKEIDNPWFFGETSFFEIMM
IVRNLCVGESLFSFVCYTIFNYLFDWNDTLWDTALRDRHFLFSPVHVSVKLMQWWLSP
DPNKVSFKFGSHKMFPDNVKWFSDASKAPNIYLFVPKQDRLVDGERLINHFVNVESNV
NYKIWYIDEYAHIDVLWAHDVIERIGKPILQNLNNYYSKKPSSAFESDCSDTEVETEL
EMVAEKA"
gene 111893..113362
/gene="Sof1"
/locus_tag="R008_L10331"
mRNA 111893..113362
/gene="Sof1"
/locus_tag="R008_L10331"
/product="Sof1p"
CDS 111893..113362
/gene="Sof1"
/locus_tag="R008_L10331"
/note="Similar to YLL011W 'Essential protein required for
biogenesis of 40S (small) ribosomal subunit; has
similarity to the beta subunit of trimeric G-proteins and
the splicing factor Prp4p'"
/codon_start=1
/product="Sof1p"
/protein_id="EWG84270.1"
/translation="MKIKTIKRSADDYVPVKSTQESQMPRNLNPELHPFERAREYTKA
LNATKLERMFAKPFVGQLGYGHRDGVYAIAKNYGSLNKLATGSADGVIKYWNMSTREE
FVSFKAHYGLVTGLCVTQPRFHDKKPDLKSQNFMLSCSDDKTVKLWSINVDDYSNKNS
SDNDSVTNEEGLIRTFDGESAFQGIDSHRENSTFATGGAKIHLWDVNRLKPVSDLSWG
ADNITSLKFNQNETDILASTGSDNSIVLYDLRTNSPTQKIVQTMRTNAICWNPMEAFN
FVTANEDHNAYYYDMRNLSRSLNVFKDHVSAVMDVDFSPTGDEIVTGSYDKSIRIYKT
NHGHSREIYHTKRMQHVFQVKYSMDSKYIISGSDDGNVRLWRSKAWERSNVKTTREKN
KLEYDEKLKERFRHMPEIKRISRHRHVPQVIKKAQEIKNIELSSIKRREANERRTRKD
MPYISERKKQIVGTVHKYEDSGRDRKRRKEDDKRDTQEK"
gene complement(113697..114977)
/gene="Psr1"
/locus_tag="R008_L10336"
mRNA complement(113697..114977)
/gene="Psr1"
/locus_tag="R008_L10336"
/product="Psr1p"
CDS complement(113697..114977)
/gene="Psr1"
/locus_tag="R008_L10336"
/note="Similar to YLL010C 'Plasma membrane associated
protein phosphatase involved in the general stress
response; required along with binding partner Whi2p for
full activation of STRE-mediated gene expression, possibly
through dephosphorylation of Msn2p'"
/codon_start=1
/product="Psr1p"
/protein_id="EWG84271.1"
/translation="MGFISSILCCSSETTQSNSNSAYRQQQSSSLNKNRSVKHSNTKS
RTRGVHQTNSPPSKTNSAATFSSTERSTGKSGISTNDNEKKKPSSPTAAVTATTTNNM
TKVEKRISKDDLYEEKYEVDEDEEIDDEDNRRSRGIVQEKGDTVKDTSRQKKQQQQQQ
QSQPQPQPQSQSQSQSQSQSQQRGPTVQVSSDHLIQDMNLSKVSSSSQASETSNDADD
EDDEDEEYIDLTLLQQGQYHAPGYNTLLPPQDESTKGKKCLILDLDETLVHSSFKYLR
SADFVLPVEIDDQVHNVYVIKRPGVEEFLERVGKLFEVVVFTASVSRYGDPLLDILDT
DKVIHHRLFREACYNYEGNYIKNLSQIGRPLSDIIILDNSPASYIFHPQHAIPISSWF
SDTHDNELLDIIPLLEDLSVKTSLDVGKILDVTI"
gene complement(115578..115787)
/gene="Cox17"
/locus_tag="R008_L10341"
mRNA complement(115578..115787)
/gene="Cox17"
/locus_tag="R008_L10341"
/product="Cox17p"
CDS complement(115578..115787)
/gene="Cox17"
/locus_tag="R008_L10341"
/note="Similar to YLL009C 'Copper metallochaperone that
transfers copper to Sco1p and Cox11p for eventual delivery
to cytochrome c oxidase; contains twin
cysteine-x9-cysteine motifs'"
/codon_start=1
/product="Cox17p"
/protein_id="EWG84272.1"
/translation="MTETDKKQEQENHAECEDKPKPCCVCKPEKEERDTCILFNGQDS
EKCKEFIEKYKECMKGYGFEVPSAN"
gene 116102..118366
/gene="Drs1"
/locus_tag="R008_L10346"
mRNA 116102..118366
/gene="Drs1"
/locus_tag="R008_L10346"
/product="Drs1p"
CDS 116102..118366
/gene="Drs1"
/locus_tag="R008_L10346"
/note="Similar to YLL008W 'Nucleolar DEAD-box protein
required for ribosome assembly and function, including
synthesis of 60S ribosomal subunits; constituent of 66S
pre-ribosomal particles'"
/codon_start=1
/product="Drs1p"
/protein_id="EWG84273.1"
/translation="MVVGTKKYSNLDFVPTISDSEDDVPILDSSDDEKVEAKKTTKKR
KGKNNKKKVSEGDNLDEDVHEDLDAGFKFDLDADDTTSNFQGWNFLAEGESNKDDAEA
FVKKDVDLDKIIRRKGGLVKMAHIDSKQEEETEKEKVEKENDSDDEELAMDGFGMGAP
MNNGDENQSEEEEEEEEEEEEEEEEEEEEQEEMTLEKGGKDDEIDEEDDSEEAKADFY
APETEGDEAKKQMYENFNSLSLSRPVLKGLASLGYVKPSPIQSATIPIALLGKDIIAG
AVTGSGKTAAFMIPIIERLLYKPAKIASTRVIVLLPTRELAIQVADVGKQIARFVSGI
TFGLAVGGLNLRQQEQMLKSRPDIVIATPGRFIDHIRNSASFNVDSVEILVMDEADRM
LEEGFQDELNEIMGLLPSNRQNLLFSATMNSKIKSLVSLSLKKPVRIMIDPPKKAATK
LTQEFVRIRKRDHLKPALLFNLIRKLDPTGQKRIVVFVARKETAHRLRIIMGLLGMSV
GELHGSLTQEQRLDSVNKFKNLEVPVLICTDLASRGLDIPKIEVVINYDMPKSYEIYL
HRVGRTARAGREGRSVTFVGESSQDRSIVRAAIKSVEENKSLTQGKALGRNVDWVQIE
ETNKLVESMNDTIEDILVEEKEEKEILRAEMQLRKGENMLKHKKEIQARPRRTWFQSE
SDKKNSKVLGALSRNKKVTNSKKRKREEAKADGNGARSYRKTKTDRIADQERTFKKQK
STNSNKKKGFKSRR"
gene complement(118585..120567)
/locus_tag="R008_L10351"
mRNA complement(118585..120567)
/locus_tag="R008_L10351"
/product="hypothetical protein"
CDS complement(118585..120567)
/locus_tag="R008_L10351"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84274.1"
/translation="MKHSRQMPDESLENIKVLLNPKLGKPVKSLMSAQSKACYHTLIS
NKNLNKTSDEYEKLLANYILLCDEKYLCKTVIPDSRFWAILCDNCQKLRSETLVANLI
RIFNVALKCQDSNKNEVIVSICHISRENSQLIGILLQLLSQRPIHIPLFTDTILCITL
FLKCSLTLCETSLSHAVEFVPRILILLFQYNFPASMSELLYIEDLQPLILEEFVPLKQ
RLINFLSSVSIDDYSCSLKADLLTAIKDNSVFQKGLEMEMGDLPSINLLNAYDTFTFL
NSPNGSFKRLYTEQLLFGENDFPLYEAIFKLSDQFRRLFNLSGKKENQYSDSERDLKL
QIATAVLNRQTCFYKTLELFLRFWIESLAKSQSDLVSLLNLAIITLKYVCLSSSDLEA
AIQTKSLLKTQVVALDSMRYKFARTLQLDSIKKEQYRTWSSSIASFDTMLSGQVRDYV
RHQRLLQLQKGTWVYAENPLNPEAGTPKVYFLIVSDNHANLLAREFETQTNDLPYLFD
NKILTSPGSEALANGRTKVVVLKHITSFKSIELTTPSRRTSSNVYIKLDEANVYTGVE
LKDRNDRTVLKFYLDTEEGRYIWLDGLKLISPFQHEDISEDTKEQIDTLFDLRKNVQM
INLNVRQDIIVPPPEPSDEDEDEEFCNLETXXPQVA"
gene 120613..120788
/locus_tag="R008_L10356"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 120853..122133
/gene="Mmm1"
/locus_tag="R008_L10361"
mRNA 120853..122133
/gene="Mmm1"
/locus_tag="R008_L10361"
/product="Mmm1p"
CDS 120853..122133
/gene="Mmm1"
/locus_tag="R008_L10361"
/note="Similar to YLL006W ER integral membrane protein,
component of the ERMES complex that links the ER to
mitochondria and may promote inter-organellar calcium and
phospholipid exchange as well as coordinating
mitochondrial DNA replication and growth"
/codon_start=1
/product="Mmm1p"
/protein_id="EWG84275.1"
/translation="MTDSENESTETDSLMTFDDYISKELPEHLQRLIMENLKGSTTND
LKQTSNNSEFNVSKNGSFKGLDDAIQALQMQSVLHPSSLGSLATSSKFSGWSFAQGFF
VGQLSIVLLFIFFLKFFIFSDEPSKSKNPKPAASRHRSKFKEYPFISREFLTSLVRKG
AKQHYELNEEAENEHLQELALILEKTYYNVDVHPAESLDWFNVLVAQIIQQFRSEAWH
RDNILHSLNDFIGRKSPDLPEYLDTIKITELDTGDDFPIFSNCRIQYSPNSGNKKLEA
KIDIDLNDHLTLGVETKLLLNYPKPGIAALPINLVVSIVRFQACLTVSLTNAEEFAST
SNGSSSENGMEGNSGYFLMFSFSPEYRMEFEIKSLIGSRSKLENIPKIGSVIEYQIKK
WFVERCVEPRFQFVRLPSMWPRSKNTREEKPTEL"
gene complement(122206..124812)
/gene="Spo75"
/locus_tag="R008_L10366"
mRNA complement(122206..124812)
/gene="Spo75"
/locus_tag="R008_L10366"
/product="Spo75p"
CDS complement(122206..124812)
/gene="Spo75"
/locus_tag="R008_L10366"
/note="Similar to YLL005C 'Meiosis-specific protein of
unknown function, required for spore wall formation during
sporulation; dispensable for both nuclear divisions during
meiosis'"
/codon_start=1
/product="Spo75p"
/protein_id="EWG84276.1"
/translation="MNATKELTFNLLNKFQDKERFGSAQRHAGISLKGFISGILFSFL
YFLFQLSLFIILRSRFKTIYQANVVLKIHPGSKVCFAKKKIKNYWSLFAFLKQLPGRM
LDPMEKFERNERYGLDNYLFLRFLKLLIFFFAVLSIINIPILIPIHYFSRDILKENEG
ERYEQSFRTTSKLDKWTMSNLSPNSSNTLICHLFLSIFVVLWFHFILSSELRFVNRLG
YSVLTKSKYQNILYLEGFSSKLVTQSISLETFFQPLHSDCFGVTHFIPKNLKKVHKLE
IKLNKLQKSKEQIIFEIILEKYFRRVSIHRHLIANHKRFFFSKLKNHLLFQYKKLVFL
TQFRISYYCTKIRLRWKKSSIFPLYYPKLYVNTETILERKYRILDKIIRKEKLIKFQV
NSLKATSETKQALPDDLSSGTDIYMDKMFITFKSTLLSNVIGELLSYRLPTQNLKVII
GPNVNDIIWRNILDSSPLWKSAKYFSANILRIFVIIGWILPVAFLGLISQIPNISSLI
PFTKIIHFQSPFIREVAKNLIPIVTLIIIIEIVPYFFRWLSYLRGLKTGAQIEADVQN
WYFVFVFIHLFVVVTISSGFSIIIERLLNNPVSIPALLANDLPKCANFFCSFVLIRGM
AYAGGNLLRIKELLFELFYYKWKRSTPHAQFKRLKTSLFFQLGSIYPIFSVLGCIGII
YSVVAPIILLLCCISFSMVFFSFSYLFKYQYNKENYSETFGKLYIQALMQLYAGIYFM
EFCLLGLFTLFDQYTLSTIMLVVFALTVITHSKISKQIKSKPQRIPTLEYLSNLTEEK
KDQFCQESYSFHDIFSICRNSDEIWLPRDKLGISEEEQSFLEKSYHLKFDLNMYSMNL
FGDCHLENSHLH"
gene 125338..127188
/gene="Orc3"
/locus_tag="R008_L10371"
mRNA 125338..127188
/gene="Orc3"
/locus_tag="R008_L10371"
/product="Orc3p"
CDS 125338..127188
/gene="Orc3"
/locus_tag="R008_L10371"
/note="Similar to YLL004W Subunit of the origin
recognition complex, which directs DNA replication by
binding to replication origins and is also involved in
transcriptional silencing"
/codon_start=1
/product="Orc3p"
/protein_id="EWG84277.1"
/translation="MSDLNQSKKMNVSEFADAQRSHYTVYPSLPQSNKNDKHIPFVKL
LSGKESEVNVEKRWELYHQLHSHFHDQVDHIIDNIEADLKAEISDLLYSETTQKRRCF
NTIFLLGSDSTTKIELKDESSRYNVLIELTPKESPNVRMMLRRSMYKLYSAADAEEHP
TIKYEDINDEDGDFTEQNNDVSYDLSLVENFKRLFGKDLAMVFNFKDVDSINFNTLDN
FIILLKSAFKYDHVKISLIFNINTNLSNIEKNLRQSTIRLLKRNYHKLDVSSNKGFKY
GNQIFQSFLDTVDGKLNLSDRFVEFILSKMANNTNHNLQLLTKMLDYSLMSYFFQNAF
SVFIDPVNVDFLNDDYLKILSRCPTFMFFVEGLIKQHAPADEILSLLTNKNRGLEEFF
VEFLVRENPINGHAKFVARFLEEELNITNFNLIELYHNLLIGKLDSYLDRWSACKEYK
DRLHFEPIDTIFQELFTLDNRSGLLTQSIFPSYKSNIEDNLLSWEQVLPSLDKENYDT
LSGDLDKIMAPVLGQLFKLYREANMTINIYDFYIAFRETLPKEEILNFIRKDPSNTKL
LELAETPDAFDKVALILFMQAIFAFENMGLIKFQSTKSYDLVEKCVWRGI"
gene 127466..130306
/gene="Sfi1"
/locus_tag="R008_L10376"
mRNA 127466..130306
/gene="Sfi1"
/locus_tag="R008_L10376"
/product="Sfi1p"
CDS 127466..130306
/gene="Sfi1"
/locus_tag="R008_L10376"
/note="Similar to YLL003W Centrin (Cdc31p)-binding protein
required for spindle pole body (SPB) duplication,
localizes to the half-bridge of the SPB, required for
progression through G(2)-M transition, has similarity to
Xenopus laevis XCAP-C"
/codon_start=1
/product="Sfi1p"
/protein_id="EWG84278.1"
/translation="MGKFGTTNKSTENLLRDKFVPETSPTNIPTDVLIKQGQITDSTE
SLIHGGAERYIVNALKPIELNKTEGFFEDPPFHLPSPPVDSTNLEYEDVTDLPKNGLR
YDLNDISVEVMEDLYRQIEAFLVHFKLSRSFLQIFKNYVNILIQEGINPLRDEYFTIL
EDELKGFFTFNSVIEEILEIFLIHPRNKFIALSLAEYTYAKNKIRRHFNHWKTVCELN
EEANRFANQAKLRVQEAVFYIWSDKTLKYSQMANDEAESFRNTWLLFRSFQQWITLTQ
TLKEQSRLADQAFLNKMFRKILKAQEHWKHLETVNTDNIKKIFLRTTFHIWKLRHKEI
NYHGLERRIFERIKQKVINYEYNKSIAEKVRSFSLQRKYLNKWEKKNIENEDKLGALY
ELENKFIKQKFFRKLNRSFQHSQQEAIAKSKLNQTLLRCVFEKMWLKRFEDHLHLYSI
VSLKEANLVKRIFHSWKKLLYIDLKASDYSRTNLLKSSLRSWKLEVKLKIFEQKCKKS
IQASAYRTWRKRIQYGKISSEHVKTAFCAKYLDVWKRRMLQMNSMNDEASKFYEEGLV
NECLAIWKERLIKTKELEDRYNFLCKTHAILTVKRTLMHIDNVHLLYTKLAPSMDRIK
LSKAFLKWRKATRFKVRHKLNDILHVYEKSKERELQSQLFNAWRNRFCFYTEECNIQA
ISKRNYQLEKMVLKKFRERLLEIVKSEELADEVREEFVLVKTFYIWKTHLDEIFYMST
LLEQSEANKQFIITSKFLKMWSLRFLKIKRNDETVEVFRHRWDRATVRGLLLLWKNRS
DSSPKRRKDFNLKHELKTPIRSDSQNASTIPGSERIKQHRMEAMKSHYSRARRAIPSP
VKSSSVLDSTAKKQINLESTTGLNGSPTRGKPLRYSPRRTTRNMPSKVDHIDFGRIPA
VPFSLSANSPKIDQDMDYIREHDKSPLSRKRQ"
gene 130556..131866
/gene="Rtt109"
/locus_tag="R008_L10381"
mRNA 130556..131866
/gene="Rtt109"
/locus_tag="R008_L10381"
/product="Rtt109p"
CDS 130556..131866
/gene="Rtt109"
/locus_tag="R008_L10381"
/note="Similar to YLL002W 'Histone acetyltransferase
critical for cell survival in the presence of DNA damage
during S phase; acetylates H3-K56 and H3-K9; involved in
non-homologous end joining and in regulation of Ty1
transposition; interacts physically with Vps75p'"
/codon_start=1
/product="Rtt109p"
/protein_id="EWG84279.1"
/translation="MSLNDFLSSVLPVSEQFEYLSLQSIPLETHAVVTPNKDDKRVPK
STIKTQHFFSLFHQGKVFFSLEVYVYVTLWDEADAERLIFVSKADTNGYCNTRVSVRD
ITKIILEFILSIDPNYYLQKVKPAIRSYKKISPELISAASTPARTLRILARRLKQSGS
TVLKEIESPRFQQDLYLSFTCPREILTKICLFTRPASQYLFPDSSKNSKKHILNGEEL
MKWWGFILDRLLIECFQNDTQAKLRIPGEDPARVRSYLRGMKYPLWQVGDIFTSKENS
LAVYNIPLFPDDPKARFIHQLAEEDRLLKVSLSSFWIELQERQEFKLSVTSSVMGISG
YSLATPSLFPSSADVIVPKSRKQFRAIKKYITGEEYDTEEGAIEAFTNIRDFLLLRMA
TNLQSLTGKREHRERNQPVPASNINTLAITMLKPRKKAKALPKT"
gene 132155..134428
/gene="Dnm1"
/locus_tag="R008_L10386"
mRNA 132155..134428
/gene="Dnm1"
/locus_tag="R008_L10386"
/product="Dnm1p"
CDS 132155..134428
/gene="Dnm1"
/locus_tag="R008_L10386"
/note="Similar to YLL001W 'Dynamin-related GTPase required
for mitochondrial fission and morphology; assembles on the
cytoplasmic face of mitochondrial tubules at sites at
which division will occur; also participates in
endocytosis and regulating peroxisome abundance'"
/codon_start=1
/product="Dnm1p"
/protein_id="EWG84280.1"
/translation="MASLEDLIPTVNKLQDVMYDSGIDTLDLPILAVVGSQSSGKSSI
LETLVGRDFLPRGTGIVTRRPLVLQLNNISPNSPLIEEDDNSVNPHDEVTKISGFEAG
TKPLEYRGKERNHADEWGEFLHIPGKRFYDFDDIKREIENETARIAGKDKGISKIPIN
LKVFSPHVLNLTLVDLPGITKVPIGEQPPDIEKQIKNLILDYIATPNCLILAVSPANV
DLVNSESLKLAREVDPQGKRTIGVITKLDLMDSGTNALDILSGKMYPLKLGFVGVVNR
SQQDIQLNKTVEESLDKEEDYFRKHPVYRTISTKCGTRYLAKLLNQTLLSHIRDKLPD
IKTKLNTLISQTEQELARYGGVGATTNESRASLVLQLMNKFSTNFISSIDGTSSDINT
KELCGGARIYYIYNNVFGNSLKSIDPTSNLSVLDVRTAIRNSTGPRPTLFVPELAFDL
LVKPQIKLLLEPSQRCVELVYEELMKICHKCGSAELARYPKLKSMLIEVISELLRERL
QPTRSYVESLIDIHRAYINTNHPNFLSATEAMDDIMKTRRKRNQELLKSKLSQQENGQ
TNGINGTSSISSNIDQDSAKNSDYDDDGIDAESKQTKDKFLNYFFGKDKKGQPVFDAS
DKKRSIAGDGNIEDFRNLQISDFSLGDIDDLENAEPPLTEREELECELIKRLIVSYFD
IIREMIEDQVPKAVMCLLVNYCKDSVQNRLVTKLYKETLFEELLVEDQTLAQDRELCV
KSLGVYKKAATLISNIL"
gene complement(135897..138495)
/locus_tag="R008_L10396"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(138861..140852)
/gene="Noc3"
/locus_tag="R008_L10401"
mRNA complement(138861..140852)
/gene="Noc3"
/locus_tag="R008_L10401"
/product="Noc3p"
CDS complement(138861..140852)
/gene="Noc3"
/locus_tag="R008_L10401"
/note="Similar to YLR002C 'Protein that forms a nuclear
complex with Noc2p that binds to 66S ribosomal precursors
to mediate their intranuclear transport; also binds to
chromatin to promote the association of DNA replication
factors and replication initiation'"
/codon_start=1
/product="Noc3p"
/protein_id="EWG84281.1"
/translation="MAKRNRSQFRIQERTAKKRKHEDSLLEGNVFQNAPEDMDENTIY
SAKGSSWDEEEQDYEMVPRKNRSDTSNLVEGLPIKVNGNVERKLHKAQEKPKDDDEED
EDSNDSSEDDEGPNEEQEAEAKEDEPDTEEKILQLKEDIADLVTKVMEEPEENTAALG
RLCKMVESKNPNTCKFSMLALVPVFKSIIPGYRIRPLTETEKKEKVSKEVSKLRNFEQ
ALVYNYKNYVGRLQSLSKTPSNAAPIQVSLGILATQAAKELISTASHFNFRTDIFTLL
LRRICKPRISTDPTSIQIIQTFETLLNEDEEGSISFEILRIFNKILKTRNFNIEESVL
NMLLSLDVLHDYDPNTKSKGNVSAPKLKKKDRVHLSKKQRKARKEMQQIEEEMRNAEQ
AVSAEERERNQSEILKIVFTIYLNILKNNAKTLIGSVLEGLTKFGNMANFDLLGDFLE
VMKELISDTEFDNLSSAEVRKALLCIVSAFSLISNTQYMKVNVDLSKFVDGLYALLPY
ICLDADIELSYRSLRLADPLNNEIIKPSVNVSTKAELLLKALDHVFFRSKSGTKERAT
AFTKRLYMCISHTPEKTSIAILKFIDKLMNRYPEISGLYSSEDRIGNGHFIMEADNPS
RSNPEAATLWDNALLEKHYCPVVTKGLRSLSSRSKECSK"
gene complement(141373..142248)
/locus_tag="R008_L10406"
mRNA complement(141373..142248)
/locus_tag="R008_L10406"
/product="hypothetical protein"
CDS complement(141373..142248)
/locus_tag="R008_L10406"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84282.1"
/translation="MSNPDDLDDGLAYDFDAEHEVIFDAKDGSPPTKKVQKRSIEQDD
DDVDDIDGKKEERNSEDDSNRPISKRQKKLQKKSKLIEKKKEESQYIVSQRKALPASS
PEKIIEYLTTLIREKNPDLSVLELEELYFKRNDFLSTEKFDAERRLSNFPAFIQKFSV
APKKIVFSMSNIRVADVYRSLNGGKNCVKLFSKSKLKDDIATVERLLTDSSKKSNKNK
DSLYFIATPTRMQKIIEATDLLFQGKEKLDIILDASYLDPKDNTILSFENAAVLCQVL
KTFLNKKSSVKILLY"
gene complement(142452..144023)
/gene="Thi73"
/locus_tag="R008_L10411"
mRNA complement(142452..144023)
/gene="Thi73"
/locus_tag="R008_L10411"
/product="Thi73p"
CDS complement(142452..144023)
/gene="Thi73"
/locus_tag="R008_L10411"
/note="Similar to YLR004C 'Putative plasma membrane
permease proposed to be involved in carboxylic acid uptake
and repressed by thiamine; substrate of Dbf2p/Mob1p
kinase; transcription is altered if mitochondrial
dysfunction occurs'"
/codon_start=1
/product="Thi73p"
/protein_id="EWG84283.1"
/translation="MKNMSQRSMDVEKKAANADSCSVSTSSINVDDADVALRFLKQNG
LDESSTANEDDVVAGEEANFYGSHELSPKVLRKVDLFILPFLCCTYLLMFLDKALLNY
AASMGIKDHLKGNEFSNLGTIFSAAYIFMEPVVTYLIQKFPISKILGTFITVWGIVLA
CHAACKTYASLMVVRTLLGLFESSSAVGCIAISGMYYTKSEQSARIGFWATQAGTGYI
VGGLISFGFLHYHGTAFTSWQIMFLVVGLVTVAFGVLTFLYLPDNVTNAWFLNKEEKI
QVVEHIRANQTGLETKKFKKQQVKELFLHDKFTWPMLLLTACSQISTGAIGTFSVTIT
GTFGFDKYETALLQLPIGAITAMIILITTQMLSRWGHITLITTSMYIPAIIGCIVLIS
LPLSHKIGNLFSLYLLYSGSCVITNIYIWNSCNTSGYTKRVFRNAITMIVYNVSCIIA
PQMFRAYSAPRYIPAKIALLVTQCVCVPLQLYIGYICKKENEKRDKEQEGQERKKYQF
LDLTDIENRNFRYIY"
gene 144567..145952
/gene="Ssl1"
/locus_tag="R008_L10421"
mRNA 144567..145952
/gene="Ssl1"
/locus_tag="R008_L10421"
/product="Ssl1p"
CDS 144567..145952
/gene="Ssl1"
/locus_tag="R008_L10421"
/note="Similar to YLR005W 'Component of the core form of
RNA polymerase transcription factor TFIIH, which has both
protein kinase and DNA-dependent ATPase/helicase
activities and is essential for transcription and
nucleotide excision repair; interacts with Tfb4p'"
/codon_start=1
/product="Ssl1p"
/protein_id="EWG84284.1"
/translation="MAPVVISESEEDEDRVAITRRTKRQVHFDGEGDDRVDQQQQQHS
SSHRDRDKHVQRKKKKRLSNRNLQGSNGGYAWEDEIKRSWDLVKVDDEGDMASLVASI
VEARKKRTAKKNITPYQRGIIRSLILTLDCSEAMLEKDLRPNRHAMIIQYAIDFVHEF
FDQNPISQMGIIIMRNGLAQLVSQVSGNPQDHIDALKSIRKQEPKGNPSLQNALEMAR
GLLLPVPAHCTREVLIVFGSLSTTDPGDIHQTIDSLVSEKIRVKVLGLSAQVAICKEL
CKATNYGDESFYKILLDETHLKELFNEAVTPLPVNKINKGFTLVKMGFPTRIFEDTPT
FCSCHSKLVYGGYFCPNCHSKVCSLPTVCPCCDLMLILSTHLARSYHHLMPLKTFAEV
PTTEKFRSEDCFSCQSRFPILKNHKNGKLLTSSRYRCEDCKQEFCVDCDVFIHEILHN
CPGCESKPVIT"
gene complement(146244..148433)
/gene="Ssk1"
/locus_tag="R008_L10426"
mRNA complement(146244..148433)
/gene="Ssk1"
/locus_tag="R008_L10426"
/product="Ssk1p"
CDS complement(146244..148433)
/gene="Ssk1"
/locus_tag="R008_L10426"
/note="Similar to YLR006C 'Cytoplasmic response regulator;
part of a two-component signal transducer that mediates
osmosensing via a phosphorelay mechanism; required for
mitophagy; dephosphorylated form is degraded by the
ubiquitin-proteasome system; potential Cdc28p substrate'"
/codon_start=1
/product="Ssk1p"
/protein_id="EWG84285.1"
/translation="MLNSALLWKVWLRIDNSTDEVNQPIAVQFDEIDTVDDLKSRFFQ
KLSSTRWREINDNASIAIGLYAPKFDNQADNTSSNNTNDNSCRSKSNGAGSGANLSVN
SNTKSSVSPTAGSFGLSKDLAKDRNVLQHPKPTQKRGALYDAFAAVPTVAATTNVDFP
PNEAPMLSPQRPYSTSPKQFPATTKSPLLRFASVSPYPKFHSDNQIMASAGLTYVSPH
NKNKYTRPLIRKGLNFTTESVNDCTYKIIFEPDELAINIYKELFGTMGSQPASQPLLI
FSNVNLRQDVPPLDILNVVDYVPTNEEISQQKTQPTDHGAVGVFHLDDHISPGEQGLK
QTIGDKADLKGKDGNSSPQEFKLITDEEQLRRASQELKDEEKDAESPWQAILLLPKGY
KGGVDFRNKPVAHTDSSFNNEDTITHSELEVNTGSPSQESGSLNEAGIGITQPMSEVQ
RRKEDVTPASPILTSSQTPHYSNSLYNAPFAVSSPPDPLPNLFTTTSEKVFPKINVLI
VEDNVINQAILGSFLRKHKISYKLAKNGQEAVNIWKEGGLHLIFMDLQLPVLSGIEAA
KQIRDFEKQNGIGIQKSLNNSHSNLEKGTSKRFSQAPVIIVALTASNSQMDKRKALLS
GCNDYLTKPVNLHWLSKKITEWGCMQALIDFDSWKQGESRMTDSVLVKSPQKPIAPSN
PHSFKQATSMTPTHSPVRKNSNLSPTQIELNRIVSLVVPCRGHYDRM"
gene 148932..149942
/gene="Nse1"
/locus_tag="R008_L10431"
mRNA 148932..149942
/gene="Nse1"
/locus_tag="R008_L10431"
/product="Nse1p"
CDS 148932..149942
/gene="Nse1"
/locus_tag="R008_L10431"
/note="Similar to YLR007W 'Essential subunit of the
Mms21-Smc5-Smc6 complex; nuclear protein required for DNA
repair and growth; has a nonstructural role in the
maintenance of chromosomes'"
/codon_start=1
/product="Nse1p"
/protein_id="EWG84286.1"
/translation="MEVHEEQVSAPVTGDATAKYLLQYILSARGICHENALILALMRL
ETDASTLNTEWSIQQWVDKLNDYINAINVKLNLLGYKIIRINHGIGRNAVTLKAKQNF
ESFEDNTAIRAHNNDYAVLQSIVLPESNRFFVYVNLASTEETKLATRFNQNEIEFMKW
AIEQFMISGETIVEGPALETSIIVKEVNRILVAATGDSNLAKWRKFSTFTVGSTNLFQ
FQELTATDIEDLLLRLCELKWFYRTQEGKFGIDLRCIAELEEYLTSMYNLNTCQNCHK
LAIQGVRCGNESCREENEETGENSLSQIWHVDCFKHYITHVSKNCDRCGSSLITEGVY
VI"
gene complement(150117..150623)
/gene="Pam18"
/locus_tag="R008_L10436"
mRNA complement(150117..150623)
/gene="Pam18"
/locus_tag="R008_L10436"
/product="Pam18p"
CDS complement(150117..150623)
/gene="Pam18"
/locus_tag="R008_L10436"
/note="Similar to YLR008C 'Constituent of the import motor
(PAM complex) component of the Translocase of the Inner
Mitochondrial membrane (TIM23 complex); essential
J-protein cochaperone that stimulates Ssc1p ATPase
activity to drive import; inhibited by Pam16p'"
/codon_start=1
/product="Pam18p"
/protein_id="EWG84287.1"
/translation="MSSQSNTGNSIEAPQLPIPGQTNGSANVTVDGAGVNVGIQNGSQ
GQKTGMDLYFDQALNYMGEHPVITGFGAFLTLYFTAGAYKSISKGLNGGKSTTAFLKG
GFDPKMNSKEALQILNLTENTLTKKKLKEVHRKIMLANHPDKGGSPFLATKINEAKDF
LEKRGISK"
gene 151077..151676
/gene="Rlp24"
/locus_tag="R008_L10441"
mRNA 151077..151676
/gene="Rlp24"
/locus_tag="R008_L10441"
/product="Rlp24p"
CDS 151077..151676
/gene="Rlp24"
/locus_tag="R008_L10441"
/note="Similar to YLR009W Essential protein with
similarity to Rpl24Ap and Rpl24Bp, associated with pre-60S
ribosomal subunits and required for ribosomal large
subunit biogenesis"
/codon_start=1
/product="Rlp24p"
/protein_id="EWG84288.1"
/translation="MRIYQCHFCSSPCYPGHGIMFVRNDAKEFRFCRSKCHKAFKQRR
NPRKLKWTKAFRKAAGKELAVDSTLTFAQRRNVPVRYNRELVATTLKAMARIEEIRQK
RERAFYKNRMRGNKEKDFLRDKKLVESNPELLRIREVEIARKLAKEQERAESVSEQEE
SEEEEEDMEIDSDEEEEEQLEKQKILLKNRRRNTKKIAF"
gene complement(151860..152252)
/gene="Ten1"
/locus_tag="R008_L10446"
mRNA complement(151860..152252)
/gene="Ten1"
/locus_tag="R008_L10446"
/product="Ten1p"
CDS complement(151860..152252)
/gene="Ten1"
/locus_tag="R008_L10446"
/note="Similar to YLR010C 'Protein that regulates
telomeric length; protects telomeric ends in a complex
with Cdc13p and Stn1p'"
/codon_start=1
/product="Ten1p"
/protein_id="EWG84289.1"
/translation="MEPNIIHPSKRFRIVVRLVDFLFCKSDEEFIKGFFCQMIVRNLH
CLNSTNGAEEMRLYMSERLFSAHKDDLRLINGQVLDVRIGVWYGIHQSPPIFEIIDFK
ILSRNDVRDFCEFVKSPLGEKFLNISNS"
assembly_gap 152299..152827
/estimated_length=529
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 153628..154203
/gene="Lot6"
/locus_tag="R008_L10451"
mRNA 153628..154203
/gene="Lot6"
/locus_tag="R008_L10451"
/product="Lot6p"
CDS 153628..154203
/gene="Lot6"
/locus_tag="R008_L10451"
/note="Similar to YLR011W 'FMN-dependent NAD(P)H:quinone
reductase, may be involved in quinone detoxification;
expression elevated at low temperature; sequesters the
Cin5p transcription factor in the cytoplasm in complex
with the proteasome under reducing conditions'"
/codon_start=1
/product="Lot6p"
/protein_id="EWG84290.1"
/translation="MKVGIIMGSVRAKRVCPEIAAYVKRTIENSEELIDQKLKIQVVD
LQQIALPLYEDDDELIPAQIKSVDEYADSKTRSWSRIVNALDIIVFVTPQYNWGYPAA
LKNAIDRLYHEWHGKPALVVSYGGHGGSKCNDQLQEVLHGLKMNVIGGVAVKIPVGTI
PLPEDIVPQLSVHNEEILQLLASCIETTRNK"
gene complement(154507..154806)
/locus_tag="R008_L10456"
mRNA complement(154507..154806)
/locus_tag="R008_L10456"
/product="hypothetical protein"
CDS complement(154507..154806)
/locus_tag="R008_L10456"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84291.1"
/translation="MKTVYYKEITYQQYLQLQPEQQEKYLALCQKDFEQETERIAFDR
QGGVPGIARKFAQEEVAWFDRVTTWSYMNAYIPSYRRRRNLLKIDMLKMSNAEEY"
gene 155874..156299
/gene="Gat3"
/locus_tag="R008_L10461"
mRNA 155874..156299
/gene="Gat3"
/locus_tag="R008_L10461"
/product="Gat3p"
CDS 155874..156299
/gene="Gat3"
/locus_tag="R008_L10461"
/note="Similar to YLR013W Protein containing GATA family
zinc finger motifs"
/codon_start=1
/product="Gat3p"
/protein_id="EWG84292.1"
/translation="MNIKTLCHPEYKRISVESLLNPVEETIDCEKPHSQTKINTAKPI
SASLYVTTNNTAVVQHNVQKRKGVTRRCPQCAVIKTSPQWREGPDGEVTLCNACGLFY
RKIFLVFGKDLAKRYFNEIKGVSVKQKVPKSLYGVTRTR"
gene complement(156813..159527)
/gene="Ppr1"
/locus_tag="R008_L10466"
mRNA complement(156813..159527)
/gene="Ppr1"
/locus_tag="R008_L10466"
/product="Ppr1p"
CDS complement(156813..159527)
/gene="Ppr1"
/locus_tag="R008_L10466"
/note="Similar to YLR014C 'Zinc finger transcription
factor containing a Zn(2)-Cys(6) binuclear cluster domain,
positively regulates transcription of genes involved in
uracil biosynthesis; activity may be modulated by
interaction with Tup1p'"
/codon_start=1
/product="Ppr1p"
/protein_id="EWG84293.1"
/translation="MKQKKFNSKKSNRTDLSKRGDSPNIGISKSRTACKRCRLKKIKC
DQEFPSCKRCAKLEVPCVSLDPATGKDVPRSYVFFLEDRLAVMMRVLKEYGVDPTKIR
GNIPATSDDEPFDLKKYSSVSSLGEEGILPHNGLLADYLVQKGNSMASSAITSKSMAS
PQTINVQRKEFLVNSKKQDGSALLPETGSPMTSDARAEELRRCNKEISALGTMRESSF
NSFLGDSSGISFAKLVFTATNFRQDSGDDVLDEDIKQREQKYNGYAEAENNPHFDPLE
LPPRHAAEVMISRFFVDTNSQLPLLHRELFLKKYFEPIYGPWNPNIALASDQTGINSA
FEIPITSAFSAHTEPKRENVTEKIDVCSSEDVPWYDTWETSQKVNMRPIVELPTKFHI
PYFFLNIIFAIGHATQVLKSDITTVATYKRRATKYIASLFSSSDRLEALAGTLLMVIY
SIMRPNVPGVWYTMGSVLRLTVDLGLHSEKINKNYDAFTREIRRRLFWCVYSLDRQIC
SYFGRPFGIPEESITTRYPSLLDDSFITLTNREIDDYSDLPSPNPSSKVIALAMYKIR
RIQASIVRILYAPGAELPRRFMDLESWRIETYNELERWFQVDVPKNFEMMNCKFNSIW
FDLNYHYSKSILYGLSPKFPTLNDTAFKIVLDSTKGTIDVFYNLCVNKKIGYTWVAVH
NMFMTGMTYLYVNFYSKNNINDCQEKVSEYTEKVLIVLKNLIGFCESAKTCYTSYKIL
SSVVIKLKFMQINDAKGIFSDSNPLTSQANRMSSYDKKTNVLGFDDGTFDNKVFNRTN
FEEKAPFDIPLDEFFTELEKHSNVSQFNTLDVSEGNQVINESASTNTSSALNCQSYTN
NQDIMDILFQVTSGSVWDEFFVRSGNGNEGESSYDISKGKNSESGGIF"
gene 159772..161289
/gene="Bre2"
/locus_tag="R008_L10471"
mRNA 159772..161289
/gene="Bre2"
/locus_tag="R008_L10471"
/product="Bre2p"
CDS 159772..161289
/gene="Bre2"
/locus_tag="R008_L10471"
/note="Similar to YLR015W 'Subunit of COMPASS (Set1C)
complex, which methylates Lys4 of histone H3 and functions
in silencing at telomeres; has a C-terminal Sdc1 Dpy-30
Interaction (SDI) domain that mediates binding to Sdc1p;
similar to trithorax-group protein ASH2L'"
/codon_start=1
/product="Bre2p"
/protein_id="EWG84294.1"
/translation="MKLGIIPYQEGTDIVYKNALQGQQEGKRPNLPQMEATHQIKSSV
QGTSYEFVRTEDIPLNRRHFVYRPCSANPFFTILGYGCTEYPFDHSGMSVMDRSEGLS
ISRDGNDLVSVPDQYGWRTARSDVCIKEGMTYWEVEVIRGGNKKFADGVNNKENADDS
VDEVQSGIYEKMHKQVNDTPHLRFGVCRREASLEAPVGFDVYGYGIRDISLESIHEGK
LNCVLENGSPLKEGDKIGFLLSLPSIHTQIKQAKEFTKRRIFALNSHMDTMNEPWRED
AENGPSRKKLKQETTNKEFQRALLEDIEYNDVVRDQIAIRYKNQLFFEATDYVKTTKP
EYYSSDKRERQDYYQLEDSYLAIFQNGKYLGKAFENLKPLLPPFSELQYNEKFYLGYW
QHGEARDESNDKNTTSAKKKKQQQKKKKGLILRNKYVNNNKLGYYPTISCFNGGTARI
ISEEDKLEYLDQIRSAYCVDGNSKVNTLDTLYKEQIAEDIVWDIIDELEQIALQQ"
gene complement(161347..161961)
/gene="Pml1"
/locus_tag="R008_L10476"
mRNA complement(161347..161961)
/gene="Pml1"
/locus_tag="R008_L10476"
/product="Pml1p"
CDS complement(161347..161961)
/gene="Pml1"
/locus_tag="R008_L10476"
/note="Similar to YLR016C 'Subunit of the RES complex,
which is required for nuclear retention of unspliced
pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p'"
/codon_start=1
/product="Pml1p"
/protein_id="EWG84295.1"
/translation="MFHRRKRPYNTRNYGHDDKKFKSQYIDIMPDFSPSGLLELESNN
KEGIALKHVEPQDAISPDNYMDMLGLEARDRTMYELVIYRKNDKDKGPWKRYDLNGRS
CYLVGRELGHSLDTDLDDRTEIVVADIGIPEETSSKQHCVIQFRNVRGILKCYVMDLD
SSNGTCLNNVVIPGARYIELRSGDVLTLSEFEEDNDYELIFMNV"
gene 162152..163165
/gene="Meu1"
/locus_tag="R008_L10481"
mRNA 162152..163165
/gene="Meu1"
/locus_tag="R008_L10481"
/product="Meu1p"
CDS 162152..163165
/gene="Meu1"
/locus_tag="R008_L10481"
/note="Similar to YLR017W 'Methylthioadenosine
phosphorylase (MTAP), catalyzes the initial step in the
methionine salvage pathway; affects polyamine biosynthesis
through regulation of ornithine decarboxylase (Spe1p)
activity; regulates ADH2 gene expression'"
/codon_start=1
/product="Meu1p"
/protein_id="EWG84296.1"
/translation="MNRIKNTFSVAKRLKLSKVMTNSELPSIFEGTVDLGIIGGTGLY
NLDCLEPIALLPPMVTPWGTTSSPVTISQFVGTNSHFHVAFIARHGINHEYPPTKVPF
RANMAALKNLNCKAVLSFSAVGSLQPHIKPRDFVLPQQIIDRTKGIRHSSYFNDEGLV
GHVGFGQPFSQKFAEYIYQFKNEITNPESEEPCHLHYDKDMTVVCMEGPQFSTRAESK
MYRMFGGHVINMSVIPEAKLARECELPYQMICMSTDYDAWRDEAEPVTVETVIGNLTN
NGRNANILASKIIVSMAKEIPEFMHTGDGLRGSIKKSISTKPEAMSKETLERLRYLFP
NYW"
gene complement(163251..164150)
/gene="Pom34"
/locus_tag="R008_L10486"
mRNA complement(163251..164150)
/gene="Pom34"
/locus_tag="R008_L10486"
/product="Pom34p"
CDS complement(163251..164150)
/gene="Pom34"
/locus_tag="R008_L10486"
/note="Similar to YLR018C 'Integral membrane protein of
the nuclear pore; has an important role in maintaining the
architecture of the pore complex'"
/codon_start=1
/product="Pom34p"
/protein_id="EWG84297.1"
/translation="MKIQAGQLGLDDNDVPGPLPDTDSKPSSQSQNDTPMFKLGNFES
PVLKELSRRTVNKEMETQRIMTNVIAFAFWNLLVKFIKFFWNNTHVGRQFCNRLSRIH
LYMLTFHTLKKANIIYHTTFSWLNAELLDYLFHLLISLNILFSLWKLLSTVKVSDLNL
TDRQKKLLGVDMQSSVDTGLQPQHPHYVSTSKISQMAQNKTHIPQTNLKNHPAYLFKG
LETPLKARQREMAEEQTKLQSQSLHTKNVFGTLQRHSGISSTLVSANNDNNSPHTPVT
RKGYIPSSKYAYMMNSQSPRGKI"
gene 164856..166049
/gene="Psr2"
/locus_tag="R008_L10491"
mRNA 164856..166049
/gene="Psr2"
/locus_tag="R008_L10491"
/product="Psr2p"
CDS 164856..166049
/gene="Psr2"
/locus_tag="R008_L10491"
/note="Similar to YLR019W 'Functionally redundant Psr1p
homolog, a plasma membrane phosphatase involved in the
general stress response; required with Psr1p and Whi2p for
full activation of STRE-mediated gene expression, possibly
through dephosphorylation of Msn2p'"
/codon_start=1
/product="Psr2p"
/protein_id="EWG84298.1"
/translation="MGFIANILCCSSDTSKTHRQRQPPETNHNRNRNRKHSSNKAQAQ
GRKQKATPNGDKMQYSTPEILLSSSDSGSNAGSKTMQENGNSGNGKLAPLSRDHSNNS
YDEEKEYEDYNEGDVEMTEVNNAGEEEEEDDEAKEKQDHVVHEYNVDADRNSSINDEA
PPQQGLYQVGQEDMNPQYVASSPDNDLNLIPTTEEDFSDLTHLQPDQYHAPGYDTLLP
PKLQEFQQKKCLILDLDETLVHSSFKYMHSADFVLPVEIDDQVHNVYVIKRPGVDEFL
NRVSQLYEVVVFTASVSRYANPLLDTLDPNGTIHHRLFREACYNYEGNYIKNLSQIGR
PLSETIILDNSPASYIFHPQHAVPISSWFSDTHDNELLDIIPLLEDLSSGNVLDVGSV
LDVTI"
gene complement(166357..167973)
/gene="Yeh2"
/locus_tag="R008_L10496"
mRNA complement(166357..167973)
/gene="Yeh2"
/locus_tag="R008_L10496"
/product="Yeh2p"
CDS complement(166357..167973)
/gene="Yeh2"
/locus_tag="R008_L10496"
/note="Similar to YLR020C 'Steryl ester hydrolase,
catalyzes steryl ester hydrolysis at the plasma membrane;
involved in sterol metabolism'"
/codon_start=1
/product="Yeh2p"
/protein_id="EWG84299.1"
/translation="MVNKVVDEVQRLVSAIILTSFMTGLFILSLWKNYVTVHFQHKND
PRDTRSSRTKIQPNDKKKKRPARHSRPLSISSTTPLDLQRDQENNIEYDRTVTSKLSM
TSNTSLSENGDGNANIKMETNVNQAPYAAENPFQNIALAEDTKLVPDLKYYYKEYGID
IEEFEVETDDGFIIDLWHFKSRLNDGVEEVKREPILLLHGLLQSCGAFASSGRKSLAY
FLYESGFDVWLGNNRCGLNAKWNMKKLGNDHSKKWDWDMHQMVQYDLKALINYVLDST
GYAKLSLVAHSQGTTQGFMGLVNGEKLYASDFKLVDKLENFVALAPAVYPGPLLDEKA
FVRLMAKGIDSPWYFGRRSFIPLMMTMRKLMVGTKIFSFLSYIMFNYLFDWNDVLWDR
VLRDRNFLFSPVHISVKLMQWWLSPLPNKLSFKKGAEKIFPDKKTWFPIAKNDDDSGN
NLDNNKLHLNPKRQNSEEFPHIIMFIPKQDRLVDGERLINHFINHEANAVYKIWYIDE
YSHLDVLWAHDVIDRIGKPMIENLRFPNAR"
gene 168191..168730
/gene="Irc25"
/locus_tag="R008_L10501"
mRNA 168191..168730
/gene="Irc25"
/locus_tag="R008_L10501"
/product="Irc25p"
CDS 168191..168730
/gene="Irc25"
/locus_tag="R008_L10501"
/note="Similar to YLR021W 'Component of a heterodimeric
Poc4p-Irc25p chaperone involved in assembly of alpha
subunits into the 20S proteasome; may regulate formation
of proteasome isoforms with alternative subunits under
different conditions'"
/codon_start=1
/product="Irc25p"
/protein_id="EWG84300.1"
/translation="MISYEFQTHLPKGKDSSLNASSENKELYVQATHFNNTILLQIRL
NGEMDSTYEVSSKGLNPILDINVPLAGNLGNTGGDYDDEEEEFVRDHLSDYQVVTKLG
DSADPKVPVVCVQIAELYRRVILPEVSGTMAQDNMQFSLLISMSSKIWRATKEQSADD
NDFGKLVFVLNCIKDMYAK"
gene complement(168742..169494)
/gene="Sdo1"
/locus_tag="R008_L10506"
mRNA complement(168742..169494)
/gene="Sdo1"
/locus_tag="R008_L10506"
/product="Sdo1p"
CDS complement(168742..169494)
/gene="Sdo1"
/locus_tag="R008_L10506"
/note="Similar to YLR022C 'Essential protein involved in
60S ribosome maturation; ortholog of the human protein
(SBDS) responsible for autosomal recessive
Shwachman-Bodian-Diamond Syndrome; highly conserved across
archae and eukaryotes'"
/codon_start=1
/product="Sdo1p"
/protein_id="EWG84301.1"
/translation="MPINQPSGQIKLTNVSLVRLKKARKRFEVACYQNKVQDYRKGIE
KDLDEVLQIHQVFMNVSKGLVANKEDLQKCFGTTNVDDVIEEIMHKGEIQLSEKERQL
MLNKVNNEMLTIVSAKCINPVSKKRYPPTMIHKALQELKFSPVINKPAKLQALEAIKL
LVSKQIIPIVRAKMKVKVAISEPSRQPELIEKISKLIASSPGESTKPELDPWTCTGLI
DPVNYRDLMTLCDKKGTVQVLDMAVIDNTTHN"
gene complement(170066..171697)
/gene="Izh3"
/locus_tag="R008_L10511"
mRNA complement(170066..171697)
/gene="Izh3"
/locus_tag="R008_L10511"
/product="Izh3p"
CDS complement(170066..171697)
/gene="Izh3"
/locus_tag="R008_L10511"
/note="Similar to YLR023C 'Membrane protein involved in
zinc ion homeostasis, member of the four-protein IZH
family, expression induced by zinc deficiency; deletion
reduces sensitivity to elevated zinc and shortens lag
phase, overexpression reduces Zap1p activity'"
/codon_start=1
/product="Izh3p"
/protein_id="EWG84302.1"
/translation="MMDSSSKSLTQYIPSPMGSLSRLKQKGVDNFQKVKKSGKSIYNY
NYSKFVPHPFSTIDESVKHSESGRYDDLEIIRPTKEKEVTSSVYKRNSGKSLNTESQF
SLGDSDAATLVNSVATFKLNNASTSTSLVSSSSTVCSQAKSSLRSPTSRLNDTKIKEE
NNYISSVKDYCGPMRKSMVKTEILIEEPLNPTTDIKSFINSYNHGKAYSLGETQHLHY
YQLPFPWRENRYIIHGYRFYNTHSKSLLSIFNWYGWHNETSNIWSHLLGAIYIIYLAI
YDFPQSEVWRNSQVPPQARWIVFMFLAAALKCMLSSVFWHTFNGTSFLKLRSKFACVD
YSGITILITASILTTEFVTMYSCYWAMCTYMSISLALGVFGVFMNWSPRFDRPEARPL
RIRFFILLATMGVLSFLHLIFLTDLHYAATLFSPVTYKSVVWYLVGVVFYGSFIPERF
RSDVQVDKTIPTNYELSTDLEIITKQREIHFREVPTAHSKCSSCPSHAKSFKSLWWVD
YFGCSHTFWHFFVVLGVIGHYRAILDMFAKRWILS"
gene complement(172230..177847)
/gene="Ubr2"
/locus_tag="R008_L10516"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 179018..179740
/gene="Snf7"
/locus_tag="R008_L10526"
mRNA 179018..179740
/gene="Snf7"
/locus_tag="R008_L10526"
/product="Snf7p"
CDS 179018..179740
/gene="Snf7"
/locus_tag="R008_L10526"
/note="Similar to YLR025W 'One of four subunits of the
endosomal sorting complex required for transport III
(ESCRT-III); involved in the sorting of transmembrane
proteins into the multivesicular body (MVB) pathway;
recruited from the cytoplasm to endosomal membranes'"
/codon_start=1
/product="Snf7p"
/protein_id="EWG84303.1"
/translation="MWSSLFGWTSSNAKNKESPTKAIVRLREHINLLSKKQSHLRTQI
TNQENEARIFLTKGNKVMAKNALKKKKTIEQLLSKVEGTMESMEQQLFSIESANLNLE
TMRAMQEGAKAMKTIHSGLDIDKVDETMDEIREQVELGDEISDAISRPLITGANEVDE
DELDEELDMLAQENANQETSKIVNNNVNAAPISENKVSLPSVPSNKIKQSENSVKDGE
EEEDEEDEDEKALRELQAEMGL"
gene complement(180014..181036)
/gene="Sed5"
/locus_tag="R008_L10531"
mRNA complement(180014..181036)
/gene="Sed5"
/locus_tag="R008_L10531"
/product="Sed5p"
CDS complement(180014..181036)
/gene="Sed5"
/locus_tag="R008_L10531"
/note="Similar to YLR026C cis-Golgi t-SNARE syntaxin
required for vesicular transport between the ER and the
Golgi complex, binds at least 9 SNARE proteins"
/codon_start=1
/product="Sed5p"
/protein_id="EWG84304.1"
/translation="MNIKDRTSEFQQSVLSYKKRNKNFREQQRERLQEKESENFANNT
TGNGKSVSEFQKKASGIAHEISSTAQLLSKLAVLAKRKPMFNDNPVEIAELSFLIKRK
IYAIEQSLVQLSQLKKTDVNGNTSNQSSKQPSAVQHSKNVVNLLNTQMKNISGSFKDV
LEERQRLEMANKDRWQKLTTDTGHAPADDQTQSNHAADLTTYNNSNPFMTSLLDESSE
KNNNSSNQGELSFPQNDSQLMLMEEGQLSNNVYLQERNRAVETIESTIQEVGNLFQQL
ASMVQEQGEVIQRIDANVDDIDLNISGAQRELLKYFDRIKSNRWLAAKVFFIIFVFFV
IWVLVN"
gene complement(181388..182644)
/gene="Aat2"
/locus_tag="R008_L10536"
mRNA complement(181388..182644)
/gene="Aat2"
/locus_tag="R008_L10536"
/product="Aat2p"
CDS complement(181388..182644)
/gene="Aat2"
/locus_tag="R008_L10536"
/note="Similar to YLR027C 'Cytosolic aspartate
aminotransferase, involved in nitrogen metabolism;
localizes to peroxisomes in oleate-grown cells'"
/codon_start=1
/product="Aat2p"
/protein_id="EWG84305.1"
/translation="MSATLFNNIELLPPDALFGIKQRYGQDQRATKVDLGIGAYRDDN
GKPWVLPSVKAAEKLIHNDSSYNHEYLGITGLPSLTSNAAKIIFGTQSDAFQEDRVIS
VQSLSGTGALHISAKFFSKFFPDKLVYLSKPTWANHMAIFENQGLKTATYPYWANETK
SLDLNGFLNAIQKAPEGSIFVLHSCAHNPTGLDPTSEQWVQIVDAIASKNHIALFDTA
YQGFATGDLDKDAYAVRLGVEKLSTVSPVFVCQSFAKNAGMYGERVGCFHLALTKQAQ
NKTIKPAVTSQLAKIIRSEVSNPPAYGAKIVAKLLETPELTEQWHKDMVTMSSRITKM
RHALRDHLVKLGTPGNWDHIVNQCGMFSFTGLTPQMVKRLEETHAVYLVASGRASIAG
LNQGNVEYVAKAIDEVVRFYATEAKL"
gene 183343..183948
/locus_tag="R008_L10541"
ncRNA 183343..183948
/ncRNA_class="snoRNA"
/locus_tag="R008_L10541"
/product="SNR30"
gene complement(184099..185874)
/gene="Ade16"
/locus_tag="R008_L10546"
mRNA complement(184099..185874)
/gene="Ade16"
/locus_tag="R008_L10546"
/product="Ade16p"
CDS complement(184099..185874)
/gene="Ade16"
/locus_tag="R008_L10546"
/note="Similar to YLR028C 'Enzyme of 'de novo' purine
biosynthesis containing both
5-aminoimidazole-4-carboxamide ribonucleotide
transformylase and inosine monophosphate cyclohydrolase
activities, isozyme of Ade17p; ade16 ade17 mutants require
adenine and histidine'"
/codon_start=1
/product="Ade16p"
/protein_id="EWG84306.1"
/translation="MGKYTKTAILSVYDKTGLLDLAKGLVENNVRILASGGTANMVRE
AGFPVDDVSSITHAPEMLGGRVKTLHPAVHAGILARNLEGDEKDLKEQHIDKVDFVVC
NLYPFKETVAKIGVTVQEAVEEIDIGGVTLLRAAAKNHSRVTILSDPNDYSIFLQDLS
KDGEISQDLRNRFALKAFEHTADYDAAISDFFRKQYSEGKAQLPLRYGCNPHQRPAQA
YITQQEELPFKVLCGTPGYINLLDALNSWPLVKELSASLNLPAAASFKHVSPAGAAVG
LPLSDVEKQVYFVNDMEDLSPLACAYARARGADRMSSFGDFIALSNIVDVATAKIISK
EVSDGVIAPGYEPEALNILSKKKNGKYCILQIDPNYVPGQMESREVFGVTLQQKRNDA
IINQSTFKEIVSKNKALTEQAVIDLTVATLVLKYTQSNSVCYAKNGMVVGLGAGQQSR
IHCTRLAGDKTDNWWLRQHPKVLNMKWAKGIKRADKSNAIDLFVTGQRIEGPEKVDYE
SKFEEVPEPFTKEERLEWLSKLNNVSLSSDAFFPFPDNVYRAVQSGVKFITAPSGSVM
DKVVFQAADSFDIVYVENPIRLFHH"
gene complement(186554..187168)
/gene="Rpl15a"
/locus_tag="R008_L10556"
mRNA complement(186554..187168)
/gene="Rpl15a"
/locus_tag="R008_L10556"
/product="Rpl15ap"
CDS complement(186554..187168)
/gene="Rpl15a"
/locus_tag="R008_L10556"
/note="Similar to YLR029C 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl15Bp and
has similarity to rat L15 ribosomal protein; binds to 5.8
S rRNA'"
/codon_start=1
/product="Rpl15ap"
/protein_id="EWG84307.1"
/translation="MGAYKYLEELQRKKQSDVLRFLQRVRVWEYRQKNVIHRAARPTR
PDKARRLGYKAKQGFVIYRVRVRRGNRKRPVPKGATYGKPTNQGVNELKYQRSLRATA
EERVGRRAANLRVLNSYWVNQDSTYKYFEVILVDPQHKAIRRDARYNWICDPVHKHRE
ARGLTATGKKSRGINKGHKFNNTKAGRRKTWKRQNTLSLWRYRK"
gene 187867..189363
/locus_tag="R008_L10566"
mRNA 187867..189363
/locus_tag="R008_L10566"
/product="hypothetical protein"
CDS 187867..189363
/locus_tag="R008_L10566"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84308.1"
/translation="MEGEQIMEYVQETPIIPKRIIHYSIPKQMITKPAPHVEMTLLAN
TFRDMDLPQHPVIHDCWQNKEHSTQRYSGNVAQQRLSFEEHPNEECQNSVGLIKRVST
FFKKRPLSRKNSIKSIGDVKSEARNRGEGLLGEVDNLNEQNVRENLTSEHEKSPEGDS
KRYGLFSFEETPPIQVLEQGNINSELSSFKNTSLAENKRSSDSFVSLKPGEDEHSPLE
ISTCGNLTEREDLQSGEERFDSAAQNIKVASMKEKKKILQREQNRLITEIIRLENILN
KHRKAEAKNNINKLEKKLSETDGFTSVNTSVPVTPIMPVLNTRTSYPNIDFHGTKVSD
VLDAFEFEKHDDPLRDKWNTLQFLEKSFESKFESASELIQGGELAAIKERNFQLAKLN
NLCFRVRESIKRRQDLEKKLRTLSQDTDNELLFLMLENERRKKSSVIIEFLSEIIREK
SKRLTAEEQGFVNQNEVKPLILDLSARINRLNSILETKNTCIRRLSNQ"
gene 189569..193078
/gene="Rad5"
/locus_tag="R008_L10576"
mRNA 189569..193078
/gene="Rad5"
/locus_tag="R008_L10576"
/product="Rad5p"
CDS 189569..193078
/gene="Rad5"
/locus_tag="R008_L10576"
/note="Similar to YLR032W 'DNA helicase proposed to
promote replication fork regression during postreplication
repair by template switching; RING finger containing
ubiquitin ligase; stimulates the synthesis of free and
PCNA-bound polyubiquitin chains by Ubc13p-Mms2p'"
/codon_start=1
/product="Rad5p"
/protein_id="EWG84309.1"
/translation="MSHIEQEERKRFFNDDLDTSETSLNFKSENKESFLFANSHNDDD
DDVVVSVSDTTEGEGDRSIVPVRREIEEEGQNQFITELLRIIPEMPKDLVMELNEKFG
SQEEGLSLALSHYFDHNSGTSISKIPSSPNQLNTLSDTSNSTLSPSSFHPKRRRIYGF
RNQTRLEDKVTWKRFIGALQVTGMATRPTVRPLKYGSQMKLKRSSEEISATKVYDSRG
RKKASMASLVRIFDIQYDREIGRVSEDIAQILYPLLSSHEISFEVTLIFCDNKRLSIG
DSFILQLDCFLTSLIFEERNDGESLMKRRRTEGGNKREKDNGNFGRTLTETDEELESR
SKRLALLKLFDKLRLKPILDEQKALEKHKIELNSDPEIIDLDNDEICSNQVTEVHNNL
RDTQHEEETMNLNQLKTFYKAAQSSESLKSLPETEPSRDVFKLELRNYQKQGLTWMLR
REQEFAKAASDGEASETGANMINPLWKQFKWPNDMSWAAQNLQQDHVNVEDGIFFYAN
LHSGEFSLAKPILKTMIKGGILSDEMGLGKTVAAYSLVLSCPHDSDVVDKKLFDIENT
AVSDNLPSTWQDNKKPYASKTTLIVVPMSLLTQWSNEFTKANNSPDMYHEVYYGGNVS
SLKTLLTKTKTPPTVVLTTYGIVQNEWTKHSKGRMTDEDVNISSGLFSVNFYRIIIDE
GHNIRNRTTVTSKAVMALQGKCKWVLTGTPIINRLDDLYSLVKFLELDPWRQINYWKT
FVSTPFESKNYKQAFDVVNAILEPVLLRRTKQMKDKDGKPLVELPPKEVVIKRLPFSK
SQDLLYKFLLDKAEVSVKSGIARGDLLKKYSTILVHILRLRQVCCHPGLIGSQDENDE
DLSKNNKLVTEQTVELDSLMRVVSERFDNSFSKEELDAMIQRLKVKYPDNKSFQSLEC
SICTTEPMDLDKALFTECGHSFCEKCLFEYIEFQNSKNLGLKCPNCRNQIDACRLLAL
VQTNSNSKNLEFKPYSPASKSSKITALLKELQLLQDSSAGEQVVIFSQFSTYLDILEK
ELTHTFSKDVAKIYKFDGRLSLKERTSVLADFAVKDYSRQKILLLSLKAGGVGLNLTC
ASHAYMMDPWWSPSMEDQAIDRLHRIGQTNSVKVMRFIIQDSIEEKMLRIQEKKRTIG
EAMDTDEDERRKRRIEEIQMLFE"
gene 193339..194847
/gene="Rsc58"
/locus_tag="R008_L10581"
mRNA 193339..194847
/gene="Rsc58"
/locus_tag="R008_L10581"
/product="Rsc58p"
CDS 193339..194847
/gene="Rsc58"
/locus_tag="R008_L10581"
/note="Similar to YLR033W 'Component of the RSC chromatin
remodeling complex; RSC functions in transcriptional
regulation and elongation, chromosome stability, and
establishing sister chromatid cohesion; involved in
telomere maintenance'"
/codon_start=1
/product="Rsc58p"
/protein_id="EWG84310.1"
/translation="MTESVGGNKLVDLLVNVQSILNAASVKCHVVDESFPAKFFEKNP
DKIYESYCKFIKNRSNSEGLIRNEDKLVLTTINKRFENGEYEPIQGGFYKLYHDIKLV
CTILIHFYPQGTRNYQLVDKFYKFSSELLLRECCRIGIALTQTNNIKSRSGKLLSGNE
MDEYDDDDATELDKIISYDFIKISMNYTVPISQTYQIRTKDMDLFSSIISKSNLDKRP
HELPNTNFKINNVLPQTDIENEAPRLGFVGANTSNIPDPTLPPTEMMTRFLHPNWYAL
PTTVWLKYGNYNSWAPSFNENGTVVDSTTRGLIWLERIGYMDLYEKNEKKVKQEELLN
TNEEGINRKQNDENNKNVDGKSNGVQDDGGDNDNDATIASANSESTENKEQFIIKLQN
LYNWTPSNYIGDDEIENFRNGTPDKLVSDSLLKLKRLRKERILNKVLKPTTEERELYF
KVKRILKEVILAKKVSKFPINNVRAFPVLQTNYNGSIPVVRAQPGRKRKHKK"
gene complement(195090..196512)
/gene="Smf3"
/locus_tag="R008_L10586"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 196423..196437
/estimated_length=15
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(196948..199035)
/gene="Mlh2"
/locus_tag="R008_L10591"
mRNA complement(196948..199035)
/gene="Mlh2"
/locus_tag="R008_L10591"
/product="Mlh2p"
CDS complement(196948..199035)
/gene="Mlh2"
/locus_tag="R008_L10591"
/note="Similar to YLR035C 'Protein involved in the
mismatch repair and involved in meiotic recombination;
forms a complex with Mlh1p'"
/codon_start=1
/product="Mlh2p"
/protein_id="EWG84311.1"
/translation="MTIHQLSPESQWKIVSSSFIYGPVAAVRELLDNSIDSGAKKVFI
DVDSTTGGCEYISVKDDGSGVDIIDRPSMCLEYTTSKMSSLGDISILTTLGFRGEALF
LLSNLCNQKGSMQVETKTADDVIGEKWLVDSKGGITNGKRYKVSCPVGTTVTLRKLLG
GLRARYLEISSRPRKTFDELIYLINHYSLIHRNIRFYFSLVSLQKNGAIERKQMQETL
DPKISRARSLSLLARLKKPVPLNFIVEENFVIDEKINLDLILPRMVPESDVINIKRRF
KFLSVNERALSLNLETGKTISKLLSSIYRDFSLLDPMVWFINLNCDTKLLDVNIEPEK
NDVMIKSFEVIFKKIENKLKLLLEERIGIETNMLGDKHVQPSINEKTSPALVIPTPDA
ENEISKGCGAVSGKDKTDIPQKNSDLIVPTFYDEANLENTTIVAATPSPTKFSEDKAL
DEQTQLTISSYRSSSSGSMASEDSTNWRHNFQQELSENSEVAGAGSSTLPSSLTYNYI
ETIPENEDLELSKDASISNPFMITKIRNVNKKLSENLLEAKRTSCGDANKRGMPNERQ
LSNTEKKELIKLQQTYGKRNNTVDMTIPRNSKKKVTDNYIKKASCMHKTRPKLMHFSE
YTNNYVYTLKNEKIVKHDSDNFAKETLWLRSRDDATSPSSSLLQALRAHVKKPGHIEA
TTNEWCLFTPDSP"
gene complement(199462..199534)
/locus_tag="R008_L10596"
tRNA complement(199462..199534)
/locus_tag="R008_L10596"
/product="tRNA-Ala"
/note="tRNA-Ala (Ala2);
TA(UGC)L"
gene complement(200169..200780)
/locus_tag="R008_L10601"
mRNA complement(200169..200780)
/locus_tag="R008_L10601"
/product="hypothetical protein"
CDS complement(200169..200780)
/locus_tag="R008_L10601"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84312.1"
/translation="MKEAELSATESQDEIPKSNSLLIIEKLTKAVCSLYFINCFMVPS
VDNLIEKYPKAIIIKIIDMILGAVTISLVIIVFFLYRKNGHFKNENKTKPKRCSKVVC
PSCAARKKYPKWFQLKYLLLVSMTAFSFYFCTKIRFFFKTDQTINLHRLSQLFRLQLG
WICTTALLFYFYDALILHSGFIEGYRCVNGKGAMSEGKTGQLN"
gene complement(201333..201707)
/gene="Dan2"
/locus_tag="R008_L10611"
mRNA complement(201333..201707)
/gene="Dan2"
/locus_tag="R008_L10611"
/product="Dan2p"
CDS complement(201333..201707)
/gene="Dan2"
/locus_tag="R008_L10611"
/note="Similar to YLR037C 'Cell wall mannoprotein with
similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of
the seripauperin multigene family encoded mainly in
subtelomeric regions; expressed under anaerobic
conditions, completely repressed during aerobic growth'"
/codon_start=1
/product="Dan2p"
/protein_id="EWG84313.1"
/translation="MVKLTSIVAGVAAIAAGVAAAPATTTLSPSDERVNLVELGVYVS
DIRAHLAEYYMFQAAHPTETYPVEIAEAVFNYGDFTTMLTGIPADQVTRVITGVPWYS
TRLRPAISSALSKDGIYTAVPN"
gene complement(203569..203820)
/gene="Cox12"
/locus_tag="R008_L10626"
mRNA complement(203569..203820)
/gene="Cox12"
/locus_tag="R008_L10626"
/product="Cox12p"
CDS complement(203569..203820)
/gene="Cox12"
/locus_tag="R008_L10626"
/note="Similar to YLR038C 'Subunit VIb of cytochrome c
oxidase, which is the terminal member of the mitochondrial
inner membrane electron transport chain; required for
assembly of cytochrome c oxidase but not required for
activity after assembly; phosphorylated'"
/codon_start=1
/product="Cox12p"
/protein_id="EWG84314.1"
/translation="MADQENSPLHTVGFDARFPQQNQTKHCWQSYVDYHKCVNMKGED
FAPCKVFWKTYNALCPLDWIEKWDDQREKGIFAGDINSD"
gene complement(204074..207243)
/gene="Ric1"
/locus_tag="R008_L10631"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(207555..208229)
/locus_tag="R008_L10636"
mRNA complement(207555..208229)
/locus_tag="R008_L10636"
/product="hypothetical protein"
CDS complement(207555..208229)
/locus_tag="R008_L10636"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84315.1"
/translation="MIFAPSFSLIKNILLVSFLISHSFAAKTLTSSSNDDTLARSAAA
DADMAFFMEFLNDFDTAFPQYTSYMMQNHLTLPQPVADYYYHMVDLASTADLQSDIAQ
SFPFTQFQTFITAFPWYTSLLNKASATTIYLPQHFITGETEATMTNSSYASQKNSVSN
SVPFSTANAGQSMISMANEENSTTALISASNSSSTSRTSQSQNGAHAKSLYFPMALFG
IFAVAL"
gene complement(208613..209098)
/locus_tag="R008_L10651"
mRNA complement(208613..209098)
/locus_tag="R008_L10651"
/product="hypothetical protein"
CDS complement(208613..209098)
/locus_tag="R008_L10651"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84316.1"
/translation="MKISQFGSLAFAPIVLLQLFIVQAQLLTDSNAQDLNTALGQKVQ
YTFLDTGNSNDQLLHLPSTTSSSIITGSLAAANFTGSSSSSSIPKVTSSVITSINYQS
SNSTVVTQFTPLPSSSRNETKSSQTTNTISSSTSTGGVGSVKPCLYFVLMLETIAYLF
S"
gene complement(210349..210660)
/gene="Trx1"
/locus_tag="R008_L10661"
mRNA complement(210349..210660)
/gene="Trx1"
/locus_tag="R008_L10661"
/product="Trx1p"
CDS complement(210349..210660)
/gene="Trx1"
/locus_tag="R008_L10661"
/note="Similar to YLR043C Cytoplasmic thioredoxin
isoenzyme of the thioredoxin system which protects cells
against oxidative and reductive stress, forms LMA1 complex
with Pbi2p, acts as a cofactor for Tsa1p, required for
ER-Golgi transport and vacuole inheritance"
/codon_start=1
/product="Trx1p"
/protein_id="EWG84317.1"
/translation="MVTQFKTASEFDSAIAQDKLVVVDFYATWCGPCKMIAPMIEKFS
EQYPQADFYKLDVDELGDVAQKNEVSAMPTLLLFKNGKEVAKVVGANPAAIKQAIAAN
A"
gene complement(211036..212727)
/gene="Pdc1"
/locus_tag="R008_L10666"
mRNA complement(211036..212727)
/gene="Pdc1"
/locus_tag="R008_L10666"
/product="Pdc1p"
CDS complement(211036..212727)
/gene="Pdc1"
/locus_tag="R008_L10666"
/note="Similar to YLR044C 'Major of three pyruvate
decarboxylase isozymes, key enzyme in alcoholic
fermentation, decarboxylates pyruvate to acetaldehyde;
subject to glucose-, ethanol-, and autoregulation;
involved in amino acid catabolism'"
/codon_start=1
/product="Pdc1p"
/protein_id="EWG84318.1"
/translation="MSEITLGKYLFERLKQVNVNTVFGLPGDFNLSLLDKIYEVEGMR
WAGNANELNAAYAADGYARIKGMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVP
SISAQAKQLLLHHTLGNGDFTVFHRMSANISETTAMITDIATAPAEIDRCIRTTYVTQ
RPVYLGLPANLVDLNVPAKLLQTPIDMSLKPNDAESEKEVIDTILALVKDAKNPVILA
DACCSRHDVKAETKKLIDLTQFPAFVTPMGKGSIDEQHPRYGGVYVGTLSKPEVKEAV
ESADLILSVGALLSDFNTGSFSYSYKTKNIVEFHSDHMKIRNATFPGVQMKFVLQKLL
TTIADAAKGYKPVAVPARTPANAAVPASTPLKQEWMWNQLGNFLQEGDVVIAETGTSA
FGINQTTFPNNTYGISQVLWGSIGFTTGATLGAAFAAEEIDPKKRVILFIGDGSLQLT
VQEISTMIRWGLKPYLFVLNNDGYTIEKLIHGPKAQYNEIQGWDHLSLLPTFGAKDYE
THRVATTGEWDKLTQDKSFNDNSKIRMIEIMLPVFDAPQNLVEQAKLTAATNAKQ"
gene complement(213683..216349)
/gene="Stu2"
/locus_tag="R008_L10676"
mRNA complement(213683..216349)
/gene="Stu2"
/locus_tag="R008_L10676"
/product="Stu2p"
CDS complement(213683..216349)
/gene="Stu2"
/locus_tag="R008_L10676"
/note="Similar to YLR045C 'Microtubule-associated protein
(MAP) of the XMAP215/Dis1 family; regulates microtubule
dynamics during spindle orientation and metaphase
chromosome alignment; interacts with spindle pole body
component Spc72p'"
/codon_start=1
/product="Stu2p"
/protein_id="EWG84319.1"
/translation="MSGEEEVDYTTLPLEERLTYKLWKARLEAYKELNQLFRNSVGDI
SRDDNIQIYWRDPTLFAQYITDSNVVAQEQAIVALNSLIDAFASSSLKNAHNITLIST
WTPLLVEKGLTSSRATTKTQSMSCILSLCGLDTSITQSVELVIPFFEKKLPKLIAAAV
NCVYELMAAFGLTNVNVQTFLPELLKHVPQLAGHGDRNVRSQTMNLIVEIYKVTGNNS
DLLEEILFKKLKPIQVKDLHKLFAKVGDEPSSSKMLFEWEKRELEKKRSQEEEARKRK
SILSNDEGEYQIDKDGDTLMGMETDMPPSKQQSGVQIDTFSMLPEETILDKLPKDFQE
RITSSKWKDRVEALEEFWDSVLSQTKKLKSSSQNYSNLLGIYGHIIQKDANIQAVALA
AQSVELICDKLKTPGFSKDYVSLVFTPLLDRTKEKKPSVIEAIRKALLTICKYYDPLA
SSGRNEDMLKDILEHMKHKTPQIRMECTQLFNASMKEEKDGYSTLQRYLKDEVVPIVI
QIVNDTQPAIRTIGFESFAILIKIFGMNTFVKTLEHLDNLKRKKIEETVKTLPNFSIA
SGSTHSTIETNKQTGPMENKFLLKKSSVLPSKRVASSPLRNDNKSKVNPIGSVASASK
PSMVAANNKSRILLTSKSLATPKNVVANSTDKNEKLIEEYKYRLQKLQNDEMIWTKER
QSLLEKMNNTENYKIEMIKENEMLREQLKEAQSKLNEKNIQLRSKEIDVNKLSDRVLS
LENELRNMEIELDRNKKRNDTNLQSMGTISSYSIPSSTVSSNYGVKSLSSVLPFKEEE
DVRRKEDVNYERRSSESIGDLPHRVNSLNIRPYRKNGTGVSSVSDDLDIDFNDSFASE
ESYKRAAAVTSTLKARIEKMKAKSRREGTTRT"
gene complement(216647..217459)
/gene="Rla1"
/locus_tag="R008_L10681"
mRNA complement(216647..217459)
/gene="Rla1"
/locus_tag="R008_L10681"
/product="Rla1p"
CDS complement(216647..217459)
/gene="Rla1"
/locus_tag="R008_L10681"
/note="Similar to YLR046C 'Putative membrane protein;
member of the fungal lipid-translocating exporter (LTE)
family of proteins; transcription is activated by
paralogous transcription factors Yrm1p and Yrr1p along
with genes involved in multidrug resistance'"
/codon_start=1
/product="Rla1p"
/protein_id="EWG84320.1"
/translation="MATHTSKRRIHRWENNELLEENSTIIYFPARGLMWTHFPFVLGI
CLEFVGYVLKIVFINSPSISTFIAQSVLLLIAPSLYALSIFMLFSKMARLILMEAYML
IPAKFSTVSFVVADMIGRVLQAVGGGLLSSWNSRNTGRILIIVGLFIQIFCYTFLTFS
QLFLHYKMKAAPSKIVRDSNEWFQYNFMLLAGILLVNGRTIVRVVQFLMGLQSYIGQH
EWCLYVFDTVLMFLLPLIFLATFRARNLSKLQDKSVNIQLNKLLDKESVSED"
gene complement(217993..220053)
/gene="Fre8"
/locus_tag="R008_L10686"
mRNA complement(217993..220053)
/gene="Fre8"
/locus_tag="R008_L10686"
/product="Fre8p"
CDS complement(217993..220053)
/gene="Fre8"
/locus_tag="R008_L10686"
/note="Similar to YLR047C 'Protein with sequence
similarity to iron/copper reductases, involved in iron
homeostasis; deletion mutant has iron
deficiency/accumulation growth defects; expression
increased in the absence of copper-responsive
transcription factor Mac1p'"
/codon_start=1
/product="Fre8p"
/protein_id="EWG84321.1"
/translation="MNLKSIVSWFKEHLPSFDVDVDKHFRTLRVRKYSQICLLISFII
ICVIIPLMNYLLLTDKFFKICHHLKHHVFNRRSWVHKTRMYHKQSLQLCLICFVFTSF
FVIQGANGDLLEITKRMGRISVALMPPLLFLTLRPSPLPHTLYLALLPLHKWISRIVV
LESILHTWFYLYYMYINDTLYVKMRKLPNIYGVIALGLFLLIAITSVRYARRWSYRVF
YYVHYVSTWLILVFLHYHARPGISYYTTLNVLILTGQIVYRLYITNVTRVTIVPISSS
LSLLEFPLTDLPKKPILPGGHLRINIYHRNFLRRFFSHLIPFQHPFTIASIPSDNLVR
LIIRNGHFPLRTNEKYYITGAFEPELSFISKPTVPFNITTKSSKNPFRNNSSALINSP
LNFLIKAQRVFMCVGGSGISFGLPLLRILNFNGVNVRLLWVSRDYKDLEVLNHFKNNF
EGMEIYISGTEGNEQDIEIDYIDYHDCAADINDEVRSISSSGRVSELGDNSMLSDGNP
QPTEPNENTALLSKKSTLRNHHPPKTNDIPDINADDEIDFTYAFSRSKSRKNTAQGTL
TTHSSFNGSSVFRQPKIIEPPAQDPCLEAAPKKIRIPAGVKVFFGRPTLGDKDYEWCL
QTECDAETDSIQCCRWANQGRDHAEYLSQVWVLAAGPRGLIESTKRWATDGGLHFHGE
SFAL"
gene 220875..221992
/gene="Rps0b"
/locus_tag="R008_L10691"
mRNA join(220875..220964,221324..221992)
/gene="Rps0b"
/locus_tag="R008_L10691"
/product="Rps0bp"
CDS join(220875..220964,221324..221992)
/gene="Rps0b"
/locus_tag="R008_L10691"
/note="Similar to YLR048W 'Protein component of the small
(40S) ribosomal subunit, nearly identical to Rps0Ap;
required for maturation of 18S rRNA along with Rps0Ap;
deletion of either RPS0 gene reduces growth rate, deletion
of both genes is lethal'"
/codon_start=1
/product="Rps0bp"
/protein_id="EWG84322.1"
/translation="MSLPATFDLTPEDAQLLLAANTHLGARNVQVHQEPYVFNARPDG
VHVINVGKTWEKLVLAARIIAAIPNPEDVVAISSRTYGQRAVLKFAAHTGATPIAGRF
TPGSFTNYITRSFKEPRLVIVTDPRLDAQAIKEASYVNIPVIALTDLDSPSEFVDVAI
PCNNRGKHSIGLIWYLLAREVLRLRGALVDRTQPWSIMPDLYFYRNPEEVEQVAEEAA
AAEEGEEEEVKEEVTEGQAEATEWAEENADNVEW"
gene complement(222541..223827)
/locus_tag="R008_L10696"
mRNA complement(222541..223827)
/locus_tag="R008_L10696"
/product="hypothetical protein"
CDS complement(222541..223827)
/locus_tag="R008_L10696"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84323.1"
/translation="MVNNIMHEYVPPSQRLFHSRHRITRNDLKEEALHSGTTDWTTIL
DTTIDKDTNLISYAVPIIDNFAIPRANSQGSPVAPSPNHRSTMYSSSSSSASSVFSDG
LFTPNNNRNSSGSSSLVIRPQKNLSVDSLIQENKRKINSEKESLSLIANNNDETLCTH
TDPSIQNLIKSETKRNILNLKFQNRNLFRRELKLEKFWSNLRSCHTSGDETDLLLVIS
KHNLYWFGIPNDFRLPIYKRCLYHYSELDEAGFFSQYANNSLYLAIRKCCNNEEQETL
SRSIFINLTKNVTWLNSRFDDNKDNKNSYTVTEGKFYQDFPNLYYHLKDKLKLNVIMD
FIKPVIRNFMTNALNKHKLDGIGLELLDILIVTTYYGPNKINAFLMDTFILNLLKQCH
YKFFVSNISELVIQISKIDCDLVILLEDLRSRIDLD"
gene complement(224239..224724)
/locus_tag="R008_L10706"
mRNA complement(224239..224724)
/locus_tag="R008_L10706"
/product="hypothetical protein"
CDS complement(224239..224724)
/locus_tag="R008_L10706"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84324.1"
/translation="MKLGHREQQFYLWYFIVHIPITIFIDSSVVIPAKWQLGIAQKVV
SDHIAKQHDFLLSEKPEWLYWFVVLELVLQLPLFVYFVNEFWNSSELQVNKNSRLKKW
LRIYGWNASLTTLICIVVIFKRGYIPYDVLKTSLSMTQKCQLASVYLPTFLIPLRLCF
V"
gene complement(224975..225628)
/gene="Fcf2"
/locus_tag="R008_L10711"
mRNA complement(224975..225628)
/gene="Fcf2"
/locus_tag="R008_L10711"
/product="Fcf2p"
CDS complement(224975..225628)
/gene="Fcf2"
/locus_tag="R008_L10711"
/note="Similar to YLR051C 'Essential nucleolar protein
involved in the early steps of 35S rRNA processing;
interacts with Faf1p; member of a transcriptionally
co-regulated set of genes called the RRB regulon'"
/codon_start=1
/product="Fcf2p"
/protein_id="EWG84325.1"
/translation="MDQSVEDLFGALRDASASLEAKNSAKEQVSLQQEDVLQIGNNDD
EVEIESKFQEIETNLKKLPKLETGFDALANKKKKKNVLPSVETEDKRKPYKSDKNDND
WFTLPKPDDNMRREVQRDLLLIKHRAALDPKRHYKKQRWEVPERFAIGTIIEDKSEFY
SSRMNRKERKSTILETLMGDEASNKYFKRKYNEIQEKSTSGRKAHYKKMKEMRKKRR"
gene 225852..226604
/gene="Ies3"
/locus_tag="R008_L10716"
mRNA 225852..226604
/gene="Ies3"
/locus_tag="R008_L10716"
/product="Ies3p"
CDS 225852..226604
/gene="Ies3"
/locus_tag="R008_L10716"
/note="Similar to YLR052W Subunit of the INO80 chromatin
remodeling complex"
/codon_start=1
/product="Ies3p"
/protein_id="EWG84326.1"
/translation="MKFEDLLATNKQVQFAHAATQHYKSVKTPDFLEKDPHHKKFHNA
DGLNQQGSSTPSTATDANAAPTASTHTNTTTFKRHIVAVDDISKMNYEMIKNSPGNVI
TNANQDEIDISTLKTRLYKDNLYAMNDNFLQAVNDQIVTLNAAEQDQETEDPDLSDDE
KIDILTKIQENLLEEYQKLSQKERKWFILKELLLDANVELDLFSNRGRKASHPIAFGA
VAIPTNVNANSLAFNRTKRRKINKNRLLENIL"
gene complement(226751..227077)
/locus_tag="R008_L10726"
mRNA complement(226751..227077)
/locus_tag="R008_L10726"
/product="hypothetical protein"
CDS complement(226751..227077)
/locus_tag="R008_L10726"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84327.1"
/translation="MDMLHNKCSDAIKSTSNSNLSNEVDKQKLQYDDLGNTAFSELFE
MESQDNNDNIEDFLFFNINLTQEVEFENQRQYEHTKKTKKHNPFYVPSEVVREMVKKH
ALNGRI"
gene complement(227364..229625)
/gene="Osw2"
/locus_tag="R008_L10731"
mRNA complement(join(227364..229507,229595..229625))
/gene="Osw2"
/locus_tag="R008_L10731"
/product="Osw2p"
CDS complement(join(227364..229507,229595..229625))
/gene="Osw2"
/locus_tag="R008_L10731"
/note="Similar to YLR054C Protein of unknown function
proposed to be involved in the assembly of the spore wall"
/codon_start=1
/product="Osw2p"
/protein_id="EWG84328.1"
/translation="MEENQLTCLIVGETPAAQFLGWRLSLSNSFIILVSQYVSSDELV
AWKSTKLGANFYTPNIFTKDIKELHHKLKHNGANTYSIDIVLVSAISLQSFETTCRLL
SDYTNDNTTVLISTDFGCELEPIAISYFGGKCKCVISISCEVECRQLSLGSYALVNDD
QCVITLGLTYCDANFEANPTILENTKAASLELQGIKGSNVRRFLLGLTVAKWMKSKLI
LDPKQMALKMWELLIPKISLNILSIIYEQFDYEKMLENKSTEIIFKDLVKELLGICFA
QCGSKIARFLLVKSQGEEEINFGKIVEYCKGKKLQLINSTANEHPEYLSLPFEPYCFY
HRFEYPAQILLHQPIILAKKYGVSCSNLNFLYGFYTRLLTLSGLSINGGRCEHALSML
DSRIGGGINVASGINSGQDFTDGDNKDQDKGKNRVDKNVKEGSFISLTQRFTTTSPLG
VNDPALPADLEKLYLGAEYISNCDANTSGGQKRVKSPTKADTGYDDKHLPDDTIHTFE
DEYLADEDDFSCLGIDKRSSTKPTKPLEKFGVVAVPHFIRRFSIKRSSKDKSNDMKRP
YTTSSLELQLRSNHFMFAKEYQDLHRQLYYEVKPRTQSELDARRRNYAELESQMWKIK
HRFNIHRGALPRPRTNPYELLLDHIDVLNRGNTGDILRFTTSRYGGVDTYDSILRDQS
TIMELLDKRCAYSPPVLFDNEAKERDHDRDCHQDYNHDHRSH"
gene complement(229916..231718)
/gene="Spt8"
/locus_tag="R008_L10741"
mRNA complement(229916..231718)
/gene="Spt8"
/locus_tag="R008_L10741"
/product="Spt8p"
CDS complement(229916..231718)
/gene="Spt8"
/locus_tag="R008_L10741"
/note="Similar to YLR055C Subunit of the SAGA
transcriptional regulatory complex but not present in
SAGA-like complex SLIK/SALSA, required for SAGA-mediated
inhibition at some promoters"
/codon_start=1
/product="Spt8p"
/protein_id="EWG84329.1"
/translation="MDEVDDILINNQVVDDEEDDEEMLSGLENDSKQDLEGNDDGGED
EEDDDDDDEDDDDDEDEREDDDEQEDDAARMDKTATPTNGHQHDEQKAAAAGAGGAGG
AGDSGDAVTKIGSEDVKLSDVDGGVGSREASSSTHEASANGEVYEYYKHMLHAAQIAD
SYNIYPTAAIPIQTHVNALAVSRGLKYLFLGGSDGYIRKYDLLNTLEGKLSLTILQKH
SLAESIQNAGILQSYWENEIPQKKSEMKLSANKTDYEPKVSPVHSLEVQSECLFILSG
LQNGGITMQGVRYMEGSIAHYFKGRNGHTQIVNILRLNGQEDRFLSGSWDKRLLEWDL
QTGDIVNEFKKSRSELSSLEMRPLYSSVDVSGNVNSGKENENADDDMDSLFGDEDEDE
KQDAGNEPVEAGDGSNGEENKEQISEESLNIVYDESVFMTSGLNGSVHIWDRRMTQSP
ALSLERGAGVPPWCLSACWGVDGDHVYAGRRNACVEQFDLKMPSKPIHNLKLPSISGP
VSCVKAMPNNKHLLCASRDNIRLYNVEIAVDASNSTTKSSKVPFLIVPGHHGGIISNL
YLDPTSRFIISTSGNRGWQGNSTDTTLIYDIDLE"
gene 232497..233615
/gene="Erg3"
/locus_tag="R008_L10746"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 233963..236512
/locus_tag="R008_L10751"
mRNA 233963..236512
/locus_tag="R008_L10751"
/product="hypothetical protein"
CDS 233963..236512
/locus_tag="R008_L10751"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84330.1"
/translation="MSIARLVYSLFRRVRSVLLLFITISLLFYYTFQNEIDILNSYAL
NDSLPSINNYEHNTEGSSKLDPPDLSSTGSDRIATDKENGNVAVDLSDPATLREKNKY
FPLLLKGSSHQIGSNLPISSLLTYKEKYPVLFEYSSPSLTSTSQNDVHKIQPAMQLPP
DVDMIKQIKDIFMKSWNQEQLLLKSNLRRESTWPIDLIDSLDTLYLCGETKLFQDSVN
IIEDFDFRVPPLAMEVIDIPDITTRVLEGLLSAYELSMDKRLLNKAKHVADFILRSFD
TPNRIPILKYFWKSDLRNRFPDRTVPSGQLTTMALAFIRLSQLTRLNKYFDAVERVFT
TIRQSYNEFDMEFMLPDVVDASGCQLLTEEEIENGAHLKGSSIMKSINENFKFVHCQQ
LGKFLNPPMDDNSLRGQSQYQAYRINEKTVPILENLFKINDLFQSSYDILDGSSKNTN
AATMDPSIGSEVEAVDEIIEKRNFKDGAKKDSTKNTVGDKSLIDSQTFLTNSISNIFK
FMTFRPMLPKQTENKKFNFLNSILTKSQFMPTTNELDVTIRKSYDVSLYSCRLGGILG
LSSRVPHRGGVNTKYILPSSLLEMSEIITQSCFMLMEEFDGLLPQKFELDPCTDETNG
NCEFNGETKSRMIANGEYETFENDLDVGIKVSNYGKGGNDQKAKRNLLSKDGVTETQN
IKGDTVGSSKSIAEIDGDEVTQIRRVFTLGKDIKPHITTDDTMGSQWKNHPDWPFWVN
KVESRRLLDSNIIESIFYMYRISGEQKWRSMGKQSFGILMQELMELNSGAKGLWQIKE
FYENGEKVNNDLPSYWFSRTLKYYLLLFSDGDKVSLDKHILTQGGHIIKKK"
gene complement(236649..238058)
/gene="Shm2"
/locus_tag="R008_L10756"
mRNA complement(236649..238058)
/gene="Shm2"
/locus_tag="R008_L10756"
/product="Shm2p"
CDS complement(236649..238058)
/gene="Shm2"
/locus_tag="R008_L10756"
/note="Similar to YLR058C 'Cytosolic serine
hydroxymethyltransferase, converts serine to glycine plus
5,10 methylenetetrahydrofolate; major isoform involved in
generating precursors for purine, pyrimidine, amino acid,
and lipid biosynthesis'"
/codon_start=1
/product="Shm2p"
/protein_id="EWG84331.1"
/translation="MPYTLSDAHHKLITSHLVDTDPEVDSIIKDEIERQKHSIDLIAS
ENFTSTSVFDALGTPLSNKYSEGYPGARYYGGNEHIDRMEILCQQRALKAFHVTPDKW
GVNVQTLSGSPANLQVYQAIMKPHERLMGLYLPDGGHLSHGYATENRKISAVSTYFES
FPYRVNPETGIIDYDTLEKNAILYRPKVLVAGTSAYCRLIDYKRMREIADKCGAYLMV
DMAHISGLIAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRRGVRSINPKTGKEVL
YDLENPINFSVFPGHQGGPHNHTIAALATALKQAATPEFKEYQTQVLKNAKALESEFK
NLGYRLVSNGTDSHMVLVSLREKGVDGARVEYICEKINIALNKNSIPGDKSALVPGGV
RIGAPAMTTRGMGEEDFHRIVQYINKAVEFAQQVQQSLPKDACRLKDFKAKVDEGSDV
LNTWKKEIYDWAGEYPLAV"
gene complement(238395..239204)
/gene="Rex2"
/locus_tag="R008_L10761"
mRNA complement(238395..239204)
/gene="Rex2"
/locus_tag="R008_L10761"
/product="Rex2p"
CDS complement(238395..239204)
/gene="Rex2"
/locus_tag="R008_L10761"
/note="Similar to YLR059C '3'-5' RNA exonuclease; involved
in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S
rRNAs, and RNase P and RNase MRP RNA; localized to
mitochondria and null suppresses escape of mtDNA to
nucleus in yme1 yme2 mutants; RNase D exonuclease'"
/codon_start=1
/product="Rex2p"
/protein_id="EWG84332.1"
/translation="MKWLLFPARIVARTRPNFFSLYRRSVSQYLRPRTIQNLQSMAQT
PELKTKLFKPLVWIDCEMTGLDHVNDRIIEICCIITDGHLAPVKAADGQGDSHYESVI
HYGPEVMNKMNEWCIEHHGNSGLTAKVLASEKTLAQVEDELLEYIQRYIPDKNVGVLA
GNSVHMDRLFMVREFPKVIDHLFYRIVDVSSIMEVARRHNPALQARNPKKEAAHTAYS
DIKESIAQLQWYMDNYLKPPQETESVESIGSEQPESPSSSTSSFKRQRTDF"
gene 239637..241424
/gene="Frs1"
/locus_tag="R008_L10771"
mRNA 239637..241424
/gene="Frs1"
/locus_tag="R008_L10771"
/product="Frs1p"
CDS 239637..241424
/gene="Frs1"
/locus_tag="R008_L10771"
/note="Similar to YLR060W 'Beta subunit of cytoplasmic
phenylalanyl-tRNA synthetase, forms a tetramer with Frs2p
to generate active enzyme; able to hydrolyze
mis-aminoacylated tRNA-Phe, which could contribute to
translational quality control'"
/codon_start=1
/product="Frs1p"
/protein_id="EWG84333.1"
/translation="MPTVSVNKQQLFDLLGKDYTSQEFDELCFEFGMEMDEDTTEEAL
KTGEEPELKLDISANRYDLLCIEGISQSLNEYLERKERPDYKLSKPTTKLIIDKSTEQ
IRPFATAAVLRNIKLNEKSYASFIALQDKLHANLCRNRSLVAMGTHDLDSIEGPFHYR
ALPPKDIKFVPLNQTQEFTGDKLIEFYKSPEQKNNIGRYVHIIEDSPVFPVIMDSKDR
VCSLPPLINSEHSKISVNTRNILIDITATDKTKAEIVLNILTTMFSRYCDEPFTVEPV
EIVSEHNGQSRLAPNFNDRIMDVSIKYINSCLGLDQSADEIAHCLKKMSLHAVQSKED
KDILHVDIPVTRPDILHACDIMEDAAVGYGFNNLPKGEKLSNANFIAKPLPINKVSDI
FRVASSQATWVEVLPLTLCSHDENFKFLRQSDNGDLAVKLANPKTLEYQVVRTTLLPG
ILKTVKENRKHSLPIKVFETGDVVFKDDKLERKAYNERHWAAIYVGKNSGFEIIQGLL
GKIMQTFRTEWIADYGAAASGRGYWIEEDDSVKTYFPGRGAKVMFRSKEGAEPKQIGH
LGVLHPEVMMNFDVPFAASFVEVNAEVFL"
gene 241862..242624
/gene="Rpl22a"
/locus_tag="R008_L10776"
mRNA join(241862..241873,242271..242624)
/gene="Rpl22a"
/locus_tag="R008_L10776"
/product="Rpl22ap"
CDS join(241862..241873,242271..242624)
/gene="Rpl22a"
/locus_tag="R008_L10776"
/note="Similar to YLR061W Protein component of the large
(60S) ribosomal subunit, has similarity to Rpl22Bp and to
rat L22 ribosomal protein"
/codon_start=1
/product="Rpl22ap"
/protein_id="EWG84334.1"
/translation="MAPNTSRKQKIAKTFTVDVSSPTENGVFDPASYAKYLIDHIKVE
GAVGNLGNAVTVTEDGTVVTVVSTAKFSGKYLKYLTKKYLKKNQLRDWIRFVSTKTNE
YRLAFYQVTPEEDEEEDEE"
gene 242827..243924
/locus_tag="R008_L10786"
mRNA 242827..243924
/locus_tag="R008_L10786"
/product="hypothetical protein"
CDS 242827..243924
/locus_tag="R008_L10786"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84335.1"
/translation="MLLMRRFAFLTSSVYFKYIPIYSQYHYSSQFPINMNPKKVAQLP
VHNKSTLPPQEIIDLFKITFLEELYPKDQDNEKSPLTEQIQAVKSDLYNRDYNAAFNN
DSKRIAYCCRWSPSRATSYASVFAHFPELLKIIRCEIDDKDSNVLCIGGGAGGELVAL
ASIFTLSRDFSSKFASALKIDNEVNKKPRNLNIQLVDIADWSTVVEKLTATIKSKWLY
GDSEAESFNVNFTHKDCLQMTEPQDIKIYQGLDLITLLFTTNELFTQKKVESIKFLQR
LNENCAPGCHLLILESAGSYSHITINNKKFPIQFLIDTILVGNRKDKGTTGPWSLVSE
NDSIWYRMDPKLDYSIPLENMRFFYRLYVKN"
gene 244126..244947
/locus_tag="R008_L10791"
mRNA 244126..244947
/locus_tag="R008_L10791"
/product="hypothetical protein"
CDS 244126..244947
/locus_tag="R008_L10791"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84336.1"
/translation="MTVPRNRPMAPFGTIIKSRIKQPQFYWFIGHFLTIFNFIQFHLS
ITSKQNQLSCYRRSLFYISVTYAIVLYQFFKSDQLKFNFTLLRQEMKKLDNLQYFAML
FILFLLSQFNIIISGSLYSPVIFSIFHFLNYFKENLLPFLPLIPLNLKNLLNSKITVF
IQNYNGFFLQMAQVFEIICGLRVGLFLVPFNFFLLLVRRANVSFEVVGTMLAGLTYVW
FFKLRYLQSESMRQIFKQYVLRLDAYVSRTLPPYCSRLWNGYKNFVMTVFWKIPV"
gene complement(245043..245588)
/locus_tag="R008_L10796"
mRNA complement(245043..245588)
/locus_tag="R008_L10796"
/product="hypothetical protein"
CDS complement(245043..245588)
/locus_tag="R008_L10796"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84337.1"
/translation="MAGKAGKKQASSNAKIIQGLYKQVSLFLGMAIVRLFISRKVTIG
QWIKLVALNVPMFVALYIIVLSGKPKYDGNRVVKQGIDLNDNTNLISYFFDLIYLSLF
GNIGIIAFRTFKFWWCLLLCPIYAGYKLYGLKNMFMPGAQQTQADNRSKNANEGQSKS
KRQMKRERRGETDSKIKYKYR"
gene 245839..246393
/gene="Spc3"
/locus_tag="R008_L10801"
mRNA 245839..246393
/gene="Spc3"
/locus_tag="R008_L10801"
/product="Spc3p"
CDS 245839..246393
/gene="Spc3"
/locus_tag="R008_L10801"
/note="Similar to YLR066W 'Subunit of signal peptidase
complex (Spc1p, Spc2p, Spc3p, Sec11p), which catalyzes
cleavage of N-terminal signal sequences of proteins
targeted to the secretory pathway; homologous to mammalian
SPC22/23'"
/codon_start=1
/product="Spc3p"
/protein_id="EWG84338.1"
/translation="MFSFVQRFQNVSNQAFSMGIVMVVFIMASSYYQLINNNAFSVPS
NIDNVKTLINVRTSRYFGSQRGKAKENMKIKFDLNTDLTPLFNWNTKQVFVYLTAEYN
STEKITSEVTFWDKIIKSKDDAVIDVNDLRSKYSIWDIEDGKFEGKDLVFKLHWNVQP
WVGLLTYGETVGNYTLTVENKNKV"
gene complement(246483..249380)
/gene="Pet309"
/locus_tag="R008_L10806"
mRNA complement(246483..249380)
/gene="Pet309"
/locus_tag="R008_L10806"
/product="Pet309p"
CDS complement(246483..249380)
/gene="Pet309"
/locus_tag="R008_L10806"
/note="Similar to YLR067C 'Specific translational
activator for the COX1 mRNA, also influences stability of
intron-containing COX1 primary transcripts; localizes to
the mitochondrial inner membrane; contains seven
pentatricopeptide repeats (PPRs)'"
/codon_start=1
/product="Pet309p"
/protein_id="EWG84339.1"
/translation="MKRCAPAVLRNYNYKKGIWSTGVPDHIRKLLRDKSTSPLCSQDE
RNLVSYFMARGSVPLKSVGSGLTKKATTSITSNSATTTFERQYLIKYLYRHQAYGNVI
KIAQKFLYTTIGSQRLLKQDASLPELKKFLLSLLILQRGIQLDQAISDIIQRFLLTQK
TMVIDLINSIFSRMVIMNMHEEAVYKWVKWMKLVNGHCEFTNYMENKIVLRNFLSFMR
QSNVRPDYLSYLKAIQLTQGPAIASQFATTLLFLLTYIRKFSSAEAVWNYKCEHNLPI
VSSDLTCILKTYCHMQKFNLVSSTYWKYPDAQHDQNQFDYLLVAHSRLHNWDALQQQF
NALFGIGKLPSIQHYGILMYTMARIGELDSVNKLYTQLLRRGMIPTYAVLQSLLYAHY
KVGDFAACFSHFELFKKYDITPSTATHTIMLKVYRGLNDLDGAFRILKRLSEDPSVEI
TEGHFALLIQMCCKTTNHLIAQELFNLMTEHYNIQHTGKSISALMDVYSESNRPTEAI
ALFEKHSKNLSWRDGLISVYNKAIKAYIGLRNANKCEELFDKITNSKLAVNSEFYKMM
IKFLVTLNEDCETALSIIDQLIKHSVIKVDATHFEIIMEAYDKEGYRDGIINLYKTMS
QNKVPANSKILYYILKAVAKKSLQNNEEIKETINMVEDIMENAANGTLDVTYNKLHPS
VMAWPMRMIVKHDSPQRALELYNRYNELFFKKHDWVSNNNKFVMMRSLLVLLAQIEQW
KDFETLFAKYMDRIENIENLPSSTTPNIKLRSIFSGLFPYKVSQLIAMNKIDELPLLW
KKLREKGFILDNISWNSAVEALFKDPRTLSYGMKIVDDTLIHGYNLIHKFRLLTKLSE
DKTQSSDKSWPTLKMKEKEPNKFQPRLYLKSDTYNSIMRQLDTYLDGIDDLKTLEDQV
RDFISNYKYFMKDYLLMPRSKINKWEQIEMRHLSYFKELRKSKRVLPVSKF"
gene 249678..250133
/gene="Fyv7"
/locus_tag="R008_L10811"
mRNA 249678..250133
/gene="Fyv7"
/locus_tag="R008_L10811"
/product="Fyv7p"
CDS 249678..250133
/gene="Fyv7"
/locus_tag="R008_L10811"
/note="Similar to YLR068W 'Essential protein required for
maturation of 18S rRNA; required for survival upon
exposure to K1 killer toxin'"
/codon_start=1
/product="Fyv7p"
/protein_id="EWG84340.1"
/translation="MGTAKQNQNRKKFTREYKVKEIQRSITKKTRLRKEYLKALKDEG
YAVPEKEPKTVAKESVRKIKEARAIEGKKKLDEKKEIKKQRKRMQKDELNKQRSEQLE
RIRVSKEKFQRREDRKKKLTQRTRTGQPLMGPKIEDLLDKIKTDDTYTS"
gene complement(250300..252585)
/gene="Mef1"
/locus_tag="R008_L10816"
mRNA complement(250300..252585)
/gene="Mef1"
/locus_tag="R008_L10816"
/product="Mef1p"
CDS complement(250300..252585)
/gene="Mef1"
/locus_tag="R008_L10816"
/note="Similar to YLR069C Mitochondrial elongation factor
involved in translational elongation"
/codon_start=1
/product="Mef1p"
/protein_id="EWG84341.1"
/translation="MSVQKMMRVPRKMVGGRIPFFTCSKVFSGFSRRSFHESPLARST
YEEEKVLVDEIKQKLTPDDIERCNKLRNIGISAHIDSGKTTFTERVLYYTKRIKAIHE
VRGRDNVGAKMDSMDLEREKGITIQSAATYCSWDKEGKNYHFNLIDTPGHIDFTIEVE
RALRVLDGAVLVVCAVSGVQSQTVTVDRQMRRYNVPRVTFINKMDRMGSDPFRAIEQL
NSKLKIPAAAVQIPIGSESSLSGVVDLINRVAIYNKGDNGEIIEKGPVPENLKPLMEE
KRQLLIETLADVDDEMAEMFLEEKEPTTQQIKDAIRRSTIARSFTPVLMGSALANTGI
QPVLDAIVDYLPNPSEVLNTALDVSNNEAKVNLVPAVQQPFVGLAFKLEEGKYGQLTY
VRVYQGRLRKGNYITNVKTGKKVKVARLVRMHSSEMEDVDEVGSGEICATFGIDCASG
DTFTDGSVQYSMSSMYVPDAVVSLSITPNSKDASNFSKALNRFQKEDPTFRVKFDPES
KETIISGMGELHLEIYVERMRREYNVDCVTGKPQVSYRESITIPADFDYTHKKQSGGA
GQYGRVIGTLSPVDDITKGNIFETAIVGGRIPDKYLAACGKGFEEVCEKGPLIGHRVL
DVKMLINDGAIHAVDSNELSFKTATMSAFRDAFLRAQPVIMEPIMNVSVTSPNEFQGN
VIGLLNKLQAVIQDTENGHDEFTLKAECALSTMFGFATSLRASTQGKGEFSLEFSHYA
PTAPHVQKELISEFQKKQAKK"
gene complement(252807..253877)
/gene="Xyl2"
/locus_tag="R008_L10826"
mRNA complement(252807..253877)
/gene="Xyl2"
/locus_tag="R008_L10826"
/product="Xyl2p"
CDS complement(252807..253877)
/gene="Xyl2"
/locus_tag="R008_L10826"
/note="Similar to YLR070C 'Xylitol dehydrogenase, converts
xylitol to D-xylulose; expression induced by xylose, even
though this pentose sugar is not well utilized by S.
cerevisiae; null mutant has cell wall defect'"
/codon_start=1
/product="Xyl2p"
/protein_id="EWG84342.1"
/translation="MTDLTTQEAIVLERPGKITLTNVSIPKISDPNEVIIQIKATGIC
GSDIHYYTHGRIANYVVESPMVLGHESSGIVALIGENVKTLKVGDRVALEPGIPDRFS
PEMKEGRYNLDPNLKFAATPPFDGTLTKYYKTMKDFVYKLPDDVSFEEGALIEPLSVA
IHANKLAKIKFGARCVVFGAGPIGLLAGKVASVFGAADVVFVDLLENKLERARQFGAT
HIVNSGDLPHGVTVDSVIKKAIGKKGADVVFECSGAEPCVRAGIEVCKAGGTIVQVGM
GQEEIQFPISIIPTKELTFQGCFRYCQGDYSDSIELVSSRKLSLKPLITHRYSFKDAV
EAFEETSHHPLNNIKTTIEGPE"
gene complement(254044..257292)
/gene="Rgr1"
/locus_tag="R008_L10831"
mRNA complement(254044..257292)
/gene="Rgr1"
/locus_tag="R008_L10831"
/product="Rgr1p"
CDS complement(254044..257292)
/gene="Rgr1"
/locus_tag="R008_L10831"
/note="Similar to YLR071C 'Subunit of the RNA polymerase
II mediator complex; associates with core polymerase
subunits to form the RNA polymerase II holoenzyme;
required for glucose repression, HO repression, RME1
repression and sporulation'"
/codon_start=1
/product="Rgr1p"
/protein_id="EWG84343.1"
/translation="MTTTIGSPQMLANEERLSNEMHALKNRSEQNGQEQQGPVKNTQL
HSPSATVPETTTTQKESLEMVPKDTSAATMTSAPPPALPHVEINQVSLALVIRNLTVF
TMKELAQYMKTNVHTQANEPNSAKKIRFLQLIIFLRTQFLKLYVLVKWTRTIKQNNFH
VLIDLLNWFRTTNMNVNNCIWALKSSLNSMTNAKLPNVDLVTALEVLSLGRPNLPTHN
FKLSGVSNSMDMVDGMAKVPIGLILQRLKDLNLTVSIKIALMNIPKPLNSYHIKNGRI
YFTVPNEFEIQLSTVNRQSPLFFVDLKLLFNTEAEQTVSAVTEATSTNGDSENNEENS
SSNGNNLPLNKPRLEKLINEILLKSNDPLLSLYNFLHKYVLTLQLYMVHREFLKLANG
GKFSKSNLIHNYDSKKSTITVRYWLNGKMDSKGKITIGIQRTTESLILKWDNQSASRA
KNMPVIYNNIVSNIEGILDEIMFNHARIIRSELLARDIFQEDEENSDVLLFQLPTTCV
SMAPIQLKIDLLSGQFYFRNPTPLLSNYASKINRAEGPEELARILQQLKLDKIIHVLT
TMFENTGWSCSRIIKIDKPIRTQVNTGGESIVKKEDNKYAIAGNNTTNSDVSLLLQRD
LFIRLPHWPLNWYLILSIISSKTSCVVEKRIGKIVSQRGKWHLKYLDNSNVMTVKLES
ITYQKIMILQRTILNRIINHMLIDSLNQLEIRNKICSSEMINEQKLPQYIIQGSNTND
NISIIALELESFLEGSKALNSILESSMFLRIDYSNSQIRLYAKFKRNTMMIQCQIDKL
YIHFVQEEPLAFYLEESFTNLGIIVQYLTKFRQKLMQLVVLTDVVERLHKNFESENFK
IIALQPNEISFKYLSNNDEDDKDCTIKISTNDDSIKNLTVQLSPSNPQHIIQPFLDNS
KMDYHFIFSYLQFTSSLFKALKVILNERGGKFHESGSQYSTMVNIGLHNLNEYQIVYY
NPQAGTKITICIELKTVLHNGRDKIQFHIHFADVAHITTKSPAYPMMHQVRNQVFMLD
TKRLGTPESVKPANASHAIRLGNGVACDPSEIEPILMEIHNILKVDSNSSSS"
gene 257528..259627
/locus_tag="R008_L10836"
mRNA 257528..259627
/locus_tag="R008_L10836"
/product="hypothetical protein"
CDS 257528..259627
/locus_tag="R008_L10836"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84344.1"
/translation="MWGDSMRELGDAMDNELNSVKPVVEEGGMDGARKFIKGKSFQKS
STEHMLISPGRDGSVPLNGLKSSPADPHLSDVNSILDNHRGGGETALTSVNNIIMATS
TNGDSDGVDGDAKRPSISNCSSRSSFFDTVLSTFSLKSNSQDTVTNEVKNIEVQFASE
EANIKFRQMFKPLAPNTRLITDYFCYFHREFPYQGRIYLSNTHLCFNSTVLNWMAKLQ
IPLNEIKYLDKVTTNSSAISVETVTNRYTFSGFIARDEVFQLITRVWSKENLTNINDV
LEVDERVSKKKGISSTPSSIFNNVSTNAYNDFISTTTTEPTSRASYMSENDMLIEEAI
RSVDDYMGTPRASPSSSSSSSSSSSSLGSSTTYYCRPVYRLKPNAPFQYEGPFHVQET
MDFPYKPEANNEYVLLERQFSVPPGLLFIMMFNEDNPVFELSFLKTQDSSNISRIGTF
EKVNKDGQHYREFQYTKQLHFPVGPKSTNCEVAEILLHCDWERYINVLSITRTPNVPS
GTSFSTRTRYMFRWDDQGQGCILKISFWVDWNASSWIKPMVESNCKNGQISATKDLVK
LVEEFVEKYVELSKEKADTLKPLPSVTSFGSPRKVAAPELTMVQPESKPEAEVEISEI
GSDRWRFNWVNIIILVLLVLNLLYLMKLNKKMDKLTNLMTHKDEVVAHATLLDIPAKV
SQSTMVKTKKGRRVVTE"
gene complement(259650..260312)
/locus_tag="R008_L10841"
mRNA complement(259650..260312)
/locus_tag="R008_L10841"
/product="hypothetical protein"
CDS complement(259650..260312)
/locus_tag="R008_L10841"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84345.1"
/translation="MKSSKQLVQDAKDYRFNPAIPLRIYLKTCIGILEKAQCAFQAND
LSLSFIYYFRYVDLLTNKLSRHPELLRMDASSSSSSSYIHKREYLQLIKLEVPAVCKI
IESLRTQIDSQYSKLQTSLANNIAKPNINANTTPVQVEQQPLPKKSFDEYSFNQSISF
FQKISNAQLNTGASSQSQATARDEAYRLNYPELSGATTPYILHIAFPFFFFFFPIPYE
HT"
gene complement(260645..261145)
/gene="Bud20"
/locus_tag="R008_L10851"
mRNA complement(260645..261145)
/gene="Bud20"
/locus_tag="R008_L10851"
/product="Bud20p"
CDS complement(260645..261145)
/gene="Bud20"
/locus_tag="R008_L10851"
/note="Similar to YLR074C 'Protein involved in bud-site
selection; diploid mutants display a random budding
pattern instead of the wild-type bipolar pattern'"
/codon_start=1
/product="Bud20p"
/protein_id="EWG84346.1"
/translation="MGRYSVKRYKTKRRTRDLDMIYNDLSTKESVQKLLNQPLDETKP
GLGQHYCIHCAKYMETAIALKTHLKGKVHKRRVKELKGVPYTQEVSDAAAGYNLNKFL
NRVQEITQSVGPEKESNEALLKEHLDSTLANVKTTEPTLPWAAADAEANTAAVTEAES
TASAST"
gene 261612..262277
/gene="Rpl10"
/locus_tag="R008_L10856"
mRNA 261612..262277
/gene="Rpl10"
/locus_tag="R008_L10856"
/product="Rpl10p"
CDS 261612..262277
/gene="Rpl10"
/locus_tag="R008_L10856"
/note="Similar to YLR075W 'Protein component of the large
(60S) ribosomal subunit, responsible for joining the 40S
and 60S subunits; regulates translation initiation; has
similarity to rat L10 ribosomal protein and to members of
the QM gene family'"
/codon_start=1
/product="Rpl10p"
/protein_id="EWG84347.1"
/translation="MARRPARCYRYQKNKPYPKSRYNRAVPDSKIRIYDLGKKKATVD
EFPLCVHLVSNELEQLSSEALEAARICANKYMTTVSGRDAFHLRVRVHPFHVLRINKM
LSCAGADRLQQGMRGAWGKPHGLAARVDIGQIIFSVRTKDSNKDVVVEGLRRARYKFP
GQQKIILSKKWGFTNLDRPEYLKKREAGEVKDDGAFVKFLSKKGSLENNIREFPEYFA
AQA"
gene 262557..264308
/gene="Fmp25"
/locus_tag="R008_L10866"
mRNA 262557..264308
/gene="Fmp25"
/locus_tag="R008_L10866"
/product="Fmp25p"
CDS 262557..264308
/gene="Fmp25"
/locus_tag="R008_L10866"
/note="Similar to YLR077W 'Mitochondrial inner membrane
protein required for an early step in assembly of
respiratory complex III (cytochrome bc1 complex); mRNA is
targeted to mitochondria'"
/codon_start=1
/product="Fmp25p"
/protein_id="EWG84348.1"
/translation="MSFRLFTRTSQRLPRLNWVSPIRRYAKQPQYDEAELFAENINHG
AYKAKKRPSDEHFQWPEKSPDQITKESELQWERMAKLSAVGQGILILVVVGGLGTAYL
RWPELKSWWLIKMNGGRINATQEQSGQDSLEKLIRQKAKNLLREIPQVPAFQLGIDHP
GVYIWGRCHSKNSLFPVRVPNLDGRKFRDILLAPSDDFNTNFAIDEKGDLISWDDLGQ
TKTILPDQDLTSMKYSSHFLYALNKKGEILVIPIRTPDLIASQVSSRRSKLLPWKTKL
RYDWKLQTNQIFNGKEGEKRVIQFDAGSHHLVLLSNLGKAYCCATGNDQKQAQVSKGQ
FGIPTFSQFDEFPPNNQLFEIELLNKFKHEGEDVVRKREIKKIACGSYHTLAIDKTGE
IYAFGWNRFGQLALPISYNLEYVSFPRSVTYAFKPHFPGMTNWKCVDIHCDDETSFVT
IRKPGSTSDHHYFAFGNGLFGELGNNTFKNSQCDPIKIKSDDKKLTNWSCGSHCVFTE
TEQENEVIAWGNNDHGQLGIGKKTMKCAKPMNIPEVLKPGQDTTDLDSIYNSKLHLKK
EQRVVTNGNKSCLYWRV"
gene complement(264421..265244)
/gene="Bos1"
/locus_tag="R008_L10871"
mRNA complement(join(264421..265152,265242..265244))
/gene="Bos1"
/locus_tag="R008_L10871"
/product="Bos1p"
CDS complement(join(264421..265152,265242..265244))
/gene="Bos1"
/locus_tag="R008_L10871"
/note="Similar to YLR078C v-SNARE (vesicle specific SNAP
receptor), localized to the endoplasmic reticulum membrane
and necessary for vesicular transport from the ER to the
Golgi"
/codon_start=1
/product="Bos1p"
/protein_id="EWG84349.1"
/translation="MNALYNHAVKQKNQLQQELARFEKNSVTAPISLQGSISATLVSL
EKTVKQYAEHLNRYKEDTNAEEIDPKFANRLATLTQDLHDFTAKFKDLKQSYNENNSR
TQLFGSGASHVMDSDNPFSTSETIMNKRNVGGASANGKEGSSNGGGLPLYQGLQKEQS
VFERGNAQLDYILEMGQQSFENIVEQNKILSKVQDRMSNGLRTLGVSEQTITSINKRV
FKDKLVFWIALILLIIGIYYVLKWLR"
gene 265505..266359
/gene="Sic1"
/locus_tag="R008_L10876"
mRNA 265505..266359
/gene="Sic1"
/locus_tag="R008_L10876"
/product="Sic1p"
CDS 265505..266359
/gene="Sic1"
/locus_tag="R008_L10876"
/note="Similar to YLR079W 'Inhibitor of Cdc28-Clb kinase
complexes that controls G1/S phase transition, preventing
premature S phase and ensuring genomic integrity;
phosphorylation targets Sic1p for SCF(CDC4)-dependent
turnover; functional homolog of mammalian Kip1'"
/codon_start=1
/product="Sic1p"
/protein_id="EWG84350.1"
/translation="MTPSTPPRSRGTRYLAQPSGNTSSSALMQGQKTPQKPSQNLVPV
TPSTTKSFKNAPLLAPPNSSMGMTSPFNGLTSPQRSPFPKSSVKRTLFQFEIHDNGTV
REEQEPLGRVNRILFPTQQNVDIDAAEEEEEGEVLLPPSRPTSARQLHLSLERDEFDQ
THRKKIIKDVPGTPSDKVITFELAKNWNNNSPRNDAKSQESEDEEDIIINPVRVGKNP
FASDELVTQEIRNERKRAMLRENPDIEDVITYVNKKGEVVEKRRLTDEEKRRFKPKAL
FQSRDQEH"
gene 266601..267935
/gene="Emp46"
/locus_tag="R008_L10886"
mRNA 266601..267935
/gene="Emp46"
/locus_tag="R008_L10886"
/product="Emp46p"
CDS 266601..267935
/gene="Emp46"
/locus_tag="R008_L10886"
/note="Similar to YLR080W Integral membrane component of
endoplasmic reticulum-derived COPII-coated vesicles, which
function in ER to Golgi transport"
/codon_start=1
/product="Emp46p"
/protein_id="EWG84351.1"
/translation="MTTRKTGSSLQLLGKITGTKAGTKQKKMNFINGLIWLYMCVWMV
HGKVTQKDELKWNKGYSLPNLLEVTDQQKELSQWTLGDKVKLEEGRFVLTPGKNTKGS
LWLKPEYSIKDAMTIEWTFRSFGFRGSTKGGLAFWLKQGNEGDSTELFGGSSKKFNGL
MILLRLDDKLGESVTAYLNDGTKDLDIESSPYFASCLFQYQDSMVPSTLRLTYNPLDN
HLLKLQMDNRVCFQTRKVKFMGSSPFRIGTSAINDASKESFEILKMKLYDGVIEDSLI
PNVNPMGQPRVVTKVINSQTGEESFREKMPFSDKEESITSNELFEKMNKLEGKIMAND
IDPLLRKMNKIVENERELIQRLRPLLDLKKTAISDDSFQDFLSMNANLDRLIKEQEKI
RQDAKLYGKQTKGHDEIFSKISVWLALLIFIMITLAYYMFRINQDIKKVKLL"
gene 268895..270619
/gene="Gal2"
/locus_tag="R008_L10911"
mRNA 268895..270619
/gene="Gal2"
/locus_tag="R008_L10911"
/product="Gal2p"
CDS 268895..270619
/gene="Gal2"
/locus_tag="R008_L10911"
/note="Similar to YLR081W 'Galactose permease, required
for utilization of galactose; also able to transport
glucose'"
/codon_start=1
/product="Gal2p"
/protein_id="EWG84352.1"
/translation="MAVEENNMPVVSQQPQAGEDVISSLSKDSHLSAQSQKYSNDELK
AGESGPEGSQSVPIEIPKKPMSEYVTVSLLCLCVAFGGFMFGWDTGTISGFVVQTDFL
RRFGMKHKDGTHYLSNVRTGLIVAIFNIGCAFGGIILSKGGDMYGRKKGLSIVVSVYI
VGIIIQIASINKWYQYFIGRIISGLGVGGIAVLCPMLISEIAPKHLRGTLVSCYQLMI
TAGIFLGYCTNYGTKSYSNSVQWRVPLGLCFAWSLFMIGALTLVPESPRYLCEVNKVE
DAKRSIAKSNKVSPEDPAVQAELDLIMAGIEAEKLAGNASWGELFSTKTKVFQRLLMG
VFVQMFQQLTGNNYFFYYGTVIFKSVGLDDSFETSIVIGVVNFASTFFSLWTVENLGR
RKCLLLGAATMMACMVIYASVGVTRLYPHGKSQPSSKGAGNCMIVFTCFYIFCYATTW
APVAWVITAESFPLRVKSKCMALASASNWVWGFLIAFFTPFITSAINFYYGYVFMGCL
VAMFFYVFFFVPETKGLSLEEIQELWEEGVLPWKSEGWIPSSRRGNNYDLEDLQHDDK
PWYKAMLE"
gene complement(271074..272252)
/gene="Srl2"
/locus_tag="R008_L10916"
mRNA complement(271074..272252)
/gene="Srl2"
/locus_tag="R008_L10916"
/product="Srl2p"
CDS complement(271074..272252)
/gene="Srl2"
/locus_tag="R008_L10916"
/note="Similar to YLR082C 'Protein of unknown function;
overexpression suppresses the lethality caused by a rad53
null mutation'"
/codon_start=1
/product="Srl2p"
/protein_id="EWG84353.1"
/translation="MSNFKNFTLNSFEDYYGKPSETPKMEEEKLEVTNVNASSSKKVH
KSKKSTSKYDQKNVFRNSMTGIAQILPTKPVKIIEQNIDFANPKSFDLLQSTHTICFN
KRINTTNTKLNVETHTSSDIDNDILHVGVPTDLGGNSNDEAETRQLRKFRWSNNKEKS
LCEKLTVIYWALLLHTTKRASKRRPILCHQMIAEFFNRVYKEKSRVPITSRYIRDNLV
AWVTQGKELHEKGWVGDAKTSDLQEQFNIATVKLYESAEDGRLSIGKDKPFREENTGS
DSLVRAEEDSTAVTNENGHISSEKNLKKDRRESIRNQILTLDVNDEDFFQNVMKVLSA
IDEPELRQYVIVISELVTMEMDDGKTVREKLRDVELNINRLQVDIKEIKEMLVTLINK
"
gene complement(272770..274773)
/gene="Emp70"
/locus_tag="R008_L10921"
mRNA complement(272770..274773)
/gene="Emp70"
/locus_tag="R008_L10921"
/product="Emp70p"
CDS complement(272770..274773)
/gene="Emp70"
/locus_tag="R008_L10921"
/note="Similar to YLR083C 'Protein with a role in cellular
adhesion, filamentous growth, and endosome-to-vacuole
sorting; similar to Tmn2p and Tmn3p; member of
Transmembrane Nine family of proteins with 9 transmembrane
segments'"
/codon_start=1
/product="Emp70p"
/protein_id="EWG84354.1"
/translation="MIYKMAHVQLLLLYFFVSTVKAFYLPGVAPTTYRENDNIPLLVN
HLTPSMNYQHKDEDGNNVSGDKENFLYSYDYYYNRFHFCQPEKVEKQPESLGSVIFGD
RIYNSPFQLNMLQEKECESLCKTVIPGDDAKFINKLIKNGFFQNWLIDGLPAAREVYD
GRTKTSFYGAGFNLGFVQVTQGTDIEATPKGAETTDKDVELETRNDRNMVKTYELPYF
ANHFDIMIEYHDRGEGNYRVVGVIVEPVSIKRSSPGTCETTGSPLMLDEENDNEVYFT
YSVKFNESATSWATRWDKYLHVYDPSIQWFSLINFSLVVVLLSSVVIHSLLRALKSDF
ARYNELNLDDDFQEDSGWKLNHGDVFRSPSQSLTLSILVGSGVQLFLMVTCSIFFAAL
GFLSPSSRGSLATVMFILYALFGFVGSYTSMGIYKFFNGPYWKANLILTPLLVPGAIL
LIIIALNFFLMFVHSSGVIPASTLFFMVFLWFLFSIPLSFAGSLIARKRCHWDEHPTK
TNQIARQIPFQPWYLKTIPATLIAGIFPFGSIAVELYFIYTSLWFNKIFYMFGFLFFS
FLLLTLTSSLVTILITYHSLCLENWKWQWRGFIIGGAGCALYVFIHSILFTKFKLGGF
TTIVLYVGYSSVISLLCCLVTGSIGFISSMLFVRKIYSSIKVD"
gene complement(275268..278930)
/gene="Rax2"
/locus_tag="R008_L10926"
mRNA complement(275268..278930)
/gene="Rax2"
/locus_tag="R008_L10926"
/product="Rax2p"
CDS complement(275268..278930)
/gene="Rax2"
/locus_tag="R008_L10926"
/note="Similar to YLR084C 'N-glycosylated protein involved
in the maintenance of bud site selection during bipolar
budding; localization requires Rax1p; RAX2 mRNA stability
is regulated by Mpt5p'"
/codon_start=1
/product="Rax2p"
/protein_id="EWG84355.1"
/translation="MFVHRLWTLAFPFLVEISKASQLENIKSLLDIEDNVLPNLNISQ
NNSNAVQILGGVDALSFYEYTGQQNFTKEIGPETSSHGLVYYSNNTYIQLEDASDDTR
IDKITPFGVDSFILSGSGTINNISVGNQILYNLSTLSMTPIFNQSLGAVQAVLADNSS
IYFGGNFSYNNGFMTGYSALIWDSISNTTQLLPFGGFGENSSVNSIVKLNNDNILFAG
QFYTLDDPSALISSSNNGTNSTSSLNATTLELGQRIPLRYASWDSQGSTTFASDSLVC
PNTNEDAWLYPDTSGSLVCNLPYEVSPTKIRLYNSQSSDSEISVFQILTDPSSSIMNL
TYLDPLSGELKNCDEFCPLYSRATLLSASQNVSSSMDMITFIDNNKTDVKWTSDFQDF
AFVNELPVSSLKFVALNSYGGSVGLSGLELYQDTFSTYANDSLNEYGCSALTNDSSSS
TLSSNDWYNGLTGESYIAAKYVPDQNEPIPRVKFYPNIIHPGHYTINMYTPGCLQDNT
CSARGIVNVTMWNQQNNTIMKTYLIYQNNDNLKYDQIYSGYLDFSPEIVLEYVSGIYT
TNTATVVVADQVNVITVSLDAFNTLSDSSNAKKETLLNGILQYQKSNFTSTRLNETKV
GNTTLNLFPVKNYPKNSSLYADIYDNKLVIGGVSNRISIVDLNDDFEVTSSKNQTIQG
DVHGITKTNQGLLIFGDILSSNNQSAVFLFNGSFENVFNQSRTVNSALNISLANNDFI
VLDNDYVVNASSNALIRNSSSFSLSLWAAGNNGDGDVLFSGAVSHMQYGNLNGSVRFL
NENEIEPLNLEGGIVPYLGAYLNESATAYAYEVDSLNKIYFSNEVYPSWNWSSGITQM
LYADNQTLLAVSAGSSTTAELSIFDLRNLTMIANETLGSNARINALVNFEKNCSMLVG
GDFQMTEPNCTGLCLYNYESKTWSTFLNNTIFGEITQLSFTNSSELIISGLFETKEYQ
SIRLGSFNLTNSTMIPLLSGSEGKLNSFTVTEDSIVAWNDTSLFIYRNQEWNITSLPG
NDSSISSVSAIYTDIESNTLNKRGINNVNNGSILLLNGNFNISQYGYLQSMLFDFQKW
TPYFISETTNTSNYNPIIFINRDVSTEFNSQSPLANVNITVTSPQSTSSQPPSSSASS
ESKSKSKKKKIGRGFVVLIGLALALGTVSVLGIAGVILAYVFKDPEGDYKPIKPRIDE
NEMLDTVPPEKLMKFV"
gene complement(279352..280668)
/gene="Arp6"
/locus_tag="R008_L10931"
mRNA complement(279352..280668)
/gene="Arp6"
/locus_tag="R008_L10931"
/product="Arp6p"
CDS complement(279352..280668)
/gene="Arp6"
/locus_tag="R008_L10931"
/note="Similar to YLR085C 'Actin-related protein that
binds nucleosomes; a component of the SWR1 complex, which
exchanges histone variant H2AZ (Htz1p) for chromatin-bound
histone H2A'"
/codon_start=1
/product="Arp6p"
/protein_id="EWG84356.1"
/translation="METPPIVIDNGSYEIKFGPSTNKKPFRALNALAKDKFGTSYLSN
HIKNIKDISSITFRRPHELGQLTLWELESCIWDYCLFNPSEFDGFDLKEGKGHHLVAS
ESCMTLPELSKHADQVIFEEYEFDSLFKSPVAVFVPFTKSYKGEMRTISGKDEDIDIV
RGNSDSTNSTSSESKNAQDSGSDYHDFQLVIDSGFNCTWIIPVLKGIPYYKAVKKLDI
GGRFLTGLLKETLSFRHYNMMDETILVNNIKEQCLFVSPVSYFDSFKTKDKHALGYVL
PDFQTSFLGYVRNPRKENVPLPEDAQIITLTDELFTIPETFFHPEISQITKPGIVEAI
LESLSMLPEIVRPLMVGNIVCTGGNFNLPNFAQRLAAELQRQLPTDWTCHVSVPEGDC
ALFGWEVMSQFAKTDSYRKARVTREEYYEHGPDWCTKHRFGYQNWI"
gene 280922..285178
/gene="Smc4"
/locus_tag="R008_L10936"
mRNA 280922..285178
/gene="Smc4"
/locus_tag="R008_L10936"
/product="Smc4p"
CDS 280922..285178
/gene="Smc4"
/locus_tag="R008_L10936"
/note="Similar to YLR086W 'Subunit of the condensin
complex; reorganizes chromosomes during cell division;
forms a complex with Smc2p that has ATP-hydrolyzing and
DNA-binding activity; required for tRNA gene clustering at
the nucleolus; potential Cdc28p substrate'"
/codon_start=1
/product="Smc4p"
/protein_id="EWG84357.1"
/translation="MSDSPLSKRQKRKAAQEPELSLDQGDAEEESQVENRVNLSENTP
EPDLPALEASYSKSYTPRKLVLSSGENRYAFSQPTNSTTTSLHVPNLQPPKTSSRGRD
HKSYSQSPPRSPGRSPTRRLELLQLSPVKNSRVELQKIYDSHQSSSKQQSRLFINELV
LENFKSYAGKQVVGPFHTSFSAVVGPNGSGKSNVIDSMLFVFGFRANKMRQDRLSDLI
HKSEAFPSLQSCSVAVHFQYVIDESSGTSRIDEEKPGLIITRKAFKNNSSKYYINEKE
SSYTEVTKLLKNEGIDLDHKRFLILQGEVENIAQMKPKAEKESDDGLLEYLEDIIGTA
NYKPLIEERMGQIENLNEVCLEKENRFEIVDREKNSLESGKETALEFLEKEKQLTLLR
SKLFQFKLLQSNSKLASTLEKISSSNKDLEDERMKFQESLKKVDEIKAQRKEIKDRIS
SCSSKEKTLVLERRELEGTRVSLEERTKNLVSKMEKAEKTLKSTKHSISEAENMLEEL
RGQQTEHETEIKDLTQLLEKERSILDDIKLSLKDKTKDISAEIIRHEKELEPWDLQLQ
EKESQIQLAESELSLLEETQAKLKKNVETLEEKILAKKTHKQELQDLILDLKKKLNSL
KDERSQGEKNFTSAHLKLKEMQKVLNAHRQRAMEARSSLSKAQNKSKVLTALSRLQKS
GRINGFHGRLGDLGVIDDSFDIAISTACPRLDDVVVDTVECAQHCIDYLRKNKLGYAR
FILLDRLRQFNLQPISTPENVPRLFDLVKPKNPKFSNAFYSVLRDTLVAQNLKQANNV
AYGKKRFRVVTVDGKLIDISGTMSGGGNHVAKGLMKLGTNQSDKVDDYTPEEVDKIER
ELSERENNFRVASDTVHEMEEELKKLRDHEPDLESQISKAEMEADSLASELTLAEQQV
KEAEMAYVKAVSDKAQLNVVMKNLERLRGEYNDLQSETKTKKEKIKGLQDEIMKIGGI
KLQMQNSKVESVCQKLDILVAKLKKVKSASKKSGGDVVKFQKLLQNSERDVELSSNEL
KVIEEQLKHTKLALAENDTNMTETLNLKVELKEQSEQLKEQMEDMEESINEFKSIEIE
MKNKLEKLNSLLTYIKSEITQQEKGLNELSIRDVTHTLGMLDDNKMDSVKEDVKNNQE
LDQEYRSCETQDESEIKDDETSCDNYHPMNVDETSDEVSRGIPRLSEDELRELDVELI
ESKINELSYYVEETNVDIGVLEEYARRLAEFKRRKLDLNNAVQKRDEVKEQLGILKKK
RFDEFMAGFNIISMTLKEMYQMITMGGNAELELVDSLDPFSEGVTFSVMPPKKSWRNI
TNLSGGEKTLSSLALVFALHKYKPTPLYVMDEIDAALDFRNVSIVANYIKERTKNAQF
IVISLRNNMFELAQQLVGVYKRDNRTKSTTIKNIDILNRT"
gene complement(285531..294407)
/gene="Csf1"
/locus_tag="R008_L10941"
mRNA complement(285531..294407)
/gene="Csf1"
/locus_tag="R008_L10941"
/product="Csf1p"
CDS complement(285531..294407)
/gene="Csf1"
/locus_tag="R008_L10941"
/note="Similar to YLR087C 'Protein required for
fermentation at low temperature; the authentic, non-tagged
protein is detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Csf1p"
/protein_id="EWG84358.1"
/translation="MEAISQLRGVPLTHQKDFSWVFLVDWILTVVVCLTMIFYMGRIY
AYLVSFILEWLLWKRAKIKINVETLRISLLGGRIHFKNLSVIHKDYTISVLEGSLTWK
YWLLNCRKAELIENDKSSSGKKAKLPCKISVECEGLEIFIYNRTVAYDNVINLLSKDE
RDKFEKYLNEHSFPEPFSDGSSADKLDEDLSESAYTTNSDASIVNDRDYQETDIGKHP
KLLMFLPIELKFSRGSLLLGNKFTPSVMILSYESGKGIIDVLPPKERLDLYRNKTQME
FKNFEISIKQNIGYDDAIGLKFKIDRGKVSKLWKTFVRVFQIVTKPVVPKKTKKSAGT
SDDNFYHKWKGLSLYKASAGDAKASDLDDVEFDLTNHEYAKFTSILKCPKVTIAYDVD
VPGVVPHGAHPTIPDIDGPDVGNNGAPPDFALDVQIHGGSICYGPWAQRQVSHLQRVL
SPVVSRTAKPIKKLPPGSRRIYTLFRMNISIMEDTTWRIPTRESSKDPEFLKHYKETN
EEYRPFGWMDLRFCKDTYANFNISVCPTVQGFQNNFHVHFLETEIRSSVNHDILLKSK
VFDIDGDIGYPLGWNSKAIWIINMKSEQLEAFLLREHITLVADTLSDFSAGDPTPYEL
FRPFVYKVNWEMEGYSIYLNVNDHNIVNNPLDFNENCYLSLHGDKLSIDVTVPRESIL
GTYTDMSYEISTPMFRMMLNTPPWNTLNEFMKHKEVGRAYDFTIKGSYLLYSELDIDN
VDTLVIECNSKSTVLHCYGFVMRYLTNVKMNYFGEFFNFVTSEEYTGVLGAREVGDVT
TKSSVADLASTVDSGYQNSSLKNESEDKGPMKRSDLKRTTNETDIWFTFSVWDGALIL
PETIYSFDPCIALHFAELVVDFRSCNYYMDIMAVLNGTSIKRHVSKQINEVFDFIRRN
NGADEQEHGLLSDLTIHGHRMYGLPPTEPTYFCQWDINLGDLCIDSDIEFIKGFFNSF
YKIGFGYNDLENILLYDTETINDMTSLTVHVEKIRIGLKDPVMKSQSVISAESILFTL
IDFENEKYSQRIDVKIPKLTISLNCVMGDGVDTSFLKFETKLRFTNFEQYKDIDKKRS
EQRRYITIHDSPYHRCPFLLPLFYQDSDTYQNLYGAIAPSSSIPTLPLPTLPDTIDYI
IEDIVGEYATLLETTNPFKNIFAETPSTMEPSRASFSEDDNEEGADPSSFKPVAFTED
RNHERDNYVVDVSYILLDVDPLLFIFAKSLLEQLYSENMVQVLDDIEIGIVKRLSNLQ
EGITSISNIDIHIAYLNLIWQETGEEGFELYLDRIDYQMSEKSLEKNRTNKLLEVAAL
AKVKTVRVTVNQKKNPDLSEDRPPALSLGIEGFEVWSSTEDRQVNSLNLTSSDITIDE
SQMEWLFEYCSDQGNLIQEVCTSFNSIQNTRSNSKTELISKLTAASEYYQISHDPYVI
TKPAFIMRLSKGHVRENRSWKIITRLRHILTYLPDDWQSNIDEVLKEKKYTSAKDAKN
IFMSVFSTWRNWEFSDVARSYIYGKLFTAENEKHKQNLIKKLLKCTMGSFYLTVYGEG
YEVEHNFVVADAKLVVDLTPPVTSLPSNREETIEITGRVGSVKGKFSDRLLKLQDLIP
LIAAVGEDDKSDPKKELSKQFKMNTVLLVDKSELQLVMDQTKLISRTVGGRVSLLWEN
LKDSTSQAGSLVIFSQKSEVWLKHTSVILGEAQLRDFSILATTEAWSHKPTILINNQC
ADLHFRAMSSTEQLVTAITEIRESLMMIKERIKFKPKSKKKSQFVDQKINTVLSCYFS
NVSSEVMPLSPFYIRHEAKQLDIYFNKFGSNEILLSIWDTDFFMTSHQTKEQYLRFSF
GDIEIKGGISREGYSLINVDISISMIKLTFSEPRRIVNSFLQDEKLASQGINLLYSLK
PLFFSSNLQKKEKQAPSIMINWTLDTSITYFGVLVPVASTYFVFELHMLLLSLTNTNN
GMLPEETKVTGQFSIENILFLIKERSLPIGLSKLLDFSIKVSTLQRTVDTEQSFQVES
SHFRVCLSPDSLLRLMWGAHKLLDLSHYYSRRHAPNIWNTKMFTGKSDKSKEMPINFR
SIHILSYKFCIGWIFQYGAGSNPGLMLGYNRLFSAYEKDFGKFTVVDAFFSVANGNTS
STFFSEGNEKDKYNRSFLPNMQISYWFKRCGELKDWFFRFHGEALDVNFVPSFMDVIE
STLQSMRAFQELKKNILDVSESLRAENDNSYASTSVESASSSLAPFLDNIRSVNSNFK
YDGGVFRVYTYEDIETKSEPSFEIKSPVVTINCTYKHDEDKVKPHKFRTLITVDPTHN
TLYAGCAPLLMEFSESLQKMIKKHSTDEKPNFTKPSSQNVDYKRLLDQFDVAVKLTSA
KQQLSLSCEPKAKVQADVGFESFLFSMATNEFDSEQPLEFSLTLEHTKASIKHIFSRE
VSTSFEVGFMDLTLLFTHPDVISMYGTGLVSDLSVFFNVKQLQNLYLFLDIWRFSSIL
HTRPVQRTVNKEIEMSSLTSTNYADAGTEIPWCFTLIFTNVSGDVDLGPSLGMISLRT
QRTWLATDHYNEKRQLLHAFTDGISLTSEGRLSGLFEVANASWLSEVKWPPEKSKNTH
PLVSTSLNIDDIAVKAAFDYHMFLIGTISNIHFHLHNEKDAKGVLPDLLQVSFSSDEI
ILSSTALVVANILDIYNTIVRMRQDNKISYMETLRDSNPGESRQPILYKDILRSLKLL
RTDLSVNISSSKVQISPISLFDVEVLVIRIEKVSIRSETHSGKKLKTDLQLQVLDVSA
ALSTSKEELDEEVGASIAIDDYMHYASKIVGGTIIDIPKLAVHMTTLQEEKTNNLEYL
FACSFSDKISVRWNLGPVDFIKEMWTTHVKALAVRRSQVANISFGQTEEELEESIKKE
EAASKFNYIALEEPQIEVPQIRDLGDATPPMEWFGVNRKKFPKFTHQTAVIPVQKLVY
LAEKQYVKILDDTH"
gene 294783..296626
/gene="Gaa1"
/locus_tag="R008_L10946"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(296912..298690)
/gene="Alt1"
/locus_tag="R008_L10951"
mRNA complement(296912..298690)
/gene="Alt1"
/locus_tag="R008_L10951"
/product="Alt1p"
CDS complement(296912..298690)
/gene="Alt1"
/locus_tag="R008_L10951"
/note="Similar to YLR089C 'Alanine transaminase (glutamic
pyruvic transaminase); involved in alanine biosynthetic
and catabolic processes; the authentic, non-tagged protein
is detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Alt1p"
/protein_id="EWG84359.1"
/translation="MLSLSAKNHFTVSNSITHVIKSYHIRTLTSSAEKMPHITTPFST
SASSTKLKAFRKVRPVLQRHSSSWIVAQNHRRSLSGQSSLNDLRHLNRFPHHTLKTSN
NEFYPAEQLTLEDVNENVLKAKYAVRGAIPMRAEELKAQLEKDPQSLPFDRIINANIG
NPQQLQQKPLTYYRQVLSLLQYPELLNQNEQQLVDSKLFKLDAIKRAKSLMEDIGGSV
GAYSSSQGVEGIRKSVAEFITKRDEGEISYPEDIFLTAGASAAVNYLLSIFCRGPETG
VLIPIPQYPLYTATLALNNSQALPYYLDENSGWSTNPEEIETVVKEAIQNEIKPTVLV
VINPGNPTGAVLSPESIAQIFEVAAKYGTVVIADEVYQENIFPGTKFHSMKKILRHLQ
REHPGKFDNVQLASLHSTSKGVSGECGQRGGYMELTGFSHEMRQVILKLASISLCPVV
TGQALVDLMVRPPVEGEESFESDQAERNSIHEKLITRAMTLYETFNSLEGIECQKPQG
AMYLFPKIDLPFKAVQEARHLELTPDEFYCKKLLESTGICTVPGSGFGQEPGTYHLRT
TFLAPGLEWIKKWESFHKEFFDQYRD"
gene 299376..300755
/gene="Xdj1"
/locus_tag="R008_L10956"
mRNA 299376..300755
/gene="Xdj1"
/locus_tag="R008_L10956"
/product="Xdj1p"
CDS 299376..300755
/gene="Xdj1"
/locus_tag="R008_L10956"
/note="Similar to YLR090W 'Putative chaperone, homolog of
E. coli DnaJ, closely related to Ydj1p; the authentic,
non-tagged protein is detected in highly purified
mitochondria in high-throughput studies'"
/codon_start=1
/product="Xdj1p"
/protein_id="EWG84360.1"
/translation="MSGSDRGDRLYDVLGVTRDATVQEIKTAYRKLALKHHPDKYVDQ
GSKEVNEIKFKEITAAYEILSDPEKKSHYDLYGDDNGAASSGGANGFGDEDFMNFFNN
FFNNGSHDGNNFPGEYDAYEEGNFTSSKDIDIDISLTLKDLYMGKKLKFDLKRQVICI
KCHGSGWKPKRKIHVTHDVECESCAGKGSKERLKRFGPGLVASQWVVCEKCNGKGKYT
KRPKNPKNFCPDCAGLGLLSKKEIITVNVAPGHHFDDVITVKGMADEEIDKTTCGDLK
FHLTEKQENLEQKQIFLKNFDDGAGEDLYTSITISLSEALTGFEKFLTKTFDDRLLTL
SVKPGRVVRPGDTIKIANEGWPILDNPHGRCGDLYVFVHIEFPPDNWFNEKSELLAIK
TNLPSSSSCASHATVNTEDDSNLTNNETISNFRIIHTDDLPEGIRPFKPEAQDSAYQK
ARSSYCCIQ"
gene 300969..301850
/locus_tag="R008_L10966"
mRNA 300969..301850
/locus_tag="R008_L10966"
/product="hypothetical protein"
CDS 300969..301850
/locus_tag="R008_L10966"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84361.1"
/translation="MASQVNALLLPVIESTPLHQITKVALTTTLTSKQSDYKFKEIAV
PLTKSLQLYEKAQRRQDLRASLKALESIIYQTHFQWNNPLPRHAHLFQKHYHFLLTHW
PFENHRDLVDSIAVNNGKLNSTSSRSVWLKADWITLFNVKNPWVQTPPSLMRLSGTDL
DTFTPERIFLINSLGNHYKFLIANSHLSYNHKKYPSPGVQIPIRNALGEVSPAKQIAQ
LFARQLSHIYKSLFIENPPLSPENELALTAVFYDETVERRLRRLYMRACARAYTTTNA
DSTTEPLMFHCTRWEVD"
gene 302216..304898
/gene="Sul2"
/locus_tag="R008_L10976"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(305185..306097)
/gene="Nyv1"
/locus_tag="R008_L10981"
mRNA complement(join(305185..305930,306082..306097))
/gene="Nyv1"
/locus_tag="R008_L10981"
/product="Nyv1p"
CDS complement(join(305185..305930,306082..306097))
/gene="Nyv1"
/locus_tag="R008_L10981"
/note="Similar to YLR093C 'v-SNARE component of the
vacuolar SNARE complex involved in vesicle fusion;
inhibits ATP-dependent Ca(2+) transport activity of Pmc1p
in the vacuolar membrane'"
/codon_start=1
/product="Nyv1p"
/protein_id="EWG84362.1"
/translation="MKRFNVSYVEVIKNGETISSCFQPFQKNENYGTITSANEQITPV
IFHNLIMDMVLPKVVPIKGNKVTKMSMNLIDGFDCFYSTDDHDPKTVYVCFTLVDIPK
ILPIRILSGLQEYESNATNELLSSHVGQILDSFHEELVEYRNQTLNSSGNGQSSNGNG
QNTISDIGDATEDQIKDVIQIMNDNIDKFLERQERVSLLVDKTSQLNSSSNKFRRKAV
NIKEIMWWQKVKNITLLTFTIILFVSAAFMFFYLW"
gene complement(306429..307937)
/gene="Gis3"
/locus_tag="R008_L10986"
mRNA complement(306429..307937)
/gene="Gis3"
/locus_tag="R008_L10986"
/product="Gis3p"
CDS complement(306429..307937)
/gene="Gis3"
/locus_tag="R008_L10986"
/note="Similar to YLR094C Protein of unknown function"
/codon_start=1
/product="Gis3p"
/protein_id="EWG84363.1"
/translation="MLLDVNTNHTLMHDAHVHEHCLIKSIRDDGALHSWSDSSKVFYP
KSFYATATNKKNNKLASASMNKTATSNRTVSDEIYFHSTEPQFDGQGSAERTRTLTKR
NSFKRTRILKARDDSELLNENRSSLMTPSLSSVMSQVRKTNSAKTLSGECPIHEGHLT
QSIKRKFSEEAQSDCSSLSSSKLHPLTDDIADAVDLQTPAISDEVLAEPVVPKMKIIN
INDLDLFDDWEVKDLVDIFPPVYERRPRSSSALSLVSASSDAKLRPTSVDFQIIDKKG
GKTSRRKSRSKSTTENMIYENDLVELEQWPSASPSPETDGSIASSELLPNKRIRQKSL
NTNFLKLYSIETSCKRKSILPEVEVDDHLLKQLTYSEIRSLEIKKEPNVSTNDIKLAL
ITRKKLWSDMVHETRNDLFGDSTPWNLHFVATTSNTEPSQGRESASEHATADLKSSLV
RVHSDVKPWFNNGGTMLKPCGKLNLGKVTNKTSAPTREIQYVVKGWCDSRFL"
gene complement(308376..310820)
/gene="Ioc2"
/locus_tag="R008_L10991"
mRNA complement(308376..310820)
/gene="Ioc2"
/locus_tag="R008_L10991"
/product="Ioc2p"
CDS complement(308376..310820)
/gene="Ioc2"
/locus_tag="R008_L10991"
/note="Similar to YLR095C Member of a complex (Isw1b) with
Isw1p and Ioc4p that exhibits nucleosome-stimulated ATPase
activity and acts within coding regions to coordinate
transcription elongation with termination and processing,
contains a PHD finger motif"
/codon_start=1
/product="Ioc2p"
/protein_id="EWG84364.1"
/translation="MRTKRTRSTRNVGASMPAGAANADSEDWKEYVSEDIIAQLNKHQ
LPYSEILDEKIADLATHWHFQYVMAWLSNVCESYTTTTFNTDQYGGSSTKCLWKNIKF
DEGVFVTDVFSKIDGKDSNYYNDEVDVDEGSQNLYDRIRLQLLHQLAGNKSGQLKDWN
VIVNHHLQNSSAYSDLVTDLPFLELEIARQFDIIYSIIKLIEMKNMIFKNYLANNLHL
FTFSEVILDDDNSGGEEMKSLFSLPNVGVLVKKTIHRVKEGSSSQVSQTLNIPIKLQN
CTIKESDPDIPDSVELIHLEYSHDIDAYLQSITIDYDVITTNWGSMLEYWSENKSSKA
IDEFITNLIPVYAEHRLYSAKLLTHREKERAIAELMTRRKRSSRLVAKEEENKKKDLE
SEWFEKLDEREQFIRHRNKLVSKEIKKIKDLLWNQLWQLYDQDYRDEKLTRRNELKDR
SGSGTPFFETSLGREEDNPLNEIDIGVLDHGPNFQSSIIPVEPPIPGTVGPLQTGDVP
ELPSDFCIIKEELDELANYGIFTPQQEPDNQDSVFQCPGQPELAPMIITEDTETDLFN
NRPLICCDHCYRWQHWECQPPKIIELISSTTKSPQHTLSQRDFGVIIMGNSHGNRRSS
RRPQSTLEPSTKSSRPTDKRKPLSECSTFICAWCIRDLELELRNIFVPELKIIRAKQR
KQQEDRERRKKMKEEKKRLEELAKKRELTESVSPPVFNNAFANMSSSTTPSIAAYEKT
NPAINPAPNVNAAHPIITYSQQTGSKTVPQAPQAPQTSQASIQPQQQQQQQQQQQQQP
LHPKEQNFHFQFPPTN"
gene 311294..314737
/gene="Kin2"
/locus_tag="R008_L11001"
mRNA 311294..314737
/gene="Kin2"
/locus_tag="R008_L11001"
/product="Kin2p"
CDS 311294..314737
/gene="Kin2"
/locus_tag="R008_L11001"
/note="Similar to YLR096W 'Serine/threonine protein kinase
involved in regulation of exocytosis; localizes to the
cytoplasmic face of the plasma membrane; closely related
to Kin1p'"
/codon_start=1
/product="Kin2p"
/protein_id="EWG84365.1"
/translation="MPNPNTADYLVNPNFRTSKGGSLSPTPEAFNDTRVAAPATLRMM
GKQSGPRNDQQQAPLMPPADIKQGKEQAAQRQNDASRPNGAVELRQFHRRSLGDWEFL
ETVGAGSMGKVKLVKHRQTKEICVIKIVNRASKAYLHKQHSLPSPKNESEILERQKRL
EKEIARDKRTVREASLGQVLYHPHICRLFEMCTMSNHFYMLFEYVSGGQLLDYIIQHG
SLKEHHARKFARGIASALQYLHANNIVHRDLKIENIMISSSGEIKIIDFGLSNIFDYR
KQLHTFCGSLYFAAPELLKAQPYTGPEVDIWSFGIVLYVLVCGKVPFDDENSSILHEK
IKKGKVDYPSHLSIEVISLLTRMIVVDPLRRATLKNVVEHPWMNRGYDFKAPSYVPNR
VPLTPEMIDSQVLKEMYRLEFIDDIEDTRRSLIRLVTEKEYIQLSQEYWDKLSNAKGL
SSSLNNNYLNSTAQQTLIQNHITSNPSQSGYNEPDSNFEDPTLAYHPLLSIYHLVSEM
VARKLAKLQRRQALALQAQAQQRQQQQQVALGTKVALNNNSPDIMTKMRSPQKEVVPN
PGIFQVPAIGTSGTSNNTNTSNKPPLHVMVPPKLTIPEQAHTSPTSRKSSDIHTELNG
VLKSTPVPVSGEYQQRSASPVVGEHQEKNTIGGIFRRISQSGQSQHPTRQQEPLPERE
PPTYMSKSNEISIKVPKSHSRTISDYIPSARRYPSYVPNSVDVKQKPAKNTTIAPPIR
SVSQKQNSDLPALPQNAELIVQKQRQKLLQENLDKLQINDNDNNNVNAVVDGINNDNS
DHYLSVPKGRKLHPSARAKSVGHARRESLKFTRPPIPAALPPSDMTNDNGFLGEANKE
RYNPVSSNFSTVPEDSTTYSNDTNNRLTSVYSQELTEKQILEEASKAPPGSMPSIDYP
KSMFLKGFFSVQTTSSKPLPIVRHNIISVLTRMNIDFKEVKGGFICVQQRPSIETAAV
PVITTTGVGLDSGKAMDLQNSLDSQLSSSYHSTASSASRNSSIKRQGSYKRGQNNIPL
TPLATNTHQRNSSIPMSPNYGNQSNGTSGELSSMSLDYVQQQDDILTTSRAQNINNVN
GQTEQTNTSGIKERPPIKFEIHIVKVRIVGLAGVHFKKVSGNTWLYKELASYILKELN
L"
gene complement(314935..315969)
/gene="Hrt3"
/locus_tag="R008_L11006"
mRNA complement(314935..315969)
/gene="Hrt3"
/locus_tag="R008_L11006"
/product="Hrt3p"
CDS complement(314935..315969)
/gene="Hrt3"
/locus_tag="R008_L11006"
/note="Similar to YLR097C 'Putative SCF-ubiquitin ligase
F-box protein, based on both genetic and physical
interactions and sequence similarity; identified in
association with Cdc53p, Skp1p and Ubi4 in large and
small-scale studies'"
/codon_start=1
/product="Hrt3p"
/protein_id="EWG84366.1"
/translation="MIVDYEKDPRAKEAIAIWEKGVLKEKDGSMSDAINFYRSALKIH
DNVESLYRKKILDEWMLHKKLSGLSMTTDAPDEQNETGKDDLSAEENAELQPCWILEI
LPDDILLRIIKKVILMSGESWVNLSMTCSTFSKLCFHDSVPFKTFAKYIYSKQIYDKM
AMDLNGITDINTFEKEIWRGDDYRMLRERPYIKFEGVYISVVNYVRYGSNAESSLSLL
KPVHMITYYRYFRFYENGQCLRLLSTDEPSAVVKHFSKENKPRHSHMCYWSLGFDYDF
GHLKITRSDEKYTFIEEFQIKNQGNKRYQRLKWLSSIVVDKEGNASNCSLRNEKSFFF
SRVKSFKDPG"
gene complement(318438..319622)
/gene="Ict1"
/locus_tag="R008_L11021"
mRNA complement(318438..319622)
/gene="Ict1"
/locus_tag="R008_L11021"
/product="Ict1p"
CDS complement(318438..319622)
/gene="Ict1"
/locus_tag="R008_L11021"
/note="Similar to YLR099C 'Lysophosphatidic acid
acyltransferase, responsible for enhanced phospholipid
synthesis during organic solvent stress; null displays
increased sensitivity to Calcofluor white; highly
expressed during organic solvent stress'"
/codon_start=1
/product="Ict1p"
/protein_id="EWG84367.1"
/translation="MWTNTFKWCSKTEKETTTADAKVCASVQGLKALQQQIMDSTTVR
GSVNNTMTPGGINQWHFHNKRANKVCTPTVLIHGYAASSMAFYRTFENLSDNIKDLYA
IDLPANGASEAPALQVNKTKKIKSLRFKHIEDDVVIPVIEKRPPAEDIKSHLEQYESY
FVDRIEQWRKDNKLRKINVVGHSFGGYISFKYALKYPDSIEKLCLISPLGVENSIHAI
THKWEPNTTYPLTFTDPSSRYYTRKLNVPRFIFENQLNVLKWMGPIGSKLCSNYISTA
YVKVPDQIYKDYLLHSFVGKNQTVQPQTIKVFTHLFERNLIARDPIINNVRFLNPATP
VMFMYGEHDWMDKYAGYLTTESMLKNKAKASYIEVPDAGHNLFLDNPQHFASSLVSFL
SK"
gene 320019..320282
/locus_tag="R008_L11026"
mRNA 320019..320282
/locus_tag="R008_L11026"
/product="hypothetical protein"
CDS 320019..320282
/locus_tag="R008_L11026"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84368.1"
/translation="MADSEDTSVILQGIDTINSVEGLEEDGYLSDEDTSLSNELADAQ
RQWEESLQQLNKLLNWVLLPLLGKYIGRRMAKTLWSRFIEHFV"
gene 320504..321547
/gene="Erg27"
/locus_tag="R008_L11031"
mRNA 320504..321547
/gene="Erg27"
/locus_tag="R008_L11031"
/product="Erg27p"
CDS 320504..321547
/gene="Erg27"
/locus_tag="R008_L11031"
/note="Similar to YLR100W '3-keto sterol reductase,
catalyzes the last of three steps required to remove two
C-4 methyl groups from an intermediate in ergosterol
biosynthesis; mutants are sterol auxotrophs'"
/codon_start=1
/product="Erg27p"
/protein_id="EWG84369.1"
/translation="MNRKVAIVTGTNSNLGLNIVFRLIETEDTNVRLTIVVTSRTLPR
VQEVINQIKDFYNKSGRVEDLEIDFDYLLVDFTNMVSVLNAYYDINKKYRAINYLFVN
AAQGIFDGIDWIGAVKEVFTNPLEAVTNPTYKIQLVGVKSKDDMGLIFQANVFGPYYF
ISKILPQLTRGKAYIVWISSIMSDPKYLSLNDIELLKTNASYEGSKRLVDLLHLATYK
DLKKLGINQYVVQPGIFTSHSFSKYLNFFTYFGMLCLFYLARLLGSPWHNIDGYKAAN
APVYVTRLANPNFEKQDVKYGSATSRDGMPYIKTQEIDPTGMSDVFAYIQKKKLEWDE
KLKDQIVETRTPI"
gene complement(321664..322461)
/gene="Apc9"
/locus_tag="R008_L11041"
mRNA complement(321664..322461)
/gene="Apc9"
/locus_tag="R008_L11041"
/product="Apc9p"
CDS complement(321664..322461)
/gene="Apc9"
/locus_tag="R008_L11041"
/note="Similar to YLR102C Subunit of the
Anaphase-Promoting Complex/Cyclosome (APC/C), which is a
ubiquitin-protein ligase required for degradation of
anaphase inhibitors, including mitotic cyclins, during the
metaphase/anaphase transition"
/codon_start=1
/product="Apc9p"
/protein_id="EWG84370.1"
/translation="MNQNGDKNEGKLFQLPSLPPWKTPRFNKANFNNFTTPLRKRSTR
VINDDSMPITGEVLEERTADDLYGINMDVDEVDYLNTLSHIEEEKQYDYSPFCERNTL
RESRIDSFLKAERAAHCLVFHKVGHLDGIDSYRPDIDIMCGEEANKYDSANPEGNGSM
LLESVPGCNKEDLERLSRREFVTNSKPNMRRLDDIINHETNALKSFWNDSGLVNSLQS
HHLHEEYLLLQEELKNVYKIKCHDRVPIESLRDKCRRHYSNEDSSFL"
assembly_gap 322631..322659
/estimated_length=29
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(322693..324654)
/gene="Cdc45"
/locus_tag="R008_L11046"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 325298..325693
/locus_tag="R008_L11051"
mRNA 325298..325693
/locus_tag="R008_L11051"
/product="hypothetical protein"
CDS 325298..325693
/locus_tag="R008_L11051"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84371.1"
/translation="MSQSRWSIVLIFALFIFGSTGVNAFFNFGHHQQQQQQQQQSYED
QVLNNPCDGYLCPDTLTCVAQQKDCPCPFPKSQLKCVLPDNKFVCISKPATHNEKFRA
IYDDPVKGPKAKNKGFRDCGWVSDAYKNH"
gene complement(325760..326893)
/gene="Sen2"
/locus_tag="R008_L11056"
mRNA complement(325760..326893)
/gene="Sen2"
/locus_tag="R008_L11056"
/product="Sen2p"
CDS complement(325760..326893)
/gene="Sen2"
/locus_tag="R008_L11056"
/note="Similar to YLR105C 'Subunit of the tRNA splicing
endonuclease, which is composed of Sen2p, Sen15p, Sen34p,
and Sen54p; Sen2p contains the active site for tRNA 5'
splice site cleavage and has similarity to Sen34p and to
Archaeal tRNA splicing endonuclease'"
/codon_start=1
/product="Sen2p"
/protein_id="EWG84372.1"
/translation="MSKGRVNQKRYKYPLPIHPVDDLPELILHNPLSWLYWAYRYYKS
TNALNDKVHVDFIGDTTLHITVQDDKQMLYLWNNGFFGTGQFSRSEPTWKARTEARLG
LNDTPLHNRGGTKSNTETEMTLEKVTQQRRLQRLEFKKERAKLERELLELRKKGGHID
EENILLEKQRESLRKFKLKQTEDVGIVAQQQDISESNLRDEDNNLLDENGDLLPLESL
ELMPVEAMFLTFALPVLDISPACLAGKLFQFDAKYKDIHSFVRSYVIYHHYRSHGWCV
RSGIKFGCDYLLYKRGPPFQHAEFCVMGLDHDVSKDYTWYSSIARVVGGAKKTFVLCY
VERLISEQEAIALWKSNNFTKLFNSFQVGEVLYKRWVPGRNRD"
gene complement(327150..327233)
/locus_tag="R008_L11066"
ncRNA complement(327150..327233)
/ncRNA_class="snoRNA"
/locus_tag="R008_L11066"
/product="SNR79"
gene complement(327740..342471)
/gene="Mdn1"
/locus_tag="R008_L11071"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 342849..344063
/gene="Rex3"
/locus_tag="R008_L11076"
mRNA 342849..344063
/gene="Rex3"
/locus_tag="R008_L11076"
/product="Rex3p"
CDS 342849..344063
/gene="Rex3"
/locus_tag="R008_L11076"
/note="Similar to YLR107W 'RNA exonuclease; required for
maturation of the RNA component of RNase MRP; functions
redundantly with Rnh70p and Rex2p in processing of U5
snRNA and RNase P RNA; member of RNase D family of
exonucleases'"
/codon_start=1
/product="Rex3p"
/protein_id="EWG84373.1"
/translation="MGSLLRPVDLVNQPLGFQERYKILQKLFKQLQKAYAHTKGTNID
LERLATRLEVHVAKNSLSGQSYKFNMSILLRDVLKYKGDLSKIKINGRPLKGAKPHLS
SIGNANSITTKSKAMEALKALILDSKVLEKNGYIVKEMQNKTNDDNSTQLYAPCLRCS
SNFKKTDIMEKTLCRYHPLKRIYNRDTKNHQYPCCGETTDSVSFLRLGCKTFFHHVFR
GESYDELCKISKFSSTDDIDGVENVLSLDCEMAFTSLGYEMIRLTIVDFFTGKTLFDH
VIQPIGDIVDLNSDFSGVHEIDRTNCPTYKEALDVFLSENLINKNSILIGHGLENDLN
VMRLFHNKVIDTAILYSKTKFKVSLKNLAFEVLSRKIQNGEHDSSQDAIATMDVVKVK
IGISPSQNKWEK"
gene 344968..345079
/locus_tag="R008_L11081"
ncRNA 344968..345079
/ncRNA_class="snoRNA"
/locus_tag="R008_L11081"
/product="SNR6"
gene complement(345400..346857)
/locus_tag="R008_L11086"
mRNA complement(345400..346857)
/locus_tag="R008_L11086"
/product="hypothetical protein"
CDS complement(345400..346857)
/locus_tag="R008_L11086"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84374.1"
/translation="MSGQKGEIVVYTKELETTPELLPNHEVFKIRIGQKLFEISGATL
NSDAPNFFTQFFNTHDKNTILFVDRSEDVFIIIYRHLQGYFPDIKNEVQFTCLFADAL
YFQLPKLVKLIKEYDYHFTNIGGVPFKVPKSLFHEEGNRLNYFETISRISYEEIEKWE
SNKKPGFPPLLPPSYIARSPEFFRDILSLLGGSKLELSEERTASLIKECRYYRLNRLE
QELVRAKIIYNPLTNCQEVCIALDSVSKKGVTIERLTSLHTGNQSLAVSSCLNGTEGE
KAATGFHKTETDSGNNDEYEPPTKKVKHCIERHWSMLKYQRPYIDTVSHDLIFQLHSN
QCKIIFNKKNKTVHVDLSREAAMLFENKFSDVLLENPDFKIDLSEYKVKLRDSQMQVE
SHLIIPACVSICDLTVNGAKCCNIFSLVNDSKCKGRVLDCTNLKVLNCVHGLKLHLSK
SMWKLGTNNGRIILVAVKAETFSGTKEYCKMIDFL"
gene 347514..348044
/gene="Ahp1"
/locus_tag="R008_L11091"
mRNA 347514..348044
/gene="Ahp1"
/locus_tag="R008_L11091"
/product="Ahp1p"
CDS 347514..348044
/gene="Ahp1"
/locus_tag="R008_L11091"
/note="Similar to YLR109W 'Thiol-specific peroxiredoxin,
reduces hydroperoxides to protect against oxidative
damage; function in vivo requires covalent conjugation to
Urm1p'"
/codon_start=1
/product="Ahp1p"
/protein_id="EWG84375.1"
/translation="MSDLVNKKFPAGDYKFQYIAISQSDADSESCKMPQTVEWSKLIS
ENKKVIITGAPAAFSPTCTVSHIPGYINYLDELVKEKEVDQVIVVTVDNPFANQAWAK
SLGVKDTTHIKFASDPGCAFTKSIGFELAVGDGVYWSGRWAMVVENGIVTYAAKETNP
GTDVTVSSVESVLAHL"
gene complement(348432..348833)
/gene="Ccw12"
/locus_tag="R008_L11096"
mRNA complement(348432..348833)
/gene="Ccw12"
/locus_tag="R008_L11096"
/product="Ccw12p"
CDS complement(348432..348833)
/gene="Ccw12"
/locus_tag="R008_L11096"
/note="Similar to YLR110C 'Cell wall mannoprotein with a
role in maintenance of newly synthesized areas of cell
wall; localizes to periphery of small buds, septum region
of larger buds, and shmoo tip'"
/codon_start=1
/product="Ccw12p"
/protein_id="EWG84376.1"
/translation="MQFSTVASIAAVAAVASAAANVTTATVSQESTTLVTITSCEDHV
CSETVSPALVSTATVTVDDVITQYTTWCPLTAEAPKNGTSTAAPVTSTEAPKNTTSAA
PTHSVTSYTGAAAKALPAAGALLAGAAALLL"
gene 350351..351658
/gene="Hog1"
/locus_tag="R008_L11116"
mRNA 350351..351658
/gene="Hog1"
/locus_tag="R008_L11116"
/product="Hog1p"
CDS 350351..351658
/gene="Hog1"
/locus_tag="R008_L11116"
/note="Similar to YLR113W 'Mitogen-activated protein
kinase involved in osmoregulation; acts via three
independent osmosensors; mitophagy-specific regulator;
mediates the recruitment and activation of RNA Pol II at
Hot1p-dependent promoters; localization regulated by Ptp2p
and Ptp3p'"
/codon_start=1
/product="Hog1p"
/protein_id="EWG84377.1"
/translation="MTTNEEFIRTQIFGTVFEITNRYNDLNPVGMGAFGLVCSATDTL
TSQPVAIKKIMKPFSTAVLAKRTYRELKLLKHLRHENLICLQDIFLSPLEDIYFVTEL
QGTDLHRLLQTRPLEKQFVQYFLYQILRGLKYVHSAGVIHRDLKPSNILINENCDLKI
CDFGLARIQDPQMTGYVSTRYYRAPEIMLTWQKYDVEVDIWSAGCIFAEMIEGKPLFP
GKDHVHQFSIITDLLGSPPKDVINTICSENTLKFVTSLPHRDPIPFSERFKTVEPDAV
DLLEKMLVFDPKKRITAADALAHPYSAPYHDPTDEPVADAKFDWHFNDADLPVDTWRV
MMYSEILDFHKIGGSDGQIDISATFDDQVAAATAAAAQAQAQAQAQVQLNMAAHSHNG
AGTTGNDHSDIAGGNKVSDHVAANDTITDYGNQAIQYANEFQQ"
gene 353094..353175
/locus_tag="R008_L11121"
tRNA 353094..353175
/locus_tag="R008_L11121"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg2);
TR(ACG)L"
gene complement(353693..355986)
/gene="Avl9"
/locus_tag="R008_L11126"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 356733..359312
/gene="Cft2"
/locus_tag="R008_L11131"
mRNA 356733..359312
/gene="Cft2"
/locus_tag="R008_L11131"
/product="Cft2p"
CDS 356733..359312
/gene="Cft2"
/locus_tag="R008_L11131"
/note="Similar to YLR115W 'Subunit of the mRNA cleavage
and polyadenlylation factor (CPF); required for pre-mRNA
cleavage, polyadenylation and poly(A) site recognition'"
/codon_start=1
/product="Cft2p"
/protein_id="EWG84378.1"
/translation="MTYKYNCCDDGSGTTVGSVVRFDNVTLLIDPGWNPSKVSYEQCI
KYWEKVIPEIDVIILSQPTIECLGAHSLLYYNFTSHFISRIQVYATLPVINLGRVSTI
DSYASAGVIGPYDTNKLDLEDIEISFDHIVPLKYSQLVDLRSRYDGLTLLAYNAGVCP
GGSIWCISTYSEKLVYAKRWNHTRDNILNAASILDATGKPLSTLMRPSAIITTLDRFG
SSQPFKKRSKIFKDTLKKGLSSDGSVIIPVDMSGKFLDLFTQVHELLFESTKINAHTQ
VPVLILSYARGRTLTYAKSMLEWLSPSLLKTWENRNNTSPFEIGSRIKIIAPNELSKY
PGSKICFVSEVGALINEVIIKVGNSEKTTLILTKPSFECASSLDKILEIVEQGERNWK
TFPEDGKSFLCDNYISIDTIKEEPLSKEETEAFKVQLKEKKRERNKKILLVKRESKKL
ANGNAIIDDTNGERAMRNQDILVENVNGVPPIDHIMGGDEDDDEEEENDNLLNLLKDN
SEKSAAKKNTEVPVDIIIQPSAASKHKMFPFNPAKIKKDDYGTVVDFTMFLPDDSDNV
NQNSRKRPLKDGAKTTSPVNEEDNKNEEEDGYNMSDPVSERSKHRASRYSGFSGTGEA
ENFDNLDYLKIDKTLSKRTTSTVNVQLKCSVVILNLQSLVDQRSASIIWPSLKSRKIV
LSAPKQIQNEEITAKLIKKNIEVVNMPLNKIVEFSTTIKTLDISIDSNLDNLLKWQRI
SDSYTVATVVGRLVKESLPQVNNHQKTASRSKLVLKPLHGSSRSHKTGALSIGDVRLA
QLKKLLTEKNYIAEFKGEGTLVINEKVAVRKINDAETIIDGTPSELFDTVKKLVTDML
AKI"
gene 359570..361000
/gene="Msl5"
/locus_tag="R008_L11136"
mRNA 359570..361000
/gene="Msl5"
/locus_tag="R008_L11136"
/product="Msl5p"
CDS 359570..361000
/gene="Msl5"
/locus_tag="R008_L11136"
/note="Similar to YLR116W 'Component of the commitment
complex, which defines the first step in the splicing
pathway; essential protein that interacts with Mud2p and
Prp40p, forming a bridge between the intron ends; also
involved in nuclear retention of pre-mRNA'"
/codon_start=1
/product="Msl5p"
/protein_id="EWG84379.1"
/translation="MSFRRINSRYFENRKGSSMEEKKAKVPPNVNLSLWRKNTVESDV
HRFNSLPSKISGALTREQIYSYQVMFRIQEITIKLRTNDFVPPSRKNRSPSPPPVYDA
QGKRTNTREQRYRKKLEDERIKLVEIALKTIPYFVPPDDYKRPTKFQDKYYIPVDQYP
DVNFVGLLLGPRGRTLRKLQEDSNCKIAIRGRGSVKEGKNASDLPPGAMNFEDPLHCL
IIADSEDKIQKGIKVCQNIVIKAVTSPEGQNDLKRGQLRELAELNGTLREDNRPCPIC
GLKDHKRYDCPNRKIPNIQGIVCKICGQTGHFSRDCNSSSQRMSRFDRNATVNNSAPI
QSNDVHYNSNTHPIQAPKRSRYDNNSTEPPLKFPASSRYAPSPSPPASHISRQAQNVT
PTPPPGLTSSSFSSGVPGIAPPPLQSPPESEQPKFSLPPPPGMTTVQSSIAPPPGLSG
PPGFSNNMGNDINKPTPPGLQGPPGL"
gene complement(361219..363282)
/gene="Clf1"
/locus_tag="R008_L11141"
mRNA complement(361219..363282)
/gene="Clf1"
/locus_tag="R008_L11141"
/product="Clf1p"
CDS complement(361219..363282)
/gene="Clf1"
/locus_tag="R008_L11141"
/note="Similar to YLR117C 'Member of the NineTeen Complex
(NTC) that contains Prp19p and stabilizes U6 snRNA in
catalytic forms of the spliceosome containing U2, U5, and
U6 snRNAs; homolog of Drosophila crooked neck protein;
interacts with U1 snRNP proteins'"
/codon_start=1
/product="Clf1p"
/protein_id="EWG84380.1"
/translation="MDTLEPTAVDTHVSAEQILRDVYKKGQKARGSTNIDILDLEELR
EYQRRKRTEYEGYLKRNRLDMGQWIRYAQFEIEQHDMRRARSIFERALLVDSSFIPLW
IRYIDAELKVKCINHARNLMNRAISTLPRVDKLWYKYLIVEESLNNVEIVRSLYTKWC
SLEPGVNAWNSFVDFEIRQKNWNGVREIYSKYVMAHPQMQTWLKWVRFENRHGNTEFT
RSVYSLAIDTVANLQNLQVWSDMEVAKLVNSFAHWEAAQQEYERSSALYQIAIEKWPS
NQLLKAGLLDFEKQFGDINSIEETISYKRKMEYETILSNNAYDYDTWWLYLDLISESF
PKQIMQTFEKAIVDSRPKELSKNVQWKRYIYLWMRYICYVELELENSLLEEELFQRLI
DDIIPHKHFTFSKIWLMYAKFLIRHDDVPKARKILGKAIGLCPKAKTFKGYIELEVKL
KEFDRVRKIYEKFIEFQPSDLQIWSQYGELEENLGDWDRVRGIYTIALDENSDFLTKE
AKIVLLQKYITFETESQEFEKARKLYRRYLELNQYSPQSWIEFAMYQTSTPTEQQLLD
LAKLQSENVDEDIEFEITDENKLEARKVFEEAIVFFKEKDDKQGRLSILEALKDYEET
YGTELDQETVKKRFPKVIKKVRLQDGVEEEFVDYVFPDDIDDDKPKPSKFLELAKKWK
QEQAL"
gene complement(363473..364156)
/locus_tag="R008_L11146"
mRNA complement(363473..364156)
/locus_tag="R008_L11146"
/product="hypothetical protein"
CDS complement(363473..364156)
/locus_tag="R008_L11146"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84381.1"
/translation="MNGLRVAAKIQPARQTIIFLHGLGDTGSGWGFLAQYLQQRDPAA
FQHTNFVFPNAPELHVTANGGALMPAWFDILEWDPSFSKVDSDGFMNSLNSIEKTVKQ
EIDKGIKPEQIIIGGFSQGAALALATSVTLPWKIGGIVALSGFCSIPGILKQHKNGIN
VKTPIFHGHGDMDPVVPIGLGIKAKQFYQDSCEIQNYEFKVYKGMAHSTVPDELEDLA
SFIKKSLSS"
gene 364282..364921
/gene="Srn2"
/locus_tag="R008_L11151"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(365257..366966)
/gene="Yps1"
/locus_tag="R008_L11156"
mRNA complement(365257..366966)
/gene="Yps1"
/locus_tag="R008_L11156"
/product="Yps1p"
CDS complement(365257..366966)
/gene="Yps1"
/locus_tag="R008_L11156"
/note="Similar to YLR120C 'Aspartic protease, member of
the yapsin family of proteases involved in cell wall
growth and maintenance; attached to the plasma membrane
via a glycosylphosphatidylinositol (GPI) anchor'"
/codon_start=1
/product="Yps1p"
/protein_id="EWG84382.1"
/translation="MKLKTVRSAVLSSLFASQVLGKIIPAANKRDDDSNSKFVKLPFH
KLYGDSLENVGSDKKPEVRLLKRADGYEEIIITNQQSFYSVDLEVGTPPQNVTVLVDT
GSSDLWIMGSDNPYCSSNSMGSSRRRVIDKRDDSSSGGSLINDINPFGWLTGTGSAIG
PTATGLGGGSGTATQSVPASEATMDCQQYGTFSTSGSSTFRSNNTYFSISYGDGTFAS
GTFGTDVLDLSDLNVTGLSFAVANETNSTMGVLGIGLPELEVTYSGSTASHSGKAYKY
DNFPIVLKNSGAIKSNTYSLYLNDSDAMHGTILFGAVDHSKYTGTLYTIPIVNTLSAS
GFSSPIQFDVTINGIGISDSGSSNKTLTTTKIPALLDSGTTLTYLPQTVVSMIATELG
AQYSSRIGYYVLDCPSDDSMEIVFDFGGFHINAPLSSFILSTGTTCLLGIIPTSDDTG
TILGDSFLTNAYVVYDLENLEISMAQARYNTTSENIEIITSSVPSAVKAPGYTNTWST
SASIVTGGNIFTVNSSQTASFSGNLTTSTASATSTSSKRNVGDHIVPSLPLTLISLLF
AFI"
gene 367417..367515
/locus_tag="R008_L11161"
mRNA 367417..367515
/locus_tag="R008_L11161"
/product="hypothetical protein"
CDS 367417..367515
/locus_tag="R008_L11161"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84383.1"
/translation="MLFLLKSRKEGKEKVSIYSVYCFFFTKRKRRG"
gene complement(367490..369017)
/gene="Yps3"
/locus_tag="R008_L11166"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 372231..372641
/locus_tag="R008_L11191"
mRNA 372231..372641
/locus_tag="R008_L11191"
/product="hypothetical protein"
CDS 372231..372641
/locus_tag="R008_L11191"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84384.1"
/translation="MGEILELTNKNFMSHLKKDITSQESLKSRIEDKNGDVASPKEDN
YPLLNETAAWPDGVITSEEGCSSSGEKENSGLCSEESSEEDPEEAEEESARAFGELVA
VLRDKDIPLNVLDEPQMKDWLEKYTGVYRSSWHG"
gene complement(373514..374269)
/locus_tag="R008_L11196"
mRNA complement(373514..374269)
/locus_tag="R008_L11196"
/product="hypothetical protein"
CDS complement(373514..374269)
/locus_tag="R008_L11196"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84385.1"
/translation="MTVKKIAILYTDEDNEWSKPWGNFVDMAIKLLEQTRKLECIAED
VEYEVFHVQKNVFPQLSDLQKDEYLGIYITGSKYDSFDNEIEWIMKLRSFLNEMLTSK
TEYPPVAGICFGHQVIAAALGSSVGRNPKGFEGGVVSLKLNSVGQKLFGAQELNLSEV
HSDCVFDVPEGYQNWASSEKCQNQGFYRQNRVLTFQGHPEFNSDVAQKGLLKSQDKLT
LEEFNRYERQCQELDNNGIQAARNIWRLFLQKI"
gene complement(374507..377067)
/gene="Apc2"
/locus_tag="R008_L11201"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 377278..378181
/gene="Dcn1"
/locus_tag="R008_L11211"
mRNA join(377278..377280,377375..378181)
/gene="Dcn1"
/locus_tag="R008_L11211"
/product="Dcn1p"
CDS join(377278..377280,377375..378181)
/gene="Dcn1"
/locus_tag="R008_L11211"
/note="Similar to YLR128W 'Scaffold-type E3 ligase
required for cullin neddylation and ubiquitin ligase
activation; contains a ubiquitin-binding domain (UBA) for
ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain
involved in cullin binding and neddylation'"
/codon_start=1
/product="Dcn1p"
/protein_id="EWG84386.1"
/translation="MSNNKIKRKDASPEQEAIESFTSLTKCDPKVSRKYLQRNHWNIN
YALNDYYDKEIGTFTDEVSTVAHPPVYPKELTQVFEHYINNNLFDIDSLVKFIEELGY
NLEDLATLCLAHLLGYKKLEEPLKREDFLSTWFMQGCSTISDMQECIKTLDVKLHEDL
QYFTQIYNYAFNLILDPNRKDIDTDEGIQYWKLFFQPEYPVRMEPDLLEAWFRFLRDE
GKTTISKDTWRMLLLFFKRYPTIQKIISDYDETAAWPFIIDEFYECLQDQQ"
gene 378405..381236
/gene="Dip2"
/locus_tag="R008_L11216"
mRNA 378405..381236
/gene="Dip2"
/locus_tag="R008_L11216"
/product="Dip2p"
CDS 378405..381236
/gene="Dip2"
/locus_tag="R008_L11216"
/note="Similar to YLR129W Nucleolar protein, specifically
associated with the U3 snoRNA, part of the large
ribonucleoprotein complex known as the small subunit (SSU)
processome, required for 18S rRNA biogenesis, part of the
active pre-rRNA processing complex"
/codon_start=1
/product="Dip2p"
/protein_id="EWG84387.1"
/translation="MVKSYQRFEQAAAFGVIASNANCVWIPASSGNSNGSGPGQLITS
ALEDVNIWDIKTGDLVSKLSDGLPPGASDARGAKPAECTYLEAHKDTDLLAVGYADGV
IKVWDLMSKTVLLNFNGHKAAITLLQFDGTGTRLISGSKDSNIIVWDLVGEVGLYKLR
SHKDSITGFWCQGEDWLISTSKDGMIKLWDLKTHQCIETHIAHTGECWGLAVKDDLLI
TTGTDSQVKIWKLDIENDKMGGKLTEMGIFEKQSKQRGLKIEFITNSSDKTSFFYIQN
ADKTIETFRIRKEEEIARGLKKREKRLKEKGLTEEEIAKSIKESYSSFILHPFQTIRS
LYKIKSASWTTVSSSKLELVLTTSSNTIEYYSIPYEKRDPTSPAPLKTHTIELQGQRT
DVRSIDISDDNKLLATASNGSLKIWNIKTHKCIRTFECGYALTCKFLPGGLLVILGTR
NGELQLFDLASSSLLDTIEDAHDAAIWSLDLTSDGKRLVTGSADKTVKFWDFKVENSL
VPGTKNKFLPVLKLHHDTTLELTDDILCVRVSPDDRYLAISLLDNTVKVFFLDSMKFY
LSLYGHKLPVLSIDISFDSKMIITSSADKNIKIWGLDFGDCHKSLFAHQDSIMNVKFL
PQSHNFFSCSKDAVVKYWDGEKFECIQKLYAHQSEVWALAVATDGGFVVSSSHDHSIR
IWEETEDQVFLEEEKEKELEEQYEDTLLTSLEEGNGDDAFKADASGEGVEDEASGVHK
QTLESLKAGERLMEALDLGIAEIEGLEAYNRDMKLWQRKKLGEAPIKPQGNAVLIAVN
KTPEQYIMDTLLRIRMSQLEDALMVMPFSYVLKFLKFIDTVMQNKTLLHSHLPLICKN
LFFIIKFNHKELVSQKNEELKLQINRVKTELRSALKSTEDDLGFNVQGLKFVKQQWNL
RHNYEFVDEYDQQEKESNSARKRVFGTVI"
gene complement(381540..382808)
/gene="Zrt2"
/locus_tag="R008_L11221"
mRNA complement(381540..382808)
/gene="Zrt2"
/locus_tag="R008_L11221"
/product="Zrt2p"
CDS complement(381540..382808)
/gene="Zrt2"
/locus_tag="R008_L11221"
/note="Similar to YLR130C 'Low-affinity zinc transporter
of the plasma membrane; transcription is induced under
low-zinc conditions by the Zap1p transcription factor'"
/codon_start=1
/product="Zrt2p"
/protein_id="EWG84388.1"
/translation="MVDLIARDDSVDTCQASNGYNGHAGLRILAVFIILISSGLGVYF
PILSSRYSFIRLPNWCFFIAKFFGSGVIVATAFVHLLQPAAEALGDECLGGTFAEYPW
AFGICLMSLFLLFFTEIITHYFVAKTLGHDHGDHGEVTSIDVDAPSSGFVIRNMDSDP
VSFNNEAAYSIHNDKTPYTTRNEEIVATPIKEKEPGSNVTNYDLEPGKTESLANELVP
TSSHATNLASVPGKDHYSHENDHQDVSQLATRIEEEDKEQYLNQILAVFILEFGIIFH
SVFVGLSLSVAGEEFETLFIVLTFHQMFEGLGLGTRVAETNWPESKKYMPWLMGLAFT
LTSPIAVAVGIGVRHSWIPGSRRALIANGVFDSISSGILIYTGLVELMAHEFLYSNQF
KGPDGLKKMLSAYLIMCCGAALMALLGKWA"
gene complement(383256..385568)
/gene="Ace2"
/locus_tag="R008_L11226"
mRNA complement(383256..385568)
/gene="Ace2"
/locus_tag="R008_L11226"
/product="Ace2p"
CDS complement(383256..385568)
/gene="Ace2"
/locus_tag="R008_L11226"
/note="Similar to YLR131C Transcription factor that
activates expression of early G1-specific genes, localizes
to daughter cell nuclei after cytokinesis and delays G1
progression in daughters, localization is regulated by
phosphorylation"
/codon_start=1
/product="Ace2p"
/protein_id="EWG84389.1"
/translation="MDNVVDPWYINPSGFAKDTQDEEYVQHHDNVNPTIPPPDNYILN
NENDDGLDNLLGMDYYNIDDLLTQELRDLDIPLVPSPKTGDGSSDKKNIDRTWNLGDE
NNKVSHYSKKSMSSHKRGLSGTAIFGFLGHNKTLSISSLQQSILNMSKDPQPMELINE
LGNHNTVKNNNDDFDHIRENDGENSYLSQVLLKQQEELRIALEKQKEVNEKLEKQLRD
NQIQQEKLRKVLEEQEEVAQKLVSGATNSNSKPGSPVILKTPAMQNGRMKDNAIIVTT
NSANGGYQFPPPTLISPRMSNTSINGSPSRKYHRQRYPNKSPESNGLNLFSSNSGYLR
DSELLSFSPQNYNLNLDGLTYNDHNNTSDKNNNDKKNSTGDNIFRLFEKTSPGGLSIS
PRINGNSLRSPFLVGTDKSRDDRYAAGTFTPRTQLSPIHKKRESVVSTVSTISQLQDD
TEPIHMRNTQNPTLRNANALASSSVLPPIPGSSNNTPIKNSLPQKHVFQHTPVKAPPK
NGSNLAPLLNAPDLTDHQLEIKTPIRNNSHCEVESYPQVPPVTHDIHKSPTLHSTSPL
PDEIIPRTTPMKITKKPTTLPPGTIDQYVKELPDKLFECLYPNCNKVFKRRYNIRSHI
QTHLQDRPYSCDFPGCTKAFVRNHDLIRHKISHNAKKYICPCGKRFNREDALMVHRSR
MICTGGKKLEHSINKKLTSPKKSLLDSPHDTSPVKETIARDKDGSVLMKMEEQLRDDM
RKHGLLDPPPSTAAHEQNSNRTLSNETDAL"
gene complement(386029..386901)
/locus_tag="R008_L11231"
mRNA complement(386029..386901)
/locus_tag="R008_L11231"
/product="hypothetical protein"
CDS complement(386029..386901)
/locus_tag="R008_L11231"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84390.1"
/translation="MEFISADYSSSDGSDTESESSNKSEVQIEYTEKTCIQKADSTDL
PAIPDSIILKYHIPPNLQKYEHQDMNMSRFWRSFTYFEWRPTPAIHRQLQKIICKYKE
TFMKQEYTNPYQLVDFDPLFISHLGAPKPLHVSLTRSLLFETEEQRHVFIQEMRNGLR
NNEITPFKLQICSYPKLYISERANTLYLGLPVSECPNKAQISPFKTIIAEALQKSGIS
NYQDLIVSRQNLHVSIAIASNPSKATLKRYQQLNETMGALLLLNNDFAYKLEFLVNSI
YCDENRHSIRIPFN"
gene 387191..388939
/gene="Cki1"
/locus_tag="R008_L11236"
mRNA 387191..388939
/gene="Cki1"
/locus_tag="R008_L11236"
/product="Cki1p"
CDS 387191..388939
/gene="Cki1"
/locus_tag="R008_L11236"
/note="Similar to YLR133W 'Choline kinase, catalyzing the
first step in phosphatidylcholine synthesis via the
CDP-choline (Kennedy pathway); exhibits some ethanolamine
kinase activity contributing to phosphatidylethanolamine
synthesis via the CDP-ethanolamine pathway'"
/codon_start=1
/product="Cki1p"
/protein_id="EWG84391.1"
/translation="MVQESRPGSVRSYSVGYQARSRSSSQRRHSLTRQRSSQRLIRTI
SIESDVSNITDDDDLRAVNEGVADVQLDVSETANKGPRRASATDVTDSLGSTSSEYIE
IPFVKETLDASLPSDYLKQDILNLIQSLKISKWYNNKKIQPVAQDMNLVKISGAMTNA
IFKVEYPKLPSLLLRIYGPNIDNIIDREYELQILARLSLKNIGPSLYGCFVNGRFEQF
LENSKTLTKDDIRNWKNSQRIARRMKELHVGVPLLSSERKNGSACWQKINQWLRTIEK
VDQWVGDPKNIENSLLCENWSKFMDIVDRYHKWLISQEQGIEQVNKNLIFCHNDAQYG
NLLFTAPVMNTPSLYTAPSSTSLTSQSSSLFPSSSNVIVDDIINPPKQEQSQDSKLVV
IDFEYAGANPAAYDLANHLSEWMYDYNNAKAPHQCHADRYPDKEQVLNFLYSYVSHLR
GGAKEPIDEEVQRLYKSIIQWRPTVQLFWSLWAILQSGKLEKKEASTAITREEIGPNG
KKYIIKTEPESPEEDFVENDDEPEAGVSIDTFDYMAYGRDKIAVFWGDLIGLGIITEE
ECKNFSSFKFLDTSYL"
gene 389470..391161
/gene="Pdc5"
/locus_tag="R008_L11241"
mRNA 389470..391161
/gene="Pdc5"
/locus_tag="R008_L11241"
/product="Pdc5p"
CDS 389470..391161
/gene="Pdc5"
/locus_tag="R008_L11241"
/note="Similar to YLR134W Minor isoform of pyruvate
decarboxylase, key enzyme in alcoholic fermentation,
decarboxylates pyruvate to acetaldehyde, regulation is
glucose- and ethanol-dependent, repressed by thiamine,
involved in amino acid catabolism"
/codon_start=1
/product="Pdc5p"
/protein_id="EWG84392.1"
/translation="MSEITLGKYLFERLSQVNCNTVFGLPGDFNLSLLDKLYEVKGMR
WAGNANELNAAYAADGYARIKGMSCIITTFGVGELSALNGIAGSYAEHVGVLHVVGVP
SISSQAKQLLLHHTLGNGDFTVFHRMSANISETTAMITDIANAPAEIDRCIRTTYTTQ
RPVYLGLPANLVDLNVPAKLLETPIDLSLKPNDAEAEAEVVRTVVELIKDAKNPVILA
DACASRHDVKAETKKLMDLTQFPVYVTPMGKGAIDEQHPRYGGVYVGTLSRPEVKKAV
ESADLILSIGALLSDFNTGSFSYSYKTKNIVEFHSDHIKIRNATFPGVQMKFALQKLL
DAIPEVVKDYKPVAVPARVPITKSTPANTPMKQEWMWNHLGNFLREGDIVIAETGTSA
FGINQTTFPTDVYAIVQVLWGSIGFTVGALLGATMAAEELDPKKRVILFIGDGSLQLT
VQEISTMIRWGLKPYIFVLNNNGYTIEKLIHGPHAEYNEIQGWDHLALLPTFGARNYE
THRVATTGEWEKLTQDKDFQDNSKIRMIEVMLPVFDAPQNLVKQAQLTAATNAKQ"
gene 392028..394274
/gene="Slx4"
/locus_tag="R008_L11246"
mRNA 392028..394274
/gene="Slx4"
/locus_tag="R008_L11246"
/product="Slx4p"
CDS 392028..394274
/gene="Slx4"
/locus_tag="R008_L11246"
/note="Similar to YLR135W 'Endonuclease involved in
processing DNA during recombination and repair; cleaves
branched structures in a complex with Slx1p; involved in
Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails
during DSBR via single-strand annealing'"
/codon_start=1
/product="Slx4p"
/protein_id="EWG84393.1"
/translation="MELQRAQRNLKFLQNEDYVNVTDQTNLNGESQNAYSLGMETQVP
EMQFSLSSDDDSIGTQVKSVTAQKSPMTQETTKNDTERNKDVDKSCNPVSTSHPDLGG
SNIEENIFINTQIQSRLDDAEEETNLKLKLEKFKYSFKSSNADDTHSNADVTAKRRPA
IRKANSKLKTKPKTKRDPKIIKNITDFNINNYERSRTASLLKQLSGKHKKVLDIIKTQ
NEGNSDKPPRARNNKGEKATFDTYSEQEWKDIMKLLLQKFPQSEETDLNEVQKFLYGS
EKSSNSLDNQESSQQRLWTASQLPPELPDEAIQPEQEERIRDTQSAVNFLSLSQVMDD
KSEIMKDEESIIISRGDSTSSQEYGNGLEPQQPVGNVVGEDIELAVGTRINAFSLTDY
KACKPMSVEVSRRCENSTDNDYDNISIVSDTTDETSTLFPLDQYRYVFIENDERPPLA
TDTIGSTQFFTPNTSPLDGIIDLTQESFKAVRSLISPLKVENNKTGVTSQASNQVQVP
ATRTPTIIPQKNLTTTLKTEEEKNNIGSSIRVKLLQESVVKLNPKLVKHNFYRVEAND
SEEEETEFDDQFCIADIQLVDSSKISTKDSTQNPTTSNDIIDTSAASSIASPEKFCEI
MMSQSMKELRQSLKTVGLKPMRTKVEIIQSLQTASQILSTANPDNKGEHGGVANFSKI
EIFDHLTELIEAFPDFLERIYTFEPIPLNELIEKLFSAEPFVSQIDEMTIREWADVQG
ICLRNDKK"
gene complement(394567..395424)
/gene="Tis11"
/locus_tag="R008_L11261"
mRNA complement(394567..395424)
/gene="Tis11"
/locus_tag="R008_L11261"
/product="Tis11p"
CDS complement(394567..395424)
/gene="Tis11"
/locus_tag="R008_L11261"
/note="Similar to YLR136C 'mRNA-binding protein expressed
during iron starvation; binds to a sequence element in the
3'-untranslated regions of specific mRNAs to mediate their
degradation; involved in iron homeostasis'"
/codon_start=1
/product="Tis11p"
/protein_id="EWG84394.1"
/translation="MWAQLSYTRPESQKTDLTSLFSTDQEQNPLNDYQYQINIRELEE
YYNKTILNEDNIQETSSEISSAVSFSPPKNTNAIQPGLLYDPQLMNPFLPSAHLNSTA
PTTFKKKLEVQINPDYVPKSSQLPLTSQNLQQLSQQKPKNDASFSSEKESSAQPKVKS
QVQETPKQLYKTELCESFTLKGSCPYGSKCQFAHGLGELKVKKSCKNFRTKPCVNWEK
LGYCPYGRRCCFKHGDDNDIAVYVKAGTYCNVSSTSKQSDEKRSNGRGSAKKKNLNVK
VKALQRMTW"
gene 395772..396875
/locus_tag="R008_L11266"
mRNA 395772..396875
/locus_tag="R008_L11266"
/product="hypothetical protein"
CDS 395772..396875
/locus_tag="R008_L11266"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84395.1"
/translation="MAFKLWLLDEETIYEHVFERYTQLEGQSGKLAQDLGIQDRRGGV
LEITFEPSGLEGGRKKKRVRRRNKASSVEEDQNVAVDSYHVSVGQSISSLHSSRDNGN
STTGYVLWSTTPFFINWLLYSTSAAPFRLGSQVEVTCGSSCEGHKLELPRLVDLTGAD
RGKRGILELGAGISGILPVILGNFVDTYVSTDQKGILNKLKDNIMENLSQLTRKRCIS
RSLRLELPTVEPVGDADITAASLPSKSTLHLEVAALDWEKINLQDKKTHSLHPELSLI
GETCSSVYVIAMDVIYNEYLIDPFLKTLKQLKHWLQTTYNLQFHVLVGIHLRSQEVTT
LFLEKAIIEYDFTVYDIVDQVIQESRFNFYLIT"
gene 397203..400160
/gene="Nha1"
/locus_tag="R008_L11271"
mRNA 397203..400160
/gene="Nha1"
/locus_tag="R008_L11271"
/product="Nha1p"
CDS 397203..400160
/gene="Nha1"
/locus_tag="R008_L11271"
/note="Similar to YLR138W 'Na+/H+ antiporter involved in
sodium and potassium efflux through the plasma membrane;
required for alkali cation tolerance at acidic pH'"
/codon_start=1
/product="Nha1p"
/protein_id="EWG84396.1"
/translation="MAIWEQLEVSKAHVAYACVGVFSSIFSLVSLYVKEKLYIGESTV
AGIFGLIVGPVCLNWFNPLKWGNSDSITLEITRIVLCLQIFAVAVELPRKYMLKHWVS
VTMLLLPVMTAGWLIIGLFVWILIPGLNFSASLLISACITATDPILAQSVVSGKFAQR
VPGHLRNLLSAESGCNDGMAFPFLFLSMNLILHPGNGREIVKDWICVTILYECLFGCL
LGCFIGYVGRITIRFAEKKNIIDRESFLAFYVVLAFMCAGFGSILGVDDLLVSFAAGA
TFAWDGWFSQKTQESNVSTVIDLLLNYAYFIYFGAIIPWSQFNNGEIGTNVWRLIILS
IVVIFLRRIPAVMILRPLIPDIKSWREALFVGHFGPIGVGAIFAAILARGELESTFSD
EPTPLNVVPSKEESKHWQLIACIWPITCFFIVTSIIVHGSSVAIITLGRHLNTITLTK
TFTTHTTNGDNGKSSWMQRLPSLDKAGRSFSLHRMDTQMTLSGDEGEAEEGGGRKGLA
GGEDEEGLNNDQIGSVATSGIPARPAGGMPRRRKLSRKEKRLNRRQKLRNKGREIFSS
RSKNEMYDDDELNDLGRERLQKEKEARAATFALSTAVNTQRNEEIGMGGDEEEDEYTP
EKEYSDNYNNTPSFESSERSSSLRGRTYVPRNRYDGEETESEIESEDEMENESERSMA
SSEERRIRKMKEEEMKPGTAYLDGNRMIIENKQGEILNQVDIEDRNEARDDEVSVDST
AHSSLTTTMTNLSSSSGGRLKRILTPTSLGKIHSLVDKGKDKNKNSKYHAFKIDNLLI
IENEDGDVIKRYKINPHKSDDDKSKNRPRNDSVVSRALTAVGLKSKANSGVPPPVDEE
KAIEGPSRKGPGMLKKRTLTPAPPRGVQDSLDLEDEPSSEEDLGDSYNMDDSEDYDDN
AYESETEFERQRRLNALGEMTAPADQDDEELPPLPVEAQTGNDGPGTAEGKKKQKSAA
VKSALSKTLGLNK"
gene complement(400304..402235)
/gene="Sls1"
/locus_tag="R008_L11276"
mRNA complement(400304..402235)
/gene="Sls1"
/locus_tag="R008_L11276"
/product="Sls1p"
CDS complement(400304..402235)
/gene="Sls1"
/locus_tag="R008_L11276"
/note="Similar to YLR139C 'Mitochondrial membrane protein
that coordinates expression of mitochondrially-encoded
genes; may facilitate delivery of mRNA to membrane-bound
translation machinery'"
/codon_start=1
/product="Sls1p"
/protein_id="EWG84397.1"
/translation="MWKFNKKLARLTYRLYSSSGPSSPLHGKKKLPQNLKFVVLNPTQ
SGLVKNDQKQPRHRPSKKRSHKETGDNNLDFGSKLLVFEKQNSLDSALNSIRLKKPTS
ASLPSLEYNALLQSLTSSYNRYQLREFISTHQPDSSSHLTHWKKSKLSQYIIEKIWNC
QPISTPTTPTGIKSTSLTFQFDSPREIFLLLITQNGKILTNFNKLGLTFIISIQDNEL
TVKGSPSLLKYAEISLNKIWSNITHENVRMYSLMPSKDVINLIQKETHTFFEYLPDLQ
MYKISALSTKKISMAKVFLLNAVASNPNTTQHHHTIASPALKTELYPFNNTLENLDWL
NKSQDWARLQSVVPKNCTDLMTPTENATPELTDAQVSQFESSLSKNIPSLSPSDSISQ
SLSITLGHSLQSASFSSIFQPLIHKSFISKLLNLPMYKESSSSAVPVPVPLDQHLITN
AHQSFIQLNFTPVPPTSGSSSSPFLQIWFEIDEFDNIVTTSMRPLLKLQENSVILRTP
QCQTDYKITSDYIQDLLPDFDQTNPDAWLSEQKGLQEFLLKSHWKLNKYQNLMKKINI
SLPDNLIQQYQLTDVLTHRVLNLRFPTNTAQDDKYIIQYSDISRGFLNNGSYRQLDFI
NVNPSETSLKTFINDVLSF"
gene 402442..403533
/gene="Rrn5"
/locus_tag="R008_L11286"
mRNA 402442..403533
/gene="Rrn5"
/locus_tag="R008_L11286"
/product="Rrn5p"
CDS 402442..403533
/gene="Rrn5"
/locus_tag="R008_L11286"
/note="Similar to YLR141W 'Protein involved in
transcription of rDNA by RNA polymerase I; transcription
factor, member of UAF (upstream activation factor) family
along with Rrn9p and Rrn10p'"
/codon_start=1
/product="Rrn5p"
/protein_id="EWG84398.1"
/translation="MEHQQLRKYVELYNKEVEEFYNGAASGRPAEFHPSKVHVKSIHE
KAGTANAGVEISSLGVDWDSEEKNTFFWCLSRYSIHRVDEWRSLLPRKSAMEILGYYR
LLRRASASARSRKAGDDGAPIAYEMSAEWVALETKLSETVMAITEGAAEVADEEGHCE
GLIDYESWKRRWVAIYSHSRIAEIRPLPRHALPLSRSATQTLERCVSRYTRTLLWCTA
LAGMASRSVSARAAESRGHKSLPTVVTRRQVERALCTEARSRDLHVLPRRIVLTLRKW
ELDYPREGKLFRTKEMAHLFLQSQLSRRDAPPVHQDENQENQENQENQEQDNTASEGE
SEAERDEIDEADLFRSALHENQLLKWLSK"
gene 403945..405375
/gene="Put1"
/locus_tag="R008_L11291"
mRNA 403945..405375
/gene="Put1"
/locus_tag="R008_L11291"
/product="Put1p"
CDS 403945..405375
/gene="Put1"
/locus_tag="R008_L11291"
/note="Similar to YLR142W 'Proline oxidase,
nuclear-encoded mitochondrial protein involved in
utilization of proline as sole nitrogen source; PUT1
transcription is induced by Put3p in the presence of
proline and the absence of a preferred nitrogen source'"
/codon_start=1
/product="Put1p"
/protein_id="EWG84399.1"
/translation="MIASKSSLLVTKSRIPSLCFPLIKRSYVSKTPTHSNTAANLMVE
TPAANANGNSVMAPPNSINFLQTLPKKELFQLGFIGIATLNSFFLNTIIKLFPYIPIP
VIKFFVSSLYCGGENFKEVIECGKRLQKRGISNMMLSLTIENSEGTKSLSSTPVDQIV
KETISSVHNILLPNIIGQLESKPINDIAPGYIALKPSALVDNPHEVLYNFSNPAYKAQ
RDQLIENCSKITKEIFELNQSLLKKYPERKAPFMVSTIDAEKYDLQENGVYELQRILF
QKFNPTSSKLISCVGTWQLYLRDSGDHILHELKLAQENGYKLGLKLVRGAYIHSEKNR
NQIIFGDKTGTDENYDRIITQVVNDLIINGEDSYFGHLVVASHNYQSQMLVTNLLKST
QDNSYAKSNIVLGQLLGMADNVTYDLITNHGAKNIIKYVPWGPPLETKDYLLRRLQEN
GDAVRSDNGWPLIKAIAKSIPKRVGL"
gene 405882..405953
/locus_tag="R008_L11296"
tRNA 405882..405953
/locus_tag="R008_L11296"
/product="tRNA-Asp"
/note="TD(GUC)L1"
gene 406079..408136
/locus_tag="R008_L11301"
mRNA 406079..408136
/locus_tag="R008_L11301"
/product="hypothetical protein"
CDS 406079..408136
/locus_tag="R008_L11301"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84400.1"
/translation="MKFIALISGGKDSFYNIFHCLKNNHELIALGNIYPKESEEQELD
SFMFQTVGHDLIDYYSKCIGVPLFRRSILRNTSNNVELNYTATQDDEIEELFELLRTV
KDKIPDLEAVSVGAILSSYQRTRVENVCSRLGLVVLSYLWQRDQAELMGEMCLMSKDV
NNVENDTNSGNKFDARIIKVAAIGLNEKHLGMSLPMMQPVLQKLNQLYQVHICGEGGE
FETMVLDAPFFQHGYLELIDIVKCSDGEVHNARLKVKFQPRNLSKSFLLNQLDQLPVP
SIFGNNWQDLTQNLPKQQAKTGEQRFENHMSNALPQTTINKTNDKLYISNLQSRKSET
VEKQSEDIFTELADILHSNQIPRNHILSASLLIRDMSNFGKINKIYNEFLDLSKYGPL
PPSRACVGSKCLPEDCHVQLSVVVDVKNTGKEKINKNKGGLHVQGRSYWAPCNIGPYS
QSTWLNDDANQVSFISGQIGLVPQSMEILGTPLTDQIVLALQHFDTLCETIGAQEKLL
MTCYISDESVLDSVIKTWAFYCSNMNHRSDLWMDKSDDVEKCLVLVKISELPRGAVAE
FGGVTCKRLIVDDNDSDKKEREENDDVSTVFQKLNLNIEGFHNTTVSAFGYNRNFITG
FVDSREELELILEKTPKSAQITLYYNPKEIITFHHHIGYYPVEKLFDYRGKEHRFGLH
IRS"
gene complement(408427..410766)
/gene="Acf2"
/locus_tag="R008_L11306"
mRNA complement(408427..410766)
/gene="Acf2"
/locus_tag="R008_L11306"
/product="Acf2p"
CDS complement(408427..410766)
/gene="Acf2"
/locus_tag="R008_L11306"
/note="Similar to YLR144C 'Intracellular
beta-1,3-endoglucanase, expression is induced during
sporulation; may have a role in cortical actin
cytoskeleton assembly'"
/codon_start=1
/product="Acf2p"
/protein_id="EWG84401.1"
/translation="MCYSRQAIPPPVPNRPGGTTNRGPPPLPPRANVQPPVCSSENSS
KPRENRVAGESLRTPSSSNPLADSQVNSDNIFQSPVLSNLKAPLSVFNRVQHPVPKPN
IDDQSVDPLETNKFYTNMLLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLASQRVFD
TTTNPPRFYFNPTNIKSFVFKAREFVSSNDIKLEFRDMKHMSMCLLMSLSSSQFIEFP
LVQGMGFVTAIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPD
YSFPQDFQISLLDSNTIISSHKINGLICQLSADSVPSIDMAAGCYPVYCDLSGQTVDE
HFTNYRFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLDSTVKGLMTGYLT
NSFDMQVQVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSM
YFSGKILAKYAWILYVTHYILHDENLTKELLSKLTIAMERFISNQQVLPLNYDVSWKG
IISSGSSSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVEC
LIRDYSGVDNDDPYFPQFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLW
GLVTGNSKLTDIANLQLGIMRNVFQSYFLYESNNTVQPKEFIGNKVSGILFENKIDHA
TYFGMEPQYIHMIHAIPITSASSWVRTPNFVKEEWEEKMQPIIDQVNDGWKGIIMLNM
ALLDPKFSYDFFSQPDFNRNFLDNGQSLTWSLAYSGAFS"
gene 410918..411538
/gene="Rmp1"
/locus_tag="R008_L11311"
mRNA 410918..411538
/gene="Rmp1"
/locus_tag="R008_L11311"
/product="Rmp1p"
CDS 410918..411538
/gene="Rmp1"
/locus_tag="R008_L11311"
/note="Similar to YLR145W 'Subunit of RNase MRP, which
processes pre-rRNA and has a role in cell cycle-regulated
degradation of daughter cell-specific mRNAs; unlike most
subunits, not shared between RNase MRP and nuclear RNase
P'"
/codon_start=1
/product="Rmp1p"
/protein_id="EWG84402.1"
/translation="MDEMDNVIRSLEQEYRLILLLNHRNKNQHRAASWYGSFNEMKRN
CGQIITLFSSRRLQAKRLKDVEWVKLHRLLQRALFRQLKRWYWQFNGVIALGQFVTLG
CTLVTLLANVRALYMRLWEINETEFIRCGCLIKNLPRTKVKSVVNDVEELGEIIDEDI
GNNVQENELVITSIPKPLTENCKKKKKRKRRTNQPLMAYSDKYTSK"
gene complement(411572..412474)
/gene="Spe4"
/locus_tag="R008_L11316"
mRNA complement(411572..412474)
/gene="Spe4"
/locus_tag="R008_L11316"
/product="Spe4p"
CDS complement(411572..412474)
/gene="Spe4"
/locus_tag="R008_L11316"
/note="Similar to YLR146C Spermine synthase, required for
the biosynthesis of spermine and also involved in
biosynthesis of pantothenic acid"
/codon_start=1
/product="Spe4p"
/protein_id="EWG84403.1"
/translation="MVNNSQHPYIKDGWFREINDKSFPGQAFTMTVDSILYEARSEFQ
DILIFRNKVYGTVLVLDGIVQCTEFDEFAYQEMITHIAMFAHSNPKRVLIIGGGDGGV
LREVAKHSCVEDITMVEIDSSVIELSHKFLPTLSNGAFDDERLDLKLCDGFKFLQDIG
ASDVHKKFDVIITDSSDPEGPAEAFFQERYFELLKDALNPNGVVIMQSSENFWLNLKY
LHDLKNTAKKVFPNTEYCYTMVPTYTSGQLGLIVCSNNANIPLNIPQRKISEQEQGKL
KYYNPQIHSSAFVLPTWADKVINE"
gene 412619..412807
/locus_tag="R008_L11321"
mRNA 412619..412807
/locus_tag="R008_L11321"
/product="hypothetical protein"
CDS 412619..412807
/locus_tag="R008_L11321"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84404.1"
/translation="MDTEEKKKTTASVEHARMLQNEIQQLFAQLRDTNSQIRCDLNEF
EQIKESSTTADSTTNSAN"
gene complement(412907..413212)
/gene="Smd3"
/locus_tag="R008_L11326"
mRNA complement(412907..413212)
/gene="Smd3"
/locus_tag="R008_L11326"
/product="Smd3p"
CDS complement(412907..413212)
/gene="Smd3"
/locus_tag="R008_L11326"
/note="Similar to YLR147C 'Core Sm protein Sm D3; part of
heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Sme1p,
Smx3p, and Smx2p) that is part of the spliceosomal U1, U2,
U4, and U5 snRNPs; homolog of human Sm D3'"
/codon_start=1
/product="Smd3p"
/protein_id="EWG84405.1"
/translation="MTMNGIPVKLLNEAQGHIVSLELTTGATYRGKLVESEDSMNVQL
RDVIATEPQGAVTHMDQIFVRGSQIKFIVVPDLLKNAPLFKKNSSRPMPPIRGPKRR"
gene 413390..416146
/gene="Pep3"
/locus_tag="R008_L11331"
mRNA 413390..416146
/gene="Pep3"
/locus_tag="R008_L11331"
/product="Pep3p"
CDS 413390..416146
/gene="Pep3"
/locus_tag="R008_L11331"
/note="Similar to YLR148W 'Component of CORVET tethering
complex; vacuolar peripheral membrane protein that
promotes vesicular docking/fusion reactions in conjunction
with SNARE proteins, required for vacuolar biogenesis'"
/codon_start=1
/product="Pep3p"
/protein_id="EWG84406.1"
/translation="MIKTRIEEVQLQFLTGNTELTHLKVSNDQLIVTTQRTIYRINLQ
DPAIVNHFDCPLSKELETIMNVHVSPMGSVILIRTNFGRYMLLKDGEFTQLNKIKNLD
LSSLHWINETTFLMGIKKTPKLYRVELTGKDITTKLWYENKKLSGGIDGIAYWEGSLL
LTIKDNILYWRDVTNMKFPLVLPDESEQFERLKHHAIKKFDSYNGLFAWVTSNGIVFG
DLKEKQMEKDPASNNFGKFLSSSKVLLNFELPDYQNDKDHLIKDIVLTAFHILLLRKN
TVTMVSQLNNDVVFHETIPRHQLTGSNTDSNEKFLGLVRDSVKETFWCFSNINVFEII
IENEPNSVWNLLVRDNKFDKALSLKGLTVREIESVKLSKAMYLFHTAKDFHSAAQTLG
SMKDLSHFGEIALNFLQIKDYNDLNVILIKQLDNVPWKSTQVVLSSWIIWNFMKQLND
IELKINTTKPASTDEDNLLNWNLNLKEKSNELTKFLESHLEKLDNETVYQIMSKQNRQ
NELLIFASLINDMKFLLSFWIDQGNWYESLKILLTINNHDLVYKYSLILLLNSPEATV
STWMKIKDLDPNKLIPTILKFFTNWQNNSKLITNISEYPENYSLTYLKWCVREVPKMC
NPIVYNSILYMMITDPRNDMILENDIIKFMKSNENKYDLNFQLRLSLKFKKTKTSIFL
LTRLNLFEDAIDLALKNNLIDDCKVIVNDEILIEDYKLRKRLWLKIAKHLLLSMKDID
IKQLIRTILNDSNEILTIKDLLPFFNEYTTIANLKEELIKFLENHNMKMNEISEDIIN
SKNLKVEINTEISKFNEIYRILEPGKSCDECGKFLQIKKFIVFPCGHCFHWNCIIRVI
LNSNDYNLRQKTENFLKAKSKHNLNDLENIIVEKCGLCSDININKIDQPISIDETELA
KWNE"
gene complement(416380..418572)
/locus_tag="R008_L11336"
mRNA complement(416380..418572)
/locus_tag="R008_L11336"
/product="hypothetical protein"
CDS complement(416380..418572)
/locus_tag="R008_L11336"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84407.1"
/translation="MTIDGTGQSKEALQDERLNTGSDKVYQNYMMPALELYDAKVSIN
HWQLRDCIKPGSMNQSKLYYIYDHSIRVLDTDSSVLRSPVRRHNSIQPSNSGKNSTEK
TSTKGSRTTGSYISKNLHVPSEKLVEFNFKPRCFTELNGLTVCGGLIGSDDKGFPSNW
NRLAQDANISLPPPSQPINISKNISFPINSHYSNPNIWKGIVEFYNQETDTMMTFTLG
QFINNCVTLYDRASMQFDLFACNNDGHLYQCDVSNRDVTLVKRYADLKFPLNNASLSH
DGQTMVVSGDSNKFAVYNQNELTNQFSLHYDNHPSWGSSVNRVRRIPRFALPDESEYI
ENIYEAPNSDHGFYNCFSENDLQFATVFQNGTCAIYDIRNMATPMAEISSTRPHSHNG
AFRVCRFSYGLDDLLFISEHQGRVHVVDTRNYVNHQVIVIPDKVNMEYINERKHNTNH
NFTTNNNNENESNDSKNELQGADYRSLSRRRFSLPSMPNVTTEPWITMAQRIPKKYLE
PQILPFPKVMDKISNESVLFSTKGSSSSDVAHPYKRRCSFRVRRVSTSAPTADYSNNN
VNASLGTPAADSIATSSSNSAPQNLIDPLILSHQQASNDVFEDDEYYEAYNDVHSTYR
VSSDYHGVSARAFESFLRPPSTPDLPSDDDNFAANSRNNRGTSNFLRRPVITTQESNE
FSEENNISGIDWVEDRNGSSLIIGTDYGIMRWNINSWARRSFSSYDLC"
gene complement(419118..419204)
/locus_tag="R008_L11341"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 419216..420037
/gene="Stm1"
/locus_tag="R008_L11346"
mRNA 419216..420037
/gene="Stm1"
/locus_tag="R008_L11346"
/product="Stm1p"
CDS 419216..420037
/gene="Stm1"
/locus_tag="R008_L11346"
/note="Similar to YLR150W 'Protein required for optimal
translation under nutrient stress; perturbs association of
Yef3p with ribosomes; involved in TOR signaling; binds G4
quadruplex and purine motif triplex nucleic acid; helps
maintain telomere structure'"
/codon_start=1
/product="Stm1p"
/protein_id="EWG84408.1"
/translation="MSNPFDLLGNDVEDADVVVLPPKEIVKSNTSSKKADVPPPSADP
SKARKNRPRPSGNEGAIRDKTAGRRNNRSKDVTDSATTKKSNTRRATDRHSRTGKTDT
KKKVNQGWGDDKKELSAEKEAQADAAAEIAEDAAEAEDAGKPKTAQLSLQDYLNQQAN
NQFNKVPEAKKVELDAERIETAEKEAYVPATKVKNVKSKQLKTKEYLEFDATFVESNT
RKNFGDRNNNSRNNFNNRRGGRGARKGNNTANATNSANTVQKNRNIDVSNLPSLA"
gene complement(420464..421486)
/gene="Pcd1"
/locus_tag="R008_L11351"
mRNA complement(420464..421486)
/gene="Pcd1"
/locus_tag="R008_L11351"
/product="Pcd1p"
CDS complement(420464..421486)
/gene="Pcd1"
/locus_tag="R008_L11351"
/note="Similar to YLR151C Peroxisomal nudix
pyrophosphatase with specificity for coenzyme A and CoA
derivatives, may function to remove potentially toxic
oxidized CoA disulfide from peroxisomes to maintain the
capacity for beta-oxidation of fatty acids"
/codon_start=1
/product="Pcd1p"
/protein_id="EWG84409.1"
/translation="MILSQRRMLSSKQLIENLIRYKFHKTPYTRSSIWPFKRNSAVII
LLFIGMKGELRVLLTKRSRTLRSFSGDVSFPGGKADYFQETFESVARREAEEEIGLPH
DPEVLHKEFGMKLDNLVMDMPCYLSRTFLSVKPMVCFLYKDKLEKHEDKYKVPLDIRK
FFGKLNPGETSSLFSVPLNDLVIHLLPEADEDVKSYQAEYFERKEYKLNWGGIKWLIM
HYHFHVANNNEMPWLQTIEDLSSSDEDGVDGGIFRFRDLWGLTCKILFDVSCIANGLM
DEKLKGELGHEDLIVGLHDYGNQMQPNGRSEWEIGMINGDRNLKYSDVIPEYYMKHLL
ECRSLW"
gene complement(421707..423437)
/locus_tag="R008_L11356"
mRNA complement(421707..423437)
/locus_tag="R008_L11356"
/product="hypothetical protein"
CDS complement(421707..423437)
/locus_tag="R008_L11356"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84410.1"
/translation="MSLSLGAAIYIALKPIFKIYTIMLVGYLVAKFDIVSMENAKGIS
NMVVNAILPCLTFNKIVSNISWRDIKEIGVIILSAFILFVLGATGALFTTFATTVPKK
FFWGLIFAGFFPNISDLPIAYIQSMGNGSIFTAEEADKGVAYSCIFLFIQSFLMMNFG
MWRVVGLDFRDTKGPDSENITPSVSPANDDRKLTEITKLPNITRPTNAYQSEDARSNS
DLSCNSITTNEMTPQAYYEGFTGYIKPYKESNGASHKFESDLPHAEIYRVSSTYSSPG
ALEFSRIDGSSLSYSRISKNSDGRSYRRKRKADMNELISKYSAAEKIRQGELDLSRPL
SLTEEVGSRNASIGNVHTGYTDESSIEEENCTNMATDGRGSLSFFIERHNLKWLQYFI
INCLRPASLGAILGIICALIPWVKACFVTTYVHVHKAPDGEPVLNFLMDFTEYIGNAC
VPLGLLLLGGTLARLEIKSLPPGFIKSALLMTCFRLIVIPIIGVLWVNKLYSIDWLDT
GIGKFDMILTWSMPSATAQVYFTAFYTPACGDHIQMNCLSVLFVMQYAILFITVAFVV
TYTLKVDLKV"
gene complement(424273..426324)
/gene="Acs2"
/locus_tag="R008_L11361"
mRNA complement(424273..426324)
/gene="Acs2"
/locus_tag="R008_L11361"
/product="Acs2p"
CDS complement(424273..426324)
/gene="Acs2"
/locus_tag="R008_L11361"
/note="Similar to YLR153C 'Acetyl-coA synthetase isoform
which, along with Acs1p, is the nuclear source of
acetyl-coA for histone acetylation; mutants affect global
transcription; required for growth on glucose; expressed
under anaerobic conditions'"
/codon_start=1
/product="Acs2p"
/protein_id="EWG84411.1"
/translation="MTIKEHKVVHEAHNVKALKAPQHFYNSQPGKGYVTDMQHYQEMY
QQSINEPEKFFDKMAKEYLHWDAPYTKVQSGSLNNGDVAWFLNGKLNASYNCVDRHAF
ANPDKPALIYEADDESDNKIITFGELLRKVSQIAGVLKSWGVKKGDTVAIYLPMIPEA
VIAMLAVARIGAIHSVVFAGFSAGSLKDRVVDANSKVVITCDEGKRGGKTINTKKIVD
EGLNGVDLVSRILVFQRTGTEGIPMKAGRDYWWHEEAAKQRTYLPPVSCDAEDPLFLL
YTSGSTGSPKGVVHTTGGYLLGAALTTRYVFDIHPEDVLFTAGDVGWITGHTYALYGP
LTLGTASIIFESTPAYPDYGRYWRIIQRHKATHFYVAPTALRLIKRVGEAEIAKYDTS
SLRVLGSVGEPISPDLWEWYHEKVGNKNCVICDTMWQTESGSHLIAPLAGAVPTKPGS
ATVPFFGINACIIDPVTGVELEGNDVEGVLAVKSPWPSMARSVWNHHDRYMDTYLKPY
PGHYFTGDGAGRDHDGYYWIRGRVDDVVNVSGHRLSTSEIEASISNHENVSEAAVVGI
PDELTGQTVVAYVSLKDGYLQNNATEGDAEHITPDNLRRELILQVRGEIGPFASPKTI
ILVRDLPRTRSGKIMRRVLRKVASNEAEQLGDLTTLANPEVVPAIISAVENQFFSQKK
K"
gene complement(426732..427064)
/gene="Rnh203"
/locus_tag="R008_L11366"
mRNA complement(426732..427064)
/gene="Rnh203"
/locus_tag="R008_L11366"
/product="Rnh203p"
CDS complement(426732..427064)
/gene="Rnh203"
/locus_tag="R008_L11366"
/note="Similar to YLR154C 'Ribonuclease H2 subunit,
required for RNase H2 activity; related to human AGS3 that
causes Aicardi-Goutieres syndrome'"
/codon_start=1
/product="Rnh203p"
/protein_id="EWG84412.1"
/translation="MTKDAVNLDAYTVSFMPFYTEYQGPTEEFKDYKFEDTIYFRGKE
LKREKSATPSSSDNTTSNTFSNGAILSGNTITGKIVSVNNYEREGTDRNELARLQELI
SLIDVINQ"
gene complement(427401..427472)
/locus_tag="R008_L11371"
tRNA complement(427401..427472)
/locus_tag="R008_L11371"
/product="tRNA-Gln"
/note="TQ(UUG)L"
assembly_gap 429961..433177
/estimated_length=3217
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 436029..440069
/estimated_length=4041
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 441075..441634
/estimated_length=560
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 442698..442811
/locus_tag="R008_L11381"
mRNA 442698..442811
/locus_tag="R008_L11381"
/product="hypothetical protein"
CDS 442698..442811
/locus_tag="R008_L11381"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84413.1"
/translation="MMTSLSLSIALLSKTDLVKISLRISTAFGISSCRDLA"
gene 443768..444274
/locus_tag="R008_L11386"
mRNA 443768..444274
/locus_tag="R008_L11386"
/product="hypothetical protein"
CDS 443768..444274
/locus_tag="R008_L11386"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84414.1"
/translation="MSSTKFLKPLCRIRAFHTSIARSFTIPFLPKIPQKPGGVSGTAN
DSSYMPPESRAQGSYHWIVERGLSLAVLPLIAVPLVTTGPISTFTDTFLSLVLLGHCH
IGFQSCIIDYISERVYGKVHHYAMYLLSLGSFLSFVGIYKLESQEAGLIASLKSLWDN
KPVEKKRQ"
gene complement(444379..445143)
/gene="Pus5"
/locus_tag="R008_L11391"
mRNA complement(444379..445143)
/gene="Pus5"
/locus_tag="R008_L11391"
/product="Pus5p"
CDS complement(444379..445143)
/gene="Pus5"
/locus_tag="R008_L11391"
/note="Similar to YLR165C 'Pseudouridine synthase,
catalyzes only the formation of pseudouridine (Psi)-2819
in mitochondrial 21S rRNA; not essential for viability'"
/codon_start=1
/product="Pus5p"
/protein_id="EWG84415.1"
/translation="MSLKKQIPIIFENTHYFIVNKPPGIPSQPPDCRTWGRTHPNLDP
TPLLERFKANYYSHREVELCRTVHRLDHCVTGGMLIAKTKDASVKFSRFLQKGGNNGY
KLQRKYVAIVESSGRFNKPNNYEIKYGPKYNFLISHGGREITKFKEVDENCIVLQLVT
GKKHQIRNHVSQILNQPILNDKKYGSTVNFPELFNDQIALHSACIITKIGLQTKTHLI
PMEHNNTGQLWSRKYVNEEGEFTLPIKEVLLENWDQ"
gene complement(445315..447930)
/gene="Sec10"
/locus_tag="R008_L11396"
mRNA complement(445315..447930)
/gene="Sec10"
/locus_tag="R008_L11396"
/product="Sec10p"
CDS complement(445315..447930)
/gene="Sec10"
/locus_tag="R008_L11396"
/note="Similar to YLR166C Essential 100kDa subunit of the
exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p,
Sec15p, Exo70p, and Exo84p), which has the essential
function of mediating polarized targeting of secretory
vesicles to active sites of exocytosis"
/codon_start=1
/product="Sec10p"
/protein_id="EWG84416.1"
/translation="MNSLYELDPKWKKLLKTDNFLGGLTVNEFVQELSKDHRNDVLID
ANTKNLPTNEKDQDAIREAIWKQLDPKPYIRTFESTLKELKNLNEETLNKRQYFSEQV
ATQEVIHSENVIKLSKDLHTTLLTFDKLDDRLTNVTQVVSPLGDKLETAIKKKQNYIQ
SVELIRRYNDFYSMGKSDIVEQLRLSKNWKLNLKSVKLMKNLLILSSKLETSSIPKTI
NTKLVIEKYSEMMENELLENFNSAYRENNFTKLNEIAIILNNFNGGVNVIQSFINQHD
YFIDTKQIDLENEFENVFIKNVKFKEQLIDFENHSVIIETSMQNLINDVETVIKNESK
IVKRVFEEKATHVIQLFIQRVFAQKIEPRFEVLLRNSLSISNLAYVRILHGLFTLFGK
FTKSLIDYFQLLEIDDSNQILSTTLEQCFADLFSHYLYDRSKYFGIEKRSLEAILVDM
TSKFTVNYDKEINKRVLLDKYKEKLSTNVDAFMHSPRGNTHSRQDSTSRSKLSQFNSF
LKTHLDKDHLSLNRTNTLSDSFNNSSSSTQYDVANNPSSLVNSSFTASDIDNSPNSPA
NYSLNDVDSMLKCVVESTARVMELIPNKAHLYILEILKIMFLGIVDSYMEIALEVAYW
KICKVDINKTAGVVNLNFLKFISMSTEILDLLSISIKSIFLPLLNNSPEIKAQIIEMT
NSEIQKMEILINIILQETITVISTKFSAILCKQKKKDFVPKSQELLDQDTLPAIEIVN
ILNLIFEQSSKFLKGKNLQTFLTLIGEELYGLLLSHYSHFQVNSIGGVVVTKDIIGYQ
TAIEDWGVASLIDKFATLRELANLFTVQPELLESLTKEGHLADIGRDIIQSYISNRED
FNHDNFINSVKLNFR"
gene 448841..449299
/gene="Rps31"
/locus_tag="R008_L11411"
mRNA 448841..449299
/gene="Rps31"
/locus_tag="R008_L11411"
/product="Rps31p"
CDS 448841..449299
/gene="Rps31"
/locus_tag="R008_L11411"
/note="Similar to YLR167W 'Fusion protein that is cleaved
to yield a ribosomal protein of the small (40S) subunit
and ubiquitin; ubiquitin may facilitate assembly of the
ribosomal protein into ribosomes; interacts genetically
with translation factor eIF2B'"
/codon_start=1
/product="Rps31p"
/protein_id="EWG84417.1"
/translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLI
FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGGKKRKKKVYTTPKKIKHKHKKVKLAV
LSYYKVDAEGKVTKLRRECSNPTCGAGVFLANHKDRLYCGKCHSVYKVNA"
gene complement(449472..450164)
/gene="Aim30"
/locus_tag="R008_L11416"
mRNA complement(449472..450164)
/gene="Aim30"
/locus_tag="R008_L11416"
/product="Aim30p"
CDS complement(449472..450164)
/gene="Aim30"
/locus_tag="R008_L11416"
/note="Similar to YLR168C 'Mitochondrial intermembrane
space protein involved in regulation of mitochondrial
cardiolipin and phosphatidylethanolamine levels; null has
defects in mitochondrial morphology; similar to Ups1p,
Ups3p and to human PRELI'"
/codon_start=1
/product="Aim30p"
/protein_id="EWG84418.1"
/translation="MKLFQNSYDFNYPWDQVTAANWKKYPNEISTHVIAVDVLRRELK
DQGKVLVTERLITVKQGVPKWIMMMLGGTNMSHVREVSVVDLNKKSLTMRSCNLTMCN
LLKVYETVTYSPHPDDSANKTLFQQEAQITAYGSIRKLCNKMEDWSVQRFCENAKKGK
MGFDAVLQVFSENWEKHVDDLSNQLVSKVNETMEDVKISAGTLLKGTERSGRTILQQN
IDLFRDAYNHEN"
gene complement(450473..450953)
/gene="Aps1"
/locus_tag="R008_L11426"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(451164..452066)
/gene="Dph5"
/locus_tag="R008_L11436"
mRNA complement(451164..452066)
/gene="Dph5"
/locus_tag="R008_L11436"
/product="Dph5p"
CDS complement(451164..452066)
/gene="Dph5"
/locus_tag="R008_L11436"
/note="Similar to YLR172C 'Methyltransferase required for
synthesis of diphthamide, which is a modified histidine
residue of translation elongation factor 2 (Eft1p or
Eft2p); not essential for viability; GFP-Dph5p fusion
protein localizes to the cytoplasm'"
/codon_start=1
/product="Dph5p"
/protein_id="EWG84419.1"
/translation="MLYLIGLGLSYKSDITVRGLEAIKKCSRVYLEHYTSILMAASQE
ELESYYGKEIILADRELVETGSKQILNNADKEDVAFLVVGDPFGATTHTDLVLRAKRE
AIPVEIIHNASVMNAVGACGLQLYNFGQTVSMVFFTDNWRPDSWYDKIWENRKIGLHT
LVLLDIKVKEQSIENMARGRLIYEPPRYMSIAQCCEQLLEIEEKRGTKAYTPDTPAVA
ISRLGSSSQSFKSGTISELANYDSGEPLHSLVILGRQCHELELEYLLEFADDKEKFGK
DVANDQEYFKPAAWVPPTEDDSDE"
gene 452324..454150
/locus_tag="R008_L11446"
mRNA 452324..454150
/locus_tag="R008_L11446"
/product="hypothetical protein"
CDS 452324..454150
/locus_tag="R008_L11446"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84420.1"
/translation="MHTNSPLRADNQDLETQPLLRPNTEESQLLNDEVRINVANETLI
KSRWRSIKCLIIYLLGIILLSFFGISIVQYIRGHVPPTDVIEKNLVQVTNFKLVEFQL
DGWKDNMGSDLNNDTGKYLQVSIHSQIWFDYDKWPGTENDSDARSQRDWIRYINENVL
KTICIDLNNVTTFDGDLVFKNKLGDVVGMEPICFNLAHRQINNLQFKILVKPSIWKIV
KVLKKFWNRDFESLNIKSNLDMTIFKRKFGTRFNLLILNGEILNWKDIIDWEKISATP
LRMIQNMIDAISLQGFTLRDSNSDGFHADMRLNPITILGGVDWLHLPPGTSIPFINWE
IKLPDCNGEPAIAIPTLSCFNEPINLHHDNDNIVVCLQNEIEGPLPDELLYQECPQNS
LTPMSQIVNAVLNQNETVTFAARGHVLEDGIDNNSLIPADMLEDIFQEASFIPITTNA
TFNSSELIQEFQINDLQLRWAARKKLSLVGTFLGFFDLSFYETHQQDRVRIDTIRGQI
DLYHNDINFLNLPMKQWINSSSHILHDEDTGNTQMKLQFDLENDDMEVVNSLELTRTL
NEILFQGFTVIHFNATIDASLTTALGPWVLTGLAGEGDTLVT"
gene 454495..455733
/gene="Idp2"
/locus_tag="R008_L11451"
mRNA 454495..455733
/gene="Idp2"
/locus_tag="R008_L11451"
/product="Idp2p"
CDS 454495..455733
/gene="Idp2"
/locus_tag="R008_L11451"
/note="Similar to YLR174W 'Cytosolic NADP-specific
isocitrate dehydrogenase, catalyzes oxidation of
isocitrate to alpha-ketoglutarate; levels are elevated
during growth on non-fermentable carbon sources and
reduced during growth on glucose'"
/codon_start=1
/product="Idp2p"
/protein_id="EWG84421.1"
/translation="MTKIKVANPIVEMDGDEQTRIIWHLIRDKLVLPYLDVDLKYYDL
SVEYRDQTNDQVTVDSATATLKYGVAVKCATITPDEARVEEFHLKKMWKSPNGTIRNI
LGGTVFREPIIIPRIPRLVPQWEKPIIIGRHAFGDQYKATDVIVPEEGELRLVYKSKS
GTHDVDLKVFDYPEHGGVAMMMYNTTDSIEGFAKASFELAIERKLPLYSTTKNTILKK
YDGKFKDVFEAMYARSYKEKFESLGIWYEHRLIDDMVAQMLKSKGGYIIAMKNYDGDV
ESDIVAQGFGSLGLMTSVLITPDGKTFESEAAHGTVTRHFRQHQQGKETSTNSIASIF
AWTRGIIQRGKLDNTPDVVKFGQILESATVNTVQEDGIMTKDLALILGKSERSAYVTT
EEFIDAVESRLKKEFEAAAL"
gene 456039..457499
/gene="Cbf5"
/locus_tag="R008_L11456"
mRNA 456039..457499
/gene="Cbf5"
/locus_tag="R008_L11456"
/product="Cbf5p"
CDS 456039..457499
/gene="Cbf5"
/locus_tag="R008_L11456"
/note="Similar to YLR175W 'Pseudouridine synthase
catalytic subunit of box H/ACA small nucleolar
ribonucleoprotein particles (snoRNPs), acts on both large
and small rRNAs and on snRNA U2; mutations in human
ortholog dyskerin cause the disorder dyskeratosis
congenita'"
/codon_start=1
/product="Cbf5p"
/protein_id="EWG84422.1"
/translation="MSKEDFVIKPEAAGASTDTSEWPLLLKNFDKLLVRSGHYTPIPA
GSSPLKRDLKSYISSGVINLDKPSNPSSHEVVAWIKRILRCEKTGHSGTLDPKVTGCL
IVCIDRATRLVKSQQGAGKEYVCIVRLHDALKDEKDLGRSLENLTGALFQRPPLISAV
KRQLRVRTIYESNLIEFDNKRNLGVFWASCEAGTYMRTLCVHLGMLLGVGGHMQELRR
VRSGALSENDNMVTLHDVMDAQWVYDNTRDESYLRSIIQPLETLLVGYKRIVVKDSAV
NAVCYGAKLMIPGLLRYEEGIELYDEIVLITTKGEAIAVAIAQMSTVDLASCDHGVVA
SVKRCIMERDLYPRRWGLGPVAQKKKQMKADGKLDKYGRVNENTPEQWKKEYVPLDNA
EQSTSSSQETKETEEEPKKAKEDSLIKEVETEKEEVKEDDSKKEKKEKKDKKDKKEKK
EKKDKKDKKEKKEKKEKKRKSEDGDSEEKKSKKSKK"
gene complement(457712..460146)
/gene="Rfx1"
/locus_tag="R008_L11461"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 460969..462852
/locus_tag="R008_L11471"
mRNA 460969..462852
/locus_tag="R008_L11471"
/product="hypothetical protein"
CDS 460969..462852
/locus_tag="R008_L11471"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84423.1"
/translation="MELPSINSTTSISDNQELRNYYDKLLFKNNSGKSLADLPGKMAD
FNDNSAAAHPRSRVDFINGYIGFREDKQSLLGQKNTKRASFSAFADEGRKQNEMSING
KSPNLSLFSFEFNGTPTQDRKPYKQDYLNVMNTSPNNILSPLNNSSQKYYPQKQQQQQ
QQQQQQQQSIFDPGRRSSYISDALIHGNAATQQPQYSQPVYINNNPSLQVPYTAPSEY
TQQQQYSSPFNARRNTQPVLNLHPAAAPTNDAGLAVVDGKNLTSSKELHDLYLDCGSN
YFASDKVYKFIDSIKGTLRGDNVSASSSRIIEFLDFLKNCNLNYNPQSDAFISTAVSN
ASSTGAAKSKNSTSMHLHYKPLVLVSLKNGKLELLSKPQTATLILKRGDLVIIDGDRG
KDLVLVVEPVVDINLALFINFLKKKIHFDSLITNSQQHFPNDQFIKTLVDTTNGKPVA
HELNPKLYDIIELTQLIIPSKQVLRFATPWESSTNLHNKFQDELKALHIAQLKLRSLN
NNNSGGGLNIKILNAEFQFDRKKLTFYYICQERNDFRDLIKELFKFYKTRIWLCAIPN
NLSIDSKFYDSNKFEWEMYQDMMSHYSMDNTGIVVAPELNRLKLDDFQIGVYMELVKV
LFG"
gene complement(462925..463692)
/gene="Tfs1"
/locus_tag="R008_L11476"
mRNA complement(462925..463692)
/gene="Tfs1"
/locus_tag="R008_L11476"
/product="Tfs1p"
CDS complement(462925..463692)
/gene="Tfs1"
/locus_tag="R008_L11476"
/note="Similar to YLR178C 'Protein that interacts with and
inhibits carboxypeptidase Y and Ira2p;
phosphatidylethanolamine-binding protein (PEBP) family
member; targets to vacuolar membranes during stationary
phase; acetylated by NatB N-terminal acetyltransferase'"
/codon_start=1
/product="Tfs1p"
/protein_id="EWG84424.1"
/translation="MNQAIDFAQASIDSYKKHGILEDVIHDTSFQPSGILAVEYSSSA
PVAMGNTLPTEKAHSKPQFQFTFNKQMQKSVPQANAYVPQDDDLFTLVMTDPDAPSKT
DHKWSEFCHLVECDLKLLNEATHETSGATEFFASEFNTKGSNTLIEYMGPAPPKGSGP
HRYVFLLYKQPKGVDSSKFSKIKDRPNWGXXXXXXXXXXXXXXXXIYIYIYICICIYF
LDFSAFHLTFYYFCFIYVFVTNAHMFVGSNVIVKQNT"
assembly_gap 463079..463124
/estimated_length=46
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(463977..464582)
/locus_tag="R008_L11486"
mRNA complement(463977..464582)
/locus_tag="R008_L11486"
/product="hypothetical protein"
CDS complement(463977..464582)
/locus_tag="R008_L11486"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84425.1"
/translation="MSSAIVAKLNKEDIIKDTVKDLAFEIHGELSVSYVDSDDIKLGN
AMPMEATQAAPTIKFTPFDKSQLSAEDKLALLMTDPDAPSRTEHKWSEVCHYIITDIP
VEYGPGGDIAISGKGVVRNNYIGPGPPKNSGYHRYVFFLCKQPKGADSSTFTKVENKV
SWGYGTPGAGAYDYIKENNLQLVGANYYMVENTTVDFNYDM"
gene 465128..466276
/gene="Sam1"
/locus_tag="R008_L11491"
mRNA 465128..466276
/gene="Sam1"
/locus_tag="R008_L11491"
/product="Sam1p"
CDS 465128..466276
/gene="Sam1"
/locus_tag="R008_L11491"
/note="Similar to YLR180W 'S-adenosylmethionine
synthetase, catalyzes transfer of the adenosyl group of
ATP to the sulfur atom of methionine; one of two
differentially regulated isozymes (Sam1p and Sam2p)'"
/codon_start=1
/product="Sam1p"
/protein_id="EWG84426.1"
/translation="MAGTFLFTSESVGEGHPDKICDQVSDAILDACLAEDPHSKVACE
TAAKTGMIMVFGEITTKAQLDYQKIVRDTIKKIGYDDSAKGFDYKTCNVLVAIEQQSP
DIAQGVHEEKDLEDIGAGDQGIMFGYATDETPEGLPLTILLAHKLNMAMADARRDGSL
AWLRPDTKTQVTVEYKDDHGRWVPQRIDTVVVSAQHADEITTEDLRAQLKSEIIEKVI
PRDMLDENTKYFIQPSGRFVIGGPQGDAGLTGRKIIVDAYGGASSVGGGAFSGKDYSK
VDRSAAYAARWVAKSLVAAGLCKRVQVQFSYAIGIAEPLSLHVDTYGTATKSDEEIID
IISKNFDLRPGVLVKELDLARPIYLPTASYGHFTNQEYPWEKPKTLKF"
gene complement(466544..467536)
/gene="Vta1"
/locus_tag="R008_L11496"
mRNA complement(466544..467536)
/gene="Vta1"
/locus_tag="R008_L11496"
/product="Vta1p"
CDS complement(466544..467536)
/gene="Vta1"
/locus_tag="R008_L11496"
/note="Similar to YLR181C 'Multivesicular body (MVB)
protein involved in endosomal protein sorting; regulates
Vps4p activity by promoting its oligomerization; has an
N-terminal Vps60- and Did2- binding domain, a linker
region, and a C-terminal Vps4p binding domain'"
/codon_start=1
/product="Vta1p"
/protein_id="EWG84427.1"
/translation="MASNAARVVATAKDFDKVGLGIIGYYLQLYVVELILSEEDRSQE
MTALATELLDTIEAFKKEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYN
EKLKQLKDGPWDVMLKRSLWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSK
LAKGEIGSSDEKTLDYADFADDSEETKDEDVDYQTSDLENNNNDKVEGLAPKDQTTSY
EPVDEVPEFIDDADSVNEEEQTVDKNEDAITKDEQQVVKKKVDLTRPSAPSEPAAAEH
KSYTKDELTKIMDRASKIEQIQKLAKYAISALNYEDLPTAKDELTKALDLLNSI"
gene 467826..470237
/gene="Swi6"
/locus_tag="R008_L11501"
mRNA 467826..470237
/gene="Swi6"
/locus_tag="R008_L11501"
/product="Swi6p"
CDS 467826..470237
/gene="Swi6"
/locus_tag="R008_L11501"
/note="Similar to YLR182W 'Transcription cofactor, forms
complexes with Swi4p and Mbp1p to regulate transcription
at the G1/S transition; involved in meiotic gene
expression; cell wall stress induces phosphorylation by
Mpk1p, which regulates Swi6p localization'"
/codon_start=1
/product="Swi6p"
/protein_id="EWG84428.1"
/translation="MALEEVVRYLGPHNEIPLTLTRDSETGHFLLKHFLPILQQYHDT
GNINETNPDSFPTDEERNKLLAHYGIAVNTDDRGELWIELEKCLQLLNMLNLFRLFQD
AFEFEEPETDQDEEDPSHSKLPENKTKSENSKDNISSKRINNLQDMSLDSDAHRELGS
PLKKLKIDTSVIDAESDSTPNTARGKPNDDINKGPSGDNENNGTDDSDRTAGPIITFT
HDLTSDFLSSPLKIMKALPSPVVNDNEQKMKLEAFLQRLLFPEIQEMPTSLNNDSSNR
NSEGGSSNQQQQHVSFDSLLQEVNDAFPNTQLNLNIPVDEHGNTPLHWLTSIANLELV
KHLVKHGSNRLYGDNMGESCLVKAVKSVNNYDSGTFEALLDYLYPCLILEDSMNRTIL
HHIIITSGMTGCSAAAKYYLDILMGWIVKKQNRPIQSGTNEKESKPNDKNGERKDSIL
ENLDLKWIIANMLNAQDSNGDTCLNIAARLGNISIVDALLDYGADPFVANKSGLRPVD
FGAGTSKLQNTNGGDENSKMVSKGDYDGQKNGKAKKIRSQLLKNPPETTSLINDVQNL
LNSISKDYESETVQYNEKLEKLHKELNEQREELANSREQLANVKQLKDEYSLMQEQLT
NLKAGIEEEEESFREESKKLGIIADESSGIDWDSSEYDADEPFKVEFLSDFLEDKLQK
NYEGDISKLLEAESKEQIMEQIRNQIPAEKIQSMLPPTVLLKARINAYKRNDKHLTNV
LDTISTKQSELENKFRRVLSLCLKIDENKVDNMLDGLLQAISSEDPQDIDTDEMQDFL
KKHAS"
gene complement(470428..471897)
/gene="Tos4"
/locus_tag="R008_L11506"
mRNA complement(470428..471897)
/gene="Tos4"
/locus_tag="R008_L11506"
/product="Tos4p"
CDS complement(470428..471897)
/gene="Tos4"
/locus_tag="R008_L11506"
/note="Similar to YLR183C 'Forkhead Associated domain
containing protein and putative transcription factor found
associated with chromatin; target of SBF transcription
factor; expression is periodic and peaks in G1; similar to
PLM2'"
/codon_start=1
/product="Tos4p"
/protein_id="EWG84429.1"
/translation="MSSQFPSSPYRTVDPYSPPNYKQQPNCPSSNYEKAGKTASESIG
NFGKGDYPTPFPSSSIGRVSSPVRSNKVDAIPSSPAFPGQLAETSPKFSSKLSSPSRH
TRVINAELDPSKISTITVGRNGSQCDVALCKNKFISRVHASITYLPQTNEVKIHCFSM
NGLIVTYRKQFDCYQLKDTMNNNNRAYRLVPRFSNEKCVKEIQDEGGFINFTLEEGDT
VYMTYYKGIMLDFRQVLLRISLKEKNSPSEPLRFEKKAEFESESETKHMGSIRKHPLI
FTDTSMDRPKKILKDSNKISIGSDSGVAERMLNHFLNSKSSPLSSVSSVDHEEQTLRQ
DSLSSDKNPMTMKKPKLNKRVLPSKPKKSVKENLDELSRRNIDVMHLQHILTNHLAFA
NVQQTPLFQLQQVNSQISELSRDELRSILSDAKCVGVIYRHGKDAAGKPLDEEYFYDL
ENDDDYERRNLVSSLKGGRTGLRSCRRTHKQYFWKKPAK"
gene 472546..473171
/gene="Rpl37a"
/locus_tag="R008_L11516"
mRNA join(472546..472552,472912..473171)
/gene="Rpl37a"
/locus_tag="R008_L11516"
/product="Rpl37ap"
CDS join(472546..472552,472912..473171)
/gene="Rpl37a"
/locus_tag="R008_L11516"
/note="Similar to YLR185W Protein component of the large
(60S) ribosomal subunit, has similarity to Rpl37Bp and to
rat L37 ribosomal protein"
/codon_start=1
/product="Rpl37ap"
/protein_id="EWG84430.1"
/translation="MGKGTPSFGKRHNKSHTLCNRCGRRSFHVQKKTCSSCGYPAAKT
RSYNWGAKAKRRHTTGTGRMRYLKHVSRRFKNGFQTGSASKASA"
gene 473515..474273
/gene="Emg1"
/locus_tag="R008_L11521"
mRNA 473515..474273
/gene="Emg1"
/locus_tag="R008_L11521"
/product="Emg1p"
CDS 473515..474273
/gene="Emg1"
/locus_tag="R008_L11521"
/note="Similar to YLR186W 'Member of the alpha/beta knot
fold methyltransferase superfamily; required for
maturation of 18S rRNA and for 40S ribosome production;
interacts with RNA and with S-adenosylmethionine;
associates with spindle/microtubules; forms homodimers'"
/codon_start=1
/product="Emg1p"
/protein_id="EWG84431.1"
/translation="MVEDSRVRDALKGGDQKALPASLVPQAPPVLTSKDKITKRMIVV
LAMASLETHKISSNGPGGDKYVLLNCDDHQGLLKKMGRDISEARPDITHQCLLTLLDS
PINKAGKLQVYIQTSRGILIEVNPTVRIPRTFKRFSGLMVQLLHKLSIRSVNSEEKLL
KVIKNPITDHLPTKCRKVTLSFDAPVIRVQDYIEKLDDDESICVFVGAMARGKDNFAD
EYVDEKVGLSNYPLSASVACSKFCHGAEDAWNIL"
gene 474748..477828
/gene="Skg3"
/locus_tag="R008_L11526"
mRNA 474748..477828
/gene="Skg3"
/locus_tag="R008_L11526"
/product="Skg3p"
CDS 474748..477828
/gene="Skg3"
/locus_tag="R008_L11526"
/note="Similar to YLR187W 'Protein of unknown function;
green fluorescent protein (GFP)-fusion protein localizes
to the cell periphery, cytoplasm, bud, and bud neck;
potential Cdc28p substrate; similar to Caf120p and Skg4p'"
/codon_start=1
/product="Skg3p"
/protein_id="EWG84432.1"
/translation="MKRIFSGVKSPKLSAPPKVFKNDESPSTPSSPKFDQGLRSLSAS
ASRLFSNSISTPGSPTLDLPQEHSINGDISPELVPIVTLLSAQAHRRYHYGIFLILHD
LKTDGTPAVRQWEECYGVLLGTQLALWDAKELSDSKNNKNTSTMKKAASRPSFINFTD
ASVRSLDANDQVISASENEKTKKDLDNVLVVSTTLKNRYFLKFKNSKSFKTWNAAIRL
SLFEFTALQEAYTGSFLSSRGVKLGDIKVVMADTKFTYEDWVSVRFGTGMPWKRCYAV
ISPQSGKKKKNSKGSICFYENNKKTKKSNIMTTVVDARALYAVYPSSPILIDTSTIIK
LEGFVSFDKSEEPQETNLFIMPEKHQGVPGYDTIIRFLIPAMNAFYLYGRPKGLIANR
TDPDSLLFALPTLPHIYYLQVDDVLSLTKDKNYIHWSAADWRNNIVQVLQKKLSKGYK
GCGNKSVSVSSGMMKSPAISSAELFEGYDSLPERQMESPQKSKMKSPTLASTDDINSA
SASVNSHAISVKQTELFVTDNSSKINDSVSAQSSVTTNFKDTFTTPMTSGMLNQENSE
RSFGSGLKLKITDSNLENMEDVEAKSANEFSTTPEDKHIHLANAAELSALYDKYSTSP
FGKSEANSSPKPQKLEVKDRSKNENRSPYERYVGTSAESKTFEIGNVRESKSTINTSL
SSPLRVEDSRRSKNEDLGSLKEFEELSQKISNMGMANISSEALSDTAENSSFVTDLNL
NINNSSSVNLNEEQRVPDFGEENVFDPDYMEQNQMLETESRYTTDEFDFSDNQDAASS
NYSNGQTNRTVTETLSASDRNDKIPHSSLFTNLNQLTSNGGNYQDREDFSGDQINKPQ
QSQPLHVKGPQTSSFGYRNSSANSSQPQAPYPVGRPLGKIRTGPLTVQPIQQGGDSSM
YSFQSSQHRFHSSQQRQNQSLSFRNNTYGSGNNQNTLHPSPQLQQQPQNMRYLNNKLP
INDRSPIPQTQHHVPDGRPSLHINTTNRTNPLTAQSGFSQFMPPNSTSTNPYSS"
gene 478179..480266
/gene="Mdl1"
/locus_tag="R008_L11531"
mRNA 478179..480266
/gene="Mdl1"
/locus_tag="R008_L11531"
/product="Mdl1p"
CDS 478179..480266
/gene="Mdl1"
/locus_tag="R008_L11531"
/note="Similar to YLR188W Mitochondrial inner membrane
half-type ATP-binding cassette (ABC) transporter, mediates
export of peptides generated upon proteolysis of
mitochondrial proteins, plays a role in the regulation of
cellular resistance to oxidative stress"
/codon_start=1
/product="Mdl1p"
/protein_id="EWG84433.1"
/translation="MIVRMIRLCKGPKLLRSQFASASALYSTKSLFKPPMYQKAEINL
IIPHRKHFLLRSIRLQSDIAQGKKSTKPTLKLSNANSKSSGFKDIKRLFVLSKPESKY
IGLALLLILISSSVSMAVPSVIGKLLDLASESDGEDEEGSKSNKLYGLTKKQFFTALG
AVFIIGAVANASRIIILKVTGERLVARLRTRTMKAALDQDATFLDTNRVGDLISRLSS
DASIVAKSVTQNVSDGTRAIIQGFVGFGMMSFLSWKLTCVMMILAPPLGAMALIYGRK
IRNLSRQLQTSVGGLTKVAEEQLNATRTIQAYGGEKNEVRRYAKEVRNVFHIGLKEAV
TSGLFFGSTGLVGNTAMLSLLLVGTSMIQSGSMTVGELSSFMMYAVYTGSSLFGLSSF
YSELMKGAGAAARVFELNDRKPLIRPTIGKDPVSLAQKPIVFKNVSFTYPTRPKHQIF
KDLNITIKPGEHVCAVGPSGSGKSTIASLLLRYYDVNSGSIEFGDEDIRNFNLRKYRR
LIGYVQQEPLLFNGTILDNILYCIPPEIAEQDDRIRRAIGKANCTKFLANFPDGLQTM
VGARGAQLSGGQKQRIALARAFLLDPAVLILDEATSALDSQSEEIVAKNLQRRVERGF
TTISIAHRLSTIKHSTRVIVLGKHGSVVETGSFRDLIAIPNSELNALLAEQQDEEGKG
GGIDLDNSVAQEV"
gene complement(480675..484271)
/gene="Atg26"
/locus_tag="R008_L11536"
mRNA complement(480675..484271)
/gene="Atg26"
/locus_tag="R008_L11536"
/product="Atg26p"
CDS complement(480675..484271)
/gene="Atg26"
/locus_tag="R008_L11536"
/note="Similar to YLR189C 'UDP-glucose:sterol
glucosyltransferase, conserved enzyme involved in
synthesis of sterol glucoside membrane lipids; in contrast
to ATG26 from P. pastoris, S. cerevisiae ATG26 is not
involved in autophagy'"
/codon_start=1
/product="Atg26p"
/protein_id="EWG84434.1"
/translation="MPITQIISASDSEAGPKPSISLVPDKPSEPETSPRHHRLSRSLS
KFKRWRGRSNSSLSMGSSEQQELQDSPNEARSDDDENGYNNDNADDLAKSKYMMKSIA
GLLTTASVYAGMNNAQEMNVLSQVDSEESDSSDSFQENIGRNEVKSKKENLKTKSHPE
VPRLDKRKPTLFDFSITREKLSKDNVAKLRQRFCLDEREPFLNDFPAWLLKDVLVQGH
IFITTKHFLFFAYLPKNPRSVKMSGNLNIRTKLIRSTRYWCVLKNHLFSMYTSSTELY
FPVLTIDLREVQKIETQKHTLNGSATKTFKLYTDESTFKFNADSEFSAKSWVNALKKE
QFAAQNSENNSISLKIPLPNIIEIDDQPIVNKALTLRLRALESSQTYAIDDFMFVFMD
GSGSQVKESLGEQLAILQKSGVNTLYYDIPAKKSKSSFGKETPATAEQKNNGEDSKYL
NVPTSAVPSSENGKKSRFRFRERSNSWFRRAKPLEDSQVEDVEEIYKDAANDIDSSVH
STIHIHEQEDSQEQTVAWKPSHLKNFAEMWAAKPIHYRNKFIPFQKDDTYLIKETEEV
SANERFRYHFKFNKEKSLISTYYTYLNRNVPVYGKIYVSNDTVCFRSLLPGSNTYMVL
PLVDVETCYKEKGFRFGYFVLVIVIHGHEELFFEFSTEVARDDIERILLKLLDNIYAS
SAEGSNISSASLGDVQHNPDSAKLKLFEDKINAEGFEVPLMIDENPHYKTSIKPNKSY
KFGLLTIGSRGDVQPYIALGKGLIKEGHQVVIITHSEFRDFVESHGIQFEEIAGNPVE
LMSLMVENESMNVKMLREASSKFRGWIDALLQTSWEVCNRRKFDILIESPSAMVGIHI
TEALQIPYFRAFTMPWTRTRAYPHAFIVPDQKRGGNYNYLTHVLFENVFWKGISGQVN
KWRVETLGLGKTNLFLLQQNNVPFLYNVSPTIFPPSIDFSEWVRVTGYWFLDDKSTFK
PPAELQEFISEARSKGKKLVYIGFGSIVVSNAKEMTEALVEAVMEADVYCILNKGWSE
RLGDKAAKKTEVDLPRNILNIGNVPHDWLFPQVDAAVHHGGSGTTGASLRAGLPTVIK
PFFGDQFFYAGRVEDIGVGIALKKLNAQTLADALKVATTNKIMKDRAGLIKKKISKED
GIKTAISAIYNELEYARSVTLSRVKTPRKKEENVDATKLTPAETTDEGWTMI"
gene 485091..486566
/gene="Mmr1"
/locus_tag="R008_L11541"
mRNA 485091..486566
/gene="Mmr1"
/locus_tag="R008_L11541"
/product="Mmr1p"
CDS 485091..486566
/gene="Mmr1"
/locus_tag="R008_L11541"
/note="Similar to YLR190W 'Phosphorylated protein of the
mitochondrial outer membrane, localizes only to
mitochondria of the bud; interacts with Myo2p to mediate
mitochondrial distribution to buds; mRNA is targeted to
the bud via the transport system involving She2p'"
/codon_start=1
/product="Mmr1p"
/protein_id="EWG84435.1"
/translation="MNSPTMKSEQLTPKLSPMSFCLDDQRNAGSFQNLLNSPTKLKLD
TGPIGNSLLYPTSLSKLSELSRGGRSKQRRGSDTMRSVSPIRFQFLNNTPKMLKPEYL
SQTTSNLPLLSALLKNSKKTTSEGQNSNPDPLNIEKNIIKQSIKDKLEQLRSSESVAQ
VQKKERNPPSFEAKVCAEEPILRKNAEGLLPSYVPVPATPLEDPENHGVRKVEDKGLR
VVSGGSTQCLSTEVNELPKDLNLDNLPTDNNGFVQYGLKGNNNNNRYSFISSTSTDYE
PEWCDGQQHISMQMASMANAEEANSREKSNLDIKIKQLELEITELKLQNEKLVHSMTT
NRYIEERFMLEVMKDPSIQAQRSQRDIERKVKQLEKKFFNCKKVLKKLTESSAVVATS
TSKTEGNSARIPCPKTRLARVSVLDLKKIEEQPDSSSGTSSEEDHLTNDDTDANTSED
LNVAFEEEPTSAISTTASVQSGESKRGFQLNLPVQVEKKEK"
gene 487148..488308
/gene="Pex13"
/locus_tag="R008_L11546"
mRNA 487148..488308
/gene="Pex13"
/locus_tag="R008_L11546"
/product="Pex13p"
CDS 487148..488308
/gene="Pex13"
/locus_tag="R008_L11546"
/note="Similar to YLR191W Integral peroxisomal membrane
protein required for translocation of peroxisomal matrix
proteins, interacts with the PTS1 signal recognition
factor Pex5p and the PTS2 signal recognition factor Pex7p,
forms a complex with Pex14p and Pex17p"
/codon_start=1
/product="Pex13p"
/protein_id="EWG84436.1"
/translation="MSSTAVPRPKPWETSASLEEPQRNAQSLSAMMTSNQQDSRPTEE
SNNSNSASESAPEVLPRPAALNSSGTYGESNTIPGIYGNSNYGIPYDNNPYSMNSIYG
NSIGRYGYGGSYYGNNYGSFYGGGYGAGAGYGMNNGSGLGESTKATFQLIESLIGAVT
GFAQMLESTYMATHNSFFTMISVAEQFGNLKEMLGSFFGIFAIMKFLKKILYRATKGR
LGIPPKNFAESEGSKNKLIEDFQKFNDSGTINSNEKATRRKISWKPLLFFLMAVFGFP
YLLNKFITKLQTSGTIRASQGNGSEPIDPSKLEFARALYDFVPENPEMEVALKKGDLM
AILSKKDPLGRDSDWWKVRTKNGNIGYIPYNYIEIIKRRKKIEHVDDETRTH"
gene complement(488668..489462)
/gene="Hcr1"
/locus_tag="R008_L11551"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(489882..490409)
/gene="Ups1"
/locus_tag="R008_L11556"
mRNA complement(489882..490409)
/gene="Ups1"
/locus_tag="R008_L11556"
/product="Ups1p"
CDS complement(489882..490409)
/gene="Ups1"
/locus_tag="R008_L11556"
/note="Similar to YLR193C 'Mitochondrial intermembrane
space protein that regulates mitochondrial cardiolipin
levels, null has defects in Mgm1p processing, integrity of
mitochondrial inner membrane complexes, and mitochondrial
morphology; ortholog of human PRELI'"
/codon_start=1
/product="Ups1p"
/protein_id="EWG84437.1"
/translation="MVLLHKSTHIFPTDFASVSRAFFNRYPNPYSPHVLSIDTISRNV
DQEGNLRTTRLLKKSGKLPTWVKPFLRGITETWIIEVSVVNPANSTMKTYTRNLDHTG
IMKVEEYTTYQFDSATSSTIADSRVKFSSGFNMGIKSKVEDWSRTKFDENVKKSRMGM
AFVIQKLEEARNPQF"
gene complement(490682..491446)
/locus_tag="R008_L11566"
mRNA complement(490682..491446)
/locus_tag="R008_L11566"
/product="hypothetical protein"
CDS complement(490682..491446)
/locus_tag="R008_L11566"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84438.1"
/translation="MKACSILFTTLITLAAAQKDSGSLDGQNSEDSSQKESSNSQEIT
PTTTKEAQESASTVVSTGKSLVQTSNVVSNTYAVAPSTTVVTTDAQGKTTTQYLWWVA
ESNSAVSTTSTASVQPTGETSSGITNSASSSTTSTSTDGPVTIVTTTNSLGETYTSTV
WWLPSSATTDNTASSSKSSSGSSSKPESSTKVVSTIKSTYTTTSGSTVETLTTTYKST
VNGKVASVMSNSTNGAFAGTHIAYGAGAFAVGALLL"
gene complement(491810..493177)
/gene="Nmt1"
/locus_tag="R008_L11571"
mRNA complement(491810..493177)
/gene="Nmt1"
/locus_tag="R008_L11571"
/product="Nmt1p"
CDS complement(491810..493177)
/gene="Nmt1"
/locus_tag="R008_L11571"
/note="Similar to YLR195C N-myristoyl transferase,
catalyzes the cotranslational, covalent attachment of
myristic acid to the N-terminal glycine residue of several
proteins involved in cellular growth and signal
transduction"
/codon_start=1
/product="Nmt1p"
/protein_id="EWG84439.1"
/translation="MSEEDKAKKLENLLKLLQLNNGDTSKFTQEQKKAMKDHKFWRTQ
PVKDFDEKVVEEGPIDKPKTPEDISDKPLPLLSSFEWCSIDVDNKKQLEDVFVLLNEN
YVEDRDAGFRFNYTKEFFNWALKSPGWKKDWHIGVRVKETQKLVAFISAIPVTLGVRG
KQVPSVEINFLCVHKQLRSKRLTPVLIKEITRRVNKCDIWHALYTAGIVLPAPVSTCR
YTHRPLNWKKLYEVDFTGLPDGHTEEDMIAENALPAKTKTAGLRKLKKEDIDQVFELF
KRYQSRFELIQIFTKEEFEHNFIGEESLPLDKQVIFSYVVEQPDGKITDFFSFYSLPF
TILNNTKYKDLGIGYLYYYATDADFQFKDRFDPKATKALKTRLCELIYDACILAKNAN
MDVFNALTSQDNTLFLDDLKFGPGDGFLNFYLFNYRAKPITGGLNPDNSNDIKRRSNV
GVVML"
gene 493862..495592
/gene="Pwp1"
/locus_tag="R008_L11581"
mRNA 493862..495592
/gene="Pwp1"
/locus_tag="R008_L11581"
/product="Pwp1p"
CDS 493862..495592
/gene="Pwp1"
/locus_tag="R008_L11581"
/note="Similar to YLR196W 'Protein with WD-40 repeats
involved in rRNA processing; associates with trans-acting
ribosome biogenesis factors; similar to beta-transducin
superfamily'"
/codon_start=1
/product="Pwp1p"
/protein_id="EWG84440.1"
/translation="MISATNWVPRGFSSEFPEKYVLDDEEVERINQLAQLNLDDAKAT
LEEAEGESGVEDDAATGSSNKLKDQLDIDDDLKEYNLEEYDDEEIADNEGGKDVSMFP
GLSNDSDVKFHEGEKGEDPYISLPNQEDSQEEKQELQVYPSDNLVLAARTEDDVSYLD
IYVYDDGAGFHSSDIPVEEGDEADPDVARGLVRDPALYVHHDLMLPAFPLCVEWLDYK
VGSNSEEAANYAAIGTFDPQIEIWNLDCVDKAFPDMILGEPLDNSMVSLKSKKKKKKS
KTGHITTHHTDAVLSMAHNKYFRSVLASTSADHTVKLWDLNSGNAARSLASIHSNKNV
SSSEWHMLNGSILLTGGYDSRVALTDVRISDESQMSKYWSAMAGEEIETVTFASENII
LCGTDSGNVYSFDIRNNENRKPVWTLKAHDAGISTLCSNKFIPGMMSTGAMGEKTVKL
WKFPLDDATNTKGPSMVLSRDFDVGNVLTSSFAPDIEVAGTMVIGGVNKVLKLWDVFT
NRSVRKSFKSELENVQARAKEEAQKLGKSSRIARKYTSNDNPDTVITIDDQGEDEEER
EGEDEHDDMA"
gene 495991..497505
/gene="Sik1"
/locus_tag="R008_L11586"
mRNA 495991..497505
/gene="Sik1"
/locus_tag="R008_L11586"
/product="Sik1p"
CDS 495991..497505
/gene="Sik1"
/locus_tag="R008_L11586"
/note="Similar to YLR197W 'Essential
evolutionarily-conserved nucleolar protein component of
the box C/D snoRNP complexes that direct 2'-O-methylation
of pre-rRNA during its maturation; overexpression causes
spindle orientation defects'"
/codon_start=1
/product="Sik1p"
/protein_id="EWG84441.1"
/translation="MAPIEYLLFEEPTGYAVFKVKLQQDDIGSRLKEVQEQINDFGAF
TKLIELVSFAPFKGAAEALENANDISEGLVSESLKAILDLNLPKASSKKKNITLAISD
KNLGPSIKEEFPYVDCISNELAQDLIRGVRLHGEKLFKGLQSGDLERAQLGLGHAYSR
AKVKFSVQKNDNHIIQAIALLDQLDKDINTFAMRVKEWYGWHFPELAKLVPDNYTFAK
LVLFIKDKASLNDDSLHDLAALLNEDSGIAQRVIDNARISMGQDISETDMENVCVFAQ
RVASLADYRRQLYDYLCEKMHTVAPNLSELIGEVIGARLISHAGSLTNLSKQAASTVQ
ILGAEKALFRALKTKGNTPKYGLIYHSGFISKASAKNKGRISRYLANKCSMASRIDNY
SEEPSNVFGSVLKKQVEQRLEFYNTGKPTLKNELAIQEAMELYNKDKPAAEVEETKEK
ESSKKRKLEDDDEEKKEKKEKKSKKEKKEKKEKKDKKEKKDKKEKKDKKKKSKD"
gene complement(497747..498663)
/gene="Pba1"
/locus_tag="R008_L11601"
mRNA complement(join(497747..498571,498658..498663))
/gene="Pba1"
/locus_tag="R008_L11601"
/product="Pba1p"
CDS complement(join(497747..498571,498658..498663))
/gene="Pba1"
/locus_tag="R008_L11601"
/note="Similar to YLR199C 'Protein involved in 20S
proteasome assembly; forms a heterodimer with Add66p that
binds to proteasome precursors; similar to human PAC1
constituent of the PAC1-PAC2 complex involved in
proteasome assembly'"
/codon_start=1
/product="Pba1p"
/protein_id="EWG84442.1"
/translation="MLFKQWNDLPEPKHLLDLPEISKNLQSLEVCPVPKVEFPQDLDV
SQYSTAVITTKIMNPLFPKNLLQLTSIGEIKTTLTVKSPSLPQSSGKHSWNYDENFPN
EVDPDQKNDTADETVYGFSFPIYSFGKTLLFSMEENFISISPIFGNMISRSIISQLAQ
FSPDIIVIGTSDKIASMKVMTENECTLQPPEFITGFIGSVLTQLIVGPSKGLKFKCLV
APSEGPNGFEKLSLSDMGSLVDLCGQWLGFEPSRYSEECYRLWRCDSAAIGAQSGLYI
"
gene 498906..499250
/gene="Yke2"
/locus_tag="R008_L11606"
mRNA 498906..499250
/gene="Yke2"
/locus_tag="R008_L11606"
/product="Yke2p"
CDS 498906..499250
/gene="Yke2"
/locus_tag="R008_L11606"
/note="Similar to YLR200W Subunit of the heterohexameric
Gim/prefoldin protein complex involved in the folding of
alpha-tubulin, beta-tubulin, and actin"
/codon_start=1
/product="Yke2p"
/protein_id="EWG84443.1"
/translation="MSELGAKYQQLQNELEEFIVARQKLETQLQENKIVNEEFDQLEE
DTPVYKLTGNVLLPVEQSEARTNVDKRLEFIETEITRCEKNIRDKQEELEKVRSELIK
LNNTAASTGPGR"
gene complement(499405..500187)
/gene="Coq9"
/locus_tag="R008_L11611"
mRNA complement(499405..500187)
/gene="Coq9"
/locus_tag="R008_L11611"
/product="Coq9p"
CDS complement(499405..500187)
/gene="Coq9"
/locus_tag="R008_L11611"
/note="Similar to YLR201C 'Protein required for ubiquinone
(coenzyme Q) biosynthesis and respiratory growth;
localizes to the matrix face of the mitochondrial inner
membrane in a large complex with ubiquinone biosynthetic
enzymes'"
/codon_start=1
/product="Coq9p"
/protein_id="EWG84444.1"
/translation="MLCRNTARTGCKFFRLYHSNPIEHVKPIHIKPLTYGKESPQYKV
LSLALQKFVPEHGFSERSIVESLNELGYPSSMISSIGAPNSPSFFHSSTAVMELIKFQ
LVDKRYRLTEGINPDVTPQYKLPSLEHLLLKRLEMDKPIGGHLSELMSQLAIPSAFLF
ETAIPELHRLSDDMIYFSNEKDHHDSAWYAKRLAVSSTYIGSKLFMAQDKSHNYKETF
TFAKDKLHRVMRLGEYYNNTEEFAWYTLMSTVNLIKSQLVRG"
gene complement(500543..501853)
/gene="Mss51"
/locus_tag="R008_L11621"
mRNA complement(500543..501853)
/gene="Mss51"
/locus_tag="R008_L11621"
/product="Mss51p"
CDS complement(500543..501853)
/gene="Mss51"
/locus_tag="R008_L11621"
/note="Similar to YLR203C 'Specific translational
activator for the mitochondrial COX1 mRNA; loosely
associated with the matrix face of the mitochondrial inner
membrane; influences both COX1 mRNA translation and Cox1p
assembly into cytochrome c oxidase'"
/codon_start=1
/product="Mss51p"
/protein_id="EWG84445.1"
/translation="MTVLYAPSGATQLYFHLLRKSPHNRLVVSHQTRRHLMGFVRNAL
GLDPPPSPEDPTPENRFHPWDQSPSVDLRERAAKIRTLAHCPVTGKDINYTCPLSGIP
THHSREAWEMDKAYHDSKKYEILKKVNIYEHDLRSGRPFPEFDFPQQQGYDKAVNLTN
WDLFFYTRSFYSMDTEFQLAAVTKMLSYPITIGSLLHKFSPYSLNPKGPITLEGLKSL
AALRYTLYPLENRSLPTTTKNRAMRIFILGARAEAQLPGHVWKQLQFLFPEQSFEIHF
IGPECLYKRDKQEYVKSTTPVVQRVDETLKFIYRTNFFEVFHEAQDFFPYDPYMDVFF
TFHPGYASPESHGSWMGETMKALLETKCAIFTTGFNKKDLTDDINLVKSKYGKEMDVL
MEPVRNVFGSTKWELNDMNPQEVYQFNMYIAGFRGKRYHTIKRQ"
gene 502163..502498
/gene="Qri5"
/locus_tag="R008_L11626"
mRNA 502163..502498
/gene="Qri5"
/locus_tag="R008_L11626"
/product="Qri5p"
CDS 502163..502498
/gene="Qri5"
/locus_tag="R008_L11626"
/note="Similar to YLR204W 'Mitochondrial inner membrane
protein, required for accumulation of spliced COX1 mRNA;
may have an additional role in translation of COX1 mRNA'"
/codon_start=1
/product="Qri5p"
/protein_id="EWG84446.1"
/translation="MLGRALRPGWLGITRTVVKKPSCGSYFNRTFQTAINTTMPPMQE
GMLSTMMMMTATATRITGTVSEPLNGSNIVMQLDSVMRKRKKKMKKHKLRKRRKREKA
ERRKLSQGR"
gene complement(502615..503571)
/gene="Hmx1"
/locus_tag="R008_L11631"
mRNA complement(502615..503571)
/gene="Hmx1"
/locus_tag="R008_L11631"
/product="Hmx1p"
CDS complement(502615..503571)
/gene="Hmx1"
/locus_tag="R008_L11631"
/note="Similar to YLR205C 'ER localized heme oxygenase,
involved in heme degradation during iron starvation and in
the oxidative stress response; expression is regulated by
AFT1 and oxidative stress; relocates to the perinuclear
region in the presence of oxidants'"
/codon_start=1
/product="Hmx1p"
/protein_id="EWG84447.1"
/translation="MEDSNNTNIPSPTDVGALANRINFQTRDAHNKINTFMGIKMAIA
MRHGFIYRQGILAYYYVFDAIEQEIDRLLNDPVTEEELQTSTILKQFWLEDFRRSTQI
YKDLKLLYSNTFKSTESLNEFLATFQKPPLLQQFINNIHENIHKEPCTILSYCHVLYL
ALFAGGKLIRSNLYRRLGLFPNFEKLSQKELVKKGTNFFTFSVLGPTEETRLKWEYKK
NYELATRTELTEAQKLQIISVAEGIFDWNFNIVAEIGELNRRELMGKFSFKCITYLYE
EWMFNKDSATRRALHTVMLLVLSIIAIWVLYFLVKKSFLSIV"
gene 504474..506324
/gene="Ent2"
/locus_tag="R008_L11641"
mRNA 504474..506324
/gene="Ent2"
/locus_tag="R008_L11641"
/product="Ent2p"
CDS 504474..506324
/gene="Ent2"
/locus_tag="R008_L11641"
/note="Similar to YLR206W 'Epsin-like protein required for
endocytosis and actin patch assembly and functionally
redundant with Ent1p; contains clathrin-binding motif at
C-terminus'"
/codon_start=1
/product="Ent2p"
/protein_id="EWG84448.1"
/translation="MSKQFVRSAKNMMKGYSSTQVLVRDATANDSRTPSIDTLDDLAQ
RSYDSVDFFEIMDMLDKRLNDKGKYWRHVAKSLTVLDYLVRFGSENCVLWCRENFYVI
KTLREFRHENESGFDEGQIIRVKAKELVSLLNDEERLREERSMNTRNRRANRAARPRP
RRQRTRSNPHDSSPSYQDDLEKALEESRITAQEDEQRRRELAQYDDEDPDFQAALQLS
KEEEELKQLQELQRLQKQQQSLSQFQAPLQQQPQQQPAYYDIFGNPISQDEYLQYQYQ
QDQEQAMAQQRWLDQQQEQQQLAEQQYFQQQQQAAAAASALQQQQTAANMQQQQQQQQ
PADFQQPLPTGSNNPFSMDNLERQKQEQQHAQLQRQQEEARQQQEQLKLQQLQRQQQE
EAQLHQKRQEEAQLQQQQAQLLQQQAQFQQQQPLKQTRTGNQSISDKYSDLNTLLATG
TGIDTFGNTGEARIPAQHTKTGTFINSQGTGYKQVTNEPKNNPFLSNQYTGLPSTNIV
PTQTGYGFGNQPQSPPTNSPQQNPTGISYSQPQQQQQPQQQPQYMQNFQQQQPQYAQN
FQQQPQYTQNYQQQPQYIQPHQQQQQQQQQQQQQQQGYTPDQGVSLIDLN"
assembly_gap 506410..506520
/estimated_length=111
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 506703..509219
/gene="Hrd3"
/locus_tag="R008_L11646"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 509481..510374
/gene="Sec13"
/locus_tag="R008_L11651"
mRNA 509481..510374
/gene="Sec13"
/locus_tag="R008_L11651"
/product="Sec13p"
CDS 509481..510374
/gene="Sec13"
/locus_tag="R008_L11651"
/note="Similar to YLR208W 'Component of the Nup84 nuclear
pore sub-complex, the Sec13p-Sec31p complex of the COPII
vesicle coat, and the SEA (Seh1-associated) complex;
required for vesicle formation in ER to Golgi transport
and nuclear pore complex organization'"
/codon_start=1
/product="Sec13p"
/protein_id="EWG84449.1"
/translation="MVVIANAHNELIHDAVLDYYGKRLATCSSDKTIKIFEVEGETHK
LIDTLTGHEGPVWRVDWAHPKFGTILASCSYDGKVLIWKEENGRWSQIAVHAVHSASV
NSVQWAPHEYGPLLLVASSDGKVSVVEFKENGTTSPIIIDAHAIGVNSASWAPATIEE
DGEHNGTKESRKFVTGGADNLVKIWKYNSDAQTYVLESTLEGHSDWVRDVAWSPTVLL
RSYLASVSQDRTCIIWTQDNEQGPWKKTLLKEEKFPDVLWRASWSLSGNVLALSGGDN
KVTLWKENLEGKWEPAGEVHQ"
gene complement(510727..511662)
/gene="Pnp1"
/locus_tag="R008_L11656"
mRNA complement(510727..511662)
/gene="Pnp1"
/locus_tag="R008_L11656"
/product="Pnp1p"
CDS complement(510727..511662)
/gene="Pnp1"
/locus_tag="R008_L11656"
/note="Similar to YLR209C 'Purine nucleoside
phosphorylase, specifically metabolizes inosine and
guanosine nucleosides; involved in the nicotinamide
riboside salvage pathway'"
/codon_start=1
/product="Pnp1p"
/protein_id="EWG84450.1"
/translation="MSDILNVSQQREAITKAAAYISAILEPHFKNTTNFEPPRTLIIC
GSGLGGISTKLSRDNPPPVTVPYQDIPGFKKSTVPGHSGTLMFGSMNGSPVVLMNGRL
HGYEGNTLFETTFPIRVLNHMSHVRNLIVTNAAGGINAKYQACDLMCIYDHLNIPGLA
GQHPLRGPNLDEDGPRFLALSDAYDLELRKLLFKKWKELKIQRPLHEGTYTFVSGPTF
ETRAESKMIRMLGGDAVGMSTVPEVIVARHCGWRVLALSLITNTCVVDSPASALDESP
VPLEKGKATHAEVLENGKIASNDVQNLIAAVMGEL"
gene 511938..513320
/gene="Clb4"
/locus_tag="R008_L11661"
mRNA 511938..513320
/gene="Clb4"
/locus_tag="R008_L11661"
/product="Clb4p"
CDS 511938..513320
/gene="Clb4"
/locus_tag="R008_L11661"
/note="Similar to YLR210W 'B-type cyclin involved in cell
cycle progression; activates Cdc28p to promote the G2/M
transition; may be involved in DNA replication and spindle
assembly; accumulates during S phase and G2, then targeted
for ubiquitin-mediated degradation'"
/codon_start=1
/product="Clb4p"
/protein_id="EWG84451.1"
/translation="MMLEGYTVQPPQSTLIGDIEIQDENANQEVKNVLYQGVQKGIKR
LEKRQRRVALGDVTSQKANKIHNAIHNKFHQTKNNFEIENIRSSALVKEQQRDVRDED
SDYFLIDSSEGSSTDDEQVNEDAIDDLLSRRVNDQQIQADEVYEDFDGEMQDVIEEDV
DSQIEPLSPINNDEIQTELDRAFEKYFRSVPNPLDDDTHDVVMVVEYASDIFYYLREL
EVKYRPNPYYMQNQVELTWPFRRTMIDWLVQLHFRFQLLPETLYLTINIVDRFLSKKT
VTLNRFQLVGVSALFIAAKFEEINCPTLDDLVYMLENTYTRDDIIRAEQYMIDTLEFE
IGWPGPMPFLRRISKADDYDFEPRTLAKYLLETTIVEPKLVAAAPSWLAAGAYFLSRT
ILGSNDWSLKHVFYSGYTSSQIIPLASLILENCKNASRRHHSIWKKYFDQKHYRCSQI
VEEWIVSTEA"
gene complement(513725..514464)
/locus_tag="R008_L11666"
mRNA complement(join(513725..514387,514447..514464))
/locus_tag="R008_L11666"
/product="hypothetical protein"
CDS complement(join(513725..514387,514447..514464))
/locus_tag="R008_L11666"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84452.1"
/translation="MSTLAEVYTIIEDAEQECRKGDFTNAKAKYQEAIEVLGPQNENL
SQNKLSSDVTQAIDLLKQDITAKIQELELLIEKQSSEENNIGMVNNNMLIGSVILNNK
SPINGISNARNWDNPAYQDTLSPINDPLLMSILNRLQFNLNNDIQLKTEGGKNSKNSE
MKINLRLEQFKKELVLYEQKKFKEYGMKIDEITKENKKLANEIGRLRERWDSLVESAK
QRRDKQKN"
gene complement(514793..516214)
/gene="Tub4"
/locus_tag="R008_L11671"
mRNA complement(514793..516214)
/gene="Tub4"
/locus_tag="R008_L11671"
/product="Tub4p"
CDS complement(514793..516214)
/gene="Tub4"
/locus_tag="R008_L11671"
/note="Similar to YLR212C Gamma-tubulin, involved in
nucleating microtubules from both the cytoplasmic and
nuclear faces of the spindle pole body"
/codon_start=1
/product="Tub4p"
/protein_id="EWG84453.1"
/translation="MGGEIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSST
ERDDDTKPFFRENSRNKFTPRAIMMDSEPSVIADVENTFRGFFDPRNTWVASDGASAG
NSWANGYDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHSVAGGTGSGLGSNLLEALCD
RYPKKILTTYSVFPARSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFR
NPNIDLQHTNQLISTIISSVTNSIRFPSYMYSSMSSIYSTLIPSPELHFLSPSFTPFT
SDYIHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRA
MTKLQQRIKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACN
TFDKVFAKGAFLNNYNVGDLFQSMQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVL
VDDENMVGELEEDLDADGDHKLV"
gene complement(516587..517855)
/gene="Crr1"
/locus_tag="R008_L11681"
mRNA complement(516587..517855)
/gene="Crr1"
/locus_tag="R008_L11681"
/product="Crr1p"
CDS complement(516587..517855)
/gene="Crr1"
/locus_tag="R008_L11681"
/note="Similar to YLR213C 'Putative glycoside hydrolase of
the spore wall envelope; required for normal spore wall
assembly, possibly for cross-linking between the glucan
and chitosan layers; expressed during sporulation'"
/codon_start=1
/product="Crr1p"
/protein_id="EWG84454.1"
/translation="MRISILQLVPVIGYIGFALGELYKPKNSISCSPNNPCPAEWPCC
SPYNECGAGPICVGGCNVRSSFDEESCAPIPALVASQKLEFVSTPKVPKFIVNYQPKP
PIREGNGPNKANTKVGVVEGELNSKRIIHYAKFLVTPDSKEAEKMLEDFDFTHSGYTS
IEASSGNIVLAMPKKTTGSLITSTRSFLYGKASVRMKTARSRGVVTAFDLTSAIGDEI
DFEWLGGDLMTAQSNYYSQGHLDYTRMQRFPVGADTWATYHTYEIDWDPDRIIWYVDG
KIARTVLKKDTWDPISKEYRYPQTPMRLEIAVWPGGSETNGPGTINWAGGLIDWENSP
DIIEKGQFTAHVEQITVTPYQNKFTEQVQFCIKAKKKAPTFSQKDLSRVVVSYNRQDR
LNHHDEGSLKWDCFVTPKINDWLSSWKRSK"
gene 518498..520558
/gene="Fre1"
/locus_tag="R008_L11686"
mRNA 518498..520558
/gene="Fre1"
/locus_tag="R008_L11686"
/product="Fre1p"
CDS 518498..520558
/gene="Fre1"
/locus_tag="R008_L11686"
/note="Similar to YLR214W 'Ferric reductase and cupric
reductase, reduces siderophore-bound iron and oxidized
copper prior to uptake by transporters; expression induced
by low copper and iron levels'"
/codon_start=1
/product="Fre1p"
/protein_id="EWG84455.1"
/translation="MVRTRVLFCLFISFFATVQSSATLISTSCISQAALYQFGCSSKS
KSCYCKNINWLGSVTACAYENSKSNKTLDSALMKLASQCSSIKVYTLEDMKNIYLNAS
NYLRAPEKSDKKTVVSQPLMANETAYHYYYEENYGIHLNLMRSQWCAWGLVFFWVAVL
TAATILNILKRVFGKNIMANSVKKSLIYPSVYKDYNERTFYLWKRLPFNFTTRGKGLV
VLIFVILTILSLSFGHNIKLPHPYDRPRWRRSMAFVSRRADLMAIALFPVVYLFGIRN
NPFIPITGLSFSTFNFYHKWSAYVCFMLAVVHSIVMTASGVKRGVFQSLVRKFYFRWG
IVATILMSIIIFQSEKVFRNRGYEIFLLIHKAMNIMFIIAMYYHCHTLGWMGWIWSMA
GILCFDRFCRIVRIIMNGGLKTATLSTTDDSNVIKISVKKPKFFKYQVGAFAYMYFLS
PKSAWFYSFQSHPFTVLSERHRDPNNPDQLTMYVKANKDITRVLLSKVLSAPNHTVDC
KIFLEGPYGVTVPHIAKLKRNLVGVAAGLGVAAIYPHFVECLRLPSTDQLQHKFYWIV
NDLSHLKWFENELQWLKEKSCEVSVIYTGSSVEDTNSDESTKGFDDKEESEITVACLN
KRPDLKELVRSEIKLSELENNNITFYSCGPATFNDDFRNAVVQGIDSSLKIDVELEEE
SFTW"
gene complement(520707..521789)
/gene="Cdc123"
/locus_tag="R008_L11691"
mRNA complement(520707..521789)
/gene="Cdc123"
/locus_tag="R008_L11691"
/product="Cdc123p"
CDS complement(520707..521789)
/gene="Cdc123"
/locus_tag="R008_L11691"
/note="Similar to YLR215C 'Protein involved in nutritional
control of the cell cycle; regulates abundance of the
translation initiation factor eIF2; ortholog of human D123
protein'"
/codon_start=1
/product="Cdc123p"
/protein_id="EWG84456.1"
/translation="MSSQEYTTFIDIPVTREQVEHCSYSFWSSLYPKYVPKSIVLKSL
PKKFIQYLEQDGIKLPQEENSRSVYTEEIIRNEDNDYSDWEDDEDTATEFVQEVEPLI
DFPELHQKLKDALNELGAVAPKLNWSAPRDATWILPNNTMKCNEVNELYLLLNASNYI
MHDLQRAFKGCVDGDDIKGLKFDLVLRQWCDMNPALEFRVFVKNAHIVGATQRDLNYY
DYLDELSDTFKDLIDEIVHDVVLPKFPDKSFVLDVYIPRPFNKIFIVDINPFARKTDS
LLFSWNEIAAIAPPKNDVEDYELRLVTRHNTGRFASKEHSENHVPQDLVEASLNPEAI
RELTQKWKELLSQQAKEESSDSESET"
gene complement(522025..523140)
/gene="Cpr6"
/locus_tag="R008_L11696"
mRNA complement(522025..523140)
/gene="Cpr6"
/locus_tag="R008_L11696"
/product="Cpr6p"
CDS complement(522025..523140)
/gene="Cpr6"
/locus_tag="R008_L11696"
/note="Similar to YLR216C 'Peptidyl-prolyl cis-trans
isomerase (cyclophilin), catalyzes the cis-trans
isomerization of peptide bonds N-terminal to proline
residues; binds to Hsp82p and contributes to chaperone
activity'"
/codon_start=1
/product="Cpr6p"
/protein_id="EWG84457.1"
/translation="MTRPKTFFDISIGGKPQGRIVFELYNDIVPKTAENFLKLCEGNA
GMAKTKPDVPLSYKGSIFHRVIKDFMCQFGDFTNFNGTGGESIYGEKFEDENFTVKHD
KPFLLSMANAGPNTNGSQAFITCVPTPHLDGKHVVFGEVIQGKRIVRLIENQQCDQEN
NKPLRDVKIDDCGVLPDDYQVPENAEATPTDEYGDNYEDVLKQDEKVDLKNFDTVLKA
IETVKNIGTEQFKKQNYSVALEKYVKCDKFLKEYFPEDLEKEQIEKINQLKVSIPLNI
AICALKLKDYKQVLVASSEVLYAEAADEKAKAKALYRRGLAYYHVNDTDMALNDLEMA
TTFQPNDAAILKAIHDTKLKRKQQNEKAKKSLSKMFS"
gene complement(523395..523847)
/locus_tag="R008_L11706"
mRNA complement(523395..523847)
/locus_tag="R008_L11706"
/product="hypothetical protein"
CDS complement(523395..523847)
/locus_tag="R008_L11706"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84458.1"
/translation="MLLFSSFFSHNKCPKQKKGPQPLKVVKGTSKSCAKQGKIKGKEK
LWQAKTGRLVMSETGETSEYYKQALEEYKEVQEDEDPDVWDTRISKTGCYVENLALQL
CHAETGDWRQCFNEMALFRKCWEKNGNRERVSTVDVDGTTSKDSEKKK"
gene 524080..526266
/gene="Msc3"
/locus_tag="R008_L11711"
mRNA 524080..526266
/gene="Msc3"
/locus_tag="R008_L11711"
/product="Msc3p"
CDS 524080..526266
/gene="Msc3"
/locus_tag="R008_L11711"
/note="Similar to YLR219W 'Protein of unknown function,
green fluorescent protein (GFP)-fusion protein localizes
to the cell periphery; msc3 mutants are defective in
directing meiotic recombination events to homologous
chromatids; potential Cdc28p substrate'"
/codon_start=1
/product="Msc3p"
/protein_id="EWG84459.1"
/translation="MVFGFTKRDRRVPDLSRYDYYYQNHEDYNKSPQLSAAAASAASA
ASPDRTNYSRSHSLVSHAPSIPRQRSSVKSPGRRLSTSSAAPPTSRAAAKQYSQKTYS
LRSQRSGEYHLHPPGYTTNGSRMNSMTSGANVRRNYGKNKSTARNNNDSRANSITVKT
TQVTDPSGRTQSITKKTIRKINGYEYVETTTTTKNLVPLGDSQRHFDEFSENYMLQDD
DILEEQASDNIHDIIEENETDNEKPYSPVSESHLQDDSELNVEKPDFPPGSYFHHKYS
TDVMPLEEESSLSNFSDALDYIPPTHQTSSKYIHNKRKQASTTRRKKRPPAVKNAEAE
AKKPLTEAEMYLKALEVAKRNVYHTDAASDNASAPLGSNKSRKSRMGQKMTLRSSSDS
PTATANLVKSNVEVQPKRFTSSFFSRNTKSAPHEVHNHSVSTHFKSNKAVDPVPEPKS
ANTGLTDKEMYDQALKIAQARYYNSHGIQPEAVDNSTTAAKPRQVGVSHLGSTGSIPP
NEQHYLGDSEIPVQSEVHEYEPIPLQKTKTTGSSKNKFKTMFDKVLQFSQENYGYQHK
KEQGEQTPVTRNAEESFPAASISEGVTTAKPSSNEGVMTNPVVTDSPSPLQQQIDSTT
ASSNGQSQGNVPTSAVASTTRTRSPELQDNLKSSSSLLQDQTPQRQEDATDPTTSSTN
ELSAAEPTMVTSTHATKTIQAQTQDPPTKHKKSSFFTKLFKKKSSR"
gene 526755..527723
/gene="Ccc1"
/locus_tag="R008_L11716"
mRNA 526755..527723
/gene="Ccc1"
/locus_tag="R008_L11716"
/product="Ccc1p"
CDS 526755..527723
/gene="Ccc1"
/locus_tag="R008_L11716"
/note="Similar to YLR220W 'Putative vacuolar Fe2+/Mn2+
transporter; suppresses respiratory deficit of yfh1
mutants, which lack the ortholog of mammalian frataxin, by
preventing mitochondrial iron accumulation'"
/codon_start=1
/product="Ccc1p"
/protein_id="EWG84460.1"
/translation="MSIVALKNAVVTLIQKAKGSGGTSELGGSESTPLLRGSNSNSSR
HDNLSSSSSDIIYGRNSAQDLENSPMSVGKDNRNGDNGSDNEKANLGFFQSVDPRVIS
DLIIGLSDGLTVPFALTAGLSSLGDAKLVITGGFAELISGAISMGLGGYLGAKSESDY
YHAEVKKEKRKFYDNSNLINREIEDILLEINPNFSDETIVSFIKDLQRTPELMVDFII
RYGRGLDEPAENRELISAVTIGGGYLLGGLVPLVPYFFVSDVGTGLIYSIIVMVVTLF
WFGYVKTKLSMGSGSSTSKKVTEGVEMVVVGGVAAGAAWFFVKLLG"
gene complement(528292..528954)
/gene="Rsa3"
/locus_tag="R008_L11721"
mRNA complement(528292..528954)
/gene="Rsa3"
/locus_tag="R008_L11721"
/product="Rsa3p"
CDS complement(528292..528954)
/gene="Rsa3"
/locus_tag="R008_L11721"
/note="Similar to YLR221C 'Protein with a likely role in
ribosomal maturation, required for accumulation of
wild-type levels of large (60S) ribosomal subunits; binds
to the helicase Dbp6p in pre-60S ribosomal particles in
the nucleolus'"
/codon_start=1
/product="Rsa3p"
/protein_id="EWG84461.1"
/translation="MSAGDISAINIKSVKKNRRRKKRRTADVSSSDSSSSDPSSESEK
EEIQNGAIEEHVGENGKSDHVFSKGNDEDKQEDIAIEVSDVELTDEESKDLKLNSKEV
IDDLTKISLSKIPEPTKSQNKEGFMNASKIAENIKLAREEYNELAENFVPKGKDKTKL
REEYLNLLFENYGDDINRLRAAPDFTNKSLSILADALQEGIGMFDIGELELVLKNKEM
EN"
gene complement(529248..531701)
/gene="Utp13"
/locus_tag="R008_L11726"
mRNA complement(529248..531701)
/gene="Utp13"
/locus_tag="R008_L11726"
/product="Utp13p"
CDS complement(529248..531701)
/gene="Utp13"
/locus_tag="R008_L11726"
/note="Similar to YLR222C Nucleolar protein, component of
the small subunit (SSU) processome containing the U3
snoRNA that is involved in processing of pre-18S rRNA"
/codon_start=1
/product="Utp13p"
/protein_id="EWG84462.1"
/translation="MDLKTSYKGISLNPIYAGSSAVATVSENGKILATPVLDEINIID
LTPGSRKILHKISNEDEQEITALKLTPDGQYLTYVSQAQLLKIFHLKTGKVVRSMKIS
SPSYILDADSTSTLLAVGGTDGSIIVVDIENGYITHSFKGHGGTISSLKFYGQLNSKI
WLLASGDTNGMVKVWDLVKRKCLHTLQEHTSAVRGLDIIEVPDNDEPSLNLLSGGRDD
IINLWDFNMKKKCKLLKTLPVNQQVESCGFLKDGDGKRIIYTAGGDAIFQLIDSESGS
VLKRTNKPIEELFIIGVLPILSNSQMFLVLSDQTLQLINVEEDLKNDEDTIQVTSSIA
GNHGIIADMRYVGPELNKLALATNSPSLRIIPVPDLSGPEASLPLDVEIYEGHEDLLN
SLDATEDGLWIATASKDNTAIVWRYNENSCKFDIYAKYIGHSAAVTAVGLPNIVSKGY
PEFLLTASNDLTIKKWIIPKPTASMDVQIIKVSEYTRHAHEKDINALSVSPNDSIFAT
ASYDKTCKIWNLENGELEATLANHKRGLWDVSFCQYDKLLATSSGDKTVKIWSLDTFS
VMKTLEGHTNAVQRCSFINKQKQLISCGADGLIKIWDCSSGECLKTLDGHNNRLWALS
TMNDGDMIVSADADGVFQFWKDCTEQEIEEEQEKAKLQVEQEQSLQNYMSKGDWTNAF
LLAMTLDHPMRLFNVLKRALGESRSRQDTEEGKIEVIFNEELDQAISILNDEQLILLM
KRCRDWNTNAKTHTIAQRTIRCILMHHNIAKLSEIPGMVKIVDAIIPYTQRHFTRVDN
LVEQSYILDYALVEMDKLF"
gene complement(531936..532166)
/locus_tag="R008_L11731"
mRNA complement(531936..532166)
/locus_tag="R008_L11731"
/product="hypothetical protein"
CDS complement(531936..532166)
/locus_tag="R008_L11731"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84463.1"
/translation="MSYFAFFYGYYNQITFRLQYHHQYVTLPYSTLNFALSLVEKNYM
LFFYLYMHIFIEKHNQYFFYWYNPSNQTYKSK"
gene complement(532163..535420)
/gene="Ifh1"
/locus_tag="R008_L11736"
mRNA complement(532163..535420)
/gene="Ifh1"
/locus_tag="R008_L11736"
/product="Ifh1p"
CDS complement(532163..535420)
/gene="Ifh1"
/locus_tag="R008_L11736"
/note="Similar to YLR223C 'Coactivator that regulates
transcription of ribosomal protein (RP) genes; recruited
to RP gene promoters during optimal growth conditions via
Fhl1p; subunit of CURI, a complex that coordinates RP
production and pre-rRNA processing'"
/codon_start=1
/product="Ifh1p"
/protein_id="EWG84464.1"
/translation="MAGKKSPRKSTINHSTHSGKLPANIKRLIKKGESDTKSRQSPPT
LSTTRPRRFSLIYSSESSLSDVSDSDKNKSTNPHKIKRKAKNISNNSQGKKSKLIQRQ
IDNDDEGTESSDYQAVTDGEESENEEEESEEEEEDDDEDDDDDDDDGSDSDSDSETSS
DDENIDFVKLTAQRKKRAMKALSAMNTNSNTLYSSRENSNKNKSVKLSPKKENEEEQK
EEKEKEKEEQQKQQESNKKEVNGSGTTTTQQALSFKFKKEDDGISFGNGNEGYNEDIG
EEVLDLKNKENNGNEEDKLDSKVMLGNNDELRFPNISESDESEYDIDQDAYFDVINNE
DSHGEIGTDLETGEDDLPILEEEEQNIVSELQNDDELSFDGSIHEEGSDPVEDAENKF
LQNEYNQENGYDEEDDEEDEIMSDFDMPFYEDPKFANLYYYGDGSEPKLSLSTSLPLM
LNDEKLSKLKKKEAKKREQEERKQRRKLYKKTQKPSTRTTSNVDNDEYIFNVFFQSDD
ENSGHKSKKGRHKSGKSHIEHKNKGSNLIKSNDDLEPSTHSTVLNSGKYDSSDDEYDN
ILLDVAHMPSDDECSESETSHDADTDEELRALDSDSLDIGTELDDDYEDDDDDSSVTN
VFIDIDDLDPDSFYFHYDSDGSSSLISSNSDKENSDGSKDCKHDLLETVVYVDDESTD
EDDNLPPPSSRSKNIGSKAKEIVSSNVVGLRPPKLGTWETDNKPFSIIDGLSTKSLYA
LIQEHQQLREQHQRAQTPDVKREGSSNGNNGDELTLNELLNMSELEDDSPSHTDDMEN
NYNDAINSKSTNGHAADWYEVPKVPLSAFRNKGINAYEEDEYMIPANSNRKVPIGYIG
NERTRKKIDKMKELQRKKTEKKRQLKKKKKLLKIRKQRQKAIKEQETMNLQLGINGHE
IIGNNNSHSDINTGTDFTTNENTPMNELPSHAPEDASLIPHNSDLAVDSNTRKNSTKS
VGLDEIHEILGKDENDLLSVGDINGYDAQEGHVIEDTDADILASLTAPVQFDNTLSHE
NSNSMWRRRQSMVEAAAENLRFTKNGLFSESALADIEGIMGNDVNHSFEFNDVLQ"
gene 536394..537503
/locus_tag="R008_L11741"
mRNA 536394..537503
/locus_tag="R008_L11741"
/product="hypothetical protein"
CDS 536394..537503
/locus_tag="R008_L11741"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84465.1"
/translation="MNQSDSSLMDLPLEIHLSLLEYVPNELRAVNKYFYVLHNHSYKE
KSLAWIAEDNYIWAVVKHSLCLYVKSLDPLRQHAREIIQETKEPGFNVPLCMTKYIAD
SWYIVYNALQYPGKIINMGWDKYTKSQDSNGSDSTSNFNSRPKERTLMQSLTALPVNF
WSRRKDEPTPVNVWFYVKNAHVARYIPKIITEIGICNYGPKQIVASAGYINELITSEG
IYCVNLGHLPRLYDEQIFEGTGTTHLPLELKAIDRTDSDVCINGDLVLLGYDFIPYQI
SKPWLLFRIEPVNSIEAIFNYSECSFSYQFAWSLACLQSEEKISFPRDTIIGHGLPYK
PSKLIRIFVYKHPEQKQDLGQEIALPNWNTPYLRR"
gene complement(537625..538854)
/locus_tag="R008_L11746"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 539290..540475
/gene="Bur2"
/locus_tag="R008_L11751"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(540496..541977)
/gene="Ady4"
/locus_tag="R008_L11756"
mRNA complement(540496..541977)
/gene="Ady4"
/locus_tag="R008_L11756"
/product="Ady4p"
CDS complement(540496..541977)
/gene="Ady4"
/locus_tag="R008_L11756"
/note="Similar to YLR227C Structural component of the
meiotic outer plaque, which is a membrane-organizing
center that assembles on the cytoplasmic face of the
spindle pole body during meiosis II and triggers the
formation of the prospore membrane"
/codon_start=1
/product="Ady4p"
/protein_id="EWG84466.1"
/translation="MNKDVDYQTFKKSLRKEFKKAVKTILNLQEYNGDLIRDFLALYI
PYHVVFYNLSIMKKGSPLRIQTNNLLKEALAKILNFNLAMGPKHIIKIMKKDKADPET
MNKLKLVLYIKLFQGVFGHVDKNYNLAFQSFRWCLQFIAYSKRTRLFASIADEQIGDF
YELCELFISMLCCHCFLIDLKENEALVGNNLKKFIKRQNPNYSHGFDLNEETKSLQWH
WSLDEVDVIEALYCVAFDAMDKITLKFSKVNENFVFSQFFQYCAEIEEMLAILRGKIW
ECECDVFGPRIGLLVDSNHMNETIQKNILSITFKLKNDPQIICCLNKILEGLLLSSGV
QFKVIQFFYVLKLYYMQDNEYTFEASSEMDKLTIECLCIIENIIDACDNPDEVTDYQL
PKVLLTAMEGKLLVAEKISEDNDCSESLDDYHPRTYQFRHPRIIIDKMKTKLKQKLRF
DSPKDPETDDHWIEYWKYCYQDNIGNLPDILSRIYQTFTDPSN"
gene complement(542453..542553)
/locus_tag="R008_L11766"
tRNA complement(542453..542553)
/locus_tag="R008_L11766"
/product="tRNA-Leu"
/note="TL(UAG)L1"
assembly_gap 542669..549668
/estimated_length=7000
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(554862..555551)
/gene="Cdc42"
/locus_tag="R008_L11786"
mRNA complement(554862..555551)
/gene="Cdc42"
/locus_tag="R008_L11786"
/product="Cdc42p"
CDS complement(554862..555551)
/gene="Cdc42"
/locus_tag="R008_L11786"
/note="Similar to YLR229C 'Small rho-like GTPase,
essential for establishment and maintenance of cell
polarity; mutants have defects in the organization of
actin and septins'"
/codon_start=1
/product="Cdc42p"
/protein_id="EWG84467.1"
/translation="MQTLKCVVVGDGAVGKTCLLISYTTNQFPADYVPTVFDNYAVTV
MIGDEPYTLGLFDTAGQEDYDRLRPLSYPSTDVFLVCFSVISPPSFENVKEKWFPEVH
HHCPGVPCLVVGTQIDLRDDKVIIEKLQRQRLRPITSEQGSRLARELKAVKYVECSAL
TQRGLKNVFDEAIVAALEPPVIKKSIXXXXXXXXXXXXXXXXXXXXXXXXXXXXXFYY
RCITFFRFAQS"
assembly_gap 554909..554992
/estimated_length=84
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(556069..556201)
/locus_tag="R008_L11796"
tRNA complement(556069..556201)
/locus_tag="R008_L11796"
/product="tRNA-Ile"
/note="TI(UAU)L"
gene complement(556533..557894)
/gene="Bna5"
/locus_tag="R008_L11801"
mRNA complement(556533..557894)
/gene="Bna5"
/locus_tag="R008_L11801"
/product="Bna5p"
CDS complement(556533..557894)
/gene="Bna5"
/locus_tag="R008_L11801"
/note="Similar to YLR231C 'Kynureninase, required for the
de novo biosynthesis of NAD from tryptophan via
kynurenine; expression regulated by Hst1p'"
/codon_start=1
/product="Bna5p"
/protein_id="EWG84468.1"
/translation="MEKALELDGEYPESLRDEFNIPTFKSMGLSSDDKPVTYLCGNSL
GLMPKSTRNSINAELDAWSDCAVESHFKHPEEARGKVPWVSIDLPILPLLAPIVGAQE
NEVAVMNSLTANLNSLLITFYKPTEKRFKILFEKGSFPSDYYAFYNQCKIHGISEPEN
VFIQIEPREGETYIRTQDILDTIEVNQDELALVCLSGVQYYTGQYFDIGRITSFAHQF
PDILVGWDLAHAVGNVPLQLHDWGVDFACWCSYKYLNAGPGGIGGLFVHSKHTKPDPA
KESLPRLAGWWGNDPAKRFQMLEVFEPIPGALGFRQSNPSVIDTVALRSSLELFAKFN
GINEVRKRSLLLTNYMTELLEASKYYKHPLRIEKLPCFFTILTPTSTDEEHGAQLSLY
FDSDTGKEDIMPKVFQYLHDHGVIGDARRPNVIRLAPAPLYNTFSDVYIAVNALNEAM
DKL"
gene complement(558199..560297)
/gene="Est1"
/locus_tag="R008_L11816"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 560556..562526
/gene="Top3"
/locus_tag="R008_L11821"
mRNA 560556..562526
/gene="Top3"
/locus_tag="R008_L11821"
/product="Top3p"
CDS 560556..562526
/gene="Top3"
/locus_tag="R008_L11821"
/note="Similar to YLR234W DNA Topoisomerase III, conserved
protein that functions in a complex with Sgs1p and Rmi1p
to relax single-stranded negatively-supercoiled DNA
preferentially, involved in telomere stability and
regulation of mitotic recombination"
/codon_start=1
/product="Top3p"
/protein_id="EWG84469.1"
/translation="MKVLCVAEKNSIAKAVSQILGGGRSTSRDSGYMYVKNYDFMFSG
FPFARNGANCEVTMTSVAGHLTGIDFSHDSHGWGKCAIQELFDAPLNEIMNNNQKKIA
SNIKREARNADYLMIWTDCDREGEYIGWEIWQEAKRGNRLIQNDQVYRAVFSHLERQH
ILNAARNPSRLDMKSVHAVGTRIEIDLRAGVTFTRLLTETLRNKLRNQATMTKDGAKH
RGGNKNDSQVVSYGTCQFPTLGFVVDRFERIRNFVPEEFWYIQLVVENKDNGGTTTFQ
WDRGHLFDRLSVLTFYETCIETAGNVAQVVDLKSKPTTKYRPLPLTTVELQKNCARYL
RLNAKQSLDAAEKLYQKGFISYPRTETDTFPHAMDLKSLVEKQAQLDQLAAGGRTAWA
SYAASLLQPENTSNNNKFKFPRSGSHDDKAHPPIHPIVSLGPEANVSPVERRVYEYVA
RHFLACCSEDAKGQSTTLVLDWAVERFSASGLVVLERNFLDVYPWARWETTKQLPRLE
MNALVDIAKAEMKAGTTAPPKPMTESELILLMDTNGIGTDATIAEHIDKIQVRNYVRS
EKVGKETYLQPTTLGVSLVHGFEAIGLEDSFAKPFQRREMEQDLKKICEGHASKTDVV
KDIVEKYRKYWHKTNACKNTLLQVYDRVKASM"
gene 563136..564932
/gene="Thi7"
/locus_tag="R008_L11831"
mRNA 563136..564932
/gene="Thi7"
/locus_tag="R008_L11831"
/product="Thi7p"
CDS 563136..564932
/gene="Thi7"
/locus_tag="R008_L11831"
/note="Similar to YLR237W 'Plasma membrane transporter
responsible for the uptake of thiamine, member of the
major facilitator superfamily of transporters; mutation of
human ortholog causes thiamine-responsive megaloblastic
anemia'"
/codon_start=1
/product="Thi7p"
/protein_id="EWG84470.1"
/translation="MSFGSKVSRALRFLEIPVKDRASVSFLKNPDLQPIKSVNQTWGF
WSNFAYWGVMSFSVGTWMSASAALGVGLSYPETIGTFIVGDVLTIIFTLANSCPGYDW
KVGFTLAQRFVFGIYGSAFGIIIRILMSIVNYGSNAWLGGLCINMILDSWSHHYLHLP
NTLSSKVAMTTKELIGFIIFHVLTAFCYLMKPYHMNYILIWSCVATFFSMLGMVIYLA
KQAHGVGELFTSTKSTATGSTKAWAWVYMISYWFGSVSPGSTNQSDYSRFGSSNWAIW
AGTICALLIPTTLIPVFGVIGASTCDKLYGEQYWMPMDIFNHWLTTNYSAGARAGAFF
CGLSFVLSQMSYTISNCGFASGMDLAGLLPKYVDIKRGALFAACVSWACLPWNFYNSS
STFLTVMSSFGVVMTPIISVMICDNFLIRKRQYSITNAFILKGEYYFTKGVNWRAIVA
WVCGMTPGLPGIAWEVNNDYFHNTGIVNFFYGDSFFSFLISFFMYWGLCIFFPFKITV
KHDDKDYYGAFTDEEARKKGMVPYSEISEEEIRAYTLGEGYTTGHEYRPEGSDDEIPE
LVKTSSENTNEFEIVHHKNNEKQSSTASEKAA"
gene 565524..566960
/gene="Far10"
/locus_tag="R008_L11841"
mRNA 565524..566960
/gene="Far10"
/locus_tag="R008_L11841"
/product="Far10p"
CDS 565524..566960
/gene="Far10"
/locus_tag="R008_L11841"
/note="Similar to YLR238W 'Protein involved in recovery
from cell cycle arrest in response to pheromone, in a
Far1p-independent pathway; interacts with Far3p, Far7p,
Far8p, Far9p, and Far11p; potential Cdc28p substrate'"
/codon_start=1
/product="Far10p"
/protein_id="EWG84471.1"
/translation="MTGPGPEINKEEHPSSPGKKQITYNSIPKNANLIDGSTNSSKRP
IEKYDKRIADPTKSYFPHSISRTPRRKYTYILVLTSLNGTFESKHVVIPFKPDGLKLG
RPVANSNSSSSSSLRGGKRVDSHTFSQVRSDNGNFDSRVLSRNHALLSCDPLTGKVYI
RDLKSSNGTFINGQRIGSNDVEIKVGDVIDLGTDIDTKIEHRKISATVEELFVQPLLE
SPIFENEDSDDCHTITEKEEAAAITSHIYGDSNNLELEEVILGSDTEILSGIFINNCI
GTSPTLSNIIKTLAMEIPFSKCDNFKLQSMENFLINYTTHLEYTNKLLVEKNDQQLVK
LQNGLRRKLSGKYEKIIEQNRNQVKQLERDHMFFKKSFEVKKRRNNEKQKSMEREIED
LKTRLEVERYKNSQMMKKNKQKEQELSTASKKKTTEHDTRGVPGMNPKGTDKFSIKNT
LCNHFTLLTFGTISIGIIAIVFKILSPN"
gene complement(567100..568086)
/gene="Lip2"
/locus_tag="R008_L11846"
mRNA complement(567100..568086)
/gene="Lip2"
/locus_tag="R008_L11846"
/product="Lip2p"
CDS complement(567100..568086)
/gene="Lip2"
/locus_tag="R008_L11846"
/note="Similar to YLR239C Lipoyl ligase, involved in the
modification of mitochondrial enzymes by the attachment of
lipoic acid groups"
/codon_start=1
/product="Lip2p"
/protein_id="EWG84472.1"
/translation="MSRCIRQSVCTNFNVCRRQCFSTYASALKEMTHPIKPSAHTLRH
LQFTQRIPFQKGLEIQETLVRANLDIKDIQSKIERKLIQLDEEYKGTATINDNEKRIL
DKVMAMKPNPIILTFEFEPTYTGGKRIKKTMTPDQIAAYESFIPETQKDNPRPKFVQV
ERGGQVTFHGPGQIVIYIILDLKTFQSFPAKCLVSCIEQATIRTLKNTKMCDDTDKPL
NLDAMTTKDTGVWVENGKKKVASVGIHVRRSITSHGVAINVNTDLSYMNSFEMCGLKN
TLTTSIMEQRPDAVVNVQSVAISFVKEMTKLLGIKTLERMQIDDVNILKKNP"
assembly_gap 568161..568186
/estimated_length=26
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 568303..570930
/gene="Vps34"
/locus_tag="R008_L11851"
mRNA 568303..570930
/gene="Vps34"
/locus_tag="R008_L11851"
/product="Vps34p"
CDS 568303..570930
/gene="Vps34"
/locus_tag="R008_L11851"
/note="Similar to YLR240W 'Phosphatidylinositol 3-kinase
responsible for the synthesis of phosphatidylinositol
3-phosphate; forms membrane-associated signal transduction
complex with Vps15p to regulate protein sorting; activated
by the GTP-bound form of Gpa1p'"
/codon_start=1
/product="Vps34p"
/protein_id="EWG84473.1"
/translation="MSLNNITFCVSQDLDVPLKVKIKSLEGHKPLLKPSQKILNPELM
LIGSNVFPSSDLIVSLQVFDKERNRNLTLPIYTPYIPFRNSRTWDYWLTLPIRIKQLT
FSSHLRIILWEYNGSKQIPFFNLETSIFNLKDCTLKRGFESLKFRYDVIDHCEVVTDN
KDQENLNKYFQGEFTRLPWLDEITISKLRKQRENRTWPQGTFVLNLEFPMLELPVVFI
EREIMNTQMNIPTLKNNPGLSTDLREPNRNDPQIKISLGDKYHSTLKFYDPDQPNNDP
IEEKYRRLERASKNANLDKQVKPDIKKRDYLNKIINYPPGTKLTAHEKGSIWKYRYYL
MNNKKALTKLLQSTNLREESERVEVLELMDSWAEIDIDDALELLGSTFKNLSVRSYAV
NRLKKASDKELELYLLQLVEAVCFENLSTFSDKSNSEFTIVDAVSSQKLSGDSMLLST
SHANQKLLKSISSESETSGTESLPIVISPLAEFLIRRALVNPRLGSFFYWYLKSESED
KPYLDQILSSFWSRLDKKSRNILNDQVRLINVLRECCETIKRLKDTTAKKMELLVHLL
ETKVRPLVKVRPIALPLDPDVLICDVCPETSKVFKSSLSPLKITFKTTLNQPYHLMFK
VGDDLRQDQLVVQIISLMNELLKNENVDLKLTPYKILATGPQEGAIEFIPNDTLASIL
SKYHGILGYLKLHYPDENATLGVQGWVLDNFVKSCAGYCVITYILGVGDRHLDNLLVT
PDGHFFHADFGYILGQDPKPFPPLMKLPPQIIEAFGGAESSNYDKFRSYCFVAYSILR
RNAGLILNLFELMKTSNIPDIRIDPNGAILRVRERFNLNMSEEDATVHFQNLINDSVN
ALLPIVIDHLHNLAQYWRT"
gene 571243..573591
/locus_tag="R008_L11856"
mRNA 571243..573591
/locus_tag="R008_L11856"
/product="hypothetical protein"
CDS 571243..573591
/locus_tag="R008_L11856"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84474.1"
/translation="MTSYIERLKSAASYLDTVPDEHHDFRKPTAKVVTTQLTIATSLG
IFALLSFSILLKKWPRLYASRRYKDDGNLRLPSWNQSSLFGWLTVLYKIRDEQILEYA
GLDAYVFLSFFKMCIKLLSIFCFFSVCVISPVRYHFTGKIDDGNDDDDSESSLIHLVK
RIVEGSGDGDNHSAPERTNVYLWMYVLFTYFFTFIAIKMAVAETKHVVSTRQAYLGKQ
NTITDRTIRLSGIPIELRDSEALKTRIEQLKIGTVSSITICREWGPLNKLFHCRKKIL
KNLELKYSECPRELRTRQPYSENYHLLGNEQSGAVTHGENVPSSNNNDEDTILYSQIS
LGERPKMKIGYRGIFGKEVDAIEYLEQQLKFIDAEIIEARKQHYSATPTAFVTMDSVA
NAQMAAQAVLDPRVHYFITRLAPAPHDIKWDHVCLSRKDRLTKVYSTTVFIGLSSLFL
VIPVSYLATLLNLKTLSKFWPSVGQLLKDHQWAANIVTGLLPTYLFTLLNFGIPYFYE
YLTSYQGLVSYSEEEISLVSKNFFYIFVNLFLVFTLAGTASNYWAYLSDTTKIAYQLA
TSVKEFSLFYVDLIILQGIGMFPFKLLLVGSLIGFPLVKIKAKTPRQRNELYNPPIFN
FGLQLPQPILILIITLIYSVMSTKILTSGLAYFIIGFYVYKYQLIFATDHLPHSTGKV
WPLIFRRIIVGLLLFQLTMTGTLAGFEGGWVLSSCLFPLPVVTLCFLYDFEKNYLPLS
KYIALSSIREYERDNSTVNSANEEESYAYPYAVSELEGPMLD"
gene complement(573688..574653)
/gene="Arv1"
/locus_tag="R008_L11861"
mRNA complement(573688..574653)
/gene="Arv1"
/locus_tag="R008_L11861"
/product="Arv1p"
CDS complement(573688..574653)
/gene="Arv1"
/locus_tag="R008_L11861"
/note="Similar to YLR242C 'Protein functioning in
transport of glycosylphosphatidylinositol intermediates
into ER lumen; required for normal intracellular sterol
distribution; human ARV1 required for normal cholesterol
and bile acid homeostasis; similar to Nup120p'"
/codon_start=1
/product="Arv1p"
/protein_id="EWG84475.1"
/translation="MICITCMRPVDSLYTVYSNDHIQLTDCPYCQETVDKYVEIDNVL
LFIDLLLLKAGAYRHLVFNALELHLSKYPKRKALNDCQCLRDYTQALLFNVKNWFCKY
DRLNRLWLLLLSFEIYLTWVTEESKYIYYLNRNNNDGKLIMLSKKLPESFKWDSAIMR
NTITSKVFTWSPPIQYLYFASYCILDVSLFHTFTQYFILKKLHWKHYSVSSKDVISYT
ILLSYGAKIFPILMLIWPYDTLISMSIIKWVANLYIIESLKIVTNLSYWNIIKIFISV
SLLRYFMVKPILIVFVAKFNFSVIKNLIHQEFILLLQKSGTYLLL"
gene 574973..575791
/locus_tag="R008_L11866"
mRNA 574973..575791
/locus_tag="R008_L11866"
/product="hypothetical protein"
CDS 574973..575791
/locus_tag="R008_L11866"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84476.1"
/translation="MSRVGVMVLGPAGAGKSTFCNSIISHMQTVGRRAHIVNLDPAAE
ATKYEFTIDIRDLISLDDVMEEMDLGPNGALIYCFEYLLKNLDWLDEEIGDFNDEYLI
FDCPGQIELYTHIPVLPNIVRHLTQQLNFNLCATYLLEAPFVIDSSKFFSGALSAMSA
MILLELPHINVLSKLDLIKGDINKKKLKRFLNPDAMLLMETEGMNQASNPKFLRLNQC
IANLVDDFGMVQFLPLESNNPDSIETILSYVDDITQWAEGQEQKEPNDQIDVEE"
gene complement(575939..577102)
/gene="Map1"
/locus_tag="R008_L11871"
mRNA complement(575939..577102)
/gene="Map1"
/locus_tag="R008_L11871"
/product="Map1p"
CDS complement(575939..577102)
/gene="Map1"
/locus_tag="R008_L11871"
/note="Similar to YLR244C 'Methionine aminopeptidase,
catalyzes the cotranslational removal of N-terminal
methionine from nascent polypeptides; function is
partially redundant with that of Map2p'"
/codon_start=1
/product="Map1p"
/protein_id="EWG84477.1"
/translation="MSTATTTVTTSDQASHPIKIYCSGLQCGRETSSQMKCPVCLKQG
IVSIFCDTSCYENNYKAHKALHNAKDGLEGAYDPFPKFKYSGKVKASYPLTPRRYVPE
DIPKPDWAANGLPVSEQRNDRLNNIPIYKKDQIKKIRKACMLGREVLDIAAAHVRPGI
TTDELDEIVHNETIKRGAYPSPLNYYNFPKSLCTSVNEVICHGVPDKTVLKEGDIVNL
DVSLYYQGYHADLNETYYVGENISKEALNTTETSRECLKLAIKMCKPGTTFQELGDHI
EKHATENKCSVVRTYCGHGVGEFFHCSPNIPHYAKNRTPGVMKPGMVFTIEPMINEGT
WKDMTWPDDWTSTTQDGKLSAQFEHTLLVTEHGVEILTARNKKSPGGPRQRIK"
assembly_gap 577204..577236
/estimated_length=33
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(577277..577699)
/gene="Cdd1"
/locus_tag="R008_L11876"
mRNA complement(577277..577699)
/gene="Cdd1"
/locus_tag="R008_L11876"
/product="Cdd1p"
CDS complement(577277..577699)
/gene="Cdd1"
/locus_tag="R008_L11876"
/note="Similar to YLR245C 'Cytidine deaminase; catalyzes
the modification of cytidine to uridine in vitro but
native RNA substrates have not been identified, localizes
to both the nucleus and cytoplasm'"
/codon_start=1
/product="Cdd1p"
/protein_id="EWG84478.1"
/translation="MKVGGIEDRQLEALKRAALKACELSYSPYSHFRVGCSILTNNDV
IFTGANVENASYSNCICAERSAMIQVLMAGHRSGWKCMVICGDSEDQCVSPCGVCRQF
INEFVVKDFPIVMLNSTGSRSKVMTMGELLPWLLVHLI"
gene 577887..578966
/gene="Erf2"
/locus_tag="R008_L11881"
mRNA 577887..578966
/gene="Erf2"
/locus_tag="R008_L11881"
/product="Erf2p"
CDS 577887..578966
/gene="Erf2"
/locus_tag="R008_L11881"
/note="Similar to YLR246W 'Subunit of a
palmitoyltransferase, composed of Erf2p and Shr5p, that
adds a palmitoyl lipid moiety to heterolipidated
substrates such as Ras1p and Ras2p through a thioester
linkage; mutants partially mislocalize Ras2p to the
vacuole'"
/codon_start=1
/product="Erf2p"
/protein_id="EWG84479.1"
/translation="MALVSRRSTRSESTSITKEEHTGEGSLTKLFFRWLVTLEGDQDI
NDGKGYISIPNVSNYIFFLGGRFRTVKGAKPLWLGVLFAIICPMVLFSIFEAHKLWHT
QNGYKVLVIFFYYFWVITLASFIRTATSDPGVLPRNIHLSQLRNNYQIPQEYYNLITL
PTHSSISKDITIKYCPSCRIWRPPRSSHCSTCNVCVMVHDHHCIWVNNCIGKRNYRFF
LIFLLGAILSSVILLTNCAIHIARESGGPRDCPVAILLLCYAGLTLWYPAILFTYHIF
MAGNQQTTREFLKGIGSKKNPVFHRVVKEENIYNKGSFLKNMGHLMLEPRGPSFVSAR
KPHEAGDWRFMDLSPAHSFEKIQKI"
gene 579151..579265
/locus_tag="R008_L11886"
tRNA 579151..579265
/locus_tag="R008_L11886"
/product="tRNA-Leu"
/note="TL(CAA)L"
gene complement(579452..584122)
/gene="Irc20"
/locus_tag="R008_L11891"
mRNA complement(579452..584122)
/gene="Irc20"
/locus_tag="R008_L11891"
/product="Irc20p"
CDS complement(579452..584122)
/gene="Irc20"
/locus_tag="R008_L11891"
/note="Similar to YLR247C 'Putative helicase; localizes to
the mitochondrion and the nucleus; YLR247C is not an
essential gene; null mutant displays increased levels of
spontaneous Rad52p foci'"
/codon_start=1
/product="Irc20p"
/protein_id="EWG84480.1"
/translation="MSEVGALLAREYNVTAERCDFFLENGSFDSVIAALPALNQEQET
VTQKVSKNGKELINVASVNIEIPEQISLSNNGRQLFKFIVDIEILPCENDNEATLVVS
SDSVSLFQIGFKMENNSKIAVDKQLPLILDICAHKNQERALRNQLEDRKSLERKPSRK
RRKKNSNVNDPEILLKSRIHELSLSYKDFECSPVVFRYNDSSLTWVLSFNLKLKFLNN
KFNRFSVEANQILDLTFSNRDENEFERYHKHSHIHSNFIQKQFISQILEYSKDRLSKI
KPFLPQSIPDLKVNLLPFQRESVEWMLIKEGHGNSLSDTPTVIDEVGLRDFMNEYYAY
GYELIARSPDEVGPSLLWNKLTGYILTTEDAAHLYNQYRKERLSGDYPVCAKGVLAEE
MGLGKTIEILSLILLNRRKLKDSEATFIDDENRRITKAKTTLIICPNAILKQWLEDIE
LHANSLKWYTYRGYNEIMKDCKTVDEAVQQLCQYDIIVTSYNIIATEVHHAEFNRSIR
SRRLKSPKYDYSSPLALMQFYRIILDEVQMLRSSSTYSAKCTSLLHRIHTWGVSGTPI
QNIYNFRMIMSYLKLHPFCDEVDFIRTLQEEIKLRNEAKDYTSNDFVCQLKGVRFSIK
DCMNIFYRYDLCIRHSKANVASQIHIPRQHNFIIPLEFAPIEWDNYLNLWNNFLELSG
YNSDGSGSPRVSNAFLNEWLSRLRYICCHALFPEILSTRQKRLHGHLSRISNIDDILI
SMRMDAFDSLIGYYRERFHLSIKQAQYELEISNTPAKALESFIKIRDDLMIHIRQKFN
VEDPFDKSLNLSEDGDEHMDERFGEKETSSSDESDREINGAKNHDNHNNDGMLSNHLK
KKGLRAMMNLLHDCYFFLGSVYYNLGTRKLEEADDKHRKEKTEEVVYSDVFPKNELEE
IEENRLLEQENYANAEILRKSILSSEARKVDMTIKMARTKFAPMTSNIPLRLINIEFD
HKNDYSSNLAVSRCFKSLSKLIEGLNEQTKNFNELLDELLITIYEPVHRTEDDDSTNK
IIGNEEYSTSIDSQDKIFSLLGCLEIILQNRDNILTSESEVKIPKHLVPEGSIISKYQ
KQLLNSLRLISGTPLRTVFDELKNSRIVRRISSSNESESTIQNFEDYLLQYEVESKSL
FKYNKQVRESLKILGSIYNAKTEYYSQLQRISDSLVSLHSLSAPQLSHLIRTINKSLG
GTLDAKINNIESRLIYLRNLSRLKDTLNDNQILSCSICLGEVEIGAIIKCGHYFCKSC
ILTWLRAHSKCPICKGFCSISEVYNFKFKNSTEKREKEIQEPQREGADSSQDNSNENS
IISNMSEVEKLFGNKYEQFHQINEVHQIHIKESFGAKIDFVIKLISYLRLKSEQENAD
PPQVILYSQKTEYLKVIGKVLKLYHIEHLACLSNTANVGETINNFKRQPSVTCLLLNV
KTLGAGLNLINAKHIFLLDPILNNSDELQAMGRNNRIGQDEETFVWNFMIRNTVEENI
LRYKCILEERKRKEKSKKGDKYDEAQDETDNEESDDAKFEISVVDQEVSNEHLWNCFF
HGSD"
gene 585017..586849
/gene="Rck2"
/locus_tag="R008_L11896"
mRNA 585017..586849
/gene="Rck2"
/locus_tag="R008_L11896"
/product="Rck2p"
CDS 585017..586849
/gene="Rck2"
/locus_tag="R008_L11896"
/note="Similar to YLR248W 'Protein kinase involved in the
response to oxidative and osmotic stress; identified as
suppressor of S. pombe cell cycle checkpoint mutations;
similar to CaM (calmodulin) kinases'"
/codon_start=1
/product="Rck2p"
/protein_id="EWG84481.1"
/translation="MLKIKALFSKKKPDQADLSQESKKPFKGKTRSSGTNNKDVSQIT
SSPKKSFQDKNIVQYPSVVADDHHMKSLTDELVTTIDSDSSPSDNITTENVETVTSVP
AIDVHESSEGHLSSDPLISDESLSEQSEIISDIQDDSTDDDNMEDEIPEKSFLEQKEL
IGYKLINKIGEGAFSKVFRAIPAKNSSNEFLTKNYKAVAIKVIKKADLSSINGDHRKK
DKGKDSTKTSSRDQVLKEVALHKTVSAGCSQIVAFIDFQETDSYYYIIQELLTGGEIF
GEIVRLTYFSEDLSRHVIKQLALAVKHMHSLGVVHRDIKPENLLFEPIEFTRSIKPKL
RKSDDPQTKADEGIFTPGVGGGGIGIVKLADFGLSKQIFSKNTKTPCGTVGYTAPEVV
KDEHYSMKVDMWGIGCVLYTMLCGFPPFYDEKIDTLTEKISRGEYTFLKPWWDEISAG
AKNAVSKLLELEPSKRYDIDQFLDDPWLNTFDCLPKEGESSQKKAGTSERRHPHKKQF
QLFQRDSSLLFSPAAVAMRDAFDIGNAVKRTEEDRMGTRGGLGSLAEDEELEDSYSGA
QGDEQLEQNMFQLTLDTSTILQRRKKVQENDVGPTIPISATIRE"
gene 587543..590677
/gene="Yef3"
/locus_tag="R008_L11906"
mRNA 587543..590677
/gene="Yef3"
/locus_tag="R008_L11906"
/product="Yef3p"
CDS 587543..590677
/gene="Yef3"
/locus_tag="R008_L11906"
/note="Similar to YLR249W 'Gamma subunit of translational
elongation factor eEF1B, stimulates the binding of
aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A
(Tef1p/Tef2p) from the ribosomal complex; contains two ABC
cassettes; binds and hydrolyzes ATP'"
/codon_start=1
/product="Yef3p"
/protein_id="EWG84482.1"
/translation="MSDSQQSIKVLEELFQKLSVATADNRHEIASEVASFLNGNIIEH
DVPEHFFGELAKGIKDKKTAANAMQAVAHIANQSNLSPSVEPYIVQLVPAICTNAGNK
DKEIQSVASETLISIVNSVNPVAIKALLPHLTNAIVETNKWQEKIAILAAFSAMVDAA
KDQVALRMPELIPVLSETMWDTKKEVKAAATAAMTKATETVDNKDIERFIPSLIQCIA
DPTEVPETVHLLGATTFVAEVTPATLSIMVPLLSRGLNERETGIKRKSAVIIDNMCKL
VEDPQVIAPFLGKLLPGLKSNFATIADPEAREVTLRALKTLRRVGNVGEDDAIPEVSH
AGDVSTTLQVVNELLKDETVAPRFKIVVEYIAAIGADLIDERIIDQQAWFTHITPYMT
IFLHEKKAKDILDEFRKRAVDNIPVGPNFDDEEDEGEDLCNCEFSLAYGAKILLNKTQ
LRLKRARRYGICGPNGCGKSTLMRAIANGQVDGFPTQEECRTVYVEHDIDGTHSDTSV
LDFVFESGVGTKEAIKDKLIEFGFTDEMIAMPISALSGGWKMKLALARAVLRNADILL
LDEPTNHLDTVNVAWLVNYLNTCGITSITISHDSVFLDNVCEYIINYEGLKLRKYKGN
FTEFVKKCPAAKAYEELSNTDLEFKFPEPGYLEGVKTKQKAIVKVTNMEFQYPGTSKP
QITDINFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTSGEVYTHENCRIAYIKQHA
FAHIESHLDKTPSEYIQWRFQTGEDRETMDRANRQINENDAEAMNKIFKIEGTPRRIA
GIHSRRKFKNTYEYECSFLLGENIGMKSERWVPMMSVDNAWIPRGELVESHSKMVAEV
DMKEALASGQFRPLTRKEIEEHCSMLGLDPEIVSHSRIRGLSGGQKVKLVLAAGTWQR
PHLIVLDEPTNYLDRDSLGALSKALKEFEGGVIIITHSAEFTKNLTEEVWAVKDGRMT
PSGHNWVSGQGAGPRIEKKEDEEDKFDAMGNKIAGGKKKKKLSSAELRKKKKERMKKK
KELGDAYVSSDEEF"
gene 591082..591786
/gene="Ssp120"
/locus_tag="R008_L11911"
mRNA 591082..591786
/gene="Ssp120"
/locus_tag="R008_L11911"
/product="Ssp120p"
CDS 591082..591786
/gene="Ssp120"
/locus_tag="R008_L11911"
/note="Similar to YLR250W 'Protein of unknown function;
green fluorescent protein (GFP)-fusion protein localizes
to the cytoplasm in a punctate pattern'"
/codon_start=1
/product="Ssp120p"
/protein_id="EWG84483.1"
/translation="MRFLRGFVFSLAFTLYKVTATAEIGSEINVENEAPPDGLSWEEW
HMDHEHQLKDYTPETFFALHDIKKKGFLDENDILSLYGLNREEIVGAGDGMGQHDESE
KIDNEMAKRVVSLIMRLLDVDDNTKITKEEYLQFAKRGNKFPDLGVGVGHHSDFELEY
EIHHWNKFHKDKDPDVKVVHKEDIEHELLHHEHEIEHEEEIQRGASRATVITDDELES
RIELKNIPEKFKNGIF"
gene 592227..592820
/gene="Sym1"
/locus_tag="R008_L11916"
mRNA 592227..592820
/gene="Sym1"
/locus_tag="R008_L11916"
/product="Sym1p"
CDS 592227..592820
/gene="Sym1"
/locus_tag="R008_L11916"
/note="Similar to YLR251W 'Protein required for ethanol
metabolism; induced by heat shock and localized to the
inner mitochondrial membrane; homologous to mammalian
peroxisomal membrane protein Mpv17'"
/codon_start=1
/product="Sym1p"
/protein_id="EWG84484.1"
/translation="MKLLHFYEASLKRRPKTTNAIMTGALFGIGDVSAQLLFPTSKVN
KGYDYKRTARAVIYGSLIFSFIGDKWYKILNNKIYMRNRPQYHWSNMVLRVAVDQLAF
APLGLPFYFTCMSIMEGRSFDVAKLKIKEQWWPTLLTNWAVWPLFQAINFSVVPLQHR
LLAVNVVAIFWNTYLSYKNSKVMEKDKVPVHYPPVVE"
gene 593390..595099
/locus_tag="R008_L11926"
mRNA 593390..595099
/locus_tag="R008_L11926"
/product="hypothetical protein"
CDS 593390..595099
/locus_tag="R008_L11926"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84485.1"
/translation="MMTKAFFNKLPFEVFRRYVRTGKSIPQRSPRTRKSLLVGGTIAS
AVVLYNFNDTFHDSVKHTALTTKRIAVVTQATTRCFYHYKRALNKSYENKKEREVALN
KCHKMCALITLHALRSNGGIYIKLGQHIGAMTYLLPKEWTDTMIPLQDHCPESTYEEI
DELFKEDLGTSIEDMFLEFNKTPIGVASLAQVHVAKLKNSDGKGSSVAVKCQHPSLKE
FIPLDVMLTRTVFELLDVFFPDYPLTWLGDELQSSIYVELNFTKEAENAEKTRHYFSK
FKKQTALKIPKVIESHKRILIMEYVGGKRLDDLEYIDSHGISRSEVSSCLSHIFNNMI
FTPNVGIHCDPHGGNLAIRSVKPAKDNGYHNFEIVLFDHGLYRYPSTRTRRLYAKFWL
SLLFDKDQTKMKKYAKGFANITDEQFPLLAAAITGRSVDAALNYDISTSRTQEEMDVM
ANGILEGTLLSDLMSILSRIPRVVLLILKTNDLTRHLDECLQNPLGPERTFLIMTQYC
AKTVYDEKVERINSEYARWSIKWMWENLTNWIVYERRINQLYFYDFVLWWKKFIPKTW
LSS"
gene complement(595168..595737)
/gene="Ndl1"
/locus_tag="R008_L11931"
mRNA complement(595168..595737)
/gene="Ndl1"
/locus_tag="R008_L11931"
/product="Ndl1p"
CDS complement(595168..595737)
/gene="Ndl1"
/locus_tag="R008_L11931"
/note="Similar to YLR254C 'Homolog of nuclear distribution
factor NudE, NUDEL; interacts with Pac1p and regulates
dynein targeting to microtubule plus ends'"
/codon_start=1
/product="Ndl1p"
/protein_id="EWG84486.1"
/translation="MVPNLDLETAIQIISSLETQLSELEGATKEYENDLEQVISKLKS
DLLESQQQNKCNKKQITDLEIQVDELENENIQLRNKIETLQLESDRRLERNVLLEHEL
LDTKEALQKLRVSKEEATSGETRRNTRSLPSQNKKMKLFKDTIKVSTTSSTLYLQNMA
KTNNAARSHCNIPNTQITQSTVIATTSSV"
gene 597207..601715
/gene="Hap1"
/locus_tag="R008_L11941"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(601801..601873)
/locus_tag="R008_L11946"
tRNA complement(601801..601873)
/locus_tag="R008_L11946"
/product="tRNA-Ala"
/note="tRNA-Ala (Ala1);
TA(AGC)L"
gene 603695..604660
/locus_tag="R008_L11956"
mRNA 603695..604660
/locus_tag="R008_L11956"
/product="hypothetical protein"
CDS 603695..604660
/locus_tag="R008_L11956"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84487.1"
/translation="MVDARGSTPCLIGDSIRNVNDGNSLDFQYTNQFNEESEASRLLT
PQTSSNHALSKMQKDDDIRDRSYTSVAELNREGALLTDEVDLENVDASKVRSNRDDLE
AEEKRKKLLLLKKKQRNKSINSESFSSPSLRASKSNSLITSTDPVEDHISKYSSSGTP
ENITGEADDEDEDIIRNSYGQMIKNNSNRPHLAKGESYQSAEQEIDHTAPEKSENRQE
RSGRSFDRQKSSAEFLRSLSRSISRGPTKNKTVSPSKGEDSRMYSTSNYSISLVDLEN
GPKIIPETLEEEQEDAEKEGVLMEDEGNEEYTKDLEEAANKAQPQ"
gene 605585..607702
/gene="Gsy2"
/locus_tag="R008_L11966"
mRNA 605585..607702
/gene="Gsy2"
/locus_tag="R008_L11966"
/product="Gsy2p"
CDS 605585..607702
/gene="Gsy2"
/locus_tag="R008_L11966"
/note="Similar to YLR258W 'Glycogen synthase, similar to
Gsy1p; expression induced by glucose limitation, nitrogen
starvation, heat shock, and stationary phase; activity
regulated by cAMP-dependent, Snf1p and Pho85p kinases as
well as by the Gac1p-Glc7p phosphatase'"
/codon_start=1
/product="Gsy2p"
/protein_id="EWG84488.1"
/translation="MSRDLQNHLLFETATEVANRVGGIYSVLKSKAPITVAQYKDHYH
LIGPLNKATYQNEVDILDWKKPEAFSDEMRPVQHALQTMESRGVHFVYGRWLIEGAPK
VILFDLDSVRGYSNEWKGDLWSLVGIPSPENDFETNDAILLGYTVAWFLGEVAHLDSQ
HAIVAHFHEWLAGVALPLCRKRRIDVVTIFTTHATLLGRYLCASGSFDFYNCLESVDV
DHEAGRFGIYHRYCIERAAAHSADVFTTVSQITAFEAEHLLKRKPDGILPNGLNVIKF
QAFHEFQNLHALKKEKINDFVRGHFHGCFDFDLDNTLYFFIAGRYEYKNKGADMFIEA
LARLNYRLKVSGSKKTVVAFIVMPAKNNSFTVEALKGQAEVRALENTVHEVTTSIGKR
IFDHAIRYPHNGLTTELPTDLGELLKSSDKVMLKRRILALRRPEGQLPPIVTHNMVDD
ASDLILNKIRQVQLFNSPSDRVKMIFHPEFLNANNPILGLDYDEFVRGCHLGVFPSYY
EPWGYTPAECTVMGVPSITTNVSGFGSYMEDLIETNQAKDYGIYIVDRRFKAPDESVE
QLVDYMEEFVKKTRRQRINQRNRTERLSDLLDWKRMGLEYVKARQLALRRGYPDQFRE
LVGEELNDSNMDALAGGKKLKVARPLSVPGSPRDLRSNSTVYMTPGDLGTLQEVNNAD
DYFSLGVNPAADDDDDGPYADDS"
gene complement(608153..609871)
/gene="Hsp60"
/locus_tag="R008_L11971"
mRNA complement(608153..609871)
/gene="Hsp60"
/locus_tag="R008_L11971"
/product="Hsp60p"
CDS complement(608153..609871)
/gene="Hsp60"
/locus_tag="R008_L11971"
/note="Similar to YLR259C 'Tetradecameric mitochondrial
chaperonin required for ATP-dependent folding of precursor
polypeptides and complex assembly; prevents aggregation
and mediates protein refolding after heat shock; role in
mtDNA transmission; phosphorylated'"
/codon_start=1
/product="Hsp60p"
/protein_id="EWG84489.1"
/translation="MLRSSVVRSRATLRPLLRRAYSSHKELKFGVEGRASLLKGVETL
AEAVAATLGPKGRNVLIEQPFGPPKITKDGVTVAKSIVLKDKFENMGAKLLQEVASKT
NEAAGDGTTSATVLGRAIFTESVKNVAAGCNPMDLRRGSQVAVEKVIEFLSANKKEIT
TSEEIAQVATISANGDSHVGKLLASAMEKVGKEGVITIREGRTLEDELEVTEGMRFDR
GFISPYFITDPKSSKVEFEKPLLLLSEKKISSIQDILPALEISNQSRRPLLIIAEDVD
GEALAACILNKLRGQVKVCAVKAPGFGDNRKNTIGDIAVLTGGTVFTEELDLKPEQCT
IENLGSCDSITVTKEDTVILNGSGPKEAIQERIEQIKGSIDITTTNSYEKEKLQERLA
KLSGGVAVIRVGGASEVEVGEKKDRYDDALNATRAAVEEGILPGGGTALVKASRVLDE
VVVDNFDQKLGVDIIRKAITRPAKQIIENAGEEGSVIIGKLIDEYGDDFAKGYDASKS
EYTDMLATGIIDPFKVVRSGLVDASGVASLLATTEVAIVDAPEPPAAAGAGGMPGGMP
GMPGMM"
gene 610714..612750
/gene="Lcb5"
/locus_tag="R008_L11976"
mRNA 610714..612750
/gene="Lcb5"
/locus_tag="R008_L11976"
/product="Lcb5p"
CDS 610714..612750
/gene="Lcb5"
/locus_tag="R008_L11976"
/note="Similar to YLR260W Minor sphingoid long-chain base
kinase, paralog of Lcb4p responsible for few percent of
the total activity, possibly involved in synthesis of
long-chain base phosphates, which function as signaling
molecules"
/codon_start=1
/product="Lcb5p"
/protein_id="EWG84490.1"
/translation="MTLKPSKRRKGRSRHSRKKQITSAILTEEGIMIKAKPSSPYTYA
NRMADKRSRSSIDNISRTSFQSNSDNNSIFETASLISCVTCLSDTDTIDRSETSTTDT
SKDDLSANPKLHYPSVNGQLPANTVIPYGRILDARYIEKEPLHYYDANSSPSSPLSSS
MSNISEKCDLDELESSQKKERKGNSLSRGSNSSSSLLTSRSPFTKLVEVIFARPRRHD
VVPKRVSLYIDYKPHSSSHLKEEDDLVEEILKRSYKNTRRNKSIFVIINPFGGKGKAK
KLFMTKAKPLLLASRCSIEVVYTKYPGHAIEIAREMDIDKYDTIACASGDGIPHEVIN
GLYQRPDHVKAFNNIAITEIPCGSGNAMSVSCHWTNNPSYSTLCLIKSIETRIDLMCC
SQPSYAREHPKLSFLSQTYGLIAETDINTEFIRWMGPARFELGVAFNIIQKKKYPCEI
YVKYAAKSKNELKNHYLEHKNKGSLEFQHITMNKDNEDCDNYNYENEYETENEDEDED
ADADDEDSHLISRDLADSSADQIKEEDFKIKYPLDEGIPSDWERLDPNISNNLGIFYT
GKMPYVAADTKFFPAALPSDGTMDMVITDARTSLTRMAPILLGLDKGSHVLQPEVLHS
KILAYKIIPKLGNGLFSVDGEKFPLEPLQVEIMPRLCKTLLRNGRYVDTDFDSM"
gene complement(613089..613736)
/gene="Ypt6"
/locus_tag="R008_L11981"
mRNA complement(613089..613736)
/gene="Ypt6"
/locus_tag="R008_L11981"
/product="Ypt6p"
CDS complement(613089..613736)
/gene="Ypt6"
/locus_tag="R008_L11981"
/note="Similar to YLR262C 'Rab family GTPase, Ras-like GTP
binding protein involved in the secretory pathway,
required for fusion of endosome-derived vesicles with the
late Golgi, maturation of the vacuolar carboxypeptidase Y;
has similarity to the human GTPase, Rab6'"
/codon_start=1
/product="Ypt6p"
/protein_id="EWG84491.1"
/translation="MSRSGKSLTKYKIVFLGEQGVGKTSLITRFMYDTFDDHYQATIG
IDFLSKTMYLDDKTIRLQLWDTAGQERFRSLIPSYIRDSRVAIIVYDITKRKSFEYID
KWIEDVKNERGDENVILCIVGNKSDLSDERQISTEEGEKKAKLLGAKIFMETSTKAGY
NVKALFKKIAKSLPEFQNSESTPLDSENANSANQNKPGVIDISTAEEQEQSACQC"
gene complement(614313..614507)
/gene="Tma7"
/locus_tag="R008_L11991"
mRNA complement(614313..614507)
/gene="Tma7"
/locus_tag="R008_L11991"
/product="Tma7p"
CDS complement(614313..614507)
/gene="Tma7"
/locus_tag="R008_L11991"
/note="Similar to YLR262C-A no_gene_description"
/codon_start=1
/product="Tma7p"
/protein_id="EWG84492.1"
/translation="MSSRQGGKMKPLKQKKKQQQDLDPEDIAFKEKQKADAAAKKALM
ANMKSGKPLVGGGIKKSGKK"
gene 615180..617663
/gene="Red1"
/locus_tag="R008_L11996"
mRNA 615180..617663
/gene="Red1"
/locus_tag="R008_L11996"
/product="Red1p"
CDS 615180..617663
/gene="Red1"
/locus_tag="R008_L11996"
/note="Similar to YLR263W 'Protein component of the
synaptonemal complex axial elements, involved in
chromosome segregation during the first meiotic division;
critical for coupling checkpoint signaling to SC
formation; interacts with Hop1p, Mec3p and Ddc1p'"
/codon_start=1
/product="Red1p"
/protein_id="EWG84493.1"
/translation="MEGLKKKIFGVCLKNDLAQTRNETKGIHYGLMTLETSKQLEEFL
HLLVIKREVIQNFELLFHIINVAVKITDSNLPSDDIWHFILKLRFSSEINIDEDSKVL
NYLLETGIAMENPVSWKCLAVISSILSSVPQSKKIITNLIETEHAKKIGQLFDNIQDL
QQGNFLVEILSNCFKKSASNSKKVEKIPQLWQSRSKNKFFFENEFYPFSSKNGSLQTC
QFLCNNFMSTLSFTGILRQVSYSGSETLKNLRIFKKKDDENSYFIQCIYNKIYLWLDE
KAPLEFERKKIRITKNLKNKIQIKLRQPFHECVRTTADKTALLFNKTRGFQLEFEDEK
LGETFFHNVNNVPKISEVQNFLVLDYIEEEPENEGEEEEQTRRADEQKEDEEEESLDE
LSTPMVYPIKSSISHNHNEKVQLVTPDRSVSIRSDEWDLKSNTEDEEGNVLTDLKISS
TKETRRQTDYVHIDSEDQSPVVSAQMRKMRRESTKTLEILRQEFKDKDVQNKEDQSEQ
IQNPFVNTSSLVVGKSCLVNPKEKPNIDQTVVGITELKSNSSIKKRDINILDTIFGQP
PSKKQKQFHKKEKKKQQKKLTNFKPIIDVPSQDKRNLRSNAPTKPKSIKVSKLRTDKK
VTGEKSSPETTAEKVDDQTVRSNDEQAVSRATKEKRFPDVNEGKEITNDDAKVSLESK
KNNETFVDSSVVEKHTPPDKDCNNCNITDILESTTVIDLHSPHGLSAPGQNTFTNKLQ
EQIYSSINHFSNELVRKISIINQELNKKILKELSEKYQKLFAELQDNFQNDTNEMLKF
MGEIKDMMNLPEDQLVHAIRTRKFDNNKR"
gene 617970..618173
/gene="Rps28b"
/locus_tag="R008_L12001"
mRNA 617970..618173
/gene="Rps28b"
/locus_tag="R008_L12001"
/product="Rps28bp"
CDS 617970..618173
/gene="Rps28b"
/locus_tag="R008_L12001"
/note="Similar to YLR264W 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps28Ap and
has similarity to rat S28 ribosomal protein'"
/codon_start=1
/product="Rps28bp"
/protein_id="EWG84494.1"
/translation="MDSKTPVTLAKVIKVLGRTGSRGGVTQVRVEFLEDTSRTIVRNV
KGPVRENDILVLMESEREARRLR"
gene complement(618666..618781)
/locus_tag="R008_L12006"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(619264..620292)
/gene="Nej1"
/locus_tag="R008_L12011"
mRNA complement(619264..620292)
/gene="Nej1"
/locus_tag="R008_L12011"
/product="Nej1p"
CDS complement(619264..620292)
/gene="Nej1"
/locus_tag="R008_L12011"
/note="Similar to YLR265C 'Protein involved in regulation
of nonhomologous end joining; interacts with DNA ligase IV
components Dnl4p and Lif1p; repressed by MAT
heterozygosity; regulates cellular distribution of Lif1p'"
/codon_start=1
/product="Nej1p"
/protein_id="EWG84495.1"
/translation="MDSELKGQQLSDAEWCIKKIIGEGNCLLLFLPMSSPTTIVMIVL
VSLERLVPYVFKLSQTQLSQQCQSQGFTDSISLNLIKLKLMDILQAPQEINQIGLVDS
NLVFSFDVSADITVSINSVPSHVTKDMFYMILQSLCMLLLKLVNLSTQYHYVQRDILN
QKQKCLDFLLISLRDLDGGSKVISQWAPENSKNYESLQQCTDDDIIKKLLHKGKFQHQ
EFLADSLKTLLSFRNKFQDVSRFEESGELNEKERVRFPAVNHFYNDDFELQVDPTNEA
RPNSCGKIKPKTDFKPKSRESSTSSQLRLENFSESEATPEKTKSSSSLVEEYPQKKRK
FGKVRIKN"
gene complement(620456..622561)
/gene="Pdr8"
/locus_tag="R008_L12016"
mRNA complement(620456..622561)
/gene="Pdr8"
/locus_tag="R008_L12016"
/product="Pdr8p"
CDS complement(620456..622561)
/gene="Pdr8"
/locus_tag="R008_L12016"
/note="Similar to YLR266C 'Transcription factor; targets
include ATP-binding cassette (ABC) transporters, major
facilitator superfamily transporters, and other genes
involved in the pleiotropic drug resistance (PDR)
phenomenon'"
/codon_start=1
/product="Pdr8p"
/protein_id="EWG84496.1"
/translation="MDGSHFPMTSTTGEPVLSGKKGKRRKVIKSCAFCRKRKLKCSQA
RPMCQQCVIRKLPQCVYTEEFNYPLSNTELFEQVPNVALVQKIENLQTLLKENDNNNA
KPVYCRSSENPLRSLRTSVLGDNGSRYVFGPTSWKTLSLFEQNKFQTEFQNLWKVLKP
LPECTKSQLNENDVVADLPSFPQMESCIKSFFAGPLFDILHIFNQDDILSLLDRLFIR
DTTDKNLVILLDLQGNAKDKYNLGIVLQILCLGYYNQDIPSSVSRFLHSLSAASLSSS
SSNFVEKLQFFLLSYISVMINCTDGVWDATQGVDLINELCQGCISLGLNDIDKWYLNE
SEETKQNLRCIWFWALFLDVSTSYDIGNPPSISDDLFDLSIFTAQNFQSPSIDFRRVK
LMHDFLDVSRFTTREIHKREMNEKLTTFSLRLIEFIQSNFSPIEHYTNSVYYSDIDPF
DILILSRSLSIVASIYNIEMIIAQQSRIIDKNRMVQFLLISISVCVNTMVFHFKEPIN
DQENVLTEGLKLSIILINPLLIRIVSQVYSLAFNRLIFREKGFLFLIDLDTGKKIQFI
KYEEENFDELLTGFDVRTDKFLSFSGTIIRFYEIVDSIFAVNERNKRLLKAVSKFYQL
TSTLAFERVSRVLFDKASQARIETEKIWLKKGINMEHFSDLMIEDFINDVWKTFKEIS
KDLWSIDKKKFYKQYHFDL"
gene 623049..624761
/gene="Bop2"
/locus_tag="R008_L12031"
mRNA 623049..624761
/gene="Bop2"
/locus_tag="R008_L12031"
/product="Bop2p"
CDS 623049..624761
/gene="Bop2"
/locus_tag="R008_L12031"
/note="Similar to YLR267W Protein of unknown function"
/codon_start=1
/product="Bop2p"
/protein_id="EWG84497.1"
/translation="MVAALTYLPTELIQRIFEFTVVETDSQYWLYNLVALIDFSVSSR
GGGSITEEFLTNYVRKNLMVLDLTCEATQDSILRAEYGFLKRLLPYIDMDAQYIRVVD
LETNVDKAQNLKAEKLIVIFDEFSDLKLIETFFPLANSNSNITEFVFCVRNIKSSFYS
PLEKLHIANIVADIDINTLYLDFVDSNIYSDQNLFGIFDPDIFQLINKNYRNFFSKTN
EKGKKRPPICKKICFPFVETLNLDYMALDSFFNSILHKLTTKIKTFERNNEFDVDKNL
NLNSTTTVAALIIKSILQQFFNNFHISFPNLVTLNFIKMSTYPNNNEITQCCNFIDLS
SYVLNKCLSENISINFLFQLHSLKNWSMPKIKEFTGHKFKYDETTFSGSPERYIKSLR
GNIKILQEMAINETNDGTCYFRVKLIPEGVEKTQIIDWIPFTSSFSDDTFKQRHHLKR
PMICLKNNSLRSLTVKIIRIEKCSSIRIQGFYLPNLQELFINNTLCDTTQHQKQASND
MSCIEFTSWNELPQCKKLGFAQLEDDSNYVLNISNLQDHLPNLDLRESFPTFFDIRQK
FVVV"
gene 625037..625681
/gene="Sec22"
/locus_tag="R008_L12036"
mRNA 625037..625681
/gene="Sec22"
/locus_tag="R008_L12036"
/product="Sec22p"
CDS 625037..625681
/gene="Sec22"
/locus_tag="R008_L12036"
/note="Similar to YLR268W 'R-SNARE protein; assembles into
SNARE complex with Bet1p, Bos1p and Sed5p; cycles between
the ER and Golgi complex; involved in anterograde and
retrograde transport between the ER and Golgi;
synaptobrevin homolog'"
/codon_start=1
/product="Sec22p"
/protein_id="EWG84498.1"
/translation="MIKSTLIYREDGLPLCTSVDNENDPSLFEQKQKVKIVVSRLTPQ
SATEATLESGSFEIHYLKKSMVYYFVICESGYPRNLAFSYLNDIAQEFEHSFANEYPK
PTVRPYQFVNFDNFLQMTKKSYSDKKVQDNLDQLNQELVGVKQIMSKNIEDLLYRGDS
LDKMSDMSSSLKETSKRYRKSAQKINFDLLISQYAPIVIVAFFFVFLFWWIFLK"
gene 626023..627075
/gene="Dcs1"
/locus_tag="R008_L12046"
mRNA 626023..627075
/gene="Dcs1"
/locus_tag="R008_L12046"
/product="Dcs1p"
CDS 626023..627075
/gene="Dcs1"
/locus_tag="R008_L12046"
/note="Similar to YLR270W 'Non-essential hydrolase
involved in mRNA decapping, may function in a feedback
mechanism to regulate deadenylation, contains
pyrophosphatase activity and a HIT (histidine triad)
motif; interacts with neutral trehalase Nth1p'"
/codon_start=1
/product="Dcs1p"
/protein_id="EWG84499.1"
/translation="MSQLPTDFASLIKRFQFVSVLDSNPQTKVMSLLGTIDNKDAIIT
AEKTHFLFDETVRRPSQDGRSTPVLYNCENEYSCINGIQELKEITSNDIYYWGLSVIK
QDMESNPTAKLNLIWPATPIHIKKYEQQNFHLVRETPEMYKRIVQPYIEEMCNNGRLK
WVNNILYEGAESERVVYKDFSEENKDDGFLILPDMKWDGMNLDSLYLVAIVYRTDIKT
IRDLRYSDRQWLINLNNKIRSIVPGCYNYAVHPDELRILVHYQPSYYHFHIHIVNIKH
PGLGNSIAAGKAILLEDIIEMLNYLGPEGYMNKTITYAIGENHDLWKRGLEEELTKQL
ERDGIPKIPKIVNGFK"
gene 627575..628399
/locus_tag="R008_L12051"
mRNA 627575..628399
/locus_tag="R008_L12051"
/product="hypothetical protein"
CDS 627575..628399
/locus_tag="R008_L12051"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84500.1"
/translation="MEESKTKRKEDRIDLKNTPPQKKSKRDSTNDKTASTSLRSIMPR
GYKMMENMGYKEGETLGSNESALKEPIKVEINTKRRGIRAEKPDPSTMNDMQMSEQRF
IKRESEMKNNKRLKKIWYRIQKVAFEMMGDSDLYNPGEDPRDFNVLWRSYVMQLNEEV
AKNDLNNHSNNDNEDKNNMIPMVNESLEASPAIKGEKFGRPSTLIDCDTSIIGSRITK
DTELAELEELSIEKRITKLNIFLRSEKYYCFFCGIKYKDEGDLYEHCPGVNEDDHK"
gene complement(628510..632043)
/gene="Ycs4"
/locus_tag="R008_L12056"
mRNA complement(628510..632043)
/gene="Ycs4"
/locus_tag="R008_L12056"
/product="Ycs4p"
CDS complement(628510..632043)
/gene="Ycs4"
/locus_tag="R008_L12056"
/note="Similar to YLR272C 'Subunit of the condensin
complex; required for establishment and maintenance of
chromosome condensation, chromosome segregation, chromatin
binding of condensin, tRNA gene clustering at the
nucleolus, and silencing at the mating type locus'"
/codon_start=1
/product="Ycs4p"
/protein_id="EWG84501.1"
/translation="MSGFSLSEYLTKFQTTDRESYPRLQDPSRELNVIIDQLAVSPEQ
IDASPDSLEALIDLCHDFPHLTPKLQTQLSYLISSSLSNLSKDIKANLSSNVNFTEIG
GLIPQWKRHLEEYGYLIQVLLTFLQDELHKVSSQSTNLNRSAKNSKNDSANVELFKRD
CNQMENLLESITKLLEINLSKIFQTTPEKDLFIGLFTRPLFVLLEIEPVTKVSSLKMF
IQRILAMCVKNHGQSSSIQSSLMTNLTYFLHLSVFNAELLKLLNDEYNYPQLTEDILK
EISTRVFNAKDTTGPKAISNFLIKLSELSPGIMLRQMNLVITLLNNSSITLRCSVVEA
CGNIVAELAQDPQTMEHYKQQIAVLIELLEERFQDSNPYVRTKAIQGCSKICDLSSKF
NKSKAKFTSLAVRSLQDRSSLVRRNSVKLLSKLLLKHPFKAIHGSQLRLSEWEEYLKG
SESQLNSTLKKVESQETLNDTIERSLIEEEVEQDEGQCRTELEGSFNKSAELSRIENE
VENINTATNTSVLMKLKLMIVYYKDAISFIKEIHKSIELISNLLFSKNRNEVLESMDF
LVLADAFDIELSEFGIKKMLHLVWMKGTNDEGTSISVHLIECYKQLFLTAPDSCNMQE
KAAHIAKNLINLSIGASIADLASLEQLLGMMYEQKMIDQHVINILWAIYNSASKASMQ
KEQNVNNRDSEKGFSKEQIHGSIIILGMLSLADNEIALKGLESLLNIGLGAVGLKDLT
LCRYSCLALERMVPKRSTIITKAINQELEDVAVKKLYAIIINYTKDNEYYPMCEQALS
ALFTISSKPDILATDLIREKTMMTFGKPEEEDSILSLEQSSRVVSLSQLLFIVGQVAI
KTLVYLEKCEAEFKKRKIEAETRNGKVKNQGADVTNTTQDNGGDKELEMIGGTNEDDF
TDAIQFVKENELLFGEKSILGKFCPIVEEIVSNSSRFSDPMLQRTATLCLEKLMCLSS
KYCEKSLPLLITVMEKSPDPTIRSNAVLGLGDMAVCFNNLVDENTDYLYRRLHDENLM
VQRTCLMTVTFLILAGQVKVKGQLGEMAKCLDNPDQGISDMCRLFFTELASKDNAIYN
GFIDIFSNLSSDDLLGKESFKKIIKFLLTFIDKERHQKQLNEKLVGRLRKCETQKQWD
DIAFVLNNLPYKNEDVTALLEQGFKVASAKE"
assembly_gap 632381..632423
/estimated_length=43
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(632712..632785)
/locus_tag="R008_L12066"
tRNA complement(632712..632785)
/locus_tag="R008_L12066"
/product="tRNA-Val"
/note="TV(AAC)L"
assembly_gap 632801..632822
/estimated_length=22
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(633946..635892)
/gene="Pig1"
/locus_tag="R008_L12076"
mRNA complement(633946..635892)
/gene="Pig1"
/locus_tag="R008_L12076"
/product="Pig1p"
CDS complement(633946..635892)
/gene="Pig1"
/locus_tag="R008_L12076"
/note="Similar to YLR273C Putative targeting subunit for
the type-1 protein phosphatase Glc7p that tethers it to
the Gsy2p glycogen synthase"
/codon_start=1
/product="Pig1p"
/protein_id="EWG84502.1"
/translation="MPYSHGKKLKPSLKLAKTISTSSFVSSTTSNSFSPLEDSTSASS
STSSSSSGKSVRFAAHLYTVKKFNTKLAPISISEKAASNLTRNLHNNAIPLTFPFIRG
EDHRYSLDILDYSDLEYDNKDVEYDNESDVEDNAMLMHDRSMFIEKEILCFGEEETFD
MADWKLVSNNLNPFKSDCKVDVTELEDKIFKYLNGQNIKVHSLELSDPVSYEDICSNN
FGNCQIWGLIFVNNLNFEKKIEIKFTLNNWADIHYINAHYNKSVTPHVDEFKFIIDIS
ALKLNLISKNLIYANFFERKTTCLLNLQFCCRYDVNGFEYRSFYDNNDYKNYEITISL
SAINLNRAVSNSSIFNSNLGPSKMRASNAEVTMSKNNKNSKKPLRKFIKDTDYYNDSP
LKHKFYQSFETKAACKTEPVPQTFKAETIDCEIEPFNYFFEPPDSQTNEDMSDSSYDL
SLQDFNYWEFSNHGLGKALADSDILQFKNYPKPEPFSRPPIIDDTFTLNTDDRTLGLK
TEKLEDNLAKEWKSAKTRTTLNETPLHDDEHRTSFTYTTWNNSTDTLMKKQEERPVES
ASCSQLSIATIKAEEDLLYQDYINSGRESSSPEISPLNNTTSLPFFPGDNMSDSSGEY
EERISLSPNKIQIFRDYFYKSPSP"
gene 636418..638744
/gene="Mcm5"
/locus_tag="R008_L12081"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 639240..639662
/gene="Smd2"
/locus_tag="R008_L12086"
mRNA join(639240..639244,639335..639662)
/gene="Smd2"
/locus_tag="R008_L12086"
/product="Smd2p"
CDS join(639240..639244,639335..639662)
/gene="Smd2"
/locus_tag="R008_L12086"
/note="Similar to YLR275W 'Core Sm protein Sm D2; part of
heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p,
Smx3p, and Smx2p) that is part of the spliceosomal U1, U2,
U4, and U5 snRNPs; homolog of human Sm D2'"
/codon_start=1
/product="Smd2p"
/protein_id="EWG84503.1"
/translation="MSSQIIDRPKHELSRAELEELEEFEFKHGPMSLINDAMVTRTPV
IISLRNNHKIIARVKAFDRHCNMVLENVKELWTEKKGKNVINRERFISKLFLRGDSVI
VVLKTPVE"
gene complement(639908..641692)
/gene="Dbp9"
/locus_tag="R008_L12091"
mRNA complement(639908..641692)
/gene="Dbp9"
/locus_tag="R008_L12091"
/product="Dbp9p"
CDS complement(639908..641692)
/gene="Dbp9"
/locus_tag="R008_L12091"
/note="Similar to YLR276C ATP-dependent RNA helicase of
the DEAD-box family involved in biogenesis of the 60S
ribosomal subunit"
/codon_start=1
/product="Dbp9p"
/protein_id="EWG84504.1"
/translation="MSYEKKSVEGAYIDDSTTFEAFHLDSRLLQAIKNIGFQYPTLIQ
SHAIPLALQQKRDIIAKAATGSGKTLAYLIPVIETILEYKKTIDNGEENGTLGIILVP
TRELAQQVYNVLEKLVLYCSKDIRTLNISSDMSDSVLSTLLMDQPEIIVGTPGKLLDL
LQTKINSISLNELKFLVVDEVDLVLTFGYQDDLNKIGEYLPLKKNLQTFLMSATLNDD
IQALKQKFCRSPAILKFNDEEINKNQNKLLQYYVKVSEFDKFLLCYVIFKLNLIKGKT
LIFVNNIDRGYRLKLVMEQFGIKSCILNSELPVNSRQHIVDQFNKNVYQLLIATDDTE
YIKEEDDEIEEGHNTENQEEKSLEGEPENDKKPSKKKKVQVKKDKEYGVSRGVDFKNV
ACVLNFDLPTTAKSYVHRVGRTARGGKTGTAISFVVPLKEFGKHKPSMLQTAKKDERI
LSRIIKQQSKLGLELQPYKFDQKQVEAFRYRMEDGFRAVTQVAIREARVKELKQELLA
SEKLKRHFEENPKELQSLRHDKELHPARVQQHLKRVPDYLLPESARGNGTKVKFVPFH
NAKKRHSHKKGRVSKPKNGKVDPLKNFK"
gene complement(642023..644362)
/gene="Ysh1"
/locus_tag="R008_L12096"
mRNA complement(642023..644362)
/gene="Ysh1"
/locus_tag="R008_L12096"
/product="Ysh1p"
CDS complement(642023..644362)
/gene="Ysh1"
/locus_tag="R008_L12096"
/note="Similar to YLR277C 'Putative endoribonuclease,
subunit of the mRNA cleavage and polyadenylation
specificity complex; required for 3' processing, splicing,
and transcriptional termination of mRNAs and snoRNAs'"
/codon_start=1
/product="Ysh1p"
/protein_id="EWG84505.1"
/translation="MERTNTTTFKFFSLGGSNEVGRSCHILQYKGKTVMLDAGIHPAY
QGLASLPFYDEFDLSKVDILLISHFHLDHAASLPYVMQRTNFQGRVFMTHPTKAIYRW
LLRDFVRVTSIGSSSSSMGTKDEGLFSDEDLVDSFDKIETVDYHSTVDVNGIKFTAFH
AGHVLGAAMFQIEIAGLRVLFTGDYSREVDRHLNSAEVPPLSSNVLIVESTFGTATHE
PRLNRERKLTQLIHSTVMRGGRVLLPVFALGRAQEIMLILDEYWSQHADELGGGQVPI
FYASNLAKKCMSVFQTYVNMMNDDIRKKFRDSQTNPFIFKNISYLRNLEDFQDFGPSV
MLASPGMLQSGLSRDLLERWCPEDKNLVLITGYSIEGTMAKFIMLEPDTIPSINNPEI
TIPRRCQVEEISFAAHVDFQENLEFIEKISAPNIILVHGEANPMGRLKSALLSNFASL
KGTDNEVHVFNPRNCVEVDLEFQGVKVAKAVGNIVNEIYKEENVEIKEEIAAKIEPIK
EENEDNLDSQAEKGLVDEEEHKDIVVSGILVSDDKNFELDFLSLSDLREHHPDLSTTI
LRERQSVRVNCKKELIYWHILQMFGEAEVLQDDDRVTNQEPKVKEESKDNLTNTGKLI
LQIMGDIKLTIVNTLAVVEWTQDLMNDTVADSIIAILMNVDSAPASVKLSSHSCDDHD
HNNVQSNAQGKIDEVERVKQISRLFKEQFGDCFTLFLNKDEYASNKEETITGVVTIGK
STAKIDFNNMKILECNSNPLKGRVESLLNIGGNLVTPLC"
gene complement(644888..648900)
/locus_tag="R008_L12106"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 650054..650998
/locus_tag="R008_L12131"
mRNA 650054..650998
/locus_tag="R008_L12131"
/product="hypothetical protein"
CDS 650054..650998
/locus_tag="R008_L12131"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84506.1"
/translation="MIRLIRTLPLRCFKTRIRRQGSLLCLRCFSSYSKPLLQKSMSLK
NIQLSDLSSSPLSKNKEKQEKPEKENEGKHSIGLLDRFSEDFITQGNGLKPTTSQNQL
DTIKFYQMLRERGNFSDEQCKIIIALLLQLLNDQFYSCYNDLFLRDMELNKQSHLFSS
LETELKFAIQNSRDTQLNEHRLQLLKLKRELNSIHDELNEIIIDLLQKDAKLEFNNQK
LENTLLYRQLNLKLNDCSNKIQTKILGDIRSHIENLRWQTTRSGLLVILVLVCSIMIG
VSASKKERPGLQEPEEPEILAPKEDIDTTFPQDQHDID"
gene complement(651066..651908)
/gene="Eci1"
/locus_tag="R008_L12136"
mRNA complement(651066..651908)
/gene="Eci1"
/locus_tag="R008_L12136"
/product="Eci1p"
CDS complement(651066..651908)
/gene="Eci1"
/locus_tag="R008_L12136"
/note="Similar to YLR284C Peroxisomal
delta3,delta2-enoyl-CoA isomerase, hexameric protein that
converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential
for the beta-oxidation of unsaturated fatty acids,
oleate-induced"
/codon_start=1
/product="Eci1p"
/protein_id="EWG84507.1"
/translation="MSQEIRQNEKISYRIEGPFFIIHLINPDNLNALEGEDYIYLGEL
LELADRNRDVYFTIIQSSGRFFSSGADFKGIAKAQGDDTNKYPSETSKWVSNFVARNV
YVTDAFIKHSKVLICCLNGPAIGLSAALVALCDIVYSINDKVYLLYPFANLGLITEGG
TTVSLPLKFGTNTTYECLMFNKPFKYDIMCENGFISKNFNMPSSNAEAFNAKVLEELR
EKVKGLYLPSCLGMKKLLKSNHIDAFNKANSVEVNESLKYWVDGEPLKRFRQLGSKQR
KHRL"
gene 652228..653013
/gene="Nnt1"
/locus_tag="R008_L12141"
mRNA 652228..653013
/gene="Nnt1"
/locus_tag="R008_L12141"
/product="Nnt1p"
CDS 652228..653013
/gene="Nnt1"
/locus_tag="R008_L12141"
/note="Similar to YLR285W Putative nicotinamide
N-methyltransferase, has a role in rDNA silencing and in
lifespan determination"
/codon_start=1
/product="Nnt1p"
/protein_id="EWG84508.1"
/translation="MSDIESLGEAAGLFEEPEDFLPPPPKPHFAEYQRSHITKESKSD
VKDIKLRLVGTSPLWGHLLWNAGIYTANHLDSHPELIKGKTVLELGAAAALPSVICAL
NGAQMVVSTDYPDPDLMQNIDYNIKSNVPEGFNNVTTEGYIWGNDYSPLLAHIEKIGN
NNGKFDLIILSDLVFNHTEHHKLLQTTKDLLAEKGQALVVFSPHRPKLLEKDLEFFEL
AKNEFHLVPQLIEMVNWKPMFDEDEETIEVRSRVYAYYLTHEK"
gene complement(653036..653206)
/locus_tag="R008_L12146"
mRNA complement(653036..653206)
/locus_tag="R008_L12146"
/product="hypothetical protein"
CDS complement(653036..653206)
/locus_tag="R008_L12146"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84509.1"
/translation="MFLRFYNRLLYIYNELFNFLRFTVFLPFCEFVPHHDLFYLIVTY
QRTLVYKYKNDC"
gene complement(653316..654984)
/gene="Cts1"
/locus_tag="R008_L12151"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(655838..656906)
/locus_tag="R008_L12161"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 656698..656780
/estimated_length=83
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(657385..657990)
/gene="Rps30a"
/locus_tag="R008_L12166"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(658310..659734)
/gene="Mec3"
/locus_tag="R008_L12171"
mRNA complement(658310..659734)
/gene="Mec3"
/locus_tag="R008_L12171"
/product="Mec3p"
CDS complement(658310..659734)
/gene="Mec3"
/locus_tag="R008_L12171"
/note="Similar to YLR288C 'DNA damage and meiotic
pachytene checkpoint protein; subunit of a heterotrimeric
complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp,
loaded onto partial duplex DNA by a clamp loader complex;
homolog of human and S. pombe Hus1'"
/codon_start=1
/product="Mec3p"
/protein_id="EWG84510.1"
/translation="MKLKLIVNGCEAPDDYKLLRTTINTVASLRKTAILRFNSERLTI
ISTPKSSLNSSNNGTILRGDTGQLWCTIPHDVFRLYTVISARELNTITMECNCDSLLS
VFKRYDRVMNQGSSSNMIIKLQSMPEWNTNNGTLSGGTAGGVDTTSKPNPICALGITF
EEIVHTSGPNDAIVMNGGVDEHNGLPTTVGTGNLLASNKVIMHSFKVPVKLLFRAQDT
RIQEPMINYIQLMMYKLPPISGEFGSAFHGFIRRVERYSNVNHIHLMGVKKKEHGNEG
DDVELKIIVNELDWHLEICWNGPLDSVIQRQEGLTDNPSQNQHIDTDGRQEEGSLPII
EADKPMSSLYTNTRDREMEENIRYDEDLLRIEDSSIADTRGNIYTADTSGDTEFNDIS
VMVEKAEQESSSTHEVIIRCKDWKVCSKLYAAFEEVVLAISHDESCVFHCSLDRGSLE
DSEEVEKPRERGQIIYYIARSKGL"
gene 659919..661856
/gene="Guf1"
/locus_tag="R008_L12176"
mRNA 659919..661856
/gene="Guf1"
/locus_tag="R008_L12176"
/product="Guf1p"
CDS 659919..661856
/gene="Guf1"
/locus_tag="R008_L12176"
/note="Similar to YLR289W 'Mitochondrial matrix GTPase
that associates with mitochondrial ribosomes; important
for translation under temperature and nutrient stress; may
have a role in translational fidelity; similar to
bacterial LepA elongation factor'"
/codon_start=1
/product="Guf1p"
/protein_id="EWG84511.1"
/translation="MLKFRIRPVRHIRCYKRHAYFLRYNHTTTPAQKLQAQIEQIPLE
NYRNFSIVAHVDHGKSTLSDRLLEITHVIDPNARNKQVLDKLEVERERGITIKAQTCS
MFYKDKRTGKNYLLHLIDTPGHVDFRGEVSRSYASCGGAILLVDASQGIQAQTVANFY
LAFSLGLKLIPVINKIDLNFTDVKQVKDQIVNNFELPEEDIIGVSAKTGLNVEELLLP
AIIDRIPPPTGRPDKPFRALLVDSWYDAYLGAVLLVNIVDGFVRKNDKVICAQTKEKY
EVKDIGIMYPDRTSTGTLKTGQVGYLVLGMKDSKEAKIGDTIMHLSKVNETEVLPGFE
EQKPMVFVGAFPADGIEFKAMDDDMSRLVLNDRSVTLERETSNALGQGWRLGFLGSLH
ASVFRERLEKEYGSKLIITQPTVPYLVEFTDGKKKLITNPDEFPDGATKRVNVAAFHE
PFIEAVMTLPQEYLGSVIRLCDSNRGEQIDITYLNTNGQVMLKYYLPLSHLVDDFFGK
LKSVSRGFASLDYEDAGYRISDVVKLQLLVNGNAIDALSRVLHKSEVERVGREWVKKF
KEYVKSQLYEVVIQARANNKIIARETIKARRKDVLQKLHASDVSRRKKLLAKQKEGKK
HMKTVGNIQINQEAYQAFLRR"
gene complement(661974..662808)
/locus_tag="R008_L12181"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(663173..664318)
/gene="Gcd7"
/locus_tag="R008_L12186"
mRNA complement(663173..664318)
/gene="Gcd7"
/locus_tag="R008_L12186"
/product="Gcd7p"
CDS complement(663173..664318)
/gene="Gcd7"
/locus_tag="R008_L12186"
/note="Similar to YLR291C 'Beta subunit of the translation
initiation factor eIF2B, the guanine-nucleotide exchange
factor for eIF2; activity subsequently regulated by
phosphorylated eIF2; first identified as a negative
regulator of GCN4 expression'"
/codon_start=1
/product="Gcd7p"
/protein_id="EWG84512.1"
/translation="MSSQAFTSVHPNAATSDVNVTIDTFVAKLKRRQVQGSYAIALET
LQLLMRFISAARWNHVNDLIEQIRDLGNSLEKAHPTAFSCGNVIRRILAVLRDEVEED
TMSTTVTSTSVAEPLISSMFNLLQKPEQPHQNRKNSSGSSGMKTKTDYRQVAIQGIKD
LIDEIKNIDEGIQQIAIDLIHDHEILLTPTPDSKTVLKFLITARERSNRTFTVLVTEG
FPNNTKNAHEFAKKLAQHNIETLVVPDSAVFALMSRVGKVIIGTKAVFVNGGTISSNS
GVSSVCECAREFRTPVFAVAGLYKLSPLYPFDVEKFVEFGGSQRILPRMDPRKRLDTV
NQITDYVPPENIDIYITNVGGFNPSFIYRIAWDNYKQIDVHLDKNKA"
gene complement(664644..665225)
/gene="Sec72"
/locus_tag="R008_L12196"
mRNA complement(664644..665225)
/gene="Sec72"
/locus_tag="R008_L12196"
/product="Sec72p"
CDS complement(664644..665225)
/gene="Sec72"
/locus_tag="R008_L12196"
/note="Similar to YLR292C 'Non-essential subunit of Sec63
complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61
complex, Kar2p/BiP and Lhs1p forms a channel competent for
SRP-dependent and post-translational SRP-independent
protein targeting and import into the ER'"
/codon_start=1
/product="Sec72p"
/protein_id="EWG84513.1"
/translation="MVTLEYNANSKLITASDAVVALSTETNIDQINVLTTSLIGETNP
NFTPQPNEALSKMIKGLFESGMKNLQQKKLNEALKNVSLAIEMAQRKRAPWEAFAIQL
PELHFMLRSKIDLCLILGKHLEALQDLDFLLGTGLIQPDVFVRKADCLLKLKQWEEAR
ATCERGLALAPEDMKLRALLIETARNLAEYNGE"
gene complement(665626..666285)
/gene="Gsp1"
/locus_tag="R008_L12201"
mRNA complement(665626..666285)
/gene="Gsp1"
/locus_tag="R008_L12201"
/product="Gsp1p"
CDS complement(665626..666285)
/gene="Gsp1"
/locus_tag="R008_L12201"
/note="Similar to YLR293C 'Ran GTPase, GTP binding protein
(mammalian Ranp homolog) involved in the maintenance of
nuclear organization, RNA processing and transport;
regulated by Srm1p, Rna1p, Yrb1p, Yrb2p, Yrp4p, Yrb30p,
Cse1p and Kap95p; yeast Gsp2p homolog'"
/codon_start=1
/product="Gsp1p"
/protein_id="EWG84514.1"
/translation="MSAPAANGEVPTFKLVLVGDGGTGKTTFVKRHLTGEFEKKYIAT
IGVEVHPLSFYTNFGEIKFDVWDTAGQEKFGGLRDGYYINAQCAIIMFDVTSRITYKN
VPNWHRDLVRVCENIPIVLCGNKVDVKERKVKAKTITFHRKKNLQYYDISAKSNYNFE
KPFLWLARKLAGNPQLEFVASPALAPPEVQVDEQLMQQYQQEMEQATALPLPDEDDAD
L"
gene complement(666854..667228)
/gene="Atp14"
/locus_tag="R008_L12216"
mRNA complement(666854..667228)
/gene="Atp14"
/locus_tag="R008_L12216"
/product="Atp14p"
CDS complement(666854..667228)
/gene="Atp14"
/locus_tag="R008_L12216"
/note="Similar to YLR295C Subunit h of the F0 sector of
mitochondrial F1F0 ATP synthase, which is a large,
evolutionarily conserved enzyme complex required for ATP
synthesis"
/codon_start=1
/product="Atp14p"
/protein_id="EWG84515.1"
/translation="MFPIASRRILLNASVLPLRLCNRNFTTTRISYNVIQDLYLRELK
DTKLAPSTLQDAEGNVKPWNPPQKPNLPELELQGPEALKAYTEQNVETAHVAKETEEG
ESEPIEEDWLVLDDAEETKESH"
gene 668897..669286
/locus_tag="R008_L12236"
mRNA 668897..669286
/locus_tag="R008_L12236"
/product="hypothetical protein"
CDS 668897..669286
/locus_tag="R008_L12236"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84516.1"
/translation="MVEGDFVDEQSNIALLSSKSMCGDHHSVKDSIGDEIFKLLTKIL
NSDEKASGDVHTLVSGTRDLSNFNLDNEPLENILAVFIISFIIVVVGVLLLGLIGMIF
ISLRSGSSNDKKLQSNDEEKQALAEKA"
gene complement(669570..670672)
/gene="Mas1"
/locus_tag="R008_L12241"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 670917..672899
/gene="Ecm38"
/locus_tag="R008_L12256"
mRNA 670917..672899
/gene="Ecm38"
/locus_tag="R008_L12256"
/product="Ecm38p"
CDS 670917..672899
/gene="Ecm38"
/locus_tag="R008_L12256"
/note="Similar to YLR299W 'Gamma-glutamyltranspeptidase,
major glutathione-degrading enzyme; involved in
detoxification of electrophilic xenobiotics; expression
induced mainly by nitrogen starvation'"
/codon_start=1
/product="Ecm38p"
/protein_id="EWG84517.1"
/translation="MLLCNRKVPKTLNTCFILHIFTLLTLGVLVSGMPSKMVSFASQE
TLQRINNLLRGSANRDVDIIAEYLKKDDDDDGGDKDHHNIDIDPLPRRPSLTPDRQLL
KVGLHGAISSDLEVCSNLTINEVLLKFPGSNAADAAVTQALCKGMVNFFNSGIGGGGY
VVFSGKDDEDRLSIDFREKAPMDSHKFMFENCSLCSKIGGLAVGVPGELMGLYRLFKE
RGSGQVDWRDLIEPVAKLGSVGWQIGEALGATLELYEDVFLTLKEDWSFVLNSTHDGV
LKEGDWIKRPALSNMLMELAKNGSVAPFYDPDHWIAKSMIDTVAKYNGIMNLQDVSSY
DVHVTKPLSMKIRKGANFIPDNDMTVLTSSGSSSGAALLATLRIMDNFQNQEGGDYEK
ETTYHLLESMKWMASARSRLGDFEGEALPKHIEEVLDPEWALKAVKSIKRNSQDGNFK
TLENWTLYDPAYDINNPHGTAHFSIVDSHGNAVSLTTTINLLFGSLVHDPKTGVIFNN
EMDDFAQFNKSNSFELAPSIYNFPEPGKRPLSSTAPTIVLSELGIPDLVVGASGGSRI
TTSVLQTIVRTYWYNMPILETIAYPRIHHQLLPDRIELESFPMIGKAVLSTLKEIGYT
MKEVFPKSVVNAIRNVRGEWHAVSDYWRKRGISSVY"
gene 673803..675149
/gene="Exg1"
/locus_tag="R008_L12266"
mRNA 673803..675149
/gene="Exg1"
/locus_tag="R008_L12266"
/product="Exg1p"
CDS 673803..675149
/gene="Exg1"
/locus_tag="R008_L12266"
/note="Similar to YLR300W 'Major exo-1,3-beta-glucanase of
the cell wall, involved in cell wall beta-glucan assembly;
exists as three differentially glycosylated isoenzymes'"
/codon_start=1
/product="Exg1p"
/protein_id="EWG84518.1"
/translation="MLSLKTLLCTLLTVSSVLATPVPARDPSSIQFVHEENKKRYYDY
DHGSLGEPIRGVNIGGWLLLEPYITPSLFEAFRTNDDNDEGIPVDEYHFCQYLGKDLA
KSRLQSHWSTFYQEQDFANIASQGFNLVRIPIGYWAFQTLDDDPYVSGLQESYLDQAI
GWARNNSLKVWVDLHGAAGSQNGFDNSGLRDSYKFLEDSNLAVTTNVLNYILKKYSAE
EYLDTVIGIELINEPLGPVLDMDKMKNDYLAPAYEYLRNNIKSDQVIIIHDAFQPYNY
WDDFMTENDGYWGVTIDHHHYQVFASDQLERSIDEHIKVACEWGTGVLNESHWTVCGE
FAAALTDCTKWLNSVGFGARYDGSWVNGDQTSSYIGSCANNDDIAYWSDERKENTRRY
VEAQLDAFEMRGGWIIWCYKTESSLEWDAQRLMFNGLFPQPLTDRKYPNQCGTISN"
gene 675673..676407
/locus_tag="R008_L12271"
mRNA 675673..676407
/locus_tag="R008_L12271"
/product="hypothetical protein"
CDS 675673..676407
/locus_tag="R008_L12271"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84519.1"
/translation="MPALLKRLLFQVGPHPNERTFTLSSVSTDGHYISLRPFVKPSGD
ELSFPFEWAFAGTNETVKVNDQGNGVVTQDFNFWLDTNVYLNVPNTHRGEVNTTWKNW
DSGCVEETGAVYPFGADKESVSFRELWQPVDPSREDLVIVSPNNEKFSSNARSIVLKV
TDEAYDGLVIVIGRWIQGFLSQKNNNTIEGLNFIRLLEKDSGKSEFLLSYGKEVNKIP
QSYENLKKGSTVTSNGLNWEVIEYHA"
gene 676600..676700
/locus_tag="R008_L12276"
tRNA 676600..676700
/locus_tag="R008_L12276"
/product="tRNA-Leu"
/note="TL(UAG)L2"
gene 677053..678387
/gene="Met17"
/locus_tag="R008_L12286"
mRNA 677053..678387
/gene="Met17"
/locus_tag="R008_L12286"
/product="Met17p"
CDS 677053..678387
/gene="Met17"
/locus_tag="R008_L12286"
/note="Similar to YLR303W Methionine and cysteine synthase
(O-acetyl homoserine-O-acetyl serine sulfhydrylase),
required for sulfur amino acid synthesis"
/codon_start=1
/product="Met17p"
/protein_id="EWG84520.1"
/translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGS
QLFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGD
NIVSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPK
YNVPDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTI
GGIIVDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPL
MNPFASFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAK
KYLSNGFGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAP
YFTTHKQLNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
assembly_gap 678538..681919
/estimated_length=3382
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 684853..685983
/estimated_length=1131
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(686324..688660)
/gene="Aco1"
/locus_tag="R008_L12291"
mRNA complement(686324..688660)
/gene="Aco1"
/locus_tag="R008_L12291"
/product="Aco1p"
CDS complement(686324..688660)
/gene="Aco1"
/locus_tag="R008_L12291"
/note="Similar to YLR304C 'Aconitase, required for the
tricarboxylic acid (TCA) cycle and also independently
required for mitochondrial genome maintenance;
phosphorylated; component of the mitochondrial nucleoid;
mutation leads to glutamate auxotrophy'"
/codon_start=1
/product="Aco1p"
/protein_id="EWG84521.1"
/translation="MLSARSAIKRPIVRGLATVSNLTRDSKVNQNLLEDHSFINYKQN
VETLDIVRKRLNRPFTYAEKILYGHLDDPHGQDIQRGVSYLKLRPDRVACQDATAQMA
ILQFMSAGLPQVAKPVTVHCDHLIQAQVGGEKDLKRAIDLNKEVYDFLASATAKYNMG
FWKPGSGIIHQIVLENYAFPGALIIGTDSHTPNAGGLGQLAIGVGGADAVDVMAGRPW
ELKAPKILGVKLTGKMNGWTSPKDIILKLAGITTVKGGTGKIVEYFGDGVDTFSATGM
GTICNMGAEIGATTSVFPFNKSMIEYLEATGRGKIADFAKLYHKDLLSADKDAEYDEV
VEIDLNTLEPYINGPFTPDLATPVSKMKEVAVANNWPLDVRVGLIGSCTNSSYEDMSR
SASIVKDAAAHGLKSKTIFTVTPGSEQIRATIERDGQLETFKEFGGIVLANACGPCIG
QWDRRDIKKGDKNTIVSSYNRNFTSRNDGNPQTHAFVASPELVTAFAIAGDLRFNPLT
DKLKDKDGNEFMLKPPHGDGLPQRGYDAGENTYQAPPADRSTVEVKVSPTSDRLQLLK
PFKPWDGKDAKDMPILIKAVGKTTTDHISMAGPWLKYRGHLENISNNYMIGAINAENK
KANCVKNVYTGEYKGVPDTARDYRDQGIKWVVIGDENFGEGSSREHAALEPRFLGGFA
IITKSFARIHETNLKKQGLLPLNFKNPADYDKINPDDRIDILGLAELAPGKPVTMRVH
PKNGKPWDAVLTHTFNDEQIEWFKYGSALNKIKADEKK"
gene complement(689273..694975)
/gene="Stt4"
/locus_tag="R008_L12296"
mRNA complement(689273..694975)
/gene="Stt4"
/locus_tag="R008_L12296"
/product="Stt4p"
CDS complement(689273..694975)
/gene="Stt4"
/locus_tag="R008_L12296"
/note="Similar to YLR305C 'Phosphatidylinositol-4-kinase
that functions in the Pkc1p protein kinase pathway;
required for normal vacuole morphology, cell wall
integrity, and actin cytoskeleton organization'"
/codon_start=1
/product="Stt4p"
/protein_id="EWG84522.1"
/translation="MRFTRGLKASSSLRAKAIGRLTKLSTGAPNDQNNNGTTLDLITH
TLPIFYSTNTSKIYTIPLTLSEWEVLTSLCVAIPTTLDLVETMLKEIIAPYFLETPRQ
RISDVLSSKFKLEQMRNPIELLTFQLTKFMIQACEQYPVLYENIGGIISTYFERVLKI
FTIKQSGLLSLVGFINAFIQFPNSTELTKFTWKKLAKLVLRGSFLNEVDKILNSSATF
TNDSIVQYYDAGNELSSAYLLELISRLQVSLISHLLNTSHVGANLSEFLLNQQYQFYK
FDQEVADVNDDTKCIDDFFFNVRSNKQFFTDMCKISLQFCSESHILDLSTDNRARFSF
DTRAHYLQTLCLIPFIEDTESELFESFTNVVSESIDKFFLSDVVTPSLIKAIVASASL
LNFFTEKLSLTLIRMFPLLVASPHITTETVNDVAKIFTTGLYPLNEDAIVSTIYSMNN
LLAVSEDGSPVPVLRERQLTITSGKNIEKDYFPLRNSSASLDGTGALLGNTTVGQLSS
HDVNSGATMTYHASLISNCVAATTTIASYYNTQSITALTISILTQKVNSMSKELDSVI
LNSLARLAPNTSLTEFSLLLKFFKSRTVIAAKIDDSALLKNIIKAKCVISKELLARHF
SSDLYFMYLHDLLDSIIASGEVERLEHHRPQTEISRVADQIATYLEPLAALLPVPGDT
PLDINKDEVTTNKFRNAWFNFVIHGYHLGGPIVKRNFSFLLTIAYNSPPLASEFPANN
KELSLEMNTILRRGSSNENIKQQKQQITEYFNTNIVQYRTTSSSKIMFLAAAVLLETI
RCEAGDCSKTLLYFSDPSILSGSIEKCIAVLSVSMIRKYARLIQKGNDAIFNSKMIAQ
QLNNLLLCLSHREPTLQDAAFHACEIFIRSIPSSLCHHLSLYTLLDMLTALFDSILDS
EAHKFEPRYEFKLKHSKTTILVPSSSSWRATTLSRLHKSAKEWVRILLNRSNQDTKIL
LQSYISDLGEYSRLNSVEFGVSFAMDMAGLILPADKELSRLTYYGPEKPNTISGFISL
HSWRSKYLFDTAITSSPEDIKRQIGISTQNIRKNLTLGNKIITKDVTDFLDMATALLI
LGNGAPASLIYDIVHIPFEVFTSASLKIATNVWLTIITEKPEVAHLLLVEVCYCWMRS
IDDNIGLYSRDHDLKGEEYQKMEYSPYDKAGINRDAKNASQAMQPHLHVIKFFASHFE
GTLFQSDFLLKIFTKCALYGIKNLYKASLHPFARMIRHELLLFATLVLNASYKQGSKY
MGRLSQEITNGALSWFKRPVAWPFGSNELKIKADLSVTRDLFLQLNKLSSLMSRHCGK
DYKILNYFLASEIQQIQTWLTPTEKIEGADSNELTSDIVEATFAKDPTLAINLLQRCY
SKKAEDVLVGLVAKHALMCVGSPSALDLFIKGSHLGSKKDLHATLYWAPVSPLKSINL
FLPEWQGNSFILQFSIYSLESQDVNLAFFYVPQIVQCLRYDKTGYVERLILDTAKISV
LFSHQIIWNMLANCYKDDEGIQEDEIKPTLDRIRERMVSSFSQSHRDFYEREFEFFDE
VTGISGKLKPYIKKSKAEKKHKIDEEMSKIEVKPDVYLPSNPDGVVIDIDRKGGKPLQ
SHAKAPFMATFKIKKDVKDPLTGKNKEVEKWQAAIFKVGDDCRQDVLALQLISLFRTI
WSSIGLDVYVFPYRVTATAPGCGVIDVLPNSVSRDMLGREAVNGLYEYFTSKFGNEST
IEFQNARNNFVKSLAGYSVISYLLQFKDRHNGNIMYDDQGHCLHIDFGFIFDIVPGGI
KFEAVPFKLTKEMVKVMGGSPQTPAYLDFEELCIKAYLAARPHVEAIIECVNPMLGSG
LPCFKGHKTIRNLRARFQPQKTDHEAALYMKALIRKSYESIFTKGYDEFQRLTNGIPY
"
gene 695264..695964
/gene="Ubc12"
/locus_tag="R008_L12301"
mRNA join(695264..695266,695401..695964)
/gene="Ubc12"
/locus_tag="R008_L12301"
/product="Ubc12p"
CDS join(695264..695266,695401..695964)
/gene="Ubc12"
/locus_tag="R008_L12301"
/note="Similar to YLR306W 'Enzyme that mediates the
conjugation of Rub1p, a ubiquitin-like protein, to other
proteins; related to E2 ubiquitin-conjugating enzymes'"
/codon_start=1
/product="Ubc12p"
/protein_id="EWG84523.1"
/translation="MLKLRQLQKKKQKENENSSSIQPNLSAARIRLKRDLDSLDLPPT
VTLNVITSPDSADRSQSPKLEVIVRPDEGYYNYGSINFNLDFNEVYPIEPPKVVCLKK
IFHPNIDLKGNVCLNILREDWSPALDLQSIITGLLFLFLEPNPNDPLNKDAAKLLCEG
EKEFAEAVRLTMSGGSIEHVKYDNIVSP"
gene 696732..697639
/gene="Cda1"
/locus_tag="R008_L12306"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 696785..696825
/estimated_length=41
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(697962..698225)
/locus_tag="R008_L12311"
mRNA complement(697962..698225)
/locus_tag="R008_L12311"
/product="hypothetical protein"
CDS complement(697962..698225)
/locus_tag="R008_L12311"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84524.1"
/translation="MSLRNISMITKNLQTTAKCYVPKSSPTSTTIPVIRDASTTQCRR
ITTVINITSLKGYSPSPRTVHDKPIVICTDNEEVETVSEHVKV"
gene 699051..699989
/gene="Cda2"
/locus_tag="R008_L12316"
mRNA 699051..699989
/gene="Cda2"
/locus_tag="R008_L12316"
/product="Cda2p"
CDS 699051..699989
/gene="Cda2"
/locus_tag="R008_L12316"
/note="Similar to YLR308W 'Chitin deacetylase, together
with Cda1p involved in the biosynthesis ascospore wall
component, chitosan; required for proper rigidity of the
ascospore wall'"
/codon_start=1
/product="Cda2p"
/protein_id="EWG84525.1"
/translation="MRIQLNTIDLQCIIALSCLGQFVHAEANREDLKQIDFQFPVLER
AATKTPFPDWLSAFTGLKEWPGLDPPYIPLDFIDFSQIPDYKEYDQNHCDSVPRDSCS
FDCHHCTEHDDVYTCSKLSQTFDDGPSASTTKLLDRLKHNSTFFNLGVNIVQHPDIYH
RMQKEGHLIGSHTWSHVYLPNVSNEKIIAQIEWSIWAMNATGNHTPKWFRPPYGGIDN
RVRAITRQFGLQAVLWDHDTFDWSLLLNDSVITEQEILQNVINWNKSGSGLILEHDST
EKTVDLAIKINKLIGDDQSTVSHCVGGIDYIKEFLS"
gene complement(700147..702881)
/gene="Imh1"
/locus_tag="R008_L12321"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(703336..708105)
/gene="Cdc25"
/locus_tag="R008_L12326"
mRNA complement(703336..708105)
/gene="Cdc25"
/locus_tag="R008_L12326"
/product="Cdc25p"
CDS complement(703336..708105)
/gene="Cdc25"
/locus_tag="R008_L12326"
/note="Similar to YLR310C 'Membrane bound guanine
nucleotide exchange factor (GEF or GDP-release factor);
indirectly regulates adenylate cyclase through activation
of Ras1p and Ras2p by stimulating the exchange of GDP for
GTP; required for progression through G1'"
/codon_start=1
/product="Cdc25p"
/protein_id="EWG84526.1"
/translation="MSDTNTSIPNTSSAREAGNASQTPSISSSSNTSTTTNTESSSAS
LSSSPSTSELTSIRPIGIVVAAYDFNYPIKKDSSSQLLSVQQGETIYILNKNSSGWWD
GLVIDDSNGKVNRGWFPQNFGRPLRDSHLRKHSHPMKKYSSSKSSRRSSLNSLGNSAY
LHVPRNPSKSRRGSSTLSASLSNAHNAETSSGHNNTVSMNNSPFSAPNDASHITPQSS
NFNSNASLSQDMTKSADGSSEMNTNAIMNNNETNLQTSGEKAGPPLVAEETIKILPLE
EIGMIINGIHSNIASTWSPIPLITKTSDYKLVYYNKDLDIYCSELPLISNSIMESDDI
CDSEPKFPPNDHLVNLYTRDLRKNANIEDSSTRSKQSESEQNRSSLLMEKQDSKETDG
NNNSINDDDNNNENNKNEFNEAGPSSLNSLSAPDLTQNIQSRVVAPSRSSILAKSDIF
YHYSRDIKLWTELQDLTVYYTKTAHKMFLKKNRLNFTKYFDLISDSIVFTQLGCRLMQ
HEIKAKSCSKEIKKIFKGLISSLSRISINSHLYFDSAFHRKKMDTMNDKDNDNQENNC
SRTEGDDGKIEVDGVHDLVSVPLSGKRNVSTSTTDTLTPMRSSFSTVNENDMENFSVL
GPRNSVNSVVTPRTSIQNSTLEDFSPSNKNFKSAKSIYEMVDVEFSKFLRHVQLLYFV
LQSSVFSDDNTLPQLLPRFFKGSFSGGSWTNPFSTFITDEFGNATKNKAVTSNEVTAS
SSKNSSISRIPPKMADAIASASGYSANSETNSQIDLKASSAASGSVFTPFNRPSHNRT
FSRARVSKRKKKYPLTVDTLNTMKKKSSQIFEKLNNATGEHLKIISKPKSRIRNLEIN
SSTYEQINQNVLLLEILENLDLSIFINLKNLIKTPSILLDLESEEFLVHAMSSVSSVL
TEFFDIKQAFHDIVIRLIMTTQQTTLDDPYLFSSMRSNFPVGHHEPFKNISNTPLVKG
PFHKKNEQLALSLFHVLVSQDVEFNNLEFLNNSDDFKDACEKYVEISNLACIIVDQLI
EERENLLNYAARMMKNNLTAELLKGEQEKWFDIYSEDYSDDDSENDEAIIDDELGSED
YIERKAANIEKNLPWFLTSDYETSLVYDSRGKIRGGTKEALIEHLTSHELVDAAFNVT
MLITFRSILTAREFFYALIYRYNLYPPEGLSYDDYNIWIEKKSNPIKCRVVNIMRTFL
TQYWTRNYYEPGIPLILNFAKMVVSEKIPGAEDLLQKINEKLINENEKEPVDPKQQDS
VSAVVQTTKRDNKSPIHMSSSSLPSSASSAFFRLKKLKLLDIDPYTYATQLTVLEHDL
YLRITMFECLDRAWGTKYCNMGGSPNITKFIANANTLTNFVSHTIVKQADVKTRSKLT
QYFVTVAQHCKELNNFSSMTAIVSALYSSPIYRLKKTWDLVSTESKDLLKNLNNLMDS
KRNFVKYRELLRSVTDVACVPFFGVYLSDLTFTFVGNPDFLHNSTNIINFSKRTKIAN
IVEEIISFKRFHYKLKRLDDIQTVIEASLENVPHIEKQYQLSLQVEPRSGNTKGSTHA
SSASGTKTAKFLSEFTDDKNGNFLKLNKKKPPSRLFR"
assembly_gap 708437..708503
/estimated_length=67
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(708665..709861)
/gene="Qnq1"
/locus_tag="R008_L12336"
mRNA complement(708665..709861)
/gene="Qnq1"
/locus_tag="R008_L12336"
/product="Qnq1p"
CDS complement(708665..709861)
/gene="Qnq1"
/locus_tag="R008_L12336"
/note="Similar to YLR312C Putative protein of unknown
function"
/codon_start=1
/product="Qnq1p"
/protein_id="EWG84527.1"
/translation="MSEEDDHWNLVRLRRLRKGREGEEQSSKSEISLDSLHESSFAGE
DDEDFDADVLSNTSSEESAQMNRIYDFRTSNEFSNVGVNIDQTGVPTISESFDTLSGS
NIGGTVLPSMEGSKLKDSTIRNSSSRSDHIIDKSEGKSAKLKMWHVIMLSSLLSMTFS
YLALEYSLTGDVLAGFKSQQSLRNNERKLLYGNIDFVDKKSYDSSSDSLSQWAPSGKY
YVDFDNHIAYPLKDDDLMGWRRYKTDLVILWYITKARMKDGWHKRINKINGGRIKLHL
FLKNSFKSAQESLRVLHKEQKRRWKRLFVLLHNKYRQFSPHIKRYFDHSCQKAKQCWS
GSRLHLRKLRFKSMKPFRVFQFKVRKDTNWFVKQLKRFGLKLQHSRMYKAMSECRKKN
YFKCKH"
gene 710507..711268
/gene="Mrpl15"
/locus_tag="R008_L12341"
mRNA 710507..711268
/gene="Mrpl15"
/locus_tag="R008_L12341"
/product="Mrpl15p"
CDS 710507..711268
/gene="Mrpl15"
/locus_tag="R008_L12341"
/note="Similar to YLR312W-A no_gene_description"
/codon_start=1
/product="Mrpl15p"
/protein_id="EWG84528.1"
/translation="MENSMMFISRSLRRPVTALNCNLQSVRTVIYLHKGPRINGLRRD
PESYLRNPSGVLFTEVNAKECQDKVRSILQLPKYGINLSNELILQCLTHKSFAHGSKP
YNEKLNLLGAQFLKLQTCIHSLKNGSPAESCENGQLSLQFSNLGTKFAKELTSKNTAC
TFVKLHNLGPFIFWKMRDPIKDGHINGETTIFASVLNAFIGAILSTNGSEKAAKFIQG
SLLDKEDLHSLVNIANENVASAKAKISDKENKAFL"
gene complement(711383..713368)
/gene="Sph1"
/locus_tag="R008_L12346"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(713601..715163)
/gene="Cdc3"
/locus_tag="R008_L12351"
mRNA complement(713601..715163)
/gene="Cdc3"
/locus_tag="R008_L12351"
/product="Cdc3p"
CDS complement(713601..715163)
/gene="Cdc3"
/locus_tag="R008_L12351"
/note="Similar to YLR314C 'Component of the septin ring of
the mother-bud neck that is required for cytokinesis;
septins recruit proteins to the neck and can act as a
barrier to diffusion at the membrane, and they comprise
the 10nm filaments seen with EM'"
/codon_start=1
/product="Cdc3p"
/protein_id="EWG84529.1"
/translation="MSLKEEQVSIKQDPEQEERQHDQFNDVQIKQESQDHDGVDSQYT
NGTQNDDSERFEAAESDVKVEPGLGMGITSSQSEKGQVLPDQPEIKFIRRQINGYVGF
ANLPKQWHRRSIKNGFSFNLLCVGPDGIGKTTLMKTLFNNDDIEANLVKDYEEELAND
QEEEEGQGEGHENQSQEQRHKVKIKSYESVIEENGVKLNLNVIDTEGFGDFLNNDQKS
WDPIIKEIDSRFDQYLDAENKINRHSINDKRIHACLYFIEPTGHYLKPLDLKFMQSVY
EKCNLIPVIAKSDILTDEEILSFKKTIMNQLIQSNIELFKPPIYSNDDAENSHLSERL
FSSLPYAVIGSNDIVENYSGNQVRGRSYPWGVIEVDNDNHSDFNLLKNLLIKQFMEEL
KERTSKILYENYRSSKLAKLGIKQDNSVFKEFDPISKQQEEKTLHEAKLAKLEIEMKT
VFQQKVSEKEKKLQKSETELFARHKEMKEKLTKQLKALEDKKKQLELSINSASPNVNH
SPVPTKKKGFLR"
gene 715834..716295
/gene="Nkp2"
/locus_tag="R008_L12356"
mRNA 715834..716295
/gene="Nkp2"
/locus_tag="R008_L12356"
/product="Nkp2p"
CDS 715834..716295
/gene="Nkp2"
/locus_tag="R008_L12356"
/note="Similar to YLR315W Non-essential kinetochore
protein, subunit of the Ctf19 central kinetochore complex
(Ctf19p-Mcm21p-Okp1p-Mcm22p-Mcm16p-Ctf3p-Chl4p-Mcm19p-
Nkp1p-Nkp2p-Ame1p-Mtw1p)"
/codon_start=1
/product="Nkp2p"
/protein_id="EWG84530.1"
/translation="MNSEQLLHNYVSDSLLTTLISFQEFKQQLQSYTSDEQQLQHWYE
LLQARDARVTSELEARIKQFFITLRSRLLRFLESEQLSHSLSLETLIDALYKINDLLQ
QRLQILDDAIQEKTSELAEFENMVRSPSAGDNAIPGLLQIIQSYINLLEEN"
gene complement(716292..717383)
/gene="Tad3"
/locus_tag="R008_L12361"
mRNA complement(join(716292..717099,717155..717206,
717275..717383))
/gene="Tad3"
/locus_tag="R008_L12361"
/product="Tad3p"
CDS complement(join(716292..717099,717155..717206,
717275..717383))
/gene="Tad3"
/locus_tag="R008_L12361"
/note="Similar to YLR316C Subunit of tRNA-specific
adenosine-34 deaminase, forms a heterodimer with Tad2p
that converts adenosine to inosine at the wobble position
of several tRNAs"
/codon_start=1
/product="Tad3p"
/protein_id="EWG84531.1"
/translation="MVKKVNNPLKIDYQNGIIENRLLQIRNFKDVNTPKLINVWSIRI
DPRDSKKVIELIRNDFQKNDPISLRHLKRIRKNIETSTLEVVLCSKEYICDEGEINNK
LKNIWVGTKKYELSDEIEVPEFAPSTKELNNAWSVKYWPLIWNGNPNDQILNDYKIDM
QEVRNELSRASTLSVKMATAGKQFPMVSVFVDPSRKKDKVVAEDGRNCENSLPIDHSV
MVGIRAVGERLREGVDEDANSYLCLDYDVYLTHEPCSMCSMALIHSRVRRVVFLTEMQ
RTGSLKLTSGDGYCMNDNKQLNSTYEAFQWIGEEYPVGQVDRDVCC"
gene 717562..720209
/gene="Est2"
/locus_tag="R008_L12371"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(720335..722701)
/gene="Bud6"
/locus_tag="R008_L12376"
mRNA complement(720335..722701)
/gene="Bud6"
/locus_tag="R008_L12376"
/product="Bud6p"
CDS complement(720335..722701)
/gene="Bud6"
/locus_tag="R008_L12376"
/note="Similar to YLR319C 'Actin- and formin-interacting
protein, involved in actin cable nucleation and polarized
cell growth; isolated as bipolar budding mutant; potential
Cdc28p substrate'"
/codon_start=1
/product="Bud6p"
/protein_id="EWG84532.1"
/translation="MKMAVDDPTYGTPKIKRTASSSSSIETTVTKLLMSTKHLLQVLT
QWSKGTTSGRLVSDAYVQLGNDFKVVSKFFMHAKVDMSDVGDVPMALRRVLEVTLREP
PSDETLNKHLPKIREIIVTLLDKLKVKQAILKNMQQEHRISVKSHHQQNPSFTSNLSL
GSEGTREGTPLSSRKSSIVRDQRQSDSVENSYGEKVNSTSTGTPSAQSAEATLTKPRT
NIKQNLKSNNAPNASDDDDALSQLKKGTNLQRRASKRYSAYHMAKLTNQSTTEAAAAA
GLMITPSPSMLHLEETVRKSKLHGNNNNDDDRNINSAENKGKSIDDVSKASPLAKTPL
PIENVRASPRRLSSVVTISPDKAMNGTCPVFLRIGDKTKKCHVQLPTTKNVLRLLFIE
RFAYSPGANSFPDIYIMDPQYGVFYELEELNLLDIKEGFVIELKLEENSNNTIKEFID
TVKIEISNSQNDIIRHLKEMSFGSAISGKQTEVLPQPGLEANKHDLVGQNKKDDDKTI
KDIQYELGKIKQVHNINRSNINETIFNILRKVDNFKSLSFSAKNSSNRMYMEKSQTEL
GDLSDTLLSKVDDLQDVIEIMRKDVAERRSQPAKKKLETVSKDLENAQADVLKLQEFI
DTEKPHWKKTWEAELDKVCEEQQFLTLQEELILDLKEDLGKALETFDLIKLCCEEQEK
NPSRSKSNPILPIMRPGTFNQVREQVMVAVQSLNPDHDSRVEAIDKAEKMWEMERKLK
ASNEFDDELENFVGNSNLKKSGGFEEVERIRKQKDEANLRAYFGPGFT"
gene 722961..727325
/gene="Mms22"
/locus_tag="R008_L12381"
mRNA 722961..727325
/gene="Mms22"
/locus_tag="R008_L12381"
/product="Mms22p"
CDS 722961..727325
/gene="Mms22"
/locus_tag="R008_L12381"
/note="Similar to YLR320W 'Protein that acts with Mms1p in
a repair pathway that may be involved in resolving
replication intermediates or preventing the damage caused
by blocked replication forks; required for accurate
meiotic chromosome segregation'"
/codon_start=1
/product="Mms22p"
/protein_id="EWG84533.1"
/translation="MDVDEPNPIVISDSEATDEEISIIYEPEFNENYLWAEENVQEAS
RSQKIVIERLSLDSTAGESCTPSVVTDTQVTTGLRWSLRKRKAIQKMPYSLERIKHRQ
LLEGYDISSFDSISNQLTLPKNASTVIHSNDILLTKRTGKPLDEQKDVTIDSIKPENS
SVQSQRYDSDEEIPKKRHRTFKDLDQDIVFQSGDSTEDEQDLASTNLQNTQNDEVIFR
GRVLNVRTGYRGVLPRVAWEKSLQKQQSSKVTKRKTQLLNHKGVAKRKMNRSAHIEDE
EQNLLNDLIAPDDELDIEENAPPDIYLGNLPEDREANEKELKELQEYYESKYSEDAQS
AGTSGLNLNEEYRNEPVYELEYDGPGSCISHVSYKDQPIIYLNSRHSDSGFSEQYNIS
AEDNQSVISLDAAEEHNDGIIDKMLVKPKRIKATNDANFLNTKSKRVRRYKYKYRNSC
LAPSTKAIKVGKRSAHKSHLAANNPVSFVSKKNHVIDDYFFEELESQSLEQDDSSSLK
PQKKRRKKKAPIYSSFSADLESRRKPVFNTVVEVPTNRYAFTKPNVRNRDSINHDMEF
EEEDSNQELGPIMVVLDSILLKKPFEPPNFFKIQLSDKSFLLSKLNPADIATSLQKIF
RVIIDKGITDTELVHFNESLIAFLVHLDMPELFDLIGEFHREFRSKVNSLRKKAKPIH
FFQIAACQLMFLEISRYNKISAAAKFDMDVKLLDHIVSFFKLLSVCYDSVMKNPMQYL
YTSYYILSAVVDVIHKKEALWDLFQKHPFSPHISLLLVNIFPTKVCRWQVLRLDSEFQ
PLSSAFRFINYCIETCNWNVTNSLILSLDRIFKRRRFSDFEEESDLSQNNKIIYPPTN
QLTSRLMFNRYLHLLTLCELSSSDTQRVIPMGDISMNDSLSVLKNRLNLLIVLATRFD
LNLEKRFQELTRPLYSKEYLNLHTQNTVRTITTLIMQASLSFLEISRIKNHPFSGKFI
ASLFDKLVLQQPSISGVTENFLKEFTNLVSKMKRKSVSMLKFLYPSLVAMSQENIFES
SFFLLLQVYLKSLDVLGPTWVQNYLFQFIKSKAQENERWIECYCQIGKFLVDSGIFTW
WTFFTYNGLDAALHFQLAFHSLIIDFCDTDSFELLKKPLYSIASDLLLISKDDAFYHF
LSNLLKRAHIIVADLKPVSDENELLRLAYIFSKALKKNAYQDLLAVFLSLAKKYYDEG
DISRNFLAKYLEFLNKNCLTELRNNQLFISLRRELGISSDEDEKCAFWDSFNEAGDIL
SKAAFVETGIVQACCTGNEIDGYLDNLSTLFTSTMLESPFAFFSDLVIAHIFENRPFF
DVNIKNFFLSHFIDLFNKVLKMKFEQVSPDEFAELCKVYRALCIECATDDTFNSNSDL
IAAKDAFLVSVLRIADGFWEHDKLLQLRMLDSNMNIPNQIPHTTLQSSLSAIVIKIIE
SNIGKIEASEPFKTFKNT"
gene complement(727605..728885)
/gene="Sfh1"
/locus_tag="R008_L12386"
mRNA complement(727605..728885)
/gene="Sfh1"
/locus_tag="R008_L12386"
/product="Sfh1p"
CDS complement(727605..728885)
/gene="Sfh1"
/locus_tag="R008_L12386"
/note="Similar to YLR321C 'Component of the RSC chromatin
remodeling complex; essential gene required for cell cycle
progression and maintenance of proper ploidy;
phosphorylated in the G1 phase of the cell cycle; Snf5p
paralog'"
/codon_start=1
/product="Sfh1p"
/protein_id="EWG84534.1"
/translation="MSHQNQLIPQAYISNFHNRLTNEDDGIPIFTMAQQTRQHKRAKV
VNYAEYDNDLFDEFNMNGSNFNNADTHYKDNAVSHENTPALTNGVTMDGSEYNVLENM
NGADSIISNNKYDAGSNMVVESLSGLNSNNNASNGPSNKAQAQDIGNAVLPDLQDQHH
NPFNILRYPKIRDTFINGKVVSPYRLNTDQETKANANSGEAIMIPITLDIEHMGHTIK
DQFLWNYNDDSVSPEEFASIYCKDLDMTSATLQTQIANIIKEQLKDLENIAATEIMSD
LHVIINLTCNLQDRFFEDNFQWNLNDKSLTPERFATSIVQDLGLTREFIPLISQSLHE
TILKIKKDWVDGHLIQDHVPNDAAFGYLSGIRLDIDELGSNWCPRVEILTKEEIQKRE
IEKERNLRRLKRETDRLSRRGRRRLDDLETTMRM"
gene complement(729194..729973)
/gene="Cwc24"
/locus_tag="R008_L12396"
mRNA complement(729194..729973)
/gene="Cwc24"
/locus_tag="R008_L12396"
/product="Cwc24p"
CDS complement(729194..729973)
/gene="Cwc24"
/locus_tag="R008_L12396"
/note="Similar to YLR323C 'Essential protein, component of
a complex containing Cef1p; has similarity to S. pombe
Cwf24p'"
/codon_start=1
/product="Cwc24p"
/protein_id="EWG84535.1"
/translation="MFRKRLVNKSSSDEKNQKKRQKINFSEEKLVASDEEKGSSDLMS
LAKSGNSRTLQLSHENEGKLQKKGEDLDKYTLTVNDDSTKEDLLNFERKELAEKAKKR
RPSDDNELVLNMSGKNKRLTKQINQPTNIRTTVLMDFQPDVCKDYKQTGYCGYGDSCK
FLHSRDDFKTGWKLNQEWNADKEDSKAVTLDLEKIPFKCTLCKEDYKSPVVTNCGHYF
CGSCFAKDMKKGTKCFICHKETHGSAKVASDLQKMLNKRKS"
gene 730236..731807
/gene="Pex30"
/locus_tag="R008_L12401"
mRNA 730236..731807
/gene="Pex30"
/locus_tag="R008_L12401"
/product="Pex30p"
CDS 730236..731807
/gene="Pex30"
/locus_tag="R008_L12401"
/note="Similar to YLR324W 'Peroxisomal integral membrane
protein, involved in negative regulation of peroxisome
number; partially functionally redundant with Pex31p;
genetic interactions suggest action at a step downstream
of steps mediated by Pex28p and Pex29p'"
/codon_start=1
/product="Pex30p"
/protein_id="EWG84536.1"
/translation="MSDNTTNVHETRAKFAETLQPRIGGNTTKVIRAALEKNEAESGV
SEDNDNGSLEKVNVATSPLLTSTPPTISKALVKLYPYLILIDEFLNVVTWTGKNIWSS
VLMLCLFITTVEYFETLVKYFGHLAIIAILWGYSLLDNYIEGTLSSSPTLEDIALLMN
RVSLKSDILLSPMVNLGTQDIQRLLYTTVILSPIYVMITWLLLPPRSLMLMVGMFLLT
YHSPWSKVARRLLWKFKIVRLLVFYVTGLDLGGINKDQGIFATVQKQVKKLASTENSN
GVLSDSKPIRFTYVLYENQRRWLGIGWKPSMLSYERTPWTDEFLNEAPSPENFHLPEE
TNTMVWRWVDKTWRLDMTNDGAIQVPNSKARTSADPSPDEGFIYYDNTWKKPSKEDSF
SKYTRRRRWVRTAELVKTSDFDESVINSNRNSAIEQKVEENSTNGLTAEQELGGNKQE
KDNAKKVGEPTTEETKEFAEASNINEGEFERISSTDEEVLKSRARDRLAKVLDDSEEK
EQSNPTIGRDSKKAV"
gene complement(732164..732400)
/gene="Rpl38"
/locus_tag="R008_L12406"
mRNA complement(732164..732400)
/gene="Rpl38"
/locus_tag="R008_L12406"
/product="Rpl38p"
CDS complement(732164..732400)
/gene="Rpl38"
/locus_tag="R008_L12406"
/note="Similar to YLR325C Protein component of the large
(60S) ribosomal subunit, has similarity to rat L38
ribosomal protein"
/codon_start=1
/product="Rpl38p"
/protein_id="EWG84537.1"
/translation="MAREITDIKQFLELTRRADVKTATVKINKKLNKAGKPFRQTKFK
VRGSSSLYTLVINDAGKAKKLIQSLPPTLKVNRL"
gene 733195..733917
/locus_tag="R008_L12416"
mRNA 733195..733917
/locus_tag="R008_L12416"
/product="hypothetical protein"
CDS 733195..733917
/locus_tag="R008_L12416"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84538.1"
/translation="MSGFIKSTLLSLGQDYLEDQYQEFAEQHFQPTRDPFYETNKDGK
KHRRRLPYYCTKDESKAWKKVQNKAWLHDKSLCGCCCWTNTIGWAPLLALLPVIGPLL
MYWVHDKLIELADDRYKLPAEIKVKMHGNIVIDLLISLVPILGSVFAWLHACSTRNAA
IVYNFVGKRALERKQAELMHQKEENEKHSNANTAPPVVGGNKNVNGNRNNSKMYNRPP
VTAPPAPAYTRSTNGRPQRGYR"
gene complement(734148..734408)
/gene="Tma10"
/locus_tag="R008_L12426"
mRNA complement(734148..734408)
/gene="Tma10"
/locus_tag="R008_L12426"
/product="Tma10p"
CDS complement(734148..734408)
/gene="Tma10"
/locus_tag="R008_L12426"
/note="Similar to YLR327C 'Protein of unknown function
that associates with ribosomes; putative homolog of the
F1F0-ATPase synthase regulator Stf2p'"
/codon_start=1
/product="Tma10p"
/protein_id="EWG84539.1"
/translation="MTRTSKWTVHEAKSKPKYFTHNGNFGESPNHVKRGGYGKGNWGK
PGDEINDLIDSGEIKTVFNKTRRGSNSQNNERRLSDLQQYHI"
gene 735375..735475
/locus_tag="R008_L12431"
tRNA 735375..735475
/locus_tag="R008_L12431"
/product="tRNA-Ser"
/note="TS(GCU)L"
assembly_gap 735900..735927
/estimated_length=28
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 735934..737139
/gene="Nma1"
/locus_tag="R008_L12436"
mRNA 735934..737139
/gene="Nma1"
/locus_tag="R008_L12436"
/product="Nma1p"
CDS 735934..737139
/gene="Nma1"
/locus_tag="R008_L12436"
/note="Similar to YLR328W Nicotinic acid mononucleotide
adenylyltransferase, involved in pathways of NAD
biosynthesis, including the de novo, NAD(+) salvage, and
nicotinamide riboside salvage pathways"
/codon_start=1
/product="Nma1p"
/protein_id="EWG84540.1"
/translation="MDPTRAPDFKPPSADEELIPPPDPESKIPKSIPIIPYVLADANS
SIDAPFNIKRKKKHPKHHHHHHHSRKEGNDKKHQHIPLNQDDFQPLSAEVSSEDDDAD
FRSKERYGSDSTTESETRGVQKYQIADLEEVPHGIVRQARILEDYEFPSHRLSKKLLD
PNKLPLVIVACGSFSPITYLHLRMFEMALDAISEQTRFEVIGGYYSPVSDNYQKQGLA
PSYHRVRMCELACERTSSWLMVDAWESLQPSYTRTAKVLDHFNHEINIKRGGVATVTG
EKIGVKIMLLAGGDLIESMGEPNVWADADLHHILGNYGCLIVERTGSDVRSFLLSHDI
MYEHRRNILIIKQLIYNDISSTKVRLFIRRAMSVQYLLPNSVIRYIQEHRLYVDQTEP
VKQVLGNKE"
gene 737463..738354
/gene="Rec102"
/locus_tag="R008_L12441"
mRNA join(737463..737636,737734..738354)
/gene="Rec102"
/locus_tag="R008_L12441"
/product="Rec102p"
CDS join(737463..737636,737734..738354)
/gene="Rec102"
/locus_tag="R008_L12441"
/note="Similar to YLR329W 'Protein involved in early
stages of meiotic recombination; required for chromosome
synapsis; forms a complex with Rec104p and Spo11p
necessary during the initiation of recombination'"
/codon_start=1
/product="Rec102p"
/protein_id="EWG84541.1"
/translation="MARDITFLTVFLESCGAVNNDEAGKLLSAWTSTVRIEGPEPTDS
NSLYIPLLPPGMLKIKLNFKMNDRLVTEEQELFTKLREIVGSSIRFWEEQLFYQVQDV
STIENHVILSLKCTILTDAQISTFISKPRELHTHAKGYPEIYYLSELSTTVNFFSKEG
NYVEISHVIPHFNEYFSSLIVSQLEFEYPMVFSMISRLRLKWQQSSLAPISYALTSNS
VLLPIMLNMIAQDKSSTTAYQILCRRRGPPIQNFQIFSIPAVTYNK"
gene 738684..740615
/gene="Chs5"
/locus_tag="R008_L12446"
mRNA 738684..740615
/gene="Chs5"
/locus_tag="R008_L12446"
/product="Chs5p"
CDS 738684..740615
/gene="Chs5"
/locus_tag="R008_L12446"
/note="Similar to YLR330W Component of the exomer complex,
which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is
involved in export of selected proteins, such as chitin
synthase Chs3p, from the Golgi to the plasma membrane"
/codon_start=1
/product="Chs5p"
/protein_id="EWG84542.1"
/translation="MSSVDVLLTVGKLDASLALLTTQDHHVIEFPTVLLPENVKAGSI
IKMQVSQNLEEEKKQRNHFKSIQAKILEKYGTHKPESPVLKIVNVTQTSCVLAWDPLK
LGSAKLKSLILYRKGIRSMVIPNPFKVTTTKISGLSVDTPYEFQLKLITTSGTLWSEK
VILRTHKMTDMSGITVCLGPLDPLKEISDLQISQCLSHIGARPLQRHVAIDTTHFVCN
DLDNEESNEELIRAKHNNIPIVRPEWVRACEVEKRIVGVRGFYLDADQSILKSYTFPP
VNEEELSYSKENEPVAEVADENKMPEDTTDVEQVASHNDNEGNPSEAKEQGEKSGHEA
APVSPAEDPLHASTALENETTIETVNPSVRSLKSEPVGTPNIEENKADSSAEAVVEEP
NEAVAESSPNEGATGQKSEDTDTHSNEQADNGFVQTEEVAENNIITESARENNEPADD
AAMEFGRPEAEVETPEVNESIEDANEPVEDANEPVEDANEPVEDTSEPVEDTSEPAKN
AGEPVQETNEFTTDIASPRHQEEDIELEAEPKDATESVAVEPSNEDVKPEEKGSEAED
DINNVSKEAASGESTTHQKTEASASLESSAVTEEQETTEAEVNTDDVLSTKEAKKNSG
NSNSNKKKNKKNKKKGKKK"
gene 741612..742742
/gene="Mid2"
/locus_tag="R008_L12461"
mRNA 741612..742742
/gene="Mid2"
/locus_tag="R008_L12461"
/product="Mid2p"
CDS 741612..742742
/gene="Mid2"
/locus_tag="R008_L12461"
/note="Similar to YLR332W 'O-glycosylated plasma membrane
protein that acts as a sensor for cell wall integrity
signaling and activates the pathway; interacts with Rom2p,
a guanine nucleotide exchange factor for Rho1p, and with
cell integrity pathway protein Zeo1p'"
/codon_start=1
/product="Mid2p"
/protein_id="EWG84543.1"
/translation="MLSFTTKNSFRLLLLILSCISTIRAQFFVQSSSSNSSAVSTARS
SVSRVSSSSSILSSSMVSSSSADSSSLTSSTSSRSLVSHTSSSTSIASISFTSFSFSS
DSSTSSSSSASSDSSSSSSFSISSTSATSESSTSSTQTSTSSSSSLSSTPSSSSSPST
ITSAPSTSSTPSTTAYNQGSTITSIINGKTILSNHYTTVTYTPSATADSSNKSKSSGL
SKKNRNIVIGCVVGIGVPLILVILALIYMFCIQSSRTDFIDSDGKVVTAYRANKFTKW
WYMLLGKKVSDEYHSDSPLGGSASSAGGLDLDEADDVMEQSSLFDVRIRDSDSVLPNA
NTADHNNTNSGGEPINSSVASNDIIEEKFYDEQGNELSPRNY"
gene 744852..744923
/locus_tag="R008_L12466"
tRNA 744852..744923
/locus_tag="R008_L12466"
/product="tRNA-Asp"
/note="TD(GUC)L2"
gene complement(745423..745512)
/locus_tag="R008_L12471"
ncRNA complement(745423..745512)
/ncRNA_class="snoRNA"
/locus_tag="R008_L12471"
/product="SNR61"
gene complement(745634..745731)
/locus_tag="R008_L12476"
ncRNA complement(745634..745731)
/ncRNA_class="snoRNA"
/locus_tag="R008_L12476"
/product="SNR55"
gene complement(745874..745961)
/locus_tag="R008_L12481"
ncRNA complement(745874..745961)
/ncRNA_class="snoRNA"
/locus_tag="R008_L12481"
/product="SNR57"
gene complement(746530..746856)
/gene="Rps25b"
/locus_tag="R008_L12486"
mRNA complement(746530..746856)
/gene="Rps25b"
/locus_tag="R008_L12486"
/product="Rps25bp"
CDS complement(746530..746856)
/gene="Rps25b"
/locus_tag="R008_L12486"
/note="Similar to YLR333C 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps25Ap and
has similarity to rat S25 ribosomal protein'"
/codon_start=1
/product="Rps25bp"
/protein_id="EWG84544.1"
/translation="MPPKQQLSKAAKAAAALAGGKKSKKKWSKKSMKDRAQHAVILDQ
EKYDRILKEVPTYRYVSVSVLVDRLKIGGSLARIALRHLEKEGIIKPISKHSKQAIYT
RAAASE"
gene 748135..748206
/locus_tag="R008_L12496"
tRNA 748135..748206
/locus_tag="R008_L12496"
/product="tRNA-Glu"
/note="TE(UUC)L"
gene 748386..750548
/gene="Nup2"
/locus_tag="R008_L12501"
mRNA 748386..750548
/gene="Nup2"
/locus_tag="R008_L12501"
/product="Nup2p"
CDS 748386..750548
/gene="Nup2"
/locus_tag="R008_L12501"
/note="Similar to YLR335W 'Nucleoporin involved in
nucleocytoplasmic transport, binds to either the
nucleoplasmic or cytoplasmic faces of the nuclear pore
complex depending on Ran-GTP levels; also has a role in
chromatin organization'"
/codon_start=1
/product="Nup2p"
/protein_id="EWG84545.1"
/translation="MAKRVADAQIQRETYDSNESDDDVTPSTKVASSAVMNRRKIAMP
KRRMAFKPFGSAKSDETKQASSFSFLNRADGTGEAQVDNSPTTESNSRLKALNLQFKA
KVDDLVLSKPLADLRPLFTRYELYIKNILEAPVKSIENPTQTKGNDAKPAKVEDVQKS
SDSSSEDEVKVEGPKFTIDAKPPISDSVFSFGPKKENRKKDESDSENDIEIKGPEFKF
SGTVSSDVFKLNLSTDKNEKKTETNAKPFSFSSATSTTEQTKSKNPLSLTEATKTNVD
NNSKAEASFTFGTKHAADSQNNKPSFVFGQAAAKPSLEKSSFTFGSTTIEKKNDENST
SNSKPEKSSDSNDSKPSFSFSIPSKNTPDASKPSFNFGVPNSSKNETSKPVFSFGAAT
PSAKEASQEDDNNNVEKPSSKPAFNFISNAGTEKEKESKKDSKPAFSFGISNGSESKD
SDKPSLPSAVDGENDKKEATKPAFSFGINTNTTKTADTKAPTFTFGSSALADNKEDVK
KPFSFGTSQPNNTPSFSFGKTTANLPANSSTSPAPSIPSTGFKFSLPFEQKSGQTTTN
DSKEESTTEATGNESQDATKVDATPEESKPINLQNGEEDEVALFSQKAKLMTFNAETK
SYDSRGVGEMKLLKKKDDPSKVRLLCRSDGMGNVLLNATVVDSFKYEPLAPGNDNLIK
APTVAADGKLVTYIVKFKQKEEGRSFTKAIEDAKKEMK"
gene complement(750653..753352)
/gene="Sgd1"
/locus_tag="R008_L12506"
mRNA complement(750653..753352)
/gene="Sgd1"
/locus_tag="R008_L12506"
/product="Sgd1p"
CDS complement(750653..753352)
/gene="Sgd1"
/locus_tag="R008_L12506"
/note="Similar to YLR336C 'Essential nuclear protein,
required for biogenesis of the small ribosomal subunit;
has a possible role in the osmoregulatory glycerol
response; putative homolog of human NOM1 which is
implicated in acute myeloid leukemia'"
/codon_start=1
/product="Sgd1p"
/protein_id="EWG84546.1"
/translation="MQKTDGIRIPGVILDELKTLDYSQDERFSISEGKKRKRGNGKHL
SRKEKRKMERADKKRKIISTREINSSRLKSAPTSEKRSANAGVKNVGKQANGKNPISS
DESESNENRDSDEVLTDEVAEESGEQAMSAEETMKKLESSKRKAKGIQGAENSGEIKG
NSYEKKHIRNRDTNENFVSYPLAPSDRSAFERDEMDMQYYAKKLGLKGERKAIHAKDE
FDAIGGLLEGLEYFENYGKSDEEYGDFATETNSMRKDDEASEKAFSSDDDLSASDFED
SDGLSESDNDSVADSDDNYRREKENPYVAPTQSVESYVPPSLRKKLDDSENNSTLSEI
SKKVNSSLNKLSDSNITIIITDLNRLYDSLPRQYVTESLTKGILNIIGQNQKLLDGFI
MNYAALAYTLSKLRGIEVGAFFIQKTVEAFLHHYEEEIENISKDQQSKISSKICINIA
TLLSYCYNFGFVSCRLIYDIIRIFVADPNEFTTELLLRIISISGQLIRGDDPSALRDI
RSELLKNAKNLKEQSPRLRFLMDTMSDLKNNRLKPSILATDHHPLKKNLQSILNSSSS
WEPLQVSLDDIKNIDSKGKWWLVGASWRGNMENAFEVSINNENDASKSKKSKISIEDD
LLDDIPDWNIIARQQRMNTDIRRAIFISIMSAQDYLDAFSKLEKLSLKNKQVLEIPRI
VLHCLLADSGSNGYNHYYALVANKICERYSYLSKSFQFLFWDVIKKFEDKEFDSESDT
DEEDDLDDKEKLLRISNQGRFFGSLLANDILKLDVFKHVPFMGGLNTEGMLFMEILLF
QLFLTVAKKSEKKLKMDENGNKRIIYSDDYLRDVLTKNVKSENMLFILKGLKWFINKK
FRYHNFLAGKKGDKAFDRDERRLAWASKAAKSIIDKELENIDS"
gene complement(753606..756059)
/gene="Vrp1"
/locus_tag="R008_L12511"
mRNA complement(753606..756059)
/gene="Vrp1"
/locus_tag="R008_L12511"
/product="Vrp1p"
CDS complement(753606..756059)
/gene="Vrp1"
/locus_tag="R008_L12511"
/note="Similar to YLR337C 'Proline-rich actin-associated
protein involved in cytoskeletal organization and
cytokinesis; related to mammalian Wiskott-Aldrich syndrome
protein (WASP)-interacting protein (WIP)'"
/codon_start=1
/product="Vrp1p"
/protein_id="EWG84547.1"
/translation="MAGAPAPPPPPPPPALGGSAPKPAKSVMQGRDALLGDIRKGMKL
KKAETNDRSAPIVGGGVVSSASGSSGTVSSKGPSMSAPPIPGMGAPQLGDILAGGIPK
LKHINNNASTKPSPSASAPPIPGAVPSVAAPPIPNAPLSSAPAVPSIPSSSAPPIPDI
PSSAAPPIPIVPSSPAPPLPLSGASAPKVPQNRPHMPSVRPAHRSHQRKSSNISLPSV
SAPPLPSASLPTHVSNPPQAPPPPPTPTFGLDSKNIKPTDNAVSPPSSEVPAGGLPFL
AEINARRSERGAVEGVSSTKIQTENHKSPSQPPLPSSAPPIPTSHAPPLPPTAPPPPS
LPNVTSAPKKATSAPAPPPPPLPAAMSSASTNSVKATPVPPTLAPPLPNTTSVPPNKA
SSMPAPPPPPPPPPGAFSTSSALSASSIPLAPPPPPPPPSVATSVPSAPPPPLTLTTN
KPSASSNQSKISSSSSSSAVTPGGPLPFLAEIQKKRDDRFVVGGDTGYTTQDKQEDVI
GSSKDDNVRPSPVSPSINPPKQSSQNGMSFLDEIESKLHKQTSSNAFNAPPPHTDAMA
PPLPPSAPPPPITSLPTPTASGDDHTNDKSETVLGMKKAKAPALPGHVPPPPVPPVLS
DDSKNNAPAASLLHDVLPSSNLEKPPSPPVAAAPPLPTFSAPSLPQQSVSTSIPSPPP
VAPTLSVRTETESISKNPTKSPPPPPSPSTMDTGTSNSPSKNLKQRLFSTGGSTLQHK
HNTHTNQPDVDVGRYTIGRSNSIVGAKSGNERIVIDDSRFKWTNVSQMPKPRPFQNKT
KLYPSGKGSSVPLDLTLFT"
gene 756839..757777
/gene="Rpp0"
/locus_tag="R008_L12526"
mRNA 756839..757777
/gene="Rpp0"
/locus_tag="R008_L12526"
/product="Rpp0p"
CDS 756839..757777
/gene="Rpp0"
/locus_tag="R008_L12526"
/note="Similar to YLR340W 'Conserved ribosomal protein P0
of the ribosomal stalk, which is involved in interaction
between translational elongation factors and the ribosome;
similar to rat P0, human P0, and E. coli L10e;
phosphorylated on serine 302'"
/codon_start=1
/product="Rpp0p"
/protein_id="EWG84548.1"
/translation="MGGVREKKAEYFAKLREYLEEYKSLFVVGVDNVSSQQMHEVRKE
LRGRAVVLMGKNTMVRRAIRGFLSDLPDFEKLLPFVKGNVGFVFTNEPLTEIKSVIVS
NRVAAPARAGAVAPEDIWVRAVNTGMEPGKTSFFQALGVPTKIARGTIEIVSDVKVVD
AGNKVGQSEASLLNLLNISPFTFGLTVVQVYDNGQVFPSSILDITDEELVSHFVSAVS
TIASISLAIGYPTLPSVGHTLINNYKDLLAVAIAASYHYPEIEDLVDRIENPEKYAAA
APAASSAASDDAAPAEEAAAEEEEESDDDMGFGLFD"
gene 758336..759769
/gene="Spo77"
/locus_tag="R008_L12536"
mRNA 758336..759769
/gene="Spo77"
/locus_tag="R008_L12536"
/product="Spo77p"
CDS 758336..759769
/gene="Spo77"
/locus_tag="R008_L12536"
/note="Similar to YLR341W 'Meiosis-specific protein of
unknown function, required for spore wall formation during
sporulation; dispensable for both nuclear divisions during
meiosis'"
/codon_start=1
/product="Spo77p"
/protein_id="EWG84549.1"
/translation="MFRRGDNSNFNVQNSFFLPLEYEFTVKDNVPSKKKSSVGFFPLD
DSLFTSKNNSGHHKSEQLHRGNAETIRSQFGTDAVPIRIDEKEGKWDRIQDDNSSNLN
YQINNSNDPASSGKYSQSIDRNHIAESKFSKKNGKIDSLRSNSATFVLNTADEDVIEF
SLDDNVPYAELLSGATLEKCSLTLNEINKKLFNTLYDFRVSKDNPEENLVELILPNCV
VLSNFFEDIELLADSSDEAFEKSTFINTIEFIVHDIWVETLIKNINLLQMLDADLKCY
NDKYIICKLKGQYPSTNIVDIMSRLKHFSNSILETFKFGIELKEQDQCHNRNTIINYV
LFSRVFSTIVLEIQKCFILIVKFMYSVDFLEKFSDEVFLSFIEILIKIVFEHQIPQLF
LGIDEIIQLWLKNNEVKRQQLLSAWCNGTVQDMKQSQQRGSSNAESESIASSTEEDEE
GLQFNKWDVIEPFIDNIKALNQSKSHM"
assembly_gap 760776..760858
/estimated_length=83
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 760955..766585
/gene="Fks1"
/locus_tag="R008_L12541"
mRNA 760955..766585
/gene="Fks1"
/locus_tag="R008_L12541"
/product="Fks1p"
CDS 760955..766585
/gene="Fks1"
/locus_tag="R008_L12541"
/note="Similar to YLR342W 'Catalytic subunit of
1,3-beta-D-glucan synthase, functionally redundant with
alternate catalytic subunit Gsc2p; binds to regulatory
subunit Rho1p; involved in cell wall synthesis and
maintenance; localizes to sites of cell wall remodeling'"
/codon_start=1
/product="Fks1p"
/protein_id="EWG84550.1"
/translation="MNTDQQPYQGQTDYTQGPDNGQSQEQDYDQYGQPLYPSQADGYY
DPNVAAGTEADMYGQQPPNESYDQDYTNGEYYGQPPNMAAQDGENFSDFSSYGPPGTP
GYDSYGGQYTPSQMSYGEPNSSGTSTPIYGNYDPNAIAMALPNEPYPAWTADSQSPVS
IEQIEDIFIDLTNRLGFQRDSMRNMFDHFMVLLDSRSSRMSPDQALLSLHADYIGGDT
ANYKKWYFAAQLDMDDEVGFRNMSLGKLSRKARKAKKKNKKAMEEANPEDTEETLNKI
EGDNSLEAADFRWKAKMNQLSPLERVRHIALYLLCWGEANQVRFTAECLCFIYKCALD
YLDSPLCQQRQEPMPEGDFLNRVITPIYHFIRNQVYEIIDGRFVKRERDHNKIVGYDD
LNQLFWYPEGIAKIVLEDGTKLIELPLEERYLRLGDVVWDDVFFKTYKETRTWLHLVT
NFNRIWVMHISIFWMYFAYNSPTFYTHNYQQLVDNQPLAAYRWASCALGGTVASLIQI
VATLCEWSFVPRKWAGAQHLSRRFWFLCIIFGINLGPIIFVFAYDKDTVYSTAAHVVA
AVMFFVAVATIIFFSIMPLGGLFTSYMKKSTRRYIASQTFTAAFAPLHGLDRWMSYLV
WVTVFAAKYSESYYFLVLSLRDPIRILSTTAMRCTGEYWWGAVLCKVQPKIVLGLVIA
TDFILFFLDTYLWYIIVNTIFSVGKSFYLGISILTPWRNIFTRLPKRIYSKILATTDM
EIKYKPKVLISQVWNAIIISMYREHLLAIDHVQKLLYHQVPSEIEGKRTLRAPTFFVS
QDDNNFETEFFPRDSEAERRISFFAQSLSTPIPEPLPVDNMPTFTVLTPHYAERILLS
LREIIREDDQFSRVTLLEYLKQLHPVEWECFVKDTKILAEETAAYEGNENEAEKEDAL
KSQIDDLPFYCIGFKSAAPEYTLRTRIWASLRSQTLYRTISGFMNYSRAIKLLYRVEN
PEIVQMFGGNAEGLERELEKMARRKFKFLVSMQRLAKFKPHELENAEFLLRAYPDLQI
AYLDEEPPLTEGEEPRIYSALIDGHCEILDNGRRRPKFRVQLSGNPILGDGKSDNQNH
ALIFYRGEYIQLIDANQDNYLEECLKIRSVLAEFEELNVEQVNPYAPGLRYEEQTTNH
PVAIVGAREYIFSENSGVLGDVAAGKEQTFGTLFARTLSQIGGKLHYGHPDFINATFM
TTRGGVSKAQKGLHLNEDIYAGMNAMLRGGRIKHCEYYQCGKGRDLGFGTILNFTTKI
GAGMGEQMLSREYYYLGTQLPVDRFLTFYYAHPGFHLNNLFIQLSLQMFMLTLVNLSS
LAHESIMCIYDRNKPKTDVLFPIGCYNFQPAVDWVRRYTLSIFIVFWIAFVPIVVQEL
IERGLWKATQRFFCHLLSLSPMFEVFAGQIYSSALLSDLAIGGARYISTGRGFATSRI
PFSILYSRFAGSAIYMGARSILMLLFGTVAHWQAPLLWFWASLSSLIFAPFVFNPHQF
AWEDFFLDYRDYIRWLSRGNNQYHRNSWIGYVRMSRARITGFKRKLVGDESEKAAGDA
SRAHRTNLIMAEIIPCAIYAAGCFIAFTFINAQTGVKTTDDDRVNSVLRIIICTLAPI
AVNLGVLFFCMGMSCCSGPLFGMCCKKTGSVMAGIAHGVAVIVHIAFFIVMWVLESFN
FVRMLIGVVTCIQCQRLIFHCMTALMLTREFKNDHANTAFWTGKWYGKGMGYMAWTQP
SRELTAKVIELSEFAADFVLGHVILICQLPLIIIPKIDKFHSIMLFWLKPSRQIRPPI
YSLKQSRLRKRMVKKYCSLYFLVLAIFAGCIIGPAVASAKIHDQIGDSLTGVVHNLFQ
PVNTTNNDTGFQMSTYQSHYYTHTPSLKTWSTIK"
gene 766767..766942
/locus_tag="R008_L12546"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 767053..768720
/gene="Gas2"
/locus_tag="R008_L12551"
mRNA 767053..768720
/gene="Gas2"
/locus_tag="R008_L12551"
/product="Gas2p"
CDS 767053..768720
/gene="Gas2"
/locus_tag="R008_L12551"
/note="Similar to YLR343W '1,3-beta-glucanosyltransferase,
involved with Gas4p in spore wall assembly; has similarity
to Gas1p'"
/codon_start=1
/product="Gas2p"
/protein_id="EWG84551.1"
/translation="MNKKQNFYAAIIVAIFLCLQLSHGSSGVSFEKTPAIKIVGNKFF
DSENGEQFFIKGIAYQLQRSEEELSNANGAFERSYIDALADPKICLRDIPFLKMLGVN
TLRVYAIDPTKSHDICMEALSAEGMYVLLDLSEPDISINRENPSWDVHIFERYKSVID
AMSSFPNLLGYFAGNEVTNDHTNTFASPFVKAAIRDAKEYISHSNHRKIPVGYSTNDD
AMTRDNLARYFVCGDVKADFYGINMYEWCGYSTYGTSGYRERTKEFEGYPIPVFFSEF
GCNLVRPRPFTEVSALYGKKMSSVWSGGLAYMYFEEENEYGVVKINDNDGVDILPDFK
NLKKEFAKADPKGITEEEYLTAKEPTEVEGIECPHIAVGVWEANEKLPETPDRSKCAC
LDEVLPCEIVPFGAESGKYEEYFSYMCSKVDCSDIHANGKTGEYGEFSDCSVEQKLSL
QLSKLYYKIGANDRHCPLNDKNVYFNLQSLQPLTSESICKNVFDSIRNITYNHGDYSK
SNPSRSKESLNVKYPSSEERENHGTIAFKTSGFVILLISMIAAGILL"
gene 769908..769979
/locus_tag="R008_L12556"
tRNA 769908..769979
/locus_tag="R008_L12556"
/product="tRNA-Arg"
/note="tRNA-Arg (Arg4);
TR(CCG)L"
gene 770608..771438
/gene="Rpl26a"
/locus_tag="R008_L12561"
mRNA join(770608..770626,771074..771438)
/gene="Rpl26a"
/locus_tag="R008_L12561"
/product="Rpl26ap"
CDS join(770608..770626,771074..771438)
/gene="Rpl26a"
/locus_tag="R008_L12561"
/note="Similar to YLR344W 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl26Bp and
has similarity to E. coli L24 and rat L26 ribosomal
proteins; binds to 5.8S rRNA'"
/codon_start=1
/product="Rpl26ap"
/protein_id="EWG84552.1"
/translation="MAKQSLDVSSDRRKARKAYFTAPSSERRVLLSAPLSKELRAQYG
IKALPIRRDDEVLVVRGSKKGQEGKISSVYRLKFAVQVDKVTKEKVNGASVPINLHPS
KLVITKLHLDKDRKALIQRKGGKLE"
gene 771806..773335
/locus_tag="R008_L12566"
mRNA 771806..773335
/locus_tag="R008_L12566"
/product="hypothetical protein"
CDS 771806..773335
/locus_tag="R008_L12566"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84553.1"
/translation="MPNVLSDDEELLNGLGSEIMKPSRQGNHMARTVKRWVNKERATT
ADLKNVNIDGVHGPVNTESYISPGQLYSTDSGNLFHAGRILVVLVGLPATSKTLLSVA
ITRYTRWLGVRTKSFHFSEYKESARNIPSDYFCVVPTSKEGVAFVEKLRMQMLNDILA
FFNDLSGQLAIYDALNIRKIDRKNLETTFSEIGVKVLFIESIVSDQEIMNRNIALALE
SNDYKGLSTDEAIDEYMRRLSVNEPYYEMMTHDEELSYIKYINLGKQIIVKDNIHGYL
VNKIVFFLMNLRQKKGCVYFARCGTSDKDNYIHDEELNEEGIHYSQVLKDFVLQRIKQ
KRQAKKNSDSLVEVIDGSHDEDLKTSLIVWTGPRKRTHDTALFFSKEGIKVQQRSELR
QLNPGSIADLTDQQIMDKFPSEYKESLKDPYHFRFPRAESYHDLAVRMEPLLLEMEHT
SKDILIIAHESTLRVLYGYLMACTCVELPNLNFTRDKLVEISFSPFCNTVELLNIPLT
S"
gene complement(773581..773886)
/locus_tag="R008_L12571"
mRNA complement(773581..773886)
/locus_tag="R008_L12571"
/product="hypothetical protein"
CDS complement(773581..773886)
/locus_tag="R008_L12571"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84554.1"
/translation="MQSISNCPIGLVSKNTINSASTIAEWVACPWKYINVVGSGRYVS
NKPDKITRYDLLKAAQEAEMQELLTRNNMKGRHKRNKKSKIALETIAEENSSSESLF"
gene complement(775064..777649)
/gene="Kap95"
/locus_tag="R008_L12581"
mRNA complement(775064..777649)
/gene="Kap95"
/locus_tag="R008_L12581"
/product="Kap95p"
CDS complement(775064..777649)
/gene="Kap95"
/locus_tag="R008_L12581"
/note="Similar to YLR347C 'Karyopherin beta, forms a
complex with Srp1p/Kap60p; interacts with nucleoporins to
mediate nuclear import of NLS-containing cargo proteins
via the nuclear pore complex; regulates PC biosynthesis;
GDP-to-GTP exchange factor for Gsp1p'"
/codon_start=1
/product="Kap95p"
/protein_id="EWG84555.1"
/translation="MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLS
SQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQFAQRWITQVSPEAKNQIKTNALT
ALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALG
YMCESADPQSQALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMER
EGERNYLMQVVCEATQAEDIEVQAAAFGCLCKIMSLYYTFMKPYMEQALYALTIATMK
SPNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALSSIKDVVPNLLNLLT
RQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMA
FGSIMDGPDKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQ
HLPGVVQACLIGLQDHPKVATNCSWTIINLVEQLAEATPSPIYNFYPALVDGLIGAAN
RIDNEFNARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQLTLEDA
QSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISA
LAASLGKGFEKYLETFSPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMM
NVLAQMISNPNARRELKPAVLSVFGDIASNIGADFIPYLNDIMALCVAAQNTKPENGT
LEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDPQLYSEDAT
SRAAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTKSGQLFSQATKDTARWAREQQK
RQLSL"
gene complement(778211..779107)
/gene="Dic1"
/locus_tag="R008_L12586"
mRNA complement(778211..779107)
/gene="Dic1"
/locus_tag="R008_L12586"
/product="Dic1p"
CDS complement(778211..779107)
/gene="Dic1"
/locus_tag="R008_L12586"
/note="Similar to YLR348C Mitochondrial dicarboxylate
carrier, integral membrane protein, catalyzes a
dicarboxylate-phosphate exchange across the inner
mitochondrial membrane, transports cytoplasmic
dicarboxylates into the mitochondrial matrix"
/codon_start=1
/product="Dic1p"
/protein_id="EWG84556.1"
/translation="MSTNAKESAGKNIKYPWWYGGAAGIFATMVTHPLDLAKVRLQAA
PMPKPTLFRMLESILANEGVVGLYSGLSAAVLRQCTYTTVRFGAYDLLKENVIPREQL
TNMAYLLPCSMFSGAIGGLAGNFADVVNIRMQNDSALEAAKRRNYKNAIDGVYKIYRY
EGGLKTLFTGWKPNMVRGILMTASQVVTYDVFKNYLVTKLDFDASKNYTHLTASLLAG
LVATTVCSPADVMKTRIMNGSGDHQPALKILADAVRKEGPSFMFRGWLPSFTRLGPFT
MLIFFAIEQLKKHRVGMPKEDK"
assembly_gap 779268..779316
/estimated_length=49
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 779986..780636
/gene="Orm2"
/locus_tag="R008_L12601"
mRNA 779986..780636
/gene="Orm2"
/locus_tag="R008_L12601"
/product="Orm2p"
CDS 779986..780636
/gene="Orm2"
/locus_tag="R008_L12601"
/note="Similar to YLR350W 'Evolutionarily conserved
protein, similar to Orm1p, required for resistance to
agents that induce unfolded protein response; Orm1p and
Orm2p together control membrane biogenesis by coordinating
lipid homeostasis with protein quality control'"
/codon_start=1
/product="Orm2p"
/protein_id="EWG84557.1"
/translation="MIDRTKNESPAFEESPLTPNVSNLKPFPSQSNKISTPVTDHRRR
RSSSVISHVEQETFEDENDQQMLPNMNATWVDQRGAWLIHIVVIVLLRLFYSLFGSTP
KWTWTLTNMTYIIGFYIMFHLVKGTPFDFNGGAYDNLTMWEQINDETLYTPTRKFLLI
VPIVLFLISNQYYRDDMTLFLSNLAVTVLIGVVPKLGITHRLRISIPGITGRAQIS"
gene complement(780747..781622)
/gene="Nit3"
/locus_tag="R008_L12606"
mRNA complement(780747..781622)
/gene="Nit3"
/locus_tag="R008_L12606"
/product="Nit3p"
CDS complement(780747..781622)
/gene="Nit3"
/locus_tag="R008_L12606"
/note="Similar to YLR351C 'Nit protein, one of two
proteins in S. cerevisiae with similarity to the Nit
domain of NitFhit from fly and worm and to the mouse and
human Nit protein which interacts with the Fhit tumor
suppressor; nitrilase superfamily member'"
/codon_start=1
/product="Nit3p"
/protein_id="EWG84558.1"
/translation="MSASKILSQKIKVALVQLSGSSPDKMANLQRAATFIERAMKEQP
DTKLVVLPECFNSPYSTDQFRKYSEVINPKEPSTSVQFLSNLANKFKIILVGGTIPEL
DPKTDKIYNTSIIFNEDGKLIDKHRKVHLFDVDIPNGISFHESETLSAGEKSTTIDTK
YGKFGVGICYDMRFPELAMLSARKGAFAMIYPSAFNNVTGPLHWHLLARSRAVDNQVY
VMLCSPARNLQSSYHAYGHSIVVDPRGKIVAEAGEGEEIIYAELDPEVIESFRQAVPL
TKQRRFDVYSDVNAH"
gene 782370..784813
/locus_tag="R008_L12611"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
assembly_gap 783631..783644
/estimated_length=14
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 785625..787433
/gene="Bud8"
/locus_tag="R008_L12616"
mRNA 785625..787433
/gene="Bud8"
/locus_tag="R008_L12616"
/product="Bud8p"
CDS 785625..787433
/gene="Bud8"
/locus_tag="R008_L12616"
/note="Similar to YLR353W 'Protein involved in bud-site
selection; diploid mutants display a unipolar budding
pattern instead of the wild-type bipolar pattern, and bud
at the proximal pole'"
/codon_start=1
/product="Bud8p"
/protein_id="EWG84559.1"
/translation="MIQSDEDNLDSSETTASTSYSVTSSVSSRLQLRTSLFFENLNGA
HGNPDAETEMATVAYETTSRGQGFAVYINNERFSQIMGASTSSSSSSNSSSITQFHDT
QDNNIPSNTTVRPTSLRRDNEDTVPLRNVTPSQNAAVRPERAVNSPSSQRLSCALTIS
TSVLMGEDVEGSPIEQEHSRVVSSLYSSLANRGNDESKNGTPPRPTSIEPNETTEHSF
FSYHYDDTLEPDVEEAVRLTKNKTSNVNFISSTGSKGEGETEDEVIDQYEPVNESKFI
PHKLKIPEKAGSIKSSTSDDSHSPGAPGTSARRIKIPQSPSLIGNILIPSHNSDSSNE
SSPKDHIGHNNEEQFNSKSMRKPSTSLEEEGPPIGLPSIPVLRSVSGSSKWTKTPLRL
ESGNSTKSDPFSRYEGHKTPSPLTKMNKKNKTLPEHGQPLVLAPIKSQSSESDTGQNS
IIEKPARSIRRKQQEKTDNRKEDRHDAENIDLEARMPIQHIDTASIHSFDSGQNGFRD
VYSIENIIVILLCCSIVPPLFFIIGCSSRRKLVSDYRLMRLLMNKEHRAALLQGFIWD
VDLRWFRMFCLILGAAETVIVMAGIAIGFGVGITRE"
gene complement(787620..788627)
/gene="Tal1"
/locus_tag="R008_L12621"
mRNA complement(787620..788627)
/gene="Tal1"
/locus_tag="R008_L12621"
/product="Tal1p"
CDS complement(787620..788627)
/gene="Tal1"
/locus_tag="R008_L12621"
/note="Similar to YLR354C 'Transaldolase, enzyme in the
non-oxidative pentose phosphate pathway; converts
sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate
to erythrose 4-phosphate and fructose 6-phosphate'"
/codon_start=1
/product="Tal1p"
/protein_id="EWG84560.1"
/translation="MSEPAQKKQKVANNSLEQLKASGTVVVADTGDFGSIAKFQPQDS
TTNPSLILAAAKQPTYAKLIDVAVEYGKKHGKTTEEQVENAVDRLLVEFGKEILKIVP
GRVSTEVDARLSFDTQATIEKARHIIKLFEQEGVSKERVLIKIASTWEGIQAAKELEE
KDGIHCNLTLLFSFVQAVACAEAQVTLISPFVGRILDWYKSSTGKDYKGEADPGVISV
KKIYNYYKKYGYKTIVMGASFRSTDEIKNLAGVDYLTISPALLDKLMNSTEPFPRVLD
PVSAKKEAGDKISYISDESKFRFDLNEDAMATEKLSEGIRKFSADIVTLFDLIEKKVT
A"
gene complement(789341..790528)
/gene="Ilv5"
/locus_tag="R008_L12626"
mRNA complement(789341..790528)
/gene="Ilv5"
/locus_tag="R008_L12626"
/product="Ilv5p"
CDS complement(789341..790528)
/gene="Ilv5"
/locus_tag="R008_L12626"
/note="Similar to YLR355C 'Bifunctional acetohydroxyacid
reductoisomerase and mtDNA binding protein; involved in
branched-chain amino acid biosynthesis and maintenance of
wild-type mitochondrial DNA; found in mitochondrial
nucleoids'"
/codon_start=1
/product="Ilv5p"
/protein_id="EWG84561.1"
/translation="MLRTQAARLICNSRVITAKRTFALATRAAAYSRPAARFVKPMIT
TRGLKQINFGGTVETVYERADWPREKLLDYFKNDTFALIGYGSQGYGQGLNLRDNGLN
VIIGVRKDGASWKAAIEDGWVPGKNLFTVEDAIKRGSYVMNLLSDAAQSETWPAIKPL
LTKGKTLYFSHGFSPVFKDLTHVEPPKDLDVILVAPKGSGRTVRSLFKEGRGINSSYA
VWNDVTGKAHEKAQALAVAIGSGYVYQTTFEREVNSDLYGERGCLMGGIHGMFLAQYD
VLRENGHSPSEAFNETVEEATQSLYPLIGKYGMDYMYDACSTTARRGALDWYPIFKNA
LKPVFQDLYESTKNGTETKRSLEFNSQPDYREKLEKELDTIRNMEIWKVGKEVRKLRP
ENQ"
gene 791596..792189
/locus_tag="R008_L12631"
mRNA 791596..792189
/locus_tag="R008_L12631"
/product="hypothetical protein"
CDS 791596..792189
/locus_tag="R008_L12631"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84562.1"
/translation="MSVCLAITKGIAVSSIGLYSGLLASASLITSTTPLEVLTGSLTP
TLTTLKNAATALGAFASTFFCVSFFGAPPSLRHPYLLYGMLAAPLSSFVLGCASNYQS
RKYSKVSKESSLFPEDSKPAASELSDSIIDLGEDNHASENTPRDGKPAATTVSKPAEA
LHTGPPIHTKNLIAATAIAIVGFVQAVIGVYGEGQFI"
gene 792606..795275
/gene="Rsc2"
/locus_tag="R008_L12636"
mRNA 792606..795275
/gene="Rsc2"
/locus_tag="R008_L12636"
/product="Rsc2p"
CDS 792606..795275
/gene="Rsc2"
/locus_tag="R008_L12636"
/note="Similar to YLR357W 'Component of the RSC chromatin
remodeling complex; required for expression of mid-late
sporulation-specific genes; involved in telomere
maintenance'"
/codon_start=1
/product="Rsc2p"
/protein_id="EWG84563.1"
/translation="MMPDDNSNSSTQNSSALYKDLRKEYESLFTLKEDSGLEISPIFN
VLPPKKDYPDYYAVIKNPVSFNTLKKRIPHYTDAQQFMNDVVQIPWNAKTYNTRDSGI
YKYALVLEKYIKDTIYPNLKEKYPQLVYPDLGPLPDEPGYEEFQQKLREKAEEVARAN
AARAESSSSMNSTEAARRLRKTRTSVKRESEPGTDTNNDEDYEATDMDIDNPKDADFP
DLIRKPLININPYTRKPLRDNRSTTPSHSGTPQPLGPRHRQVSRTQVKRGRPPIIDLP
YIQRMKNVMKVLKKEVLDSGIGLTDLFERLPDRHRDANYYIMIANPISLQDINKKVKT
RRYKTFQEFQNEFNLMLTNFRISHRGDPESIKISNILEKTFTSLARFELSKPDRSFIP
EGELRYPLDEVIVNNISYHVGDWALLRNQNDPQKPIVGQIFRLWKTPDGKQWLNACWY
YRPEQTVHRVDRLFYKNEVMKTGQYRDHLVSNLVGKCYVIHFTRYQRGNPDMKLEGPL
FVCEFRYNESDKIFNKIRTWKACLPEEIRDLDEGTIPVNGRKFFKYPSPIRHLLPANA
TPHDRVPEPTMGSPDAPPLVGAVYMRPKMQRDDLGEYATSDDCPRYIIRPNDSPEEGQ
VDIETGTITTNTPTANALPKTGYSSSKLSSLRYNRSSMSLENQNAIGQQQIPLSRVGS
PGAGGALTVQGLKQHQLQRLQQQQHQYQQQKRSQASRYNIPTIIDDLTSQASRGNLGN
IMIDAASSFVLPISITKNVDVLQRTDLHSQTKRSGREEMFPWKKTKGEILWFRGPSVI
VNERIINSGDPHLSLPLNRWFTTNKKRKLEYEEVEETMEDVTGKDKDDDGLEPDVENE
KESLPGPFVLGLRPSAKFTAHRLSMLRPPSSSS"
gene 795557..797005
/gene="Ade13"
/locus_tag="R008_L12646"
mRNA 795557..797005
/gene="Ade13"
/locus_tag="R008_L12646"
/product="Ade13p"
CDS 795557..797005
/gene="Ade13"
/locus_tag="R008_L12646"
/note="Similar to YLR359W 'Adenylosuccinate lyase,
catalyzes two steps in the 'de novo' purine nucleotide
biosynthetic pathway; expression is repressed by adenine
and activated by Bas1p and Pho2p; mutations in human
ortholog ADSL cause adenylosuccinase deficiency'"
/codon_start=1
/product="Ade13p"
/protein_id="EWG84564.1"
/translation="MPDYDNYTTPLSSRYASKEMSATFSLRNRFSTWRKLWLNLAIAE
KELGLTVVTDEAIEQMRKHVEITDDEIAKASAQEAIVRHDVMAHVHTFGETCPAAAGI
IHLGATSCFVTDNADLIFIRDAYDIIIPKLVNVINRLAKFAMEYKDLPVLGWTHFQPA
QLTTLGKRATLWIQELLWDLRNFERARNDIGLRGVKGTTGTQASFLALFHGNHDKVEA
LDERVTELLGFDKVYPVTGQTYSRKIDIDVLAPLSSFAATAHKMATDIRLLANLKEVE
EPFEKSQIGSSAMAYKRNPMRCERVCSLARHLGSLFSDAVQTASVQWFERTLDDSAIR
RISLPSAFLTADILLSTLLNISSGLVVYPKVIERRIKGELPFMATENIIMAMVEKNAS
RQEVHERIRVLSHQAAAVVKEEGGENDLIERVKRDEFFKPIWEELDSLLEPSTFVGRA
PQQVEKFVQKDVNNALQPFQKYLNDEQVKLNV"
gene 797399..798718
/gene="Vps38"
/locus_tag="R008_L12651"
mRNA 797399..798718
/gene="Vps38"
/locus_tag="R008_L12651"
/product="Vps38p"
CDS 797399..798718
/gene="Vps38"
/locus_tag="R008_L12651"
/note="Similar to YLR360W 'Part of a Vps34p
phosphatidylinositol 3-kinase complex that functions in
carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p
to promote production of phosphatidylinositol 3-phosphate
(PtdIns3P) which stimulates kinase activity'"
/codon_start=1
/product="Vps38p"
/protein_id="EWG84565.1"
/translation="MKRFLLSRRQRHLRMICFHNISLFRANGDSKLIKEYGDGFIPCF
FILESIRGELLYVSEVQSGSLRKLSFQELPKLTGASTMIVLKLVGLVPSDILCTISSD
KNGIIDDKWCVLCTYTIDLNKLQPINEDTVLITGTNAPVLDLIDGSYTLAAEKIKPLK
GLVSSHKRNISQVKIKFSLAYSSLLKLNKLLEYSSQVHEEINEISSKIEDDFLSLKNQ
NHWYMRTVQKSIETLEKEALQRKKSKKNIEMAQLESNDTINHSKTELSLMSQDESIND
DYGSIYSRFVQIKDRLDQLRFKKLYQLIGIFHSTDLFNSDRGYIYFEKPSSVNDVINR
LKLKPLNIEILLRQAGESTKHREYVNSQLGYYLLFLHLTAIQIFKAPLPYRLMYYGST
SVIDSQYPLYFTDQMISKHQAKLIKAIHYFNADILQFKQILENYRPT"
gene complement(798684..800420)
/gene="Dcr2"
/locus_tag="R008_L12656"
mRNA complement(798684..800420)
/gene="Dcr2"
/locus_tag="R008_L12656"
/product="Dcr2p"
CDS complement(798684..800420)
/gene="Dcr2"
/locus_tag="R008_L12656"
/note="Similar to YLR361C 'Phosphoesterase involved in
downregulation of the unfolded protein response, at least
in part via dephosphorylation of Ire1p; dosage-dependent
positive regulator of the G1/S phase transition through
control of the timing of START'"
/codon_start=1
/product="Dcr2p"
/protein_id="EWG84566.1"
/translation="MIRLPRLYQRYLLYLVVFVVIALFYFLQAPRVEEHIGFDLALPI
SHVDNLWFQNKGLEGFSNDDKLVVNIGYHECFHIGRFYEGCFNRHELKSTLTDGHQYL
QRKRIHKDLRGSFGRRWFGKSEYLYYDVLYPALMDYFGSNLEKLNVEAVTGISKYPKD
KSLPFMDVSITFEPISIELLQKRSYISDINILFGVDCIQPIANWTLQKEFPLVKYRYS
EPAYLTYKFIGTRPVDTGAQRLQETDEGKFKIVQLADLHLGVGESECIDEYPKHEACK
ADPKTETFVQQVLDIEKPQLVVFTGDQIMGDRSIQDSETVLLKAVAPVIARKIPWAMV
WGNHDDEGSLTRWQLSEIASVLPYSLFKFSPHDTHDNTFGVGNYIYQIFSDNDTELPV
GTLYFLDSHKYSTVGKIYPGYDWIKESQWKYIEDYHDVNLKFKTGLSMAFFHIPLPEY
LNIESKTHPGEKNPLIGMYKEGVTAPKYNSEGITTLDRLSVDVVSCGHDHCNDYCLRD
DSTPNKIWLCYGGGGGEGGYAGYGGTERRIRIYEINVNENNIHTWKRLNGSPKEIFDF
QSMLDGNSPESV"
gene complement(800679..800972)
/locus_tag="R008_L12666"
mRNA complement(800679..800972)
/locus_tag="R008_L12666"
/product="hypothetical protein"
CDS complement(800679..800972)
/locus_tag="R008_L12666"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84567.1"
/translation="MAVSNNNNSNSKERTQNIKEVEQKLGENPKITLKGGGKTKIMDF
EQLRKPHCVRPSARFPVEDTAGGLLRTGGHRPQLSDEEVSKRHHEQSHGQEDH"
gene 801159..803312
/gene="Ste11"
/locus_tag="R008_L12671"
mRNA 801159..803312
/gene="Ste11"
/locus_tag="R008_L12671"
/product="Ste11p"
CDS 801159..803312
/gene="Ste11"
/locus_tag="R008_L12671"
/note="Similar to YLR362W 'Signal transducing MEK kinase
involved in pheromone response and pseudohyphal/invasive
growth pathways where it phosphorylates Ste7p, and the
high osmolarity response pathway, via phosphorylation of
Pbs2p; regulated by Ste20p and Ste50p'"
/codon_start=1
/product="Ste11p"
/protein_id="EWG84568.1"
/translation="MEQTQTAEGTDLLIGDEKTNDLPFVQLFLEEIGCTQYLDSFIQC
NLVTEEEIKYLDKDILIALGVNKIGDRLKILRKSKSFQRDKRIEQVNRLKNLMEKVSS
LSTATLSMNSELIPEKHCVIFILNDGSAKKVNVNGCFNADSIKKRLIRRLPHELLATN
SNGEVTKMVQDYDVFVLDYTKNVLHLLYDVELVTICHANDRVEKNRLIFVSKDQTPSD
KAISTSKKLYLRTLSALSQVGPSSSNLLAQNKGISHNNAEGKLRIDNTEKDRIRQIFN
QRPPSEFISTNLAGYFPHTDMKRLQKTMRESFRHSARLSIAQRRPLSAESNNIGDILL
KHSNAVDMALLQGLDQTRLSSKLDTTKIPKLAHKRPEDNDAISNQLELLSVESGEEED
HDFFGEDSDIVSLPTKIATPKNWLKGACIGSGSFGSVYLGMNAHTGELMAVKQVEIKN
NNIGVSTDNNKQANSDENNEQEEQQEKIEDVGAVSHPKTNQNIHRKMVDALQHEMNLL
KELHHENIVTYYGASQEGGNLNIFLEYVPGGSVSSMLNNYGPFEESLITNFTRQILIG
VAYLHKKKIIHRDIKGANILIDIKGCVKITDFGISKKLSPLNKKQNKRASLQGSVFWM
SPEVVKQSATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIGTNTTPEIPSWAT
SEGKNFLRKAFELDYQYRPGALELLQHPWLDAHII"
gene complement(803787..804443)
/gene="Nmd4"
/locus_tag="R008_L12676"
mRNA complement(803787..804443)
/gene="Nmd4"
/locus_tag="R008_L12676"
/product="Nmd4p"
CDS complement(803787..804443)
/gene="Nmd4"
/locus_tag="R008_L12676"
/note="Similar to YLR363C Protein interacting with Nam7p,
may be involved in the nonsense-mediated mRNA decay
pathway"
/codon_start=1
/product="Nmd4p"
/protein_id="EWG84569.1"
/translation="MTQYNFIIDASAFEKGLGNIKRWCSDCTEAVTLNFYIPTFTLNE
LDFLQQRRKSFAARESLKFIDRLDDSKFANLKVFIEFPEVLDIILWSDVMEHNDSSGK
MNIAKLPKRLKNLLKSCIYKCYLEGNEGLHWFLISEDPQIREMAMQCNIPSCSIVDVD
SILSKDMNDKSFRESEKFNNMMLKNGTKEESENGREIIRTNFNKTVYASRGTGELWSP
"
gene 804753..805010
/locus_tag="R008_L12681"
mRNA 804753..805010
/locus_tag="R008_L12681"
/product="hypothetical protein"
CDS 804753..805010
/locus_tag="R008_L12681"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84570.1"
/translation="MPQKPLKVTKKAKDPRRVTKKQKNLRKAAPLQLKSKKKSLQHLK
KLKKSSSLTETTERLVASKVGHLELLRGTRKELEKGKKNSK"
gene 805354..805683
/locus_tag="R008_L12686"
mRNA 805354..805683
/locus_tag="R008_L12686"
/product="hypothetical protein"
CDS 805354..805683
/locus_tag="R008_L12686"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84571.1"
/translation="MSAFVTKAEEMIKSHPYFQLSASWCPDCVYANSIWNKLNVQDKV
FVFDIGSLPRNEQEKWRIAFQKVVGSRNLPTIVVNGKFWGTESQLHRFEAKGTLEEEL
TKIGLLP"
gene complement(806816..806948)
/locus_tag="R008_L12701"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 807753..808628
/gene="Rps22b"
/locus_tag="R008_L12716"
mRNA join(807753..807885,808369..808628)
/gene="Rps22b"
/locus_tag="R008_L12716"
/product="Rps22bp"
CDS join(807753..807885,808369..808628)
/gene="Rps22b"
/locus_tag="R008_L12716"
/note="Similar to YLR367W 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps22Ap and
has similarity to E. coli S8 and rat S15a ribosomal
proteins'"
/codon_start=1
/product="Rps22bp"
/protein_id="EWG84572.1"
/translation="MTRSSVLADALNAINNAEKTGKRQVLLRPSSKVIIKFLQVMQKH
GYIGEFEYIDDHRSGKIVVQLNGRLNKCGVISPRFNVKIGDIEKWTANLLPARQFGYV
ILTTSAGIMDHEEARRKHVSGKILGFVY"
gene 808851..810647
/gene="Mdm30"
/locus_tag="R008_L12726"
mRNA 808851..810647
/gene="Mdm30"
/locus_tag="R008_L12726"
/product="Mdm30p"
CDS 808851..810647
/gene="Mdm30"
/locus_tag="R008_L12726"
/note="Similar to YLR368W 'F-box component of an SCF
ubiquitin protein ligase complex; associates with and is
required for Fzo1p ubiquitination and for mitochondria
fusion; stimulates nuclear export of specific mRNAs;
promotes ubiquitin-mediated degradation of Gal4p in some
strains'"
/codon_start=1
/product="Mdm30p"
/protein_id="EWG84573.1"
/translation="MTKRRNLFMVGSSFTIDHLPPEIWLCISKLLGTSDLHNLCLINR
RLYLTITSDEIWKRRCYDRWINRENLDILTGNDYDSIPVSQWYSYYLRRAKWENKIFC
LLWGLTEETNPQHFREKYLHILQFRHYKLATFLHRIIKQGYIPDKRPLDLITYANYLL
KNVRHKYVFPLFYPTNAAELKNLNNMASRDAEMIYLRLSAIDTSFDDLLDAREFILNG
ICSDLLQKYKKIEEFLKLRPVTRVSKLISISTDYLDCFTQPHDSVGQTNDRATGRELH
REDFMLLRVYSREGRGYKTIILAIIQAITKRYNVDSYLARDHLVVSEPDFPDGRAFVT
VNEDFQPYIFDKEDLLSVWSNDFHNAENFESAVLPALLEPISIQHLLTEFFRELLRCK
PRPFEGYPNRAHGLRDMFPYGKVEVPRDVTMYFAFIYDLFDGMFESGMTSLRGQMLRD
LLNYVNANNFGDLNIIIGQNALKEPNDCWSNKRDYVLLDDNNKIGYFYTDIETEDTLC
ALNQYEVDGKVFITTIDILGDIRVRLAEGLTPFQGDNDKLWESFSSVVPRTDWGLFFK
GYDKERRRMQLNPYIEEKLSNLANDEQPLHNL"
gene 810863..812836
/gene="Ssq1"
/locus_tag="R008_L12731"
mRNA 810863..812836
/gene="Ssq1"
/locus_tag="R008_L12731"
/product="Ssq1p"
CDS 810863..812836
/gene="Ssq1"
/locus_tag="R008_L12731"
/note="Similar to YLR369W Mitochondrial hsp70-type
molecular chaperone, required for assembly of iron/sulfur
clusters into proteins at a step after cluster synthesis,
and for maturation of Yfh1p, which is a homolog of human
frataxin implicated in Friedreich's ataxia"
/codon_start=1
/product="Ssq1p"
/protein_id="EWG84574.1"
/translation="MLKSGRLNFLKLNINSRLLYSTNPQLAKKVIGIDLGTTNSAVAY
IRDSNDKKSATIIENDEGQRTTPSIVAFDVKSSPQNKDQMKTLVGMAAKRQNAINSEN
TFFATKRLIGRAFNDKEVQRDMAVMPYKIVKCESNGQAYLSTSNGLIQSPSQIASILL
KYLKQTSEEYLGEKVNLAVITVPAYFNDSQRQATKDAGKLAGLNVLRVINEPTAAALS
FGIDDKRNNGLIAVYDLGGGTFDISILDIEDGVFEVRATNGDTHLGGEDFDNVIVNYI
IDTFIHENPEITREEITKNRETMQRLKDVSERAKIDLSHVKKTFIELPFVYKSKHLRV
PMTEEELDNMTLSLINRTIPPVKQALKDADIEPEDIDEVILVGGMTRMPKIRSVVKDL
FGKSPNSSVNPDETVALGAAIQGGILSGEIKNVLLLDVTPLTLGIETFGGAFSPLIPR
NTTVPVKKTEIFSTGVDGQTGVDIKVFQGERGLVRNNKLIGDLKLTGITPLPKGIPQI
YVTFDIDADGIINVSAAEKSSGKKQSITVIPNSGLSEEEIAKLIEEANANRAQDNLIR
QRLELISKADIMISDTESLFKRYEKLISSEKEYSNIVEDIKALRQAIKNFKANENDMS
IDVNGIKKATDALQGRALKLFQSATKNQQNQGK"
gene complement(813030..813566)
/gene="Arc18"
/locus_tag="R008_L12736"
mRNA complement(813030..813566)
/gene="Arc18"
/locus_tag="R008_L12736"
/product="Arc18p"
CDS complement(813030..813566)
/gene="Arc18"
/locus_tag="R008_L12736"
/note="Similar to YLR370C Subunit of the ARP2/3 complex,
which is required for the motility and integrity of
cortical actin patches"
/codon_start=1
/product="Arc18p"
/protein_id="EWG84575.1"
/translation="MPAYHSTFPVDPNTDRMVGNFALLPLNTKFRGPAYPSNSDYDII
DECLDLFRANSFFKNFEIKSPADRVLIYGILFINDCLAHLKITTSFNEAVKVLTNVAL
DNFTLPGTPGFPLNNVYQVPVQDHNSMDLLKTYIQQFRQELAMRLLERVYSSTDSKEY
PSKFWLAFTRRRFMNKSL"
gene 814026..818102
/gene="Rom2"
/locus_tag="R008_L12741"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 818681..819718
/gene="Sur4"
/locus_tag="R008_L12751"
mRNA 818681..819718
/gene="Sur4"
/locus_tag="R008_L12751"
/product="Sur4p"
CDS 818681..819718
/gene="Sur4"
/locus_tag="R008_L12751"
/note="Similar to YLR372W 'Elongase, involved in fatty
acid and sphingolipid biosynthesis; synthesizes very long
chain 20-26-carbon fatty acids from C18-CoA primers;
involved in regulation of sphingolipid biosynthesis'"
/codon_start=1
/product="Sur4p"
/protein_id="EWG84576.1"
/translation="MNTTTSTVIAAVADQFQSLNSSSSCFLKVHVPSIENPFGIELWP
IFSKVFEYFSGYPAEQFEFIHNKTFLANGYHAVSIIIVYYIIIFGGQAILRALNASPL
KFKLLFEIHNLFLTSISLVLWLLMLEQLVPMVYHNGLFWSICSKEAFAPKLVTLYYLN
YLTKFVELIDTVFLVLRRKKLLFLHTYHHGATALLCYTQLIGRTSVEWVVILLNLGVH
VIMYWYYFLSSCGIRVWWKQWVTRFQIIQFLIDLVFVYFATYTFYAHKYLDGILPNKG
TCYGTQAAAAYGYLILTSYLLLFISFYIQSYKKGGKKTVKKESEVSGSVASGSSTGVK
TSNTKVSSRKA"
gene complement(819989..822694)
/gene="Vid22"
/locus_tag="R008_L12756"
mRNA complement(819989..822694)
/gene="Vid22"
/locus_tag="R008_L12756"
/product="Vid22p"
CDS complement(819989..822694)
/gene="Vid22"
/locus_tag="R008_L12756"
/note="Similar to YLR373C 'Glycosylated integral membrane
protein localized to the plasma membrane; plays a role in
fructose-1,6-bisphosphatase (FBPase) degradation; involved
in FBPase transport from the cytosol to Vid (vacuole
import and degradation) vesicles'"
/codon_start=1
/product="Vid22p"
/protein_id="EWG84577.1"
/translation="MRAMDTQVQSAERGLVLPPMNSTVSSATAATTATNTDTDTDGDR
DEEKESLAEDGSEWVPAYMLTRDRSRYLGHFLGVDKMLEAVKCKYCGVIIRRQGNSIN
MAEASQTHLWSTHKIDPNANYYSGWTGVEAGSTFMVRPPLKNHQGGSATTNSIANLLE
IDEDFLKRTREKEMALPLVQSLAIIIASENLPLSFVDNTAVRLLINQNANSLSFIDHD
LILNAIRSIAYNLDRIIQRTALRNNSDLSLIIDKNYLLMDPTDRSNQLSNRLKNQLFE
MQKINFFSLSHSVWNNTISILSIQYYDDFHSQVKTLPLIIQNLHEYNNDPKLSIPAQL
LKISQELPGLQNTVISITLPRSQIVDLLNVMDSQPFFPNTYTNAKNYYHNCIISIINS
AILPLFGTAKSADITHPRQSSFSKEPLTLLDSLIDLSNIDISNSIFSRINSFLDDLQS
NSWQLDKFRSLCEKFGFEFVCSKFDLSRYSTATVSLQTFLNLRPIIEEYQSSIQIEKF
NEIDFQIIDYLLITLNSINRILKFFTSSKSLNFTYVLFAIMSIEKHLLSTLGSLQFQR
LIAPFETFLSKIQEFKTILFSDDMNLLAMFLCPAILFEREVLEYSFHTISLSEIVDKL
STSIFSLLKRFLNLHTIGNVNNSHNTSNHSNMNIHTDNQTNNINNRSGNNSDNNDNEH
DNDNVNHSNSNTPASRIDIDPTGGENSVLPEQQPQNSNNNLSFGSLSDTHHLSDSTIS
KEIDSIFLQIIQEDLYDYLSTVNSIVPISYRSYCEQSNFIRDSGRFKKRIITEDSIIG
ELEQPMNFIEELLDIHVPVCNAFWSQYLDNDAGPIIRILFKIMQCQSSSSIRGEYSFL
NDFIPRVHPDLTQEIIKIKLFNDQFVASKVDYDLDTLQTASQYLS"
gene 823023..824054
/gene="Stp3"
/locus_tag="R008_L12766"
mRNA 823023..824054
/gene="Stp3"
/locus_tag="R008_L12766"
/product="Stp3p"
CDS 823023..824054
/gene="Stp3"
/locus_tag="R008_L12766"
/note="Similar to YLR375W Zinc-finger protein of unknown
function, possibly involved in pre-tRNA splicing and in
uptake of branched-chain amino acids"
/codon_start=1
/product="Stp3p"
/protein_id="EWG84578.1"
/translation="MSNANNSAMNHITLPPISSFDNLIKAAERQYNGEASSASTHPTL
PNMNISNGSGSAGASSSMLSYQLLPHSNDVSRSNSSSSFLPSVQQPTEGSASASETSS
SASPSRSISPILKVAGPSSVGGAGVSTPHSTKINKPRKKKQCPICRNFYANLTTHKAT
HLTPEDRPHKCPICHRGFARNNDLLRHKKRHWKDEILSQSGVLSNHNDGKGGSVSPND
DDTHEKMTPMNSVTDYAQLKSLHQIKGTFKCPFNSTLIQLDMDMYPYKLKPLNFETSN
CHQTGVFSRCDTFKNHLKALHFEYPPGTKKKDRNVVPGRCKHCGLKFENVDVWLNEHV
GKQCGYKYH"
gene complement(824154..824882)
/gene="Psy3"
/locus_tag="R008_L12771"
mRNA complement(824154..824882)
/gene="Psy3"
/locus_tag="R008_L12771"
/product="Psy3p"
CDS complement(824154..824882)
/gene="Psy3"
/locus_tag="R008_L12771"
/note="Similar to YLR376C 'Protein involved in a Rad51p-,
Rad54p-dependent pathway for homologous recombination
repair; deletion results in a mutator phenotype; deletion
increases sensitivity to anticancer drugs oxaliplatin and
cisplatin but not mitomycin C'"
/codon_start=1
/product="Psy3p"
/protein_id="EWG84579.1"
/translation="MEVLKNIRIYPLSNFITSTKNYINLPNELRNLISEEQESKLGFL
HIIESDFKPSVALQKLVNCTTGDEKILIIDIVSIWSQQKQRQHGAIYMNSLSCINITG
LIVFLELLYDSPMDALRRCQVDNFNFQLRGILIDNLSFLNFESDKNYDVINLSKFEKL
FKILRKLREFLGCWIITKSFPTDFYNGIENTLVDKWSIKRKSGVTLYPTKLPDSYMKG
MDLIIYREVVDGRPQYRRIAALEE"
gene complement(825077..826123)
/gene="Fbp1"
/locus_tag="R008_L12776"
mRNA complement(825077..826123)
/gene="Fbp1"
/locus_tag="R008_L12776"
/product="Fbp1p"
CDS complement(825077..826123)
/gene="Fbp1"
/locus_tag="R008_L12776"
/note="Similar to YLR377C 'Fructose-1,6-bisphosphatase,
key regulatory enzyme in the gluconeogenesis pathway,
required for glucose metabolism; undergoes either
proteasome-mediated or autophagy-mediated degradation
depending on growth conditions; interacts with Vid30p'"
/codon_start=1
/product="Fbp1p"
/protein_id="EWG84580.1"
/translation="MPTLVNGPRRDSTEGFDTDIITLPRFIIEHQKQFKNATGDFTLV
LNALQFAFKFVSHTIRRAELVNLVGLAGASNFTGDQQKKLDVLGDEIFINAMRASGII
KVLVSEEQEDLIVFPTNTGSYAVCCDPIDGSSNLDAGVSVGTIASIFRLLPDSSGTIN
DVLRCGKEMVAACYAMYGSSTHLVLTLGDGVDGFTLDTNLGEFILTHPNLRIPPQKAI
YSINEGNTLYWNETIRTFIEKVKQPQADNNNKPFSARYVGSMVADVHRTFLYGGLFAY
PCDKKSPNGKLRLLYEAFPMAFLMEQAGGKAVNDRGERILDLVPSHIHDKSSIWLGSS
GEIDKFLDHIGKSP"
gene 826706..826801
/locus_tag="R008_L12786"
tRNA 826706..826801
/locus_tag="R008_L12786"
/product="tRNA-Lys"
/note="TK(UUU)L"
gene complement(827064..828506)
/gene="Sec61"
/locus_tag="R008_L12791"
mRNA complement(827064..828506)
/gene="Sec61"
/locus_tag="R008_L12791"
/product="Sec61p"
CDS complement(827064..828506)
/gene="Sec61"
/locus_tag="R008_L12791"
/note="Similar to YLR378C 'Essential subunit of Sec61
complex (Sec61p, Sbh1p, and Sss1p); forms a channel for
SRP-dependent protein import and retrograde transport of
misfolded proteins out of the ER; with Sec63 complex
allows SRP-independent protein import into ER'"
/codon_start=1
/product="Sec61p"
/protein_id="EWG84581.1"
/translation="MSSNRVLDLFKPFESFLPEVIAPERKVPYNQKLIWTGVSLLIFL
ILGQIPLYGIVSSETSDPLYWLRAMLASNRGTLLELGVSPIITSSMIFQFLQGTQLLQ
IRPESKQDRELFQIAQKVCAIILILGQALVVVMTGNYGAPSDLGLPICLLLIFQLMFA
SLIVMLLDELLSKGYGLGSGISLFTATNIAEQIFWRAFAPTTVNSGRGKEFEGAVIAF
FHLLAVRKDKKRALVEAFYRTNLPNMFQVLMTVAIFLFVLYLQGFRYELPIRSTKVRG
QIGIYPIKLFYTSNTPIMLQSALTSNIFLISQILFQKYPTNPLIRLIGVWGIRPGTQG
PQMALSGLAYYIQPLMSLSEALLDPIKTIVYITFVLGSCAVFSKTWIEISGTSPRDIA
KQFKDQGMVINGKRETSIYRELKKIIPTAAAFGGATIGALSVGSDLLGTLGSGASILM
ATTTIYGYYEAAAKEGGFTKNLVPGFSDLM"
gene 829606..830832
/gene="Csr1"
/locus_tag="R008_L12801"
mRNA 829606..830832
/gene="Csr1"
/locus_tag="R008_L12801"
/product="Csr1p"
CDS 829606..830832
/gene="Csr1"
/locus_tag="R008_L12801"
/note="Similar to YLR380W 'Phosphatidylinositol transfer
protein with a potential role in regulating lipid and
fatty acid metabolism under heme-depleted conditions;
interacts specifically with thioredoxin peroxidase; may
have a role in oxidative stress resistance'"
/codon_start=1
/product="Csr1p"
/protein_id="EWG84582.1"
/translation="MSFDRQLTEDQEVVLKQIWTHLFHLWQVPVDGTHIFPNNSLHSS
STPAKKKKSSWFSKLQSSDHTQDSSEAAEAAHLYEKGKIHKALANLDPQTTKKQFWHD
IKNETPDATILKFIRARKWNADKTIAMLGHDLYWRKDTINKIINGGERAVYENNETGV
IKNLELQKATIQGYDNDMRPVILVRPRLHHSSDQTEQELEKFSLLVIEQSKLFFKENY
PASTTILFDLNGFSMSNMDYAPVKFLITCFEAHYPESLGHLLIHKAPWIFNPIWNIIK
NWLDPVVASKIVFTKNIDELHKFIQPQYIPRYLGGENDNDLDHYTPPDGSLDVHLKDT
ETRAMIEKEREELVEQFLTVTAQWIEHQPLNDPAYIQLQEKRVQLSTALCENYSKLDP
YIRSRSVYDYNGSLKV"
gene 831047..833248
/gene="Ctf3"
/locus_tag="R008_L12806"
mRNA 831047..833248
/gene="Ctf3"
/locus_tag="R008_L12806"
/product="Ctf3p"
CDS 831047..833248
/gene="Ctf3"
/locus_tag="R008_L12806"
/note="Similar to YLR381W 'Outer kinetochore protein that
forms a complex with Mcm16p and Mcm22p; may bind the
kinetochore to spindle microtubules'"
/codon_start=1
/product="Ctf3p"
/protein_id="EWG84583.1"
/translation="MSLILDDIILSLTNANERTPPQALKTTLSLLYEKSKQYGLSSPQ
LQALVRLLCETSIIDTVTKVYIVENCFLPDGYLTKELLLEIINHLGTPTVFSRYRIQT
PPILQSALCKWLVHVYFLFPVHSEREHNISSSIWLHLWQFSFLQKWITPLVIWQATTP
VDVKPWKLSIIKRCAMNPGYRDAPASATLILQRFQCLVGASSQITESIITINCNRKTL
KSHRNLKLDAHFLSILKRILSRAHPANFPADTVQNTIDMYLSEIHQLGADSIYPLRLQ
SLPEYVPSDSTVSLWDVTSLEQLAQNWPQLHIPNDVDYMMKPSLNSNVLLPRKVMSRD
SLKHLYSSIILIKNSRDESSSPYEWCIWQLKRCFAHQIETPQEVIPIIISVSSMDNKL
SSRIIQTFCNLKYLKLDELTLKKVCGGILPLWKPELISGTREFFVKFMASIFMWSTRD
GHDNNCTFSETCFYVLQMITNWVLDDKLIALGLTLLHDMQSLLTLDKIFNNATSNRFS
TMAFISSLDILTQLSKQTKSDYAIQYLIVGPDIMNKVFSSDDPLLLSAACRYLVATKN
KLMQYPSTNKFVRMQNQYIMDLTNYLYRNKVLSSKSLFGVPPDFFKQILENLYVPTAD
FKNAKFFTITGIPALFYICIIILRRLETAENTKIKFTSGIINEETFNNFFRVHHDEIG
QHGWIKGVNNIHDLRVKILMHLSNTANPYRDIAAFLFTYLKSLSKYSVQNS"
gene complement(833395..836079)
/gene="Nam2"
/locus_tag="R008_L12811"
mRNA complement(833395..836079)
/gene="Nam2"
/locus_tag="R008_L12811"
/product="Nam2p"
CDS complement(833395..836079)
/gene="Nam2"
/locus_tag="R008_L12811"
/note="Similar to YLR382C 'Mitochondrial leucyl-tRNA
synthetase, also has a direct role in splicing of several
mitochondrial group I introns; indirectly required for
mitochondrial genome maintenance'"
/codon_start=1
/product="Nam2p"
/protein_id="EWG84584.1"
/translation="MLSRPSSRFLSTKRGPGPAVKKLIAIGEKWKQKTTRGLPKQDTP
NSGSKYILCQFPYPSGALHIGHLRVYVISDSLNRFYKQKGYNVIHPMGWDAFGLPAEN
AAIERSINPAIWTRDNIAKMKQQMQSMLANFDWDREITTCDPEYYKFTQWIFLKLFEN
GLAYRKEAEINWDPVDMTVLANEQVDAQGRSWRSGAIVEKKQLKQWFLGITKFAPKLK
KHLNQLKDWPSNVKQMQKNWIGESVGAELVFKVADPKFENLIVFTTRPETLFAVQYVA
LALDHPIVQKYCEEMPDLKEFIQKSDQLPNDTKEGFQLPNIKAVNPLTKEEVPIFAAP
YVVSSYGSAPSAVMGCPGHDNRDFEFWQTNCPGEHIKTCIAPFFDDASKVTEQERQRI
IDTVPFTSTDGVLTKECGEHSGVLTAVARKSIMGMLNSEGLSKSVVRYKIRDWLISRQ
RYWGTPIPIIHCDNCGPVPVPESDLPVKLPELEGLDTKGNPLSTIDEFVNVACPSCGS
PAKRETDTMDTFIDSSWYYFRFLDPKNTSKPFDREIASKNMPVDIYIGGVEHAILHLL
YSRFIAKFLGSINAWSDPAGIFEPFKKLVTQGMVQGKTYVDPDSGKFLKPDELTFVND
SPDGNTVIIKSNGKVPVVSYEKMSKSKYNGADPNECILRHGPDATRAHILFQSPIADA
LNWDESKIVGIERWLQKVLHLTKNILSLEKDLAISKDYKTPTDLNDAEVKFHNDFQRF
LKSITESFEVNLSLNTVISDYMKLTNILESALKKGEVRNEMIVQNLQKLVTVIYPAVP
SISEEAAEMINSQMEWNQYRWPEVERTTESKFKKFQIVVNGRVKFMYTADKNFLKLGR
DAVIETLMNLPEGRMYLMNKKIKKFVMKFNVISFLFHK"
gene 836617..839971
/gene="Smc6"
/locus_tag="R008_L12816"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(840200..844249)
/gene="Iki3"
/locus_tag="R008_L12821"
mRNA complement(840200..844249)
/gene="Iki3"
/locus_tag="R008_L12821"
/product="Iki3p"
CDS complement(840200..844249)
/gene="Iki3"
/locus_tag="R008_L12821"
/note="Similar to YLR384C 'Subunit of Elongator complex,
which is required for modification of wobble nucleosides
in tRNA; maintains structural integrity of Elongator;
homolog of human IKAP, mutations in which cause familial
dysautonomia (FD)'"
/codon_start=1
/product="Iki3p"
/protein_id="EWG84585.1"
/translation="MVEHDKSGSKRQELRSNMRNLITLNKGKFKPTASTAEGDEDDLS
FTLLDSVFDTLSDSITCVLGSTDIGAIEVQQFMKDGSRNVLASFNIQTFDDKLLSFVH
FADINQLVFVFEQGDIITATYDPVSLDPAETLIEIMGTIDNGIAAAQWSYDEETLAMV
TKDRNVVVLSKLYEPISEYHLEVDDLKISKHVTVGWGKKETQFRGKGARAMEREALAS
LKASGLVGNQLRDPTMPYMVDTGDVTALDSHEITISWRGDCDYFAVSSVEEVPDEDDE
TKSIKRRAFRVFSREGQLDSASEPVTGMEHQLSWKPQGSLIASIQRKTDLGEEDSVDV
IFFERNGLRHGEFDTRLPLDEKVESVCWNSNSEALAVVLANRIQLWTSKNYHWYLKQE
LYASDISYVKWHPEKDFTLMFSDAGFINIVDFAYKMAQGPTLEPFDNGTSLVVDGRTV
NITPLALANVPPPMYYRDFETPGNVLDVACSFSNEIYAAINKDVLIFAAVPSIEEMKK
GKHPSIVCEFPKSEFTSEVDSLRQVAFINDSIVGVLLDTDNLSRIALLDIQDITQPTL
ITIVEVYDKIVLLRSDFDYNHLVYETRDGTVCQLDAEGQLMEITKFPQLVRDFRVKRV
HNTSAEDDGNWSAESSELVAFGITNNGKLFANQVLLASAVTSLEITDSFLLFTTAQHN
LQFVHLNSTDFKPLPLVEEGVEDERVRAIERGSILVSVIPSKSSVVLQATRGNLETIY
PRIMVLAEVRKNIMAKRYKEAFIVCRTHRINLDILHDYAPELFIKNLEVFINQIGRVD
YLNLFISCLSEDDVTKTKYKETLYSGISKSFGMEPAPLTEMQIYMKKKMFDPKTSKVN
KICDAVLNVLLSNPEYKKKYLQTIITAYASQNPQNLPAALKLISELENSEEKDSCVTY
LCFLQDVNVVYKSALSLYDVSLALLVAQKSQMDPREYLPFLQELQDNEPLRRKFLIDD
YLGNYEKALEHLSEIDKDGNVSEEVIDYVESHDLYKHGLALYRYDSEKQNVIYNIYAK
HLSSNQMYTDAAVAYEMLGKFKEAMGAYQSAKRWREAMSIAVQKFPEEVESVAEELIS
SLTFEHRYVDAADIQLEYLDNVKEAVALYCKAYRYDIASLVAIKAKKDELLEEVVDPG
LGEGFGIIAELLADCKGQINSQLRRLRELRAKKEENPYAFYGQETEQADDVSVAPSET
STQESFFTRYTGKTGGTAKTGASRRTAKNKRREERKRARGKKGTIYEEEYLVQSVGRL
IERLNQTKPDAVRVVEGLCRRNMREQAHQIQKNFVEVLDLLKANVKEIYSISEKDRER
VNENGEVYYIPEIPVPEIHDFPKSHIVDF"
gene complement(844341..844739)
/gene="Swc7"
/locus_tag="R008_L12826"
mRNA complement(844341..844739)
/gene="Swc7"
/locus_tag="R008_L12826"
/product="Swc7p"
CDS complement(844341..844739)
/gene="Swc7"
/locus_tag="R008_L12826"
/note="Similar to YLR385C Protein of unknown function,
component of the Swr1p complex that incorporates Htz1p
into chromatin"
/codon_start=1
/product="Swc7p"
/protein_id="EWG84586.1"
/translation="MDCPSNIVLLLLQLVLQRQQTLAHRDKSVDLQTLLKDPVIDNDV
LVEFKTHKLVQLYGPQYCRDISLRGLKTMVTDIFANGIPRNAQSSGNDQPVTVVDLAN
YYYMQRINELQNTELPQLKEALLTRLEHMI"
gene 844977..847619
/gene="Vac14"
/locus_tag="R008_L12831"
mRNA 844977..847619
/gene="Vac14"
/locus_tag="R008_L12831"
/product="Vac14p"
CDS 844977..847619
/gene="Vac14"
/locus_tag="R008_L12831"
/note="Similar to YLR386W 'Protein involved in regulated
synthesis of PtdIns(3,5)P(2), in control of trafficking of
some proteins to the vacuole lumen via the MVB, and in
maintenance of vacuole size and acidity; interacts with
Fig4p; activator of Fab1p'"
/codon_start=1
/product="Vac14p"
/protein_id="EWG84587.1"
/translation="MEKSIAKGLSDKLYEKRKAAALELEKLVKQCVLEGDYDRIDKII
DELCRDYAYALHQPMARNAGLMGLAATAIALGINDVGRYLRNILPPVLACFGDQNDQV
RFYACESLYNIAKIAKGEILVYFNEIFDVLCKISADTENSVRGAAELLDRLIKDIVAE
RASNYISIVNNGSHGLLPAIKTDPISGDVYQEEYEQDNQLAFSLPKFIPLLTERIYAI
NPDTRVFLVDWLKVLLNTPGLELISYLPSFLGGLFTFLGDSHKDVRTVTHTLMDSLLH
EVDRISKLQTEIKMKRLERLKMLEDKYNNSSTPTKKADGALIAEKKKTLMTALGGLSK
PLSMETDDTKLSNTNETDDERHLTSQEQLLDSEATSQEPLRDGEEYIPGQDINLNFPE
VITVLVNNLASSEAEIQLIALHWIQVILSISPNVFIPFLSKILSVLLKLLSDSDPHIT
EIAQLVNGQLLSLCSSYVGKETDGKIAYGPIVNSLTLQFFDSRIDAKIACLDWLILIY
HKAPNQILKHNDSMFLTLLKSLSNRDSVLIEKALSLLQSLCSDSNDNYLRQFLQDLLT
LFKRDTKLVKTRANFIMRQISSRLSPERVYKVISSILDNYNDTTFVKMMIQILSTNLI
TSPEMSSLRNKLRTCEDGMFFNSLFKSWCPNPVSVISLCFVAENYELAYTVLQTYANY
ELKLNDLVQLDILIQLFESPVFTRMRLQLLEQQKHPFLHKCLFGILMIIPQSKAFETL
NRRLNSLNIWTSQSYVMNNYIRQRENSNFCDSNSDISQRSVSQSKLHFQELINHFKAV
SEEDEYSSDMIRLDHGANNKSLLLGSFLDGIDEDKQEIVTPISPMNEAINEEMESPND
SSSVILKDSGSLPFNRNVSDKLKK"
gene complement(847723..849021)
/gene="Reh1"
/locus_tag="R008_L12836"
mRNA complement(847723..849021)
/gene="Reh1"
/locus_tag="R008_L12836"
/product="Reh1p"
CDS complement(847723..849021)
/gene="Reh1"
/locus_tag="R008_L12836"
/note="Similar to YLR387C 'Cytoplasmic 60S subunit
biogenesis factor, associates with pre-60S particles;
similar to Rei1p and shares partially redundant function
in cytoplasmic 60S subunit maturation; contains dispersed
C2H2 zinc finger domains'"
/codon_start=1
/product="Reh1p"
/protein_id="EWG84588.1"
/translation="MSSTFFTCNCCVIQFKTSDLQRYHMKTEWHRYNLKRRIANLPPI
GAEQFAEKLQISEKEQAENQVDEFGFPVLKPVMNQSNALPQKQKKPIKSKRGRKVGTN
LLKRKDRDIAKEKQNRSVSPSGSISSQLSNLTVGTENTNTDYGEDTVSEYGFTSDSNY
EYATSDEELDIADKPSDKENEKITITECIYCGKDNKEVERNVKHMFSEHGLFIPERSY
LIDLNGLLEFLIKMIVIDHNCLCCNFHGSGLESIRAHMASKRHCRLPYETKEERQLFA
PFYDFTYDDHSISKNLQNDRAITSKLSSVYGAKNDEEDGEVDITLVSSENDINANYTT
VSIDESGLELTLPTGARLGHRAGQRYYRQNLPSQPNPNESRRTITAADRRMVSGVTEK
QYKKGMKKMQQLEKNAINTQIRREIKRVNFQTHYRDELLQ"
gene 849999..850169
/gene="Rps29a"
/locus_tag="R008_L12846"
mRNA 849999..850169
/gene="Rps29a"
/locus_tag="R008_L12846"
/product="Rps29ap"
CDS 849999..850169
/gene="Rps29a"
/locus_tag="R008_L12846"
/note="Similar to YLR388W 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps29Bp and
has similarity to rat S29 and E. coli S14 ribosomal
proteins'"
/codon_start=1
/product="Rps29ap"
/protein_id="EWG84589.1"
/translation="MAHENVWFSHPRRYGKGSRQCRVCSSHTGLIRKYGLNICRQCFR
EKANDIGFNKFR"
gene 850527..850729
/locus_tag="R008_L12851"
ncRNA 850527..850729
/ncRNA_class="snoRNA"
/locus_tag="R008_L12851"
/product="SNR34"
gene complement(850924..854007)
/gene="Ste23"
/locus_tag="R008_L12856"
mRNA complement(850924..854007)
/gene="Ste23"
/locus_tag="R008_L12856"
/product="Ste23p"
CDS complement(850924..854007)
/gene="Ste23"
/locus_tag="R008_L12856"
/note="Similar to YLR389C 'Metalloprotease involved, with
homolog Axl1p, in N-terminal processing of pro-a-factor to
the mature form; expressed in both haploids and diploids;
member of the insulin-degrading enzyme family'"
/codon_start=1
/product="Ste23p"
/protein_id="EWG84590.1"
/translation="MGVSLLASSSAFVTKPLLTQLVHFSPISLNFTVRRFKPFTCLSR
YYTTNPYNMTSNFKTFNLDFLKPDLDERSYRFIELPNKLKALLIQDPKADKAAASLDV
NIGAFEDPENLPGLAHFCEHLLFMGSEKFPDENEYSSYLSKHGGSSNAYTASQNTNYF
FEVNHQHLFGALDRFSGFFSCPLFNKDSTDKEINAVNSENKKNLQNDIWRIYQLDKSL
TNTKHPYHKFSTGNIETLGTLPKENGLNVRDELLKFHKNFYSANLMKLCILGREDLDT
LSNWTYDLFKDVANNGREVPLYAEPIMQPEHLQKIIQVRPVKDLKKLEISFTVPDMEE
HWESKPPRILSHLIGHEGSGSLLAHLKKLGWANELSAGGHTVSKGNAFFAVDIDLTDN
GLTHYRDVIVLIFQYIEMLKNSLPQKWIFNELQDISNATFKFKQAGSPSSTVSSLAKC
LEKDYIPVSRILAMGLLTKYEPDLLTQYTDALVPENSRVTLISRSLETDSAEKWYGTA
YKVVDYPADLIKNMKSPGLNPALTLPRPNEFVSTNFKVHKIDGIKPLDEPVLLLSDDV
SKLWYKKDDRFWQPRGYIYLSFKLPHTHASIINSMLSTLYTQLANDALKDLQYDAACA
DLRISFNKTNQGLAITASGFNEKLIILLTRFLQGVNSFEPKKDRFEILKDKTIRHLKN
LLYEVPYSQMSNYYNAIINERSWSTAEKLQVFEKLTFEQLINFIPTIYEGVYFETLIH
GNIKHEEALEVDSLIKSLIPNNIHNLQVSNNRLRSYLLPKGKTFRYETALKDSRNVNS
CIQHVTQLDVYSEDLSALSGLFAQLIHEPCFDTLRTKEQLGYVVFSSSLNNHGTANIR
ILIQSEHTTPYLEWRINNFYETFGQVLRDMPEEDFEKHKEALCNSLLQKFKNMAEESA
RYTAAIYLGDYNFTHRQKKAKLVANITKQQMIDFYENYIMSENASKLILHLKSQVENK
ELNENELDTAKYPTGQLIEDVGAFKSTLFVAPVRQPMKDFEISAPPKLNNSSESD"
gene 854413..854751
/gene="Ecm19"
/locus_tag="R008_L12871"
mRNA 854413..854751
/gene="Ecm19"
/locus_tag="R008_L12871"
/product="Ecm19p"
CDS 854413..854751
/gene="Ecm19"
/locus_tag="R008_L12871"
/note="Similar to YLR390W 'Putative protein of unknown
function; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies'"
/codon_start=1
/product="Ecm19p"
/protein_id="EWG84591.1"
/translation="MDWLKNTTIVVLFSHSTDKSIKHKKRQVQCNMRKNTLDMVTIGI
ACLVGVYTGTRFFEPIVIDRLRKDGNLRTDIPIPEYDEDGNLLKVTPSLSSTPAAPPT
PPTPPTPPQQ"
gene 855071..855787
/gene="Ccw14"
/locus_tag="R008_L12876"
mRNA 855071..855787
/gene="Ccw14"
/locus_tag="R008_L12876"
/product="Ccw14p"
CDS 855071..855787
/gene="Ccw14"
/locus_tag="R008_L12876"
/note="Similar to YLR390W-A no_gene_description"
/codon_start=1
/product="Ccw14p"
/protein_id="EWG84592.1"
/translation="MRATTLLSSVVSLALLSKEVLATPPACLLACVAQVGKSSSTCDS
LNQVTCYCEHENSAVKKCLDSICPNNDADAAYSAFKSSCSEQNASLGDSSSSASSSAS
SSSKASSSTKASSSSASSSTKASSSSASSSTKASSSSAAPSSSKASSTESSSSSSSST
KAPSSEESSSTYVSSSKQASSTSEAHSSSAASSTVSQETVSSALPTSTAVISTFSEGS
GNVLEAGKSVFIAAVAAMLI"
gene complement(856096..857652)
/locus_tag="R008_L12881"
mRNA complement(856096..857652)
/locus_tag="R008_L12881"
/product="hypothetical protein"
CDS complement(856096..857652)
/locus_tag="R008_L12881"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84593.1"
/translation="MAPKISISLNPPYNGEFYSSNDQMSGIVSLQLTKALSIRKISVI
LKGFSETLTKIDQEYMFQQNGMMMPGQDNKSFHTLMKFEQRVFPPDNVWNALDGSSKP
FKVKPGSYNYSFQFDKFPRKPECLKNHTAKTVAFVTRNNARLPPTFNSHWQEFNKIDN
LDLYFYSFGKVIYMVQVQIELGKSSSWFKPFHKLIREIETFEFIPEPKDLIVEPDEDD
NEELNAFSNNSRGNSLVTNNEFFNSSNLKVPSKDVKVVNGVGYIKSDRNFSQANSILI
ENGDIRSRPVSSVTSTRQSTRLVNGMKVFPSTYKMGLPDGESNMRIEVRSRDLKQIYR
KDYLFRSGSQNFDKVYVVMEGNIASLSKMQITPLKLQLNLLETTTYLSQGIANGNYSS
LKLIEIDLNQLKSNKPLLDLNEIRENFDGSMFECELRLKDHPILRKLVFNEEDYRHRG
NRLYSFKTCTIKRIFSLQLLIEWGINGIRKQSEVNIDPVQIFCQVREHVEAEALPRYV
PPPTYTEMAS"
gene 858426..859265
/gene="Atp10"
/locus_tag="R008_L12886"
mRNA 858426..859265
/gene="Atp10"
/locus_tag="R008_L12886"
/product="Atp10p"
CDS 858426..859265
/gene="Atp10"
/locus_tag="R008_L12886"
/note="Similar to YLR393W Mitochondrial inner membrane
protein required for assembly of the F0 sector of
mitochondrial F1F0 ATP synthase, interacts genetically
with ATP6"
/codon_start=1
/product="Atp10p"
/protein_id="EWG84594.1"
/translation="MQGTFKRFYHPTLTRMSFLDKFLKPMMATASPKEYQIKQLVKPI
GLTQAPRKSTKYSQGNSLRDMFDSEKTNHRVKELAVEFSKSGLYDVQVFQKTKGKLFI
APVSYWKEDKALFFPHLIGTAMDGTKQQNIEDMLRGKTSIVRLFSTASGDKLSSSYFQ
GIVDDNKKTDYLTEADARLSLNDSNVQIIEVNLVENAVKSALVKTLARWANRVPSWRQ
PFYFECSRGQWPFSVREELFCNNVFSGYVFLVDQQLKIRWAACGEATPSEKEALWKFA
KCL"
gene 859297..860745
/gene="Cst9"
/locus_tag="R008_L12891"
mRNA 859297..860745
/gene="Cst9"
/locus_tag="R008_L12891"
/product="Cst9p"
CDS 859297..860745
/gene="Cst9"
/locus_tag="R008_L12891"
/note="Similar to YLR394W 'SUMO E3 ligase; required for
synaptonemal complex formation; localizes to synapsis
initiation sites on meiotic chromosomes; potential Cdc28p
substrate'"
/codon_start=1
/product="Cst9p"
/protein_id="EWG84595.1"
/translation="MGGYLAIVFIPQTNTKSMREKKQKCLKQVKRLSLISPKKYIMPD
SIFEQPFVYCGVCHRRTSHGDPLRLTSCAHILCSQHSPLTSKVCPICRSSDISILNLV
ESKQLPTDIRIFFEPLPPLLESLYNVSQFQLNGLSKQCQYYQNHCLKLREKCARQQQL
LYQAKIELDSMAILKKRIQELESVLNHNNVSSMSVGVLPTRNSHQNHYQPPPTVDLTV
DDNSLEEFEAKSFIKKLKKNSSLRNSSKNNNGTVTPSTSGRVNKNQPLFMETLNNPNR
DSIPPPGMNPNANSNLPNISTIAESTNLNRFSFSPVRVAKGFDGKLPNLDILTNNGSV
SSKNISRLSSASLQPSSPLSSSSNRLILPNSNLKELHHSNTPLTSTSTQFPSALEKLK
ITRKRNNTISGSNRITHNLSSHVRSSGLAFSSSSNALQQSKLPKSNILKRSNSTQQLT
NTHLKSDNHQPPRSSNTVLGSSKKNNKFRRIR"
gene complement(861076..861312)
/gene="Cox8"
/locus_tag="R008_L12901"
mRNA complement(861076..861312)
/gene="Cox8"
/locus_tag="R008_L12901"
/product="Cox8p"
CDS complement(861076..861312)
/gene="Cox8"
/locus_tag="R008_L12901"
/note="Similar to YLR395C Subunit VIII of cytochrome c
oxidase, which is the terminal member of the mitochondrial
inner membrane electron transport chain"
/codon_start=1
/product="Cox8p"
/protein_id="EWG84596.1"
/translation="MLCQQMIRTTAKRSSNIMTRPIIMKRSVHFKDGVYENIPFKVKG
RKTPYALSHFGFFAIGFAVPFVACYVQLKKSGAF"
gene complement(861582..863657)
/gene="Vps33"
/locus_tag="R008_L12906"
mRNA complement(861582..863657)
/gene="Vps33"
/locus_tag="R008_L12906"
/product="Vps33p"
CDS complement(861582..863657)
/gene="Vps33"
/locus_tag="R008_L12906"
/note="Similar to YLR396C 'ATP-binding protein that is a
subunit of the HOPS complex and of the CORVET tethering
complex; essential for protein sorting, vesicle docking
and fusion at the vacuole'"
/codon_start=1
/product="Vps33p"
/protein_id="EWG84597.1"
/translation="MNRFWNTKKFSLTNADGLCATLNEISQNDEVLVVQPSVLPVLNS
LLTFQDLTQSTPVRKITLLDDQLSDDLPSALGSVPQMDLIFLIDVRTSLRLPPQLLDA
AQKHNLSSLHIIYCRWKPSFQNTLEDTEQWQKDGFDLNSKKTHFPNVIESQLKELSNE
YTLYPWDLLPFPQIDENVLLTHSLYNMENVNMYYPNLRSLQSATESILVDDMVNSLQS
LIFETNSIITNVVSIGNLSKRCSHLLKKRIDEHQTENDLFIKGTLYGERTNCGLEMDL
IILERNTDPITPLLTQLTYAGILDDLYEFNSGIKIKEKDMNFNYKEDKIWNDLKFLNF
GSIGPQLNKLAKELQTQYDTRHKAESVHEIKEFVDSLGSLQQRQAFLKNHTTLSSDVL
KVVETEEYGSFNKILELELEILMGNTLNNDIEDIILELQYQYEVDQKKILRLICLLSL
CKNSLREKDYEYLRTFMIDSCGIGKCFQLESLAELGFFTSKTGKTDLHITTSKSTRLQ
KEYRYISQWFNTVPIEDEHAADKITNENDDFSEATFAYSGVVPLTTRLVQMLYDRSIL
FHNYSSQQPFILSREPRVSQTEDLIEQLYGDSHAIEESIWVPGTITKKINASIKSNNR
RSTDGSNGTFHAAEDIALVVFLGGVTMGEIAIMKHLQKILGKKGINKRFIIIADGLIN
GTRIMNSIS"
gene complement(863896..866238)
/gene="Afg2"
/locus_tag="R008_L12911"
mRNA complement(863896..866238)
/gene="Afg2"
/locus_tag="R008_L12911"
/product="Afg2p"
CDS complement(863896..866238)
/gene="Afg2"
/locus_tag="R008_L12911"
/note="Similar to YLR397C 'ATPase of the CDC48/PAS1/SEC18
(AAA) family, forms a hexameric complex; is essential for
pre-60S maturation and release of several preribosome
maturation factors; may be involved in degradation of
aberrant mRNAs'"
/codon_start=1
/product="Afg2p"
/protein_id="EWG84598.1"
/translation="MAPKSSSSGSKKKSSASSNSADAKASKFKLPAEFITRPHPSKDH
GKETCTAYIHPNVLSSLEINPGSFCTVGKIGENGILVIARAGDEEVHPVNVITLSTTI
RSVGNLILGDRLELKKAQVQPPYATKVTVGSLQGYNILECMEEKVIQKLLDDSGVIMP
GMIFQNLKTKAGDESIDVVITDASDDSLPDVSQLDLNMDDMYGGLDNLFYLSPPFIFR
KGSTHITFSKETQANRKYNLPEPLSYAAVGGLDKEIESLKSAIEIPLHQPTLFSSFGV
SPPRGILLHGPPGTGKTMLLRVVANTSNAHVLTINGPSIVSKYLGETEAALRDIFNEA
RKYQPSIIFIDEIDSIAPNRANDDSGEVESRVVATLLTLMDGMGAAGKVVVIAATNRP
NSVDPALRRPGRFDQEVEIGIPDVDARFDILTKQFSRMSSDRHVLDSEAIKYIASKTH
GYVGADLTALCRESVMKTIQRGLGTDANIDKFSLKVTLKDVESAMVDIRPSAMREIFL
EMPKVYWSDIGGQEELKTKMKEMIQLPLEASETFARLGISAPKGVLLYGPPGCSKTLT
AKALATESGINFLAVKGPEIFNKYVGESERAIREIFRKARSAAPSIIFFDEIDALSPD
RDGSSTSAANHVLTSLLNEIDGVEELKGVVIVAATNRPDEIDAALLRPGRLDRHIYVG
PPDVNARLEILKKCTKKFNTEESGVDLHELADRTEGYSGAEVVLLCQEAGLAAIMEDL
DVAKVELRHFEKAFKGIARGITPEMLSYYEEFALRSGSSS"
gene complement(866498..870361)
/gene="Ski2"
/locus_tag="R008_L12916"
mRNA complement(866498..870361)
/gene="Ski2"
/locus_tag="R008_L12916"
/product="Ski2p"
CDS complement(866498..870361)
/gene="Ski2"
/locus_tag="R008_L12916"
/note="Similar to YLR398C 'Ski complex component and
putative RNA helicase, mediates 3'-5' RNA degradation by
the cytoplasmic exosome; null mutants have superkiller
phenotype of increased viral dsRNAs and are synthetic
lethal with mutations in 5'-3' mRNA decay'"
/codon_start=1
/product="Ski2p"
/protein_id="EWG84599.1"
/translation="MSEGFSSSSIQELYQSLKEITNNADVELFEDRITKLDFESTDEP
KHANDIIKDRFLRPSNALPWSLLDMVQDVPHTSSPEDCSGKLDYKELLKVPDPINRTS
YQFKRTGLEGKISGYKEEVDLKEVANANASNSLSITRSINHNQNSVRGSTAQLPFTPG
GIPMKSVKTGSEQNGSSTMANATKLLHKDGQGLFDIPEGMNRGIKPMDSPAENEDQNG
QFKELKQLNEIDNELDIRIEANEAKLKEEEKSAKSISEEIMEEATEETTADNADDAEI
DELLPIGIDFGRTKPVSKSVPVKKEWAHVVDLNHKIENFDELIPNPARSWPFELDTFQ
KEAVYHLEQGDSVFVAAHTSAGKTVVAEYAIAMAHRNMTKTIYTSPIKALSNQKFRDF
KETFDDVNIGLITGDVQINPDANCLIMTTEILRSMLYRGADLIRDVEFVIFDEVHYVN
DQDRGVVWEEVIIMLPQHVKFILLSATVPNTYEFANWIGRTKQKNIYVISTPKRPVPL
EINIWAKKELIPVINQNSEFLEANFRKHKEILNGESAKGAPSKTDNGRGGSTARGGRG
GSNTRGGRGGRGNSTRGGANRGGSRGAGAIGSNKRKFFTQDGPSKKTWPEIVNYLRKR
ELLPMVVFVFSKKRCEEYADWLEGINFCNNKEKSQIHMFIEKSITRLKKEDRDLPQIL
KTRSLLERGIAVHHGGLLPIVKELIEILFSKGFIKVLFATETFAMGLNLPTRTVIFSS
IRKHDGNGLRELTPGEFTQMAGRAGRRGLDSTGTVIVMAYNSPLSIATFKEVTMGVPT
RLQSQFRLTYNMILNLLRIEALRVEEMIKYSFSENAKETLQPEHEKQIKVLQEELQTI
EYKSCEICDNDIEKFLELMLAYKEATVNLMQEMVKSPSILHILKEGRLVAFRDPNDCL
KLGFVFKVSLKDAVCVIMTFTKPYKLPNGEPNHLIYFPKADGYRRRNFPKFQKTDFYM
EEVPVTAIEVITKRKFAAPLGKVIKKDVAALNEFNAETNNILDGKTLKEAINIEKQGL
KIHQILLDRTNIRDEIFKLKSIKCPNLSQHIVPKFKAHVIKKKIEELYHLMSDQNLSL
LPDYEKRLAVLKDTEFIDQNHNVLLKGRVACEINSGYELVLTELILDNFLGSFEPEEI
VALLSVFVYEGKTREEEPPIVTPRLAKGKQRIEEIYKKMLSVFNTHQIPLTQDEAEFL
DRKRFAMMNVVYEWARGLSFKEIMEMSPEAEGTVVRVITWLDEICREVKTASIIIGNS
TLHMKMSRAQELIKRDIVFAASLYL"
gene complement(870877..872937)
/gene="Bdf1"
/locus_tag="R008_L12921"
mRNA complement(870877..872937)
/gene="Bdf1"
/locus_tag="R008_L12921"
/product="Bdf1p"
CDS complement(870877..872937)
/gene="Bdf1"
/locus_tag="R008_L12921"
/note="Similar to YLR399C 'Protein involved in
transcription initiation at TATA-containing promoters;
associates with the basal transcription factor TFIID;
contains two bromodomains; corresponds to the C-terminal
region of mammalian TAF1; redundant with Bdf2p'"
/codon_start=1
/product="Bdf1p"
/protein_id="EWG84600.1"
/translation="MTDITPVQNDVDVNGNNVNDDVSSNLKRPIDQGDPSNGLAEEEN
PANNQLHVKRARLDGDALTSAPAGLAENGIEGATLAANGENGYNATGSGVEDEQQGLK
KEEGGQGTKQEDLDENSKQELPMEVPKEPAPAPPPEPDMNNLPQNPIPKHQQKHALLA
IKAVKRLKDARPFLQPVDPVKLDIPFYFNYIKRPMDLSTIERKLNVGAYEVPEQITED
FNLMVNNSIKFNGPNAGISQMARNIQASFEKHMLNMPAKDAPPVIAKGRRSSAQEDTP
IVIRRAQTHNGRPKRTIHPPKSKDIYPYESKKPKSKRLQQAMKFCQSVLKELMAKKHA
SYNYPFLEPVDPVSMNLPTYFDYVKEPMDLGTIAKKLNDWQYQTMEDFERDVRLVFKN
CYTFNPDGTIVNMMGHRLEEVFNSKWADRPNLDDYDSDEDSRTQGDYDDYESEYSESD
IDETIITNPAIQYLEEQLARMKVELQQLKKQELEKIRKERRLARGSKKRGKRSKGRSG
SKNASSKGRRDKKNKLKTVVTYDMKRIITERINDLPTSKLERAIDIIKKSMPNISEDD
EVELDLDTLDNHTILTLYNTFFRQYESSSGASNGLDGTSGVTRDASSLSPTSAGSRKR
RSKALSQEEQSRQIEKIKNKLAILDNASPLSQNGSPGQIQSAAHNGFSSSSDDDVSSE
SEEE"
gene complement(873782..875788)
/gene="Dus3"
/locus_tag="R008_L12941"
mRNA complement(873782..875788)
/gene="Dus3"
/locus_tag="R008_L12941"
/product="Dus3p"
CDS complement(873782..875788)
/gene="Dus3"
/locus_tag="R008_L12941"
/note="Similar to YLR401C 'Dihydrouridine synthase, member
of a widespread family of conserved proteins including
Smm1p, Dus1p, and Dus4p; contains a consensus oleate
response element (ORE) in its promoter region'"
/codon_start=1
/product="Dus3p"
/protein_id="EWG84601.1"
/translation="MEQNAEKRSIVGDDNSTVKRQDTSPSKGIAHIKPEYIVPLKQNE
NQKVAIYDEEMSSDRMTNEFAGGTNKKNKNGRGKKRGQNKNRDNRQVKEQNVLCPRLI
HGDISKCSFGDNCRFVHDINLYLSTKKPEVESNIFPSCPVFNSLGFCPMGFKCRFLSS
HLNKEDNILISKKEIDPDAQTIWSVKGEVNHISPERKLDLIKRRFPFTKSNEILEIID
SFQQECRDSMKPEEEVESTPQLKKQDPDVEQPVAPQVEQRNKELSQHRMKQREVYLKY
KDTRYFAQEKKPLDLYHKKIVSPLTTVGNLPYRRLMRKLGADVTYSEMALAVPLIQGT
NSEWALPKAHTSEFPGFGVQVACSKAWQAAKAAEALANSVSEISEINLNSGCPIDLLY
RQGSGSALLDNPARMIRCLNAMNYVSKDIPITVKIRTGTKEGHPIAEGLVKRLVNETD
VAAITLHGRSRQQRYTKSADWDYVSQVADTLRSAEADFIETEQGKEGRDSKNRIQFVG
NGDVNNFEDWYRYLDGNENIDSVMVARGALIKPWIFEEVESQQYLDKTSTERLDILRD
YAQFSMEHWGTDEYGISQCRRFFCEFMSFFHRYVPMGICERYPVKLNERPPNWCGRDE
LETLMGSTDVNDWIKLSDLFFGKTDENFVFVPKHKSSSYANRDS"
gene 876907..878958
/gene="Sfp1"
/locus_tag="R008_L12956"
mRNA 876907..878958
/gene="Sfp1"
/locus_tag="R008_L12956"
/product="Sfp1p"
CDS 876907..878958
/gene="Sfp1"
/locus_tag="R008_L12956"
/note="Similar to YLR403W 'Transcription factor that
controls expression of ribosome biogenesis genes in
response to nutrients and stress, regulates G2/M
transitions during mitotic cell cycle and DNA-damage
response, modulates cell size; regulated by TORC1 and
Mrs6p'"
/codon_start=1
/product="Sfp1p"
/protein_id="EWG84602.1"
/translation="MDFTTMTMASNMATSTTTTATSAHASINSSSNSNIDIDSNQNTP
SILINNNSDSSNGKNTDFNGVNNIHQKNITNNTNNVHLYSPNIMDQTLLTPQDIAKLR
RESIAHSQGMGGVSWGSISVGSWLRDEIISRRSSIVPASANGAASAAASATTTATNTL
QIQQPTKRPSVSNPPYHRGYSISPQIAYTAYLPNLEKQYCKDYSCCGLSLPGLHDLLR
HYEEAHISTSPNTTNMSQIPMNSAGNTSSSVRMTNNTSSANYNLQNNMAANTKNAGHK
TNTMQAHSGNATNNTAINNMHANLQSNMDSNSTIRQSQHPHHQQNIIQQQLQSNSVNH
TSGAVPTPSVMGSATASSTTANPNVISITGAPNSGLSMANHSQQLHLNGNLVDAVSTN
DVFLRTSNSPSRHVPHNKQINSNSNSGININNNTSHNSNINMGSKNAMVNRPHTFNNY
SLNKTSRNPIQHQSRKIDPHQTDLSPLVLVQDIDLSFMDDDILGPSNHNSMNSVVNPT
TGSHNYNTFHSSVHAKSSQNMVEDQDIDDIDDDDDVDDDDDDDDDDDTENGSSSNGKS
VHNNNYKMPQQAYIDDPARRLYVMDHEEQKPFKCPVIGCEKTYKNQNGLKYHRLHGHQ
NQKLHENPDGTFSVIDPDSTDSFGDGMGSAKDKPYRCEVCGKRYKNLNGLKYHRGHST
H"
gene 880083..880940
/gene="Sei1"
/locus_tag="R008_L12966"
mRNA 880083..880940
/gene="Sei1"
/locus_tag="R008_L12966"
/product="Sei1p"
CDS 880083..880940
/gene="Sei1"
/locus_tag="R008_L12966"
/note="Similar to YLR404W 'Seipin protein involved in
lipid droplet morphology, number, and size; proposed to be
involved in lipid metabolism; related to the human BSCL2
which is associated with lipodystrophy'"
/codon_start=1
/product="Sei1p"
/protein_id="EWG84603.1"
/translation="MKINVSRPLQFLQWSSYIVVAFLIQLLIILPLSILIYHDFYLRL
LPADSSNVVPLNTFNILNGVQFGTKFFQSIKSIPVGTDLPQTIDNGLSQLIPMRDNME
YKLDLNLQLYCQSKTDHLNLDNLLIDVYRGPGPLLGAPGGSNSKDEKIFHTSRPIVCL
ALTDSMSPQEIEQLGPSRLDVYDEEWLNTIRIEDKISLESSYETISVFLKTEIAQRNL
IIHPESGIKFRMNFEQGLRNLMLRKRFLSYIIGISIFHCIICVLFFITGCTAFIFVRK
GQEKSKKHS"
gene 881129..882232
/gene="Dus4"
/locus_tag="R008_L12971"
mRNA 881129..882232
/gene="Dus4"
/locus_tag="R008_L12971"
/product="Dus4p"
CDS 881129..882232
/gene="Dus4"
/locus_tag="R008_L12971"
/note="Similar to YLR405W Dihydrouridine synthase, member
of a widespread family of conserved proteins including
Smm1p, Dus1p, and Dus3p"
/codon_start=1
/product="Dus4p"
/protein_id="EWG84604.1"
/translation="MHTMHIPSGDVLIPKPKLITEETDPLHIIKTRQKTHGRPVTIAG
PMVRYSKLPFRQLCREYNVDIVYSPMILAREYVRNEHARISDLSTNNEDTPLIVQVGV
NNVADLLKFVEMVAPYCDGIGINCGCPIKEQIREGIGCALIYNSDLLCSMVHAVKDKY
GDKLRIETKIRIHEALDETVELCRKLCDAGVDWITIHGRTRRTRSSQPANLDAIKYII
ENISDKNVPVIANGDCFKLSDLERITKYTGAHGVMAVRGLLSNPALFAGYTTCPWGCI
EKFCYWALEFGGLPFQLAQHHLYCMLENMELKKSLLKEMMNLKNYISLIDWFNKTFEF
KRYGEDGFGMGVEIPYKANSCVQRSASVVERQE"
gene complement(882403..883093)
/gene="Rpl31b"
/locus_tag="R008_L12976"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(883548..883694)
/locus_tag="R008_L12986"
mRNA complement(883548..883694)
/locus_tag="R008_L12986"
/product="hypothetical protein"
CDS complement(883548..883694)
/locus_tag="R008_L12986"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84605.1"
/translation="MIQKPILLSSFLFLYIRALLHSIHPYIRTSVHLYTKKNHLIQFF
RSAV"
gene 884306..884992
/locus_tag="R008_L12991"
mRNA 884306..884992
/locus_tag="R008_L12991"
/product="hypothetical protein"
CDS 884306..884992
/locus_tag="R008_L12991"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84606.1"
/translation="MTVSTSKTPKKNIKYTLTHTLQKWKETLKKITHETLSSIDDSSG
SDEKIEALFTVSQPAVVASKGIDRDSGASMSQVGGGVNSTLEMKLTDESEESSSANNT
TTTASHTLSNSKKSTQNFENYNVVEERIKLAQKSKAPFCNAEKIWKRRRQLWTQPTEQ
SESANNDGVTRREIFQAIPQEYYARVYKKLVVDDKPLREPLNLEDALQVINAGWTETR
KWANAAKGMP"
gene complement(885215..885592)
/locus_tag="R008_L12996"
mRNA complement(885215..885592)
/locus_tag="R008_L12996"
/product="hypothetical protein"
CDS complement(885215..885592)
/locus_tag="R008_L12996"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84607.1"
/translation="MFLTFSMCVNWIIVKMPNRSEELDRLLDKIINSPHRTEASKTLQ
EIENNQSYILNVQLKKLLRLHDDSFKNKCVSPINYMLEKYTPYMGHTEALQKEAELVD
RDLRILEMDVPTDRKESQLEIKR"
gene complement(885755..888574)
/gene="Utp21"
/locus_tag="R008_L13001"
mRNA complement(885755..888574)
/gene="Utp21"
/locus_tag="R008_L13001"
/product="Utp21p"
CDS complement(885755..888574)
/gene="Utp21"
/locus_tag="R008_L13001"
/note="Similar to YLR409C 'Subunit of U3-containing 90S
preribosome and Small Subunit (SSU) processome complexes
involved in production of 18S rRNA and assembly of small
ribosomal subunit; synthetic defect with STI1 Hsp90
cochaperone; human homolog linked to glaucoma'"
/codon_start=1
/product="Utp21p"
/protein_id="EWG84608.1"
/translation="MSIDLKKRKVEEDVRSRGKNSKIFSPFRIIGNVSNGVPFATGTL
GSTFYIVTCVGKTFQIYDANTLHLLFVSEKETPSSIVALSAHFHYVYAAYENKVGIYK
RGIEEHLLELETDANVEHLCIFGDYLCASTDDNSIFIYKKSDPQDKYPSEFYTKLTVT
EIQGGEIVSLQHLATYLNKLTVVTKSNVLLFNVRTGKLVFTSNEFPDQITTAEPAPVL
DIIALGTVTGEVIMFNMRKGKRIRTIKIPQSRISSLSFRTDGSSHLSVGTSSGDLIFY
DLDRRSRIHVLKNIHRESYGGVTQATFLNGQPIIVTSGGDNSLKEYVFDPSLSQGSGD
VVVQPPRYLRSRGGHSQPPSYIAFADSQSHFMLSASKDRSLWSFSLRKDAQSQEMSQR
LHKKQDGGRVGGSTIKSKFPEIVALAIENARIGEWENIITAHKDEKFARTWDMRNKRV
GRWTFDTTDDGFVKSVAMSQCGNFGFIGSSNGSITIYNMQSGILRKKYKLHKRAVTGI
SLDGMNRKMVSCGLDGIVGFYDFNKSTLLGKLKLDAPITAMVYHRSSDLFALALDDLS
IVVIDALTQRVVRQLWGHSNRITAFDFSPEGRWIVSASLDSTIRTWDLPTGGCIDGII
VDNVATNVKFSPNGDLLATTHVTGNGICIWTNRAQFKTVSTRTIDESEFARMALPSTS
VRGNDSMLSGALESNGGEDLNDIDFNTYTSLEQIDKELLTLSIGPRSKMNTLLHLDVI
RKRSKPKEAPKKSEKLPFFLQLSGEKVGDEASVREGIAHETPEEIHRRDQEAQKKLDA
EEQMNKFKVTGRLGFESHFTKQLREGSQSKDYSSLLATLINFSPAAVDLEIRSLNSFE
PFDEIVWFIDALTQGLKSNKNFELYETFMSLLFKAHGDVIHANNKNQDIASALQNWED
VHKKEDRLDDLVKFCMGVATFVTTA"
gene 888882..892322
/gene="Vip1"
/locus_tag="R008_L13006"
mRNA 888882..892322
/gene="Vip1"
/locus_tag="R008_L13006"
/product="Vip1p"
CDS 888882..892322
/gene="Vip1"
/locus_tag="R008_L13006"
/note="Similar to YLR410W 'Inositol hexakisphosphate (IP6)
and inositol heptakisphosphate (IP7) kinase; IP7
production is important for phosphate signaling; involved
in cortical actin cytoskeleton function, and invasive
pseudohyphal growth analogous to S. pombe asp1'"
/codon_start=1
/product="Vip1p"
/protein_id="EWG84609.1"
/translation="MSGIKKEPIESDEVPQQETKNNLPSAPSEMSPLFLNKNTQKAMQ
SIAPILEGFSPKTSASENMSLKLPPPGIQDDHSEENLTVHDTLQRTISTALGNGNNTN
TVTTSGLKKADSESKSEADPEGLSNSNIVNDADNINSISKTGSPHLPQGTMDAEQTNM
GTNSVPTSSASSRKSSTSHPKPRLPKVGKIGVCAMDAKVLSKPMRHILNRLIEHGEFE
TVIFGDKVILDERIENWPTCDFLISFFSSGFPLDKAIKYVKLRKPFIINDLIMQKILW
DRRLCLQVLEAYNVPTPPRLEISRDGGPRANEELRAKLREHGVEVKPVEEPEWKMVDD
DTLEVDGKTMTKPFVEKPVDGEDHNIYIYYHSKNGGGGRRLFRKVGNKSSEFDPTLVH
PRTEGSYIYEQFMDTDNFEDVKAYTIGENFCHAETRKSPVVDGIVRRNTHGKEVRYIT
ELSDEEKTIAGKVSKAFSQMICGFDLLRVSGKSYVIDVNGFSFVKDNKAYYDSCANIL
RSTFIEAKKKMDMEKKNLPIIREEKEQKWVFKGLAIIIRHADRTPKQKFKHSFTSPIF
ISLLKGHKEEVVIRNVNDLKIVLQALRIALDEKAGNPAKIKVLANALEKKLNFPGTKI
QLKPVLNKENEVEKVQFILKWGGEPTHSAKYQATELGEQMRQDFDLLNKSILQNIKIF
SSSERRVLHTAQYWTRALFGADELGSDEISIRKDLLDDSNAAKDLMDKVKKKLKPLLR
EGKEAPPQFAWPSKMPEPYLVIKRVVELMNYHKKIMDNNFAKKDVNSMQTRWCTSEDP
SLFKERWDKLFKEFNNAEKVDPSKISELYDTMKYDALHNRQFLENIFDPGLPNEAIAD
ELGSHSLVDRYPINVLAKNNFKIIDSHSMNNSGKNSSNSVGSLGWVLESGKTSTARNP
KSSSQFDEPRFMQLRELYKLAKVLFDFICPKEYGISDAEKLDIGLLTSLPLAKQILND
IGDMKNRETPACVAYFTKESHIYTLLNIIYESGIPMRIARNALPELDYLSQITFELYE
STDASGQKSHSIRLKMSPGCHTQDPLDVQLDDRHYISCIPKISLTKHLDMDYVQQKLR
NKFTRVIMPPKFTPVNITSPNLSFQKRKTRRKSVSVEKLKLPASSGSSSSTSVNKTLD
"
gene 892629..893354
/gene="Ctr3"
/locus_tag="R008_L13016"
mRNA 892629..893354
/gene="Ctr3"
/locus_tag="R008_L13016"
/product="Ctr3p"
CDS 892629..893354
/gene="Ctr3"
/locus_tag="R008_L13016"
/note="Similar to YLR411W 'High-affinity copper
transporter of the plasma membrane, acts as a trimer; gene
is disrupted by a Ty2 transposon insertion in many
laboratory strains of S. cerevisiae'"
/codon_start=1
/product="Ctr3p"
/protein_id="EWG84610.1"
/translation="MNMGGSSSTAAKKATCKISMLWNWYTIDTCFIARSWRNDTKGKF
AGSCIGCFALVVVAQWLTRFSRQFDVELLKRQKIKHLASYSPEEYVVKCGDEDAKSDI
EELQGFYNEPSWKTTLISLQKSFIYSFYVWGPRRLNEPEDDLLKKVLSCCTLITPVDL
YPTFLDHMIRVTIFVLQWGLSYIIMLLFMYYNGYIIISCLIGAIVGRFIFCYEPLGSL
GANGSAQGTVSYDKESDDRKCCL"
gene 893744..894568
/locus_tag="R008_L13021"
mRNA 893744..894568
/locus_tag="R008_L13021"
/product="hypothetical protein"
CDS 893744..894568
/locus_tag="R008_L13021"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84611.1"
/translation="MDFKASGTKFKKTGKKVVPAKAFEEIVQTNRDVLKKSAFLSDLL
ENLQPHLNNIKKIRCVAIGNFKEDFPATYQFALLLEIIDYIKSEDERDVVVSLYDPIF
TKEEIQYLKSLGSKWLIEEEFSENDAIDYESVLYFLPHAPLDLTENILSSQRPHLWLA
NNMISHTDRYTKAKLCENYPNLSKLVHYLQTNAPPEVKKAHDVDGFATFIPKRKRKNR
NNSSKLKVTPSDIDYDSIAPKFKSCQILTDFDEGKYLKEKPWINSFSDLTLHAIEY"
gene complement(895663..895848)
/locus_tag="R008_L13036"
mRNA complement(895663..895848)
/locus_tag="R008_L13036"
/product="hypothetical protein"
CDS complement(895663..895848)
/locus_tag="R008_L13036"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84612.1"
/translation="MHLCQNGHYYKPHRASAEKVPYLKKKKKNSRNGGKAKKRMNRKK
KGTVEFSQQKKRKKTGT"
gene 896531..898558
/locus_tag="R008_L13041"
mRNA 896531..898558
/locus_tag="R008_L13041"
/product="hypothetical protein"
CDS 896531..898558
/locus_tag="R008_L13041"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84613.1"
/translation="MNFKILLPICALLTLTTFLLTIIATAGSTSNYKPITNIYIGDAD
ISKINVTKVMPQVGPILTVLGSALTAPNTTVDTIFGALKAIASTEALSPLLHLLSNAA
NTSATLSSLTQLAPMALLGTNTATTTAFSALDELLTTSKNTTELLDGFSTLMSSMSTN
TSSTSASLENTVLTLLVDSTNPIGTTESLITLNNMTTEEKTKLSPVFELFASSKNITA
TCDALETIMNSTIPTSTVSSLFSSLKTSLAEGGNATETIMQLGSLVPSSLKPAVQAVV
TLFDETTSQNVTLSVLSTMIAENITQSSSAKAAMGALTDLLNYTTNQTELLTSVESLA
LSKEAASSTNQLVALDEILSASANASTVVSIIPTLESQLANNTVLLKYVPYLFSLLAA
SSDPVSSFSSLVNITKWAETNAATFMPMLKILNSAVNMTTITPEQLKEMTPSILEYLH
IPVIYRLSIFTMCRAHLNRTMYSCSKSHAVQNMDFRSIVYNNIEGSDFKPYMDALNIG
KDDLHLDGELQDRQHMYVPAVKAALAMNLMCIITSFFLMVFLLLLSRRSVVSQKLWLA
LGFISCWICIFSGLGSTIFSVILNMMKSGSKKDNYDVIISGSSPFYGLMWSGFVFAVL
VFLCIAYCWWISRKGAAIVEAEKAVQESDSTTSRIIEEHESPIDAEKNFAR"
gene complement(898728..899519)
/locus_tag="R008_L13051"
mRNA complement(898728..899519)
/locus_tag="R008_L13051"
/product="hypothetical protein"
CDS complement(898728..899519)
/locus_tag="R008_L13051"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84614.1"
/translation="MRNFFTLFFAAIFSLGALILAIVACAGSTKNYSPINKIYCAELD
LSQMKVSTVLPSLSSATLSSLGLPSYINIGLWSYCTVDSSHNIQSCSSPHGIQNFNLS
SLVYDNINNNEALELMDSVASVVLPEKLKSKMTYYNNLVKCMFITILIGIVLTFVNLV
FNVLRWIIHIRPLTWFGAFFSFFAFAALLVSIGSCLGTYSYIKYILKHNYSDYGISMS
IGRNYQGLMWGAVVGALLNFILWCSVRSRPTVIYANAPIEEKPLI"
gene complement(899634..899972)
/locus_tag="R008_L13061"
mRNA complement(899634..899972)
/locus_tag="R008_L13061"
/product="hypothetical protein"
CDS complement(899634..899972)
/locus_tag="R008_L13061"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84615.1"
/translation="MYLSAQLMRTVTASHLTLRALSTPPLFQHRQIAAVEWCGTTRPG
LAWQKRTQHASSVISKSGVLSAKPSSVFLALLAGKLAEKYIYARMLLFHVSVVNECPV
VFHSGPVVWK"
gene 900385..902085
/gene="Vps36"
/locus_tag="R008_L13071"
mRNA 900385..902085
/gene="Vps36"
/locus_tag="R008_L13071"
/product="Vps36p"
CDS 900385..902085
/gene="Vps36"
/locus_tag="R008_L13071"
/note="Similar to YLR417W 'Component of the ESCRT-II
complex; contains the GLUE (GRAM Like Ubiquitin binding in
EAP45) domain which is involved in interactions with
ESCRT-I and ubiquitin-dependent sorting of proteins into
the endosome'"
/codon_start=1
/product="Vps36p"
/protein_id="EWG84616.1"
/translation="MEYWHYVETTSSGQPLLREGEKDIFIDQSVGLYHGKSKILQRQR
GRIFLTSQRIIYIDDAKPTQNSLGLELDDLAYVNYSSGFLTRSPRLILFFKDPSSKDE
LGKSAETASADVVSTWVCPICMVSNETQGEFTKDTLPTPICINCGVPADYELTKSSIN
CSNAIDPNANPQNQFGVNSENICPACTFANHPQIGNCEICGHRLPNASKVRSKLNRLN
FHDSRVHIELEKNSLARNKSSHSALSSSSSTGSSTEFVQLSFRKSDGVLFSQATERAL
ENILTEKNKHIFNQNVVSVNGVDMRKGASSHEYNNEVPFIETKLSRIGISSLEKSREN
QLLNNDILFNNALTDLNKLMSLATSIERLYKNSNITMKTKTLNLQDESTVNEPKTRRP
LLILDREKFLNKELFLDEIAREIYEFTLSEFKDLNSDTNYMIITLVDLYAMYNKSMRI
GTGLISPMEMREACERFEHLGLNELKLVKVNKRILCVTSEKFDVVKEKLVDLIGDNPG
SDLLRLTQILSSNNSKSNWTLGILMEVLQNCVDEGDLLIDKQLSGIYHYKNSYWPSHI
"
gene complement(902289..903470)
/gene="Cdc73"
/locus_tag="R008_L13076"
mRNA complement(902289..903470)
/gene="Cdc73"
/locus_tag="R008_L13076"
/product="Cdc73p"
CDS complement(902289..903470)
/gene="Cdc73"
/locus_tag="R008_L13076"
/note="Similar to YLR418C 'Component of the Paf1p complex
that binds to and modulates the activity of RNA
polymerases I and II; required for expression of certain
genes, modification of some histones, and telomere
maintenance'"
/codon_start=1
/product="Cdc73p"
/protein_id="EWG84617.1"
/translation="MANSLDRLREHLKNGDKLVLKNNEGQSTDDITKATMVETLSSDG
STQDSFPLNEETEIEIDGSLVQLRIIVHCWMNKDSSAADYLADCQNKQLTNVSFLQRT
DLINWLSGNTESSQYLKAPGQKGETSDKVDIENKTLAGELSTVKSTTSASLENDSEVS
DPVVVETMKHERILVDHNSALRGAKPINFGYLIKDTELKLVQSIKGSLRGSKLPPGHK
GAHGRISKTNGSSGGPRKDPIILIPSAASSILTVANIKQFLLESKYVNPRNLPSVPNG
LVNIEKNFERISRPIRFIIVDNTRMFTKPEYWDRVVAIFTTGHTWQFNNYQWNSPQEL
FQRCKGYYFHFAGDSVPQHVQQWNVEKVELDKNKRFKDVEVVRYFWHSLEKELISRGY
R"
gene 903803..908108
/locus_tag="R008_L13081"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(908345..908428)
/locus_tag="R008_L13086"
tRNA complement(908345..908428)
/locus_tag="R008_L13086"
/product="tRNA-Leu"
/note="TL(UAA)L"
gene 909159..910253
/gene="Ura4"
/locus_tag="R008_L13091"
mRNA 909159..910253
/gene="Ura4"
/locus_tag="R008_L13091"
/product="Ura4p"
CDS 909159..910253
/gene="Ura4"
/locus_tag="R008_L13091"
/note="Similar to YLR420W Dihydroorotase, catalyzes the
third enzymatic step in the de novo biosynthesis of
pyrimidines, converting carbamoyl-L-aspartate into
dihydroorotate"
/codon_start=1
/product="Ura4p"
/protein_id="EWG84618.1"
/translation="MVQEIDLDLTCDMHVHVREGAMCELVTPKIRDGGVSIAYIMPNL
QPPITTLDRVIEYKKTLQKLAPKTTFLMSFYLSKDLTPDLIHEAAQQHAIRGVKCYPA
GVTTNSAAGVDPNDFSAFYPIFKAMQEENLVLNLHGEKPSVHDGDKEPIHVLNAEEAF
LPALKKLHNDFPNLKIILEHCTSESAIKTIEDINKNVKKATDVKVAATLTAHHLFLTI
DDWAGNPVNFCKPVAKLPNDKRALVKAAVSGKPYFFFGSDSAPHPVQNKAKYEGVCAG
VYSQSFAIPYIAQVFEEQNALENLKGFVSDFGISFYEVKDSEVASSDKAILFKKEQVI
PQVISDGKDISIIPFKAGDKLSWSVRWEPR"
gene complement(910464..910934)
/gene="Rpn13"
/locus_tag="R008_L13096"
mRNA complement(910464..910934)
/gene="Rpn13"
/locus_tag="R008_L13096"
/product="Rpn13p"
CDS complement(910464..910934)
/gene="Rpn13"
/locus_tag="R008_L13096"
/note="Similar to YLR421C 'Subunit of the 19S regulatory
particle of the 26S proteasome lid; acts as a ubiquitin
receptor for the proteasome; null mutants accumulate
ubiquitinated Gcn4p and display decreased 26S proteasome
stability'"
/codon_start=1
/product="Rpn13p"
/protein_id="EWG84619.1"
/translation="MSMSSTVIKFRAGVCEYNEDSRLCTPIPVQGEIEIKPNEEEELG
FWDFEWRPTEKPVGRELDPISLILIPGETMWVPIKSSKSGRIFALVFSSNERYFFWLQ
EKNSGNLSLNELSTKDKEIYNKMIGVLNNSSESDEEESNDEKQKAQDVDVSMQD"
gene 911271..917069
/locus_tag="R008_L13101"
mRNA 911271..917069
/locus_tag="R008_L13101"
/product="hypothetical protein"
CDS 911271..917069
/locus_tag="R008_L13101"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84620.1"
/translation="MSQQDSQRWLPTDRLIYGVLVKSFLPLQRYPELVYENSDYANVY
VGAEVYVFEESVDKKWCRAYQCLRPFPEEFISNMNSANDVLPDVKPKVVIFPRKYVHF
EAEKAVSTMPFFKAPSAEDFKPLISKECESRSFCDSLYVSSTDDISTGKPRKTPRPPF
PFFRYQKRSFKDEMGPILSLISSHVYSMYSIGEFSIYRKMIKLYYDLDTIRFRLSMNL
TTEAEKINLIRAATSLRTKIAKFLSSTYRKNKLIANSTPRNPDPYGFEGIFARDIDTG
ELLSYEIDKLRTLVSSSMLCGLTNNFPTVPVVESDDESSSNGLFGTVRSSILVNLKDL
AWDPSISDPKYQDLSICVYLRTKDEVLTESFTMTKSSNMESALDEIPAMLFKNILETI
VHKNKVYLVVVLKETIAITTETAPEISSYNISTEESSSHSPFSPFNSSTENKIDHVKK
GLAAGVIDISPVFKFYNGPSVANKAQRFNLYLYSSDSSDSQNFNSSKDADLGWGGLIN
KIIKDSSEGVSVNPRAVSLSVTVKEIIGKQEAEKVLSTSLVPIRSIPTYFYDTMFSQA
ERIYLNLGRVSLYGLPAADTNIENVTVQISCRNKAVKFCKNKLEERSGDWKFVSVRPN
ESIGESIRIEGVENMNEDETLRVLVYLNGFLMAKSNIHIKKKNEIIEYRKGTVFQIMS
SKSVPLIHLELEASYFGRRYNINPAITNFLVLQTKNVEFDQQLKEHYSVTLKQLNNVS
FKDLLKHFDTILAHYLLLLESVNEATDKKGPSSSLPNIVFSEFVKFLNLMLTHQENSR
YWFNRLYKKVMSKELECPNVAPILIKHMTTIFDRSHSSWTRTGTAICRTILYIIVLAI
GSSHSDEMPNFSHFFRSLHKFLMLADEPIMADQILLIESIPSMLETMTNHCKVEDLVR
FAIGLFERCQEKEMNQKMYSRLLSVREEEYLNTKFNCLLKLINKKVLQNYLTNTESVD
KLRLQFLSKTLEWLLTPYTPGDDKCFHVESLRLVNSVFITIIEDYKFDMLQRNLIRLL
PYLCKSFVHLRRYCKKARLMRPRRVFTMLFPREIPCNYIPVDSIVNDEVVVEVLLELA
IIICEITKIASSRFPSYQSFSEIINLCDKDTLFQSNFYSRQITNENVYTITKTVFLFF
KQDWFPGMKWLGVSALLGRSSLILLSLCKDYIIENNSPSPSKESEKRVDMRLWAEYVK
VILLVSNHKSASLTKLAITPRKAVYLISGDLKKISAYILNECWDALATGHYNITYAKK
YGLGALSDCQFELFVHNQFLIREIFIFAFHRHIDATRICCKILWGLGLNFWRIFGSLQ
PAVNACIPELFSAYQIGKLRLNDYELERFVSCLFFMMHVPDSDTFFPACMDFLRDLLG
FLHIVNEIYKIPNQEEFDDDRTARHIEMFEYLLEANRPELFHKMIYDLFIHFIQKKDF
VQAALSLELLAGTYAWDSNDTLEAISFPPLPEQSSFERKEYLLKESARNFSRGQKPEK
ALAVYKDLIKAYDEINYDLNGLAFVHDQIAGIYTRLQSIDRLVPTYFKVSFMGFGFPK
SLRNKSFVFEGLPFEHITSMHDRLLRSYHGSNIVHSQEEVDMLLMNPPMGKYIHVASV
EPCLSISDNYNSSDKKSSINNKVRMYIENRDLRTFSNSRRLPGAKGVTDLWVEEYTYH
TMNTFPTLMNRSEIVKVTKSKLSPLENAIRSLQVKIQELYGLENMCNKTLKDHGDVND
LFTELSTNITGTISAPVNGGISQYKAFLEPSTSKQFSTDDLGRLTLAFDELVAVLGRC
LTLHAELLPSKDLKPSHDLLVRLFEENFAEEIERYSRTLSEGNRSRNNMITARIISHK
NPNKKASFSGRDHHTSGSNHSQFVLEHSDSFGPNSLLFGKYLTRTLSHSSTTSSLDKS
GIVSGTSSTFLAGSQPNTNTDSQHKHDYSHSG"
gene complement(917291..918544)
/gene="Atg17"
/locus_tag="R008_L13106"
mRNA complement(917291..918544)
/gene="Atg17"
/locus_tag="R008_L13106"
/product="Atg17p"
CDS complement(917291..918544)
/gene="Atg17"
/locus_tag="R008_L13106"
/note="Similar to YLR423C 'Scaffold protein responsible
for phagophore assembly site organization; regulatory
subunit of an autophagy-specific complex that includes
Atg1p and Atg13p; stimulates Atg1p kinase activity'"
/codon_start=1
/product="Atg17p"
/protein_id="EWG84621.1"
/translation="MNEADVTKFVNNARKTLTDAQLLCSSANLRIVDIKKKLSSWQLS
ISKLNFLIVGLRQQGKFLYTILKEGIGTKLIQKQWNQAVLVVLVDEMKYWQYEITSKV
QRLDGIVNELSISEKDDTDPSKLGDYISRDNVNLLNDKLKEVPVIERQIENIKLQYEN
MVRKVNKELIDTKLTDVTQKFQSKFGIDNLMETNVAEQFSRELTDLEKDLAEIMNSLT
QHFDKTLLLQDKKIDNDEREELFKVVQGDDKELYNIFKTLHEVIDDVDKTILNLGQFL
QAKIKEKTELHSEVSEIINDFNRNLEYLLIFKDISNLIDSFKNSCTQDIQTTKELCEF
YDNFEESYGNLVLEAKRRKDVANRMKTILKDCEKQLQNLDAQDQEERQNFIAENGTYL
PETIWPGKIDDFSSLYILNYNVKNP"
gene 918769..920895
/gene="Spp382"
/locus_tag="R008_L13111"
mRNA 918769..920895
/gene="Spp382"
/locus_tag="R008_L13111"
/product="Spp382p"
CDS 918769..920895
/gene="Spp382"
/locus_tag="R008_L13111"
/note="Similar to YLR424W 'Essential protein that forms a
dimer with Ntr2p; also forms a trimer, with Ntr2p and
Prp43p, that is involved in spliceosome disassembly; found
also in a multisubunit complex with the splicing factor
Clf1p; suppressor of prp38-1 mutation'"
/codon_start=1
/product="Spp382p"
/protein_id="EWG84622.1"
/translation="MEDSDSNTDKKFFFKKRRIDSYNYSDEEDNNSSMNSDMTYTNDA
LKTSSGNAPTISKLTKTYGIGAKLLSSMGYVAGKGLGKDGSGITTPIETQSRPMHNAG
LGMFSNTNSSNYHSENEDYLSSEDEVVEGIEQVKFNKTSTEVLGEALLNDSGDMTIVR
TLRELRLAGVQLPESILKELDPLNAVPKPKKDVVVEILQELLGIEKSLEAIRQRTSPL
EVQVKEYYGQERLLSELEVTLRDESKHVSLYDKIGAILKLSDDELIDRLTSCLLRKEL
LIEFDLDHLEKPNDILDELTQIIELLAYRMDTTSKFLNRTQTTIFKVIYPKLKKFWEG
FDMTKSKIDSAITLLLDFQQVLSFIGCKEHIMEEFVYPKLLQELDNWELHDEVDHVSP
RIWVLDFMVLIDDKIKDTIVDKIEAKFFAYCKNWYHRESFCITNSDIIFIKELICERR
YYKILCKEFLPKFLDELWERHNDPIYELEDWKEKQEWKEKDSGFFYFMKKLRSYTHYF
HPKQYELMMRGTFNNINKILYQWHLYSTVEDLHKSKWWLNWLMNTVFEHSLPTEIELS
EIRKSYNIFAMSHRYHLDKSTLDEDFDLRQGLRNLMETQVIDDISQSEQEPTYTVQNI
PLGKVSSSFKDVVEDYCLEKGYLISKIPNRYTQLPYGRDQDCIVPLFEIRNGKKKMEV
ALKHDILWVEDSSGTFKPIYLWALDL"
gene complement(921357..921430)
/locus_tag="R008_L13116"
tRNA complement(921357..921430)
/locus_tag="R008_L13116"
/product="tRNA-Asn"
/note="TN(GUU)L"
gene 922965..926888
/gene="Tus1"
/locus_tag="R008_L13126"
mRNA 922965..926888
/gene="Tus1"
/locus_tag="R008_L13126"
/product="Tus1p"
CDS 922965..926888
/gene="Tus1"
/locus_tag="R008_L13126"
/note="Similar to YLR425W 'Guanine nucleotide exchange
factor (GEF) that functions to modulate Rho1p activity as
part of the cell integrity signaling pathway; multicopy
suppressor of tor2 mutation and ypk1 ypk2 double mutation;
potential Cdc28p substrate'"
/codon_start=1
/product="Tus1p"
/protein_id="EWG84623.1"
/translation="MYRYNRSSPFERTPEKRVSRQESQRKSIELPKLPPLNTRNSFLD
DSDNGTDNISIGWTPISDTQQFQSPVPQAFTFTSKHSARGNGTSSSESTPKSTKYVKE
RRPPPPPPLLYSTESIRIDSPMVSPSSQSRERSPNKLSFIGNSEERHHMEYISNHSRI
LKSPFANGFSPNSPKSPRDSSKQQAHFSDESDLRCHEREKALPPIPFTTTLLLSPFDD
EDSEFFTKPPPPLSTSRNVSGNSRVSEALESVYSDSDYTFNNSNARQSSFNSLLGAKP
LELAPSITAPTQPFSIQSIDEHKLYQCDNVYKLSAIYEWILKVYFEWFNECVFTKIDL
FQIVQLLLEFQMPTNFDQDTIDSNVDNIMASFISQKAVRFDIINDEEVAVVVGGLDIT
GVFTELLPCYSFIDNTYGSTNSLICYSNVCTHGQSSGFRKEIKLSEIINKSVGLWTEY
WHLTPDDLAEINPREVQRQSFIFDLIILEERSLNMATAAVEIYGKRFDKSLLPDEPEF
KALAFDIFEPLIQLHTEFLLTPIFWKLKTRGKFIDGVGKIYSKWCGEAKNIYLNYAKA
MATVHEIIMWEKKNKTKFVTWLKEIDNSVEITRSKMYHDVIFFGGFFKSLQNMPVTLR
SILKNTDPSMEDYEYLKIVIKEVEKLNFEVNQVHGLAIDHRKLVRFSKQLVLSTNSSN
ATSYVNVGGSTNANDDDAIQDKLALGLTYPERKLVLSGTVYKKRDLWLDPTPVYIALL
DNCLLITEEISKGETQKYKLIERPIPIDYLSLEKRKIPGTSKQPLRNYSQKEHKSPMH
NFSTPINSMRPLLKSSGNHMSTAYGDRKTSNTEISNANPNTDEFSFKIRNTATGESFK
FFTESAEVLNQWIDAIMESFKRNAENHDLNAFEFTVLSSEFAYFDKDAPVNLPVAPEG
SEIDVALKAYAQKANKDSCSWSKTTRILCCEDVKFEGRIYLFVATTDGIYVKYRDDYG
SGFVKILELNDVKRMEANVKLGLLFVLDNRKLCYFNISTVVSRYLAQGNTLDENCIVG
TVIRDKVRFFKIADDFGNSKHLFFERKGKIVILTPEFDQLTNQVKYFKFYKEYKLPSS
SNNILNNEIEDIAIFRKSFAVCTKKTVILYQDSFEDNGIVLPSFLNDKDMMAHLRHPH
LNSLPFKSATDSKKRPSIESLTEEAKKDIATCKAIPVNFFQISQSSFFALVYDEAVVK
INCYGEMSDWRKDILLLDFCCTGASFHGNHLILVGDNLIQIYDLKNVEQNLGELVPVQ
IIKGKKIKLASSERREKTILVLSHPNILNRQLLVACNPVAMADHQ"
gene 927133..928184
/locus_tag="R008_L13131"
mRNA join(927133..927212,927284..928184)
/locus_tag="R008_L13131"
/product="hypothetical protein"
CDS join(927133..927212,927284..928184)
/locus_tag="R008_L13131"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84624.1"
/translation="MNIDCLCRWVVLPLLRYPLLVALVLRWSLSDSISICLTIYTLLI
NAFLIANSYTKRSGQVAWKSLREFKNGIVLITGGSKGLGRAIVSQLLQDYSNLTILNV
DICPSSVRNTRVKDLICDLSDDEEVAALLNLLKRKYKNEIRLIVNNAGVRANFTGFSG
MERDNLDKIFKINTFAPLQFIQELAPSRHSTRQCYIVNIASILGILTPAKVAAYAASK
AALIAFHQSYSFELQNEGVRNIRTLLVTPGQLNTEMFAGFKPPRQFFAPVIDITTLAA
KIVRYCELGQRGQLNEPFYCSFAHLLMCVPYSLQRIVRSFSRIDCCLPDE"
gene 928499..930511
/gene="Mag2"
/locus_tag="R008_L13136"
mRNA 928499..930511
/gene="Mag2"
/locus_tag="R008_L13136"
/product="Mag2p"
CDS 928499..930511
/gene="Mag2"
/locus_tag="R008_L13136"
/note="Similar to YLR427W 'Cytoplasmic protein of unknown
function; induced in response to mycotoxin patulin;
ubiquitinated protein similar to the human ring finger
motif protein RNF10; predicted to be involved in repair of
alkylated DNA due to interaction with MAG1'"
/codon_start=1
/product="Mag2p"
/protein_id="EWG84625.1"
/translation="MVEPDMQKKASGSSGGSEMDTLSATSNSSKQGVSNNKRNPVSKK
KPGNKVSDGRDNAHNYHGEGRRKSSKQQRSRTPYKETSTRINDQDIDLSIQEEILGGN
FKLRGRKTQVSINHLLNFQLPEVEREKSRSSSSKKSNRRRDEHVHLHGDTFVNVNYRL
LVDDRFDYPEQNCNPNVPVDQEKILRVIVPKGQNCSICLSEEPVAPRMVTCGHIFCLS
CLLNFFSIEETVKNKETGYSKKKKYKECPLCGSIIGPKRVKPVLYEDDFDVTRLNQKP
EPGATVHLQLMCKPHGSLLPLPVALHLDPLKCGNFPPANLGSIKHYAHIMKCGVSYSL
ELYQKDIVAIQEQYEIDKAIYNDSGKFVKQSIENINDQISTLLAATTDLSPLSNDINN
GLNNFHFDDDLLTKYDDSSAYFFYQTLVASSTKYFLSPLDVKILLTIFHYYSKFPESI
ETTVENIHYDTVVTEQLIRRYKYIGHLPIGTEIALLDLDWRKIPFLPKEIYEQFAHEL
KQRRRKFTMKKQKEDKEKKLYEKRLEQEHAEFYRKENGNSLKFEDSVQMATHYESIVS
SSIPLNSLGISMLGPPTNSCSTPQKQAPSHTKRTIWGTSIAVTEDEKASKENKEFQDM
LLQRIRQEDSSDVTDSTDSPPTSNGKRGRKKKGKVMLFSSNHQALG"
gene 930848..932803
/gene="Crn1"
/locus_tag="R008_L13146"
mRNA 930848..932803
/gene="Crn1"
/locus_tag="R008_L13146"
/product="Crn1p"
CDS 930848..932803
/gene="Crn1"
/locus_tag="R008_L13146"
/note="Similar to YLR429W 'Coronin, cortical actin
cytoskeletal component that associates with the
Arp2p/Arp3p complex to regulate its activity; plays a role
in regulation of actin patch assembly'"
/codon_start=1
/product="Crn1p"
/protein_id="EWG84626.1"
/translation="MSGKFVRASKYRHVFGQAAKKELQYEKLKVTNNAWDSNLLKTNG
KFIAVNWNASGGGAFAVIPIEEVGKAPDQVPLFRGHTAQVLDTDFDPFNDHRIASGSD
DSKIGIWDIPENYKFHDHVDEDGEPIDIKPVKFLTGHARKVGHVLYHPVAENVLASSS
GDYTVKLWNVETGKDMITLKHPDMVTSMSFSYDGNYLATVARDKKLRVWNIREEKIVS
EGPAHTGAKNQRVVWLGNSDRLATTGFSKLSDRQIGIWDAFNIEKGDLGGFYTVDQSS
GILMPFYDEGNKILYLVGKGDGNIRYYEFQNDELFELSEFQSTEAQRGFAVAPKRMVN
VKENEVLKGFKTVVDQRIEPVSFFVPRRSEEFQEDIYPDAPSNKPALTAEEWFSGKSV
EGPILVSMRSIYDGSAPSFHEAKRPQQPTTQETALEEKKEQPKVEKPISESEKEVKQE
APKSPSPLKSASSSSTINHVLKEDNSINKLLKKSSDIDQVNNAEDPSRDTSGWEEADD
EPAPIKIETPVTPTETKKDRTPKVEPSKELKPEPVSIATDRKQEQSLPQEEKSSEKTK
SPEQEKSATPPSSITAAKTAITASSKEEPSAARTSPKSLGLKQSVEKLSTLVLQLEDV
VDKLTKANLDKDERLLKLEQKIGELSK"
gene 933505..940200
/gene="Sen1"
/locus_tag="R008_L13151"
mRNA 933505..940200
/gene="Sen1"
/locus_tag="R008_L13151"
/product="Sen1p"
CDS 933505..940200
/gene="Sen1"
/locus_tag="R008_L13151"
/note="Similar to YLR430W 'Presumed helicase required for
RNA polymerase II transcription termination and processing
of RNAs; homolog of Senataxin which causes
Ataxia-Oculomotor Apraxia 2 and a dominant form of
amyotrophic lateral sclerosis'"
/codon_start=1
/product="Sen1p"
/protein_id="EWG84627.1"
/translation="MNSNNPDNNNSNNINNNNKDKDIAPNSDVQLATVYTKAKSYIPQ
IEQVYQGTNPNIQEAKLLGELLQVLAEVPKGTHLFCDPILEPISIFSLTIFSFNEEAT
ATWLKNHFNPILSVCDKCILNFARGKCKMLQHFAIQRHVPHEHVAKFNDIVCQWRVEA
VFPILRNISVNDNTGINITNEIETAMYECLCNPHMLRLNKQLKATFEAIFKFFYDTKH
RLLDVTNPLSIKTFISGVIFCWCEGSKEENEWSRAFLKDLYSRNFHINLSNLTPDIIE
EVYIHILFLQNPANWTEIVVSQFWSRLLPVFNLFDKDVFIEYFQVPKNVESLKKTFKF
PLEPIFKMWYNHLSKSYHDKPLDFLLRGLTMFLNKFGSEFWSKIEPFTFHSILDIIFN
RDSFPIKLIKIQDNPIVEHQTEVYFQLTGSVTDLLSWTLPFYHALSPSKRIQMVRKVS
MAFLRIIANYPSLKSIPKACLMNSATALLRAVLTIKENERAMLYKNDEFETVLLTKTD
SRALLNNPLIQDIIIRSASNPNDFYPGLGAASASVATSTMMVLAECIDFDILLLCHRT
FKLYSGKPISEIPISTNVLENVTNKIDLRSFHDGPLLAKQLLVSLKNINGLLIVPSNT
AVAEAHNALNQKFLLLSTRLMEKFADILPGQLSKILADEDASQGFWSCIFSSDKHLYQ
AATNILYNTFDVEGRLEGILAILNSNLTVNLKNINVMLQRLINCEFYEPCPRAVRVLM
DVVSAFVDPISGVFANFQTLKSQNTEKEFLKFWESCWLFLDTIYKFTLKWASKYDYSE
LENFTKDTLDLSRSLVDSFREFSDILHDQTKNLLLNVLETFKNMLYWLRLSDEVLLES
CVRLIISTSDLAHEKHVKVDDSLVEMMAKYASKAKRFSNKLTEQQASEILQKAKIFNK
ALTEEVATEAENYRKEKELSRLGKVIDLTDSVPASPSLSPSLSSTIASSSAESRADYL
QRKALSSSITGRPRVAQPKITSFGTFQSSANAKLHRTKPVKPLSKMELARMQLLNNRV
VHPPSAPAFHTKSRGLSNKNDDSSSEESDNDIESARELFAIAKAKGKGIQTVDINGKV
VKRQTAAELAKQELEHMRKRLNVDMNPLYEIILQWDYTRNSEYPDDEPIGNYSDVKDF
FNSPADYQKVMKPLLLLESWQGLCSSRDREDYKPFSIIVGNRTAVSDFYDVYASVAKQ
VIQDCGISESDLIVMAYLPDFRPDKRLSSDDFKKAQHTCLAKVRTLKNTKGGNVDVTL
RIHRNHSFSKFLTLRSEIYCVKVMQMTTIEREYSTLEGLEYYDLVGQILQAKPSPPVN
VDAAEIETVKKSYKLNTSQAEAIVNSVSKEGFSLIQGPPGTGKTKTILGIIGYFLSTK
NASSSNVIKVPLEKNSSNTEQLLKKQKILICAPSNAAVDEICLRLKSGVYDKQGHQFK
PQLVRVGRSDVVNVAIKDLTLEELVDKRIGERNYEIRTDPELERKFNNAVTKRRELRG
KLDSESGNPESPMSTEDISKLQLKIRELSKIINELGRDRDEMREKNSVNYRNRDLDRR
NAQAHILAVSDIICSTLSGSAHDVLATMGIKFDTVIIDEACQCTELSSIIPLRYGGKR
CIMVGDPNQLPPTVLSGAASNFKYNQSLFVRMEKNSSPYLLDVQYRMHPSISKFPSSE
FYQGRLKDGPGMDILNKRPWHQLEPLAPYKFFDIISGRQEQNAKTMSYTNMEEIRVAI
ELVDYLFRKFDNKIDFTGKIGIISPYREQMQKMRKEFARYFGGMINKSIDFNTIDGFQ
GQEKEIILISCVRADDTKSSVGFLKDFRRMNVALTRAKTSIWVLGHQRSLAKSKLWRD
LIEDAKDRSCLAYACSGFLDPRNNRAQSILRKFNVPVPSEQEDDYKLPMEYITQGPDE
VKSNKDTKKRRVVDEGEEADKAVKKKKKEKKKEKKKSKADDKKKNNKKAESPSTSSGT
KKKSSIFGGMSVPSAVVPKTFPDVDSNKKAAAVVGKKKNNKHVCFSDDVSFIPRNDEP
EIKVTRSLSSVLKEKQLGLKETRTISPPEISNNEDDDDEDDYTPSISDSSLMKSEANG
RNNRVASHNQNFSASIYDDPQVSQAKQTQVPAAITKHRSSNSVLSGGSSRILTASDYG
EPNQNGQNGANRTLSQHVGNANQYSTAPVGTGELHETLPAHPQDSYPAEAEDPYDLNP
HPQPQSSAFKGPGSGPTGTRNSSRRNASSSPFIPKKRKPRS"
gene complement(940413..941774)
/gene="Atg23"
/locus_tag="R008_L13156"
mRNA complement(940413..941774)
/gene="Atg23"
/locus_tag="R008_L13156"
/product="Atg23p"
CDS complement(940413..941774)
/gene="Atg23"
/locus_tag="R008_L13156"
/note="Similar to YLR431C 'Peripheral membrane protein
required for the cytoplasm-to-vacuole targeting (Cvt)
pathway and efficient macroautophagy; cycles between the
phagophore assembly site (PAS) and non-PAS locations;
forms a complex with Atg9p and Atg27p'"
/codon_start=1
/product="Atg23p"
/protein_id="EWG84628.1"
/translation="MELNQVLEKKEQILQYLGTLVGLHEKALSDVNSASQVTSIRKDI
TICLNDLCRINDLLVSHDGLLKREIGSLLRDKQELLELNEREQLLWKERKSWHIKQET
DAAPADYVIDKDAIITISSHHRTSLNKYIESVGAENTILSNTDDSDAMIEEVQNAESS
ADQMIRNYKLLQLSHKQAKSEIIRLETLLRDFKKDNKFIEEELKRQSGRIRSEMANID
FHLSKIEESKHQLMKRIGFESPLTQEKSLSEKIFNLRLSSADEDYNERQTINMKNFVH
MKDLIELKIEDLQEQLMRNKNESSTVLTQRELWLDCQKKVGDLESKLITKLRSSSNSK
IPPNEMSEMINSTIQYLNNLLDSSDEKLTTTLISNERDVLSKACEELHSESTTAQDGS
SALPSKPIDIHKSHKGSNASSNLKQPSTPSFLVASKSPPKIGISESVVNANKNDAISK
KVE"
gene 942630..944201
/gene="Imd3"
/locus_tag="R008_L13161"
mRNA 942630..944201
/gene="Imd3"
/locus_tag="R008_L13161"
/product="Imd3p"
CDS 942630..944201
/gene="Imd3"
/locus_tag="R008_L13161"
/note="Similar to YLR432W Inosine monophosphate
dehydrogenase, catalyzes the first step of GMP
biosynthesis, member of a four-gene family in S.
cerevisiae, constitutively expressed"
/codon_start=1
/product="Imd3p"
/protein_id="EWG84629.1"
/translation="MAAVRDYKTALEFAKSLPRLDGLSVQELMDSKTRGGLTYNDFLV
LPGLVDFPSSEVSLQTKLTRNITLNTPFVSSPMDTVTESEMAIFMALLGGIGFIHHNC
TPEDQADMVRRVKNYENGFINNPIVISPTTTVGEAKSMKERFGFSGFPVTEDGKRNGK
LMGIVTSRDIQFVEDNSLLVQDVMTKNPVTGAQGITLSEGNEILKKIKKGKLLIVDDN
GNLVSMLSRTDLMKNQNYPLASKSATTKQLLCGAAIGTIDADKERLRLLVEAGLDVVI
LDSSQGNSIFQLNMIKWIKETFPDLEIIAGNVATREQAANLIAAGADGLRIGMGSGSI
CITQEVMACGRPQGTAVYNVCEFANQFGIPCMADGGVQNIGHITKALALGSSTVMMGG
MLAGTTESPGEYFYQDGKRLKAYRGMGSIDAMQKTGTKGNASTSRYFSESDSVLVAQG
VSGAVVDKGSIKKFIPYLYNGLQHSCQDIGYKSLTLLKENVQSGKVRFEFRTASAQLE
GGVHNLHSYEKRLHN"
gene complement(944420..946081)
/gene="Cna1"
/locus_tag="R008_L13166"
mRNA complement(944420..946081)
/gene="Cna1"
/locus_tag="R008_L13166"
/product="Cna1p"
CDS complement(944420..946081)
/gene="Cna1"
/locus_tag="R008_L13166"
/note="Similar to YLR433C 'Calcineurin A; one isoform (the
other is CMP2) of the catalytic subunit of calcineurin, a
Ca++/calmodulin-regulated protein phosphatase which
regulates Crz1p (a stress-response transcription factor),
the other calcineurin subunit is CNB1'"
/codon_start=1
/product="Cna1p"
/protein_id="EWG84630.1"
/translation="MSKDLNSSRIKIIKPNDSYIKVDRKKDLTKYELENGKVISTKDR
PIASVPAITGKIPSDEEVFDSKTGLPNHSFLREHFFHEGRLSKEQAIKILNMSTVALS
KEPNLLKLKAPITICGDIHGQYYDLLKLFEVGGDPAEIDYLFLGDYVDRGAFSFECLI
YLYSLKLNNLGRFWMLRGNHECKHLTSYFTFKNEMLHKYDMEVYDACCRSFNVLPLAA
LMNGQYFCVHGGISPELKSVEDVNKINRFREIPSRGLMCDLLWADPVENYDDARDGSE
FDQSEDEFVPNSLRGCSFAFTFKASCKFLKANGLLSIIRAHEAQDAGYRMYKSNKVTG
FPSLITMFSAPNYLDTYHNKAAVLKYEENVMNIRQFHMSPHPYWLPDFMDVFTWSLPF
VGEKVTSMLVSILNICSEQELDPESEPKAAEETVKARANATKETGTPSDEKASSAILE
DETRRKALRNKILAIAKVSRMFSVLREESEKVEYLKTMNAGVLPRGALARGTEGLNET
LSTFEKARKEDLINEKLPPSLSEVEQEKIKYYEKILKGAEKKPQL"
gene 946451..947068
/gene="Tsr2"
/locus_tag="R008_L13176"
mRNA 946451..947068
/gene="Tsr2"
/locus_tag="R008_L13176"
/product="Tsr2p"
CDS 946451..947068
/gene="Tsr2"
/locus_tag="R008_L13176"
/note="Similar to YLR435W Protein with a potential role in
pre-rRNA processing"
/codon_start=1
/product="Tsr2p"
/protein_id="EWG84631.1"
/translation="MSTQYIDETAFVQAEQGKTNLMFSDEKQQARFELGVSMVIYKWD
ALDVAVENSWGGPDSAEKRDWITGIVVDLFKNEKVVDAALIEETLLYAMIDEFETNVE
DDSALPIAVEVINIYNDCFNLNYNKVEKLYLEWQEKQRTKKSKRVVHIEGDDDEDDED
EEDYDDEDEDEEMDEVVPDLVSSKPEPIVDEDGFELVQPKGRRKH"
gene complement(947514..951338)
/gene="Ecm30"
/locus_tag="R008_L13181"
mRNA complement(947514..951338)
/gene="Ecm30"
/locus_tag="R008_L13181"
/product="Ecm30p"
CDS complement(947514..951338)
/gene="Ecm30"
/locus_tag="R008_L13181"
/note="Similar to YLR436C 'Putative protein of unknown
function; may play a role in cell wall biosynthesis,
mutants have abormal relative levels of mannose and
glucose and have Gap1p sorting and transport defects;
(GFP)-fusion protein localizes to the cytoplasm'"
/codon_start=1
/product="Ecm30p"
/protein_id="EWG84632.1"
/translation="MGNTDSKSSSILLNHCIALVRPEDASASSPSRTSSPSPSLSVDA
DPLSLNLSIFKLDSGPDVEALFSDKPNVPLDTVFNDFYLDFISVDVQDFSINSSFKKI
LQIISSLNPPNFNNLIVFLSLYIILSANSLPASRTGLHSSRLINAIKTLSILIPIYFD
RVKSSTQDHYDVFWATQHEIEGLPLQNIPLGERLLLAILKLAFQDNFTTAVTAHPSEL
WEIGILTNSNKYRSLLNMHHQWHLFANRLLLLRLLAALFSSDLYTSGGKQDINMFLVY
WCTQMPKDKSIQFTSSLLNCTMRFILNNNKDFQSLKANFFSSDATASNWQTLYFQFVQ
SCLHVLNLSMSYKAQDNVITIFLTQLQREYDLKLILSSFIKIFKYPIDLAIEQESNIF
NFTNNKHIDASRRRAVSTSSHDNSSSSHASLPSSSSAAYHTKPQTKPQLPEIHPLLIP
MTILMTNLIDCNKCFQNYFADKFASRFIIFSIYYLKYYDYSSLSSSSSTTRSNSSTTS
NGTSNDTSNERSIVELNENSVSQILLPLLNHLLLILTSKKLVLFKMLQTFNLNYYTNN
LPNFYKLSNINGDINNLTFRDFTIIQLSNLILDNIKFNLQPNPIFYELIYNLLPINDE
ILTSSHKNDDSHDDLILLSAKKKSASPSAATSSHTSSSKLSYNAAMSLLYVLSKSSNK
VYLTTYATPVFKTKDIPYMISPGFKMDLLALLLRSITIFFTLYFDDAENLLFAMVRHQ
SITHQINDSINSISKALDMNPNLNSHIMTLKQMGFNRKVQWKDFYQFEEITDLPQVNL
YSSANQQHQNQQQGQNDNRGQNQNEDPGQENESPTPYLLFNPASLENETSGTVKHFSS
TNHDKNYQVIAFIDFKSDSNLNLQHQLEYWPHRPQWPTPLTFTHKCKNPKYENFNEVW
SGTVYLQILLRVIKQILSKVPEIPRIKSVQYFETLSKLSALRSDILTTIHPRLPLDVR
RLTTFQPLSMHTNDKLLMWFHIATWANIFTQTSFKYEETFSHELRQFESLLDISIDEC
EGNTISKPTTDRLGYIRRSRGQSSVSLERTISAGSGVSTPTMALNRTKSNGSGNLMNY
FFQNTAQNHFQHLRSSSSSSSITLEKTTSNSSSIRTRPNSHHVAPETNNNNSTNGNSN
NSSNGGFSFFKWKWGGNNSNGGSDDTKASQRDPNVSTSIITDNLNSYMFEEEISPGVV
NNIIENNIWVGTDIRLFKIANFRKESFSFLEMTSSFFKKFKFINSDNDNYNNNEFDDN
TQLRYTSRGLYR"
gene complement(951711..952112)
/locus_tag="R008_L13191"
mRNA complement(951711..952112)
/locus_tag="R008_L13191"
/product="hypothetical protein"
CDS complement(951711..952112)
/locus_tag="R008_L13191"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84633.1"
/translation="MDAQLEWASSLVPKRQLQQQQQQQEQQQQQQQDFHKDQLMTVGM
RIRQRVDQGYASRTPSTSDASLQPGVIRDYSSVIVPQFTRSPLPTANSLPPMLINQRT
MSTEASSLEKWDVAEPAAEHEAMVNGSKRRL"
gene complement(952552..952779)
/locus_tag="R008_L13196"
mRNA complement(952552..952779)
/locus_tag="R008_L13196"
/product="hypothetical protein"
CDS complement(952552..952779)
/locus_tag="R008_L13196"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84634.1"
/translation="MAMALVDGRISAQEVEVQLTLRVPHMCALSLVKNDGQGMVVVGG
VFVFPFNGLLGGEGSFGHWCCGSVLLWCLVV"
gene 952594..953878
/gene="Car2"
/locus_tag="R008_L13201"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(953929..954288)
/gene="Lsm3"
/locus_tag="R008_L13206"
mRNA complement(953929..954288)
/gene="Lsm3"
/locus_tag="R008_L13206"
/product="Lsm3p"
CDS complement(953929..954288)
/gene="Lsm3"
/locus_tag="R008_L13206"
/note="Similar to YLR438C-A no_gene_description"
/codon_start=1
/product="Lsm3p"
/protein_id="EWG84635.1"
/translation="METPLDLLKLNLDERVYIKLRGARTLVGTLQAFDSHCNIVLSDA
VETIYQLNNEELSESERRCEMVFIRGDTVTLISTPSEDDDGAVEICGDISKEEEKTKG
NSTYFFFTFSILKMCKI"
gene 954615..955574
/gene="Mrpl4"
/locus_tag="R008_L13211"
mRNA 954615..955574
/gene="Mrpl4"
/locus_tag="R008_L13211"
/product="Mrpl4p"
CDS 954615..955574
/gene="Mrpl4"
/locus_tag="R008_L13211"
/note="Similar to YLR439W 'Mitochondrial ribosomal protein
of the large subunit, homolog of prokaryotic L29 ribosomal
protein; located at the ribosomal tunnel exit'"
/codon_start=1
/product="Mrpl4p"
/protein_id="EWG84636.1"
/translation="MWKRSFHSQGGPLRARTKFTKPKPKQPVLPKDKIRPPTQLTHHS
NNLRITEPIPPTTSNLRCPDDHPLWQFFSNKKFIRSADDLPPSSHIRPWSIPELRHKS
FNDLHSLWYNCLREQNVLARENHLLKNIVGSTHDEFSELSNSIRTTMWQIRHVLNERE
LAYSASRKFLQDESERKKFLDTLANDYFLNKDIPDDEVASMLTRFQLAIFGISETIQD
NTVDINFIDGIKFLANLKLQRFKDSNDLISEISQEPITDVGESFILFTSDFEPHAVQE
ACVAIKDLRKSPDNKVPKLDELPTVRKYLKQLIHASSVEQATA"
gene complement(955694..957823)
/gene="Sec39"
/locus_tag="R008_L13216"
mRNA complement(955694..957823)
/gene="Sec39"
/locus_tag="R008_L13216"
/product="Sec39p"
CDS complement(955694..957823)
/gene="Sec39"
/locus_tag="R008_L13216"
/note="Similar to YLR440C 'Component of the Dsl1p
tethering complex that interacts with ER SNAREs Sec20p and
Use1p; proposed to be involved in protein secretion;
localizes to the ER and nuclear envelope'"
/codon_start=1
/product="Sec39p"
/protein_id="EWG84637.1"
/translation="MLEEQLYLLACIFASRADTRNIKKLSTRLGSQSNYLEILCVLWP
ELDDPKNLLFLRELEEEVQSPEGEETTDEDVIVELLENDSSLIPLIESDTTTRSNRYH
ELQEFISKKLNNKTLENFVEWLRERILICNEMIPETPLLYSVLWETAKSKVLSTKFIG
WVEGVLKPLDHLNKRLHLIFKINEWEKMPDSELFKIIFDGVEDMQEYIGIADVIEYEL
APTLSYGKKWETFITEFFNKRQFSLKSDTNYQLFIKLYYSLEKGVKDNSEASRKLQSN
VVDILFHNSENLFNLSSLTHKLDELWSILSGFPDEITIEEQKTITALEMKQFMEFFIK
CSTKFSFKEIFAITQEEESAQLAHFSSLCHEEFNKANEISSFLQAMYETVLDISKDDK
IFTRISMDEKLYSILEILLQMNEFAYIEAIIERFDYSNNTQIYELLVKFFWHFFNNAS
NGLRKEPEMKKASQTLQIIQKHMSQRAGTNLTKLEVLLEISDKLSHYSINLNKSYNGA
RDTAFKPSNILEYRDCPLDIISNLLELNPRLYKDLPTTKSLLFGIYDSLSINREGQTG
KVEVDLMVLHIDYALVNLDFGTAYELGKQVFEICQEAGQHMMKALGDEHWLTFYQMGK
FVDPNWVDNEIPTEIIVLQMSILGRLLEVCPLEEVEIVTSQWSTLELELSARDLVKDK
YALDGQNDNKSKVGGIAREIFHNVTNF"
gene complement(958268..959035)
/gene="Rps1a"
/locus_tag="R008_L13221"
mRNA complement(958268..959035)
/gene="Rps1a"
/locus_tag="R008_L13221"
/product="Rps1ap"
CDS complement(958268..959035)
/gene="Rps1a"
/locus_tag="R008_L13221"
/note="Similar to YLR441C 'Ribosomal protein 10 (rp10) of
the small (40S) subunit; nearly identical to Rps1Bp and
has similarity to rat S3a ribosomal protein'"
/codon_start=1
/product="Rps1ap"
/protein_id="EWG84638.1"
/translation="MAVGKNKRLSKGKKGQKKRVVDPFTRKEWFDIKAPSTFENRNVG
KTLVNKSTGLKSASDALKGRVVEVCLADLQGSEDHSFRKIKLRVDEVQGKNLLTNFHG
MDFTTDKLRSMVRKWQTLIEANVTVKTSDDYVLRIFAIAFTRKQANQVKRHSYAQSSH
IRAIRKVISEILTKEVQGSTLAQLTSKLIPEVINKEIENATKDIFPLQNIHVRKVKLL
KQPKFDVGALMALHGEGSGEEKGKKVTGFKDEVLETV"
gene complement(959434..962369)
/gene="Sir3"
/locus_tag="R008_L13226"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 962743..964089
/gene="Ecm7"
/locus_tag="R008_L13231"
mRNA 962743..964089
/gene="Ecm7"
/locus_tag="R008_L13231"
/product="Ecm7p"
CDS 962743..964089
/gene="Ecm7"
/locus_tag="R008_L13231"
/note="Similar to YLR443W 'Non-essential putative integral
membrane protein with a role in calcium uptake; mutant has
cell wall defects and Ca+ uptake deficiencies;
transcription is induced under conditions of zinc
deficiency'"
/codon_start=1
/product="Ecm7p"
/protein_id="EWG84639.1"
/translation="MVMSRIRDTIARPFQNLTALEKVVQWLRLGTTLLIISFGLALTV
GPLSSPRTLYMSRLDTYSADITTGLFTVLRESMEQSTSTEENNGVGLTTSELYILTAY
TESQIKNVPQYITVSLYGRCDSTYTMVEVFDSEGNMHSVKNSTTKSTCSSIGTDYLFD
YREVLESLGLDIILDYAYNKIGSQQAESSAYTTYMRSLKHKKANVLHLLYAVISFQVC
MLFFMIWYYYIKGRFMNALKERALVHINSLLSLVVFIGGLISSISLAWVNYTIQSRIN
TELEAFGFSYHLGVTWFALLWCFAGLISVSYLAWSGLEWCISDNGTSYGGGIDDKFLG
YQAGVFTDADLDDETSYSQRYPQRQSTSGEAELMRNSDTMATIRKTSDVDLNSENDAN
TSLDHGNHTANISNGGKHEPFATREEFELQDIRFRSSNDSEESMQRVIKPSSALQF"
gene 964307..964955
/locus_tag="R008_L13246"
mRNA join(964307..964690,964773..964955)
/locus_tag="R008_L13246"
/product="hypothetical protein"
CDS join(964307..964690,964773..964955)
/locus_tag="R008_L13246"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84640.1"
/translation="MSDTTEVPRQSSENDQDNNLEKTNSSKSPDVTNNIPSLFKLAAE
WQINNPQETFQNHILENDVLKKINEITHLIRESYKDLSSQDGMMSKQQQEKMDWDLFC
TVPVKIIEQYTKDMDEIYEKMERLAKQQRLWCESAFQIDVERCGDSILNAETWMKKKE
RHLEYKNIEMERSANEIKETIQRLTDDR"
gene 965353..966654
/locus_tag="R008_L13251"
mRNA 965353..966654
/locus_tag="R008_L13251"
/product="hypothetical protein"
CDS 965353..966654
/locus_tag="R008_L13251"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84641.1"
/translation="MTIKSTLARELESLILPADSIVNVVDQFQEELLSRLQTNTISML
PQCLVPDKRSRWNPEDKILTIDFGGTRLKFAIISLPQIVIEYNDAFELTYNIVDSNFF
NQIIYTICTRLAANGYIKKKNESSEASKFFVSVTFSFPLNPEGEVVAMGKGFVMTDTL
QGSTVKQLIQSSFDRIISENIEEFFCTMNVCHVINDAIAVSLTSKFICENDSISLIIG
TGTNACFEVPYGYLPPFKRDALRETLPSSYNKETLNFKHVLINSEIGFIGKNVIALQP
FDIHGAISYEMPLECVTSGKLLPLSLKNILLQYNIIPKNFPVEFNGELVCQLAEGSTN
AWFENEHYALICQIARLLIKRAAFYVAAIVQAIDIITGCKNYNFIHIGYVGSFLHNSN
FYREQIKYYSSIHIKLQFLNHSNLLGAAIATYLNKSDNQVQ"
gene complement(966994..968031)
/gene="Vma6"
/locus_tag="R008_L13261"
mRNA complement(966994..968031)
/gene="Vma6"
/locus_tag="R008_L13261"
/product="Vma6p"
CDS complement(966994..968031)
/gene="Vma6"
/locus_tag="R008_L13261"
/note="Similar to YLR447C 'Subunit d of the five-subunit
V0 integral membrane domain of vacuolar H+-ATPase
(V-ATPase), an electrogenic proton pump found in the
endomembrane system; stabilizes VO subunits; required for
V1 domain assembly on the vacuolar membrane'"
/codon_start=1
/product="Vma6p"
/protein_id="EWG84642.1"
/translation="MEGVYFNIDNGFIEGVVRGYRNGLLSNNQYINLTQCDTLEDLKL
QLSSTDYGNFLSSVSSESLTTSLIQEYASSKLYHEFNYIRDQSSGSTRKFMDYITYGY
MIDNVALMITGTIHDRDKGEILQRCHPLGWFDTLPTLSVATDLESLYETVLVDTPLAP
YFKNCFDTAEELDDMNIEIIRNKLYKAYLEDFYNFVTEEIPEPAKECMQTLLGFEADR
RSINIALNSLQSSDIDPDLKSDLLPNIGKLYPLATFHLAQAQDFEGVRAALANVYEYR
GFLETGNLEDHFYQLEMELCRDAFTQQFAISTVWAWMKSKEQEVRNITWIAECIAQNQ
RERINNYISVY"
gene 968992..969907
/gene="Rpl6b"
/locus_tag="R008_L13266"
mRNA join(968992..969006,969392..969907)
/gene="Rpl6b"
/locus_tag="R008_L13266"
/product="Rpl6bp"
CDS join(968992..969006,969392..969907)
/gene="Rpl6b"
/locus_tag="R008_L13266"
/note="Similar to YLR448W 'Protein component of the large
(60S) ribosomal subunit, has similarity to Rpl6Ap and to
rat L6 ribosomal protein; binds to 5.8S rRNA'"
/codon_start=1
/product="Rpl6bp"
/protein_id="EWG84643.1"
/translation="MTAQQAPKWYPSEDVAAPKKTRKAVRPQKLRASLVPGTVLILLA
GRFRGKRVVYLKHLEDNTLLVTGPFKVNGVPLRRVNARYVIATSTKVSVEGVNVEKFN
VEYFAKEKLTKKEKKEANLFPEQQTKEIKTERVEDQKVVDKALLAEIKKTPLLKQYLS
ASFSLKNGDKPHLLKF"
gene 970975..972153
/gene="Fpr4"
/locus_tag="R008_L13276"
mRNA 970975..972153
/gene="Fpr4"
/locus_tag="R008_L13276"
/product="Fpr4p"
CDS 970975..972153
/gene="Fpr4"
/locus_tag="R008_L13276"
/note="Similar to YLR449W 'Peptidyl-prolyl cis-trans
isomerase (PPIase) (proline isomerase) localized to the
nucleus; catalyzes isomerization of proline residues in
histones H3 and H4, which affects lysine methylation of
those histones'"
/codon_start=1
/product="Fpr4p"
/protein_id="EWG84644.1"
/translation="MSDMLPLATYSLNVEPYSPTPALNFKIPVTIRITMAAIDPEPFD
DDKKPSTLRIIKRNPELTRGEYYNQDNNDGLEEDESESEQEADVPKRSVKSKKGKAVE
QSESEDSEDENEIDDEFEECVLLTLSPKGQYQQALDITIAPEEDVQFVVTGSYTISLT
GNYVKHPFDNSSDSDEDEKDYYSDEESSNGEEEEEEEEEDDEELSSGDDDLDDLVDAS
DIESRLDELVEKDEKKKNNKKDSKRKHEEDEEESAKPAEKKQTTKKDKKAEKVKDSEE
SKPKPKTKLLEGGIIIEDRVTGKGPHAKKGTRVGMRYVGKLKNGKVFDKNTKGKPFVF
KLGQGEVIKGWDIGVAGMAVGGERRIVIPAPYAYGKQALPGIPANSELTFDVKLVSMK
"
gene 972768..975905
/gene="Hmg2"
/locus_tag="R008_L13281"
mRNA 972768..975905
/gene="Hmg2"
/locus_tag="R008_L13281"
/product="Hmg2p"
CDS 972768..975905
/gene="Hmg2"
/locus_tag="R008_L13281"
/note="Similar to YLR450W 'One of two isozymes of HMG-CoA
reductase that convert HMG-CoA to mevalonate, a
rate-limiting step in sterol biosynthesis; overproduction
induces assembly of peripheral ER membrane arrays and
short nuclear-associated membrane stacks'"
/codon_start=1
/product="Hmg2p"
/protein_id="EWG84645.1"
/translation="MSLPLKTIVHLVKPFACTARFSARYPIHVIVVAVLLSAAAYLSV
TQSYLNEWKLDSNQYSAYLSIKPDELFEKCTHYYRSPVSDTWKLLSSKEAADIYTPFH
YYLSTISFQSKDNSTTLPSLDDVIYSVDHTRYLLSEEPKIPTELVSENGTKWRLRNNS
NFILDLHNIYRNMVKQFSNKTSEFDQFDLFIILAAYLTLFYTLCCLFNDMRKIGSKFW
LSFSALSNSACALYLSLYTTHSLLKKPASLLSLVIGLPFIVVIIGFKHKVRLAAFSLQ
KFHRISIDKKITVSNIIYEAMFQEGAYLIRDYLFYISSFIGCAIYARHLPGLVNFCIL
STFMLVFDLLLSATFYSAILSMKLEINIIHRSTVIRQTLEEDGVVPTTADIIYKDETA
SEPHFLRSNVAIILGKASVIGLLLLINLYVFTDKLNATILNTVYFDSTIYSLPNFINY
KDIGNLSNQVIISVLPKQYYTPLKKYHQIEDSILLIIDSVSNAIRDQFISKLLFFAFA
VSISINVYLLNAAKIHTGYMNFQPQSNKIDDLVVQQKSATIEFSETRSMPASSGLETP
VTAKDIIISEEIQNNECVYALSSQDEPIRPLSNLVELMEKEQLKNMNNTEVSNLVVNG
KLPLYSLEKKLEDTTRAVLVRRKALSTLAESPILVSEKLPFRNYDYDRVFGACCENVI
GYMPIPVGVIGPLIIDGTSYHIPMATTEGCLVASAMRGCKAINAGGGATTVLTKDGMT
RGPVVRFPTLIRSGACKIWLDSEEGQNSIKKAFNSTSRFARLQHIQTCLAGDLLFMRF
RTTTGDAMGMNMISKGVEYSLKQMVEEYGWEDMEVVSVSGNYCTDKKPAAINWIEGRG
KSVVAEATIPGNVVKSVLKSDVSALVELNISKNLVGSAMAGSVGGFNAHAANLVTALF
LALGQDPAQNVESSNCITLMKEVDGDLRISVSMPSIEVGTIGGGTVLEPQGAMLDLLG
VRGPHPTEPGANARQLARIIACAVLAGELSLCSALAAGHLVQSHMTHNRKTNKANELP
QPSNKGPPCKTSALL"
gene 976234..978894
/gene="Leu3"
/locus_tag="R008_L13291"
mRNA 976234..978894
/gene="Leu3"
/locus_tag="R008_L13291"
/product="Leu3p"
CDS 976234..978894
/gene="Leu3"
/locus_tag="R008_L13291"
/note="Similar to YLR451W 'Zinc-finger transcription
factor that regulates genes involved in branched chain
amino acid biosynthesis and ammonia assimilation;
positively regulated by alpha-isopropylmalate, an
intermediate in leucine biosynthesis'"
/codon_start=1
/product="Leu3p"
/protein_id="EWG84646.1"
/translation="MEGRSDSVATSQSGSEMSHSETRNRTGMNARKRKFACVECRQQK
SKCDAHERAPEPCTKCAKKNVPCILKRDFRRTYKRARNEAIEKRFKELTRTLTNLTSD
EILKKIEEEQEIVLDNSNFTKEKVKQLRKSAFETTEIEPRSYKTLRGEPISYSTNRKH
TDSSPLTLLSSSTNFDPVHSTNVMTDDQLKCLPKSLGDVYLSSSDIAELFQEFATKYH
QFLPVVDLSKGAERIYHLSPCLFWVILLIGLRRKFWATDLMTRLSVLVKSVLSEITIS
PIIRYTPSDKDEPVLNVASVYSVQAFLLYTFWPPLTSSLSADTSWNTIGTAMFQALRV
GLNCAGFSKEYASANSELVNEQIRTWICCNVVSQTVASSFGFPAYVSFDYLVISSIRV
PNSKSQVDIPNELRQMAQIARFENQIVNTMNSTPASVTGMVSQEEKQPLLHVLNQQLS
QLEISLEENNLDDIRKFLLLVAKVHLLTYYFTDVTSQSAGKSNGNIYEGSYSIMELDT
SFETKRGLVKVYNAAVNFLIHANSMWEHDPTIIKYFPGLFVLNIWQSACIISKLIHSS
LHSMLDVNSGKKAYNNAISLTFNASVLKYDMAYRSSGIMRSIWSLFANMYDAWKNDQK
EGGGRLNNDFNLGITIKSRMSVNVFFDCLYILKEKCGMAKLERETKVSTAYNVDEEEE
EDEDEEGEEEEEEEELSSKVLENMDSQQLRTRKFTNVRHPEKKARKIIETIPLDPNPI
NAGSTSSGSSLTTPNSKVANTISYRGILNKMSPREQLNHANLDSSVSTDIKDTEAVNE
PLPIGRNAEHPANQPPLSITQMQENTLPATQANSSLLETYPIVQSNPVTTTIKESPNS
IMAGWDNWESDMVWRDVDILMNEFAFNPKV"
gene complement(979411..981507)
/gene="Sst2"
/locus_tag="R008_L13296"
mRNA complement(979411..981507)
/gene="Sst2"
/locus_tag="R008_L13296"
/product="Sst2p"
CDS complement(979411..981507)
/gene="Sst2"
/locus_tag="R008_L13296"
/note="Similar to YLR452C 'GTPase-activating protein for
Gpa1p, regulates desensitization to alpha factor
pheromone; also required to prevent receptor-independent
signaling of the mating pathway; member of the RGS
(regulator of G-protein signaling) family'"
/codon_start=1
/product="Sst2p"
/protein_id="EWG84647.1"
/translation="MVDKNRTLHELSSKNFSRTPNGLIFTNDLKTVYSIFLICLDLKE
KKHSSDTKSFLLTAFTKHFHFTFTYQEAIKAMGQLELKVDMNTTCINVSYNIKPSLAR
HLLTLFMSSKLLHTPQDRTRGEPKEKVLFQPTPKGVAVLQKYVRDIGLKTMPDILLSS
FNSMKLFTFERSSVTDSIIHSDYLIHILFIKMMGAKPNVWSPTNADDPLPCLSSLLEY
TNNDDTFTFEKSKPEQGWQAQIGNIDINDLERVSPLAHRFFTNPDSESHTQYYVSNAG
IRLFENKTFGTSKKIVIKYTFTTKAIWQWIMDCTDIMHVKEAVSLAALFLKTGLIVPV
LLQPSRTDKKKFQISRSSFFTLSKRGWDLVSWTGCKSNNIRAPNGSTIDLDFTLRGHM
TVRDEKKTLDDSEGFSQDMLISSSNLNKLDYVLTDPGMRYLFRRHLEKELCVENLDVF
IEIKRFLKKMTILKKLIDSKHCDKKSNTSTSKNNIVKTIDSALMKQANECLEMAYHIY
SSYIMIGSPYQLNIHHNLRQNISDIMLHPHSPLSEHFPTNLYDPSPASAESAASSISS
TEADTLGEPPEVSLKPSKNLSNENCSFKKQGFKHQLKEYKPAPLTLAETHSPNASVEN
SHTIVRYGMDNTQNDTKSVDSFPATLKVLRKLYPLFEIVSNEMYRLMNNDSFQKFTQS
DVYKDASALIGIQEKC"
gene complement(981940..983127)
/gene="Rif2"
/locus_tag="R008_L13301"
mRNA complement(981940..983127)
/gene="Rif2"
/locus_tag="R008_L13301"
/product="Rif2p"
CDS complement(981940..983127)
/gene="Rif2"
/locus_tag="R008_L13301"
/note="Similar to YLR453C 'Protein that binds to the Rap1p
C-terminus and acts synergistically with Rif1p to help
control telomere length and establish telomeric silencing;
deletion results in telomere elongation'"
/codon_start=1
/product="Rif2p"
/protein_id="EWG84648.1"
/translation="MEHVDSDFAPIRRSKKVVDSDKIVKAISDDLEQKNFTVLRKLNL
VPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVPSLSKFLSKN
LRNMKNFIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEGIQSSKSK
ECIESMGQASYSGQLDFEAREKLSNHTSDLMMMVMRKINNDESIDHIVYFKFEQLDKL
STSTIIEPSKFTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTKYTV
FEMPILTCAQEQEYLKKMIKFTFDFGNKLLQSYNSLVTCQLNNKESNLAIFFEFLKVF
PHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKV
HDR"
gene 984140..992026
/gene="Fmp27"
/locus_tag="R008_L13311"
mRNA 984140..992026
/gene="Fmp27"
/locus_tag="R008_L13311"
/product="Fmp27p"
CDS 984140..992026
/gene="Fmp27"
/locus_tag="R008_L13311"
/note="Similar to YLR454W 'Putative protein of unknown
function; the authentic, non-tagged protein is detected in
highly purified mitochondria in high-throughput studies'"
/codon_start=1
/product="Fmp27p"
/protein_id="EWG84649.1"
/translation="MMFPINVLLYKWLIFAVTFLWSCKILLRKLLGINITWINLFKLE
ICGLSLEDGTVRLKSVRFAVFERKLFIKGLRIDSKKSSTNDLHKELPREEERTFIETP
EDNGGGFISKILSLSQYWLNGVTIILEDTQLVNNDITIEKFGFFLSIDNSKHIKSLRF
DSFLRKLLWNGQTIIADAIFIVNTNLLIGEIMNPLKDGLQVGLDLKLGDLNIPMNLLN
LFINKENVDLMSNEKLLQRLADTTKANEELKDEDIAKMKDDLVYAMEKFVDRIKPLKE
MNVTIDKLQIKDFPLTNHPELLGMNKYISYNVLVSNINFNTNRFRNEMPGYTLIFEER
DSPFKFSIIMARFNIYLNLNRKHQSHAKQLKIIEIPNVSIFGETNLFSQKFRLSNNLH
AKELENAIFNIKGNISSLTIDMDPVNISFIKCFLSNIKVFTSSCPKNKILKENSHVKF
LTRRRVLFDYFKCFLPLINMKFTLDDPKFVINDKDDLIIGKFSVFMISHHSKRYTLGN
NLMEEKEETQHIFYESHWNVELLDMKLQHIIKHQKYEHTILRVDSIAIEEKVQLLPDI
LCSANADIDTLMLDLSELLTMVMLSELVHNLDSQLANVEENYFKEFYEKFASNLQNMK
AECSNMAKCLRQKEILPSDFMFQQLPDFFDYIKINIRDISSTLGARSVFMPRDVFSSV
DSQSSKDLIDGKLRKYCNTVEKLQIALFGDKTQWHNKIGSNHATMVRSGQLTNFSKDN
KQNPNHKSSIADLDDISTSDATEVNHLWNINLLVNDITTSIIGETPEVSEELSTKTVS
KVSNLSIKLFPDTESFSSNESDSKIILQINHSRGTSVVSLMSIFLAVSGIHTLNQIFG
HCIHQKMRQSKTKQYFLALSESKKKSCIKSIKWGQLKELLEINFSSEYISQIIALPNG
LRTKFEPTSTFITVKNCNTISVSGQYFRMMVESPTQPNFWERMICINGFKVMIHIDLL
KQQMKKLNSLQNWEKLPSAITLENDSWHFSIPHHFEMFKIIDSIPTIFKSIKQMLYSL
KTSKDDLIIFPHKIETPLSLPKIKLKSKRWLFSISDDPLEAELNTIFQIGLQEQRERL
AKLQEFNKRISEDLIKSQKNAKEMKDDFEAIDNAILKHRTGLWAKDGKKRLRKSATDS
EIPLTPAALNINGKRDDRPDRTQFISPEIENAYNTLLANFSDSWIKRVKEYKVKERRE
FDKNFSFLWGFIDYTKLPKDINKKVLPFSTNPFLMNLIIENIDIDIIRPSCGIENIPN
FIHDVGKGVPKNTEYSIMIPMHLDAKFSEVRWHLRDYPLPFVSIPPLSSTQSKETIPM
RIYGDFMITEDMLQSDRELRTLFVPLIPSVTVENTDRYYSLFVPRTMTSAKIFTDLNF
EINSNHTTRVTWGGSYQPAIQQTMQCLDNFSKPPLDPSVKLGFWDKTRYLFHGKINIV
WKKRGKFEISLKGAKSPYMLGGESAGFIVGFDGNVNLKCNEDNDPKKFLSCSADKVHF
SIPNYFAKPLLVWSRPSTNTMFIPNQDDTNMQRYASFYYLLNTTSSKNEKADKEIMGK
SFIEKTGIKLSGGMTLDMGILFERLGPSLNERTFESKKHYLTRLCNPIYVQDLSKHDS
YAGFRSDFIHMSFGLSSNSNSAYNAMQLSPNGFKAFFVWWKSFSGNFPVRRGPLFGLQ
SISPKFGEHLYTISYHADVSPLFINYMYHNADADQILRKNYLEVAEFAGLKAKSSHFI
MDLHQRKEVLTEYQAGLNVRRRVMKLKFLAGDVVCQDVDIRTVSGEFSKLNYIEEKED
AEYDIFDNDMSWLDITDFQDAFFINPDNYLPKIKIMPFAFSPQFAYQKRASYGDKYQV
DPKTCKPITPFDNRVSHGCTLGHNVSLRTDLVEKRVTVLKKFREKLQEGIRKNKSAGV
SEENLNDLLSKANSSVENAELLLKDFQKIFKQHEAGQTEQPFHFDSLNLLKNTKKTLK
QFEHRFFIFNVLLKWNEDARSAIFKFFYYANLSNEFASLASGKGLREFEDVIKQREMT
DDTTSMEAIPEGTDKANTAKQCHSCDDTEFTTENLLNIFEKNITQLSCDIKNKIHHKF
FVQFITPQIQLTSLENPEACVLVSSPFFMLKTLEFDANTTSNTYMQDIFLKRHGILFG
NANAFLFNKKDYQEFFELYFGSSSYGQDKKEQWPPWLGLELGFEPSALKKKAVVRNIS
ALLHHQKLAPFSAKYDSLKDKIEDNICGYVPQVNVQVNSDEYLMLTKMALKLFLYVEP
EDEELKKYIEKLIIGYDIYDTAQTRKFVNDLHDSEQILAVVEKELLFKRSLLDDIGKL
DLSNIHNERMHELLRLYILRKVFTSNGNNYINRTLVWNIKVNETILHLLDKIGKPFLD
IAVAKLNFHRIQHTMGLRKNTVTVKMMQIFDLGENVNYHCILGPLITSSGNDTVGLAS
DVPLVQITWDVDKPVGGIKVVKNVETTLSSLTIKLEEDRLNKLFEWLSLKELIYDGNG
DEDDGASSIFDMVSSESEEGKIEFSEDISSDFNEMLKRSSDYMIVEDLKLNSFKLCIS
YKGKGKMRLANVTNFVFNFPTLRLSNQTLRVTDLLLALKKVLIKVLIKHTGRFIGNKL
KRNSKENKIADDTSPLKQLTTYNSYTEPEELR"
gene complement(992211..992284)
/locus_tag="R008_L13316"
tRNA complement(992211..992284)
/locus_tag="R008_L13316"
/product="tRNA-Ile"
/note="TI(AAU)L2"
gene 994089..995003
/locus_tag="R008_L13326"
mRNA 994089..995003
/locus_tag="R008_L13326"
/product="hypothetical protein"
CDS 994089..995003
/locus_tag="R008_L13326"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG84650.1"
/translation="MTKDIRTGDLVLCKVGSFPPWPAVVFPQRLLRNDVYRKRKSNCV
AVCFFNDPTYYWEQPSRLKELDQDSIHNFILEHSKNANQRELVNAYKEAKNFDDFNVF
LQEKFEEENRLSDLKAFEKSEGSKIVAGEDPFVGRTKVVNKRKKNSISIKEDPEDNQK
SNEEESKPNIKPSKKKRPTANSGGKSNSGNKKKVKLDYSRRVEISQLFRRRIQRNLIQ
RETPPTEHEKKETHELLNRIYENSDTKRPFFDLKALRESKLHKLLKAIVNDPDLGEFH
PLCKEILLSWADLITELKKEKLQALPTP"
gene 995528..996143
/locus_tag="R008_L13331"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene complement(996271..997230)
/gene="Nbp1"
/locus_tag="R008_L13336"
mRNA complement(996271..997230)
/gene="Nbp1"
/locus_tag="R008_L13336"
/product="Nbp1p"
CDS complement(996271..997230)
/gene="Nbp1"
/locus_tag="R008_L13336"
/note="Similar to YLR457C 'Spindle pole body (SPB)
component, required for the insertion of the duplication
plaque into the nuclear membrane during SPB duplication;
essential for bipolar spindle formation; component of the
Mps2p-Bbp1p complex'"
/codon_start=1
/product="Nbp1p"
/protein_id="EWG84651.1"
/translation="MLKSVQGLWKDFFGIRDDGRKREYGSLDEVRKRSALRSRRKQMR
PTGKSVLKRPRKVTDRKTEEKIRSNRRKTPKRRLTKIFQTIRDVFSNDNENMSKMQNV
CGDMTRILKKRSQGRPSYMDTDTAKSRILRSDAFKRKISELKYNKQRISELRSGSSDG
SSGKDRNQSLYLDREILLQRQIKKRDEKIKALESKLQSLQEALNYSNEKYRILEDLLD
SSNIDPSYTKSRRTMSNLARENDEIKPLKIDLSPSPIRRTNSLFTSSPMKTYNRDGNI
PEMQPLQENISPACPTPPYRSRETEKEDETLSPISVDFSSYLS"
gene 997794..998978
/gene="Gab1"
/locus_tag="R008_L13346"
mRNA 997794..998978
/gene="Gab1"
/locus_tag="R008_L13346"
/product="Gab1p"
CDS 997794..998978
/gene="Gab1"
/locus_tag="R008_L13346"
/note="Similar to YLR459W 'GPI transamidase subunit,
involved in attachment of glycosylphosphatidylinositol
(GPI) anchors to proteins; may have a role in recognition
of the attachment signal or of the lipid portion of GPI'"
/codon_start=1
/product="Gab1p"
/protein_id="EWG84652.1"
/translation="MDSTALKVALGCIAIRLAVNSLFPSLQQQLDQSVEFSTPVTSFR
SLQEGIYLLRNNIQVYNHGVVHHPPILIFFLSLFNSDRLISLIYALIDGLIAYQLTEV
TKAFKNLKLKVWLPGLLYAVNPLTLLSCISRSSIIFTNFAISSSLYCILAEGNVLLSS
VMISISGYLSVYPILLLIPLLGMLKSWRQRILSAIVSILSLLILLLFSYSILGSQSWS
FLTQVYGSIITFEKVFPNLGLWWYFFIEMFDTFIPFFKAVFNIFIAVFITPFTLRYHK
QPFYAFILCIGWIVLTKPYPSLGDAGFFFSFLPFFTPLFGYLRYPIISALLFLHAIVL
APIFYHLWVVLGSGNSNFFYAISLVYALAIASILVDLNWAMLRIEYDNGIPNFKLKVT
QI"
CONTIG join(APIP01000174.1:1..3897,gap(33),APIP01000175.1:1..29050,
gap(17),APIP01000176.1:1..119301,gap(529),APIP01000177.1:1..43595,
gap(15),APIP01000178.1:1..126193,gap(29),APIP01000179.1:1..107301,
gap(3217),APIP01000180.1:1..2851,gap(4041),APIP01000181.1:1..1005,
gap(560),APIP01000182.1:1..21444,gap(46),APIP01000183.1:1..43285,
gap(111),APIP01000184.1:1..36148,gap(7000),APIP01000185.1:1..5240,
gap(84),APIP01000186.1:1..13168,gap(26),APIP01000187.1:1..9017,
gap(33),APIP01000188.1:1..55144,gap(43),APIP01000189.1:1..377,
gap(22),APIP01000190.1:1..23875,gap(83),APIP01000191.1:1..21757,
gap(3382),APIP01000192.1:1..2933,gap(1131),APIP01000193.1:1..10801,
gap(41),APIP01000194.1:1..11611,gap(67),APIP01000195.1:1..27396,
gap(28),APIP01000196.1:1..24848,gap(83),APIP01000197.1:1..18409,
gap(49),APIP01000198.1:1..4314,gap(14),APIP01000199.1:1..216176)
//