LOCUS KK037031 403243 bp DNA linear CON 11-FEB-2014
DEFINITION Saccharomyces cerevisiae R008 chromosome VII genomic scaffold
scaffold11, whole genome shotgun sequence.
ACCESSION KK037031 APIP01000000
VERSION KK037031.1
DBLINK BioProject: PRJNA162713
BioSample: SAMN01915092
KEYWORDS WGS.
SOURCE Saccharomyces cerevisiae R008
ORGANISM Saccharomyces cerevisiae R008
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 403243)
AUTHORS Treu,L., Toniolo,C., Nadai,C., Sardu,A., Giacomini,A., Corich,V.
and Campanaro,S.
TITLE The impact of genomic variability on gene expression in
environmental Saccharomyces cerevisiae strains
JOURNAL Environ. Microbiol. (2013) In press
PUBMED 24238297
REMARK Publication Status: Available-Online prior to print
REFERENCE 2 (bases 1 to 403243)
AUTHORS Campanaro,S., Treu,L. and Corich,V.
TITLE Direct Submission
JOURNAL Submitted (25-FEB-2013) Department of Biology, University of Padua,
Via U. Bassi 58/b, Padova, PD 35121, Italia
COMMENT ##Genome-Assembly-Data-START##
Assembly Method :: Newbler v. 2.3
Assembly Name :: R008
Genome Coverage :: 14.0x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..403243
/organism="Saccharomyces cerevisiae R008"
/mol_type="genomic DNA"
/submitter_seqid="scaffold11"
/strain="R008"
/isolation_source="grape bunch from Raboso Piave vineyard"
/db_xref="taxon:1182966"
/chromosome="VII"
/country="Italy: Tezze di Piave (TV)"
/collection_date="Oct-2005"
gene 83..147
/locus_tag="R008_G10001"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 1464..1961
/gene="Yps5"
/locus_tag="R008_G10006"
mRNA 1464..1961
/gene="Yps5"
/locus_tag="R008_G10006"
/product="Yps5p"
CDS 1464..1961
/gene="Yps5"
/locus_tag="R008_G10006"
/note="Similar to YGL259W Protein with similarity to
GPI-anchored aspartic proteases such as Yap1p and Yap3p"
/codon_start=1
/product="Yps5p"
/protein_id="EWG86148.1"
/translation="MQLFSILSLLSSLMCSLTVLGSSASSYVKFPVQKFADIINIGTQ
DVSTVFKRNEVLNTTVINGIGVYVVKMEIGTPPQTVYLQLDTGSSDMIVNNADIAYCK
SMSDGSDYASTDNYELTATFTGPRSTTTSPELITLSALIGVNSMQETHLLLRITRLSS
MTYTY"
gene 2157..2389
/locus_tag="R008_G10011"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 4109..4729
/gene="Vel1"
/locus_tag="R008_G10021"
mRNA 4109..4729
/gene="Vel1"
/locus_tag="R008_G10021"
/product="Vel1p"
CDS 4109..4729
/gene="Vel1"
/locus_tag="R008_G10021"
/note="Similar to YGL258W 'Protein of unknown function;
highly induced in zinc-depleted conditions and has
increased expression in NAP1 deletion mutants'"
/codon_start=1
/product="Vel1p"
/protein_id="EWG86149.1"
/translation="MSFLSIFTFFSVLVSVATAVRFDLTNVTCKGLHGPHCGTYVMEV
VGQNGTFLGQSTFVGADVLTESAGDAWARYLGQETRFLPKLTTVASNETKNFSPLIFT
TNINTCNPQSIGDAMVPFANTVTGEIEYNSWADTADNASFITGLANQLFNSTDYGVQV
ASCYPSFASVILSTPTVNIFGKDDTLPDYCTAIQLKAVCPPEAGFD"
gene complement(5488..7164)
/gene="Mnt2"
/locus_tag="R008_G10036"
mRNA complement(5488..7164)
/gene="Mnt2"
/locus_tag="R008_G10036"
/product="Mnt2p"
CDS complement(5488..7164)
/gene="Mnt2"
/locus_tag="R008_G10036"
/note="Similar to YGL257C Mannosyltransferase involved in
adding the 4th and 5th mannose residues of O-linked
glycans"
/codon_start=1
/product="Mnt2p"
/protein_id="EWG86150.1"
/translation="MRRKNRLFILVVLLGIVLVVYYSQLNSLDLVEPVQSSSSGNGGC
WSYYEGLTPGWLNDFYDVNQITPNPAKDVIELVTRIKIFFNCLQQVDGHNIQRLRDIE
KKLFPYINFEKLETDESAFWHTTTRWNGEVYHASVLEFDPKNHQFLRSKPINFDTGLS
FWENWLHTVTQSGSKGIVISASDVQLNETIRLLKVLRFIKNDYPIQIVHNADLSQDSM
KSIIKYARSLDAAEYPAQELWFLNVHSLLNPKYSKKFTTYSNKWLALTFSSFETPILM
DSDTVPFVSIEKFYELKEFQKTGVLFFKDRVISDDLFESSELEILREIIYGCIGLDLE
DESKIHEQVEDPVVAQVLENMFIKKYKHHLESGLVVLHKGKHLFSMLTSIALQFSPIA
EYFHGDKDFFWLGELLSNNRFTFHPVDASNIGRLGNVVSKENTGEFYQICSVQLSHTD
RDGSLLWLNGGLNICKKASWEYDYEHRQRLNDMFQNADELREYYASPVELEGIIIPDT
SISGWINSGECFLFNYCTLFKEGEFGKLIKFKEDEKHRLSQIVDIWNKDI"
gene 7915..9312
/gene="Adh4"
/locus_tag="R008_G10041"
mRNA 7915..9312
/gene="Adh4"
/locus_tag="R008_G10041"
/product="Adh4p"
CDS 7915..9312
/gene="Adh4"
/locus_tag="R008_G10041"
/note="Similar to YGL256W 'Alcohol dehydrogenase isoenzyme
type IV, dimeric enzyme demonstrated to be zinc-dependent
despite sequence similarity to iron-activated alcohol
dehydrogenases; transcription is induced in response to
zinc deficiency'"
/codon_start=1
/product="Adh4p"
/protein_id="EWG86151.1"
/translation="MLGITYAVNSTKQLIFCCLKYLTLLGYILLSNRKKGQRTNMYKR
VISISGLLKTGVKRFSSVYCKTTINNKFTFATTNSQIRKMSSVTGFYIPPISFFGEGA
LEETADYIKSKDYKKALIVTDPGIAAIGLSGRVQKMLEERGLNVAIYDKTQPNPNIAN
VTAGLKVLKEENSEIVVSIGGGSAHDNAKAIALLATNGGEIGDYEGVNQSKKAALPLF
AINTTAGTASEMTRFTIISNEEKKIKMAIIDNNVTPAVAVNDPSTMFGLPPALTAATG
LDALTHCIEAYVSTASNPITDACALKGIDLINESLVAAYKDGKDKKARTDMCYAEYLA
GMAFNNASLGYVHALAHQLGGFYHLPHGVCNAVLLPHVQEANMQCPKAKKRLGEIALH
CGASQEDPEETIKALHVLNRTMNIPRNLKDLGVKTEDFDILAEHAMHDACHLTNPVQF
TKEQVVAIIKKAYEY"
gene 13995..15119
/gene="Zrt1"
/locus_tag="R008_G10071"
mRNA 13995..15119
/gene="Zrt1"
/locus_tag="R008_G10071"
/product="Zrt1p"
CDS 13995..15119
/gene="Zrt1"
/locus_tag="R008_G10071"
/note="Similar to YGL255W 'High-affinity zinc transporter
of the plasma membrane, responsible for the majority of
zinc uptake; transcription is induced under low-zinc
conditions by the Zap1p transcription factor'"
/codon_start=1
/product="Zrt1p"
/protein_id="EWG86152.1"
/translation="MSNVTTPWWKQWDPDTVTLTAETPDDVWKTCVLQGVYFGENEYN
GNLGARISSVFVILFVSTFFTMFPLISTKVKRLRIPLYAYLFAKYFGSGVIVATAFIH
LMDPAYGAIGGNTCVGQTGNWGLYSWCPAIMLTSLTFTFLTDLFSSVWVERKYGLSHD
HTNDEIKDTVVNNAAVVSTENENGTANGSHDTKNGTEYFEESDATSVDVVQSFQTQFY
AFLILEFGVIFHSVMIGLNLGSTGKEFATLYPVLVFHQSFEGLGIGARLSAIEFPRSK
RWWPWALCVAYGLTTPICVAIGLGVRTRYVSGSYTALVISGVLDAISAGILLYTGLVE
LLARDFIFNPHRTKNLKELSFNVICTLFGAGIMALIGKWA"
gene 15315..16214
/gene="Fzf1"
/locus_tag="R008_G10081"
mRNA 15315..16214
/gene="Fzf1"
/locus_tag="R008_G10081"
/product="Fzf1p"
CDS 15315..16214
/gene="Fzf1"
/locus_tag="R008_G10081"
/note="Similar to YGL254W Transcription factor involved in
sulfite metabolism, sole identified regulatory target is
SSU1, overexpression suppresses sulfite-sensitivity of
many unrelated mutants due to hyperactivation of SSU1,
contains five zinc fingers"
/codon_start=1
/product="Fzf1p"
/protein_id="EWG86153.1"
/translation="MTDIGRTKSRNYKCSFDGCEKVYNRPSLLQQHQNSHTNQKPYHC
DEPGCGKKFIRPCHLRVHKWTHSQIKPKACTLCQKRFVTNQQLRRHLNSHERKSKLAS
RIDRKHEGVNANVKAELDGKEGGFDPKLPSGSPMCGEEFSQGHLPGYDDMQVLQCPYK
SCQKVTSFNDDLINHMLQHHIASKLVVPSGDPSLKESLPTSEKSSSTDTTSIPQLSFS
TTGTSSSESIDSTTAQTPTDPESYWSDNRCKHSDCQELSPFASVFDLIDHYDHTHAFI
PETLVKYSYIHLYKPSVWDLFEY"
gene 16950..18410
/gene="Hxk2"
/locus_tag="R008_G10086"
mRNA 16950..18410
/gene="Hxk2"
/locus_tag="R008_G10086"
/product="Hxk2p"
CDS 16950..18410
/gene="Hxk2"
/locus_tag="R008_G10086"
/note="Similar to YGL253W 'Hexokinase isoenzyme 2 that
catalyzes phosphorylation of glucose in the cytosol;
predominant hexokinase during growth on glucose; functions
in the nucleus to repress expression of HXK1 and GLK1 and
to induce expression of its own gene'"
/codon_start=1
/product="Hxk2p"
/protein_id="EWG86154.1"
/translation="MVHLGPKKPQARKGSMADVPKELMQQIENFEKIFTVPTETLQAV
TKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLRVVLVKLGGD
RTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQFPQGISEPIPLGFTFSFP
ASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALINDTTGTLVA
SYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGKLSDDIPPSAPMAINCEYGSFDNE
HVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDMYKQGFIFKNQDLS
KFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGINTTVQERKLIRRLSELIGARA
ARLSVCGIAAICQKRGYKTGHIAADGSVYNRYPGFKEKAANALKDIYGWTQTSLDDYP
IKIVPAEDGSGAGAAVIAALAQKRIAEGKSVGIIGA"
gene complement(18730..20496)
/gene="Rtg2"
/locus_tag="R008_G10091"
mRNA complement(18730..20496)
/gene="Rtg2"
/locus_tag="R008_G10091"
/product="Rtg2p"
CDS complement(18730..20496)
/gene="Rtg2"
/locus_tag="R008_G10091"
/note="Similar to YGL252C 'Sensor of mitochondrial
dysfunction; regulates the subcellular location of Rtg1p
and Rtg3p, transcriptional activators of the retrograde
(RTG) and TOR pathways; Rtg2p is inhibited by the
phosphorylated form of Mks1p'"
/codon_start=1
/product="Rtg2p"
/protein_id="EWG86155.1"
/translation="MSTLSDSDTETEVVSRNLCGIVDIGSNGIRFSISSKAAHHARIM
PCVFKDRVGLSLYEVQYNTHTNAKCPIPRDIIKEVCSAMKRFKLICDDFGVPETSVRV
IATEATRDAINADEFVNAVYGSTGWKVEILGQEDETRVGIYGVVSSFNTVRGLYLDVA
GGSTQLSWVISSHGEVKQSSKPVSLPYGAGTLLRRMRTDDNRALFYEIKEAYKDAIEK
IGIPQEMIDDAKKEGGFDLWTRGGGLRGMGHLLLYQSEGYPIQTIINGYACTYEEFSS
MSDYLFLKQKIPGSSKEHKIFKVSDRRALQLPAVGLFMSAVFEAIPQIKAVHFSEGGV
REGSLYSLLPKEIRAQDPLLIASRPYAPLLTEKYLYLLRKSIPQEDIPEIVNERIAPA
LCNLAFVHASYPKELQPTAALHVATRGIIAGCHGLSHRARALIGIALCSRWGGNIPES
EEKYSQELEQVVLREGDKAEALRIVWWTKYIGTIMYVICGVHPGGNIRDNVFDFHVSK
RSEVETSLKELIIDDANTTKVKEESTRKNRGYEVVVRISKDDLKTSASVRSRIITLQK
KVRKLSRGSVERVKIGVQFYEE"
gene complement(20933..24648)
/gene="Hfm1"
/locus_tag="R008_G10101"
mRNA complement(join(20933..24438,24591..24648))
/gene="Hfm1"
/locus_tag="R008_G10101"
/product="Hfm1p"
CDS complement(join(20933..24438,24591..24648))
/gene="Hfm1"
/locus_tag="R008_G10101"
/note="Similar to YGL251C 'Meiosis specific DNA helicase
involved in the conversion of double-stranded breaks to
later recombination intermediates and in crossover
control; catalyzes the unwinding of Holliday junctions;
has ssDNA and dsDNA stimulated ATPase activity'"
/codon_start=1
/product="Hfm1p"
/protein_id="EWG86156.1"
/translation="MKTKFDRLGTGKRSRPSPNNIDFNDQSATFKRNKKNSRQPSFKV
GLSYNSLLDDCDDENETEEIFEGRGLQFFDKDDNFSITADDTQVTSKLFDHDLEQTPD
EEAKKPKKVTIRKSAKKCLSTTILPDSFRGVFKFTEFNKMQSEAFPSIYESNENCIIS
SPTGSGKTVLFELAILRLIKETNSDTNNTKIIYIAPTKSLCYEMYKNWFPSFVNLSVG
MLTSDTSFLETEKAKKCNIIITTPEKWDLLTRRWSDYSRLFELVKLVLVDEIHTIKEK
RGASLEVILTRMNTMCQNIRFVALSATVPNIEDLALWLKTNNELPANILSFDESYRQV
QLTKFVYGYSFNCKNDFQKDAIYNSKLIEIIEKHADNRPVLIFCPTRASTISTAKFLL
NNHIFSKSKKRCNHNPSDKILNECMQQGIAFHHAGISLEDRTAVEKEFLAGSINILCS
TSTLAVGVNLPAYLVIIKGTKSWNSSEIQEYSDLDVLQMIGRAGRPQFETHGCAVIMT
DSKMKQTYENLIHGTDVLESSLHLNLIEHLAAETSLETVYSIETAVNWLRNTFFYVRF
GKNPAAYQEVNRYVSFHSVEDSQINQFCQYLLDTLVKVKIIDISNGEYKSTAYGNAMT
RHYISFESMKQFINAKKFLSLQGILNLLATSEEFSVMRVRHNEKKLFKEINLSPLLKY
PFLTEKKQSQIIDRVSQKVSLLIQYELGGLEFPSYEGASKLHQTLVQDKFLVFRHCFR
LLKCMVDTFIEKSDGTSLKNTLFLLRSLNGHCWENTPMVLRQLKTIGLVSVRRLIRHG
ITNLEEMGHLSDTQIEYYLNLKIGNGIKIKNDISLLPCLNIRTKLENCKIENEELWLT
FKVEISATFKSSIWHGQHLSLDIETEKSSGELIDFRRLQLNKLQSPRGFRISAKISPK
LEKIEFSIHCQEIAGLGKTIVYSTDHLASQFSAKTPNIRKDLNSLEKCLFYESSSDGE
VGKTSRVSHKDGLEESLSSDDSILDYLNERKKSSKAVESAAVIHPEAHSSSHFSNGRQ
VRSNGNYECFHSCKDKTQCRHLCCKEGIPVKYIKEKGPSSIKPVSKPDQIRQPLLAKN
INTTPHLEKRLNSKPKQWQEENTDIATVHTLPSKIYNLSQQMSSMEAGEQVLKSGPEN
CPEIIPIDLESSDSYSSNTAASSISDPNGDLDFLGSDIEFE"
gene 24922..25647
/gene="Rmr1"
/locus_tag="R008_G10106"
mRNA 24922..25647
/gene="Rmr1"
/locus_tag="R008_G10106"
/product="Rmr1p"
CDS 24922..25647
/gene="Rmr1"
/locus_tag="R008_G10106"
/note="Similar to YGL250W 'Protein required for meiotic
recombination and gene conversion; null mutant displays
reduced PIS1 expression and growth defects on
non-fermentable carbon sources and minimal media;
GFP-fusion protein localizes to both cytoplasm and
nucleus'"
/codon_start=1
/product="Rmr1p"
/protein_id="EWG86157.1"
/translation="MSEEEAHKTVEVDDVGVQLDEGDEEDLLEYDDELVEEQPSDARI
RNVAETLMKSELPKVTVEYKDTTFLLFTSDDKNESNNPIICENAALYQRPMGEFMESI
RKFMGNRFGRLAFATKELVLQLKSLDLTLFEDNVYNNHISFSDVYTIFKILKERSESN
FETDIPTHLAIELSTRPRFVSRYNALVELTESSATLKNIKPFSNDETHPLIVDDNDQY
THQNTSEVIVMDIDDDVGEDSED"
gene 26110..28224
/gene="Zip2"
/locus_tag="R008_G10116"
mRNA 26110..28224
/gene="Zip2"
/locus_tag="R008_G10116"
/product="Zip2p"
CDS 26110..28224
/gene="Zip2"
/locus_tag="R008_G10116"
/note="Similar to YGL249W Meiosis-specific protein
involved in normal synaptonemal complex formation and
pairing between homologous chromosomes during meiosis"
/codon_start=1
/product="Zip2p"
/protein_id="EWG86158.1"
/translation="MIIERWEVKLSKCNQNVGGYSVLSGNLKENIKLGRRAQKYLKEL
RNLQLKPLKIGGYENCGTINGEEYFLEVIHITSGRQKIDVAVGKTWNVTNIENDIKEE
LQYELFKEKLKVEKQDMLFFSWMKSLSVQLNAPLHQKMTEHGLADDNTRLEWFNIPLL
RRSQYRKKVPYPSLRQMSSVLEVQCSTLTEEKLNFCVGFSDKPLSEWKPQIFEQTYNR
YRLQRISPEKSFKYKSRCSKYNFKTSSQSWVVKVPEHDQQLNTFEKRYDELFDAQFNK
LEFFKIRMKKLKKNKPIEKKNYKIWCLEKEDLKDLVWDPLKRICNHSRYAIFEHVTIN
REAYSIKPLRLTFQKLDSGSLDLIDNQKKTFGSIKLAMSMPDVKKTENQSIEESERHD
ETAIETQEFDENDCLSSKADINTSLAPQKRSFIDNELMSMLVTKKKIKKEKDVSDTGI
SSTSYLINSGTYANSHIEIPTSNSVYNGKEDCSFNNYSVKHSILEEDIENKCIAVNEN
KVIENQKVIQSLCKNSHLDLIEQPYFGECDFIINHSTCVYKIQASRFMQLRNNGSLHY
DKAIDDLLTEFQRVIIIVEFSEIIQDVDPDLFWKIKLYLLNSRVDVFFIHETTDFFID
WMKYFIARWAFSYNDEKEKNIANADILLDLGFNILLVRKIFQTYSLEEFFMAIIKEES
KAVKMLTVSQMTRLKKLLTLEW"
gene 28665..29774
/gene="Pde1"
/locus_tag="R008_G10126"
mRNA 28665..29774
/gene="Pde1"
/locus_tag="R008_G10126"
/product="Pde1p"
CDS 28665..29774
/gene="Pde1"
/locus_tag="R008_G10126"
/note="Similar to YGL248W 'Low-affinity cyclic AMP
phosphodiesterase, controls glucose and intracellular
acidification-induced cAMP signaling, target of the
cAMP-protein kinase A (PKA) pathway; glucose induces
transcription and inhibits translation'"
/codon_start=1
/product="Pde1p"
/protein_id="EWG86159.1"
/translation="MVVFEITILGANGGPTEYGTQCFILKPARTEDPELIAVDGGAGM
YQLREMLVQGRNENEGDDELVPSFYEHDREPIEFFIDSKLNIQKGLSKSLLQSLKRQG
EHFESANTMKKTYEVFQGITDYYITHPHLDHISGLVVNSPSIYEQENSKKKTIWGLPH
TIDVLQKHVFNDLIWPDLTAERSRKLKLKCLNPKEVQKCTIFPWDVIPFKVHHGIGVK
TGAPVYSTFYIFRDRKSKDCIIVCGDVEQDRRESEESLLEEFWSYVAKNIPLVHLKGI
LVECSCPLSSKPEQLYGHLSPIYLINELSNLNTLYNSSKGLSGLNVIVTHVKSTPAKR
DPRLTILEELRFLAEERNLGDLRISIALEGHTLFL"
gene 29945..30538
/gene="Brr6"
/locus_tag="R008_G10131"
mRNA 29945..30538
/gene="Brr6"
/locus_tag="R008_G10131"
/product="Brr6p"
CDS 29945..30538
/gene="Brr6"
/locus_tag="R008_G10131"
/note="Similar to YGL247W 'Essential nuclear envelope
integral membrane protein required for nuclear envelope
morphology, nuclear pore complex localization, nuclear
export; exhibits synthetic lethal genetic interactions
with genes involved in lipid metabolism'"
/codon_start=1
/product="Brr6p"
/protein_id="EWG86160.1"
/translation="MELRSFSRQPDGILANPRLGREEVLEGEHPQDARLARQSIWLSP
SLIAEYIQLFFNFIIGTIGLSLAIKFILMIRNDVNLKLEHNVREELDKIATCKSRYFE
NQCEPHMRVPALEVRCNEWSKCMNKEIVSGSDYQWAKAWARTLAEVINAFFEAFSIRS
FLFILISIIGIIFVTNTSFGSYRVYLNNKDTKSVRHA"
gene complement(30629..31792)
/gene="Rai1"
/locus_tag="R008_G10136"
mRNA complement(30629..31792)
/gene="Rai1"
/locus_tag="R008_G10136"
/product="Rai1p"
CDS complement(30629..31792)
/gene="Rai1"
/locus_tag="R008_G10136"
/note="Similar to YGL246C 'Nuclear protein with decapping
endonuclease activity targeted toward mRNAs with
unmethylated 7-methylguanosine cap structures; binds to
and stabilizes the exoribonuclease Rat1p; required for
pre-rRNA processing; homologous to human DOM3Z'"
/codon_start=1
/product="Rai1p"
/protein_id="EWG86161.1"
/translation="MGVSANLFVKQRGSTTALKQPKEIGFYSRTKDEEYLISDDTNLN
YYYLPDAELDRKLDLSSGFQKFKDYYKDFEDRCSLRGLLETIESSERHKGKKINADII
TFRGIARKLISCAFDSPSFNTVDLRIVSFNGQLFIKEVPEAVNAAKASSATEAGRNIN
QDLNVFTGYKFETLATLSNPLQYTPREVIEKRTKRIVSHGDEYISVVRTGVGNCKLIL
GAEVDCIFDFKENGRDNLKHYAELKCTQQVANISDTHKFERKLFRTWLQCFLVGIPRI
IYGFKDDHYVLKTVEEFSTEEVPVLLKNNNPQVGSACLEAIKWYGLLTEWLLKMIPRD
EDPHSQIRAFKLVFENNHLRLSEIEESDEEYSGLIDGEHILTNGFKEWRKSLK"
gene 32035..34161
/gene="Gus1"
/locus_tag="R008_G10141"
mRNA 32035..34161
/gene="Gus1"
/locus_tag="R008_G10141"
/product="Gus1p"
CDS 32035..34161
/gene="Gus1"
/locus_tag="R008_G10141"
/note="Similar to YGL245W 'Glutamyl-tRNA synthetase
(GluRS), forms a complex with methionyl-tRNA synthetase
(Mes1p) and Arc1p; complex formation increases the
catalytic efficiency of both tRNA synthetases and ensures
their correct localization to the cytoplasm'"
/codon_start=1
/product="Gus1p"
/protein_id="EWG86162.1"
/translation="MPSTLTINGKAPIVAYAELIAARIVNALAPNSIAIKLVDDKKAP
AAKLDDATEDVFNKITSKFAAIFDNGDKEQVAKWVNLAQKELVIKNFAKLSQSLETLD
SQLNLRTFILGGLKYSAADVACWGALRSNGMCGSIIKNKVDVNVSRWYTLLEMDPIFG
EAHDFLSKSLLELKKSANVGKKKETHKANFEIDLPDAKMGEVVTRFPPEPSGYLHIGH
AKAALLNQYFAQAYKGKLIIRFDDTNPSKEKEEFQDSILEDLDLLGIKGDRITYSSDY
FQEMYDYCVQMIKDGKAYCDDTPTEKMREERMDGVASARRDRSVEENLRIFTEEMKNG
TEEGLKNCVRAKIDYKALNKTLRDPVIYRCNLTPHHRTGSTWKIYPTYDFCVPIVDAI
EGVTHALRTIEYRDRNAQYDWMLQALRLRKVHIWDFARINFVRTLLSKRKLQWMVDKD
LVGNWDDPRFPTVRGVRRRGMTVEGLRNFVLSQGPSRNVINLEWNLIWAFNKKVIDPI
APRHTAIVNPVKIHLEGSEAPQEPKIEMKPKHKKNPAVGEKKVIYYKDIVVDKDDADV
INVDEEVTLMDWGNVIITKKNDDGSMVAKLNLEGDFKKTKHKLTWLADTKDVVPVDLV
DFDHLITKDRLEEDESFEDFLTPQTEFHTDAIADLNVKDMKIGDIIQFERKGYYRLDA
LPKDGKPYVFFTIPDGKSVNKYGAKK"
gene 34511..36196
/gene="Rtf1"
/locus_tag="R008_G10146"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 36385..37539
/gene="Tad1"
/locus_tag="R008_G10151"
mRNA 36385..37539
/gene="Tad1"
/locus_tag="R008_G10151"
/product="Tad1p"
CDS 36385..37539
/gene="Tad1"
/locus_tag="R008_G10151"
/note="Similar to YGL243W tRNA-specific adenosine
deaminase, deaminates adenosine-37 to inosine in tRNA-Ala"
/codon_start=1
/product="Tad1p"
/protein_id="EWG86163.1"
/translation="MPSEDKLGEEISTRVVNEYSKLKSACRPIIRPSGIREWTILAGV
AAINRDGGANKIEILSIATGVKALPDSELQRSEGKILHDCHAEILALRGANTVLLNRI
QNYNPSSGDKFIQHNDEIPARFNLKENWELALYISRLPCGDASMTFLNDNCKNDDFIK
IEDSDEFQYVDRSVKTILRGRLNFNRRNVVRTKPGRYDSNITLSKSCSDKLLMKQRSS
VLNCLNYELFEKPVFLKYIVIPNLEDETKHYLEQSFHTRLPNLDNEIKFLNCLKPFYD
DKLDEEDVPGLMCSVKLFMDDFSTEEAILNGVRNGFYTKSSNPLRKHCQSQVSRFAQW
ELFKKIRPEYEGISYLEFKSRQKKRSQLIIAIKNILSPDGWIPTRTDDVK"
gene complement(37684..38229)
/locus_tag="R008_G10156"
mRNA complement(37684..38229)
/locus_tag="R008_G10156"
/product="hypothetical protein"
CDS complement(37684..38229)
/locus_tag="R008_G10156"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86164.1"
/translation="MNTEGASLSEQLLDAARRNNLDLLETVFDSLDNDPEKIAKLINE
SKEPLGNTALHLCCKYGSWEVLDKILDQDGEIEIDPQNDVDGDTPLHVTVRYSQEEPE
HGTFIARNLIEVGADPTVRNYNNQKPVDLVHGDELDELIDLLQGAELAIDSTNGSGDN
NEDGEMIDDGPSDDDEEDDKK"
gene 38476..41490
/gene="Kap114"
/locus_tag="R008_G10161"
mRNA 38476..41490
/gene="Kap114"
/locus_tag="R008_G10161"
/product="Kap114p"
CDS 38476..41490
/gene="Kap114"
/locus_tag="R008_G10161"
/note="Similar to YGL241W 'Karyopherin, responsible for
nuclear import of Spt15p, Sua7p, histones H2A and H2B, and
Nap1p; amino terminus shows similarity to those of other
importins, particularly Cse1p; localization is primarily
nuclear'"
/codon_start=1
/product="Kap114p"
/protein_id="EWG86165.1"
/translation="MDINELIIGAQSADKHTREVAETQLLQWCDSDASQVFKALANVA
LQHEAFLESRQFALLSLRKLITMYWSPGFESYRSTSNVEVDVKDFIREVLLKLCLNDN
ENTKIKNGASYCIVQISAVDFPDQWPQLLTVIYDAISHQHSLNAMSLLNEIYDDVVSE
EMFFEGGIGLATMEIVFKVLNTETSTLIAKIAALKLLKACLLQMSSHNEYDEASRKNF
VSQCLATSLQILGQLLTLNFGNVDVISQLKFKSIIYENLVFIKNDFSRKHFSSELQKQ
FKIMAIQDLENVTHINANVETTESEPLLETVHDCSIYIVEFLTSVCTLQFSVEEMNKI
ITSLTILCQLSSETREIWTSDFNTFVSKETGLAASYNVRDQANEFFTSLPNPQLSLIF
KVVSSDIEHSTCNYSTLESLLYLLQCILLNDDEITGENIDQSLQILIKTLENILVSQE
IPELILARAILTIPRVLDKFIDALPDIKPLTSAFLAKSLNLALKSDKELIKSATLIAF
TYYCYFAELDSVLGPEVCSKTQEKVIRIINQVSSDAEEDTNGALMEVLSQVISYNPKE
PHSRKEILQAEFHLVFTISSEDPANVQVVVQSQECLEKLLDNINMDNYKNYIELCLPS
FINVLDSNNANNYRYSPLLSLVLEFITVFLKKKPNDGFLPDEINQYLFEPLAKVLAFS
TEDETLQLATEAFSYLIFNTDTRAIEPRLMDIMKVLERLLSLEVSDSAAMNVGPLVVA
IFTRFSKEIQPLIGRILEAVVVRLIKTQNISTEQNLLSVLCFLTCNDPKQTVDFLSSF
QIDNTDALTLVMRKWIEAFEVIRGEKRIKENIVALSNLFFLNDERLQKVVVNGDLIPY
EGDLIITRSMAKKMPDRYVQVPLYTKIIKLFVSELSFQSKQPNPEQLITSDIKQEVVN
ANKDDDNDDWEDVDDVLDYDKLKEYIDDDVDEEADDDSDDITGLMDVKESVVQLLVRF
FKEVASKDVSGFHCIYETLSDSERKVLSEALL"
gene 41644..42396
/gene="Doc1"
/locus_tag="R008_G10166"
mRNA 41644..42396
/gene="Doc1"
/locus_tag="R008_G10166"
/product="Doc1p"
CDS 41644..42396
/gene="Doc1"
/locus_tag="R008_G10166"
/note="Similar to YGL240W 'Processivity factor required
for the ubiquitination activity of the anaphase promoting
complex (APC), mediates the activity of the APC by
contributing to substrate recognition; involved in cyclin
proteolysis'"
/codon_start=1
/product="Doc1p"
/protein_id="EWG86166.1"
/translation="MDPIGINKVLDHLAPSELIKPVKSCHNKPSVLVLDDRIVDAATK
DLYVNGFQEEIQYQNPTPENLQHMFHQGIEIMDSARMINVTHLALWKPSSFKLGNPVD
FALDDNYDTFWQSDGGQPHQLDIMFSKRMDICVMAIFFSMIADESYAPSLVKVYAGHS
PSDARFYKMLEVRNVNGWVALRFLDNREDDQLLKCQFIRLLFPVNHENGKDTHLRGIR
LYVPSNEPHQDTHEWAQTLPETNNVFQDAILR"
gene 42582..45465
/gene="Cse1"
/locus_tag="R008_G10176"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(45758..46558)
/gene="Hap2"
/locus_tag="R008_G10181"
mRNA complement(45758..46558)
/gene="Hap2"
/locus_tag="R008_G10181"
/product="Hap2p"
CDS complement(45758..46558)
/gene="Hap2"
/locus_tag="R008_G10181"
/note="Similar to YGL237C 'Subunit of the heme-activated,
glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a
transcriptional activator and global regulator of
respiratory gene expression; contains sequences sufficient
for both complex assembly and DNA binding'"
/codon_start=1
/product="Hap2p"
/protein_id="EWG86167.1"
/translation="MSADETDAKFHPLETDLQSDTAAATSTAAASRSPSLRAKPIEMP
LDMGKAPSPRGEDQRVTNEEDLFLFNRLRASQNRVMDSLEPQQQSQYTSSSVSTMEPS
ADFASFSAVTTLPPPPHQQQQQQRQQQQQQQQLVVQAQYTQNQPNLQSDVLGTAIAEQ
PFYVNAKQYYRILKRRYARAKLEEKLRISRERKPYLHESRHKHAMRRPRGEGGRFLTA
AEIKAMKSKKSGASDDPDDSHEDKKITTKIIQEQPHATSTAAAADNKT"
gene complement(46819..48828)
/gene="Mto1"
/locus_tag="R008_G10186"
mRNA complement(46819..48828)
/gene="Mto1"
/locus_tag="R008_G10186"
/product="Mto1p"
CDS complement(46819..48828)
/gene="Mto1"
/locus_tag="R008_G10186"
/note="Similar to YGL236C 'Mitochondrial protein, forms a
heterodimer complex with Mss1p that performs the
5-carboxymethylaminomethyl modification of the wobble
uridine base in mitochondrial tRNAs; required for
respiration in paromomycin-resistant 15S rRNA mutants'"
/codon_start=1
/product="Mto1p"
/protein_id="EWG86168.1"
/translation="MLRVTTLASSCTSFPLQVLRRRLTISSLTSFQPTTKTQVVVIGA
GHAGCEAAAASSRTGAHTTLITPSLTDIGKCSCNPSIGGVGKGILVKEIDALDGLMGK
VTDLAGVQFKMLNRSKGPAVWGPRAQIDRELYKKYMQRELSDKKAHPNLSLLQNKVAD
LILYDPGCGHKVIKGVVLDDGTQVGADQVIITTGTFLSAEIHIGDKRIAAGRIGEQPT
YGISNTLQNEVGFQLGRLKTGTPARLAKESIDFSALEVQKGDKLPVPMSFLNETVSVE
PTKQLDCFGTHTTPQMHDFLRNNLHQSIHIQDTTIKGPRYCPSIEAKILRFPDRSSHK
IWLEPEGFNSDVIYPNGISNSMPEDVQLQMMRLIPGMANVEILQPAYGVEYDYVDPRQ
LKPSLETKLVDGLFLAGQINGTTGYEEAAAQGIIAGINAGLLSRQEREQLVLKRSQAY
IGVLIDDLINNGVIEPYRMFTSRSEFRISVRADNADFRLTPIGAQLGIISPVRLSQYS
RDKHLYDETIRALQNFKLSSQKWSSLLQANIAPQAENRSAWEIFRFKDMDLHKLYECI
PDLPINLLDIPMHVVTKINIQGKYEPYIVKQNQFVKAFQADENMLLPQDYDYRQLPTL
STECKLLLNRVQPLTIGQARRIQGITAAALFELYRVARKPSQPVM"
gene 48311..48847
/locus_tag="R008_G10191"
mRNA 48311..48847
/locus_tag="R008_G10191"
/product="hypothetical protein"
CDS 48311..48847
/locus_tag="R008_G10191"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86169.1"
/translation="MTLWPHPGSYKIKSATLFCSRDKLGCAFLSESSLCMYFLYNSLS
IWALGPHTAGPLLLLSILNCTPARSVTLPISPSRASISFTRMPLPTPPIEGLHEHLPI
SVNDGVMRVVCAPVLDDAAAASQPACPAPMTTTCVLVVGWKLVKEDMVNRLLRTCKGN
EVHEDAKVVTRSIVLWGV"
gene 49513..51921
/gene="Ade5,7"
/locus_tag="R008_G10196"
mRNA 49513..51921
/gene="Ade5,7"
/locus_tag="R008_G10196"
/product="Ade5,7p"
CDS 49513..51921
/gene="Ade5,7"
/locus_tag="R008_G10196"
/note="Similar to YGL234W Bifunctional enzyme of the 'de
novo' purine nucleotide biosynthetic pathway, contains
aminoimidazole ribotide synthetase and glycinamide
ribotide synthetase activities"
/codon_start=1
/product="Ade5,7p"
/protein_id="EWG86170.1"
/translation="MLNILVLGNGAREHVLVTKLAQSPTVGKIYVAPGNGGTATMDPS
RVINWDITPDVANFARLQSMAVEHKINLVVPGPELPLVNGITSVFHSVGIPVFGPSVK
AAQLEASKAFSKRFMSKHNIPTASYDVFTNPEEAISFLQAHTDKAFVIKADGIAAGKG
VIIPSSIDESVQAIKDIMVTKQFGEEAGKQVVIEQFLEGDEISLLTIVDGYSHFNLPV
AQDHKRIFDGDKGLNTGGMGAYAPAPVATPSLLKTIDSQIVKPTIDGMRRDGMPFVGV
LFTGMILVKDSKTNQLVPEVLEYNVRFGDPETQAVLSLLDDQTDLAQVFLAAAEHRLD
SVNIGIDDTRSAVTVVVAAGGYPESYAKGDKITLDTDKLPPHTQIFQAGTKYDSATDS
LLTNGGRVLSVTSTAQDLRTAVDTVYEAVKCVHFQNSYYRKDIAYRAFQNSESSKVAI
TYADSGVSVDNGNNLVQTIKEMVRSTRRPGADSDIGGFGGLFDLAQAGFRQNEDTLLV
GATDGVGTKLIIAQETGIHNTVGIDLVAMNVNDLVVQGAEPLFFLDYFATGALDIQVA
SDFVSGVANGCIQSGCALVGGETSEMPGMYPPGHYDTNGTAVGAVLRQDILPKINEMA
AGDVLLGLASSGVHSNGFSLVRKIIQHVALPWDAPCPWDESKTLGEGILEPTKIYVKQ
LLPSIRQRLLLGLAHITGGGLVENIPRAIPDHLQARVDMSTWEVPRVFKWFGQAGNVP
HDDILRTFNMGVGMVLIVKRENVKAVCDSLTEEGEIIWELGSLQERPKDAPGCVIENG
TKLY"
gene 52153..54885
/gene="Sec15"
/locus_tag="R008_G10206"
mRNA 52153..54885
/gene="Sec15"
/locus_tag="R008_G10206"
/product="Sec15p"
CDS 52153..54885
/gene="Sec15"
/locus_tag="R008_G10206"
/note="Similar to YGL233W 'Essential 113kDa subunit of the
exocyst complex (Sec3p, Sec5p, Sec6p, Sec8p, Sec10p,
Sec15p, Exo70p, and Exo84p), which mediates polarized
targeting of vesicles to active sites of exocytosis;
Sec15p associates with Sec4p and vesicles'"
/codon_start=1
/product="Sec15p"
/protein_id="EWG86171.1"
/translation="MDQEGQPLLSKDFQQVLLATASGNNSSWTERAVLNNESTDAVKH
EPALGQNDVFDLDPLSFDKWVPFLRRALDKNQLDPVIDELENSIEDNFQGLELQLLQD
SQMNDKLETSIDEIANIQGMVQDTLSSEISKFQIRLSESANELIVKKQMYVNNKKISL
KISEATILITKVVRILELSSKCQELITERKFFKVLQNLDSLEKLYLQEFKNYNFQFLI
EIYNSIPFLQKVTKDECINLIRNSLNLNLGKNLIKVGQEFVAIYENELLPQWLETRSK
MKLTNFKFNSPIEISMRDESFLAKLNLGEFFQLDDFHDSIMIFQNLNELSVLSGEFNK
EYELRKTKLMYPLIWKKNKTAAYQMDSLLRGTGTTPGSTAHDVSTDDPFTQSLSLHFL
QDYFLKILGFLLYDINLNKATEFILVDNNYNSTNEFWDGLMDRLSPYLSYFIDEKLKT
EEDMIKLKDFLCIYVAILENFKLNIEPLYKILVSIFEKFCSVSLRAFDDEFQILLNDD
DFMPLSINDKTLYEKVLKICWMKEGEHLSLPDPTNGEPFAVTLPFSPLYPMTCTLAKK
TYSKITAFLSIFYRHELHTLNNILVKTMDDIFNDIVNKKIRSKLESTSREEIAQILVN
LDYFIIAAKEFSNFMTRENILQNPDMEIRLSSIKYLAESRKLAETKLIELIDSKISDI
LETIEIDWQITEVRQDPDISIIDLAQFLEMMFASTLQNLPYSVQTLLIFREFDSLTRQ
FMDLLLHDTPSTITHESIMNFEVDVNYLESIIPRIFPSTPGTIDSNGYQSPMTPSTPT
FPNANGVDAPTLFENNIKSLEATFMELKQCIELLKTQGKDYNEPEIRLRKYSRIRQED
AALLLSKIQHFVSSVEGANGDDTSVMDSSSIFNSESASVIDSNTSRIAKFFNRR"
gene 55105..56032
/gene="Tan1"
/locus_tag="R008_G10211"
mRNA join(55105..55161,55220..56032)
/gene="Tan1"
/locus_tag="R008_G10211"
/product="Tan1p"
CDS join(55105..55161,55220..56032)
/gene="Tan1"
/locus_tag="R008_G10211"
/note="Similar to YGL232W Putative tRNA acetyltransferase,
RNA-binding protein required for the formation of the
modified nucleoside N(4)-acetylcytidine in serine and
leucine tRNAs but not required for the same modification
in 18S rRNA"
/codon_start=1
/product="Tan1p"
/protein_id="EWG86172.1"
/translation="MGEKRNRNGKDANSQNRKKFKVSSGFLDPGTSGIYATCSRRHER
QAAQELQLLFEEKFQELYGDIKEGEDESENDEKKDLSIEDQIKKELQELKGEETGKDL
SSGETKKKDPLAFIDLNCECVTFCKTRKPIVPEEFVLSIMKDLADPKNMVKRTRYVQK
LTPITYSCNAKMEQLIKLANLVIGPHFHDPSNVKKNYKFAVEVTRRNFNTIERMDIIN
QVVKLVNKEGSEFNHTVDLKNYDKLILVECFKSNIGMCVVDGDYKTKYRKYNVQQLYE
SKFRKDEDKSVKQ"
gene complement(56078..56650)
/locus_tag="R008_G10216"
mRNA complement(56078..56650)
/locus_tag="R008_G10216"
/product="hypothetical protein"
CDS complement(56078..56650)
/locus_tag="R008_G10216"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86173.1"
/translation="MSEQEPYEWAKHLLDTKYIEKYNIQNSNTLPSPPGFEGNSSKGN
VTRKQQDATSQTTSLAQKNQITVLQVQKAWQIALQPAKSIPMNIFMSYMSGTSLQIIP
IMTALMLLSGPIKAIFSTRSAFKPVLGNKATQSQVQTAMFMYIVFQGVLMYIGYRKLN
SMGLIPNAKGDWLPWERIAHYNNGLQWFSD"
gene complement(56802..57245)
/locus_tag="R008_G10226"
mRNA complement(56802..57245)
/locus_tag="R008_G10226"
/product="hypothetical protein"
CDS complement(56802..57245)
/locus_tag="R008_G10226"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86174.1"
/translation="MGIITLSGNVLHLLKAYPKKGLEEVSQPEPNTANDSSTEYKGKS
KDDFQMVEKSNTDERYNFTRTKKWFLLMTSEYYKLMENRLLMFCIIACSFICAIQFLF
FIIYWTNIVPRKTQRAITNLNYDYLTAHLKEQCVPYAKILDQCIL"
gene complement(57533..59988)
/gene="Sap4"
/locus_tag="R008_G10231"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 60627..62360
/gene="She10"
/locus_tag="R008_G10236"
mRNA 60627..62360
/gene="She10"
/locus_tag="R008_G10236"
/product="She10p"
CDS 60627..62360
/gene="She10"
/locus_tag="R008_G10236"
/note="Similar to YGL228W 'Putative
glycosylphosphatidylinositol (GPI)-anchored protein of
unknown function; overexpression causes growth arrest'"
/codon_start=1
/product="She10p"
/protein_id="EWG86175.1"
/translation="MGKLIKLITTLTVLVSLLQYCCEFNSGSISCERTQTLCHYTNPR
VWNTYFSRNCELYKNKVSPGFDIVARKYDTAVKPVIDDATVKVNKVAIQPAFKVIHSQ
CKKWNCGKYYQLVRSPMVKTRRFFFAKYNAFVKPNIDKFFTAEFRSHLKERILKCKNI
GHYYFTITSRCIKSKYDFIVGNTEEKLMGKFKNKDTHGIHGSVTHEPSSEDMVLTVST
MESDEEELTTTSTQTVVETITLDQEEASAVANHAHDDEASTDVEDSTDVNVNEQALLQ
EDFDMWSETILQKTQDVIQLFEKDVSKYINGKLVEEANHFKAKFQSLDDKSKKFFSKI
SLAINDIECVEGIDSETGKKIFFDKSGSTEISQYITRELVREYFNETRSTLDELTNAM
EKDLSEITDEIEKKVNAIREENVEVFEEWGDIIVNEWSKRMAYVDVINAHMGADDDTT
LDEEKAKSSVNWKKFLKGKKQIIESRDKLAHHSADLSRVNAFRQKVQKKILSFTQESG
EFLYILRSKANLQFQERERKERERKEREKAAAEEFQRQQELLRQQEEEDEEDVSYTST
STITTTTTMTL"
gene 62704..65580
/gene="Vid30"
/locus_tag="R008_G10246"
mRNA 62704..65580
/gene="Vid30"
/locus_tag="R008_G10246"
/product="Vid30p"
CDS 62704..65580
/gene="Vid30"
/locus_tag="R008_G10246"
/note="Similar to YGL227W 'Protein involved in
proteasome-dependent catabolite degradation of
fructose-1,6-bisphosphatase (FBPase); binds FBPase; shifts
the balance of nitrogen metabolism toward glutamate
production; localizes to the nucleus and the cytoplasm'"
/codon_start=1
/product="Vid30p"
/protein_id="EWG86176.1"
/translation="MSEYMDDVDREFINCLFPSYLLQQPVAYDLWILYLQHRKLFHKL
KNTNLINADENPTGVGMGRTKLTALTRKEIWSKLMNLGVLGTISFEAVNDDYLIQVYK
YFYPDVNDFTLRFGVKDSNKNSVRVMKASSDMRKNAQELLEPVLSEREMALNSNTSLE
NDRNDDDDDDDDDDDDDDDDDDDDDESDLESLEGEVDTDTDDNNEGDGSDNHEEGGEE
GSRGADADVSSAQQRAERVADPWIYQRSRSAINIETESRNLWDTSDKNSGLQYYPPDQ
SPSSSFSSPRVSSGNDKNDNEATNVLSNSGSKKKNSMIPDIYKILGYFLPSRWQAQPN
NSLQLSQDGITHLQPNPDYHSYMTYERSSASSASTRNRLRTSFENSGKVDFAVTWANK
SLPDNKLTIFYYEIKVLSVTSTESAENSNIVIGYKLVENELMEATTKKSVSRSSVAGS
SSSLGGSNNMSSNRVPSTSFTMEGTQRRDYIYEGGVSAMSLNVDGSINKCQKYGFDLN
VFGYCGFDGLITNSTEQSKEYAKPFGRDDVIGCGINFIDGSIFFTKNGIHLGNAFTDL
NDLEFVPYVALRPGNSIKTNFGLNEDFVFDIIGYQDKWKSLAYEHICRGRQMDVSIEE
FDSDESEEDETENGPEENKSTNVNEDLMDIDQEDGAAGNKDTKKLNDEKDNNLKFLLG
EDNRFIDGKLVRPDVNNINNLSVDDGSLPNTLNVMINDYLIHEGLVDVAKGFLKDLQK
DAVNVNGQHSESKDVIRHNERQIMKEERMVKIRQELRYLINKGQISKCINYIDNEIPD
LLKNNLELVFELKLANYLVMIKKSSSKDDDEIENLILKGQELSNEFIYDTKIPQSLRD
RFSGQLSNVSALLAYSNPLVEAPKEISGYLSDEYLQERLFQVSNNTILTFLHKDSECA
LENVISNTRAMLSTLLEYNAFGSTNSSDPRYYKAINFDEDVLNL"
gene complement(65782..66191)
/gene="Ost5"
/locus_tag="R008_G10251"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 66371..66742
/locus_tag="R008_G10256"
mRNA 66371..66742
/locus_tag="R008_G10256"
/product="hypothetical protein"
CDS 66371..66742
/locus_tag="R008_G10256"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86177.1"
/translation="MMGRNGILLALKRSFSTYQPPVVEITNITKLWPTLRPEVRDEIK
EYLRWRMEEDWRHIPLEETKAAYFLSYGPCGGRSKGNEWNVGYTGMRMVFNLVLFGGA
ATAFYNWKQDKKLEEQLRDLV"
gene complement(66859..66932)
/locus_tag="R008_G10261"
tRNA complement(66859..66932)
/locus_tag="R008_G10261"
/product="tRNA-Val"
/note="TV(AAC)G3"
assembly_gap 66953..72138
/estimated_length=5186
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 75349..76362
/gene="Vrg4"
/locus_tag="R008_G10271"
mRNA 75349..76362
/gene="Vrg4"
/locus_tag="R008_G10271"
/product="Vrg4p"
CDS 75349..76362
/gene="Vrg4"
/locus_tag="R008_G10271"
/note="Similar to YGL225W 'Golgi GDP-mannose transporter;
regulates Golgi function and glycosylation in Golgi'"
/codon_start=1
/product="Vrg4p"
/protein_id="EWG86178.1"
/translation="MSELKTGHAGHNPWASVANSGPISILSYCGSSILMTVTNKFVVN
LKDFNMNFVMLFVQSLVCTITLIILRILGYAKFRSLNKTDAKNWFPISFLLVLMIYTS
SKALQYLAVPIYTIFKNLTIILIAYGEVLFFGGSVTSMELSSFLLMVLSSVVATWGDQ
QAVAAKAASLAEGAAGAVASFNPGYFWMFTNCITSALFVLIMRKRIKLTNFKDFDTMF
YNNVLALPILLLFSFCVEDWSSVNLTNNFSNDSLTAMIISGVASVGISYCSGWCVRVT
SSTTYSMVGALNKLPIALSGLIFFDAPRNFLSILSIFIGFLSGIIYAVAKQKKQQAQP
LRK"
gene complement(76468..77310)
/gene="Sdt1"
/locus_tag="R008_G10276"
mRNA complement(76468..77310)
/gene="Sdt1"
/locus_tag="R008_G10276"
/product="Sdt1p"
CDS complement(76468..77310)
/gene="Sdt1"
/locus_tag="R008_G10276"
/note="Similar to YGL224C 'Pyrimidine nucleotidase;
overexpression suppresses the 6-AU sensitivity of
transcription elongation factor S-II, as well as
resistance to other pyrimidine derivatives'"
/codon_start=1
/product="Sdt1p"
/protein_id="EWG86179.1"
/translation="MTVEYTASDLATYQNEVNEQIAKNKAHLESLTHPGSKVTFPIDQ
DISATPQNPNLKVFFFDIDNCLYKSSTRIHDLMQQSILRFFQTHLKLSPEDAHVLNNS
YYKEYGLAIRGLVMFHKVNALEYNRLVDDSLPLQDILKPDIPLRNMLLRLRQSGKIDK
LWLFTNAYKNHAIRCLRLLGIADLFDGLTYCDYSRTDTLVCKPHVKAFEKAMKESGLA
RYENAYFIDDSGKNIETGIKLGMKTCIHLVENEVNEILGQTPEGAIVISDILELPHVV
PDLF"
gene complement(77546..78799)
/gene="Cog1"
/locus_tag="R008_G10281"
mRNA complement(77546..78799)
/gene="Cog1"
/locus_tag="R008_G10281"
/product="Cog1p"
CDS complement(77546..78799)
/gene="Cog1"
/locus_tag="R008_G10281"
/note="Similar to YGL223C Essential component of the
conserved oligomeric Golgi complex (Cog1p through Cog8p),
a cytosolic tethering complex that functions in protein
trafficking to mediate fusion of transport vesicles to
Golgi compartments"
/codon_start=1
/product="Cog1p"
/protein_id="EWG86180.1"
/translation="MDEVLPLFRDSHIPQIKDYQLELQNDLTKTNEAFQKNLLKNYNK
ILSLTDSVNDLSLNLKNVDQDFKSLCFNDEKFQLNKLAPLPYQTTTHISPPRDEEKVS
IPSQNILVISNWTISINNFCNRIVTSTTPSRIFDELLLNFHELSLIPVPSKFEALVKD
KCCRLQKFLVDSMKTLNLTLLQWVKLYNLLNTEFSSKWDDDLLSIFNESLFETLFNDN
VQALLISSANSKDHQYYSNQQYKDAIVVDFVNSSTFRDHLIRRTVKEINTHLDTLSTL
RAKLKEPETLHQLDIFHDNDTNLNDGTVSPLDDDALKQYIDAAVFYSKGLTNDTTLQI
YQTVQPTIEILQNLELYKCPQETLTDLRNKLITQLQEFKTQISSRLPSPLENSTSVVD
DFITSYNNHNLLQLVIDQITQLRQQ"
gene complement(79087..79623)
/gene="Edc1"
/locus_tag="R008_G10291"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(79863..80729)
/gene="Nif3"
/locus_tag="R008_G10296"
mRNA complement(79863..80729)
/gene="Nif3"
/locus_tag="R008_G10296"
/product="Nif3p"
CDS complement(79863..80729)
/gene="Nif3"
/locus_tag="R008_G10296"
/note="Similar to YGL221C 'Protein of unknown function,
similar to Listeria monocytogenes major sigma factor (rpoD
gene product); the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Nif3p"
/protein_id="EWG86181.1"
/translation="MSRAITRAQLDKLVRSITKFYPQKYADKSWDNTGLLIDCSTAQV
TTADANAKTKVLLTVDLTKSVAQEAVDANCNVIVAYHPFIFPSWNRLSPHTNPQHETA
IKLIQYGISVYCPHTAVDAARGGVNDWLVRGLNNGENVAKSYALETVSGETDDLIGYG
RFVEFNKDISLEQIVKNVKRVLRVPYVQVASLAAPSAWNQLKIKKVAVCAGSGSGVFK
QLKEDVDLYYTGEMSHHEVLKWKEMGKTVIVCNHSNTERGFLQDVMKGLLQDEGHEVV
VSKMDCDPLTVA"
gene 80811..81173
/gene="Aim15"
/locus_tag="R008_G10301"
mRNA 80811..81173
/gene="Aim15"
/locus_tag="R008_G10301"
/product="Aim15p"
CDS 80811..81173
/gene="Aim15"
/locus_tag="R008_G10301"
/note="Similar to YGL220W 'Protein involved in negative
regulation of transcription of iron regulon; forms an iron
independent complex with Fra2p, Grx3p, and Grx4p; null
mutant fails to repress iron regulon and is sensitive to
nickel'"
/codon_start=1
/product="Aim15p"
/protein_id="EWG86182.1"
/translation="MTGERIEKVKINDEFAKSHFLTTQWRETKRQRHYKMPVTEQGLR
ERIESAIPQVYHIIVTDLSYGCGQSFDIVVVSDFFQGKSKLMRSRAVNKAVKEELQEI
HAFSCKCYTEEEWSKIVV"
gene complement(81315..82694)
/gene="Mdm34"
/locus_tag="R008_G10306"
mRNA complement(81315..82694)
/gene="Mdm34"
/locus_tag="R008_G10306"
/product="Mdm34p"
CDS complement(81315..82694)
/gene="Mdm34"
/locus_tag="R008_G10306"
/note="Similar to YGL219C Mitochondrial component of the
ERMES complex that links the ER to mitochondria and may
promote inter-organellar calcium and phospholipid exchange
as well as coordinating mitochondrial DNA replication and
growth"
/codon_start=1
/product="Mdm34p"
/protein_id="EWG86183.1"
/translation="MSFRFNEAVFGDNSFNERVREKLSTALNSPSKKKLDILKSGIKV
QKVDFPTIPQLEILDLDIITQPKSLAKGICKISCKDAMLRIQTVIESNLLLINEQDTP
SFTMPQLINNGSFTIPITMTFSSIELEAITNIFVKNPGIGISFNDVDLDFKFDCSVKI
LQSTIERRLKESMHVVFKDVLPSLIFNTSQNWFTNRGESTSTIPGKREHHHQQTTMSR
NVILDGSDFQELSPINMLRLSSIVSSRSTLSLHSTVMNSLSAIPGCLERQNLYRFISR
MPSLNNYYSSQSFPQPKSSTVSSKQLVKPFYCSHNLLPKTVLDSSQYDLATITKIQSR
LFDRSNSNDDNAKPRRRKIKCKKTRTPSNLQSQGEQAVDDSTAIETVTSTPVQTPIPE
LEEQSPPYLKTTVSIRDKYVIPEKISLNLDSKKDTSKKKPFYFIGLNSQEPSNNWKWG
MEDSPPPYH"
gene 83324..85742
/gene="Kip3"
/locus_tag="R008_G10321"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 86419..87777
/gene="Clg1"
/locus_tag="R008_G10326"
mRNA 86419..87777
/gene="Clg1"
/locus_tag="R008_G10326"
/product="Clg1p"
CDS 86419..87777
/gene="Clg1"
/locus_tag="R008_G10326"
/note="Similar to YGL215W 'Cyclin-like protein that
interacts with Pho85p; has sequence similarity to G1
cyclins PCL1 and PCL2'"
/codon_start=1
/product="Clg1p"
/protein_id="EWG86184.1"
/translation="MANTFKYYPETMGNSSGYPISLPFPKGSATSAVNVARQLPKYLG
HVPSQSVHTQLPSMASLGYFNQPSSTYYAPPAPLQQHQQPPILPPPGLMYTSNNNSNV
IPPPVQMIRDGQQQPQQSNQVNGGVSENLDYDISIMSKFIMENAFVAFNANYSTDDQT
TDLFFKGISSVLNATRLPSATIFLAIDYLFKYINKLSNGIHSIGGNSINIIYQNTMIA
FILANKFNDDKTFTNSSWSQATGILINVINDFERQWLRIFNWELYDSAFLYFEFVKNF
EIFRQNQLKPAVAVPTLLSPIVNVGDTRNVNFNLKPTSTNNLLSPVSNYETPMLMPHN
MFSSPSYQSNSRSEFSSMNGYYNYYNYNQPRLNYYQQFPNIYSSPISETQFDYDFYNF
SSQQQQQQQKQHSLLPAAPQLPPPHVHNQSYGHHLGWKSMDDTINHSRFERSYFPYSA
VY"
assembly_gap 87868..88097
/estimated_length=230
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(88359..89552)
/gene="Ski8"
/locus_tag="R008_G10336"
mRNA complement(88359..89552)
/gene="Ski8"
/locus_tag="R008_G10336"
/product="Ski8p"
CDS complement(88359..89552)
/gene="Ski8"
/locus_tag="R008_G10336"
/note="Similar to YGL213C 'Ski complex component and
WD-repeat protein, mediates 3'-5' RNA degradation by the
cytoplasmic exosome; also required for meiotic
double-strand break recombination; null mutants have
superkiller phenotype'"
/codon_start=1
/product="Ski8p"
/protein_id="EWG86185.1"
/translation="MSKVFIATANAGKAHDADIFSVSACNSFTVSCSGDGYLKVWDNK
LLDNENPKDKSYSHFVHKSGLHHVDVLQTIERDAFELCLVATTSFSGDLLFYRITRED
ETKKVIFEKLDLLDSDMKKHSFWALKWGASNDRLLSHRLVATDVKGTTYIWKFHPFAD
ESNSLTLNWSPTLELQGTVESPMTPSQFATSVDISERGLIATGFNNGTVQISELSTLR
PLYNFESQHSMINNSNSIRSVKFSPQGSLLAIAHDSNSFGCITLYETEFGERIGSLSV
PTHSSQASLGEFAHSSWVMSLSFNDSGETLCSAGWDGKLRFWDVKTKERITTLNMHCD
DIEIEEDILAVDEHGDSLAEPGVFDVKFLKKGWRSGMGADLNESLCCVCLDRSIRWFR
EAGGK"
gene 89737..90687
/gene="Vam7"
/locus_tag="R008_G10341"
mRNA 89737..90687
/gene="Vam7"
/locus_tag="R008_G10341"
/product="Vam7p"
CDS 89737..90687
/gene="Vam7"
/locus_tag="R008_G10341"
/note="Similar to YGL212W 'Vacuolar SNARE protein that
functions with Vam3p in vacuolar protein trafficking; has
an N-terminal PX domain (phosphoinositide-binding module)
that binds PtdIns-3-P and mediates membrane binding;
SNAP-25 homolog'"
/codon_start=1
/product="Vam7p"
/protein_id="EWG86186.1"
/translation="MAANSVGKMSEKLRIKVDDVKINPKYVLYGVSTPNKRLYKRYSE
FWKLKTRLERDVGSTIPYDFPEKPGVLDRMWQRRYDDPEMIDERRIGLERFLNELYND
RFDSRWRDTKIAQDFLQLSKPNVSQEKSQQHLETADEVGWDEMIRDIKLDLDKESDGT
PSVRGALRARTKLHKSRERLEQDVQKKSLPSTEVTRRAALLRSLLKECDDIGTANIAQ
DRGRLLGVATSDNSSTMEVQGRTNNDLQQGQMQMVRDQEQELVALHRIIQAQRGLALE
MNEELQTQNELLTALEDDVDNTGRRLQIANKKARHFNNSA"
gene 90818..91897
/gene="Ncs6"
/locus_tag="R008_G10346"
mRNA 90818..91897
/gene="Ncs6"
/locus_tag="R008_G10346"
/product="Ncs6p"
CDS 90818..91897
/gene="Ncs6"
/locus_tag="R008_G10346"
/note="Similar to YGL211W 'Protein required for thiolation
of the uridine at the wobble position of Gln, Lys, and Glu
tRNAs; has a role in urmylation and in invasive and
pseudohyphal growth; inhibits replication of Brome mosaic
virus in S. cerevisiae'"
/codon_start=1
/product="Ncs6p"
/protein_id="EWG86187.1"
/translation="MSFTAPSDPVNKPTKVKVSQLCELCHSRKALIRRPKNLSKLCKQ
CFCLVFETEIHNTIVANNLFQRGEKVAVGASGGKDSTVLAHMLKLLNDRYDYGIEIVL
LSIDEGIIGYRDDSLATVKRNQQQYGLPLEIFSFKDLYDWTMDEIVSVAGIRNSCTYC
GVFRRQSLDRGAAKLGISHVVTGHNADDMAETVLMNILRGDVARLEKSTAIITQSSGS
PIKRSKPFKYSYQKEIVLYAHYMKLDYFSTECTYAPEAFRGTAREYMKNLEAVRPSCI
IDIIQSGENLALKAKKSNARKRVVKFVDGNRCARCGYLSSNNICKACMLLEGLEKSRA
QVAIENDTSADGAALKLRALEKLSF"
gene 92098..92766
/gene="Ypt32"
/locus_tag="R008_G10351"
mRNA 92098..92766
/gene="Ypt32"
/locus_tag="R008_G10351"
/product="Ypt32p"
CDS 92098..92766
/gene="Ypt32"
/locus_tag="R008_G10351"
/note="Similar to YGL210W 'Rab family GTPase, very similar
to Ypt31p; involved in the exocytic pathway; mediates
intra-Golgi traffic or the budding of post-Golgi vesicles
from the trans-Golgi'"
/codon_start=1
/product="Ypt32p"
/protein_id="EWG86188.1"
/translation="MSNEDYGYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKS
TIGVEFATRTIEVENKKIKAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSYE
NCNHWLTELRENADDNVAVGLIGNKSDLAHLRAVPTDEAKNFAMENQMLFTETSALNS
DNVDKAFRELIVAIFQMVSKHQVDLSGSGTNNMGSNGAPKGPTISLTPAPKEDKKKKS
SNCC"
gene 94166..95314
/gene="Mig2"
/locus_tag="R008_G10366"
mRNA 94166..95314
/gene="Mig2"
/locus_tag="R008_G10366"
/product="Mig2p"
CDS 94166..95314
/gene="Mig2"
/locus_tag="R008_G10366"
/note="Similar to YGL209W 'Protein containing zinc
fingers, involved in repression, along with Mig1p, of SUC2
(invertase) expression by high levels of glucose; binds to
Mig1p-binding sites in SUC2 promoter'"
/codon_start=1
/product="Mig2p"
/protein_id="EWG86189.1"
/translation="MPKKQTNFPVDNENRPFRCDTCHRGFHRLEHKKRHLRTHTGEKP
HHCAFPGCGKSFSRSDELKRHMRTHTGQSQRRLKKASVQKQEFLTVSGIPTIASGVMI
HQPIPQVLPANMAINVQAVNGGNIIHAPNAVHPMVIPIMAQPAPIHASAASFQPATSP
MPISTYTPVPSQSFTSFQSSIGSIQSNSDVSSIFSNMNVRVNTPRSVPNSPNDGYLHQ
QHIPQQYQHQTASPSVAKQQKTFAHSLASALSTLQKRTPVSAPSTTIESPSSPSDSSH
TSASSSAISLPFSNAPSQLAVAKELESVYLDSNRYTTKTRRERAKFEIPEEQEEDTNN
SSSSSNEEEHESLDHESSKSRKKLSGVKLPPVRNLLKQIDVFNGPKRV"
gene 95646..96893
/gene="Sip2"
/locus_tag="R008_G10371"
mRNA 95646..96893
/gene="Sip2"
/locus_tag="R008_G10371"
/product="Sip2p"
CDS 95646..96893
/gene="Sip2"
/locus_tag="R008_G10371"
/note="Similar to YGL208W 'One of three beta subunits of
the Snf1 serine/threonine protein kinase complex involved
in the response to glucose starvation; null mutants
exhibit accelerated aging; N-myristoylprotein localized to
the cytoplasm and the plasma membrane'"
/codon_start=1
/product="Sip2p"
/protein_id="EWG86190.1"
/translation="MGTTTSHPAQKKQTTKKCRAPIMSDVREKPSNAQGCEPQEMDAV
SKKVTELSLNKCSDSQDAGQPSREGSITKKKSTLLLRDEDEPTMPKLSVMETAVDTDS
GSSSTSDDEEGDIIAQTTEPKQDASPDDDRSGHSSPREEGQQQIRAKEASGGPSEIKS
SLMVPVEIRWQQGGSKVYVTGSFTKWRKMIGLIPDSDNNGSFHVKLRLLPGTHRFRFI
VDNELRVSDFLPTATDQMGNFVNYIEVRQPEKKPTNEKIRSKEAYSMRPPTSDRSSIA
LQIGKDPDDFGDGYTRFHEDLSPRPPLEYTTDIPAVFTDPSVMERYYYTLDRQQNNTD
TSWLTPPQLPPQLENVILNKYYATQDQFNENNSGALPIPNHVVLNHLVTSSIKHNTLC
VASIVRYKQKYVTQILYTPIESS"
gene 97280..100387
/gene="Spt16"
/locus_tag="R008_G10381"
mRNA 97280..100387
/gene="Spt16"
/locus_tag="R008_G10381"
/product="Spt16p"
CDS 97280..100387
/gene="Spt16"
/locus_tag="R008_G10381"
/note="Similar to YGL207W Subunit of the heterodimeric
FACT complex (Spt16p-Pob3p), which associates with
chromatin via interaction with Nhp6Ap and Nhp6Bp, and
reorganizes nucleosomes to facilitate access to DNA by RNA
and DNA polymerases"
/codon_start=1
/product="Spt16p"
/protein_id="EWG86191.1"
/translation="MEELNIDFDVFKKRIELLYSKYNEFEGSPNSLLFVLGSSNAENP
YQKTTILHNWLLSYEFPATLIALVPGKVIIITSSAKAKHLQKAIDLFKDPESKITLEL
WQRNNKEPEHNKKLFDDVIALINSAGKTVGIPEKDSYQGKFMTEWNPVWEAAVKENEF
NVIDISLGLSKVWEVKDVNEQAFLSVSSKGSDKFMDLLSNEMVRAVDEELKITNAKLS
DKIENKIDDVKFLKQLSPDLSALCPPNYKFNFDLLDWTYSPIIQSGKKFDLRVSARST
NDQLYGNGCILASCGIRYNNYCSNITRTFLIDPSEEMANNYDFLLTLQKEIVTNILKP
GRTPKEVYESVIEYIEKTKPELVPNFTKNIGSLIGLEFRDSNFILNAKNDYRKIQRGD
CFNISFGFNNLKDSQSANNYALQLADTVQIPLDETEPPRFLTNYTKAKSQISFYFNNE
EEDNNKKKSSPATKVPSKPDRNSKILRTKLRGEARGGAEDAQKEQIRKENQKKLHEKL
EKNGLLRFSAADANGPDSEPRQYFKKYESYVRDSQLPTNIRDLRIHVDWKSQTIILPI
YGRPVPFHINSYKNGSKNEEGEYTYLRLNFNSPGSSGGISKKVEELPYEESADNQFVR
SITLRSKDGDRMSETFKQIADLKKEATKREQERKALADVVQQDKLIENKTGRTKRLDQ
IFVRPNPDTKRVPSTVFIHENGIRFQSPLRTDSRIDILFSNIKNLIFQSCKGELIVVI
HIHLKNPILMGKKKIQDVQFYREASDMSVDETGGGRRGQSRFRRYGDEDELEQEQEER
RKRAALDKEFKYFADAIAEASNGLLTVENTFRDLGFQGVPNRSAVFCMPTTDCLVQLI
EPPFLVINLEEVEICILERVQFGLKNFDMVFVYKDFNKPVTHINTVPIESLDFLKQWL
TDMDIPYTVSTINLNWATIMKSLQDDPYQFFLDGGWNFLATGSDDEASDESEEEVSEY
EASEDDVSDESAFSEDEEGSEVDDDISGDESEDYTGDESEEGEDWDELEKKAARADRG
ANFRD"
gene complement(100850..105811)
/gene="Chc1"
/locus_tag="R008_G10386"
mRNA complement(100850..105811)
/gene="Chc1"
/locus_tag="R008_G10386"
/product="Chc1p"
CDS complement(100850..105811)
/gene="Chc1"
/locus_tag="R008_G10386"
/note="Similar to YGL206C 'Clathrin heavy chain, subunit
of the major coat protein involved in intracellular
protein transport and endocytosis; two heavy chains form
the clathrin triskelion structural component; the light
chain (CLC1) is thought to regulate function'"
/codon_start=1
/product="Chc1p"
/protein_id="EWG86192.1"
/translation="MSDLPIEFTELVDLTSLGISPQFLDFRSTTFESDHFVTVRETKD
GTNSVAIVDLAKGNEVTRKNMGGDSAIMHPSQMVISVRANGTIVQIFNLETKSKLKSF
TLDEPVIFWRWLSETTLGFVTARSILTSNVFDGNVNAKPQLLTLRHANLNNTQIINFV
ANKNLDWFAVVGILQENGRIAGRIQLFSKQRNISQAIDGHVAIFTNILLEGNGSTPVQ
VFVTGNRNATTGAGELRIIEIDHDASLPSQYQKKTTDIFFPPDATNDFPIAVQVSEKY
GIIYLLTKYGFIHLYELETGTNLFVNRITAESVFTAAPYNHENGIACINKKGQVLAVE
ISTSQIVPYILNKLSNVALALTVATRGGLPGADDLFQKQFESLLLQNDYQNAAKVAAS
STSLRNQNTINRLKNIQAPPGAISPILLYFSTLLDKGKLNKEETIELARPVLQQDRKQ
LFEKWLKEDKLECSEELGDIVKPFDTTLALACYLRAGAHAKVISCLAELQQFEKIIPY
CQKVGYQPNFLVLISSLIRSSPDRASEFAVSLLQNPETASQIDIEKIADLFFSQNHIQ
QGTSLLLDALKGDTPDQGHLQTRVLEVNLLHAPQVADAILGNNIFSHYDKPTIASLSE
KAGLYQRALENYTDIKDIKRCVVHTNALPIDWLVGYFGKLNVEQSLACLKALMDNNIQ
ANIQTVVQVATKFSDLIGPSTLIKLFEDYNATEGLYYYLASLVNLTEDKDVVYKYIEA
AAKMKQYREIERIVKDNNVYDPERVKNFLKDANLEDQLPLVIVCDRFDFVHEMILYLY
KSQNLKFIETYVQQVNPSKTAQVVGALLDMDCDEAFIQSLLQSVLGQVPIDELTTEVE
KRNRLKILLPFLEQSLSQGIQDQAVYNALAKIYIDSNNSPEKFLKENDQYDTLDVGHY
CEKRDPYLAYIAYEKGQNDDDLIRITNENSMYKYQARYLLERSDLDLWNKVLNQENIH
RRQLIDSVISVGIPELTDPEPVSLTVQAFMTNGLKLELIELLEKIILEPSPFNENVAL
QGLLLLSAIKYEPTKVSSYIEKLDNYDADEIAPLCIEHDLKEEAFEIYDKHEMYGKAL
KVLIEDIMSLDRAASYADKINTPELWSQIGTAQLDGLRIPDAIESYIKAEDPSNYENV
IDIAEQAGKYEELIPFLLMARKTLKEPKIDGALILAYAGLNKIHEIENLLAGSNVANL
DHVGDKLFENKGYKAARLCYSAVSNYSKLASTLVYLGDYQAAVDTARKASNIKVWKLV
NDACIEKKEFKLAQICGLNLIVHAEELDELVERYESNGYFEELISLFEAGLGLERAHM
GMFTELAILYSKYEPDKTFEHLKLFWSRINIPKVIRAVEQAHLWSELVFLYAHYDEWD
NAALTLIEKSTKDLDHAYFKEVVVKVSNLEIYYKAINFYVKFHPSLLVDLLTSLTPRL
DIPRTVKIFSKSDNLPLIKPFLINVLPKNNSVVNQAYHDLMIEEEDYKALQDAVDSYD
KFDQLGLASRLESHKLIFFKKIAALLYRRNKKWAKSLSILKEEKLWKDAIETAAISQD
PKVVEALLTYFVETGNREGFVALLYAAYNLVRIEFVLEISWMNSLEDYIKPFEISIKK
EQNDSIKKITEELAKKSGSNEEHKDGQPLMLMNSAMNVQPTGF"
gene 106466..108712
/gene="Pox1"
/locus_tag="R008_G10391"
mRNA 106466..108712
/gene="Pox1"
/locus_tag="R008_G10391"
/product="Pox1p"
CDS 106466..108712
/gene="Pox1"
/locus_tag="R008_G10391"
/note="Similar to YGL205W 'Fatty-acyl coenzyme A oxidase,
involved in the fatty acid beta-oxidation pathway;
localized to the peroxisomal matrix'"
/codon_start=1
/product="Pox1p"
/protein_id="EWG86193.1"
/translation="MTRRTTINPDSVVLNPQKFIQKERADSKIKVDQVNTFLESSPER
RTLTHALIDQIVNDPILKTDTDYYDATKLQEREITAKKIARLASYMEHDIKTVRKHFR
NTDLMKELQANDPDKASPLTNKDLFIFDKRLSLVANIDPQLGTRVGVHLGLFGNCIKG
NGTDEQIRYWLQERGATLMKGIYGCFAMTELGHGSNVAQLQTRAVYDKQNDTFIIDTP
DLTATKWWIGGAAHSATHAAVYARLIVEGKDYGVKTFVVPLRDPSTFQLLAGVSIGDI
GAKMGRDGIDNGWIQFRNVVIPREFMLSRFTKVVRSPDGSVTVKTEPQLDQISGYSAL
LSGRVNMVMDSFRFGSKFATIAVRYAVGRQQFAPRKGLSETQLIDYPLHQYRVLPQLC
VPYLVSPVAFKLMDNYYSTLDELYNASSSADKAALVTVSKKLKNLFIDSASLKATNTW
LIATLIDELRQTCGGHGYSQYNGFGKGYDDWVVQCTWEGDNNVLSLTSAKSILKKFID
SATKGRFDNTLDVDSFSYLKPQYIGSVVSGETKSSLKELGDYTEIWSITLIKLLAHIG
TLVEKSRSIDSVSKLLVLVSKFHALRCMLKTYYDKLNSRDSHISDEITKESMWNVYKL
FSLYFIDKHSGEFQQFKIFTPDQISKVVQPQLLALLPIVRKDCIGLTDSFELPDAMLN
SPIGYFDGDIYHNYFNEVCRNNPVEADGAGKPSYHALLSSMLGRGFEFDQKLGGAANA
EILSKINK"
gene complement(108932..109003)
/locus_tag="R008_G10401"
tRNA complement(108932..109003)
/locus_tag="R008_G10401"
/product="tRNA-His"
/note="TH(GUG)G1"
gene complement(110200..112413)
/gene="Kex1"
/locus_tag="R008_G10411"
mRNA complement(110200..112413)
/gene="Kex1"
/locus_tag="R008_G10411"
/product="Kex1p"
CDS complement(110200..112413)
/gene="Kex1"
/locus_tag="R008_G10411"
/note="Similar to YGL203C 'Protease involved in the
processing of killer toxin and alpha factor precursor;
cleaves Lys and Arg residues from the C-terminus of
peptides and proteins'"
/codon_start=1
/product="Kex1p"
/protein_id="EWG86194.1"
/translation="MFYNRWLGTWLAMSALIRISVSLPSSEEYKVAYELLPGLSEVPD
PSNIPQMHAGHIPLRSEDADEQDSSDLEYFFWKFTNNDSNGNVDRPLIIWLNGGPGCS
SMDGALVESGPFRVNSDGKLYLNEGSWISKGDLLFIDQPTGTGFSVEQNKDEGKIDKN
KFDEDLEDVTKHFMDFLENYFKIFPEDLTRKIILSGESYAGQYIPFFANAILNHNKFS
KIDGDTYDLKALLIGNGWIDPNTQSLSYLPFAMEKKLIDESNPNFKHLTNAHENCQNL
INSASTDEAAHFSYQECENILNLLLSYTRESSQKGTADCLNMYNFNLKDSYPSCGMNW
PKDVSFVSKFFSTPGVIDSLHLDSDKIDHWKECTNSVGTKLSNPISKPSIHLLPGLLE
SGIEIVLFNGDKDLICNNKGVLDTIDNLKWGGIKGFSDDAVSFDWIHKSKSTDDSEEF
SGYVKYDRNLTFVSVYNASHMVPFDKSLVSRGIVDIYSNDVMIIDNNGKNVMITTDDD
SDQDATTESGDKPKENLEEEEQEAQNEEGKEKEGNKDKDGDDDNDNDDDDEDDHNSEG
DDDDDDDDDDDDDDDDDEDDNNEKQSNQGLEDSRHKSSEYEQEEEEVEEFAEEISMYK
HKAVVVTIVTFLIVVLGVYAYDRRVRRKARHTILVDPNNRQHDSPNKTVSWADDLESG
LGAEDDLEQDEQLEGGAPISSTSNKAGSKLKTKKKKKYTSLPNTEIDESFEMTDF"
assembly_gap 113136..118407
/estimated_length=5272
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 118526..118621
/locus_tag="R008_G10416"
tRNA 118526..118621
/locus_tag="R008_G10416"
/product="tRNA-Lys"
/note="TK(UUU)G1"
gene 119101..120603
/gene="Aro8"
/locus_tag="R008_G10421"
mRNA 119101..120603
/gene="Aro8"
/locus_tag="R008_G10421"
/product="Aro8p"
CDS 119101..120603
/gene="Aro8"
/locus_tag="R008_G10421"
/note="Similar to YGL202W Aromatic aminotransferase I,
expression is regulated by general control of amino acid
biosynthesis"
/codon_start=1
/product="Aro8p"
/protein_id="EWG86195.1"
/translation="MTLPESRDFSYLFSDETNARKPSPLKTCIHLFQDPNIIFLGGGL
PLKDYFPWDNLSVDSPKPPFPQGIGAPIDEQNCIKYTVNKDYADKSANPSNDIPLSRA
LQYGFSAGQPELLNFIRDHTKIIHDLKYKDWDVLATAGNTNAWESTLRVFCNRGDVIL
VEAHSFSSSLASAEAQGVITFPVPIDADGIIPEKLAKVMENWTPGAPKPKLLYTIPTG
QNPTGTSIADHRKEAIYKIAQKYDFLIVEDEPYYFLQMNPYIKDLKEREKAQSSPKQD
HDEFLKSLANTFLSLDTEGRVIRMDSFSKVLAPGTRLGWITGSSKILKPYLSLHEMTI
QAPAGFTQVLVNATLSRWGQKGYLDWLLGLRHEYTLKRDCAIDALYKYLPQSDAFVIN
PPIAGMFFTVNIDASVHPEFKTKYNSDPYQLEQSLYHKVVERGVLVVPGSWFKSEGET
EPPQPAESKEVSNPNIIFFRGTYAAVSPEKLTEGLKRLGDTLYEEFGISK"
gene complement(120896..123940)
/gene="Mcm6"
/locus_tag="R008_G10426"
mRNA complement(120896..123940)
/gene="Mcm6"
/locus_tag="R008_G10426"
/product="Mcm6p"
CDS complement(120896..123940)
/gene="Mcm6"
/locus_tag="R008_G10426"
/note="Similar to YGL201C 'Protein involved in DNA
replication; component of the Mcm2-7 hexameric complex
that binds chromatin as a part of the pre-replicative
complex; forms a subcomplex with Mcm4p and Mcm7p'"
/codon_start=1
/product="Mcm6p"
/protein_id="EWG86196.1"
/translation="MSSPFPADTPSSNRPSNSSPPPSSIGAGFGSSSGLDSQIGSRLH
FPSSSQPHVSNSQTGPFVNDSTQFSSQRLQTDGSATNDMEGNEPARSFKSRALNHVKK
VDDVTGEKVREAFEQFLEDFSVQSTDTGEVEKVYRAQIEFMKIYDLNTIYIDYQHLSM
RENGALAMAISEQYYRFLPFLQKGLRRVVRKYAPELLNTSDSLKRSEGDEGQADDDEQ
QDDDMNGSSLPRDSGSSAAPGNGTSAMATRSITTSTSPEQTERVFQISFFNLPTVHRI
RDIRSEKIGSLLSISGTVTRTSEVRPELYKASFTCDMCRAIVDNVEQSFKYTEPTFCP
NPSCENRAFWTLNVTRSRFLDWQKVRIQENANEIPTGSMPRTLDVILRGDSVERAKPG
DRCKFTGVEIVVPDVTQLGLPGVKPSSTLDTRGISKTTEGLNSGVTGLRSLGVRDLTY
KISFLACHVISIGSNIGASSPDANSNNRETELQMAANLQANNVYQDNERDQEVFLNSL
SSDEINELKEMVKDEHIYDKLVRSIAPAVFGHEAVKKGILLQMLGGVHKSTVEGIKLR
GDINICVVGDPSTSKSQFLKYVVGFAPRSVYTSGKASSAAGLTAAVVRDEEGGDYTIE
AGALMLADNGICCIDEFDKMDISDQVAIHEAMEQQTISIAKAGIHATLNARTSILAAA
NPVGGRYNRKLSLRGNLNMTAPIMSRFDLFFVILDDCNEKIDTELASHIVDLHMKRDE
AIEPPFSAEQLRRYIKYARTFKPILTKEARSYLVEKYKELRKDDAQGFSRSSYRITVR
QLESMIRLSEAIARANCVDEITPSFIAEAYDLLRQSIIRVDVDDVEMDEEFDNIESQS
HAASGNNDDGTGSGVITSEPPADIEEGQSEATARPGTSEKKKTTVTYDKYVSMMNMIV
RKIAEVDREGAEELTAVDIVDWYLLQKENDLGSLAEYWEERRLAFKVIKRLVKDRILM
EIHGTRHNLRDLENEENENNKTVYVIHPNCEVLDQLEPQDSS"
gene 125303..125375
/locus_tag="R008_G10436"
tRNA 125303..125375
/locus_tag="R008_G10436"
/product="tRNA-Lys"
/note="TK(CUU)G1"
gene complement(125727..126338)
/gene="Emp24"
/locus_tag="R008_G10441"
mRNA complement(125727..126338)
/gene="Emp24"
/locus_tag="R008_G10441"
/product="Emp24p"
CDS complement(125727..126338)
/gene="Emp24"
/locus_tag="R008_G10441"
/note="Similar to YGL200C Integral membrane component of
endoplasmic reticulum-derived COPII-coated vesicles, which
function in ER to Golgi transport"
/codon_start=1
/product="Emp24p"
/protein_id="EWG86197.1"
/translation="MASFATKFVIACFLFFSASAHNVLLPAYGRRCFFEDLSKGDELS
ISFQFGDRNPQSSSQLTGDFIIYGPERHEVLKTVRDTSHGEITLSAPYKGHFQYCFLN
ENTGIETKDVTFNIHGVVYVDLDDPNTNTLDSAVRKLSKLTREVKDEQSYIVIRERTH
RNTAESTNDRVKWWSIFQLGVVIANSLFQIYYLRRFFEVTSLV"
gene 126624..127331
/gene="Yip4"
/locus_tag="R008_G10451"
mRNA 126624..127331
/gene="Yip4"
/locus_tag="R008_G10451"
/product="Yip4p"
CDS 126624..127331
/gene="Yip4"
/locus_tag="R008_G10451"
/note="Similar to YGL198W 'Protein that interacts with Rab
GTPases, localized to late Golgi vesicles; computational
analysis of large-scale protein-protein interaction data
suggests a possible role in vesicle-mediated transport'"
/codon_start=1
/product="Yip4p"
/protein_id="EWG86198.1"
/translation="MSYGREDTTIEPDFIEPDAPLAASGGVADNIGGTMQNSGSRGTL
DETVLQTLKRDVVEINSRLKQVVYPHFPSFFSPSDDGIGAADNDISANCDLWAPLAFI
ILYSLFVSHARSLFSSLFVSSWFILLVMALHLRLTKPHQRVSLISYISISGYCLFPQV
LNALVSQILLPLAYHIGKQNRWIVRVLSLVKLVVMALCLMWSVAAVSWVTKSKTIIEI
YPLALCLFGMAWLSTIL"
gene 127730..132193
/gene="Mds3"
/locus_tag="R008_G10456"
mRNA 127730..132193
/gene="Mds3"
/locus_tag="R008_G10456"
/product="Mds3p"
CDS 127730..132193
/gene="Mds3"
/locus_tag="R008_G10456"
/note="Similar to YGL197W 'Putative component of the TOR
regulatory pathway; negative regulator of early meiotic
gene expression; required, with Pmd1p, for growth under
alkaline conditions; has an N-terminal kelch-like domain'"
/codon_start=1
/product="Mds3p"
/protein_id="EWG86199.1"
/translation="MPLLQPSACFCYPLKLPPLPLASDSNEFDECARKRLTLDYRTGS
AVTLTRSNIFVHGGLTIPLNLPVVNSMQLQKELILFFAKEKNNGSSFRNLNEWISKET
FFLDLMSRTWYRVKTSFDQRTEELLKAESSSAKADNDTNEIKTDIKKGKSLESPLKER
LFHSLCYLDGCLYIFGGLTVSPQSGYELIATNELWKLDLNTKKWSLLSDDPQIARRFN
HTMHVKNENNDNRDTKLIIVGGLNNMDQPVKKIDIYNISQNCWQSETIPKQPMEITTN
VNGIPLALSKDQNFSILVENNEANVPTLAFYMRSDQIDEYLGKYPSKIKENSPIVALP
LLSESQGIRMPSNPALPKKLLNVPYELLAPTGDYFGFNIIIGGFHPNYQSSNFHCFIY
DINSGKWSRVATACPDCDINKHRFWRVFVWKSHHQTILLGTKTDDYYSPSVQRFDHLS
TFGLPLVNIFNKTIQLPHHRISASSLPIPIENFAKHKDTPLKKVSFTSSATSQFENYI
RYIAPPLEMSSIQSMFPPYAMVLGKDALEIYGKPLSDFEFITSEGDSIGIPCYLLRKR
WGRYFDMLLSQSYTKVCADYETTGTQSTLIKFSPHSSRNSSKAVRQEGRLSSSGSLDN
YFEKNFPIFARTSVSEAQNTQPQVANADAKAPNTPSTSDEPSSSSSSDLYSTPHYQRN
NDEEDDEDPVSPKPVSKSNSIYRPIRKTESSSTTSSSNGMIFRVPFKEKAAVTSNTEA
LLESNLSLQELSRRRSSLMSIPSGELLRSSISEAEHQRRASHPLTSSPLFEDSGTPCG
KQLQQLQQHTIQNPHNHLSPRRFSRSARSSISYVSSSSDRRGNSISSRSTSDSFGTPP
VLGVLNVPLPPQTREPNEPPPPCPAMSTCSNTRRSNTLTDYMHSNKASPFSSRRSSHI
GRRSSTPETENAFSATPRASLDGQMLGKSLKEGSTSQYTQPRMNSFPKANETIQTPTS
SNNEWSRQSVTSNTDSFDSLQSNFALELEPLLTPRSLYMPWPTSTVRAFAEFFYTGQV
NSKWLLAPVALDLLVMAKIYEIPLLYKLILEVLYSILAKKEESLSLICTSLMETFRTK
TLNSYKGDEEKTNTYLTSNDNYQELLKLKVSLENIDNGYYDPDLLRKQSRAQSSSTQE
SSGSANGEKTATGAGSLETSSTNVPTVFAGGPRDSHNSVGSIGFPNSMNIQGSRRSTS
GFSPRVKMKSSLSKEIDPKTFYEEYEPKEGKSFDDNDDQQTNIGSFNLHLFDMNYGSI
SSSSTNSISSSDLEEKEEQGQLQDLLEIEREDSAEILDARFRNKEDDKVTKDISKDKK
RNYLPHEKNNLKAKEGKETRDVREEEEEFDFGLGMLSLNKIKREAKHVDKVDDSVDPL
FKSSAFPQSPIRAYGSTTRSSSASGKPFRDNRSFNAFSVLTLENMASANALPPVDYVI
KSIYRTTVLVNDIRLMVRCMDCMELSKNLRALKKKTMEDISKLKGICKPSP"
gene 132915..134201
/gene="Dsd1"
/locus_tag="R008_G10461"
mRNA 132915..134201
/gene="Dsd1"
/locus_tag="R008_G10461"
/product="Dsd1p"
CDS 132915..134201
/gene="Dsd1"
/locus_tag="R008_G10461"
/note="Similar to YGL196W 'D-serine dehydratase (aka
D-serine ammonia-lyase); converts D-serine to pyruvate and
ammonia by a reaction dependent on pyridoxal 5'-phosphate
and zinc; may play a role in D-serine detoxification;
L-serine is not a substrate'"
/codon_start=1
/product="Dsd1p"
/protein_id="EWG86200.1"
/translation="MSDVLSQYKGCSVRDLPTPNFVINEEKFDKNCTTMLNNVEKLSQ
ECGVPIKFRAHVKTHKTAKGTLKQLGHGLPLAKRTTRAILVSTLKEAEELLNYQDRQC
SDYIDDITYSLPCCVPEFIPLLSNLSRRVNNFQVFVDNIEHLENLKNFGRPASGKKWS
VFIKVDMGTKRAGLAFDSPEFLSLLKKLTSSEIKEVIEPYGFYAHAGHSYSSTSINDT
QNLLMEEVKAVNSAAKVLCSVDPQFDPSKLTLSVGATPTSNSLKLDNKSTLVKFVTTQ
LVSTLEIHCGNYCMYDLQQVATGCVQDHELSGFVLGTVLSSYPSRGELLSNTGVMCLT
REASSIKGFGICADLEHVLKSESFSREWYVARVSQEHGILRPIRNWNETTALKLGSKI
AVLPQHACITMGQFPYYFVVNSQGIVNDVWLPFQKW"
gene 134557..142575
/gene="Gcn1"
/locus_tag="R008_G10466"
mRNA 134557..142575
/gene="Gcn1"
/locus_tag="R008_G10466"
/product="Gcn1p"
CDS 134557..142575
/gene="Gcn1"
/locus_tag="R008_G10466"
/note="Similar to YGL195W 'Positive regulator of the Gcn2p
kinase activity, forms a complex with Gcn20p; proposed to
stimulate Gcn2p activation by an uncharged tRNA'"
/codon_start=1
/product="Gcn1p"
/protein_id="EWG86201.1"
/translation="MTAILNWEDISPVLEKGTRESHVSKRVPFLQDISQLVRQETLEK
PQLSEIAFVLLNTFPIYEDNRSKSLVTSVLLDILNLEPCLLENFIRFISDVVISNPAT
KAVADYLNLLDWINSFLIFVSHNSNLFEEYIPKLLVVHSYATFGVETILDNQEEGKKS
QDKQNQHRKRIRYCIFQATVKAFLKCLKDNDDSISFMKISIKTVLESYSKLKITSVGV
VMIMGALTQAALQLLSRQPALHSVLKENSAEKYCEYLGKEVFLGKNPPSSFCLEIGLK
PFLKEFVSQELFIKFFIPNIEKAVLRSPEVGFSILSELYAGVSSEKVNLLNAFTSSKL
INQYFSSFKSSKEVVRSVSLQSMIILLRKISNTDTTLEDLTKLIDEIFKNIKSNLNAD
YKSLISKILIEIPLTHYEVSEKTCKGLSPYIGKEGNEAALTLMLNAFFVHYFSLGKPI
EDLDKIISAGFADKKPALKKCWFAAFLNNSNAASEEVILNFIDGCLEFVKDSIIHYQT
HGHACILASIEFTNKILALDNTELNDRVMQLIETLPENSSIGDAILTAALSTELSIEN
RIHAVNLLQELFYKKPEFIGFSVIDAIERRMRVQELIPQQNTSFKYVTSVLLAITSEL
PDKEASIKVLINALVIAQWNIFNIKNGWAGLVLRARLDPAEVVKEHASVIMEKILEIT
GSCEWIDTIYGACGLQAAAYAAFIQPTEFTPILCKTIEADLTADDFSRLSEEDFEIFA
GEEGVLVVDVLEESMNKKLSNKNSKEYETLMWEQKIRKEQAKKNVKKLSKEEQELVNE
QLAKESAVRSHVSEISTRLKRGIRLVSELSKAACLVQNGIAIWFPLAVTKLLYLCSEP
NISKLTEDVNIVFLQLSQNVSERLGNIRLFLGLATLRVHNANGISQDYLQEPLVELLT
RVLFRIKFVSNQAAIDPISLTYILPLLINVLEKGKAIALKNADKPVVKAEFVEEDEEE
EHLLLAMEIISVHAEAFEDPSIPRISIVEVLLSLLSLPSKAKIAKDCFNALCQSISVA
PNQEDLDMILSNLLSPNQFVRSTILETLDNEFELEPFMKYSPEVFICRFDSDPSNREI
ADFIWEFNKFVVNDELLKSIFPLFNQDDSGLRLFAANAYAFGAVSLFTSEENSSKDYL
NDLLNFYKERAKPLEPILDQFGLVLVSASEQKDPWQGRSTVAITLKIMAKAFSAEDDT
VVNIIKFLVDDGGLVDREPIVRQEMKEAGVELITLHGSQNSKDLIPIFEEALSSSTDS
ALKENVIILYGTLARHLQQSDARIHTIIERLLSTLDTPSADIQQAVSACIAPLVFQFK
QKVGDYLGILMEKLLNPTVASSMRKGAAWGIAGLVKGYGISALSEFDIIRNLIEAAED
KKEPKRRESVGFCFQYLSESLGKFFEPYVIEILPNILKNLGDAVPEVRDATARATKAI
MAHTTGYGVKKLIPVAVSNLDEIAWRTKRGSVQLLGNMAYLDPTQLSASLSTIVPEIV
GVLNDSHKEVRKAADESLKRFGEVIRNPEIQKLVPVLLQAIGDPTKYTEEALDSLIQT
QFVHYIDGPSLALIIHIIHRGMHDRSANIKRKACKIVGNMAILVDTKDLIPYLQQLID
EVEIAMVDPVPNTRATAARALGALVERLGEEQFPDLIPRLLDTLSDESKSGDRLGSAQ
ALAEVISGLGLTKLDEMLPTILAGVTNFRAYIREGFMPLLLFLPVCFGSQFAPYINQI
IQPILSGLADNDENIRDTALKAGKLIVKNYATKAVDLLLPELERGMFDENDRIRLSSV
QLTGELLFQVTGISSRNEFSEEDGDHNGEFSGKLVDVLGQDRRDRILAALFVCRNDTS
GIVRATTVDIWKALVPNTPRAVKEILPTLTGMIVTHLASSSNVLRNIAAQTLGDLVRR
VGGNALSQLLPSLEESLIETSNSDSRQGVCIALYELIESASTETISQFQSTIVNIIRT
ALIDESATVREAAALSFDVFQDVVGKTAVDEVLPYLLHMLESSDNSDFALLGLQEIMS
KKSDVIFPILIPTLLAPPIDAFRASALGSLAEVAGSALYKRLSIIINALVDAIIGTSE
DESTKGALELALDRVFLSVNDDEGLHPLLQQIMSLLKSDNIEKRIAVLERLPNFFDKT
VLDFDVYIPNFVSHAILSLDDEDQRVVNGNFNALSTLLKKVDKPTLEKLVKPAKQSLA
LTGRQGQDVAAFKLPRGPNCILPIFLHGLMYGSNDEREESALAIADVVSKTPAANLKP
FVSVITGPLIRVVGERFSSDIKAAILFALNVLFIKIPMFLRPFIPQLQRTFVKSLSDA
TNETLRLRAAKALGALIEHQPRVDPLVIELVTGAKQATDEGVKTAMLKALLEVIMKAG
SKLNENSKTNIVNLVEEEMLGSNDKLAVAYAKLIGSLSEILSNDEAHKILQDKVLNAD
LDGETGKFAILTLNSFLKDAPTHIFNTGLIDEFVSYILNAIRSPDVYFGENGTIAAGK
LLLLEGEKRSPFVKKDAAEPFKIGDENINLLINELSKAVLQPASNSTDVRRLALVVIR
TLARFKFDECIKQFFDVVGPSVFSCLRDPVIPIKLAAEKAYLALFKLVEEDDMHTFNE
WFAEISDRGSSIETVTGTTIQLRSVGDYTKRVGKRLANVERERIAAGGDAETMFSDRF
EDEREIWAVGGVELTTDA"
gene complement(142751..142993)
/locus_tag="R008_G10471"
mRNA complement(142751..142993)
/locus_tag="R008_G10471"
/product="hypothetical protein"
CDS complement(142751..142993)
/locus_tag="R008_G10471"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86202.1"
/translation="MSNKEITCIKPFKIIALTLLIVLIINLSYKLFLRRYLKSSVIWC
LGIANTDRNDIMWWQVSPLLERWVWQLVDNYESGYE"
gene complement(143411..144778)
/gene="Hos2"
/locus_tag="R008_G10476"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(144968..145277)
/locus_tag="R008_G10481"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 145296..147098
/gene="Ime4"
/locus_tag="R008_G10486"
mRNA 145296..147098
/gene="Ime4"
/locus_tag="R008_G10486"
/product="Ime4p"
CDS 145296..147098
/gene="Ime4"
/locus_tag="R008_G10486"
/note="Similar to YGL192W 'Probable mRNA N6-adenosine
methyltransferase required for entry into meiosis;
transcribed in diploid cells; haploids repress IME4
transcription via production of antisense IME4
transcripts; antisense transcription is repressed in
diploids'"
/codon_start=1
/product="Ime4p"
/protein_id="EWG86203.1"
/translation="MINDKLVHFLIQNYDDILRAPLSGQLKDVYSLYISGGYDDEMQK
LRNGKDDVLQFEQFWNDLQDIIFATPQSIQFDQNLLVADRPEKIVYLDVFSLKILYNK
FHAFYYTLKSSSSSCEEKVSSSTTKPGADSEKDQLLGRLLGVLNWDVNVSNQGLPREQ
LSNRLQNLLREKPSSFQLAKERAKYTTEVIEYIPICSDYSHASLLSTSVYIVNNKIVS
LQWSKISACQENHPGLIECIQSKIHFIPNIKPQTDISLGDCSYLDTCHKLNTCRYIHY
LQYIPSCLQERADRETASENKRIRSNVSIPFYTLGNCSAHCIKKALPAQWIRCDVRKF
DFRVLGKFSVVIADPAWNIHMNLPYGTCNDIELLGLPLHELQDEGIIFLWVTGRAIEL
GKESLNNWGYNVINEVSWIKTNQLGRTIVTGRTGHWLNHSKEHLLVGLKGNPKWINKH
IDVDLIVSMTRETSRKPDELYGIAERLAGTHARKLEIFGRDHNTRPGWFTIGNQLTGN
CIYEMDVERKYQEFMKSKTGTSHTGTKKIDKKQPSKLQQQHQQQYWNNMDMGSGKYYA
EAKQNPMNQKHTPFESKQQQKQQFQTLNNLYFAQ"
gene 147858..148247
/gene="Cox13"
/locus_tag="R008_G10491"
mRNA 147858..148247
/gene="Cox13"
/locus_tag="R008_G10491"
/product="Cox13p"
CDS 147858..148247
/gene="Cox13"
/locus_tag="R008_G10491"
/note="Similar to YGL191W 'Subunit VIa of cytochrome c
oxidase, which is the terminal member of the mitochondrial
inner membrane electron transport chain; not essential for
cytochrome c oxidase activity but may modulate activity in
response to ATP'"
/codon_start=1
/product="Cox13p"
/protein_id="EWG86204.1"
/translation="MFRQCAKRYASSLPPNALKPAFGPPDKVAAQKFKESLMATEKHA
KDTSNMWVKISVWVALPAIALTAVNTYFVEKEHAEHREHLKHVPDSEWPRDYEFMNIR
SKPFFWGDGDKTLFWNPVVNRHIEHDD"
gene complement(148855..150435)
/gene="Cdc55"
/locus_tag="R008_G10496"
mRNA complement(148855..150435)
/gene="Cdc55"
/locus_tag="R008_G10496"
/product="Cdc55p"
CDS complement(148855..150435)
/gene="Cdc55"
/locus_tag="R008_G10496"
/note="Similar to YGL190C 'Non-essential regulatory
subunit B of protein phosphatase 2A (PP2A), which has
multiple roles in mitosis and protein biosynthesis;
involved in regulation of mitotic exit; found in the
nucleus of most cells, also at bud neck and bud tip'"
/codon_start=1
/product="Cdc55p"
/protein_id="EWG86205.1"
/translation="MAQNNFDFKFSQCFGDKADIVVTEADLITAVEFDYTGNYLATGD
KGGRVVLFERSNSRHCEYKFLTEFQSHDAEFDYLKSLEIEEKINEIKWLRPTQRSHFL
LSTNDKTIKLWKVYEKNIKLVSQNNLTEGVTFAKKGKPDNHNSRGGSVRAVLSLQSLK
LPQLSQHDKIIAATPKRIYSNAHTYHINSISLNSDQETFLSADDLRINLWNLDIPDQS
FNIVDIKPTNMEELTEVITSAEFHPQECNLFMYSSSKGTIKLCDMRQNSLCDNKTKTF
EEYLDPINHNFFTEITSSISDIKFSPNGRYIASRDYLTVKIWDVNMDNKPLKTINIHE
QLKERLSDTYENDAIFDKFEVNFSGDSSSVMTGSYNNNFMIYPNVVTSGDNDNGIVKT
FDEHNAPNSNSNKNIHNSIQNKDSSSSGNSHKRRSNGRNTGMVGSSNSSRSSIAGGEG
ANPEDSGTEMNEIVLQADKTAFRNKRYGSLAQRSARNKDWGDDIDFKKNILHFSWHPR
ENSIAVAATNNLFIFSAL"
gene complement(151272..151631)
/gene="Rps26a"
/locus_tag="R008_G10501"
mRNA complement(151272..151631)
/gene="Rps26a"
/locus_tag="R008_G10501"
/product="Rps26ap"
CDS complement(151272..151631)
/gene="Rps26a"
/locus_tag="R008_G10501"
/note="Similar to YGL189C 'Protein component of the small
(40S) ribosomal subunit; nearly identical to Rps26Bp and
has similarity to rat S26 ribosomal protein'"
/codon_start=1
/product="Rps26ap"
/protein_id="EWG86206.1"
/translation="MPKKRASNGRNKKGRGHVKPVRCVNCSKSIPKDKAIKRMAIRNI
VEAAAVRDLSEASVYPEYALPKTYNKLHYCVSCAIHARIVRVRSREDRKNRAPPQRPR
FNRENKVSPADAAKKAL"
gene complement(151867..152007)
/locus_tag="R008_G10506"
mRNA complement(151867..152007)
/locus_tag="R008_G10506"
/product="hypothetical protein"
CDS complement(151867..152007)
/locus_tag="R008_G10506"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86207.1"
/translation="MLTTQKCESREGKNDEIFELGESNSDKILLKHGKCNLFSERKPV
NH"
gene complement(152747..153214)
/gene="Cox4"
/locus_tag="R008_G10521"
mRNA complement(152747..153214)
/gene="Cox4"
/locus_tag="R008_G10521"
/product="Cox4p"
CDS complement(152747..153214)
/gene="Cox4"
/locus_tag="R008_G10521"
/note="Similar to YGL187C 'Subunit IV of cytochrome c
oxidase, the terminal member of the mitochondrial inner
membrane electron transport chain; precursor N-terminal 25
residues are cleaved during mitochondrial import;
phosphorylated; spermidine enhances translation'"
/codon_start=1
/product="Cox4p"
/protein_id="EWG86208.1"
/translation="MLSLRQSIRFFKPATRTLCSSRYLLQQKPVVKTAQNLAEVNGPE
TLIGPGAKEGTVPTDLDQETGLARLELLGKLEGIDVFDTKPLDSSRKGTMKDPIIIES
YDDYRYVGCTGSPAGSHTIMWLKPTVNEVARCWECGSVYKLNPVGVPNDDHHH"
gene complement(154080..155819)
/gene="Tpn1"
/locus_tag="R008_G10526"
mRNA complement(154080..155819)
/gene="Tpn1"
/locus_tag="R008_G10526"
/product="Tpn1p"
CDS complement(154080..155819)
/gene="Tpn1"
/locus_tag="R008_G10526"
/note="Similar to YGL186C 'Plasma membrane pyridoxine
(vitamin B6) transporter; member of the purine-cytosine
permease subfamily within the major facilitator
superfamily; proton symporter with similarity to Fcy21p,
Fcy2p, and Fcy22p'"
/codon_start=1
/product="Tpn1p"
/protein_id="EWG86209.1"
/translation="MNRDNMDTTKRKEDHTKHTTDVIEFYEEGTAASSLNIATEKANS
SPSILRRIINRAAWLSKKVDAMGVESTGIQRISPYERGTSKKQFLHVAGLWLSATGGL
SSMSSFLLGPLLFGLSFRESVASSLISVTIGCLIAAYCSIMGPQSGCRQMVTARYLFG
WWFVKLVALASIIGVMGWSVVNSVVGGEMLAAISNDKVPLWVGIVIVTVCSFLVAIFG
IKQVIKVETYLSVPVLTAFLLLYISSSDKYSFVNAYVSKGNLDSSTRKGNWMSFFSLC
YSITATWGSITADYYILFPEDTPYIQIFCLTFFGTFLPTCFVGILGLLLASVAMSYKP
WSVEYDSHGMGGLLWAGFQRWNGFGKFCVVVLVFSLVSNNIINTYSAAFSIQLSSVFC
AKIPRWFWSIVCTIICLVCALIGRNHFSTILGNFLPMIGYWISMYFILLFEENLVFRR
FFLHLYTKEFPTVTGEINGPELVGSSKEVEKDAVTNIHLLKRKHKVTKHRYNWDKWED
YEVLTHGYAATFAFIVGVAGVVVGMAQAYWIGPIAAKFGEYGGDVAMWLSMAFSGVVY
PPCRYLELRKFGR"
gene complement(156210..157349)
/locus_tag="R008_G10531"
mRNA complement(156210..157349)
/locus_tag="R008_G10531"
/product="hypothetical protein"
CDS complement(156210..157349)
/locus_tag="R008_G10531"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86210.1"
/translation="MCDSPATTGKPTILFIADPCETSATLNSKAFKEKFRILRYQLDT
KEAFLNFLERHEQDKICAIYAGFPAFKKIGGMTRSIIEHKSFPRKNLKCIVLCSRGYD
GWDLDTLRKHEIRLYNYQDDENEKLIDDLKLHQVGNDVADCALWHILEGFRKFSYYQK
LSRETGNTLTARAKAAEKSGFAFGHELGNMFAESPRGKKCLILGLGSIGKQVAYKLQY
GLGMEIHYCKRSEDCTMSQNESWKFHLLDETIYAKLYQFHAIVVTLPGTPQTEHLINR
KFLEHCNPGLILVNLGRGKILDLRAVSDALVTGRINHLGLDVFNKEPEIDEKIRSSDR
LTSITPHLGSATKDVFEQSCELALTRILRVVSGEAASDEHFSRVV"
gene complement(157657..159054)
/gene="Str3"
/locus_tag="R008_G10536"
mRNA complement(157657..159054)
/gene="Str3"
/locus_tag="R008_G10536"
/product="Str3p"
CDS complement(157657..159054)
/gene="Str3"
/locus_tag="R008_G10536"
/note="Similar to YGL184C 'Peroxisomal cystathionine
beta-lyase, converts cystathionine into homocysteine; may
be redox regulated by Gto1p'"
/codon_start=1
/product="Str3p"
/protein_id="EWG86211.1"
/translation="MPIKRLDTVVVNTGSQNDQHSASVPPVYLSTTFKVDLNNEDAQN
YDYSRSGNPTRSVLQHQIGKLYRVPQENVLAVSSGMTALDVILRGLVLLNGTDNHTPT
IIAGDDLYGGTQRLLNFFKQQSHAVSVHVDTSDFEKFKTVFQSLDKVDCVLLESPTNP
LCKVVDIPRILRFVKCISPDTTVVVDNTMMSGLNCNPLQLNPGCDVVYESATKYLNGH
HDLMGGVIISKTPEIASKLYFVINSTGAGLSPMDSWLLVRGLKTLGVRLYQQQRNAMI
LAHWLENSCGFKPTRTNKATKTRFVGLRSNPDFKLHKSFNNGPGAVLSFETGSFEHSK
RLVSSKKLSIWAVTVSFGCVNSLLSMPCKMSHASIDPELRKERDFPEDLVRLCCGIEN
IVDLKKDLLAAMVDADIIEVRENGKYLFNKLNKNLAVNTTIDDLHKPLSIYEEFYNQD
LIRKDSELNIKSSKL"
gene complement(159569..160311)
/gene="Mnd1"
/locus_tag="R008_G10541"
mRNA complement(join(159569..160225,160309..160311))
/gene="Mnd1"
/locus_tag="R008_G10541"
/product="Mnd1p"
CDS complement(join(159569..160225,160309..160311))
/gene="Mnd1"
/locus_tag="R008_G10541"
/note="Similar to YGL183C 'Protein required for
recombination and meiotic nuclear division; forms a
complex with Hop2p, which is involved in chromosome
pairing and repair of meiotic double-strand breaks'"
/codon_start=1
/product="Mnd1p"
/protein_id="EWG86212.1"
/translation="MGPKRQTVSLQEKKNRILNFFQETYTFYNIKELEKSIPKKCGIS
PMIVKDLVQQMIDEDGVISVEKCGNINIYWCFKNQTLQKLYDSSELIKKKIQEVKCDI
ATYKQELDKTLATGRRKKFTVGQKSYNREALLEKRKKIQDEIKKKSNSLQKIESIRWD
AAKIQENKQQIRLKKVHLEKTTDNIEILIDYLYKKFFLKPEQIRKEFGIPEEFKEFTE
V"
gene 160932..162140
/gene="Gts1"
/locus_tag="R008_G10551"
mRNA 160932..162140
/gene="Gts1"
/locus_tag="R008_G10551"
/product="Gts1p"
CDS 160932..162140
/gene="Gts1"
/locus_tag="R008_G10551"
/note="Similar to YGL181W 'Arf3p GTPase Activating Protein
(GAP) that localizes to endocytic patches; gts1 mutations
affect budding, cell size, heat tolerance, sporulation,
life span, ultradian rhythms; localizes to nucleus and
induces flocculation when overexpressed'"
/codon_start=1
/product="Gts1p"
/protein_id="EWG86213.1"
/translation="MRFRSSSHSLKHVDRELKDLINSSENANKCGECGNFYPTWCSVN
LGVFLCGRCASVHRKVFGSRDDDAFSNVKSLSMDRWTREDIDELVSLGGNKGNARFWN
PKNVPFPFDGDDDKAIVEHYIRDKYILGKFRYDEIKPEDFGSRMDDFDGESDRFDERN
RSRSRSRSHSFYKRGHNRSDYGGSRDSFQSSGSRYSRQLAELKDMGFGDTNKNLDALS
SAHGNINRAIDYLEKSSSSRNSVSAAATTSTPPLPRRRATTSGPQPAIFDGTNVITPD
FTSNSASFVQAKPAVFDGTLQQYYDPATGMIYVDQQQYAMAMQQQQQQQQQLAVAQAQ
AQAQAQAQVQAQAQAQAQAQAQAQQIQMQQLQMQQQQQAPLSFQQQAPLSFQQMSQGG
NLPQGYFYTQ"
gene 163109..165802
/gene="Atg1"
/locus_tag="R008_G10561"
mRNA 163109..165802
/gene="Atg1"
/locus_tag="R008_G10561"
/product="Atg1p"
CDS 163109..165802
/gene="Atg1"
/locus_tag="R008_G10561"
/note="Similar to YGL180W 'Protein ser/thr kinase required
for vesicle formation in autophagy and the
cytoplasm-to-vacuole targeting (Cvt) pathway; structurally
required for phagophore assembly site formation; during
autophagy forms a complex with Atg13p and Atg17p'"
/codon_start=1
/product="Atg1p"
/protein_id="EWG86214.1"
/translation="MGDIKNKDHTTSVNHNLMASAGNYTAEKEIGKGSFATVYRGHLT
SDKSQHVAIKEVSRAKLKNKKLLENLEIEIAILKKIKHPHIVGLIDCERTSTDFYLIM
EYCALGDLTFLLKRRKELMENHPLLRTVFEKYPPPSENHNGLHRAFVLSYLQQLASAL
KFLRSKNLVHRDIKPQNLLLSTPLIGYHDSKSFHELGFVGIYNLPILKIADFGFARFL
PNTSLAETLCGSPLYMAPEILNYQKYNAKADLWSVGTVVFEMCCGTPPFRASNHLELF
KKIKRANDVITFPSYCNIEPELKELICSLLTFDPAQRIGFEEFFANKVVNEDLSSYEL
EDDLPELESKSKGIVESNMFVSEYLSKQPKSPNSNLAGHQSMADNPAELSDALKNSNI
LTAPAVKTDHTQAVDKKASNNKYHNSLVSDRSFEREYVVVEKKSVEVNSLADEVAQAG
FNPNPIKHPTSTQNQNVLLNEQFSPNNQQYFQNQGENPRLLRATSSSSGGSDGSRRPS
LVDRRLSISSLNPSNALSRALGIASTRLFGGANQQQQQQQITSSPPYSQTLLNSQLFH
ELTENIILRIDHLQHPETLKLDNTNIVSILESLAAKAFVVYSYAEVKFSQIVPLSTTL
KGMANFENRRSMDSNAIAEEQDSDDAEEEDETLKKYKEDCLSTKTFGKGRTLSATSQL
SATFNKLPRSEMILLCNEAIVLYMKALSILSKSMQVTSNWWYESQEKSCSLRVNVLVQ
WLREKFNECLEKADFLRLKINDLRFKHASEVAENQTLEEKGSSEEPVYLEKLLYDRAL
EISKMAAHMELKGENLYNCELAYATSLWMLETSLDDDDFTNAYGDYPFKTNIHLKSND
VEDKEKYHSVLDENDRIIIRKYIDSIANRLKILRQKMNHQN"
gene complement(166452..168134)
/gene="Tos3"
/locus_tag="R008_G10566"
mRNA complement(166452..168134)
/gene="Tos3"
/locus_tag="R008_G10566"
/product="Tos3p"
CDS complement(166452..168134)
/gene="Tos3"
/locus_tag="R008_G10566"
/note="Similar to YGL179C 'Protein kinase, related to and
functionally redundant with Elm1p and Sak1p for the
phosphorylation and activation of Snf1p; functionally
orthologous to LKB1, a mammalian kinase associated with
Peutz-Jeghers cancer-susceptibility syndrome'"
/codon_start=1
/product="Tos3p"
/protein_id="EWG86215.1"
/translation="MVLLKEPVQPLPRSSLLYNNASNSSSRIKETRKVKLLYNPLTKR
QILNNFEILATLGNGQYGKVKLARDLGTGALVAIKILNRFEKRSGYSLQLKVENPRVN
QEIEVMKRCHHENVVELYEILNDPESTKVYLVLEYCSRGPVKWCPENKMEIKAVGPSI
LTFQQSRKVVLDVVSGLEYLHSQGITHRDIKPSNLLISSNGTVKISDFGVAMSTATGS
TNIQSSHEQLLKSRALGTPAFFAPELCSTEKEYSCSSAIDIWSLGVTIYCLLFGKLPF
NANSGLELFDSIINKPLEFPSYEEMLNGATSGITMEEYTDAKDLLKKLLQKDPDKRIK
LADIKVHPFMCHYGKSDAASVSTNLETFHELKVSPPSSCKRVELVSLPVNSSFASLDS
VYMENFDHNNLRTGADRNSTYSPSIYDANTLSPSAYHNIGSRESSYSSFSSFTSSTAF
ASQISIQDAPAIGDQQCLIGESGSSLRVNSCEFPQYTTMSPVGEYPFESTEASLSSTL
TPVGNVPQRIKAHLVEGKSNSKDDLRIEADASLVFEASDAQRTRRRMSLYKL"
gene 170401..173617
/gene="Mpt5"
/locus_tag="R008_G10576"
mRNA join(170401..170403,171041..173617)
/gene="Mpt5"
/locus_tag="R008_G10576"
/product="Mpt5p"
CDS join(170401..170403,171041..173617)
/gene="Mpt5"
/locus_tag="R008_G10576"
/note="Similar to YGL178W 'Member of the Puf family of
RNA-binding proteins; binds to mRNAs encoding chromatin
modifiers and spindle pole body components; involved in
longevity, maintenance of cell wall integrity, and
sensitivity to and recovery from pheromone arrest'"
/codon_start=1
/product="Mpt5p"
/protein_id="EWG86216.1"
/translation="MINNEPFPSADSASILTTSTSNNSLMSYNHQPQLSINSVQSLLE
PVTPPPLGQMNNKRNHQKAHSLDLSGFNQFISSTQSPLALMNNTSTSNSANSFSPNPN
AASNSTGLSASMANPPAILPLINEFDLEMDGPRRKSSHDFTVVAPSNSGVNTSSLIME
TPSSSVTPAASLRNFSNSNNAASKCGVDNSSFGLSSSTSSSMVEISALPLRDLDYIKL
ATDQFGCRFLQKKLETPSESNMVRDLMYEQIKPFFLDLILDPFGNYLVQKLCDYLTAK
QKTLLIQTIYPNVFQISINQYGTRSLQKIIDTVDNEVQIDLIIKGFSQEFTSIEQVVT
LINDLNGNHVIQKCIFKFSPSKFGFIIDAIVEQNNIITISTHKHGCCVLQKLLSVCTL
QQIFKISVKIVQFLPGLINDQFGNYIIQFLLDIKELDFYLLAELFNRLSNELCQLSCL
KFSSNVVEKFIKKLFRIITGFIVNNNGGASQRTAVASDDVINASMNILLTTIDIFTVN
LNVLIRDNFGNYALQTLLDVKNYSPLLAYNKNGNAIGQNSSSTLNYGNFCNDFSLKIG
NLIVLTKELLPSIKTTSYAKKIKLKVKAYAEATGIPFTDISPQVTAMSHNNLQTINNE
NKNPHNKNSHNHNHNHNHNHAHNNNNNNNQKSHTRHFSLPANAYHRRSNSSVTNNFSN
QYAQDQKIHSPQQIMNFNQNAYPSMGAPSFNSQTNPPLVSHNSLQNFDNRQFANLMAH
PNSAAPIHSFSSSNITNVNPNVSRGFKQPGFMMNETDKINANHFSPYSNANSQNFNES
FVPRMQYQTEGANWDSSLSMKSQHIGQGPYNQVNMSRNASISNMPAMNTARTSDELQF
TLP"
gene complement(174482..176146)
/locus_tag="R008_G10586"
mRNA complement(174482..176146)
/locus_tag="R008_G10586"
/product="hypothetical protein"
CDS complement(174482..176146)
/locus_tag="R008_G10586"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86217.1"
/translation="MTPNLPGFYYDRERRRYFRISENQSISTTGTTNQYRKDNIKRQC
VEENYDKKFSMIKKKRQQTLQKYKLSLLNPLERAFRPLSYEKYMIGLNMQYASHSLTE
GHHSHSSANVKSLNFPHRIQIGVLANCILLVTQEGCFHSKLVFATNKGYVAGFSSLDN
FPEENFFTGFSMAELNPMLKYKSEPTDVFKTMKLERTVAIKEGPSHYFYHNVNTRSNV
HTFAIFIQDFSSLKLLKIRQVKLKENCQVHDSLVVGDTLIITVNYRCHFYDLIPETFP
NPYIFSPAKSSRKHKSRSDITSLSFCLQEDALSPLKKTNTGVFYLGYRNGDSMAIVFT
NITNMTLQYSKTNGMASESRNQPIRNSPKSVVSIKALNNKGLMLISGMADKEDVQQLV
IADTFLEDILTEIPVVSFKTKFLNVTKDTEILEISDDGRYFIYGSTSARDGKGDFEVF
CTTLSGNLDYEKSEGGNITLYPIGGMKNYCRLENFQFESIHLHSAFIPPRYVKLFDAV
EPLGEESSTSPYDIPEEGLSQKICILIRREDDPYNGANIFITSALT"
gene complement(176352..177389)
/gene="Sae2"
/locus_tag="R008_G10591"
mRNA complement(176352..177389)
/gene="Sae2"
/locus_tag="R008_G10591"
/product="Sae2p"
CDS complement(176352..177389)
/gene="Sae2"
/locus_tag="R008_G10591"
/note="Similar to YGL175C 'Endonuclease that processes
hairpin DNA structures with the MRX complex; involved in
meiotic and mitotic double-strand break repair;
phosphorylated in response to DNA damage and required for
normal resistance to DNA-damaging agents'"
/codon_start=1
/product="Sae2p"
/protein_id="EWG86218.1"
/translation="MVTGEENVYLKSSLSILKELSLDELLNVQYDVTTLIAKRVQALQ
NRNKCVLEEPNSKLAEILCHEKNAPQQSSQTSAGPGEQDSEDFILTQFDEDIKKESAE
VHYRNENKHTVQLPLVTMPPNRHKRKISEFSSPLNGLNNLSDLEDCSDTVIHEIDNDK
ENKTRKLLGIELENPGSTSPNLYKNVKDNFLFDFNTNPLTKRAWILEDFRPNEDIAPV
KRGRRKLERFYAQVGKPEDSKHRSLSVVIESQNSDYEFAFDNLRNRSKSPPGFGRLDF
PSTQEGNEDKKKSQEIIRRKTKYRFLMASNNKIPPYEREYVFKREQLNQIVDDGCFFW
SDKLLQIYARC"
gene 177612..178412
/gene="Bud13"
/locus_tag="R008_G10596"
mRNA 177612..178412
/gene="Bud13"
/locus_tag="R008_G10596"
/product="Bud13p"
CDS 177612..178412
/gene="Bud13"
/locus_tag="R008_G10596"
/note="Similar to YGL174W 'Subunit of the RES complex,
which is required for nuclear pre-mRNA retention and
splicing; involved in bud-site selection; diploid mutants
display a unipolar budding pattern instead of the
wild-type bipolar pattern'"
/codon_start=1
/product="Bud13p"
/protein_id="EWG86219.1"
/translation="MALHQYLSETYGPTKPKNKTKKKKKESKSDANSDKTSLIVKERL
STLQQEQEKSGVASFSKFDKQKSKNIWKNLETNELSHAITHPSASSIAGNESKNDLKE
IRAQEPLVTVADKSKTRKTIYRDAQGHKIQEDSKIDDSSFSQSKYEDEKAAEREQYLK
NLNMGDVQKLGINVDAHDKKKNQTASSLTIEDPAITFTHDKERTVKTSLLGRKLYDKP
APENRFAIMPGSRWDGVHRSNGFEEKWFAKQNEINEKKVQSYTLQEDY"
gene complement(178594..183180)
/gene="Kem1"
/locus_tag="R008_G10601"
mRNA complement(178594..183180)
/gene="Kem1"
/locus_tag="R008_G10601"
/product="Kem1p"
CDS complement(178594..183180)
/gene="Kem1"
/locus_tag="R008_G10601"
/note="Similar to YGL173C 'Evolutionarily-conserved 5'-3'
exonuclease component of cytoplasmic processing (P) bodies
involved in mRNA decay; plays a role in
microtubule-mediated processes, filamentous growth,
ribosomal RNA maturation, and telomere maintenance'"
/codon_start=1
/product="Kem1p"
/protein_id="EWG86220.1"
/translation="MGIPKFFRYISERWPMILQLIEGTQIPEFDNLYLDMNSILHNCT
HGNDDDVTKRLTEEEVFAKICTYIDHLFQTIKPKKIFYMAIDGVAPRAKMNQQRARRF
RTAMDAEKALKKAIENGDEIPKGEPFDSNSITPGTEFMAKLTKNLQYFIHDKISNDSK
WREVQIIFSGHEVPGEGEHKIMNFIRHLKSQKDFNQNTRHCIYGLDADLIMLGLSTHG
PHFALLREEVTFGRRNSEKKSLEHQNFYLLHLSLLREYMELEFKEIADEMQFEYNFER
ILDDFILVMFVIGNDFLPNLPDLHLNKGAFPVLLQTFKEALLHTDGYINEHGKINLKR
LGVWLNYLSQFELLNFEKDDIDVEWFNKQLENISLEGERKRQRVGKKLLVKQQKKLIG
SIKPWLMEQLQEKLSPDLPDEEIPTLELPKDLDMKDHLEFLKEFAFDLGLFITHSKSK
GSYSLKMDLDSINPDETEEEFQNRVNSIRKTIKKYQNAIIVEDKEELETEKTIYNERF
ERWKHEYYHDKLKFTTDSEEKVRDLAKDYVEGLQWVLYYYYRGCPSWSWYYPHHYAPR
ISDLVKGLDQDIEFDLSKPFTPFQQLMAVLPERSKNLIPPTFRPLMYDEQSPIHDFYP
AEVQLDKNGKTADWEAVVLISFVDEKRLIEAMQPYLCKLSPEEKTRNQFGKDLIYSFN
PQVDNIYKSPLGGIFSDIEHNHCVEKEYITIPLDSSEIRYGLLPNAKLGAEMLAGFPT
LLSLPFTSSLEYNETMVFQQPSKQQSMVLQITDIYKTNNVTLEDFSKRHLNKVIYTRW
PYLRESKLVSLTDGKTIYEYQESNDKKKFRFITKPAETQDKKLFNSLKNSMLRMYAKQ
KAVKIGPVEAIATVFPVTGLVRDSDGGYIKTFSPTPDYYPLQLVVESVVNEDERYKER
GPIPIEEEFPLNSKVIFLGDYAYGGETTIDGYSSDRRLKITVEKKFLDSEPTIGKERL
QMDHQAVKYYPSYIVAKNMHLHPLFLSKITSKFMITDATGKHINVGIPVKFEARHQKV
LGYARRNPRGWEYSNLTLNLLKEYRQTFPDFFFRLSKVGNDIPVLEDLFPDTSTKDAM
NLLDGIKQWLKYVSSKFIAVSLESDSLTKTSIAAVEDHIMKYAANIEGHERKQLAKVP
REAVLNPRSSFALLRSQKFDLGDRVVYIQDSGKVPIFSKGTVVGYTTLSSSLSIQVLF
DHEIVAGNNFGGRLRTNRGLGLDASFLLNITNRQFIYHSKASKKALEKKKQSNNRNNN
TKTAHKTPSKQQSEEKLRKERAHDLLNFIKKDTNEKNSESVDNKSMGSQKDSKPAKKV
LLKRPAQKSSENVQVGLANFEKAPLDNPTVAGSIFNAVANQYSDGIGSNLNIPTPPHP
MNVVGGPIPGANDVADVGLPYNIPPGFMTHPNGLHPLHPHQMPYPNMNGMSIPPPAPH
GFGQPIPFPPPPPMTNVSDQGSRIVVNEKESQDLKKFINGKQHSNGSTIGGETKNSRK
GEIKPSSGTNSTECQSPKSQSNAADRDNKKDEST"
gene 183769..185187
/gene="Nup49"
/locus_tag="R008_G10606"
mRNA 183769..185187
/gene="Nup49"
/locus_tag="R008_G10606"
/product="Nup49p"
CDS 183769..185187
/gene="Nup49"
/locus_tag="R008_G10606"
/note="Similar to YGL172W Subunit of the
Nsp1p-Nup57p-Nup49p-Nic96p subcomplex of the nuclear pore
complex (NPC), required for nuclear export of ribosomes"
/codon_start=1
/product="Nup49p"
/protein_id="EWG86221.1"
/translation="MFGLNKASSTPAGGLFGQASGASTGNANTGFSFGGTQTGQNTGP
STGGLFGAKPAGSTGGLGASFGQQQQQSQTNAFGGSATTGGGLFGNKPNNTANTGGGL
SGANSNSNSGGLFGSNNAQTSGGLFGNNNTNNINNSSSGMNNASTGLFGSKPAGGTSL
FGNTSTSSAPAQNQGMFGAKPAGTSLFGNNAGNTTTGGGLFGSKPTGATSLFGSSNNN
NNNNNSNNIMSASGGLFGNQQQQLQQQPQMQCALQNLSQLPITPMTRISELPPQIRQE
IEQLDQYIQKQVQISHHLKADTIDHDELIDSIPRDVAYLLKSESATSQYLKQDLKKIS
SFKSLIDEDLLDTQTFSVLLQQLLTPGSKISSNDLDKFFQKKIHLYEKKLEDYCRILS
DIETAVNGIDTDLFGAPNNPNSTAITADLGSSEAENLLQLKTGLAAIVSTVIEEFTLF
MDIAERIAVLHQKTKTLASLSI"
gene 185459..187153
/gene="Rok1"
/locus_tag="R008_G10611"
mRNA 185459..187153
/gene="Rok1"
/locus_tag="R008_G10611"
/product="Rok1p"
CDS 185459..187153
/gene="Rok1"
/locus_tag="R008_G10611"
/note="Similar to YGL171W 'ATP-dependent RNA helicase of
the DEAD box family; required for 18S rRNA synthesis'"
/codon_start=1
/product="Rok1p"
/protein_id="EWG86222.1"
/translation="MDIFRVLTRGASVKKESGPKAKAADYSVINGKDENHKEDNNESQ
IVKELDFFRNKRIISKVEDDREKTTENDSPNKEEKSGNDDGLIKPVITNTVEASALRK
SYKGNVSGIDIPLPIGSFEDLISRFSFDRRLLNNLIENGFTEPTPIQCECIPVALNNR
DVLACGPTGSGKTLAFLIPLVQQIIDDKQTAGLKGLIISPTKELANQIFIECFKLSHK
IFLEKKRPLQVALLSKSLGAKLKNKVVSDKKYDIIISTPLRLIDVVKNEALDLSKVKH
LIFDEADKLFDKTFVEQSDDILSACREPSLRKAMFSATIPSNVEEIAQSIMMDPVRVI
IGHKEAANTNIEQKLIFCGNEEGKLIAIRQLVQEGEFKPPIIIFLESITRAKALYHEL
MYDRINVDVIHAERTALQRDRIIERFKTGELWCLICTDVLARGIDFKGVNLVINYDVP
GSSQAYVHRIGRTGRGGRSGKAITFYTKQDSVAIKPIINVMKQSGCEVSEWMDKMAKM
TRKEKESIKNGKAHKERKQITTVPKMDKAKRRRQQEMIAASKRRKNEELSKKHFSK"
gene complement(187221..188462)
/gene="Spo74"
/locus_tag="R008_G10616"
mRNA complement(187221..188462)
/gene="Spo74"
/locus_tag="R008_G10616"
/product="Spo74p"
CDS complement(187221..188462)
/gene="Spo74"
/locus_tag="R008_G10616"
/note="Similar to YGL170C Component of the meiotic outer
plaque of the spindle pole body, involved in modifying the
meiotic outer plaque that is required prior to prospore
membrane formation"
/codon_start=1
/product="Spo74p"
/protein_id="EWG86223.1"
/translation="MGAGTLLNGLEKENFPNNIHSDLPAYPNMDSQEDGNTSEESKRN
SPVKQKSPKDEEKSSKMGTASNIFHENKDIHERSEHTDDFNDGLKLAPDSSPSLKECQ
FKNWESFWCNTEGYKTKHMQPFHFTSGLEEIKEPVMELNISTSPYKGQRPNSAPTEYS
AATTAFTKTQLEVSFLKTNLLTYIKKEIDICLSSVPFFDDAVQMQKKFLEYRDIDLDE
EYELKILGELLNDLNFFHMQENSLLNRELAVRRFSNQPESQNLPSIRDFRNPLLPINN
RPSPPLGLKRNGKSFEETYDFTSNTSNFWGEKAELQNSITGGTPYFFHPNNIHQTKPF
MSFENQNELLFQRKNSDYKQHFNSGRNIHNGVESKSYRGVGLNDSYQKGYAAMTKSFG
NIDLNRMPRRSNEEMYSWSRN"
gene complement(188782..188854)
/locus_tag="R008_G10621"
tRNA complement(188782..188854)
/locus_tag="R008_G10621"
/product="tRNA-Lys"
/note="TK(CUU)G2"
gene 189126..190406
/gene="Sua5"
/locus_tag="R008_G10626"
mRNA 189126..190406
/gene="Sua5"
/locus_tag="R008_G10626"
/product="Sua5p"
CDS 189126..190406
/gene="Sua5"
/locus_tag="R008_G10626"
/note="Similar to YGL169W 'Single-stranded telomeric
DNA-binding protein, required for normal telomere length;
null mutant lacks N6-threonylcarbamoyl adenosine (t6A)
modification in the anticodon loop of ANN-decoding tRNA;
member of conserved YrdC/Sua5 family'"
/codon_start=1
/product="Sua5p"
/protein_id="EWG86224.1"
/translation="MYLGRHFLAMTSKALFDTKILKVNPLSIIFSPDAHIDGSLPTIT
DPETEAALVEAARIIRDTDETVAFPTETVYGLGGSALNDNSVLSIYKAKNRPSDNPLI
THVSSIDQLNRKVFNQPHLSGTSLFDNIPSIYRPLISSLWPGPLTILLPVPSSEHSAL
SKLTTADQPTFAVRIPANPVARALIALSDTPIAAPSANASTRPSPTLASHVYHDLKDK
IPIILDGGACKVGVESTVVDGLCNPPMLLRPGGFTYEEIVKLGGEAWSLCKVENKKTV
EKGEKVRTPGMKYRHYSPSAKVVLLVPHCEGDGILKGVDRMERLKRLIETELKANRNI
KKIAILTSLKLRDSDLQSKIFNEPDFSSKTFIIERLGQSGEEIQTNLFAALRKVDEND
KVDLIFVEGINEEGEGLAVMNRLRKAAANNCIQF"
gene 190498..190830
/gene="Hur1"
/locus_tag="R008_G10631"
mRNA 190498..190830
/gene="Hur1"
/locus_tag="R008_G10631"
/product="Hur1p"
CDS 190498..190830
/gene="Hur1"
/locus_tag="R008_G10631"
/note="Similar to YGL168W 'Protein of unknown function;
reported null mutant phenotype of hydroxyurea sensitivity
may be due to effects on overlapping PMR1 gene'"
/codon_start=1
/product="Hur1p"
/protein_id="EWG86225.1"
/translation="MFILVSVVNICTYIHLHMFPLIRTFTSIGLGVLMKDKGKEGKTI
KAQNVTYQTFEKYVESSSFFFLVHNFLSSSTMKTLLLMSNNNSISEIPSFSVLKILWK
NGIYIAHI"
gene complement(190650..193502)
/gene="Pmr1"
/locus_tag="R008_G10636"
mRNA complement(190650..193502)
/gene="Pmr1"
/locus_tag="R008_G10636"
/product="Pmr1p"
CDS complement(190650..193502)
/gene="Pmr1"
/locus_tag="R008_G10636"
/note="Similar to YGL167C 'High affinity Ca2+/Mn2+ P-type
ATPase required for Ca2+ and Mn2+ transport into Golgi;
involved in Ca2+ dependent protein sorting and processing;
mutations in human homolog ATP2C1 cause acantholytic skin
condition Hailey-Hailey disease'"
/codon_start=1
/product="Pmr1p"
/protein_id="EWG86226.1"
/translation="MSDNPFNASLLDEDSNREREILDATAEALSKPSPSLEYCTLSVD
ETLEKLDTDKNGGLRSSNEANNRRSLYGPNEITVEDDESLFKKFLSNFIEDRMILLLI
GSAVVSLFMGNIDDAVSITLAIFIVVTVGFVQEYRSEKSLEALNKLVPAECHLMRCGQ
ESHVLASTLVPGDLVHFRIGDRIPADLRIIEAIDLSIDESNLTGENEPVHKTSQTIEK
SSFNDQPNSIVPISERSCIAYMGTLVKEGHGKGIVVGTGTNTSFGAVFEMMNNIEKPK
TPLQLTMDKLGKDLSLVSFIVIGMICLVGIIQGRSWLEMFQISVSLAVAAIPEGLPII
VTVTLALGVLRMAKRKAIVRRLPSVETLGSVNVICSDKTGTLTSNHMTVSKLWCLDSM
SNKLNVLSLDKNKKTKNSNGNMKNYLTEDVRETLTIGNLCNNASFSQEHAIFLGNPTD
VALLEQLANFEMPDIRNTVQKVQELPFNSKRKLMATKILNPVDNKCTVYVKGAFERIL
EYSTSYLKSKGKKTEKLTEAQKATINECANSMASEGLRVLGFAKLTLSDSSTPLTEDL
IKDLTFTGLIGMNDPPRPNVKFAIEQLLQGGVHIIMITGDSENTAVNIAKQIGIPVID
PKLSVLSGDKLDEMSDDQLANVIDHVNIFARATPEHKLNIVRALRKRGDVVAMTGDGV
NDAPALKLSDIGVSMGRIGTDVAKEASDMVLTDDDFSTILTAIEEGKGIFNNIQNFLT
FQLSTSVAALSLVALSTAFKLPNPLNAMQILWINILMDGPPAQSLGVEPVDHEVMKKP
PRKRTDKILTHDVMKRLLTTAACIIVGTVYIFVKEMAEDGKVTARDTTMTFTCFVFFD
MFNALACRHNTKSIFEIGFFTNKMFNYAVGLSLLGQMCAIYIPFFQSIFKTEKLGISD
ILLLLLISSSVFIVDELRKLWTRKKNEEDSTYFSNV"
gene 194162..194839
/gene="Cup2"
/locus_tag="R008_G10641"
mRNA 194162..194839
/gene="Cup2"
/locus_tag="R008_G10641"
/product="Cup2p"
CDS 194162..194839
/gene="Cup2"
/locus_tag="R008_G10641"
/note="Similar to YGL166W 'Copper-binding transcription
factor; activates transcription of the metallothionein
genes CUP1-1 and CUP1-2 in response to elevated copper
concentrations'"
/codon_start=1
/product="Cup2p"
/protein_id="EWG86227.1"
/translation="MVVINGVKYACETCIRGHRAAQCTHTDGPLQMIRRKGRPSTTCG
HCKELRRTKNFNPSGGCMCASARRPAVGSEEDETRCRCDEGEPCKCHTKRKSSRKSKG
GSCHRRANDEAAHVNGLGIADLDVLLGLNGRSSDVDMTTTLPSLKPPLQNGEIKADSI
DNLDLASLDPLEQSPSISMEPVSINETGSAYTTTNTALNDIDIPFSINELNELYKQVS
SHNSHSQ"
gene complement(195011..196333)
/gene="Yrb30"
/locus_tag="R008_G10651"
mRNA complement(195011..196333)
/gene="Yrb30"
/locus_tag="R008_G10651"
/product="Yrb30p"
CDS complement(195011..196333)
/gene="Yrb30"
/locus_tag="R008_G10651"
/note="Similar to YGL164C 'RanGTP-binding protein,
inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP
(Gsp1p); shares similarity to proteins in other fungi but
not in higher eukaryotes'"
/codon_start=1
/product="Yrb30p"
/protein_id="EWG86228.1"
/translation="MDEILAKAGSQAVTFAIKSGISIASTYALKTITNFVVQIPKDDA
RRIDQLKFKLESRMAIVSSAIDLIKLVAARGNTNLQITLRLTKDLKEEIDRFDEKINE
MTQKVEGSRSAKTQNEAIKAVENYIKDLLLRIEEITPFINLSLTTSGANLNSALPYQL
SPGLLLKASDFVSENNRKYEKAMKSNEKGTGDKEILKVQVGPTFEVTLFSIFYNLTSE
NNGQSGIVWKEDMKRAKARIYRLNSTGRKYDYFMKIEQDFNDGRYHEDDDKEDTPQEL
AIDLNHIKKLFFSVSGKLLRLEEQDSPVLVLKIDRSDDKENESSEGDKGLLDDITWYA
VSGYEAIEEDEEEDEEEDEEEGKDGEERKEEEEEENKLEDKDSSITLLEYIIRLTSLQ
SNDQKSILEVSDERLSIYLNDENTNSRKDRISNSTIEETEKKLKNLKL"
gene complement(196741..199437)
/gene="Rad54"
/locus_tag="R008_G10661"
mRNA complement(196741..199437)
/gene="Rad54"
/locus_tag="R008_G10661"
/product="Rad54p"
CDS complement(196741..199437)
/gene="Rad54"
/locus_tag="R008_G10661"
/note="Similar to YGL163C 'DNA-dependent ATPase,
stimulates strand exchange by modifying the topology of
double-stranded DNA; involved in the recombinational
repair of double-strand breaks in DNA during vegetative
growth and meiosis; member of the SWI/SNF family'"
/codon_start=1
/product="Rad54p"
/protein_id="EWG86229.1"
/translation="MARRRLPDRPPNGIGAGERPRLVPRPINVQDSVNRLTKPFRVPY
KNTHIPPAAGRIATGSDNIVGGRSLRKRSATVCYSGLDINADEAEYNSQDISFSQLTK
RRKDALSAQRLAKDPTRLSHIQYTLRRSFTVPIKGYVQRHSLPLTLGMKKKITPEPRP
LHDPTDEFAIVLYDPSVDGEMIVHDTSMDNKEEESKKMIKSTQEKDNINKEKNSQEER
PTQRIGRHPALMTNGVRNKSLRELLGDSENSAENKKKFASVPVVIDPKLAKILRPHQV
EGVRFLYRCVTGLVMKDYLEAEAFNTSSEDPLKSDEKALTESQKTEQNNRGAYGCIMA
DEMGLGKTLQCIALMWTLLRQGPQGKRLIDKCIIVCPSSLVNNWANELIKWLGPNTLT
PLAVDGKKSSMGGGNTTVSQAIHAWAQAQGRNIVKPVLIISYETLRRNVDQLKNCNVG
LMLADEGHRLKNGDSLTFTALDSISCPRRVILSGTPIQNDLSEYFALLSFSNPGLLGS
RAEFRKNFENPILRGRDADATDKEITKGEAQLQKLSTIVSKFIIRRTNDILAKYLPCK
YEHVIFVNLKPLQNELYNKLIKSREVKKVVKGVGGSQPLRAIGILKKLCNHPNLLNFE
DEFDDEDDLELPDDYNMPGSKARDVQTKYSAKFSILERFLHKIKTESDDKIVLISNYT
QTLDLIEKMCRYKHYSAVRLDGTMSINKRQKLVDRFNDPEGQEFIFLLSSKAGGCGIN
LIGANRLILMDPDWNPAADQQALARVWRDGQKKDCFIYRFISTGTIEEKIFQRQSMKM
SLSSCVVDAKEDVERLFSSDNLRQLFQKNENTICETHETYHCKRCNAQGKQLKRAPAM
LYGDATTWNHLNHDALEKTNDHLLKNEHHYNDISFAFQYISH"
gene 201143..202042
/gene="Sut1"
/locus_tag="R008_G10671"
mRNA 201143..202042
/gene="Sut1"
/locus_tag="R008_G10671"
/product="Sut1p"
CDS 201143..202042
/gene="Sut1"
/locus_tag="R008_G10671"
/note="Similar to YGL162W 'Transcription factor of the
Zn[II]2Cys6 family involved in sterol uptake; involved in
induction of hypoxic gene expression'"
/codon_start=1
/product="Sut1p"
/protein_id="EWG86230.1"
/translation="MSTSITVRNRDRSLPPLLLPNVSLLEKDIRRKGTQNVGITDPEL
LLTTWMRKRAFPTDELLGGYKRLKPAAADSNECAIGIATVTPPPTLPVSAIVPPPQNY
TPPLFEYHPHALASMVNEDANASCTQMSIISRSTSNSTTSSATSTSSISKRQRSGPSC
DKCRLKKIKCNAKIEILLQDDTIMPMISNKLRYVLTPDDIRLYRGTLLRNIAIPDDVI
EGTGSRKLIKHIDKLVLLTPCLPCIKKKHSSSSTNFPKNDKCTFSKGFTRADINISSK
ISLKFKDKTIYDITYDDYKSIDF"
gene complement(202215..203147)
/gene="Yip5"
/locus_tag="R008_G10676"
mRNA complement(202215..203147)
/gene="Yip5"
/locus_tag="R008_G10676"
/product="Yip5p"
CDS complement(202215..203147)
/gene="Yip5"
/locus_tag="R008_G10676"
/note="Similar to YGL161C 'Protein that interacts with Rab
GTPases, localized to late Golgi vesicles; computational
analysis of large-scale protein-protein interaction data
suggests a possible role in vesicle-mediated transport'"
/codon_start=1
/product="Yip5p"
/protein_id="EWG86231.1"
/translation="MPSNNSSFLDIDDDLEGVDDFGNEPNPFDDATVPDSPNMNNSTA
GKGSEFYNTTGSKAESAPLQGQMDPPAYDQVIGQNDNDGLGRNGLRPGLINYYSKYFQ
IDLTQFKKRLSAVLTFRNDHNSESNEDNTDLYGAVWITATVVMINFTMSRGLNFIISD
VIEGVKTGEDIDRASQFKKLLHSIWLFYGYTFGVPFITMQVLNRDEHSERNRSFKSVP
ELISVYGYANLIWIPVCVILNILDMSKRLRTVQAIQWAIVALGWAQSSYFLNSQISSN
NNTETQSNGKFSLSIIVVVALHTLFCLLFRFIIF"
gene 203566..205278
/gene="Aim14"
/locus_tag="R008_G10681"
mRNA 203566..205278
/gene="Aim14"
/locus_tag="R008_G10681"
/product="Aim14p"
CDS 203566..205278
/gene="Aim14"
/locus_tag="R008_G10681"
/note="Similar to YGL160W 'Protein with similarity to
iron/copper reductases (FRE1-8), possibly involved in iron
homeostasis; may interact with ribosomes; null mutant
displays elevated frequency of mitochondrial genome loss'"
/codon_start=1
/product="Aim14p"
/protein_id="EWG86232.1"
/translation="MKESPLITLVKRHSETHFANIKYGYYVLIISLVYLIGLALLRAF
GRRTPSRSSSAFKNKIIYRLYDIDPAIHLGILFFAVLIPFYYHYSLTTQSTVYLKRLG
RLSYALIPLNLFLTLRPNWFLRKNCTYTDFIPFHKWFSRIITVIGLLHGIFFIIKWAI
DDNVSLKQKLILKTFNFVGFIISILVLFLLICSIGPMRRYNYRLFYIVHNLVNVAFIL
LTPIHSRPGVKFPFLLLNCTLLFIHIINRIVFAKSLMILNKNANYSKTNLVHVRLPRA
ILPDYFEPGSHIRISPYRRINPLYWLLPSHPYTIASLAEDNSIDLIIKETSTAEPGSQ
IESLRSNPKSFHLDQEKTYTLINSYPPSVPEECYSQGTNIAIICGGSGISFALPLFRH
FFNKENVKYLKMIWLIKNYSEYELVLDYLKTNGLTFEKKPSNNKRISVFISGEYTAET
RLDEITTNIDDENSEYEMGSFNNEDEDLSISNFNSENADSNDKTPETSHSPTKENGSL
IEVKSKHSFTLSNELKSFNNESAQVNQNETWLFSCGPPSLLQLSKKYCNDERINFVCE
TYGL"
gene 205726..206839
/locus_tag="R008_G10686"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 208527..208640
/locus_tag="R008_G10691"
tRNA 208527..208640
/locus_tag="R008_G10691"
/product="tRNA-Leu"
/note="TL(CAA)G1"
gene 210039..211577
/gene="Rck1"
/locus_tag="R008_G10696"
mRNA 210039..211577
/gene="Rck1"
/locus_tag="R008_G10696"
/product="Rck1p"
CDS 210039..211577
/gene="Rck1"
/locus_tag="R008_G10696"
/note="Similar to YGL158W 'Protein kinase involved in the
response to oxidative stress; identified as suppressor of
S. pombe cell cycle checkpoint mutations'"
/codon_start=1
/product="Rck1p"
/protein_id="EWG86233.1"
/translation="MSVNPEFIADGIDFYPTTPDAAYFNAADGKNKVNRINGNSENLH
HSFASSCRRSSLSVDFNVTSSDSEKSEQSCLENNSQEDEYFCDIFSTELKLDETSNKS
TDYSSSNHQYPEQLELHNYKLLNKIGEGAFSRVFKAVGINTDDQAPVVIKAIIKKGIS
SDDTLKGNDRIQGSSRKKVLNEVAIHKLVSKNNPHCTKFIAFQESANYYYLVTELVTG
GEIFDRIVQLTCFSEDLARHVITQVAIAIKHRHYMGIVHRDVKPENLLFEPIPFYGLD
GDMQKEDEFTLGVGGGGIGLVKLMDFGLAKKLRNNTAKTPCGTIEYVAPEVFTSKRYS
MKVDMWSIGCVLFTLLCGYPPFYEKNEKTLLKKISRGDYEFLAPWWDNISSGAKNAVT
HLLEVDPNKRYDIDDFLNDPWLNSYDCLKDSNSNSYASVQSILNDSFDERAETLHCAL
SCQSEKQDDTEFSRSESSEYIFMTEEDRNLRGSWIGEPKECFTLDLATSSIYRRRKNM
IFFW"
gene 212052..213095
/locus_tag="R008_G10701"
mRNA 212052..213095
/locus_tag="R008_G10701"
/product="hypothetical protein"
CDS 212052..213095
/locus_tag="R008_G10701"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86234.1"
/translation="MTTDTTVFVSGATGFIALHIVNDLLKAGYTVIGSGRSQEKNDGL
LKKFNNNPKLSMEIVEDIAAPNAFDEVFKKHGKEIKIVLHTASPFHFETTNFEKDLLT
PAVNGTKSILEAIKKYAADTVEKVIVTSSTAALVTPTDMNKGDLVITEESWNKDTWDS
CQANAVAAYCGSKKFAEKTAWEFLKENKSSVKFTLSTINPGFVFGPQMFADSLKHGIN
TSSGIVSELIHSKVGGEFYNYCGPFIDVRDVSKAHLVAIEKPECTGQRLVLSEGLFCC
QEIVDILNEEFPQLKGKIATGEPATGPSFLEKNSCKFDNSKTKKLLGFQFYNLKDCIV
DTAAQMSEVQNEA"
gene 213462..216713
/gene="Ams1"
/locus_tag="R008_G10706"
mRNA 213462..216713
/gene="Ams1"
/locus_tag="R008_G10706"
/product="Ams1p"
CDS 213462..216713
/gene="Ams1"
/locus_tag="R008_G10706"
/note="Similar to YGL156W 'Vacuolar alpha mannosidase,
involved in free oligosaccharide (fOS) degradation;
delivered to the vacuole in a novel pathway separate from
the secretory pathway'"
/codon_start=1
/product="Ams1p"
/protein_id="EWG86235.1"
/translation="MSSEDIIYDPQFKPVQGIYENRLRQFIDTGGDYHDLNLPKFYDK
KRISLDHDHVKVWWYQVSFERGSSPVSPDKRPSWKSIIERDKKGELEFREANINQPFG
PSWSTTWFKVKISLPEDWVKSDEQLLFQWDCSNEGIVIDPKTLIPVTAFSGGERTEYV
LPKTSDGKHFFYIEAGNNGMFGCGAGSTINPPDDNRFFHLRKADIVWPDLDARALYID
FWMLGDAARELPGDSWQKHQARQLGNAVMNLFDPNDRSSVRKCRELLQREYFDSFLES
SKVYEQGESQVLTNVYGIGNCHIDTAWLWPFAETRRKIVRSWSSQCTLMDRFPEYKFV
ASQAQQFKWLLEDHPEFFNKVLIPKIQQSQFFAVGGTWVENDTNIPSGESLARQFFFG
QRFFLKHFGLKSKIFWLPDTFGYSSQMPQLCRLSGIDKFLTQKLSWNNINSFPHSTFN
WAGIDGSQLLTHMPPGNTYTADSHFGDVLRTAKQNKTPECYGSGLMLYGKGDGGGGPT
EEMLQKMRRIRSMNNRNGNVIPKLQVGITVDEFYDDILKRTNQGHDLPTWSGELYFEF
HRGTYTSQAQTKKLMRLSEIKLHDLEWIAAKTSVLYPDSYKYPSKQINELWENVLLCQ
FHDVLPGSCIEMVYKYEAVPMLHNVVKECTSLIDKTVQFLQSQSKADLVEMRTLTWSK
PEKVSEECSLNGSYTSSVTGYDDYIVLANGKLKVIICKKTGVITSVTDETLGVEYLDT
EHGRNKLGANQFVIFDDKPLGWQAWDTELYSVNQYKYVTKPKKVQVSCNTKEKCAVEV
IFQISEKCKIKSVISLNATAVTDAKLSKVDISTTVENWDARNKFLKVEFPVNIRNDFA
SYETQFGITKRPTHYNTSWDVAKFEVCHHKFADYSEYSKGVSILNNCKYGFSTHGNLM
RLSLLRSPKAPDAHADMGTHEIKYAIYPHRGALSSDTVKLAHEFNYCFKYKLPKDIGM
NFDDIISISGDENVILSNIKRGEDDSAVKSNYSLNPRDEQSIVVRVYESLGGESFASL
NTTLNLKRIEKVDNLEMKVYKSLTATRDESNHAINRIPIKLRPFEIASFRLYF"
gene 217126..218256
/gene="Cdc43"
/locus_tag="R008_G10716"
mRNA 217126..218256
/gene="Cdc43"
/locus_tag="R008_G10716"
/product="Cdc43p"
CDS 217126..218256
/gene="Cdc43"
/locus_tag="R008_G10716"
/note="Similar to YGL155W 'Beta subunit of
geranylgeranyltransferase type I, catalyzes
geranylgeranylation to the cysteine residue in proteins
containing a C-terminal CaaX sequence ending in Leu or
Phe; has substrates important for morphogenesis'"
/codon_start=1
/product="Cdc43p"
/protein_id="EWG86236.1"
/translation="MCQATNGPSRVVTKKHRKFFERHLQLLPSSHQGHDVNRMAIIFY
SISGLSIFDVNVSAKYGDHLGWMRKHYIKTVLDDTENTAISGFVGSLVMNIPHATTIN
LPNTLFALLSMIMLRDYEYFETILDKRSLARFVSKCQRPDRGSFVSCLDYKTNCGSSV
DSDDLRFCYIAVAILYICGCRSKEDFDEYIDTEKLLGYIMSQQCYNGAFGAHNEPHSG
YTSCALSTLALLSSLEKLSDKFKEDTITWLLHRQVSSHGCMKFESELNASYDQSDDGG
FQGRENKFADTCYAFWCLNSLHLLTKDWKMLCQTELVTNYLLDRTQKTLTGGFSKNDE
EDADLYHSCLGSAALALIEGKFNGELCIPQEIFNDFSKRCCF"
gene complement(218322..219140)
/gene="Lys5"
/locus_tag="R008_G10721"
mRNA complement(218322..219140)
/gene="Lys5"
/locus_tag="R008_G10721"
/product="Lys5p"
CDS complement(218322..219140)
/gene="Lys5"
/locus_tag="R008_G10721"
/note="Similar to YGL154C 'Phosphopantetheinyl transferase
involved in lysine biosynthesis; converts inactive
apo-form of Lys2p (alpha-aminoadipate reductase) into
catalytically active holo-form by posttranslational
addition of phosphopantetheine'"
/codon_start=1
/product="Lys5p"
/protein_id="EWG86237.1"
/translation="MVKTIEVVSEVSKVAGVRPWAGIFVVEIQEDILADEFTFEALMR
TLPLASQARILNKKSFHDKCSSLCNQLLQLFGCSIVTGLNFQELKFDKGSFGKPLLDN
NRFLPFSMTIGEQYVAMFLVKCVSTDEYQDVGIDIASPCNYGGREELELFKEVFSERE
FNGLLKASDPCTIFTYLWSLKESYTKFTGTGLNTDLSLIDFGAISFFPAEGASMCITL
DEVPLIFHSQWFNNEIVTICMPKSISDKINTNRPKLYNISLSTLIDYFIENDGL"
gene 219317..220342
/gene="Pex14"
/locus_tag="R008_G10726"
mRNA 219317..220342
/gene="Pex14"
/locus_tag="R008_G10726"
/product="Pex14p"
CDS 219317..220342
/gene="Pex14"
/locus_tag="R008_G10726"
/note="Similar to YGL153W 'Peroxisomal membrane peroxin
that is a central component of the peroxisomal protein
import machinery; interacts with both PTS1 (Pex5p) and
PTS2 (Pex7p), peroxisomal matrix protein signal
recognition factors and membrane receptor Pex13p'"
/codon_start=1
/product="Pex14p"
/protein_id="EWG86238.1"
/translation="MSDVVSKDRKALFDSAVSFLKDESIKDAPLLKKIEFLKSKGLTE
KEIEIAMKEPKKDGIVGDEVSKKIGSTENRASQDMYLYEAMPPTLPHRDWKDYFVMAT
ATAGLLYGAYEVTRRYVIPNILPEAKSKLEGDKKEIDDQFSKIDTVLNAIEAEQAEFR
KKESETLKELSDTIAELKQALVQTTRSREKIEDEFRIVKLEVVNMQNTIDKFVSDNDG
MQELNNIQKEMESLKSLMNNRMESGNAQDNRLFSISPNGIPGIDTIPSASEILAKMGM
QEESDKEKENGSDANKDDNAVPAWKKAREQTIDSNASIPEWQKNTAANEISVPDWQNG
QVEDSIP"
gene 220565..223963
/gene="Nut1"
/locus_tag="R008_G10736"
mRNA 220565..223963
/gene="Nut1"
/locus_tag="R008_G10736"
/product="Nut1p"
CDS 220565..223963
/gene="Nut1"
/locus_tag="R008_G10736"
/note="Similar to YGL151W Component of the RNA polymerase
II mediator complex, which is required for transcriptional
activation and also has a role in basal transcription"
/codon_start=1
/product="Nut1p"
/protein_id="EWG86239.1"
/translation="MEKESVYNLALKCAERQLTSMEFSNLYKEFFNEKFPSLIQEEEE
DTTTTANINEVKKASDLVDTPSNNTAATADTTHLHEALDIFCSDFVKILNLEKPLILA
DYIVEVLLVNYNSDMIKCFLPKLNSVRNSLLLAHFFSKSCSFFAKLSDTLIIDQVRKD
LGNVIVPNILSLDMNSMNKELIAIVSKLLQTTLKLSPSPILLTSASCKNGSFTLLNQL
SQTNKLLFKRVSQTFEEKLHFKDTKPFLNKDSTNEFVGSPSLTSPQYIPSPLSSTKPP
GSVNSAAKYKDMKLLRYYKNIWLNNKIINWEISNPDFLSKYSAITSSIFQESFNSVQN
LDQLLTDLIETSFTCFAQFVSNKQYHQANSNLTLLERKWVIFITKHLPLLILENSSRS
PRVVTNALDNIDEKVVKAIRIYFTEKDDNKTNNEDLFDDYPSTSLDIRHDFIKGLIML
NLQPASVINNYLREDQMIDTSILPTSDDLVVRNLQGIQEVVHNTNSFIISSLDTLELE
SITESITHDSSNGLFQVLHNFESVAPTKQREIVKAFLSIFEDAIKELNYNRIAKICAL
LFFNFSHSLTTILSFSSPAALMKTLIKFVDLSRNGRNGSNGNDESSEYETINISLSFS
WAILLIINLTQTYGISVVDVALKYPELSIKNSFIINFISNLPNVSDKYYLEESNVNDS
DMLTKSHNTVQSWLCDLFVNGSITDQLIQNIETRQLANLIPFIVKQVLLSVEIGVLTD
ISSLIGGFEYFLQPLLLVGLIKTFYWLEQFLSCVKNDTISEDILQGIFNLLNTLFNPV
TLNEDSKAFHTAVLRLNAIPLLKVLRKFRVQSQSNYGIYSSDAQGDPNLEPLIAKLVA
VLNVSPVYDVDPRIINSENDYSRKQLGYGKFLILNENPINKIMTNQINSFWSLHSSTY
YNLDYLFELIELVTPKSFLFDVLKTLEYKLATYGVPGSENKRGSLDSEHVFDYFFYFL
VLYDVKTAEEASQLIEYMENDAKKSKGDVDIKGEDLHEKNDSAEVRQETQPKAEATQD
DDFDMLFGENDTSTQAYEEEEENEDNDGNNRTNNVPMIKAEETPSKTNKISILKRHSF
AVLLHERKLLNDLALENGEITKTENEKFISYHDKYLCMLKTCVF"
gene complement(224145..228614)
/gene="Ino80"
/locus_tag="R008_G10741"
mRNA complement(224145..228614)
/gene="Ino80"
/locus_tag="R008_G10741"
/product="Ino80p"
CDS complement(224145..228614)
/gene="Ino80"
/locus_tag="R008_G10741"
/note="Similar to YGL150C 'ATPase and nucleosome spacing
factor, subunit of complex containing actin and
actin-related proteins that has chromatin remodeling
activity and 3' to 5' DNA helicase activity in vitro; has
a role in modulating stress gene transcription'"
/codon_start=1
/product="Ino80p"
/protein_id="EWG86240.1"
/translation="MSLAVLLNKEDKDISDFSKTTAGKSAKKNSRERVADVAPTRVLD
KKQAYLSQLNSEFNRIKRRDSIEQLYQDWKFINLQEFELISEWNQQSKDWQFDNTNDS
QDLHFKKLYRDMSMINKEWAEYQSFKNANLSDIINEKDADEDEEDDEDELEDGEEDME
EDEASTGRHTNGKSMRGNGIQKSRKKDAAAAAAIGKAIKDDQTHADTVVTVNGDENED
GNNGEDEDNDNDNENNNDNDNDNENENDNDSDNDDEEENGEEDEEEEEIEDLDEEDFA
AFEEQDDNDDEDFNPDVEKRRKRSSSSSSSTKLSMNSLSLITSKKINKNITINSDRPK
IVRELIKMCNKNKHQKIKKRRFTNCIVTDYNPIDSKLNIKITLKQYHVKRLKKLINDA
KREREREEALKNNVGLDGNDLDNDEDGSESHKRRKLNNNTANGADDANKRKFNTRHGL
PTYGMKMNAKEARAIQRHYDNTYTTIWKDMARKDSTKMSRLVQQIQSIRSTNFRKTSS
LCAREAKKWQSKNFKQIKDFQTRARRGIREMSNFWKKNEREERDLKKKIEKEAMEQAK
KEEEEKESKRQAKKLNFLLTQTELYSHFIGRKIKTNELEGNNVSSNDSESQKNIDISA
LAPNKNDFHAIDFDNENDEQLRLRAAENASNALAETRAKAKQFDDHANAHEEEEEEDE
LNFQNPTSLGEITIEQPKILACTLKEYQLKGLNWLANLYDQGINGILADEMGLGKTVQ
SISVLAHLAENHNIWGPFLVVTPASTLHNWVNEISKFLPQFKILPYWGNANDRKVLRK
FWDRKNLRYNKNAPFHVMVTSYQMVVTDANYLQKMKWQYMILDEAQAIKSSQSSRWKN
LLSFHCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFNEWFSKDIESHAEANT
KLNQQQLRRLHMILKPFMLRRVKKNVQSELGDKIEIDVLCDLTQRQAKLYQVLKSQIS
TNYDAIENAATNDSTSNSASNSGSDQNLINAVMQFRKVCNHPDLFERADVDSPFSFTT
FGKTTSMLTASVANNNSSVISNSNMNLSSMSSNNISNGKFTDLIYSSRNPIKYSLPRL
IYEDLILPNYNNDVDIANKLKNVKFNIFNPSTNYELCLFLSKLTGEPSLNEFFRVSTT
PLLKRVIERTNGPKNTDSLSFKTITQELLEVTRNAPSEGVMASLLNVEKHAYEREYLN
CIQRGYHPNVSAPPVTIEVLGSSHVTNSINNELFDPLISQALSDIPAITQYNMHVKKG
IPVEDFPKTGLFPEPLNKNFSSNISMPSMDRFITESAKLRKLDELLVKLKSEGHRVLI
YFQMTKMMDLMEEYLTYRQYNHIRLDGSSKLEDRRDLVHDWQTNPEIFVFLLSTRAGG
LGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRA
KQKEQVQQVVMEGKTQEKNIKTIEVGENDSEVTREGSKSISQDGIKEAASALA"
gene 229440..230570
/gene="Aro2"
/locus_tag="R008_G10751"
mRNA 229440..230570
/gene="Aro2"
/locus_tag="R008_G10751"
/product="Aro2p"
CDS 229440..230570
/gene="Aro2"
/locus_tag="R008_G10751"
/note="Similar to YGL148W Bifunctional chorismate synthase
and flavin reductase, catalyzes the conversion of
5-enolpyruvylshikimate 3-phosphate (EPSP) to form
chorismate, which is a precursor to aromatic amino acids"
/codon_start=1
/product="Aro2p"
/protein_id="EWG86241.1"
/translation="MSTFGKLFRVTTYGESHCKSVGCIVDGVPPGMSLTEADIQPQLT
RRRPGQSKLSTPRDEKDRVEIQSGTEFGKTLGTPIAMMIKNEDQRPHDYSDMDKFPRP
SHADFTYSEKYGIKASSGGGRASARETIGRVASGAIAEKFLAQNSNVEIVAFVTQIGE
IKMNRDSFDPEFQHLLNTITREKVDSMGPIRCPDASVAGLMVKEIEKYRGNKDSIGGV
VTCVVRNLPTGLGEPCFDKLEAMLAHAMLSIPASKGFEIGSGFQGVSVPGSKHNDPFY
FEKETNRLRTKTNNSGGVQGGISNGENIYFSVPFKSVATISQEQKTATYDGEEGILAA
KGRHDPAVTPRAIPIVEAMTALVLADALLIQKARDFSRSVVH"
gene complement(230795..231370)
/gene="Rpl9a"
/locus_tag="R008_G10756"
mRNA complement(230795..231370)
/gene="Rpl9a"
/locus_tag="R008_G10756"
/product="Rpl9ap"
CDS complement(230795..231370)
/gene="Rpl9a"
/locus_tag="R008_G10756"
/note="Similar to YGL147C Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl9Bp and
has similarity to E. coli L6 and rat L9 ribosomal
proteins"
/codon_start=1
/product="Rpl9ap"
/protein_id="EWG86242.1"
/translation="MKYIQTEQQIEVPEGVTVSIKSRIVKVVGPRGTLTKNLKHIDVT
FTKVNNQLIKVAVHNGDRKHVAALRTVKSLVDNMITGVTKGYKYKMRYVYAHFPINVN
IVEKDGAKFIEVRNFLGDKKIRNVPVRDGVTIEFSTNVKDEIVLSGNSVEDVSQNAAD
LQQICRVRNKDIRKFLDGIYVSHKGFITEDL"
gene complement(231792..232727)
/locus_tag="R008_G10761"
mRNA complement(231792..232727)
/locus_tag="R008_G10761"
/product="hypothetical protein"
CDS complement(231792..232727)
/locus_tag="R008_G10761"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86243.1"
/translation="MEKASLNIAQTKEIPLTDRRMGLRGWKACSRPHLLGRILLFMSI
LFITSAELSSDVSSREVYMPIFNNKLSFKVPPIKRSLLLGAALYEDFEYSSNNSASDG
AFCTVFNAGMNDASREVVFEIHVMDVLQEETDSSRFGGTSHERGRQSLGFSVFNNKNG
DLLRSKKNLASGTSVIEVNPGNCNEFLICFINLVYDGSWSSIDTEKSVTIKMTYNDKL
DPDMLLHLVNQMTPQVVKALNTVSDGLFQIVSDTTLLQMESDRRDINEATYSYLIVGF
VSLMVAQLISNIIVTTYLIIKIKSNPSSHIKKKGL"
gene 233284..235389
/gene="Tip20"
/locus_tag="R008_G10766"
mRNA 233284..235389
/gene="Tip20"
/locus_tag="R008_G10766"
/product="Tip20p"
CDS 233284..235389
/gene="Tip20"
/locus_tag="R008_G10766"
/note="Similar to YGL145W 'Peripheral membrane protein
required for fusion of COPI vesicles with the ER;
prohibits back-fusion of COPII vesicles with the ER; forms
a tethering complex with Sec39p and Dsl1p that interacts
with ER SNAREs Sec20p and Use1p'"
/codon_start=1
/product="Tip20p"
/protein_id="EWG86244.1"
/translation="MNGIDDLLNINDRIKQVQNERNELASKLQNLKQSLASNDTEVAL
SEVIAQDIIEVGASVEGLEQLRAKYGDLQILNKLEKVAVQQTQMQAGVDKLDSFERQL
DELAEQPPDQFTLDDLKALHSKLTSVFATVPQINNIDSQYAAYNKLKSKVTGKYNDVI
IQRLATNWSNTFDQKLLEAQWDTQKFASTSVGLVKCLRENSTKLYQLSLLYLPLEEET
QNGDSERPLSRSNNNQEPVLWNFKSLANNFNVRFTYHFHATSSSSKIETYFQFLNDYL
TENLYKCINIFHDDCNGLTKPVIHEQFINYVLQPIRDKVRSTLFQNDLKTLIVLISQI
LATDKNLLNSFHYHGLGLVSLISDEVWEKWINYEVEMANRQFINITKNPEDFPKSSQN
FVKLINKIYDYLEPFYDLDFDLLVRYKLMTCSLIFMNLTSSYLDYILTVDSLNETRTK
EQELYQTMAKLQHVNFVYRKIKSLSSNFIFIQLTDIVNSTESKKYNSLFQNVENDYEK
AMSTDMQNSIVHRIQKLLKETLRNYFKISTWSTLEMSVDENIGPSSVPSAELVNSINV
LRRLINKLDSMDIPLAISLKVKNELLNVIVNYFTESILKLNKFNQNGLNQFLHDFKSL
SSILSSPSHATNYKCMSLHELVKILKLKYDPNNQQFLNPEYIKTGNFTSLKEAYSIKY
LKDTKIQDALYRIIYGNIL"
gene complement(235491..237548)
/gene="Rog1"
/locus_tag="R008_G10771"
mRNA complement(235491..237548)
/gene="Rog1"
/locus_tag="R008_G10771"
/product="Rog1p"
CDS complement(235491..237548)
/gene="Rog1"
/locus_tag="R008_G10771"
/note="Similar to YGL144C Protein with putative serine
active lipase domain"
/codon_start=1
/product="Rog1p"
/protein_id="EWG86245.1"
/translation="MSLTPTNEILFHYKSSVKVGELERYVITYHLYDGEEIPPDLNLN
SLWLKVRNMNPLSYRAAYLMGPFMLYCDVKTAQYHHSQKIVASVDYPKFEPNVQTQQD
FVAELSVHNIRQKYVWIADVMSQILFTTNTNVTYEVTIGTSKESVENPHDLPSHLGSY
SPKLTVNRLTTLDLWNLPVQITTPQKKKHLVVLTHGLHSNVSTDLVYIMEQIYKAQKN
YPHEQIVVKGYRGNVCQTEKGVKYLGTRLAEYIIQDLYDESIRKISFVGHSLGGLIQA
FAIAYIYEVYPWFFKKVNPINFITLASPLLGIVTDNPAYIKVLLSFGVIGKTGQDLGL
ENDVEVGKPLLYLLSGLPLIEILRRFKRRTVYANAINDGIVPLYTASLLFLDYNDILE
QLQKLKENSKKSPLINDASTPVNQDFFNKTFISPLTKMLSILAPQKFPTENGSEIPKV
SFFESASSILLPPLPERAYIMDPDSRDPVIIHDKIYNEDDIPQSEFDIEDGFFDKKNI
LLQAFFAGKKERAKYRNLEETIARRWHEGMAWRKVVVALKPDAHNNIIVRRKFANAYG
WPVIDHLIDVHFNGDDDDDNDENDDINSTQVVEPIQSVTEGKKKYRKAENIPQEYGWL
NKVETNGVFDEGPTGMISTVGEIVEALAKRGFSAVIDRRNASEDPNDEVLRFEEMNSD
LVQ"
gene complement(237758..238999)
/gene="Mrf1"
/locus_tag="R008_G10776"
mRNA complement(237758..238999)
/gene="Mrf1"
/locus_tag="R008_G10776"
/product="Mrf1p"
CDS complement(237758..238999)
/gene="Mrf1"
/locus_tag="R008_G10776"
/note="Similar to YGL143C 'Mitochondrial translation
release factor, involved in stop codon recognition and
hydrolysis of the peptidyl-tRNA bond during mitochondrial
translation; lack of MRF1 causes mitochondrial genome
instability'"
/codon_start=1
/product="Mrf1p"
/protein_id="EWG86246.1"
/translation="MWLSKFQFPSRSIFKGVFLGHKLPLLVRLTSTTTNSKSNGSIPT
QYTELSPLLVKQAEKYEAELKDLDKDLSCGIHFDVNKQKHYAKLSALTDTFIEYKEKL
NELKSLQEMIVSDPSLRAEAEQEYAELVPQYETTSSRLVNKLLPPHPFADKPSLLELR
PGVGGIEAMIFTQNLLDMYIGYANYRKWKYRIISKNENESGSGIIDAILSIEEAGSYD
RLRFEAGVHRVQRIPSTETKGRTHTSTAAVVVLPQIGDESAKSIDAYERTFKPGEIRV
DIMRASGKGGQHVNTTDSAVRLTHIPSGIVVSMQDERSQHKNKAKAFTILRARLAEKE
RLEKEEKERKARKSQVSSTNRSDKIRTYNFPQNRITDHRCGFTLLDLPGVLSGERLDE
VIEAMSKYDSTERAKELLESN"
gene complement(239310..241160)
/gene="Gpi10"
/locus_tag="R008_G10781"
mRNA complement(239310..241160)
/gene="Gpi10"
/locus_tag="R008_G10781"
/product="Gpi10p"
CDS complement(239310..241160)
/gene="Gpi10"
/locus_tag="R008_G10781"
/note="Similar to YGL142C 'Integral membrane protein
involved in glycosylphosphatidylinositol (GPI) anchor
synthesis; putative alpha 1,2 mannosyltransferase required
for addition of the third mannose onto the GPI core
structure; human PIG-Bp is a functional homolog'"
/codon_start=1
/product="Gpi10p"
/protein_id="EWG86247.1"
/translation="MAHEVHRIKPKLGRTQIFWVFLAFRVLNAVLTRTFFQADEFWQA
LEPAHWKAFKYGELTWEWKFGVRSYLFPMIFELTYRLVSLSSILLHYALLLLSTIGSD
LLILLLPKYELSWQVAEDLKRLPFDVTRSFEYYGVIYAPKIVMAVLASIGEYYIVRFV
QKLYLLTLDKRNEKEEEERRSGLSEITKFALLLSLTNFFNCFFITRTFINSFEMILTS
IALYYWDWTGGQMIKESSFTKSLIFAFLACLQRPSSGLIWVIPSISLILNLVGKKQYH
LLFITFSKVLRSFFLVFTANAIIDMYFYEKVTFPFFRFLKFNFTTPLSKFYGVAPWHF
HFFQSLPIVLGASIPAFAFGLFFPLSKRSFPKKYLNPFFQVKLTILLNLLVYSTLPHK
EFRFIFPLQPLFILISSFGLLRLDRDYWKRLSGLKSLLWLVPFVSVFIALLLDTFHES
GSIEVMKFLHEEPEIDSLGFIMPCHSTPGQSYLHRSDIQDLWSITCNPPLHLLGDPEA
YSKLETYMDESDHLYDDISAFIYKNFPPPFRKDLRSPGKTYSHEWPTYLVVFEHMENA
FLKDFLKDSSYIEYNRFFNSLAHWDSRRSGDIIIYYKLPFDYSDIPAADI"
gene 241394..244126
/gene="Hul5"
/locus_tag="R008_G10786"
mRNA 241394..244126
/gene="Hul5"
/locus_tag="R008_G10786"
/product="Hul5p"
CDS 241394..244126
/gene="Hul5"
/locus_tag="R008_G10786"
/note="Similar to YGL141W 'Multiubiquitin chain assembly
factor (E4); proteasome processivity factor that elongates
polyUb chains on substrates, opposing Ubp6p, a branched
polyubiquitin protease; required for retrograde transport
of misfolded proteins during ERAD'"
/codon_start=1
/product="Hul5p"
/protein_id="EWG86248.1"
/translation="MLNFTGQTRRRNVNLGNRTRNSKKDLLEKAKRERERRAQDKLKE
DASKTIQKSIRRHFSNVRLFKNTFTSSQLVHMIPAYGGKLIYYISQYDLQQLLKLSHN
FLSSYPNSLGNRQLLSLLKLYQDDALVAETLSDLNMDCPTVDEFLDSLSVYLCRASFL
SYSSASKLADVIEAWEVMHSSASISIFSISIGSYEKRPFALQFYCILAERNLLPQLIN
TNPILWDNMAKTYSHCSKGGQKNIAKLLIPNFNNHIAPSVLRSDNDYVLKFYEKAFID
EVIATTANYVSDEDHVKNLMCYIASSPNQSCKNSVLITLLSNKDFVRRLSWEFFHTKF
NASKTEAHPLFSVLAQLIDMHLLISTDRELLDYNSVIPIEELKKFTSTLKDFTFRQYW
ELPKSERNPMLKEAVPLLSKVYERDSRLHFLSTENNPTYWENSEKQFLNLRFYEELQE
YEDLYREHLEEESDEDMEKEIDLDKERPPLKSLLLNKMKKRLKSSLRFRKLEILLELP
FFIPFEERVDLFYMFIALDKKRLSLDDDHNLINMFTPWASTGMRKQSAIISRDNVLED
AFNAFNSIGERFKASLDVTFINEFGEEAGIDGGGITKEFLTTVSDEGFKDPKHELFRT
NDRYELYPSVVYDATKLKYIWFLGKVVGKCLYEHVLIDVSFADFFLKKLLNYSNGFLS
SFSDLGSYDSVLYNNLIKLLNMTTDEIKSLDLTFEIDEPESSAKVVDLIPNGSKTYVT
KDNVLLYVTKVTDYKLNKRCFKPVSAFHGGLSVIIAPHWMEMFNSIELQMLISGERDN
IDLDDLKSNTEYGGYKEEDQTIVDFWEVLNEFKFEEKLNFLKFVTSVPQAPLQGFKAL
DPKFGIRNAGTEKYRLPTASTCVNLLKLPDYRNKTILREKLLYAINSGARFDLS"
gene complement(244394..248053)
/locus_tag="R008_G10791"
mRNA complement(244394..248053)
/locus_tag="R008_G10791"
/product="hypothetical protein"
CDS complement(244394..248053)
/locus_tag="R008_G10791"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86249.1"
/translation="MSLKSKLTKIQKLWLYYFPCDRILAKRICKSTVNTTVAFIFCLI
PKITAHLGAAPAMLPMISVIVHPGRRVGGTIHGAIYCITGLIFGLAYAIFGRFLAQRC
LGSSWHELTEAQQHVLHYKRYEAGLAILAVFEVIMLFFHGWMRSVSHYYFGIVFPLFV
VVHFAFMDPLNETAGTIAKAYSTPFYLGIAMSIFWNLVLFPEWGTTYLGNTTIDAMNE
LHKSIDYSINFFIAVDPHNSSQLYSRDPVSLGKLLKMKSLISSKVNNCRVVLHECIYE
FTYAYVSPTKLKPIISTLENLTVFINGLVNTCQLEFILLARHDNKLRPDDVAALTLPK
NKEISFANAEKLLKVIDKLHPVIYSLHRTMSECMYIAKLVLAHAFDVKVSRVHSCSMF
KDGNFPTFSNNANNLPNDVDIQNKINDLKQALEECKAKFKSEMLSFDIDIMSPSDEMF
LLSSFLLNFRQTADSTLVIMESVKDILVKRQIQEKKGWLRGKRLWFLVLTNYETFSIW
LKGDRNSVTENDTLKGTFNGNTNGFAHDTVIRRPDYEENELLSQKVSSNKNIVKDDAS
LDLPMTSEPKGNSSSTSDTSSSPLTLTKTTTFGTNRTSRRQGRFSFMSMLISIDKFCE
VSHPHFRFGFQVAIALMLASFPMFIPKTRQWYIDYRGTWIGFVCILCLEPSVGGTFWV
FFLRAVGVIFGAAWGYLSYVAAVNQTNPYLETVITVFGAIPGFYYLLGTPYVKAAIIE
IISIYIVMLAAILPSQDDILTSFAKRCLAVGYGGGVALIVQVFFFPLKAREQLNEEIS
FVCGCISEMELLYATGLEGEQVASSMSEEKYKKIEKISKSAKEALARATAYKGLTRQE
PRLKGEYTELENVFTQVIFIQKQIIERIDTISLLRKQNGSAVIEEFNSVVYPYRRQMV
GSISCLMRALQEAFINKTPLPQFLPSARIAHRRLINKVRQTLRIRYPGQISNLSDKAR
KPNEGDYADGKDEDDDDNEGLVMKMNRRGQANTTANPHEYVLKEKFLSWNASSAASEE
IIEYIEELLNLTKILVGVNEFKYGFLSRPLYEDWAAEAVTGFDNFINGKSNPMNTRRN
RTPFDGTSIISEGNESLQSTNSNESQISPDSTRSYEPECPVAYEKNDNPAALNLLRIA
SHKAGQNADGLPKTFRNRAFSIASTSGQLSSLSRHSTLGNADPNYLNDDDESSDDDLP
LALKMVLSHMKEKKD"
gene 248757..251165
/gene="Flc3"
/locus_tag="R008_G10796"
mRNA 248757..251165
/gene="Flc3"
/locus_tag="R008_G10796"
/product="Flc3p"
CDS 248757..251165
/gene="Flc3"
/locus_tag="R008_G10796"
/note="Similar to YGL139W 'Putative FAD transporter,
similar to Flc1p and Flc2p; localized to the ER'"
/codon_start=1
/product="Flc3p"
/protein_id="EWG86250.1"
/translation="MRFLQVYKSSALIGLIILLASKVNLAEAKRKLVATSLVTCMENS
QLSANSFDVVFNPDDRSLHYDLDMSTQIDSYIFADIDVYAYGFKIITKNVDLCSINWK
QFCPVHPGNIQIDSIEYISSEYVNEIPGIAYQVPDIDAYARVKITNNVSEYLACIQIY
FSNGKTVSQIGVKWATAVVAGIGLLLSAILSTFGNSTAASHISANTMSLFLYFQSVVV
VAMQHVHRVPPIAAAWAENLVWSMGLIRISFMQRIFRWYVQSTGGTPSLYLTSTSMSV
LAQRSWQYLMELPLIKRATNVLYGNANTLIFRGIKRLGYKMGIENTSIVCTGFTFFVL
CGYVLAGFIIVFKCCVELATRLGWIQKARFWEFRKQWRMILKGALLRYIYIGFVQLTI
LSFWEFTERDSPAVIVIACLFILLSCGLMLWAAWRTVFFARRSVALYNNPAALLYGDE
YVLHKYGFFYTMFNANHYWWNIVLLSYIFVKSLLVGFAQASGQTQVLFMFILDLFYFV
AIIYYKPYLDRPTNIMNILIATVTVVNSFLFMFFSDLFNQSYKVAAIMGWIFFIMNAA
FSFILLMMILAFAGMMLFSKNPDLRFKPAKDDRTSFQRNTMKPEGTVNRSVANELLAL
GNVAKDHDDNSDYESNDTGVNDELKQAQDETTPTTVTSSDDNKPTFSEKILSKFSRPK
NENASTDALRVEAPKQQTFPHNLTNLSRENLSTLGSKPYPGHTRSQSDAHNGLINSFE
EEDTSSNTDPFHDSTEGDLLDTSSSDGGFRSQNYVRDDSINSLGNNKQPLRKPPGFFD
EGFM"
gene complement(251534..252571)
/locus_tag="R008_G10801"
mRNA complement(251534..252571)
/locus_tag="R008_G10801"
/product="hypothetical protein"
CDS complement(251534..252571)
/locus_tag="R008_G10801"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86251.1"
/translation="MYNFLEFFFFFITYTLFKSTFVQGKSSFPGHDVCKFEDQNFQTE
FFLNVLKGDKLQNLKQEYEQYKKQSTLYTGFVIEKQYEYQVAPLQINNFLQVTFCKGG
KPIWNHILPFQKDLDWAEPLCIPAQEDDTISQNSSVCFKFARVQKYTQRNVTLYFPNK
FVGFVFVCNSSKTHPPTQNNFETIPLTPIISDDIRDYKISWSIKGRITKVVSYLHTLS
IPMSKYEMLIHSDKNISNELEYKSLTSEFWKSYKNLRKFKNKEMSWINEINPMEQYDS
SGNENVPNFHEKLDRTINRIAQNIERPHDALIRAIAAHNRNSNSGNITRRKYLRRKIS
KMLKNKIPLKN"
gene 252909..255778
/gene="Sec27"
/locus_tag="R008_G10806"
mRNA join(252909..252926,253127..255778)
/gene="Sec27"
/locus_tag="R008_G10806"
/product="Sec27p"
CDS join(252909..252926,253127..255778)
/gene="Sec27"
/locus_tag="R008_G10806"
/note="Similar to YGL137W 'Essential beta'-coat protein of
the COPI coatomer, involved in ER-to-Golgi and Golgi-to-ER
transport; contains WD40 domains that mediate cargo
selective interactions'"
/codon_start=1
/product="Sec27p"
/protein_id="EWG86252.1"
/translation="MKLDIKKTFSNRSDRVKGIDFHPTEPWVLTTLYSGRVEIWNYET
QVEVRSIQVTETPVRAGKFIARKNWIIVGSDDFRIRVFNYNTGEKVVDFEAHPDYIRS
IAVHPTKPYVLSGSDDLTVKLWNWENNWALEQTFEGHEHFVMCVAFNPKDPSTFASGC
LDRTVKVWSLGQSTPNFTLTTGQERGVNYVDYYPLPDKPYMITASDDLTIKIWDYQTK
SCVATLEGHMSNVSFAVFHPTLPIIISGSEDGTLKIWNSSTYKVEKTLNVGLERSWCI
ATHPTGRKNYIASGFDNGFTVLSLGNDEPTLSLDPVGKLVWSGGKNAAASDIFTAVIR
GNEEVEQDEPLSLQTKELGSVDVFPQSLAHSPNGRFVTVVGDGEYVIYTALAWRNKAF
GKCQDFVWGPDSNSYALIDETGQIKYYKNFKEVTSWSVPMHSAIDRLFSGALLGVKSD
GFVYFFDWDNGTLVRRIDVNAKDVIWSDNGELVMIVNTNSNGDEASGYTLLFNKDAYL
EAANNGNIDDSEGVDEAFDVLYELSESITSGKWVGDVFIFTTATNRLNYFVGGKTYNL
AHYTKEMYLLGYLARDNKVYLADREVHVYGYEISLEVLEFQTLTLRGEIEEAIENVLP
NVEGKDSLTKIARFLEGQEYYEEALNISPDQDQKFELALKVGQLTLARDLLTDESAEM
KWRALGDASLQRFNFKLAVEAFTNAHDLESLFLLHSSFNNKEGLVTLAKDAERAGKFN
LAFNAYWIAGDIQGAKDLLIKSQRFSEAAFLGSTYGLGDDAVNDIVTKWKENLILNGK
NTVSERVCGAEGLPGSSSSGDAQPLIDLDSTPAPEQADENKEAEVEDSEFKESNSEAV
EAEKKEEEAPQQQQSEQQPEQGEAVPEPVEEES"
gene complement(255937..256899)
/gene="Mrm2"
/locus_tag="R008_G10811"
mRNA complement(255937..256899)
/gene="Mrm2"
/locus_tag="R008_G10811"
/product="Mrm2p"
CDS complement(255937..256899)
/gene="Mrm2"
/locus_tag="R008_G10811"
/note="Similar to YGL136C 'Mitochondrial 2' O-ribose
methyltransferase, required for methylation of U(2791) in
21S rRNA; MRM2 deletion confers thermosensitive
respiration and loss of mitochondrial DNA; has similarity
to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ'"
/codon_start=1
/product="Mrm2p"
/protein_id="EWG86253.1"
/translation="MILVYNRIRSIISSSLGRIHVRYNSNSQNRWLNRQLKDPYTKEA
KVQNLRSRAAFKLMQIDDKYRLFSKNRTDQRILDLGYAPGAWSQVARQRSSPNSMILG
VDILPCEPPHGVNSIQANILAKRTHDLIRLFFSKHFQLNRHDDLHKDHGYFQNMLEEE
LTHVKDTELYREIFTSDDIYETPNTNSTLIEREKFPVDVIISDMYEPWPQTTGFWNNI
TNQAYFRMANTSGVSIRDHYQSIDLCDAALVTAIDLLRPLGSFVCKLYTGEEENLFKK
RMQAVFTNVHKFKPDASRDESKETYYIGLKKKRNVDKLDVFSNS"
assembly_gap 257705..258184
/estimated_length=480
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 258724..260025
/gene="Pcl10"
/locus_tag="R008_G10816"
mRNA 258724..260025
/gene="Pcl10"
/locus_tag="R008_G10816"
/product="Pcl10p"
CDS 258724..260025
/gene="Pcl10"
/locus_tag="R008_G10816"
/note="Similar to YGL134W 'Pho85p cyclin; recruits,
activates, and targets Pho85p cyclin-dependent protein
kinase to its substrate'"
/codon_start=1
/product="Pcl10p"
/protein_id="EWG86254.1"
/translation="MDMTKNHTTDTEEFDDGDIRPVSLGIVDDYNTSFELPLKPRFLQ
SENFSDLTSEWDQSRSNTPGLAEGKTEKAQPCGTTDSSKNRIHVEQLLESANEMNNYL
AQNIENINNFQVGLLNGGKGLYSSMGDDSSACINGTNFSSTSNFELSDDELEDTTGCT
SSIFDKDLFHQQNGLSIPRRRSPLFKSPTASFEIGDATDVEEQDIDDSIFSECSSITS
FDMGGLHISLPHDEEEDQEKTKSESENPLLHGIPVDVEVPHISVDEALANFKETIELL
LKLSGNRKCTGFNTRVEKKEYSNFYMKSKPTLSSADFLKRIQDKCEYQPTVYLVATFL
IDTLFLTRDGNNILQLKLNLQEKEVHRMIIAAVRLSTKLLEDFVHSHEYFSKVCGTSK
RLLTKLEVSLLICVCNTKLMVSNRKLAASKLLLNELRSFCV"
gene 260768..264562
/gene="Itc1"
/locus_tag="R008_G10821"
mRNA 260768..264562
/gene="Itc1"
/locus_tag="R008_G10821"
/product="Itc1p"
CDS 260768..264562
/gene="Itc1"
/locus_tag="R008_G10821"
/note="Similar to YGL133W 'Subunit of the ATP-dependent
Isw2p-Itc1p chromatin remodeling complex, required for
repression of a-specific genes, repression of early
meiotic genes during mitotic growth, and repression of
INO1; similar to mammalian Acf1p, the regulatory subunit
of the mammalian ATP-utilizing chromatin assembly and
modifying factor (ACF) complex'"
/codon_start=1
/product="Itc1p"
/protein_id="EWG86255.1"
/translation="MVLYKRKPILLPDPKPLPLDLNVQVWHIEETGEWFSSYEEFLER
FDFYTRHHFTCEITGTSCLTFFQALDSEETQFKYVEDRFPLKLREPVARFLHFNGIRR
LDALVEKVYARFKNDFFPGEVVYLRKQKDSSTTSSNSQQSTPQPDDMVEINSVGNPGL
PQYQYQRRYVIKEKVQFNATINPESREIVMPAHTKYMLIEEAASSNKSFIVDQGQIYR
DRSTFTKHLIKCFFKITLQRASSKMGAPWCVKPEYLAMYGLTMEWPKDMLKYKEDEPV
VARRSNSANVSSPESEKNKRQSKSSGKSNTSNDASNKKETKKKRKPTEVNDSENNSSE
EDKKKGQNVTSETHSKKRKKEANEEPNTENVESVPTPANAEPQAVTITSIMDDLALPY
QHPPNIFPNLTYYNEKLECISLGSTKLSRPFDSFGKLLQAYQFLNTFGSKICLSHFSL
DQFITSLKCTDPYELKGEVVLVNIRTQTSKEQEIENNGLPIKNKAETTTEEDSENPSD
WQRNSFIRDMIMKRNSDKVEYKIVHDDPASDDILDNINHNGSALLIEVFTALLRLFIN
EEGDWSCIVVENWIIDDKGVLMERKDERGEGEAKQKRNAHGYFLQDKEKIDNLKDTLK
ENATEVQKESDAKNETNSESDSKSDSDSKERDPKLEKCLNYRNVNWIERLTKRQFNNS
YWLIILLGVLEDCRHLPMYTEFIDSFIEKIIPKDISATQLPKQLWRNFCRKLSFSDKV
NALWILVDLVSHFSPDIKAAVDDSMELCGQIRSERFKVARELKTEAAVLSNLQGDLQA
IQEKLNKTDENTPSADGADKKDDSESNSEPIDLIIIEKKQKLIEEQDKKVQALQSDKN
FLDNCLFENDLQRLKPLGLDRYGNRYFWLDHNGVPFPQYPAGMNETPKSNNSLSYHSG
RLLIQGPKASSAKFFLNVSDEQLSNWQKIRNSEGISEATREVFGISKTSSGSYNYVEN
GIEVELLDSNDRVNPLIELTPIQKKIMDETPSRLLLSPDQWYCIDKLEDLSRIMDWLD
NWGRKEHDLLRQIRPIMERIKSSLSLRDHALSLTAFTKNEEKLLKELENNEFTENELN
VDSMDVDDKNSGVKSEVDVQVDAEEKREAVIDEKLEVIADELMKLDDSSKTRNVLNRI
QELEDQRDELLEQKKSIINSQRPGARILARSERKRTKISRGNKVNKQIEILTDLVNYR
HFKAMEDVIAWKNVLANSIWGSSLRKNASGNKRSGVIETVDDKLKDIVGQTSRTVTPA
PN"
gene complement(264709..268920)
/gene="Snt2"
/locus_tag="R008_G10831"
mRNA complement(264709..268920)
/gene="Snt2"
/locus_tag="R008_G10831"
/product="Snt2p"
CDS complement(264709..268920)
/gene="Snt2"
/locus_tag="R008_G10831"
/note="Similar to YGL131C 'DNA binding protein with
similarity to the S. pombe Snt2 protein; computational
analysis suggests a role in regulation of expression of
genes encoding amine transporters'"
/codon_start=1
/product="Snt2p"
/protein_id="EWG86256.1"
/translation="MPKEEDFQLPRRREAAKNVNYNEMEIDTKLVQQIQIAEKSGAKT
KGSNSQTPRNCKRTSNPASRNEKFKYQKFLHDKNTCWNFIPTLPPSFRKNSRFSNILD
LDDAMIDLKKMSLFNTESVLLSANDTIYMISEPAGEPYYVGRVVNFVSKPEFSNTIHE
AIKTTSVFPAKFFQVRMNWFYRPRDIQEHVNTFNPRLVYASLHQDICPISSYRGKCSI
FHKDEVFDVLPNEKECIIRPNIFYFDELFDRYTLKYYKVYSTDKILNKWNSKSPFLYV
LNRRFRYIYTEPKYPLENVLKKYVFHELEVNELSPADYQWDKRCQFCKEWCIQKESLS
CDECGVCAHLYCMDPPLDRKPNKDVVWTCFSCLQKQQGTKDSHVRFLEEQALELDFIR
SVRQKIEEISSKAIKENVGYNTENCWFQYLGIYSISHIGDALNDSMFFPYPFKPSRVG
VKYQWNGCNHNVPWRRNSYISANSEEERGSTKTSELAWVLDASKITTRKLSEYIEQCK
SEICPILNVRGETCNFIDVVLKNLLFTNYDTAEAFKKCKRELSRKFLKEPSFTAVEIR
KFEEAVEKFGSELRPVCEYVGTQPMSMIVRFYYNWKKTERGLTVRGKLSKLSKNKRKK
EIANHENDVETKYIDDSSFDTEKLSLAESSFQCMFCKTDYSPMWYRVTGGSDDEKIKI
RMQTGVNEKTEISEKSPAHSKKNEKLGALCIRCARMWRRYAIKWVPPLETLRKITGTC
QNSFYSAIEGIIEENNTNKFTLSPFQAHNKLLEWELVQDSELIIRQRMKVYKNPNSFV
KMKRYSMTFHTQLYKMAVRSYRKNEFHPETMQRDLELFIEDNKEVRKAIPEQKPERAK
NTKDEFPVNIIRQSPGTIKTSDTSRNRKCNDVFIEKASNNNIPKITNASNDLIEISIK
TGGSSSGSVSVDKGFKFVKFDNKTFQRLRNSLKLVNNKLPKYNEPSTKKIKMINDIAL
SNPLNEPNGASYNYTVISHSKETSVALEKYHDGNKPSKMLEKDMILKHTKNKPKNPDT
AWANNSARTFCSVCKEKFNDNDNYEVVCGNCGLTVHYFCYAIKLPKDMKKNTNLKTFK
WLCDPCSNDLNPIISTTYQCSMCPTKDYDYDRYRSQSFKICPDALKCTSLGTWVHLVC
SLFNEDIKYGNGQSMQPALNTTAVLIKHSRFTCGVCRINGGGLVKCNKCQYRYHITCA
QNSSNFKLMFEKKNMSVDTTLPCIKDVKLNDTYTLRPILICDRHDISLEGNELYPLSY
KPQHTLSYIEQYCRYYKCESDHSLVELRYFEQLRLRHGEMPGNSHDSAIKPKIYVLPF
ERTCPHCGTNKSLYWYEDIICHSCNLRSGAQELDFDSASANISNDNGLPVEITQQLME
GIEPAMFDIDISEAGTDKNTHPSSQ"
gene 269206..270585
/gene="Ceg1"
/locus_tag="R008_G10836"
mRNA 269206..270585
/gene="Ceg1"
/locus_tag="R008_G10836"
/product="Ceg1p"
CDS 269206..270585
/gene="Ceg1"
/locus_tag="R008_G10836"
/note="Similar to YGL130W 'Alpha (guanylyltransferase)
subunit of the mRNA capping enzyme, a heterodimer (the
other subunit is CET1, an RNA 5'-triphosphatase) involved
in adding the 5' cap to mRNA; the mammalian enzyme is a
single bifunctional polypeptide'"
/codon_start=1
/product="Ceg1p"
/protein_id="EWG86257.1"
/translation="MVLAMESRVAPEIPGLIQPGNVTQDLKMMVCKLLNSPKPTKTFP
GSQPVSFQHSDVEEKLLAHDYYVCEKTDGLRVLMFIVINPVTGEQGCFMIDRENNYYL
VNGFRFPRLPQKKKEELLETLQDGTLLDGELVIQTNPMTKLQELRYLMFDCLAINGRC
LTQSPTSSRLAHLGKEFFKPYFDLRAAYPNRCTTFPFKISMKHMDFSYQLVKVAKSLD
KLPHLSDGLIFTPVKAPYTAGGKDSLLLKWKPEQENTVDFKLILDIPMVEDPSLPKDD
RNRWYYNYDVKPVFSLYVWQGGADVNSRLKHFDQPFDRKEFEILERTYRKFAELSVSD
EEWQNLKNLEQPLNGRIVECAKNQETGAWEMLRFRDDKLNGNHTSVVQKVLESINDSV
SLEDLEEIVGDIKRCWDERRANMAGGSGRPLPSQSQNATLSTSKPVHSQPPSNDKEPK
YVDEDDWSD"
gene complement(270784..272249)
/gene="Rsm23"
/locus_tag="R008_G10841"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(272354..273205)
/gene="Cwc23"
/locus_tag="R008_G10846"
mRNA complement(272354..273205)
/gene="Cwc23"
/locus_tag="R008_G10846"
/product="Cwc23p"
CDS complement(272354..273205)
/gene="Cwc23"
/locus_tag="R008_G10846"
/note="Similar to YGL128C 'Component of a complex
containing Cef1p, putatively involved in pre-mRNA
splicing; has similarity to E. coli DnaJ and other
DnaJ-like proteins and to S. pombe Cwf23p'"
/codon_start=1
/product="Cwc23p"
/protein_id="EWG86258.1"
/translation="MPGHELEDVINQRLNLYDVLELPTPLDVHTIYDDLPQIKRKYRT
LALKYHPDKHPDNPSIIHKFHLLSTATNILTNADVRPHYDRWLIEFLRKTNDIERNKL
IQKLEESESSTIPTTTPHPDLLQIQRHGELLRKLKHFNLPYGDWKHLNTQDQENASQH
PYYDCSTLRIVLDNFLQSNNKSNCLSHLRNQVFITLSANEIYDIYFSERNNYSKDDSI
IIYTVFDTPITAQHVFRNWSSGNLIPTVKDISPLIPLHYYSDFNLETELNDDIARLVS
NEPILLD"
gene complement(273453..273836)
/gene="Soh1"
/locus_tag="R008_G10851"
mRNA complement(273453..273836)
/gene="Soh1"
/locus_tag="R008_G10851"
/product="Soh1p"
CDS complement(273453..273836)
/gene="Soh1"
/locus_tag="R008_G10851"
/note="Similar to YGL127C 'Subunit of the RNA polymerase
II mediator complex; associates with core polymerase
subunits to form the RNA polymerase II holoenzyme;
involved in telomere maintenance; conserved with other
metazoan MED31 subunits'"
/codon_start=1
/product="Soh1p"
/protein_id="EWG86259.1"
/translation="MSSTNGNAPATPSSDQNPLPTRFEVELEFIQSLANIQYVTYLLT
QQQIWKSPNFKNYLKYLEYWCNPPYSQCIVYPNCLFILKLLNGFMESAIVNEDGLLEG
LDELPKIIQLQGPQWMNEMVERWAN"
gene 274058..275200
/gene="Scs3"
/locus_tag="R008_G10856"
mRNA 274058..275200
/gene="Scs3"
/locus_tag="R008_G10856"
/product="Scs3p"
CDS 274058..275200
/gene="Scs3"
/locus_tag="R008_G10856"
/note="Similar to YGL126W 'Protein required for inositol
prototrophy, identified as an ortholog of the FIT family
of proteins involved in triglyceride droplet biosynthesis;
disputed role in the synthesis of inositol phospholipids
from inositol'"
/codon_start=1
/product="Scs3p"
/protein_id="EWG86260.1"
/translation="MSSKWFNAIHLLVCPLTVLVGYLMNAYGYGAALQATLNKDGLVN
AMLVKKGWFWTSLVGWWCIIRYRAVPGATGRDRRHIVQSFKRYAILTVWWYVFTQGIW
FGVGPIMDLVFVYTGGHCHYDVFDDAGHVNEDFQGSVTRTNRALALIHNVLTLHGHHQ
EHRQQQLWDRSIGSIQGALQATQPKTPKNVTASAAAAINTFIHDQMHRWQGPLTTSAQ
CRRFGGHWAGGHDPSGHVFLATLMCMFLLGELRVFGRRALAHLYAQKWQLVRLVTRLF
DTGPLWTWRRCGGGSMTCGARLWRAIVEPPVTCAAALLRLTRCIACDHPVIILLTLLV
TWLWQLLLTAVASRFHTVREHMSGLLAAYIVTGLVYARDAAALRPV"
gene 275574..277376
/gene="Met13"
/locus_tag="R008_G10871"
mRNA 275574..277376
/gene="Met13"
/locus_tag="R008_G10871"
/product="Met13p"
CDS 275574..277376
/gene="Met13"
/locus_tag="R008_G10871"
/note="Similar to YGL125W Major isozyme of
methylenetetrahydrofolate reductase, catalyzes the
reduction of 5,10-methylenetetrahydrofolate to
5-methyltetrahydrofolate in the methionine biosynthesis
pathway"
/codon_start=1
/product="Met13p"
/protein_id="EWG86261.1"
/translation="MKITEKLEQHRQTSGKPTYSFEYFVPKTTQGVQNLYDRMDRMYE
ASLPQFIDITWNAGGGRLSHLSTDLVATAQSVLGLETCMHLTCTNMPISMIDDALENA
YHSGCQNILALRGDPPRDAENWTPVEGGFQYAKDLIKYIKSKYGDHFAIGVAGYPECH
PELPNKDVKLDLEYLKQKIDAGGDFIITQMFYDVDNFINWCSQVRAAGMDVPIIPGIM
PITTYAAFLRRAQWGQISIPQHFSSRLDPIKDDDELVRDIGTNLIVEMCQKLLDSGYV
SHLHIYTMNLEKAPLMILERLNILPMESEFNAHPLAVLPWRKSLNPKRKNEEVRPIFW
KRRPYSYVARTSQWAVDEFPNGRFGDSSSPAFGDLDLCGSDLIRQSANKCLELWSTPT
SINDVAFLVINYLNGNLKCLPWSDIPINDEINPIKAHLIELNQHSIITINSQPQVNGI
RSNDKIHGWGPKDGYVYQKQYLEFMLPKTKLPKLIDTLKNNEFLTYFAIDSQGDLLSN
HPDNSKSNAVTWGIFPGREILQPTIVEKISFLAWKEEFYHILNEWKLNMNKYDKPHSA
QFIQSLIDDYCLVNIVDNDYISPDDQIHSILLSL"
gene complement(277673..279765)
/gene="Mon1"
/locus_tag="R008_G10881"
/note="nonfunctional due to frameshift determined by
sequencing error"
assembly_gap 277702..277953
/estimated_length=252
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 280669..281433
/gene="Rps2"
/locus_tag="R008_G10891"
mRNA 280669..281433
/gene="Rps2"
/locus_tag="R008_G10891"
/product="Rps2p"
CDS 280669..281433
/gene="Rps2"
/locus_tag="R008_G10891"
/note="Similar to YGL123W 'Protein component of the small
(40S) subunit, essential for control of translational
accuracy; phosphorylation by C-terminal domain kinase I
(CTDK-I) enhances translational accuracy; methylated on
one or more arginine residues by Hmt1p'"
/codon_start=1
/product="Rps2p"
/protein_id="EWG86262.1"
/translation="MSAPEAQQQKRGGFGGRNRGRPNRRGPRNTEEKGWVPVTKLGRL
VKAGKITTIEEIFLHSLPVKEFQIIDTLLPGLQDEVMNIKPVQKQTRAGQRTRFKAVV
VVGDSNGHVGLGIKTAKEVAGAIRAGIIIAKLSVIPIRRGYWGTNLGQPHSLATKTTG
KCGSVTVRLIPAPRGSGIVASPAVKKLLQLAGVEDVYTQSNGKTRTLENTLKAAFVAI
GNTYGFLTPNLWAEQPLPVSPLDIYSDEASAQKKRF"
gene complement(281911..283488)
/gene="Nab2"
/locus_tag="R008_G10901"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(283742..284122)
/gene="Gpg1"
/locus_tag="R008_G10906"
mRNA complement(283742..284122)
/gene="Gpg1"
/locus_tag="R008_G10906"
/product="Gpg1p"
CDS complement(283742..284122)
/gene="Gpg1"
/locus_tag="R008_G10906"
/note="Similar to YGL121C 'Proposed gamma subunit of the
heterotrimeric G protein that interacts with the receptor
Gpr1p; involved in regulation of pseudohyphal growth;
requires Gpb1p or Gpb2p to interact with Gpa2p;
overproduction causes prion curing'"
/codon_start=1
/product="Gpg1p"
/protein_id="EWG86263.1"
/translation="MFYLSDIEEEASAGAEPTYNFWEVLLFSNTQENLVTVVGELHTL
TDRVVHYKIEPESREVTATTLPSLLALLLEKRNQARRLYRDVLSMKMSELDWDIDDLF
TQLQEELTRTDDTLSMYPRRRFYH"
assembly_gap 284308..284321
/estimated_length=14
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(284595..286898)
/gene="Prp43"
/locus_tag="R008_G10911"
mRNA complement(284595..286898)
/gene="Prp43"
/locus_tag="R008_G10911"
/product="Prp43p"
CDS complement(284595..286898)
/gene="Prp43"
/locus_tag="R008_G10911"
/note="Similar to YGL120C RNA helicase in the DEAH-box
family, functions in both RNA polymerase I and polymerase
II transcript metabolism, involved in release of the
lariat-intron from the spliceosome"
/codon_start=1
/product="Prp43p"
/protein_id="EWG86264.1"
/translation="MGSKRRFSSEHPDPVETSIPEQAAEIAEELSKQHPLPSEEPLVH
HDAGEFKGLQRHHTSAEEAQKLEDGKINPFTGREFTPKYVDILKIRRELPVHAQRDEF
LKLYQNNQIMVFVGETGSGKTTQIPQFVLFDEMPHLENTQVACTQPRRVAAMSVAQRV
AEEMDVKLGEEVGYSIRFENKTSNKTILKYMTDGMLLREAMEDHDLSRYSCIILDEAH
ERTLATDILMGLLKQVVKRRPDLKIIIMSATLDAEKFQRYFNDAPLLAVPGRTYPVEL
YYTPEFQRDYLDSAIRTVLQIHATEEAGDILLFLTGEDEIEDAVRKISLEGDQLVREE
GCGPLSVYPLYGSLPPHQQQRIFEPAPESHNGRPGRKVVISTNIAETSLTIDGIVYVV
DPGFSKQKVYNPRIRVESLLVSPISKASAQQRAGRAGRTRPGKCFRLYTEEAFQKELI
EQSYPEILRSNLSSTVLELKKLGIDDLVHFDFMDPPAPETMMRALEELNYLACLDDEG
NLTPLGRLASQFPLDPMLAVMLIGSFEFQCSQEILTIVAMLSVPNVFIRPTKDKKRAD
DAKNIFAHPDGDHITLLNVYHAFKSDEAYEYGIHKWCRDHYLNYRSLSAADNIRSQLE
RLMNRYNLELNTTDYESPKYFDNIRKALASGFFMQVAKKRSGAKGYITVKDNQDVLIH
PSTVLGHDAEWVIYNEFVLTSKNYIRTVTSVRPEWLIEIAPAYYDLSNFQKGDVKLSL
ERIKEKVDRLNELKQGKNKKKSKHSKK"
gene 287402..288907
/gene="Abc1"
/locus_tag="R008_G10921"
mRNA 287402..288907
/gene="Abc1"
/locus_tag="R008_G10921"
/product="Abc1p"
CDS 287402..288907
/gene="Abc1"
/locus_tag="R008_G10921"
/note="Similar to YGL119W 'Protein required for ubiquinone
(coenzyme Q) biosynthesis and for respiratory growth;
exhibits genetic interaction with COQ9, suggesting a
common function; similar to prokaryotic proteins involved
in early steps of ubiquinone biosynthesis'"
/codon_start=1
/product="Abc1p"
/protein_id="EWG86265.1"
/translation="MVTNMVKLRNLRRLYCSSRLLRTIQNGRISSVSSISLSKKYTTK
SAKEGEENVERKHEEEKKDTLKSSSVPTSRISRLFHYGSLAAGVGMNAAAKGISEVAK
GNSPTWKSLILSDSNIDRITNKFSKMRGVALKIGQLLSFQDEKVLPKELYEILSRVQN
SANHMPQRQLEKVMAKELGANWKTKFSKFDKIPMAAASIGQVHAAELPSGQRVVVKIQ
YPGVKESIDSDLNSLLMLLTASSLLPKGLFLDKTIANARTELKWECDYNREARALQKF
EALLKDDPAFEVPHVFPEYTTDNIITMTRMEGTEIMKLPKASQETKNFISENIMRLCL
EEIATFKYMQTDPNWANFLYNGRTKKIELLDFGASRPFAEDFILKYRKLLTYATLRDR
KGAYEMSVQLGYLTGLESQSMKDAHVDSVLTLGEPFRGDVDKSFDFKDQTVSDRIRGN
IGLMLNERLCPPPEETYSLHRKFSGIFLLCARMGASVHCAKLFKEIFAYKV"
gene complement(290306..290411)
/locus_tag="R008_G10931"
tRNA complement(290306..290411)
/locus_tag="R008_G10931"
/product="tRNA-Trp"
/note="TW(CCA)G1"
gene 291467..292264
/locus_tag="R008_G10941"
mRNA 291467..292264
/locus_tag="R008_G10941"
/product="hypothetical protein"
CDS 291467..292264
/locus_tag="R008_G10941"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86266.1"
/translation="MQPISIKDVESDQGKVYIVNALKDLVCKCLLEFVDIQIESFMYP
DDPKCFTRIFKGNKIVNEASDKDSKVRSYPSSLGVGHSALFPLIYIRQKTNSLRFLND
PKQLPTPLVDDMNAKFKGIIKVYENLIHLYHSYQTVDCNNMNQQKLLGDLVSRGNFML
DILHGYVTIASTIVRDSKDANILIDTVNRFIHDTILFHKRIIHNSNAYTEYLVMKRGM
QRNQSEETLVELEFRTLDVSDVNLDNEFDDFLQHRKTSLKITHRRVI"
gene 292764..294596
/gene="Cdc20"
/locus_tag="R008_G10946"
mRNA 292764..294596
/gene="Cdc20"
/locus_tag="R008_G10946"
/product="Cdc20p"
CDS 292764..294596
/gene="Cdc20"
/locus_tag="R008_G10946"
/note="Similar to YGL116W 'Cell-cycle regulated activator
of anaphase-promoting complex/cyclosome (APC/C), which is
required for metaphase/anaphase transition; directs
ubiquitination of mitotic cyclins, Pds1p, and other
anaphase inhibitors; potential Cdc28p substrate'"
/codon_start=1
/product="Cdc20p"
/protein_id="EWG86267.1"
/translation="MPESSRDKGNAAISGNRSVLSIASPTKLNILSSDWSRNQGKVSK
NSLKRSSSLNIRNSKRPSLQASANSIYSRPKITIGAPPLIRRDSSFFKDEFDAKKDKA
TFSAYSSRSYPTIGSDSVVSQTSLSQPTTSREVDEQFTVAADRYIPILQGASQNKVDP
ETLHEALPPPNASPISHLRAQTKIVFKQNVAEACGLDMNKRILQYMPEPPKCSSLRQK
SYIMKKRTHYSYQQEQKIPDLIKLRKINTNPERILDAPGFQDDFYLNLLSWSKKNVLA
IALDTALYLWNATTGDVSLLTDFENTTICSVTWSDDDCHISIGKEDGNTEIWDVETMS
LIRTMRSGLGVRIGSLSWLDTLIATGSRSGEIQINDVRIKQHIVSTWAEHTGEVCGLS
YKSDGLQLASGGNDNTVMIWDTRTSLPQFSKKTHSAAVKALSWCPYSPNILASGGGQT
DKHIHFWNSITGARVGSINTGSQVSSLHWGQSHTSTNGGMMNKEIVATGGNPENAISV
YNYETKFKVAEVVHAHEARICCSQLSPDGTTLATVGGDENLKFYKIFDPRCTGRSRED
GLMDGMLGLIGKEGCRTNDKENRSKNSSEIHTRRPSSTSQYLIR"
gene 294987..295955
/gene="Snf4"
/locus_tag="R008_G10951"
mRNA 294987..295955
/gene="Snf4"
/locus_tag="R008_G10951"
/product="Snf4p"
CDS 294987..295955
/gene="Snf4"
/locus_tag="R008_G10951"
/note="Similar to YGL115W 'Activating gamma subunit of the
AMP-activated Snf1p kinase complex (contains Snf1p and a
Sip1p/Sip2p/Gal83p family member); activates
glucose-repressed genes, represses glucose-induced genes;
role in sporulation, and peroxisome biogenesis'"
/codon_start=1
/product="Snf4p"
/protein_id="EWG86268.1"
/translation="MKPTQDSQEKVSIEQQLAVESIRKFLNSKTSYDVLPVSYRLIVL
DTSLLVKKSLNVLLQNSIVSAPLWDSKTSRFAGLLTTTDFINVIQYYFSNPDKFELVD
KLQLDGLKDIERALGVDQLDTASIHPSRPLFEACLKMLESRSGRIPLIDQDEETHREI
VVSVLTQYRILKFVALNCRETHFLKIPIGDLNIITQDNMKSCQMTTPVIDVIQMLTQG
RVSSVPIIDENGYLINVYEAYDVLGLIKGGIYNDLSLSVGEALMRRSDDFEGVYTCTK
NDKLSTIMDNIRKARVHRFFVVDDVGRLVGVLTLSDILKYILLGSN"
gene 296414..298590
/locus_tag="R008_G10956"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 298885..300891
/gene="Sld3"
/locus_tag="R008_G10966"
mRNA 298885..300891
/gene="Sld3"
/locus_tag="R008_G10966"
/product="Sld3p"
CDS 298885..300891
/gene="Sld3"
/locus_tag="R008_G10966"
/note="Similar to YGL113W 'Protein involved in the
initiation of DNA replication, required for proper
assembly of replication proteins at the origins of
replication; interacts with Cdc45p'"
/codon_start=1
/product="Sld3p"
/protein_id="EWG86269.1"
/translation="METWEVIASVKEATKGLDLSLDHPLIIKSEDVPSNILQLLQQKN
RRQLKHICMKSRKEYFLLEEYGPGFWVKWPYNYFNGYSLPERRTEVVTTVERERAKRE
TLKTWDELKFKELLHLWSEEPKGSCKLEKDKDLKLDMNPPDMKGESKINDYYSDPKEY
IESKYYDALFSIHTPLAYFVKSNLVRLKNTCRTKYGSDSYKIAYQAMLQKFLLSIVQF
KDRHDNRLLLEPFSSPIADEKRKNCLTKFVIQDENKNSSTIADLCVVLKSREIKLQIL
LLLEIIGLNDLDWNFRDFEKKYKLKLKKRSLNLTKKGLVRRRSKKKTSEKDKGIERIT
TSLDYCEQLDLYLDRACILDILLSSETPNPDAIEASNGTIQEHKKNILDKSKEASLVG
FINYVLIPYFNKKVPHAVEFIIQKLKGPSMRPKRALKKVNDSTNVSSPNTVETYNRLS
TSQRASRSSIINSVPSSPALRRVDANLFSRKSIASPTPELLNSRTNSNLNEFLESETR
SLKRPSQLGRTKSDLTMNHLQKRQFSVSDLSTTRVPNSSTITLKTPFSHSTINAYKTM
NNSFRRVGKRKDINETIRLHERVDSEENVQVQATPAVKKRTVTPNKKAQLQSIIESPL
NFKDDDTHEGRKNTSNITSTPTNKPPENSSKRRVRRRLFAPEST"
gene complement(301127..302677)
/gene="Taf6"
/locus_tag="R008_G10976"
mRNA complement(301127..302677)
/gene="Taf6"
/locus_tag="R008_G10976"
/product="Taf6p"
CDS complement(301127..302677)
/gene="Taf6"
/locus_tag="R008_G10976"
/note="Similar to YGL112C Subunit (60 kDa) of TFIID and
SAGA complexes, involved in transcription initiation of
RNA polymerase II and in chromatin modification, similar
to histone H4"
/codon_start=1
/product="Taf6p"
/protein_id="EWG86270.1"
/translation="MSTQQQSYTIWSPQDTVKDVAGSLGLENINDDVLKALAMDVEYR
ILEIIEQAVKFKRHSKRDVLTTDDVSKALRVLNVEPLYGYYDGSEVNKAVSFSKVNTS
GGQSVYYLDEEEVDFDRLINEPLPQVPRLPTFTTHWLAVEGVQPAIIQNPNLNDIRVS
QPPFIRGAIVTALNDNSLQTPVTSTTASASVTDTGASQHLSNVKPGQNTEVKPLVKHV
LSKELQIYFNKVISTLTAKSQADEAAQHMKQAALTSLRTDSGLHQLVPYFIQFIAEQI
TQNLSDLQLLTTILEMIYSLLSNTSIFLDPYIHSLMPSILTLLLAKKLGGSPKDDSPQ
EIHEFLERTNALRDFAASLLDYVLKKFPQAYKSLKPRVTRTLLKTFLDINRVFGTYYG
CLKGVSVLEGESIRFFLGNLNNWARLVFNESGITLDNIEEHLNDDSNPTRTKFTKEET
QILVDTVISALLVLKKDLPDLYEGKGEKVTDEDKEKLLERCGVTIGFHILKRDDAKEL
ISAIFFGE"
gene 302927..304317
/gene="Nsa1"
/locus_tag="R008_G10981"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(304485..306359)
/gene="Cue3"
/locus_tag="R008_G10986"
mRNA complement(304485..306359)
/gene="Cue3"
/locus_tag="R008_G10986"
/product="Cue3p"
CDS complement(304485..306359)
/gene="Cue3"
/locus_tag="R008_G10986"
/note="Similar to YGL110C 'Protein of unknown function;
has a CUE domain that binds ubiquitin, which may
facilitate intramolecular monoubiquitination'"
/codon_start=1
/product="Cue3p"
/protein_id="EWG86271.1"
/translation="MLSRYNRVIEINGGNADISLPIVKFPPFKLRAQLIEKDPVVWLH
LIETYVAYFEYLMQGANVELLDESTLDHLRLFLRTYLHEIADEEGKLLSLGINHDVSE
QLYLLKGWIFSLIKKCGLLHLQIFGDSLWNLIKVYVRRNPDSIRGLIDGSLKPRINTQ
RVQLDKSYQVQQHLKQLIESGKFKRIDLRCVEDLLSAKSMQPNKFAENFFTANWIEIL
EALWAKGQGRGHKEARELIIISLFSVSADRLLKITKELGISNFETLALYPLLGTMLIN
EGVHKRLPDLKSKLLFLNLGGLSMDEGDHMSYPISSGTEVNEEQLSALMELFPQFSKY
QLSQTLLAYDNNIELVTNKIFEDPTIIEAFPREPAEEEVEPVSDGDNASFTEELSILD
RGDSSKNKELDKKIISEGVPDELRNKTLTRALKLLYEADEDERDDTYDEADVNRSDPS
KRIGLQEDEESYDTKDDSNEVRQDHNYHIVEAYLWNLLKEDPKLFERSKRGTKVRKTM
KEMTSWSDEKIEGWCRMLERSPTRARLLEKKFMFKGNSKTGKTSYVHNRDSQNDGNVV
KEQAKQKKSENIKKHEPQSTEQKKRQHAKNEKRKGARANHNRKKGHDKKLARAGNNAI
"
gene complement(306596..307018)
/locus_tag="R008_G10996"
mRNA complement(306596..307018)
/locus_tag="R008_G10996"
/product="hypothetical protein"
CDS complement(306596..307018)
/locus_tag="R008_G10996"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86272.1"
/translation="MGLCGSKTQPMPSQTTTVATKARTKPINRDTVKSKQELRHKEKK
DKKKKTQLKSTTVPVVQKKEGSKLTDTSDPSKNKVSPKEAARLAAEKRFQETNEKYNK
GELGKKLAQERAKSHKTRLMEEAEKKHAERERENMIYD"
gene complement(307280..309220)
/gene="Rmd9"
/locus_tag="R008_G11001"
mRNA complement(307280..309220)
/gene="Rmd9"
/locus_tag="R008_G11001"
/product="Rmd9p"
CDS complement(307280..309220)
/gene="Rmd9"
/locus_tag="R008_G11001"
/note="Similar to YGL107C 'Mitochondrial protein required
for respiratory growth; mutant phenotype and genetic
interactions suggest a role in delivering mt mRNAs to
ribosomes; located on matrix face of the inner membrane
and loosely associated with mitoribosomes'"
/codon_start=1
/product="Rmd9p"
/protein_id="EWG86273.1"
/translation="MMLRRNAVRSLKTMEISVSNVVNSGSIAMLRGKLANVVLSDRTY
HSSPIFHKNVPKGVLDKKNGREQRKTEQNVSNVDPASPWRHELLSFDECVSSALKYST
TPLQNTYKRIGNNELNKNPSFAMFWDSMGRAMELYYSLRESPDFNAYRVSRLIHLLHN
GLRSTRDQLVKLSRKPDYDSQSFHKEMMNFLCNSLKDISDDILIGKVSVSGYGATHLL
TSFKELSFDDDCIRIWEASKNLSDETTSQAFQEPKVVGFMLPLLYAKTRSLTEPNELY
NQIIQSKEFIHPNLYSGLIKVFIKAEDYEKALSLFGQLCEKAEVRNYGYLIETHLSFI
GDSKNLTLAESFFDKIINDEMPYKIILQVSTVNSFLQNIWKAQNDFDHVYRIWEKAVK
FYGNTVNPGILSSLNNTFFTIFFENYVNDNINGFRKLQEIITFYSGVKKIDEPFFNVM
LTRASIWHERSIIDFIDKNYTLYHIPRTIISYRILLKSLGSIDNTNNEEILDRWLELV
KKLNELGQQYIANADLSALRDATVVWSQSKRDEKVFSAKAKGTPATTTITEDDIKVPK
PLEDLKNEDSTSNSEDRIELYLKILKRYTPYFRATKQVYRYTTGCAESYPILNEYLSG
YSDLSAEDIPVPQLHSFIAKEQ"
gene 309507..309956
/gene="Mlc1"
/locus_tag="R008_G11006"
mRNA 309507..309956
/gene="Mlc1"
/locus_tag="R008_G11006"
/product="Mlc1p"
CDS 309507..309956
/gene="Mlc1"
/locus_tag="R008_G11006"
/note="Similar to YGL106W 'Essential light chain for
Myo1p, light chain for Myo2p; stabilizes Myo2p by binding
to the neck region; interacts with Myo1p, Iqg1p, and Myo2p
to coordinate formation and contraction of the actomyosin
ring with targeted membrane deposition'"
/codon_start=1
/product="Mlc1p"
/protein_id="EWG86274.1"
/translation="MSATRANKDIFTLFDKKGQGAIAKDSLGDYLRAIGYNPTNQLVQ
DIINADSSLRDASSLTLDQITGLIEVNEKELDATTKAKTEDFVKAFQVFDKESTGKVS
VGDLRYMLTGLGEKLTDAEVDELLKGVEVDSNGEIDYKKFIEDVLRQ"
gene 310404..311534
/gene="Arc1"
/locus_tag="R008_G11011"
mRNA 310404..311534
/gene="Arc1"
/locus_tag="R008_G11011"
/product="Arc1p"
CDS 310404..311534
/gene="Arc1"
/locus_tag="R008_G11011"
/note="Similar to YGL105W 'Protein that binds tRNA and
methionyl- and glutamyl-tRNA synthetases (Mes1p and
Gus1p), delivering tRNA to them, stimulating catalysis,
and ensuring their localization to the cytoplasm; also
binds quadruplex nucleic acids'"
/codon_start=1
/product="Arc1p"
/protein_id="EWG86275.1"
/translation="MSDLVTKFESLIISKYPVSFTKEQSAQAAQWESVLKSGQIQPHL
DQLNLVLRDNTFIVSTLYPTSTDVHVFEVALPLIKDLVASSKDVKSTYTTYRHILRWI
DYMQNLLEVSSTDKLEINHDLDLPHEVIEKKKKAPAGGAADAAAKADEDVSKKAKKQD
HPRGKPDEETLKKLREEAKAKKAAKKAANAKQQQEQQNKAPEKPKPSAIDFRVGFIQK
AIKHPDADSLYVSTIDVGDEEGPRTVCSGLVKHFPLDAMQERYVVVVCNLKPVNMRGI
KSTAMVLCGSNDDKVEFVEPPKDSKAGDKVFFEGFGDEAPMKQLNPKKKIWEHLQPHF
TTNDGLEVIFKDEEEKDHPVRKLTNAKGESFKVASIANAQVR"
gene complement(311680..313140)
/gene="Vps73"
/locus_tag="R008_G11016"
mRNA complement(311680..313140)
/gene="Vps73"
/locus_tag="R008_G11016"
/product="Vps73p"
CDS complement(311680..313140)
/gene="Vps73"
/locus_tag="R008_G11016"
/note="Similar to YGL104C 'Mitochondrial protein; mutation
affects vacuolar protein sorting; putative transporter;
member of the sugar porter family'"
/codon_start=1
/product="Vps73p"
/protein_id="EWG86276.1"
/translation="MNRILSSASLLSNVSMPRQNKHKITKALCYAIIVASIGSIQFGY
HLSELNAPQQVLSCSEFDIPMEGYPYDRTWLGKRGYKQCIPLNDEQIGIVTSVFCIGG
ILGSYFATSLANIYGRKFSSLINCTLNIVGSLIIFNSNSYRGLIIGRILVGISCGSLI
VIIPLFIKEVAPSGWEGLLGSMTQICIRLGVLLTQGIALPLTDSYRWRWILFGSFLIA
VLNFFMWFIVDESPKWLLAHGRVTDAKLSLCKLRGVTFDEAAQEIQDWQLQIESGDPL
IEPTTTNSISGYNSLWKYLRDRTNVKSRHVITVLLFGQQFCGINSIVLYGTKIISQLY
PQHAIRINFFISMVNVLVTILVSLLIHSLPRKPLLMTSTVLVSVTAFIMGIAMNHNKM
NLLIVFSFIYMGVFTMGLNPLPFIIMREVSKPQDMVLAQRYGTICNWVGTFIIAYTFP
IIHDVLSGYVFIIFAIIACSISAFIWKKVPETKRSG"
gene 313933..314894
/gene="Rpl28"
/locus_tag="R008_G11026"
mRNA join(313933..313981,314494..314894)
/gene="Rpl28"
/locus_tag="R008_G11026"
/product="Rpl28p"
CDS join(313933..313981,314494..314894)
/gene="Rpl28"
/locus_tag="R008_G11026"
/note="Similar to YGL103W 'Ribosomal protein of the large
(60S) ribosomal subunit, has similarity to E. coli L15 and
rat L27a ribosomal proteins; may have peptidyl transferase
activity; can mutate to cycloheximide resistance'"
/codon_start=1
/product="Rpl28p"
/protein_id="EWG86277.1"
/translation="MPSRFTKTRKHRGHVSAGKGRIGKHRKHPGGRGMAGGQHHHRIN
MDKYHPGYFGKVGMRYFHKQQAHFWKPVLNLDKLWTLIPEDKRDQYLKSASKETAPVI
DTLAAGYGKILGKGRIPNVPVIVKARFVSKLAEEKIRAAGGVVELIA"
gene 315160..315807
/locus_tag="R008_G11036"
mRNA 315160..315807
/locus_tag="R008_G11036"
/product="hypothetical protein"
CDS 315160..315807
/locus_tag="R008_G11036"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86278.1"
/translation="MTAVNIWKPEDNIPREILAILSKPHPNYQLAFLNIIQLLKTQRR
TGWVDHGIDPCESISDHMYRMGLTTMLITDKNVDRNKCIRIALVHDFAESLVGDITPN
DPMTKEEKHRREFETVKYLCESIIRPCSESASREILDDWLAYEKQTCLEGRYVKDIDK
YEMLVQCFEYEQKYNGKKDLKQFWGAINDIKTDEVKKWTQSLLEDRQAFFDSLKE"
gene 316201..317250
/gene="Seh1"
/locus_tag="R008_G11041"
mRNA 316201..317250
/gene="Seh1"
/locus_tag="R008_G11041"
/product="Seh1p"
CDS 316201..317250
/gene="Seh1"
/locus_tag="R008_G11041"
/note="Similar to YGL100W 'Nuclear pore protein of the
conserved Nup84p complex (Nup84p, Nup85p, Nup120p,
Nup145p, and Seh1p); part of the SEA (Seh1-associated)
complex, a coatamer-related complex that associates
dynamically with the vacuole; homologous to Sec13p'"
/codon_start=1
/product="Seh1p"
/protein_id="EWG86279.1"
/translation="MQPFDSGHDDLVHDVVYDFYGRHVATCSSDQHIKVFKLDKDTSN
WELSDSWRAHDSSIVAIDWASPEYGRIIASASYDKTVKLWEEDPDQEECSGRRWNKLC
TLNDSKGSLYSVKFAPAHLGLKLACLGNDGILRLYDALEPSDLRSWTLTSEMKVLSIP
PANHLQSDFCLSWCPSRFSPEKLAVSALEQAIIYQRGKDGKLHVAAKLPGHKSLIRSI
SWAPSIGRWYQLIATGCKDGRIRIFKITEKLSPLASEESLTNSNMFDNGADVDMDAQG
RSDSNTEEKAELQSNLQVELLSEHDDHNGEVWSVSWNLTGTILSSAGDDGKVRLWKAT
YSNEFKCMSVITAQQ"
gene 317598..319520
/gene="Lsg1"
/locus_tag="R008_G11046"
mRNA 317598..319520
/gene="Lsg1"
/locus_tag="R008_G11046"
/product="Lsg1p"
CDS 317598..319520
/gene="Lsg1"
/locus_tag="R008_G11046"
/note="Similar to YGL099W 'Putative GTPase involved in 60S
ribosomal subunit biogenesis; required for the release of
Nmd3p from 60S subunits in the cytoplasm'"
/codon_start=1
/product="Lsg1p"
/protein_id="EWG86280.1"
/translation="MPPKEAPKKWKAPKGPKPTHRKNKNKLELGRAIKYARQKENAIE
YLPDGEMRFTTDKHEANWVKLRSVTQESALDEFLSTAALADKDFTADRHSNVKIIRMD
SGNDSATSQGFSMTNEQRGNLNAKQRALTKDLIVPRRPEWNEGMSKFQLDRQEKEAFL
EWRRKLAHLQESNEDLLLTPFERNIEVWKQLWRVVERSDLVVQIVDARNPLLFRSVDL
ERYVKESDNRKANLLLVNKADLLTKKQRIAWAKYFISKNISFTFYSALRANQLLEKQK
EMGEDYREQDFEEADEEGFDADEKVMEKVKILSIDQLEELFLSKAPNEPLLPPLPGQP
PLINIGLVGYPNVGKSSTINSLVGAKKVSVSSTPGKTKHFQTIKLSDSVMLCDCPGLV
FPNFAYNKGELVCNGVLPIDQLRDYIGPAGLVAERIPKYYIEAIYGIHIQTKSRDEGG
NGDIPTAQELLVAYARARGYMTQGYGSADEPRASRYILKDYVNGKLLYVNPPPHLEDD
TPYTREECEEFNKDLYVFDRLPDTRKEQVQNAAKAKGIDIVDLARDLNQLTFSAHTGG
DTQKEAKSVTHGGKQAALYNAAEDLDRDFFKMNNVEGRLSTPFHKVQNSSAGKRHNKK
NKSKNAKSKVFSIENN"
gene 319755..320022
/locus_tag="R008_G11051"
ncRNA 319755..320022
/ncRNA_class="snoRNA"
/locus_tag="R008_G11051"
/product="SNR82"
gene 320309..321046
/gene="Use1"
/locus_tag="R008_G11056"
mRNA 320309..321046
/gene="Use1"
/locus_tag="R008_G11056"
/product="Use1p"
CDS 320309..321046
/gene="Use1"
/locus_tag="R008_G11056"
/note="Similar to YGL098W 'Essential SNARE protein
localized to the ER, involved in retrograde traffic from
the Golgi to the ER; forms a complex with the SNAREs
Sec22p, Sec20p and Ufe1p'"
/codon_start=1
/product="Use1p"
/protein_id="EWG86281.1"
/translation="MAETSNDPFLSYVLSSKQLTNLNRLRRKAVTKQLGSSDDNKVSE
EFLRYQHTYQREAFEYLQTKHDAHKIMESQYEQYQSSSKTRRYSIDLDSVDAVDTESQ
TEYPNEEFIDRSEDSEAVMELRKRLLGKGQNKGLGYETTKSVDRQIEDQDTLQQDLIQ
DMSKLVGSLKQGAVAFQSALDEDKQVLGAAEIGIQVASQGLMDVSGKLRKYDKSKLSY
LFYITVFIFMILGLVFTFIIIQLFPAL"
gene 322758..322829
/locus_tag="R008_G11061"
tRNA 322758..322829
/locus_tag="R008_G11061"
/product="tRNA-His"
/note="TH(GUG)G2"
gene 324771..326219
/gene="Srm1"
/locus_tag="R008_G11066"
mRNA 324771..326219
/gene="Srm1"
/locus_tag="R008_G11066"
/product="Srm1p"
CDS 324771..326219
/gene="Srm1"
/locus_tag="R008_G11066"
/note="Similar to YGL097W 'Nucleotide exchange factor for
Gsp1p, localizes to the nucleus, required for
nucleocytoplasmic trafficking of macromolecules;
suppressor of the pheromone response pathway; potentially
phosphorylated by Cdc28p'"
/codon_start=1
/product="Srm1p"
/protein_id="EWG86282.1"
/translation="MVKRTVATNGDASGAHRAKKMSKTHASHIINAQEDYKHMYLSVQ
PLDIFCWGTGSMCELGLGPQAKNKEVKRPRLNPFLPRDEAKIISFAVGGMHTLALDEE
SNVWSWGCNDVGALGRDTSNAKEQLKDMDADDSSDDEDGDLNELESTPAKIPRESFPP
LAEGHKVVQLAATDNMSCALFSNGEVYAWGTFRCNEGILGFYQDKIKIQKTPWKVPTF
SKYNIVQLAPGKDHILFLDEEGMVFAWGNGQQNQLGRKVMERFRLKTLDPRPFGLRHV
KYIASGENHCFALTKDNKLVSWGLNQFGQCGVSEDVEDGALVTKPKRLALPDNVVIRS
IAAGEHHSLILSQDGDLYSCGRLDMFEVGIPKDNLPEYTYKDVHGKARAVPLPTKLNN
VPKFKSVAAGSHHSVAVAQNGIAYSWGFGETYAVGLGPFEDDTEVPTRIKNTATQDHN
IILVGCGGQFSVSGGVKLSDEDAEKRADEMDD"
gene 328326..329156
/gene="Tos8"
/locus_tag="R008_G11081"
mRNA 328326..329156
/gene="Tos8"
/locus_tag="R008_G11081"
/product="Tos8p"
CDS 328326..329156
/gene="Tos8"
/locus_tag="R008_G11081"
/note="Similar to YGL096W 'Homeodomain-containing protein
and putative transcription factor found associated with
chromatin; target of SBF transcription factor; induced
during meiosis and under cell-damaging conditions; similar
to Cup9p transcription factor'"
/codon_start=1
/product="Tos8p"
/protein_id="EWG86283.1"
/translation="MGTSIVNLNQKIELPPIQVLFESLNRENETKPHFEERRLYQPNP
SFVPRTNIAVGSPVNPVPVSSPVFFIGPSPQRSIQNHNAIMTQNIRQYPVIYNNNREV
ISTGERNYIITVGGPPVTSSQPEYEHISTPNFYQEQRLAQPHPVNESMMIGGYTNPQP
ISISRGKMLSGNISTNSVRGSNNGYSAKEKKHKAHGKRSNLPKATVSILNKWLHEHVN
NPYPTVQEKRELLAKTGLTKLQISNWFINARRKKIFSGQNDANNFRRKFSSSTNLAKF
"
assembly_gap 329214..329265
/estimated_length=52
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 331577..331648
/locus_tag="R008_G11086"
tRNA 331577..331648
/locus_tag="R008_G11086"
/product="tRNA-Glu"
/note="TE(UUC)G1"
gene complement(331868..333601)
/gene="Vps45"
/locus_tag="R008_G11091"
mRNA complement(331868..333601)
/gene="Vps45"
/locus_tag="R008_G11091"
/product="Vps45p"
CDS complement(331868..333601)
/gene="Vps45"
/locus_tag="R008_G11091"
/note="Similar to YGL095C 'Protein of the Sec1p/Munc-18
family, essential for vacuolar protein sorting; required
for the function of Pep12p and the early endosome/late
Golgi SNARE Tlg2p; essential for fusion of Golgi-derived
vesicles with the prevacuolar compartment'"
/codon_start=1
/product="Vps45p"
/protein_id="EWG86284.1"
/translation="MNLFDVADFYINKIVTSQSKLSVANVNEHQRIKVLLLDKNTTPT
ISLCATQSELLKHEIYLVERIENEQREVSRHLRCLVYVKPTEETLQHLLRELRNPRYG
EYQIFFSNIVSKSQLERLAESDDLEAVTKVEEIFQDFFILNQDLFSFDLQPREFLSNK
LVWSEGGLTKCTNSLVSVLLSLKIKPDIRYEGASKICERLAKEVSYEIGKNERTFFDF
PVMDSTPVLLILDRNTDPITPLLQPWTYQSMINEYIGIKRNIVDLSKVPRIDKDLEKV
TLSSKQDAFFRDTMYLNFGELGDKVKQYVTTYKDKTQTNSQINSIEDIKNFIEKYPEF
RKLSGNVAKHMAIVGELDRQLKIKNIWEISEIEQNLSAHDANEEDFSDLIKLLQNEAV
DKYYKLKLACIYSLNNQTSSDKIRQLVEILSQQLPPEDVNFFHKFKSLFSRQDKMTQS
NHDKDDILTELARRFNSRMNSKSNTAENVYMQHIPEISSLLTDLSKNALSRDRFKEID
TQGHRVIGNQQSKDIPQDVILFVIGGVTYEEARLVHDFNGTMNNRMRVVLGGTSILST
KEYMDSIRSAK"
gene complement(334112..337459)
/gene="Pan2"
/locus_tag="R008_G11096"
mRNA complement(334112..337459)
/gene="Pan2"
/locus_tag="R008_G11096"
/product="Pan2p"
CDS complement(334112..337459)
/gene="Pan2"
/locus_tag="R008_G11096"
/note="Similar to YGL094C Essential subunit of the
Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to
control poly(A) tail length and regulate the stoichiometry
and activity of postreplication repair complexes"
/codon_start=1
/product="Pan2p"
/protein_id="EWG86285.1"
/translation="MNNWQHFFNNPVDLSEHLKKPYFRFDNRDKEITAISFDEKANLI
WSGDSYGCISSYDPTFQLYTRYRGHIGGNSVKDILSHRDGILSISEDSLHFANRRGVT
KLNLTSIDIAAFSELNTMCYSPHSLKNNIYCGGDNTNWGIASIDLNRGCLDSLLNYSS
KVKLMCSNNKVLSIGRQTGTVDLLDPTSNRTIKSFNAHSASISAMDLRDNTLVTVGKS
KRFYNLYADPFVNVYDLRTMRQLPPVSFSKGTTMGSGGADFVQLHPLLPTVMIVASSS
GSFDFIDLSNPTLRTQYVHPCQSIKKLCLSPNGDVLGILEADNHLDTWRRSSNNMGMF
TNTPEMLAYPDYFNDITSDGPISVDDETYPLSSVGMPYYLDKLLSAWPPVVFKSEGTI
PQLTGKSPLPSSGKLKSNLAVISSQNEKLSTQEFPLLRYDRTKYGMRNAIPDYVCLRD
IRKQITSGLETSDIQTYTSINKYEVPPAYSRLPLTSGRFGTDNFDFTPFNNTEYSGLD
PDVDNHYTNAIIQLYRFIPEMFNFVVGCLKDENFETTLLTDLGYLFDMMERSHGKICS
SSNFQASLKSLTDKRQLENGEPQEHLEEYLESLCIRESIEDFNSSESIKRNMPQKFNR
FLLSQLIKEEAQTVNHNITLNQCFGLETEIRTECSCDHYDTTVKLLPSLSISGINKTV
IKQLNKKSNGQNILPYIEYAMKNVTQKNSICPTCGKTETITQECTVKNLPSVLSLELS
LLDTEFSNIRSSKNWLTSEFYGSIIKNKAVLRSTASELKGTSHIFKYELNGYVAKITD
NNNETRLVTYVKKYNPKENCFKWLMFNDYLVVEITEEEALKMTYPWKTPEIIIYCDAE
ELRKPFFSVDTYSINYDILFRDYFANGIRDTARREYKLLTHDEAPKSGTLVAIDAEFV
SLQSELCEIDHQGIRSIIRPKRTALARISIIRGEEGELYGVPFVDDYVVNTNHIEDYL
TRYSGILPGDLDPEKSTKRLVRRNVVYRKVWLLMQLGCVFVGHGLNNDFKHININVPR
NQIRDTAIYFLQGKRYLSLRYLAYVLLGMNIQEGNHDSIEDAHTALILYKKYLHLKEK
AIFEKVLNSVYEEGRAHNFKVPETSKG"
gene 337880..340633
/gene="Spc105"
/locus_tag="R008_G11101"
mRNA 337880..340633
/gene="Spc105"
/locus_tag="R008_G11101"
/product="Spc105p"
CDS 337880..340633
/gene="Spc105"
/locus_tag="R008_G11101"
/note="Similar to YGL093W 'Subunit of a
kinetochore-microtubule binding complex with Kre28p that
bridges centromeric heterochromatin and kinetochore MAPs
and motors; required for sister chromatid bi-orientation
and kinetochore binding of SAC components'"
/codon_start=1
/product="Spc105p"
/protein_id="EWG86286.1"
/translation="MNVDERSRIGGREKDAGPGKGILKQNQSSQMTSSFLENPGVRIP
TRIITKKEVLDGSNTTSRINTSNLQSMVKRRVSFAPDVTLHSFTFVPEQNNEIKEPRR
RKTSTNSPTKISSQEEPLVTSTQIDDARTEEKTAAEEDPDTSGMELTEPIVATPDSNK
ASQHDPTSMEMTEVFPRSIRQKNPDVEGESIESSQQIDDVEAVREETMELTAIHNVHD
YDSISKDTVEGEPIDLTEYESKPYVPNSVSRSTGKSSDYSVERSNDKSDLSKSENKTN
SSQPMEITDIFHADPQNPMSLHSDNNINNDGNEMELTQIQTNFDRDNHHIDESPSEKH
AFSSNKRRKLDTVSDYAASVTTPVKEAKDTSGEDNDGDLEMMEKMSPITFSDVDNKIG
TRSNDVFTIEPGTEDTGMQTATDDEEDGENVDDNGNKIVEKTRLPEIDKEGQSGIALP
TQDYTLREFINEVGVGFLDTKLIDDLDKKVNFPLNSFNFVENQRIDNVFSAFYIDIPI
LEVEAFRCKELWRSINESKDKFKDFEAQIDKSHPPLLLQEYFSSDEKMKQLMRDQLQL
VKGYSKLEAAMEWYEWRKKQLNGLELILAENLNTLKREYEKLNEEVEKVNSIRGKIRK
LNEAIKEEIRSLKNLPSDSYKPTLMNRIKIEAFKQELMEHSISLSSSNDFTQEMRSLK
LAIAKKSNDILTLRSEVASIDKKIEKRKLFTRFDLPKLRDTLKILESLTGVRFLKFSK
ATLSIAFLQLDDLRVDINLANFKNNPLSSMKVMNDSNNDDMSYHLFTMLLKNVEAEHQ
DSMLSNLFFAMKKWRPLLKYIKLLKLLFPVKITQTEEEEALLQFKDYDRRNKTAFFYV
ISLVSFAQGVFSENGQIPMKVHISTQQDYSPSREVLSDRITHKISGVLPSFTKSRIHL
EFT"
gene 340900..344886
/gene="Nup145"
/locus_tag="R008_G11106"
mRNA 340900..344886
/gene="Nup145"
/locus_tag="R008_G11106"
/product="Nup145p"
CDS 340900..344886
/gene="Nup145"
/locus_tag="R008_G11106"
/note="Similar to YGL092W Essential nucleoporin, catalyzes
its own cleavage in vivo to generate a C-terminal portion
that assembles into the Nup84p subcomplex of the nuclear
pore complex, and an N-terminal portion of unknown
function that is homologous to Nup100p"
/codon_start=1
/product="Nup145p"
/protein_id="EWG86287.1"
/translation="MFNKSVNSGFTFGNQNTSTPTSTPAQPSSSLQFPQKSTGLFGNV
NVNANTSTPSPSGGLFNANSNANSISQQPANNSLFGNKPAQPSGGLFGATNNTTSKSA
GSLFGNNNATANSTGSTGLFGNNHNSSGGLFSGSNNIASSTQNGGLFGNSNNNNITST
TQNGGLFGKPTTTPAGAGGLFGNSSSTNSTTGLFGSNNTQSSTGIFGQKPGASTTGGL
FGNNGASFPRSGETTGTMSTNPYGINISNVPMAVADMPRSITSSLSDVNGKSDAEPKP
IENRRTYSFSSSVSGNAPLPLASQSSLVSRLSTRLKATQKSTSPNEIFSPSYSKPWLN
GAGSAPLVDDFFSSKMTSLAPNENSNFPQNVFNFLSSQRADLTELRKLKIDSNRSAAK
KLKLLSGTPAITKKHMQDEQDSSKNEPIANADSVTNIDRKENRDNNLDNTYLNGKEQS
NNLNKQDGENTLQHEKSSSFGYWCSPSPEQLERLSLKQLAAVSNFVIGRRGYGCITFQ
HDVDLTAFTKSFREELFGKIVIFRSSKTVEVYPDEATKPMIGHGLNVPAIITLENVYP
VDKKTKKPMKDTTKFAEFQVFDRKLRSMREMNYISYNPFGGTWTFKVNHFSIWGLVNE
EDAEIDEDDLSKQEDGGEQPLRKVRTLAQSKPSDKEVILKTDGTFGTLSGKDDSIVEE
KAYEPDLSDADFEGIEASPKLDVSKDWVEQLILAGSSLRSVFATSKEFDGPCQNEIDL
LFSECNDEIDNAKLIMKERRFTASYTFAKFSTGSMLLTKDIVGKSGVSIKRLPTELQR
KFLFDDIYLDKEIEKVTIEARKSNPYPQISESSLLFKDALDYMEKTSSDYNLWKLSSI
LFDPVSYPYKTDNDQVKMALLKKERHCRLTSWIVSQIGPEIEEKIRNSSNEIEQIFLY
LLLNDVVRASKLAIESKNGHLSVLISYLGSNDPRIRDLAELQLQKWSTGGCSIDKNIS
KIYKLLSGSPFEGLFSLKELESEFSWLCLLNLTLCYGQIDEYSLESLVQSHLDKFSLP
YDDPIGVIFQLYAANENTEKLYKEVRQRTNALDVQFCWYLIQTLRFNGTRVFSKETSD
EATFAFAAQLEFAQLHGHSLFVSCFLNDDKAAEDTIKRLVMREITLLRASTNDHTLNR
LKIPSQLIFNAQALKDRYEGNYLSEVQNLLLGSSYDLAEMAIVTSLGPRLLLSNNPVQ
NNELKTLREILNEFPDSERDKWSVSINVFEVYLKLVLDNVETQETIDSLISGMKIFYD
QYKHCREVAACCNVMSQEIVSKILEKNNPSIGDSKAKLLELPLGQPEKAYLRGEFAQD
LMKCTYKI"
gene complement(345082..346068)
/gene="Nbp35"
/locus_tag="R008_G11111"
mRNA complement(345082..346068)
/gene="Nbp35"
/locus_tag="R008_G11111"
/product="Nbp35p"
CDS complement(345082..346068)
/gene="Nbp35"
/locus_tag="R008_G11111"
/note="Similar to YGL091C 'Essential iron-sulfur cluster
binding protein localized in the cytoplasm; forms a
complex with Cfd1p that is involved in iron-sulfur protein
assembly in the cytosol; similar to P-loop NTPases'"
/codon_start=1
/product="Nbp35p"
/protein_id="EWG86288.1"
/translation="MTEILPHVNDEVLPAEYELNQPEPEHCPGPESDMAGKSDACGGC
ANKEICESLPKGPDPDIPLITDNLSGIEHKILVLSGKGGVGKSTFAAMLSWALSADED
LQVGAMDLDICGPSLPHMLGCIKETVHESNSGWTPVYVTDNLATMSIQYMLPEDDSAI
IWRGSKKNLLIKKFLKDVDWDKLDYLVIDTPPGTSDEHISINKYMRESGIDGALVVTT
PQEVALLDVRKEIDFCKKAGINILGLVENMSGFVCPNCKGESQIFKATTGGGEALCKE
LGIKFLGSVPLDPRIGKSCDMGESFLDNYPDSPASSAVLNVVEALRDAVGDV"
gene 346345..347610
/gene="Lif1"
/locus_tag="R008_G11126"
mRNA 346345..347610
/gene="Lif1"
/locus_tag="R008_G11126"
/product="Lif1p"
CDS 346345..347610
/gene="Lif1"
/locus_tag="R008_G11126"
/note="Similar to YGL090W 'Component of the DNA ligase IV
complex that mediates nonhomologous end joining in DNA
double-strand break repair; physically interacts with
Dnl4p and Nej1p; homologous to mammalian XRCC4 protein'"
/codon_start=1
/product="Lif1p"
/protein_id="EWG86289.1"
/translation="MSQLTEFISCIPVVNEEQNEEDERGLCKIQIEDGAMLETLDENS
LSGLRIEKMLVSEGTGIFSKSSFGINDLRIFTGENIDEESKKYVWYELLKMLTGHKVY
IASLDEKVVFTKWTCRMQDDEVWKVVMELESSAIIRKIAELTLHPVKKGEIDLFEMAD
KLYKDICCVNDSYRNIKESDSSNRNRVEQLARERELLDKLLETRDERTRAMMVTLLNE
KKKKIRELHEILRQNNIKLSDDDVLDSALINTEVQKPISELNSPGKRMKRRKTVVEPQ
NLQKKLKDTSRRRANRKISNQSVIKMEDDDFDDFQFFGLSKRPIITAKDKLSEKYDDI
TSFGDDTQSISFESDSSSDVQKHLVSLEDNGIQISAGRSDEDYGDISGSESETDASAG
EKKSSNHSEQSGNDREPCLQTESETDIET"
gene complement(347816..348178)
/gene="Mf(alpha)2"
/locus_tag="R008_G11131"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 349011..349255
/locus_tag="R008_G11141"
ncRNA 349011..349255
/ncRNA_class="snoRNA"
/locus_tag="R008_G11141"
/product="SNR10"
gene complement(349431..349929)
/gene="Mms2"
/locus_tag="R008_G11146"
mRNA complement(join(349431..349833,349919..349929))
/gene="Mms2"
/locus_tag="R008_G11146"
/product="Mms2p"
CDS complement(join(349431..349833,349919..349929))
/gene="Mms2"
/locus_tag="R008_G11146"
/note="Similar to YGL087C 'Ubiquitin-conjugating enzyme
variant involved in error-free postreplication repair;
forms a heteromeric complex with Ubc13p, an active
ubiquitin-conjugating enzyme; cooperates with
chromatin-associated RING finger proteins, Rad18p and
Rad5p'"
/codon_start=1
/product="Mms2p"
/protein_id="EWG86290.1"
/translation="MSKVPRNFRLLEELEKGEKGFGPESCSYGLADSDDITMTKWNGT
ILGPPHSNHENRIYSLSIDCGPNYPDSPPKVTFISKINLPCVNPTTGEVQTDFHTLRD
WKRAYTMETLLLDLRKEMATPANKKLRQPKEGETF"
gene 350144..352426
/gene="Mad1"
/locus_tag="R008_G11151"
mRNA 350144..352426
/gene="Mad1"
/locus_tag="R008_G11151"
/product="Mad1p"
CDS 350144..352426
/gene="Mad1"
/locus_tag="R008_G11151"
/note="Similar to YGL086W 'Coiled-coil protein involved in
the spindle-assembly checkpoint; phosphorylated by Mps1p
upon checkpoint activation which leads to inhibition of
the activity of the anaphase promoting complex; forms a
complex with Mad2p'"
/codon_start=1
/product="Mad1p"
/protein_id="EWG86291.1"
/translation="MDVRAALQCFFSALSGRFTGKKLGLEIYSIQYKMSNSGGSSPFL
ESPGGSPDVGSTNGQSNRQIQALQFKLNTLQNEYEIEKLQLQKQTNILEKKYKATIDE
LEKALNDTKYLYESNDKLEQELKSLKERSANSMNDKDKCIEELRTTLQNKDLEMETLR
QQYDSKLSKVTNQCDHFKLEAESSHSLLMKYEKEIKRQSVDIKDLQHQVMEKDDELSS
VKASKMINSHPNYSTEEFNELTEMNKMIQDQVQYTKELELANMQQANELKKLKQSQDT
STFWKLENEKLQNKLSQLHVLESQYENLQLENIDLKSKLTKWEIYNDSDDDDDNNVNN
NDNNNNNKNDNNNDNNNDTSNNNNINNNDTSNNNNINNNNRTKNNIRNNPEEIIRDWK
LTKKECLILTDMNDKLRLDNNNLKLLNDEMALERNQILDLNKNYENNIVNLKRLNHEL
EQQKSLSFEECRLLREQLDGLYSAQNNALLEVENSETHASNKNVNEDMNNLIDTYKNK
TEDLTNELKKLNDQLLSNSNDVETQRKKRKLTSDQIGLNYSQRLNELQLENVSVSREL
SKAQTTIQLLQEKLEKLTKLKEKKIRILQLRDGPFIKDQFIKKNKLRLLEKENADLLN
ELKKNNPAVETVPISVYDSLNFELKQFEQEVFKSNKRFSRLKQVFNNKSLEFIDVVNS
LLGFKLEFQQDSRVKIFSCFKPEKYLIADLNENTLKSNLDADIEGWDDLMNLWVEDRG
QLPCFLATITLRLWEQRQAK"
gene 352674..353498
/locus_tag="R008_G11156"
mRNA 352674..353498
/locus_tag="R008_G11156"
/product="hypothetical protein"
CDS 352674..353498
/locus_tag="R008_G11156"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86292.1"
/translation="MREGDSNSKKSADVAVLSIILTGSTLTLIYTYKRYLTQFKRTND
IPRRIFRKHWLYGKVTSVGDGDNFHFFHMPGGIRGGWGWLRPVPQMIKNDSTAEKLVG
DSRNMRFFNFNWITHGRSTKSKIQKAKSQFLKLNVPYKNRKNLPTIPIRLCGIDAPER
AHFGNPAQPFGNEALIWLQNRILGKKVWVKPLSIDQYNRCVARVSYWDWFGGWKDLSL
EMLKDGLAVVYEGKVNTEFDDREDKYRYYEFLARSRKKGLWIQNKFETPGEYKKRI"
gene complement(353674..355356)
/gene="Gup1"
/locus_tag="R008_G11161"
mRNA complement(353674..355356)
/gene="Gup1"
/locus_tag="R008_G11161"
/product="Gup1p"
CDS complement(353674..355356)
/gene="Gup1"
/locus_tag="R008_G11161"
/note="Similar to YGL084C 'Plasma membrane protein
involved in remodeling GPI anchors; member of the MBOAT
family of putative membrane-bound O-acyltransferases;
proposed to be involved in glycerol transport'"
/codon_start=1
/product="Gup1p"
/protein_id="EWG86293.1"
/translation="MSLISILSPLITSEGLDSRIKPSPKKDASTTTKPSLWKTTEFKF
YYIAFLVVVPLMFYAGLQASSPENPNYARYERLLSQGWLFGRKVDNSDSQYRFFRDNF
ALLSVLMLVHTSIKRIVLYSTNITKLRFDLIFGLIFLVAAHGVNSIRILAHMLILYAI
AHVLKNFRRIATISIWIYGISTLFINDNFRAYPFGNICSFLSPLDHWYRGIIPRWDVF
FNFTLLRVLSYNLDFLERWENLQKKKSPSYESKEAKSAILLNERGRLTAAHPIQDYSL
MNYIAYVTYTPLFIAGPIITFNDYVYQSKHTLPSINFKFIFYYAVRFVIALLSMEFIL
HFLHVVAISKTKAWENDTPFQISMIGLFNLNIIWLKLLIPWRLFRLWALLDGIDTPEN
MIRCVDNNYSSLAFWRAWHRSYNKWVVRYIYIPLGGSKNRVLTSLAVFSFVAIWHDIE
LKLLLWGWLIVLFLLPEIFATQIFSHYTDAVWYRHVCAVGAVFNIWVMMIANLFGFCL
GSDGTKKLLSDMFCTVSGFKFVILASVSLFIAVQIMFEIREEEKRHGIYLKC"
gene 356115..358529
/gene="Scy1"
/locus_tag="R008_G11166"
mRNA 356115..358529
/gene="Scy1"
/locus_tag="R008_G11166"
/product="Scy1p"
CDS 356115..358529
/gene="Scy1"
/locus_tag="R008_G11166"
/note="Similar to YGL083W Putative kinase, suppressor of
GTPase mutant, similar to bovine rhodopsin kinase"
/codon_start=1
/product="Scy1p"
/protein_id="EWG86294.1"
/translation="MMFWSSKTGITSKYSFSSSPTFTAEPWSIYTGRPKSSSSSSPSK
VSIFMFDKKQFENYLLHYGIIKSKSGSRDKVLIQEAYEILRNQANNLAKLKHPNILTL
IEPLEEHSKNFMFVTEFVTSSLETVFRETDDEEQNFLQGHVKDNIVVQRGILQLVNAL
DFVHNRASFVHLNIQPRAIFINENSDWKISGLGYLVKIPPGTNTSEYFLPQYDPRVPP
FMHLQLNYTAPEIVFENTLTFKNDYFSLGLLIYFLYTGKDLFRSENSTSEYKLEYNKF
ESKISTMSWDNIFSKVPQKLRHCIPKLINRDIYSRYDNITLILDSEFFQDPLVKTLNF
LDDLPTKNNEEKYVFLEGLVNLLPEFPPALLQKKFLPILLELLSQFCAEKVVSDKCVG
KSLDLIIKIGSTLSQLSFQEKVYPVLLSDANFPVLLKKATICLIDNLDTLKQKVKRSD
FLENILKPLFNYVLHDSESDITVVCQEKLLSQIPLALEVLDFPTVKQFLLPLLSNLFT
KTTSLTVKNTCVTCFQIMIEHKSIDSYTCSETVLPLFKSMKTRDPRILSKLLKLFETV
PLIITDEIVLVDQVLPLMWNYSMAPTLTKSQYSGYTKAINKMSSDIQRHHIAKLDDKV
NDIGEDAFHKVIEPTIMKKEDPETVAAKNIEVAAMQPVKKKTGSSYGETSPQSKSILN
SKPLNPKNALATRGFPTRILNSPPQTPSSRTGSKVMTKGGSNDASSTKVEEEFNEFQS
FSSTGSIRQTSAPSDVWMNSTPSPTPTSASSTNLPPGFSISLQPNKRKDGSSDIPRSN
VYGSLI"
gene 358884..360029
/locus_tag="R008_G11176"
mRNA 358884..360029
/locus_tag="R008_G11176"
/product="hypothetical protein"
CDS 358884..360029
/locus_tag="R008_G11176"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86295.1"
/translation="MDVTFLTKNVQINGTQFKILLQNGQGECALIALANVLLISPAHA
RYAQEISRLVRGKETVTLNELVQTLADMGVQNPNGTDVDKQQLLQILPQLYSGLNINP
EFNGSFEDGVEMSIFRLYNVGIVHGWIIDGDNDPNSYEHVSKYSYMGAQKVLVQSYEI
QKNNAQFENSEQIQSDAPYLKSFLARSATQLTEYGLTHLREILVERSYAVLFRNDHFC
TLYKNNGELFTLVTDPTYRNRKDINWQSLKSVNGSQDSYYTGNFIPTSLERTETTATG
QNESYISNPFSDQNTGHVTSNQVNSGASGVQQIEDDEELARRLQEQEDMRAANNMQNG
YANNGRNHQRERFERPEKNSKKNKFLPFNGSNKEKKRDKLKKNCVIM"
gene 360434..361401
/locus_tag="R008_G11181"
/note="hypothetical protein; nonfunctional due to
frameshift determined by sequencing error"
gene 361698..362090
/gene="Fmp37"
/locus_tag="R008_G11186"
mRNA 361698..362090
/gene="Fmp37"
/locus_tag="R008_G11186"
/product="Fmp37p"
CDS 361698..362090
/gene="Fmp37"
/locus_tag="R008_G11186"
/note="Similar to YGL080W 'Putative protein of unknown
function; highly conserved across species and orthologous
to human gene BRP44L; the authentic, non-tagged protein is
detected in highly purified mitochondria in
high-throughput studies'"
/codon_start=1
/product="Fmp37p"
/protein_id="EWG86296.1"
/translation="MSQPVQRAAARSFLQKYINKETLKYIFTTHFWGPVSNFGIPIAA
IYDLKKDPTLISGPMTFALVTYSGVFMKYALAVSPKNYLLFGCHLINETAQLAQGYRF
LKYTYFTTDEEKKALDKEWKEKEKTGKQ"
gene 362506..363162
/locus_tag="R008_G11196"
mRNA 362506..363162
/locus_tag="R008_G11196"
/product="hypothetical protein"
CDS 362506..363162
/locus_tag="R008_G11196"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86297.1"
/translation="MVTGISEENDDEETFSAVHSSTPSINSQSYAIPITEEMSSSFHD
SISTTSNSSGSFDSDGSNVSDVVEQNEMDNESNVDEDLFLDNDIPQSSNLLPTDAQDP
GPIFDVSRYIFDSLKQSIDSADFSEALSLQTKTSAVINSKSLELKQYIDEMKSRLTQL
QEKFENGEATSKKIKRDLETSRKNIDYLNAALRVDFPIEFNQAREKILERRLNEDHDC
"
gene complement(363349..364920)
/gene="Dbp3"
/locus_tag="R008_G11201"
mRNA complement(363349..364920)
/gene="Dbp3"
/locus_tag="R008_G11201"
/product="Dbp3p"
CDS complement(363349..364920)
/gene="Dbp3"
/locus_tag="R008_G11201"
/note="Similar to YGL078C Putative ATP-dependent RNA
helicase of the DEAD-box family involved in ribosomal
biogenesis"
/codon_start=1
/product="Dbp3p"
/protein_id="EWG86298.1"
/translation="MTKEEIADKKRKVVDEEVIEKKKSKKHKKDKKDKKEKKDKKHKK
HKKEKKGEKEVEVPEKESEKKPEPTSAVASEFYVQSEALTSLPQSDIDEYFKENEIAV
EDPLDLALRPLLSFDYLSLDSSIQAEISKFPKPTPIQAVAWPYLLSGKDVVGVAETGS
GKTFAFGVPAISHLMNDQKKRGIQVLVISPTRELASQIYDNLIVLTDKVGMQCCCVYG
GVPKDEQRIQLKKSQVVVATPGRLLDLLQEGSVDLSQVNYLVLDEADRMLEKGFEEDI
KNIIRETDASKRQTLMFTATWPKEVRELASTFMNNPIKVSIGNTDQLTANKRITQIVE
VVDPRGKERKLLELLKKYHSGPKKNEKVLIFALYKKEAARVERNLKYNGYNVAAIHGD
LSQQQRTQALNEFKSGKSNLLLATDVAARGLDIPNVKTVINLTFPLTVEDYVHRIGRT
GRAGQTGTAHTLFTEQEKHLAGGLVNVLNGANQPVPEDLIKFGTHTKKKEHSAYGSFF
KDVDLTKKPKKITFD"
gene complement(365285..366976)
/gene="Hnm1"
/locus_tag="R008_G11211"
mRNA complement(365285..366976)
/gene="Hnm1"
/locus_tag="R008_G11211"
/product="Hnm1p"
CDS complement(365285..366976)
/gene="Hnm1"
/locus_tag="R008_G11211"
/note="Similar to YGL077C 'Choline/ethanolamine
transporter; involved in the uptake of nitrogen mustard
and the uptake of glycine betaine during hypersaline
stress; co-regulated with phospholipid biosynthetic genes
and negatively regulated by choline and myo-inositol'"
/codon_start=1
/product="Hnm1p"
/protein_id="EWG86299.1"
/translation="MSIRNDNASGGYMQPDQSSNASMHKRDLRVEEEIKPLDDMDSKG
AVAADGEVHLRKSFSLWSILGVGFGLTNSWFGISTSMVAGISSGGPMMIVYGIIIVAL
ISICIGTSLGELSSAYPHAGGQFWWSLKLAPPKYKRFAAYMCGSFAYAGSVFTSASTT
LSVATEVVGMYALTHPEFIPKRWHIFVCFELLHLFLMFFNCYGKSLPIISSSSLYISL
LSFFTITITVLACSHGKFNDAKFVFATFNNETGWKNGGIAFIVGLINPAWSFSCLDCA
THMAFEVEKPERVIPIAIMGTVAIGFVTSFCYVIAMFFSIQDLDAVLSSTTGAPILDI
YNQALGNKSGAIFLGCLILFTSFGCVIACHTWQARLCWSFARDNGLPLSRLWSQVNPH
TGVPLNAHLMSCAWITLIGLLYLASSTAFQSLITGCIAFLLLSYIIPVICLLAKKRNI
AHGPFWLGKFGFFSNIVLLGWTVFSVVFFSFPPVLPVTKDNMNYVCVVIVGYTAYSIL
YWKYKGKKEFHALEESENEQAEYSNNFDTIEDSREFSVAASDVELENEHVPWGKK"
gene complement(367403..369074)
/gene="Rpl7a"
/locus_tag="R008_G11216"
mRNA complement(join(367403..368032,368511..368604,
369064..369074))
/gene="Rpl7a"
/locus_tag="R008_G11216"
/product="Rpl7ap"
CDS complement(join(367403..368032,368511..368604,
369064..369074))
/gene="Rpl7a"
/locus_tag="R008_G11216"
/note="Similar to YGL076C 'Protein component of the large
(60S) ribosomal subunit, nearly identical to Rpl7Bp and
has similarity to E. coli L30 and rat L7 ribosomal
proteins; contains a conserved C-terminal Nucleic acid
Binding Domain (NDB2)'"
/codon_start=1
/product="Rpl7ap"
/protein_id="EWG86300.1"
/translation="MAAEKILTPESQLKKSKAQQKTAEQVAAERAARKAANKEKRAII
LERNAAYQKEYETAERNIIQAKRDAKAAGSYYVEAQHKLVFVVRIKGINKIPPKPRKV
LQLLRLTRINSGTFVKVTKATLELLKLIEPYVAYGYPSYSTIRQLVYKRGFGKINKQR
VPLSDNAIIEANLGKYGILSIDDLIHEIITVGPHFKQANNFLWPFKLSNPSGGWGVPR
KFKHFIQGGSFGNREEFINKLVKSMN"
gene complement(369446..369541)
/locus_tag="R008_G11226"
ncRNA complement(369446..369541)
/ncRNA_class="snoRNA"
/locus_tag="R008_G11226"
/product="SNR39B"
gene complement(369992..371155)
/gene="Mps2"
/locus_tag="R008_G11231"
mRNA complement(369992..371155)
/gene="Mps2"
/locus_tag="R008_G11231"
/product="Mps2p"
CDS complement(369992..371155)
/gene="Mps2"
/locus_tag="R008_G11231"
/note="Similar to YGL075C 'Essential membrane protein
localized at the nuclear envelope and spindle pole body
(SPB), required for insertion of the newly duplicated SPB
into the nuclear envelope; potentially phosphorylated by
Cdc28p'"
/codon_start=1
/product="Mps2p"
/protein_id="EWG86301.1"
/translation="MSNGAFDAIFEYAWGQIDKPISGDFIYGKDLPKLIEIIENIFQK
AQKSGSYELRLPLFSEINKDLFRTFSNTKTFFKIHKEEFDDIFFNLVNHPLREILENA
FIGVDSIPSDFIVSMNLNSPSKFLVENKSKNTEGAGISTPRKKLTESPIKLLSRNNIG
KALEVQVEELKRELTAKQSLLQENERQVSELKIRLETYQEKYASIQQRFSDLQKARQV
EDNQNSSRTSDPGSPLVTGIDQKAILEEFRRRLQRQTDTISFLKDQIRRERGLNCSND
KVSHSKRKHATTDGDGTFKNFISAVPSNIWVKATIRIIVCFALLAGVLPYIRKYVYAH
DTPSQNSRLQLSWWENSGILSKIVWFFEDQTDLETEYRSNANVDDAYSRVFGI"
gene 371821..374322
/gene="Hsf1"
/locus_tag="R008_G11241"
mRNA 371821..374322
/gene="Hsf1"
/locus_tag="R008_G11241"
/product="Hsf1p"
CDS 371821..374322
/gene="Hsf1"
/locus_tag="R008_G11241"
/note="Similar to YGL073W 'Trimeric heat shock
transcription factor, activates multiple genes in response
to stresses that include hyperthermia; recognizes variable
heat shock elements (HSEs) consisting of inverted NGAAN
repeats; posttranslationally regulated'"
/codon_start=1
/product="Hsf1p"
/protein_id="EWG86302.1"
/translation="MNNAANTGTTNESNVSDAPRIEPLPSLNDDDIEKILQPNDIFTT
DRTDASTTSSTAIEDIINPSLDPQSAASPVPSSSFFHDSRKPSTSTHLVRRGTPLGIY
QTNLYGHNSRENTNPNSTLLSSKLLAHPPVPYGQNPNLLQHAVYRAQPSSGTTNAQPR
QTTRRYQSHKSRPAFVNKLWSMLNDDSNTKLIQWAEDGKSFIVTNREEFVHQILPKYF
KHSNFASFVRQLNMYGWHKVQDVKSGSIQSSSDDKWQFENENFIRGREDLLEKIIRQK
GSSNNHSSPSGNGNPANGSNIPLDNAAGINNSNNNISSSNSFFNNGHLLQGKTLRLMN
EANLGDKNDVTAILGELEQIKYNQIAISKDLLRINKDNELLWKENMMARERHRTQQQA
LEKMFRFLTSIVPHLDPKMIMDGLGDPKVNNEKLNSANNIGLNRDNTGTIDELKSNDS
FINDDRNSFTNATTNARNNMSPNNDDNSIDTASTNTTNRKKNIDENIKNNNDIINDII
FNTNLANNLSNYNSNNNAGSPIRPYKQRYLLKNRANSSTSSENPSLTPFDIESNNDRK
ISEIPFDDGEEEETDFRPFTSRDPNNQTSENTFDPNRFTMLSDDDLKKDSHTNDNKHN
ESDLFLDNVHRNIDEQDARLQNLENMVHILSPGYPNKSFNNKTSSTNTNSNMESAVNV
NSPGFNLQDYLTGESNSPNSVHSVPSNGSGSTPLPMPNDNDTEHASTSVNQGENGSGL
TPFLTVDDHTLNDNNTSEGSTRVSPDIKFSATENTKVSDNLPSFNDHSYSTQADTAPE
NAKKRFVEEIPEPAIVEIQDPTEYNDHRLPKRAKK"
gene 375080..377152
/gene="Aft1"
/locus_tag="R008_G11251"
mRNA 375080..377152
/gene="Aft1"
/locus_tag="R008_G11251"
/product="Aft1p"
CDS 375080..377152
/gene="Aft1"
/locus_tag="R008_G11251"
/note="Similar to YGL071W 'Transcription factor involved
in iron utilization and homeostasis; binds the consensus
site PyPuCACCCPu and activates the expression of target
genes in response to changes in iron availability'"
/codon_start=1
/product="Aft1p"
/protein_id="EWG86303.1"
/translation="MEGFNPAHIEHASPINSSDSHSSSFVYALPKSASEYVVNHNEGR
ASASGNPAAVPSPIMTLNLKSTHSLNSDQHVHTSTLPTETIGHIHHVEKLNQNNLIHL
DPVPNFEDKSDIKPWLQKIFYPQGIELVIERSDTFKVVFKCKAAKRGRNARRKRKDKP
KGQDHEDEKSKINDDELEYASPSNATVTNGPQTSPDQTSSIKPKKKRCVSRFNNCPFR
VRATYSLKRKRWSIVVMNNNHSHQLKFNPDSEEYKKFKEKLRKDNDVDAIKKFDELEY
RTLANLPIPTATIPCDCGLTNEIQSFNVVLPTNSNVTSSASSSTVSSISLDSSNASKR
PCLPSVNNTGSINTNNVRKPKSQCKNKDTLLKRTTMQNFLTTKSRLRKTGTPTSSQHS
STAFSGYIDDPFNLNEILPLPASDFKLNTVTNLNEIDFTNIFTKSPHPHSGSTHPRQV
FDQLDDCSSILFSPLTTNTNNEFEGESDDFVHSPYLNSEADFSQILSSAPPVHHDPNE
THQENQDIIDRFANSSQEHNEYILQYLTHFDAANHNNIGVPNNNSHSLNTQHNVSDLG
NPLLRQEALVGSSSTKIFDELKFVQNGPHGSQHPIDFQHVDHRHLSSNEPQVRSHQYG
PQQQPPQQLQYHQNQPHDGHNHEQHQTVQKDMQTHESLEIMGNTLLEEFKDIKMVNGE
LKYVKPED"
gene complement(377527..377895)
/gene="Rpb9"
/locus_tag="R008_G11256"
mRNA complement(377527..377895)
/gene="Rpb9"
/locus_tag="R008_G11256"
/product="Rpb9p"
CDS complement(377527..377895)
/gene="Rpb9"
/locus_tag="R008_G11256"
/note="Similar to YGL070C 'RNA polymerase II subunit
B12.6; contacts DNA; mutations affect transcription start
site selection and fidelity of transcription'"
/codon_start=1
/product="Rpb9p"
/protein_id="EWG86304.1"
/translation="MTTFRFCRDCNNMLYPREDKENNRLLFECRTCSYVEEAGSPLVY
RHELITNIGETAGVVQDIGSDPTLPRSDRECPKCHSRENVFFQSQQRRKDTSMVLFFV
CLSCSHIFTSDQKNKRTQFS"
gene 378155..378739
/gene="Mnp1"
/locus_tag="R008_G11266"
mRNA 378155..378739
/gene="Mnp1"
/locus_tag="R008_G11266"
/product="Mnp1p"
CDS 378155..378739
/gene="Mnp1"
/locus_tag="R008_G11266"
/note="Similar to YGL068W 'Protein associated with the
mitochondrial nucleoid; putative mitochondrial ribosomal
protein with similarity to E. coli L7/L12 ribosomal
protein; required for normal respiratory growth'"
/codon_start=1
/product="Mnp1p"
/protein_id="EWG86305.1"
/translation="MSLRILAKRSSSIWMKTRVTPALISPITITTRFNSTTTTAPSHK
DDVRPVDPKISKIVQDISQLTLLETSSLINELKTVLNIPEISMPMGGFMAGAAGAGAG
NVPSSTGEAGSGAEEEAKPEAKTVFTVKLDSFDTKTKAKVIKEVKGLLGLSLVEAKKF
VEAAPKVLKENVAKDDAEKIKKTLEDLGAKVSLE"
gene 379168..380323
/gene="Npy1"
/locus_tag="R008_G11271"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene 380690..382655
/gene="Sgf73"
/locus_tag="R008_G11276"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(382818..384345)
/gene="Alg2"
/locus_tag="R008_G11281"
/note="nonfunctional due to frameshift determined by
sequencing error"
gene complement(384597..386282)
/gene="Mrh4"
/locus_tag="R008_G11286"
mRNA complement(384597..386282)
/gene="Mrh4"
/locus_tag="R008_G11286"
/product="Mrh4p"
CDS complement(384597..386282)
/gene="Mrh4"
/locus_tag="R008_G11286"
/note="Similar to YGL064C Mitochondrial ATP-dependent RNA
helicase of the DEAD-box family, plays an essential role
in mitochondrial function"
/codon_start=1
/product="Mrh4p"
/protein_id="EWG86306.1"
/translation="MSLFFKPVISPQWSFPVLLKIGVRSYAGGPRTKHKGNSPLASVP
TGSSNKNRKQKAKGKKGNKKNDPDQAFNFGEYGGLKKDVEMNMDSTNKLIQKISNFDQ
LLILPPVRDAVKEIISKESLKLQDSRKKTSENIIPSPIQTVAIKRISKNLMDPKLQIH
AIAAETGSGKTMAYLIPLIDYLKRQELETPELWETLRKNVLIRSIILVPTHELVDQVY
ETVSKTKTLLGLNSFKWDKATSYRDLLENIKNRIDILVTTPGKLLNLFSIRMITRPDK
VLSKVGFVVLDEADTLLDRSWLEETHSAIKRIPNINHLIFCSATIPQEFNKTMQRLFP
TVVPIMTPRLHKLPFALDFKVINSALSPFKGSKIKALAQTLYAISNDDTEPGFEKRCI
IFVNEKKNVPEIVNLLNKKFGHNAIGLTGEDTFEERSEKIMPFLSPPRPLSEVVAQST
SPPTSLKKFEIPDSNIVIGKLKNTNSNGTAPSNKSLHVLVTTDLMARGLNFKGVRNVV
LYDVPKTSIDLIHRVGRTARMKQGGRVFMLTDSKTKSWAKALPKIIKKHQRLS"
gene 386728..387840
/gene="Pus2"
/locus_tag="R008_G11291"
mRNA 386728..387840
/gene="Pus2"
/locus_tag="R008_G11291"
/product="Pus2p"
CDS 386728..387840
/gene="Pus2"
/locus_tag="R008_G11291"
/note="Similar to YGL063W 'Mitochondrial
tRNA:pseudouridine synthase; acts at positions 27 and 28,
but not at position 72; efficiently and rapidly targeted
to mitochondria, specifically dedicated to mitochondrial
tRNA modification'"
/codon_start=1
/product="Pus2p"
/protein_id="EWG86307.1"
/translation="MLLGYCGSGYYGMQYNPPHKTIEGEILTKLFDVGAISEENSLAP
KKNSFMAAARTDKGVHAMLNLLSLKITLREDTVAKLNAALPPEIRVWGIQPVNKKFNA
RSACDSRWYQYLIPEFILIGPPRSSLLHRNVGGCYREDGSQEVWDTFLEQTRGRFSGD
ELCRLQDTAQKLSESDPLVQDYVGLLSGTLSGYCLPPSKLDAFEAAMQEYVGTHNFHN
FTTGKLWGDPSAQRHIKKVVVSQASPGWICVRIHGQSFMLHQIRRMVALAVLAARCQL
PPNIVRNYFNAGPRKYIPRAPAQGLLLEGPVFDGYNTKLRNLLYCEIRPDDITLERMC
RFRERQICTAIAHEETQRHVFCHFVRQMNRLATPLI"
gene 388270..391806
/gene="Pyc1"
/locus_tag="R008_G11301"
mRNA 388270..391806
/gene="Pyc1"
/locus_tag="R008_G11301"
/product="Pyc1p"
CDS 388270..391806
/gene="Pyc1"
/locus_tag="R008_G11301"
/note="Similar to YGL062W 'Pyruvate carboxylase isoform,
cytoplasmic enzyme that converts pyruvate to oxaloacetate;
highly similar to isoform Pyc2p but differentially
regulated; mutations in the human homolog are associated
with lactic acidosis'"
/codon_start=1
/product="Pyc1p"
/protein_id="EWG86308.1"
/translation="MSQRKFAGLRDNFNLLGEKNKILVANRGEIPIRIFRTAHELSMQ
TVAIYSHEDRLSTHKQKADEAYVIGEVGQYTPVGAYLAIDEIISIAQKHQVDFIHPGY
GFLSENSEFADKVAKAGITWIGPPAEVIDSVGDKVSARNLAAKANVPTVPGTPGPIET
VEEALDFVNEYGYPVIIKAAFGGGGRGMRVVREGDDVADAFQRATSEARTAFGNGTCF
VERFLDKPKHIEVQLLADNHGNVVHLFERDCSVQRRHQKVVEVAPAKTLPREVRDAIL
TDAVKLAKECGYRNAGTAEFLVDNQDRHYFIEINPRIQVEHTITEEITGIDIVAAQIQ
IAAGASLPQLGLFQDKITTRGFAIQCRITTEDPAKNFQPDTGRIEVYRSAGGNGVRLD
GGNAYAGTIISPHYDSMLVKCSCSGSTYEIVRRKMIRALIEFRIRGVKTNIPFLLTLL
THPVFIEGTYWTTFIDDTPQLFQMVSSQNRAQKLLHYLADVAVNGSSIKGQIGLPKLK
SNPSVPHLHDAQGNVINVTKSAPPSGWRQVLLEKGPAEFARQVRQFNGTLLMDTTWRD
AHQSLLATRVRTHDLATIAPTTAHALAGAFALECWGGATFDVAMRFLHEDPWERLRKL
RSLVPNIPFQMLLRGANGVAYSSLPDNAIDHFVKQAKDNGVDIFRVFDALNDLEQLKV
GVDAVKKAGGVVEATVCFSGDMLQPGKKYNLDYYLEIAEKIVQMGTHILGIKDMAGTM
KPAAAKLLIGSLRAKYPDLPIHVHTHDSAGTAVASMTACALAGADVVDVAINSMSGLT
SQPSINALLASLEGNIDTGINVEHVRELDAYWAEMRLLYSCFEADLKGPDPEVYQHEI
PGGQLTNLLFQAQQLGLGEQWAETKRAYREANYLLGDIVKVTPTSKVVGDLAQFMVSN
KLTSDDVRRLANSLDFPDSVMDFFEGLIGQPYGGFPEPFRSDVLRNKRRKLTCRPGLE
LEPFDLEKIREDLQNRFGDVDECDVASYNMYPRVYEDFQKMRETYGDLSVLPTRSFLS
PLETDEEIEVVIEQGKTLIIKLQAVGDLNKKTGEREVYFDLNGEMRKIRVADRSQKVE
TVTKSKADMHDPLHIGAPMAGVIVEVKVHKGSLIKKGQPVAVLSAMKMEMIISSPSDG
QVKEVFVSDGENVDSSDLLVLLEDQVPVETKA"
gene complement(392024..392767)
/gene="Duo1"
/locus_tag="R008_G11311"
mRNA complement(392024..392767)
/gene="Duo1"
/locus_tag="R008_G11311"
/product="Duo1p"
CDS complement(392024..392767)
/gene="Duo1"
/locus_tag="R008_G11311"
/note="Similar to YGL061C 'Essential subunit of the Dam1
complex (aka DASH complex), couples kinetochores to the
force produced by MT depolymerization thereby aiding in
chromosome segregation; is transferred to the kinetochore
prior to mitosis'"
/codon_start=1
/product="Duo1p"
/protein_id="EWG86309.1"
/translation="MSEQSQLDDSTIDKLIPQIFNEMRSNLNNTTNKFPKSTGGGASD
NISANSNSIRSFNSITTQSLLKESESLDKITAMIKNVTAALKNNLPVYVNQVHEVCKS
TNSILDSWINIHSQAGYIHKLMSDQTYLKLINDRLHNENVNTNDEDGSTLHNVIALKK
KEILDLRQKLENRKGEKDAAPAKPPNQGLNPRYGVQSGRRPVSSAGISNNGRVRKTHV
PASKRPSGIPRVTNRWTKPTASSSRKMFR"
gene 393121..395046
/gene="Ybp2"
/locus_tag="R008_G11316"
mRNA 393121..395046
/gene="Ybp2"
/locus_tag="R008_G11316"
/product="Ybp2p"
CDS 393121..395046
/gene="Ybp2"
/locus_tag="R008_G11316"
/note="Similar to YGL060W 'Central kinetochore associated
protein that mediates mitotic progression; interacts with
several central kinetochore proteins and the centromeric
histone Cse4p; role in resistance to oxidative stress;
similarity to Ybp1p and Slk19p'"
/codon_start=1
/product="Ybp2p"
/protein_id="EWG86310.1"
/translation="MYNEQVNSGKSIKEKERYLDALLKILKDNPVTLKEIGWDLPKGL
LQFFSRKNINVNIHLVFSPLVSSVMECFNELAINGNPKECLLTACELVSTLHIVLTET
GDSDEENEDLNDSNRNDASNITDELSVITPEIGQYMANNTVEFIPNLKIYVLFEFMSL
LLKRVDTLYPSKFLAMVTSAIIKYVTTNVQAMDDPHFILRIVYNFCTNYSPAQPSASL
TDGISTNDLEKIHDDESALQKKLLANLSVFVISNCLKNHPGNIDKIYFKTLMHKKTDE
NEIDASVLQTCHQYYEYVTSLDVHMKELLEKCLVESRSIYNSLLMNPAASTPEFKEEI
NQLVYEVSYAYQIKKLADEKNLELDQYGVVILSAIHYSKNGTHLLPQIDIQSAIYLYL
RCTTASLFSEIYENKFLESSVRYWLWVSTTETSTEKIKCALQELPGHITTAFLQMLLM
KTCNESNNDTKLTEITLLRRLLYLMPESTSFTFIFETLLHCPYITAKIAVLDILRDMM
IRSPEAANRDETVGLIEQQNPGNTANSVPIMPTLPPRPYITINEDRMASIHSIALICF
SAAKQKKRTQGDLLLVLTYMKFFVSLRNKWDLGLLTLINKEISESFQGEGEPELAFIN
ISNNTLGEYIEEMNIRS"
gene 395279..396754
/locus_tag="R008_G11321"
mRNA 395279..396754
/locus_tag="R008_G11321"
/product="hypothetical protein"
CDS 395279..396754
/locus_tag="R008_G11321"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86311.1"
/translation="MSKYQINCIRYRHFLRTSNISQIPDFTKYCIGPVNEELAPYIME
TMKAYPSNSEYINPQHYYHNRTVLVENYLKRSPNPVSLTQLAQYYDDSTKLTRTKIIN
SGKFVKEELVIRIAHKLNQLQQLPFNVVNNFHFVQVYESYYNIFESFRKYPTIRTLED
ASQFADFIKNMLEGFNTLNLPHLIMGALECTILDLYPREKMDQLLSDLLRARISRRLI
VEEHVSITANYTSGKEENTLVLGDIFQECSAKKYLLEASEESQKFIQDMYFKDIPMPE
FIIEGDTQLSFYFLPTHLKYLLGEILRNTYEATMKHYIRKGLEKPEPIIVTVVSNDES
YLFRISDKAGGVLHDDENLWSFGKSKERAQESLNNFHKLPGLQTVSIYDEVHSHTKYN
SKLKSLQSITLKPYMHTSLEPMSYPSIINGHIKYETPLIELLKRSFRYKLGIGLAMCK
VYAEYWNGDLSLHSMPGYGTDVVLKLGNLMKHTKKLQLDKV"
gene 397042..397557
/gene="Rad6"
/locus_tag="R008_G11326"
mRNA 397042..397557
/gene="Rad6"
/locus_tag="R008_G11326"
/product="Rad6p"
CDS 397042..397557
/gene="Rad6"
/locus_tag="R008_G11326"
/note="Similar to YGL058W Ubiquitin-conjugating enzyme
(E2), involved in postreplication repair (as a heterodimer
with Rad18p), DSBR and checkpoint control (as a
heterodimer with Bre1p), ubiquitin-mediated N-end rule
protein degradation (as a heterodimer with Ubr1p"
/codon_start=1
/product="Rad6p"
/protein_id="EWG86312.1"
/translation="MSTPARRRLMRDFKRMKEDAPPGVSASPLPDNVMVWNAMIIGPA
DTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDILQNRWTPTYDV
ASILTSIQSLFNDPNPASPANVEAATLFKDHKSQYVKRVKETVEKSWEDDMDDMDDDD
DDDDDDDDEAD"
gene complement(398018..398881)
/locus_tag="R008_G11331"
mRNA complement(398018..398881)
/locus_tag="R008_G11331"
/product="hypothetical protein"
CDS complement(398018..398881)
/locus_tag="R008_G11331"
/codon_start=1
/product="hypothetical protein"
/protein_id="EWG86313.1"
/translation="MVLSNVKIFRLKSHRAFRIGPMIKAVAGNLLVKRFYQPKLERIP
PASLLLKQKIRLAQNGSTTSTENPISFSQTMSEIFSVLQPSAPDLDEDETSGLKRDHL
LTERLNNGELGVIMNKFFNPSSTHNNQLIDTNILLQNFPKLSGNDLDLLDFAINEKMR
GNWNDLKQDFIQLWYYKSFGFLGPRTQFVLTNSSPSLRSQFLKLPFIEYNWFLLQNNK
NANILPADVQNVVKVFHLDDKRFTWKSIDPFSKAIISFVVFVSIYVWLDESAKQKTKE
LPAQKSTVISE"
gene complement(399083..400666)
/gene="Sds23"
/locus_tag="R008_G11336"
mRNA complement(399083..400666)
/gene="Sds23"
/locus_tag="R008_G11336"
/product="Sds23p"
CDS complement(399083..400666)
/gene="Sds23"
/locus_tag="R008_G11336"
/note="Similar to YGL056C 'One of two S. cerevisiae
homologs (Sds23p and Sds24p) of the S. pombe Sds23
protein, which is implicated in APC/cyclosome regulation;
involved in cell separation during budding'"
/codon_start=1
/product="Sds23p"
/protein_id="EWG86314.1"
/translation="MPQNTRHTSIVEMLSTPPQLPNSTDLNSLSEQTDKNTEANKSDT
ESLHKSISKSSSSSSLSTLDNTEYSNNNGNSLSTLNSQNLLSVHRQEWQHTPLSNLVE
QNKLIFIRGSISVEEAFNTLVKHQLTSLPVENFPGDMNCLTFDYNDLNAYLLLVLNRI
KVSNDKITSDCQNGKSVPVGEIVKLTPKNPFYKLPETENLSTVIGILGSGVHRVAITN
VEMTQIKGILSQRRLIKYLWENARSFPNLKPLLDSSLEELNIGVLNAARDKPTFKQSR
VISIQGDEHLIMALHKMYVERISSIAVVDPQGNLIGNISVTDVKHVTRTSQYPLLHNT
CRHFVSVILNLRGLETGKDSFPIFHVYPTSSLARTFAKLVATKSHRLWIVQPNDNQPT
ASSEKSSSPSPSTPPVTTLPSLASSYHSNTQSSRMANSPVLKSSDTSNNKINVNINLS
GPSPSQPQSPSTAMPPPQSPSNCPASPTPAHFEKEYRTGKLIGVVSLTDILSVLARKQ
THHKEIDPQMARKQRGHIG"
gene 401684..403216
/gene="Ole1"
/locus_tag="R008_G11346"
mRNA 401684..403216
/gene="Ole1"
/locus_tag="R008_G11346"
/product="Ole1p"
CDS 401684..403216
/gene="Ole1"
/locus_tag="R008_G11346"
/note="Similar to YGL055W Delta(9) fatty acid desaturase,
required for monounsaturated fatty acid synthesis and for
normal distribution of mitochondria"
/codon_start=1
/product="Ole1p"
/protein_id="EWG86315.1"
/translation="MPTSGTTIELIDDQFPKDDSASSGIVDEVDLTEANILATGLNKK
APRIVNGFGSLMGSKEMVSVEFDKKGNEKKSNLDRLLEKDNQEKEEAKTKIHISEQPW
TLNNWHQHLNWLNMVLVCGMPMIGWYFALSGKVPLHLNVFLFSVFYYAVGGVSITAGY
HRLWSHRSYSAHWPLRLFYAIFGSASVEGSAKWWGHSHRIHHRYTDTLRDPYDARRGL
WYSHMGWMLLKPNPKYKARADITDMTDDWTIRFQHRHYILLMLLTAFVIPTLICGYFF
NDYMGGLIYAGFIRVFVIQQATFCINSMAHYIGTQPFDDRRTPRDNWITAIVTFGEGY
HNFHHEFPTDYRNAIKWYQYDPTKVIIYLTSLVGLAYDLKKFSQNAIEEALIQQEQKK
INKKKAKINWGPVLTDLPMWDKQTFLAKSKENKGLVIISGIVHDVSGYISEHPGGETL
IKTALGKDATKAFSGGVYRHSNAAQNVLADMRVAVIKESKNSAIRMASKRGEIYETGK
FF"
CONTIG join(APIP01000093.1:1..66952,gap(5186),APIP01000094.1:1..15729,
gap(230),APIP01000095.1:1..25038,gap(5272),
APIP01000096.1:1..139297,gap(480),APIP01000097.1:1..19517,gap(252),
APIP01000098.1:1..6354,gap(14),APIP01000099.1:1..44892,gap(52),
APIP01000100.1:1..73978)
//