LOCUS KF318042 8618 bp DNA circular SYN 09-APR-2014 DEFINITION Functional analysis vector pFA-HAH2, complete sequence. ACCESSION KF318042 VERSION KF318042.1 KEYWORDS . SOURCE Functional analysis vector pFA-HAH2 ORGANISM Functional analysis vector pFA-HAH2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 8618) AUTHORS Sharma,S., Alfatah,M., Bari,V.K., Rawal,Y., Paul,S. and Ganesan,K. TITLE Sphingolipid Biosynthetic Pathway Genes FEN1 and SUR4 Modulate Amphotericin B Resistance JOURNAL Antimicrob. Agents Chemother. 58 (4), 2409-2414 (2014) PUBMED 24395234 REFERENCE 2 (bases 1 to 8618) AUTHORS Paul,S., Rawal,Y., Alfatah,M., Bari,V.K., Sharma,S., Choudhury,S., Malik,S. and Ganesan,K. TITLE Single-Transformation Gene Function Test by Conditional Expression of Both Alleles of Candida albicans Genes JOURNAL Unpublished REFERENCE 3 (bases 1 to 8618) AUTHORS Paul,S., Rawal,Y., Alfatah,M., Bari,V.K., Sharma,S., Choudhury,S., Malik,S. and Ganesan,K. TITLE Direct Submission JOURNAL Submitted (03-JUL-2013) Institute of Microbial Technology, Sector 39-A, Chandigarh, UT 160036, India REMARK Single-Transformation Gene Function Test by Conditional Expression of Both Alleles of Candida albicans Genes COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8618 /organism="Functional analysis vector pFA-HAH2" /mol_type="other DNA" /db_xref="taxon:1411083" misc_feature 22..55 /note="LoxLE" gene 60..928 /gene="HIS1" /note="5' region; derived from Candida albicans HIS1" repeat_region 575..928 /gene="HIS1" /rpt_type=direct gene 934..2884 /gene="ARG4" /note="derived from Candida albicans ARG4" CDS 1391..2797 /gene="ARG4" /note="argininosuccinate lyase" /codon_start=1 /transl_table=11 /product="arg4" /protein_id="AGY46360.1" /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDAD LTGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRL GEIIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDV LMPGYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGI DREFLAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFG FIKLADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQE DKEPLFDALTTVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFR ETHHISGECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGG TAKSAVLKQLENLKSILS" gene 2889..3738 /gene="HIS1" /note="3' region; derived from Candida albicans HIS1" repeat_region 2889..3242 /gene="HIS1" /rpt_type=direct misc_feature 3739..3772 /note="LoxRE" gene complement(4606..5625) /gene="URA3" CDS complement(4606..5409) /gene="URA3" /note="derived from S. cerevisiae URA3; orotidine-5'-phosphate decarboxylase" /codon_start=1 /transl_table=11 /product="Ura3" /protein_id="AGY46361.1" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" regulatory complement(5410..5625) /regulatory_class="promoter" /gene="URA3" /note="derived from S. cerevisiae URA3" misc_feature 5895..6398 /note="CEN/ARS; S. cerevisiae CEN6 centromere fused to an autonomously replicating sequence" gene 6540..7400 /gene="bla" CDS 6540..7400 /gene="bla" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AGY46362.1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 2553 a 1736 c 1759 g 2570 t ORIGIN 1 cttcgtacgc tgcaggtcga ctaccgttcg tataatgtat gctatacgaa gttatggatc 61 ctggaggatg aggagacaga agttagtagt aacaatttgg ggacagaaga ggatacagtg 121 acatcagagg aagaagacag cgaaggtata gtcaaaaagc aataggacag caagacaatt 181 tatttattta atagtttttt ataaatgtaa aatctatcac aacatgtgta cttaattttc 241 tgtggtaggg gaggccattt cgttgaaacg tgtcgcgagc ggttgtgaga attgactcat 301 tttgattatt acgcaaccat tgaaattttt tatttttttt tggtgaagat ttttcccaca 361 caacttcttc ttttacttaa ccaccaacta ccgataatgg atttagtcaa tcatttacca 421 gaccgtttgt tatttgctgt tcctaaaaag ggcagattat acgaaaaatg ctgtaactta 481 ttgagtggtg ccgatataca gtttagaaga tctaatagat tagatatagc actttctaca 541 aacttgccaa ttgcattaat cttcttgcct gcagctgata tcccagtttt cgttggagaa 601 ggtaattgtg acttgggtat aactgggtta gaccaaatca aagaagctga acaattcgac 661 aacatcgagg acttgttgga tttgaaattt ggttcatgca aattgcagat ccaagttcca 721 gcagatggcg agtacgaaaa gccagaacag cttgttggaa agaaaattgt gtcttcattt 781 acaaaattga gtaccgacta tttcaaacaa ttgtcagaca aacctactaa tatcagatat 841 gtcggtggtt ccgttgaggc ttcttgtgcc ttgggtgttg ctgatgctat tgtcgatttg 901 gttgaaagtg gtgaaactat gaaagcagga tccccccttt agtaagattt ttcaagagta 961 gtctcaaata aacagaatga aggattgaag gttttattca ataaataatt ggacagaaag 1021 ttattgcaca gttttaacaa caacaatttc aaaatttcaa tttatgacat attgaccgac 1081 ataattcagt catttctaat attcaaataa caagacacct tgtataaatg aatccacaag 1141 cgtatatgaa caaccaaatt ttatgactaa ctgtgggtcg tgtacttttg acgattgagt 1201 catttttacc aattaggtaa ttgaatttta ctaatttgtc attaaccgcg ctggccatcc 1261 caacaacaac aacaacaatc aatttaaaaa cttgacaagg caaaaaaaaa aaatttcatc 1321 tttgaaaaat ttttcttcct tttcttcttc tgttctttac ataacagcta tcaagaataa 1381 tcaattaata atgtcacaac aacaagataa acaacctagt gaaaataaat tatggggtgg 1441 tcgtttcacc ggtgccactg atccattgat ggatttgtat aatgcttcat taccttatga 1501 taaagttatg tatgatgctg atttaactgg tactaaagta tacacacaag gattaaacaa 1561 attaggatta attaccactg aagaattgaa tttgattcat caaggattag aacaaatcag 1621 acaagaatgg catgataata aattcattat taaggctggt gatgaggata ttcatactgc 1681 taatgaacgt cgattaggag aaatcattgg gaaaaacatt tctggtaaag ttcacactgg 1741 tagatcaaga aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg aatcattact 1801 taatttaagt aaaattttac atcaattcat cacggcaatt cttgaacgag cacataaaga 1861 aatcgatgta ttaatgcctg gttatactca tttacaaaaa gctcaaccta ttagatgggc 1921 ccattggtta agttcatatg caacttattt caccgaagat tataaacgtt tacaagaaat 1981 tataactcgt gttaaccaat ctccattagg atctggtgct ttagcaggtc atccttatgg 2041 tattgatcgt gaatttttag ctaaaggatt aggatttgat ggtgtcattg ggaattcttt 2101 aactgcagtt tcagatcgtg attttgttgt tgaaagttta ttttggagta cattatttat 2161 gaatcatatt tctcgattct cagaagatct aattatttat tctagtggtg aatttgggtt 2221 cataaaatta gctgatgctt attctactgg ttcttcattg atgccacaaa agaaaaaccc 2281 tgactcatta gaattattac gtgggaaaag tggtagagta tttggtcagt tatctggatt 2341 tttaatgtcg atcaaatcta ttccatctac ttataataaa gatatgcaag aggataaaga 2401 accattattt gatgcattga ctacagtaga acattcaatt ttaattgcta ctggtgtgat 2461 ttctacttta ctgattgata aacaaaatat ggaaaaagct ttaactatgg atatgttggc 2521 tactgattta gctgattatt tagttagaaa aggagttcca tttagagaaa ctcatcatat 2581 atctggtgaa tgtgttagaa aagctgaaga agaaaaatta tctggaattg atcaattatc 2641 ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa gacgttatgg aaacttttga 2701 ttttgaagct agtgtggaaa gaagagatgc tcttggtggt actgctaaaa gtgccgtttt 2761 aaaacaatta gaaaatttaa aatcaatctt aagttaattt ataaatagtc atataataat 2821 cacagtattg tttaacttac ataaaatata atcaattaac acagagatac cttgtactac 2881 aatagtttct gatatcccag ttttcgttgg agaaggtaat tgtgacttgg gtataactgg 2941 gttagaccaa atcaaagaag ctgaacaatt cgacaacatc gaggacttgt tggatttgaa 3001 atttggttca tgcaaattgc agatccaagt tccagcagat ggcgagtacg aaaagccaga 3061 acagcttgtt ggaaagaaaa ttgtgtcttc atttacaaaa ttgagtaccg actatttcaa 3121 acaattgtca gacaaaccta ctaatatcag atatgtcggt ggttccgttg aggcttcttg 3181 tgccttgggt gttgctgatg ctattgtcga tttggttgaa agtggtgaaa ctatgaaagc 3241 agctgggtta aaggccatag aaaccatatt ggagacttcg gctcatttga tttcgtcaaa 3301 aaaaagtaaa ttcccagaaa tggtcaatat aatcgtccaa agacttcaag gtgttttagc 3361 tgcccaggaa tatgtcttgt gtaactacaa tgccccaaaa tccatccaag caaaatgttt 3421 aaccattact ccaggcagaa gggccgctac tgtctctact ttggacaaac acagcgacga 3481 cgaagaggac tgggttgcca tctcatccat ggttaataga aaggaaatcg gtaatgtaat 3541 ggacgaattg aagaaagctg gtgcaaccga tatattggta ctcgagatat caaattgtag 3601 agtttaaaag aagtgatagt ttctcataaa tattatacat atatacttat agatctactt 3661 caatttgact caatctgtct tataccatct ttatctacca ccttgatgta cacaccttta 3721 aagtcaatag gcattctcat aacttcgtat aatgtatgct atacgaacgg tagtttaaac 3781 gagctcgaat tcatcgatga tatcagatcc actagtggta cccaattcgc cctatagtga 3841 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 3901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 3961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga 4021 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 4081 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 4141 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 4201 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 4261 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 4321 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 4381 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 4441 cgcgaatttt aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg 4501 catctgtgcg gtatttcaca ccgcataggg taataactga tataattaaa ttgaagctct 4561 aatttgtgag tttagtatac atgcatttac ttataataca gttttttagt tttgctggcc 4621 gcatcttctc aaatatgctt cccagcctgc ttttctgtaa cgttcaccct ctaccttagc 4681 atcccttccc tttgcaaata gtcctcttcc aacaataata atgtcagatc ctgtagagac 4741 cacatcatcc acggttctat actgttgacc caatgcgtct cccttgtcat ctaaacccac 4801 accgggtgtc ataatcaacc aatcgtaacc ttcatctctt ccacccatgt ctctttgagc 4861 aataaagccg ataacaaaat ctttgtcgct cttcgcaatg tcaacagtac ccttagtata 4921 ttctccagta gatagggagc ccttgcatga caattctgct aacatcaaaa ggcctctagg 4981 ttcctttgtt acttcttctg ccgcctgctt caaaccgcta acaatacctg ggcccaccac 5041 accgtgtgca ttcgtaatgt ctgcccattc tgctattctg tatacacccg cagagtactg 5101 caatttgact gtattaccaa tgtcagcaaa ttttctgtct tcgaagagta aaaaattgta 5161 cttggcggat aatgccttta gcggcttaac tgtgccctcc atggaaaaat cagtcaagat 5221 atccacatgt gtttttagta aacaaatttt gggacctaat gcttcaacta actccagtaa 5281 ttccttggtg gtacgaacat ccaatgaagc acacaagttt gtttgctttt cgtgcatgat 5341 attaaatagc ttggcagcaa caggactagg atgagtagca gcacgttcct tatatgtagc 5401 tttcgacatg atttatcttc gtttcctgca ggtttttgtt ctgtgcagtt gggttaagaa 5461 tactgggcaa tttcatgttt cttcaacact acatatgcgt atatatacca atctaagtct 5521 gtgctccttc cttcgttctt ccttctgttc ggagattacc gaatcaaaaa aatttcaaag 5581 aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa ttgaattgaa aagctgtggt 5641 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc 5701 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca 5761 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg 5821 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat 5881 ggtttcttag gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg 5941 gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag 6001 aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt 6061 aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt 6121 aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt 6181 ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca 6241 agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa 6301 aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt 6361 aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt 6421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 6481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 6541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 6601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 6661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 6721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 6781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 6841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 6901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 6961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 7021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 7081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 7141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 7201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 7261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 7321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 7381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 7441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 7501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 7561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 7621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 7681 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 7741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 7801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 7861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 7921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 7981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 8041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 8101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 8161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 8221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 8281 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 8341 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 8401 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc 8461 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcctatg ttgtgtggaa 8521 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg 8581 caattaaccc tcactaaagg gaacaaaagc tggagctc //