LOCUS KE124922 540790 bp DNA linear CON 06-JUN-2013
DEFINITION Ancylostoma ceylanicum unplaced genomic scaffold
A_ceylanicum-1.0_Cont148, whole genome shotgun sequence.
ACCESSION KE124922 ASSL01000000
VERSION KE124922.1
DBLINK BioProject: PRJNA72583
KEYWORDS WGS; HIGH_QUALITY_DRAFT.
SOURCE Ancylostoma ceylanicum
ORGANISM Ancylostoma ceylanicum
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae;
Ancylostomatinae; Ancylostoma.
REFERENCE 1 (bases 1 to 540790)
AUTHORS Mitreva,M.
TITLE Draft genome of the parasitic nematode Anyclostoma ceylanicum
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 540790)
AUTHORS Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C.,
Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K.
TITLE Direct Submission
JOURNAL Submitted (14-MAY-2013) The Genome Institute, Washington University
School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA
COMMENT Ancylostoma ceylanicum is a parasite of humans and carnivores in
Asia. The parasite was adapted to the Syrian golden hamster
(Mesocricetus auratus) in 1972 by Ray and Bhopale. The strain
(Indian) was distributed worldwide from the lab of Dr. Jerzy Behnke
in the 1980's. The sequenced strain was obtained by Dr. John M.
Hawdon (jhawdon@gwu.edu) from Dr. Ricardo Fujiwara at the Federal
University of Minas Gerais, Brazil. The strain was maintained in
Dr. Hawdon's lab in dogs and hamsters since 2007. Worm isolation
and extraction of nucleic acids was performed by Dr. Verena
Gelmedin and others in the Hawdon lab, or the Genome Institute
production team. Voucher specimens are on deposit in the U.S.
National Parasite Collection (accession number 102954). For the
original isolation and adaptation to hamsters see Ray, D.K.,
Bhopale, K.K., 1972. Complete development of Ancylostoma ceylanicum
(Looss, 1911) in golden hamsters, Mesocricetus auratus. Experientia
28, 359-361
This assembly consists of fragments, 3kb and 8kb insert whole
genome shotgun libraries. The sequences were generating on the
Roch/454 platform and assembled using Newbler. To improve
scaffolding, inhouse tools CIGA (Cdna tool for Improving Genome
Assembly) and Pygap (Gap closure tool) were used to map 454 cDNA
reads using blat to the genomic assembly to link genomic contigs
based on cDNA evidence. Only joins confirmed by additional
independent data typing were accepted and close gaps followed by
the Pyramid assembler and Illumina paired reads to closing gaps and
extending contigs
The repeat library was generated using Repeatmodeler (A.F.A. Smit,
R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA
genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic
Acids Res.) and transfer RNA's were identified with tRNAscan-SE
(Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as
microRNAs, were identified by sequence homology search of the Rfam
database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.).
Repeats and predicted RNA's were then masked using RepeatMasker (A.
Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding
genes were predicted using a combination of ab initio programs Snap
(Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V.
2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008
Bioinformatics) and the annotation pipeline tool Maker (M. Yandell
et. al., 2007 Genomc Research) which aligns mRNA, EST and protein
information from same species or cross-species to aid in gene
structure determination and modifications. A consensus gene set
from the above prediction algorithms was generated, using a
logical, hierarchical approach developed at the Genome institute.
Gene product naming was determined by BER
(http://ber.sourceforge.net).
Our goal is to explore this WGS draft sequence of A. ceylanicum to
better define proteins involved in nematode parasitism that impact
health and disease and are relevant to both host-parasite
relationships and basic biological processes.
For information regarding this assembly or project, or any other
GSC genome project, please visit our Genome Groups web page
(http://genome.wustl.edu/genome_group_index.cgi) and email the
designated contact person. For specific questions regarding the A.
ceylanicum genome project contact Makedonka Mitreva
(mmitreva@genome.wustl.edu) at Washington University School of
Medicine. The National Human Genome Research Institute (NHGRI) of
the National Institutes of Health (NIH) provided funds for this
project.
##Genome-Assembly-Data-START##
Current Finishing Status :: High-Quality Draft
Assembly Method :: Newbler v.
MapAsmResearch-04/19/2010-patch-
08/17/2010
Assembly Name :: A_ceylanicum1.3.ec.cg.pg
Genome Coverage :: 26.10x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..540790
/organism="Ancylostoma ceylanicum"
/mol_type="genomic DNA"
/submitter_seqid="A_ceylanicum-1.0_Cont148"
/specimen_voucher="USDA:USNPC:102954"
/db_xref="taxon:53326"
/chromosome="Unknown"
gene 1762..6208
/locus_tag="ANCCEY_05972"
mRNA join(1762..1776,2364..2422,5150..5198,5938..6035,
6091..6208)
/locus_tag="ANCCEY_05972"
/product="hypothetical protein"
CDS join(1762..1776,2364..2422,5150..5198,5938..6035,
6091..6208)
/locus_tag="ANCCEY_05972"
/inference="protein motif:HMMPfam:IPR002123"
/note="KEGG: cel:ZK40.1 1.2e-27 acl-9;
ACyLtransferase-like family member (acl-9); K13513
lysocardiolipin and lysophospholipid acyltransferase"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74910.1"
/db_xref="InterPro:IPR002123"
/translation="MFPAKLVVILTDAKLVMYESDSSVRMGAGEPPVLAAGQPFWSLM
SYVFGAKVRVRGDMIDHSKPAVIIMNHRTRLDWLYFWNALYKMDPWLCTSEKITLKGV
LKYLPGAGLV"
assembly_gap 6527..6739
/estimated_length=213
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(7118..12707)
/locus_tag="ANCCEY_05973"
mRNA complement(join(7118..7232,7290..7385,7459..7584,
7675..7767,7858..7917,7978..8118,8219..8278,8536..8636,
8700..8777,8846..8916,8984..9097,9155..9368,9432..9614,
9671..9808,9880..10002,10073..10115,10173..10312,
10368..10431,10491..10618,11851..11962,12532..12707))
/locus_tag="ANCCEY_05973"
/product="DNA replication licensing factor Mcm7-A family
protein"
CDS complement(join(7118..7232,7290..7385,7459..7584,
7675..7767,7858..7917,7978..8118,8219..8278,8536..8636,
8700..8777,8846..8916,8984..9097,9155..9368,9432..9614,
9671..9808,9880..10002,10073..10115,10173..10312,
10368..10431,10491..10618,11851..11962,12532..12707))
/locus_tag="ANCCEY_05973"
/inference="protein motif:HMMPfam:IPR001208"
/note="KEGG: tgo:TGME49_037220 6.8e-107 DNA replication
licensing factor, putative K02210"
/codon_start=1
/product="DNA replication licensing factor Mcm7-A family
protein"
/protein_id="EPB74911.1"
/db_xref="InterPro:IPR001208"
/translation="MDLSQPTAYEAEIMHYLVVEATFSVVIQHTIQSRSIRPERPIEE
DERVASHSPSFKMMMHFFKMAFNKEGMDYDKHKALIREFIDTYYGVDESGAKVFYYRE
DIQRIAEREQVALYVNLDDINRFDESLAVLIEGNARRFHQIFNEVIDEMVQEILGDRQ
PPIRDALDAFIFQRVYMDDQSKIADGYLGGTIQEARKKYPPQLLRRFEVIFKNRDAMK
PLAANCVGKLVTVSGIVIRATEVKPIVEVMTYACDTCGAEVYQPVNGPSFMPAVNCPS
KDCVESKANGRLHMQVRGSKFGKFQEIKIQETSDQVPVGSIPRTLTVNIYGEATRQCA
PGDHVRISGVLIPLMRTGFRQGGGGLVAETFLEAHFVENIRSSVDEKDTDDDLTEEEV
ELLAQDNLYDMLAYSIAPEIYGLTDVKKSLLLALVGGVDRNASGMKIRGCLNVLLMGD
PGVAKSQLLSYVNRLAPRSQYTTGRGSSGVGLTAAVVKDPVTGEMTLEGGALVLADRG
ICCIDEFDKMMESDRTSIHEVMEQQTISIAKAGIMTTLNARVAIVAAANPAFGRYNPK
RTIEQNIDLPAALLSRFDLLWLIQDKPDRDGDKRLASHITYVHMNAKQPEVDGMKPID
MRLIRRLIEVAQRKQPVVGDTLRERLVEMYVDMRRVARESDDSTFTSPRLLLSVIRMS
TALARLRLSNVVLPDDIEEAIRLMQASKDSLRPEMLQQEIRQSPIDRAFAVLRELNSS
AGDAVIALQTAVEACARKGISEEALRDAITVHQSNGVIMVDSQQRIRFVMN"
assembly_gap 13218..13317
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 27551..27939
/estimated_length=389
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 30305..30648
/estimated_length=344
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 33682..34084
/estimated_length=403
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 35836..37087
/estimated_length=1252
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 39095..39788
/estimated_length=694
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 50325..51879
/estimated_length=1555
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 53091..53190
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 64705..64776
/locus_tag="ANCCEY_16026"
tRNA 64705..64776
/locus_tag="ANCCEY_16026"
/product="tRNA-Ala"
assembly_gap 66865..66964
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 69665..69998
/estimated_length=334
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 71062..71598
/estimated_length=537
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 72334..72968
/estimated_length=635
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 73747..74493
/estimated_length=747
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 77862..77961
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 80457..86253
/locus_tag="ANCCEY_05974"
mRNA join(80457..80477,85388..85480,86164..86253)
/locus_tag="ANCCEY_05974"
/product="hypothetical protein"
CDS join(80457..80477,85388..85480,86164..86253)
/locus_tag="ANCCEY_05974"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74912.1"
/translation="MCMPLWRSDKITEVDLFLENCSSLYNLILDALHDLSSKSTLSVA
LSELPFFFGNRGHPFNAAHQQLI"
assembly_gap 81128..81302
/estimated_length=175
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 81999..82575
/estimated_length=577
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 84019..84635
/estimated_length=617
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 87336..87998
/estimated_length=663
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 90965..91192
/estimated_length=228
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 92937..93036
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 93937..94036
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 95396..116233
/locus_tag="ANCCEY_05975"
mRNA join(95396..95407,106299..106391,106879..106960,
109550..109674,110187..110212,116227..116233)
/locus_tag="ANCCEY_05975"
/product="hypothetical protein"
CDS join(95396..95407,106299..106391,106879..106960,
109550..109674,110187..110212,116227..116233)
/locus_tag="ANCCEY_05975"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74913.1"
/translation="MLRKALSCQHLDEMANSVAAAGRLLVAKRPETETTVALRINTIN
SAMEQLRLRRCTSDSDTCSSRSQPSSELRGWIHSQEKRLLELEQRLKEGVGLTKLLSD
QQYSLGAKSLSQ"
assembly_gap 97987..98086
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 112098..112197
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 117386..133122
/locus_tag="ANCCEY_05976"
mRNA join(117386..117553,121119..121183,126372..126513,
127399..127430,129417..129478,131566..131585,
133018..133122)
/locus_tag="ANCCEY_05976"
/product="hypothetical protein"
CDS join(117386..117553,121119..121183,126372..126513,
127399..127430,129417..129478,131566..131585,
133018..133122)
/locus_tag="ANCCEY_05976"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74914.1"
/translation="MLCEDKKEHREEQAVGALREETGSHRIVDSISTFGSAVSLWRWA
LTQIPGQFRLGMILQLEIQSDGQALINRLYQQFREEHDDRTAEVLAKRKLGFDAIRKR
WHNLYLNSLCLQCRIEEAINRFQEFVLFCFVCIRHNNPERIHENLKVSEPNKLRIYYI
HHKLFIDEKGYGAVSSINIHLLHVILPAILLAGSRQI"
assembly_gap 120009..120292
/estimated_length=284
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 122479..122578
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 131041..131539
/estimated_length=499
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 133616..134976
/estimated_length=1361
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 138567..160447
/locus_tag="ANCCEY_05977"
mRNA join(138567..138622,143439..143570,148681..148768,
149071..149167,155765..155955,156694..156761,
158068..158143,158203..158344,158404..158564,
160376..160447)
/locus_tag="ANCCEY_05977"
/product="hypothetical protein"
CDS join(138567..138622,143439..143570,148681..148768,
149071..149167,155765..155955,156694..156761,
158068..158143,158203..158344,158404..158564,
160376..160447)
/locus_tag="ANCCEY_05977"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74915.1"
/translation="MPECKKKHHSDTTNSMERSGTDMSVFSEKSPGVGRKWDSGVLDI
GYSSGENSLLDVITDGDTSITNIELMMRKRAEAKTSAGKLHDLTAFEEPTHEGFTDVD
RDPLTDSMHVNYDALNGNYPEYADNKNILRVKIRHFADEVMALLEDGTNLAPVHISDS
FNTKWREIGGHAEKTRRRRTRDDDEEAKHSCDASSEDSSDWESRDDRLMNRSFNDFGG
GGGSLPGARIDPKSSSFLLDSSPLDASFCSTKSELTQGQCRTRKRLRVRRLPRSMSDG
EQLIVRSGAFTPQIRRSPPATPLARSTRLLQKLDRDLLANVESDTAPEQSDTQVYEWD
EYNITPFKTMDRYFFGSWPKTVRSKG"
assembly_gap 138698..138797
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 141287..141417
/estimated_length=131
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 142605..143081
/estimated_length=477
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 143745..144236
/estimated_length=492
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 149396..150275
/estimated_length=880
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 150866..150965
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 151517..152188
/estimated_length=672
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 154101..154569
/estimated_length=469
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 156208..156409
/estimated_length=202
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 161979..172894
/locus_tag="ANCCEY_05978"
mRNA join(161979..162056,166506..166616,166681..166773,
168517..168672,168728..168888,171006..171179,
171238..171336,171397..171528,172594..172894)
/locus_tag="ANCCEY_05978"
/product="hypothetical protein"
CDS join(161979..162056,166506..166616,166681..166773,
168517..168672,168728..168888,171006..171179,
171238..171336,171397..171528,172594..172894)
/locus_tag="ANCCEY_05978"
/note="KEGG: chu:CHU_0040 2.8e-05 DNA repair ATPase
K03546"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74916.1"
/translation="MSDTGDILDMLDLQLLVDNCHNLAINPPAKDDSMNEPPSPLSPT
VRDDLLEVDEDFSEHFDAWDSLRRLVAESRAHLRVVEKSINSGEVDAAQLENVQMIAR
ANLRQLDTVLRMSGGKNEDVAGLREQWKLLSEQAASPIPQLLSKVECFANSLRDLHET
SSASSLNAMTDIKSKEDVRLALEVVSQIQAKLSKERDELRELLASPLMVSELSELSSE
FNSISTGYDDAVNRINSLVSSLKKLDQVWTDWSEQLKGIRDQMNRIEGSLKADKFDQL
AISEEMELCQERMNSLETMCNYLTSSLQSVQGTESAATIPDFRSEITLYGNAMEQLKS
KFQEIYRIPTPPGPTTVPNEPQQSPRRRVKTQSTPTQTIVTPRLNQSLGSRVYRAVAE
STAIKLSILLSILAGLAVLFYTGILGATFGPHLTYVNGPPPV"
assembly_gap 164229..164868
/estimated_length=640
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 167326..168071
/estimated_length=746
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 169914..170013
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 174297..174970
/estimated_length=674
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 176641..176793
/estimated_length=153
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 179078..179177
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 184057..184245
/estimated_length=189
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 185263..185362
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 193139..196662
/estimated_length=3524
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 196906..199002
/locus_tag="ANCCEY_05979"
mRNA join(196906..196980,197043..197123,198862..199002)
/locus_tag="ANCCEY_05979"
/product="hypothetical protein"
CDS join(196906..196980,197043..197123,198862..199002)
/locus_tag="ANCCEY_05979"
/note="KEGG: rno:66015 3.6e-05 Adamts4; ADAM
metallopeptidase with thrombospondin type 1 motif, 4
K07764"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74917.1"
/translation="MKALLISVALFHALDAFSLSFFSSQPTTPYLHPLSPPETNPASA
RAKRQAYQVYVDGESSVSLDKTGQTESGPWGQWTPEECSRTCGGGVQIEKRQCS"
gene 201288..214071
/locus_tag="ANCCEY_05980"
mRNA join(201288..201340,201370..201525,202704..202855,
204046..204219,204278..204420,205008..205149,
205206..205300,205356..205475,205835..205917,
206858..206926,207324..207456,207543..207635,
207689..207810,207926..208058,208138..208253,
208584..208711,209137..209204,209263..209418,
209500..209599,209722..209841,209921..210086,
210244..210351,210393..210555,210623..210779,
210963..210999,211150..211321,211387..211513,
211611..211765,211837..211941,211995..212064,
212298..212384,212443..212589,212724..212840,
212903..213100,213176..213264,213321..213423,
213902..214071)
/locus_tag="ANCCEY_05980"
/product="Kunitz/Bovine pancreatic trypsin inhibitor
domain protein"
CDS join(201288..201340,201370..201525,202704..202855,
204046..204219,204278..204420,205008..205149,
205206..205300,205356..205475,205835..205917,
206858..206926,207324..207456,207543..207635,
207689..207810,207926..208058,208138..208253,
208584..208711,209137..209204,209263..209418,
209500..209599,209722..209841,209921..210086,
210244..210351,210393..210555,210623..210779,
210963..210999,211150..211321,211387..211513,
211611..211765,211837..211941,211995..212064,
212298..212384,212443..212589,212724..212840,
212903..213100,213176..213264,213321..213423,
213902..214071)
/locus_tag="ANCCEY_05980"
/inference="protein motif:HMMPfam:IPR000884"
/inference="protein motif:HMMPfam:IPR002223"
/inference="protein motif:HMMPfam:IPR010294"
/note="KEGG: phu:Phum_PHUM547410 3.2e-142 papilin,
putative"
/codon_start=1
/product="Kunitz/Bovine pancreatic trypsin inhibitor
domain protein"
/protein_id="EPB74918.1"
/db_xref="InterPro:IPR000884"
/db_xref="InterPro:IPR002223"
/db_xref="InterPro:IPR010294"
/translation="MLAMSHKCLHAESYVIYKGDCTGPSVRYVSCNLDPCPDGTDFRA
EQCAKHNDDALDGHYHKWQPYKGKNKCELLCKPEGGNFYYKWDEKVVDGTKCDAKGDD
ICVDGVCLPVGCDGKLGSALKVDKCGVCDGDGSKCKTIEGTFDERNLSPGYHDVMRLP
AGATAIKIEEARPSSNNLALKNSTDHFFLNGNSMIQVERDVELNGVYFEYDDSKPERI
IAKGPLKEDVTVSVLFRKGNRDAAIKYEFSIPLVEDVDYMYKPGEWSSCSVTCGKGVQ
TRTPYCIDTKTQRRVNDALCDNANYTKPEFEKACETVDCEAEWFEGDWEPCSQTCGDQ
GEQYRVVYCHQVYANGRRVTVEDGNCTSERPSVRQVCNRCGGGSQERVAVCLNYDKKP
VPEWFSCPEWQAGPWSACSEKCGDAFQYRSVTCRSEKEGEEGKLLPADACDPEKTVES
QRSCNLGPCEGLKFFTSEWKLCSKCNDTEETREVTCKDNMGRAYPLEKCLTEEEKEIP
SDTRACATQQPCIYEWTASQWSKCSTECGHGHKTRRVICAIHEEGDITIVDEGLCSGE
KPEDKTNCTNEEKCTGTWYSGPWSPCSAECGGGSQERVAVCLNYDKKPVPEWCDEATM
PVLTQECNVDPCPTCFDSEFGCCPDNTTFATGDFNQGCSNCTLSEFGCCADNYTEATG
KNGMGCEEFVESPLNLEEGKEEEGSGDQDAKPTECQVTNEQGEMATVDCAVANATVTD
VDDLFGNGTDTNATMHCSKSEFGCCPDWFTAAEGPNNAGCPVFVLGVCNETEYGCCHD
DVTLARGPNLEGCGEPTCAGSLYGCCKDRKTIAFGPHYAGCERSSFPCELSSFGCCSD
GETAALGPNGTGCGENCLTTKSDLDMAAAQTAKVSRRVIIMKGAVACMPNTVAVPMAR
HRLKEPASMDVQIAARKVNSDAVPMAKRRLEDHTKKAAHVSTHDMDAVLMGKQPLLDQ
EMMDAMTADMLTKGLIRENEATHRYGCCPDGESKAIGPDYAGCPSTTLAPFLLGGTVA
PSKISSCALPQDQGTVCSSGYKLVWYYDTTEGRCSQFWYGGCDGNDNRFATKEQCETI
CVEPPGIGRCYLPKVEGPLRCDVPQARYWYDYNTKQCAAFWWRGCHGNANNFASWEEC
STFCKDVGPFEIPTTQPPPTQPQPFIQPEIPEIGHEVVAHPVVDEVPAPRVDPRPRQP
MPTIEEVCRSTQDSGPCQDYSDQYYYDAYKGTCQTFIYGGCGGNLNRFRTEEECMQRC
GFLNPTVAAVHAGHQAGPVHQGHEHQHQQQPHPHEQEMVRPPPPVVPHQQPAHAVQPH
SHVKSRQVCHLPLDVGKCQGSFDSWYYEMATGSCVEFKYSGCSGNANRFASRSDCEAT
CVRQHDASAGDASEGATSICDESKDTGPCTNFVTKWYYNKADGTCNRFHYGGCEGTGN
RFDNEQSCKAACGNHQDACTLPKVQGPCSGKHEYFFFNTISQQCEKFVYGGCLGNTNR
FSTQEECQSRCPRK"
assembly_gap 202214..202313
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 203319..203634
/estimated_length=316
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 206957..207056
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 216235..230264
/locus_tag="ANCCEY_05981"
mRNA join(216235..216254,216433..216568,216736..216900,
217402..217505,217590..217668,219287..219450,
219629..219745,219831..219984,220057..220143,
222866..222986,224430..224470,225442..225523,
225898..226013,226169..226302,226666..226792,
228082..228174,228872..228967,229041..229196,
229249..229368,229981..230055,230121..230264)
/locus_tag="ANCCEY_05981"
/product="Protein-tyrosine phosphatase"
CDS join(216235..216254,216433..216568,216736..216900,
217402..217505,217590..217668,219287..219450,
219629..219745,219831..219984,220057..220143,
222866..222986,224430..224470,225442..225523,
225898..226013,226169..226302,226666..226792,
228082..228174,228872..228967,229041..229196,
229249..229368,229981..230055,230121..230264)
/locus_tag="ANCCEY_05981"
/inference="protein motif:HMMPfam:IPR000242"
/inference="protein motif:HMMPfam:IPR002223"
/inference="protein motif:HMMPfam:IPR013098"
/note="KEGG: cel:F47B3.7 6.5e-70 hypothetical protein;
K01104 protein-tyrosine phosphatase"
/codon_start=1
/product="Protein-tyrosine phosphatase"
/protein_id="EPB74919.1"
/db_xref="InterPro:IPR000242"
/db_xref="InterPro:IPR002223"
/db_xref="InterPro:IPR013098"
/translation="MDAGECRAYETAYYYDYFNGVCKEFVYGGCGGNANRFETADDCA
TRCARRQQGRRELSRSFYDDDDLQPVVTNNDNDQEAGDDKQEETFDDDINDDVLRGID
YDEKKVLAIQRGSLSPYTLPELCGLPEEHGSCYDDILRWRFDPEKKSCTSFMYGGCDM
NANHFTSEEDCERACGSWRSTAVCSLPPEIGNCEASVSKWYYDKAKRTCTIMYWTGCG
GNGNSKMPRSSGPCRDAISMWYFDSSDETCKQFTYSGCRGNENRFATKEQCDRKCIQK
RDDDDFSVIIGEDEVTKRTVSVQLKVNSDKGFTVGEKIELTCDSQGLQPIVWRKNGHL
LQFTRRVQEHDKFTKVKIARAIMADSGEYQCAAGPDGILSNAIAVSVTRVEKVTRIIN
RRIKGSLRAPVEFEKNYGAKLWLASSHSCRKDDKHGGAGDREGDGVEREAQVVVDRSF
KVARTSQDAKHMVQHWVNRALDMGVPRLMEEFKYSLSKWQPEGMTVNEFLANRDKNRY
LDVPCQDYRRVVVRWPGLPNDYIHANYVANPTVDNRFICAQGPLDNTQLAFWAMVIQE
KSECVIMLCNTIECGKFKCSQYWPHEKGESMTFGEGPGRILVTNLDVRPLSEIDNFVR
VCRLRLDFYENGRETSTMIRHFQWENWPDRGVPPTKLTATNILSEVRGTTRPIVVHCS
AGIGRTGTIVAIAYVQEKMQHGQNCMDMDALTKELRTQRPFSIQNEYQYIYVHRVLLA
YFLEKYRDRFAHILAGEGEAKYAKWREEYKIATGCD"
assembly_gap 218297..219246
/estimated_length=950
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(230320..237293)
/locus_tag="ANCCEY_05982"
mRNA complement(join(230320..230490,231125..231250,
232439..232506,232560..232698,234686..234833,
234900..235079,235204..235325,235527..235565,
235936..236055,237228..237293))
/locus_tag="ANCCEY_05982"
/product="transporter, major facilitator family protein"
CDS complement(join(230320..230490,231125..231250,
232439..232506,232560..232698,234686..234833,
234900..235079,235204..235325,235527..235565,
235936..236055,237228..237293))
/locus_tag="ANCCEY_05982"
/inference="protein motif:HMMPfam:IPR005828"
/note="KEGG: isc:IscW_ISCW006472 1.2e-13 sugar
transporter, putative"
/codon_start=1
/product="transporter, major facilitator family protein"
/protein_id="EPB74920.1"
/db_xref="InterPro:IPR005828"
/translation="MSSNSDEAEQQKLHEDFVEEKQFDLLCEDGHLVKNSISIQMLGV
LIGAMISGQLSDRYGRKTKLIQGDNYSNNSEVLIASLLGVSVFSLGTALAFTFVQFTV
LRAVVGIFTGGLSAVQGVFLIENIPKHHRMWINTIVTWSPNFIIYPVIAYWCHDWRKL
SMFTAAIGVVATVNLCCLHESPRWLIHHGRIDRARLVLAHIRKLNRKTCEKEAEEIEA
MLMHEQSIIRWGVNIMVGVGDYFCKRVGRKLINFVAITFIMGALAIICGLYLQDLQIK
EAWVIRYCTIGVTAMTSQLYIAKFMITNELFPTAVRNIAVSALSVASRLGTIIAPQLF
YLADVLPVLPYMVLLVLSFIDCISFHFFLPETKGTNLENHLPPKDQKIFYRKQSVLEE
"
assembly_gap 231339..231525
/estimated_length=187
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 233710..234357
/estimated_length=648
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(242359..243420)
/locus_tag="ANCCEY_05983"
mRNA complement(join(242359..242502,242644..242718,
242976..243140,243346..243420))
/locus_tag="ANCCEY_05983"
/product="Protein-tyrosine phosphatase"
CDS complement(join(242359..242502,242644..242718,
242976..243140,243346..243420))
/locus_tag="ANCCEY_05983"
/inference="protein motif:HMMPfam:IPR000242"
/note="KEGG: cel:F47B3.7 3.6e-14 hypothetical protein;
K01104 protein-tyrosine phosphatase"
/codon_start=1
/product="Protein-tyrosine phosphatase"
/protein_id="EPB74921.1"
/db_xref="InterPro:IPR000242"
/translation="MQHFWAMVIQEKSDYILMLCNTVECKIQVHPMSDEDSFIRVSKI
RLDYKDGGNEASRTVHHYHWENWPDRGVPPTKLTAIEDCQAMSELLKEIRTQRPCSIQ
NAYQYLYVHRVLLAYFLEKYKRRFQHCLDNGGEEKYQKWCADYKRITGCD"
gene complement(249155..249661)
/locus_tag="ANCCEY_05984"
mRNA complement(249155..249661)
/locus_tag="ANCCEY_05984"
/product="hypothetical protein"
CDS complement(249155..249661)
/locus_tag="ANCCEY_05984"
/note="KEGG: bca:BCE_5423 3.8e-08 NADH dehydrogenase
subunit C K00332"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74922.1"
/translation="MAKLQEELRRAKSAEGKGSGEAVKNKDTSAMKRGKFAPKYSTLL
LKSGSGSKEKTKTTSKEDTNPASKEDVVAPAKRKSKEKSAEAFVAPAQGKSKEKSAEA
FVAPAQGKSKEKSAEAFVAPAQGKSKEKSAEDIVAPAKGKSKEKSAEEFVVPAKGKSK
EKSAEASV"
assembly_gap 250512..250753
/estimated_length=242
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 252282..253526
/estimated_length=1245
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 260499..260598
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 269472..270159
/estimated_length=688
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 275614..276367
/estimated_length=754
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(276876..277537)
/locus_tag="ANCCEY_05985"
mRNA complement(join(276876..277006,277091..277200,
277256..277363,277419..277537))
/locus_tag="ANCCEY_05985"
/product="Myb-like DNA-binding domain protein"
CDS complement(join(276876..277006,277091..277200,
277256..277363,277419..277537))
/locus_tag="ANCCEY_05985"
/codon_start=1
/product="Myb-like DNA-binding domain protein"
/protein_id="EPB74923.1"
/translation="MQDIAHDFAEMAQYVEVNGNDSEPVQVVQSPKTKCHEETKAAMR
ELTKVRSRAVRLECTLKAQRAEGLMSGLDSYRYLVSKEDRRDEEGSSRRDRVRGSHTW
TEEERIIAFHCLLRYGRDFDAVAEVLGTKTSDKVKSFYTDMKADIDKVSDCYS"
gene 278799..281342
/locus_tag="ANCCEY_05986"
mRNA join(278799..278944,280124..280310,280361..280477,
280611..280786,280846..281017,281097..281342)
/locus_tag="ANCCEY_05986"
/product="Protein-tyrosine phosphatase"
CDS join(278799..278944,280124..280310,280361..280477,
280611..280786,280846..281017,281097..281342)
/locus_tag="ANCCEY_05986"
/inference="protein motif:HMMPfam:IPR000242"
/note="KEGG: dmo:Dmoj_GI14758 1.8e-30 GI14758 gene product
from transcript GI14758-RA; K01104 protein-tyrosine
phosphatase"
/codon_start=1
/product="Protein-tyrosine phosphatase"
/protein_id="EPB74924.1"
/db_xref="InterPro:IPR000242"
/translation="MTDVHTPASNPIPSEESEKTSTTGGSEVQRECSSCHVSIVSNLD
ANDSRHATEDHDYIHANWVDGYREPKKFILTQAPLGQTINQFWNMIWQEKCSVVVSMI
QMFDVNGAEWTISYIPKRTGEERNAGDIKLIHCGTRCIRRTYDATLIKAIRNGSERRL
LHLCYFAWGHRGTPRNPTEVLNFIADLNCNRELLVKEAVAAGWLKANENSPIVVHCIT
GVARSATLVALDICLKKLDDTANRPCGPLADVEDVVMRIRTQRAMAVQKAEMYLFLHL
AVFEYAVRQRYIAEETYSEVDIGGFFYEPRHEEQEGLRTESLSSSNTSDSKDKDASTK
RDQQDEKPSTQKK"
gene complement(282053..284011)
/locus_tag="ANCCEY_05987"
mRNA complement(join(282053..282199,282313..282474,
282804..282928,283052..283157,283224..283331,
283835..284011))
/locus_tag="ANCCEY_05987"
/product="Myb-like DNA-binding domain protein"
CDS complement(join(282053..282199,282313..282474,
282804..282928,283052..283157,283224..283331,
283835..284011))
/locus_tag="ANCCEY_05987"
/inference="protein motif:HMMPfam:IPR014778"
/note="KEGG: mpe:MYPE6470 8.5e-06 parC; DNA topoisomerase
IV subunit A; K02621 topoisomerase IV subunit A"
/codon_start=1
/product="Myb-like DNA-binding domain protein"
/protein_id="EPB74925.1"
/db_xref="InterPro:IPR014778"
/translation="MSSDPKEEEVDESILDEPVEGANLAADEEIDESILDHEMEIDAP
SNPPSELHSDAHSEGSPPHSSHEIKEETSNDAQHTDTDEKPKQVSGESTPKKTPTKRA
RRSRARRNETDDEIDDDEKDEDMESDHKAMLSSADRKINVGDDFQARVEESQKDDQML
PQLSEEEREREYALYILQKSNFDFDVAREKVKKRRLITEEWCEDDRTLFKQAFHMFGK
RFDKIRQTMPHRSMASIIQFYYNTKKDTDYKSLFDSRIADDSDEEEGFVSKIIFLY"
assembly_gap 291920..293138
/estimated_length=1219
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(294792..295184)
/locus_tag="ANCCEY_05988"
mRNA complement(294792..295184)
/locus_tag="ANCCEY_05988"
/product="core histone H2A/H2B/H3/H4"
CDS complement(294792..295184)
/locus_tag="ANCCEY_05988"
/inference="protein motif:HMMPfam:IPR007125"
/note="KEGG: tgo:TGME49_100200 9.1e-32 histone H2A K11251"
/codon_start=1
/product="core histone H2A/H2B/H3/H4"
/protein_id="EPB74926.1"
/db_xref="InterPro:IPR007125"
/translation="MSGRGKGGKSKSGTKAKSRSARAGLQFPVSRVHRKLRRGNYATR
IGAGAPVYLAAVLEYLSAEVLELAGNAARDNKKTRINPRHLQLAIRNDEELNKLLCRV
TISQGGVLPHIHAVLLPKKTASEKEEKQ"
gene 295350..295813
/locus_tag="ANCCEY_05989"
mRNA join(295350..295702,295771..295813)
/locus_tag="ANCCEY_05989"
/product="hypothetical protein"
CDS join(295350..295702,295771..295813)
/locus_tag="ANCCEY_05989"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74927.1"
/translation="MPPKPSAKGAKKSGRAPKTPKASRSGERKKRCHRKESHSGCIRC
AVELVRRYDVKYDVEHGFVRYRRPRAHRCCGVATCAQQQAVEISSREVQTSAHLILSG
ELAKLAVSEGTKTVTKATIMVEYSNKLHD"
assembly_gap 296401..296538
/estimated_length=138
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 299469..300910
/estimated_length=1442
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 302437..303579
/estimated_length=1143
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 304318..306168
/estimated_length=1851
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 310245..310709
/estimated_length=465
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 313711..313810
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 314287..314508
/locus_tag="ANCCEY_05990"
mRNA join(314287..314377,314438..314508)
/locus_tag="ANCCEY_05990"
/product="hypothetical protein"
CDS join(314287..314377,314438..314508)
/locus_tag="ANCCEY_05990"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74928.1"
/translation="MFERFLRIFIFIFYLFDTVFASYEKNNNGKDIKKIRPAALNRPV
QMDQFVMPS"
assembly_gap 316525..317301
/estimated_length=777
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 318240..318671
/estimated_length=432
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 326015..326114
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 328607..329364
/estimated_length=758
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 331219..332404
/locus_tag="ANCCEY_05991"
mRNA join(331219..331245,331695..331991,332204..332404)
/locus_tag="ANCCEY_05991"
/product="hypothetical protein"
CDS join(331219..331245,331695..331991,332204..332404)
/locus_tag="ANCCEY_05991"
/note="KEGG: ret:RHE_CH03555 1.1e-11 ypch01255;
5'-nucleotidase protein K01081"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74929.1"
/translation="MRSFYYLFTPVHVHVAPAPVVAPAPVVEAVPVVAPAPVVAPAPI
VEAAPVMAAPVMAHAHVVAPAPVVGPPPIVAPAPVVAPAPIVAPAPVVAPAPVVAPAP
VVAPAPKLVTPPTPQWSREMTTMATTALAYGTPSHSATSQTTTTMTTTTRMVTTTVAM
ASMALGTRSSPPRK"
gene complement(334442..337348)
/locus_tag="ANCCEY_05992"
mRNA complement(join(334442..334677,334855..335133,
335375..335406,337332..337348))
/locus_tag="ANCCEY_05992"
/product="hypothetical protein"
CDS complement(join(334442..334677,334855..335133,
335375..335406,337332..337348))
/locus_tag="ANCCEY_05992"
/note="KEGG: kpe:KPK_3473 9.0e-08 rne; ribonuclease E
K08300"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74930.1"
/translation="MVTQRRADASYPHHEHVAPAPVVEAVPVVAPAPVVAPAPIVEAA
PVMAAPVMAHAHVVAPAPVVAPPPIVAPAPVVAPAPVVEAAPIVAPAPVVAPAPVFPY
AMVSFSFQTGHTSDTAMVSGDDHHGHHGFGLWDSFPFGHFSDDHHHDHHYPYGHYLGG
YGFYGPWHEKLAAKKAAKSMKTFEKKN"
assembly_gap 345287..345487
/estimated_length=201
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 347534..347866
/estimated_length=333
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 356661..356760
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 357922..358334
/estimated_length=413
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 383887..384349
/estimated_length=463
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 387247..396342
/locus_tag="ANCCEY_05993"
mRNA join(387247..387335,387396..387561,390593..390721,
390794..390916,392656..392856,394122..394208,
394511..394683,395025..395169,396139..396342)
/locus_tag="ANCCEY_05993"
/product="K+ channel tetramerisation domain protein"
CDS join(387247..387335,387396..387561,390593..390721,
390794..390916,392656..392856,394122..394208,
394511..394683,395025..395169,396139..396342)
/locus_tag="ANCCEY_05993"
/inference="protein motif:HMMPfam:IPR003131"
/inference="protein motif:HMMPfam:IPR005821"
/note="KEGG: isc:IscW_ISCW006705 1.4e-129 voltage-gated
channel, putative K05320"
/codon_start=1
/product="K+ channel tetramerisation domain protein"
/protein_id="EPB74931.1"
/db_xref="InterPro:IPR003131"
/db_xref="InterPro:IPR005821"
/translation="MDSEYRIILNVGGVRHETYKHTLKKIPATRLSRLTTNLANYDPV
LNEYFFDRHPGVFSQILNYYRTGKLHYPLDVCGPLFEEELKYWGLDSNECEPCCWMTL
SGTRDTQEVLKTLDELDIEVDNADGPGLYQRFGWEDDYHNDSLSSWQKLKPKIWRLFD
EPSSSRAAKIIAIISVFFLITAILVFCLKTHPGLRISEISGVGNLSREARAANHYRYR
PASLTVDKTNSKAHPGFMMVETLCNIWFTIEILVRFTCCPSRMEYLKAPVNLIDIVAT
VTFYMDVLFNTFGASADLEFFSIIRIMRLFKLTHHNSGLKILMHTFRASAKELMLLVF
FLVLGVVVFASLVYYAERVEPNPNNEFQSIPIGLWWAVIVSNFAMFYSHTQARSKMPK
KRRGVLSVDQVKQQPHRANGPRRHGEHTPLVTPNINNHASPPHTMI"
assembly_gap 391839..391938
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 395851..395965
/locus_tag="ANCCEY_16249"
ncRNA 395851..395965
/ncRNA_class="other"
/locus_tag="ANCCEY_16249"
/product="Potassium channel RNA editing signal"
/inference="nucleotide motif:Rfam:RF00485"
/note="Rfam score 61.00"
gene complement(398045..400109)
/locus_tag="ANCCEY_05994"
mRNA complement(join(398045..398367,398980..399118,
399209..399301,399406..399468,399533..399623,
399899..400000,400057..400109))
/locus_tag="ANCCEY_05994"
/product="pinin/SDK/MemA/ protein region"
CDS complement(join(398045..398367,398980..399118,
399209..399301,399406..399468,399533..399623,
399899..400000,400057..400109))
/locus_tag="ANCCEY_05994"
/inference="protein motif:HMMPfam:IPR006786"
/note="KEGG: edi:EDI_156940 3.3e-09 trichohyalin"
/codon_start=1
/product="pinin/SDK/MemA/ protein region"
/protein_id="EPB74932.1"
/db_xref="InterPro:IPR006786"
/translation="MDYDDMDDEDDRDIRPKRTLASTVVMPSIETKSREERIEEMNKT
EKKEVTTRNRRMFSNLLMGTLTRFQKDEKKIQNVEKIQAEKQKEVEKRLEDKKREDRE
KIMAERQKLFDERREQEKELRALQRKKALIQYAEAKKEHYKLLRNFIQTQTKPTLFFA
PAKHSMRSLELLKASEKAIDGLMELRQKELERELENSVDDDHQEHNTSGANEAEAGNS
KEANTPRRRRDRSYSHSGDEDDHEKSPEPADGKEKSAEPEGVHENNDEDDAMGEEERI
QPEEEPMDKDD"
assembly_gap 400883..401322
/estimated_length=440
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 402183..402306
/estimated_length=124
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(402359..405954)
/locus_tag="ANCCEY_05995"
mRNA complement(join(402359..402473,402578..402687,
402739..402842,402911..403007,403675..403804,
403859..403920,404143..404296,404352..404410,
404648..404817,405033..405116,405795..405954))
/locus_tag="ANCCEY_05995"
/product="molybdopterin binding domain protein"
CDS complement(join(402359..402473,402578..402687,
402739..402842,402911..403007,403675..403804,
403859..403920,404143..404296,404352..404410,
404648..404817,405033..405116,405795..405954))
/locus_tag="ANCCEY_05995"
/inference="protein motif:HMMPfam:IPR001453"
/inference="protein motif:HMMPfam:IPR005110"
/inference="protein motif:HMMPfam:IPR005111"
/note="KEGG: apb:SAR116_2510 1.7e-37 molybdopterin
biosynthesis protein MoeA K03750"
/codon_start=1
/product="molybdopterin binding domain protein"
/protein_id="EPB74933.1"
/db_xref="InterPro:IPR001453"
/db_xref="InterPro:IPR005110"
/db_xref="InterPro:IPR005111"
/translation="MAENDKVQNISSVVTRPRQSTWPALPMNEALAFVESVDVPRCVK
YIPVAAVRVGQVLAERVVAKEDVPEVRTSIKDGYAVLASDPPGERRVIGSSTAGAPFL
GTISAGECVRVSTGAFVPDGADAVAMVENTRLVKHDGIEELIISVDTTVKPGQDIRMP
GSDIRKGDLLLDSGCVLGSAEIGILAGSGKRSVLMYRKPKVCVLSTGNELIALFGSLG
FKAIDAGIAADRRECLVEAIRVSFQYAHVLVTSGGVSMGEKDLLKDVLMKDFGFEIHF
GRVWMKPGLPTTFATGHMFGDKKFVFALPGNPVSSWVTAQLFAVPLLRKAAGHTRLFQ
QEIKVQLAEDINLDSRPEYRRAWLQHGGAIPTAITTGNQISSRLMSLSGANVLLKVPG
KSAECTRLPAGEIVDALWLGAM"
assembly_gap 406760..407392
/estimated_length=633
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 411394..412426
/estimated_length=1033
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 413959..414388
/estimated_length=430
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 420764..421853
/estimated_length=1090
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 422629..423214
/estimated_length=586
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 434398..434560
/estimated_length=163
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 436415..436963
/estimated_length=549
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 439668..439767
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 446744..446894
/estimated_length=151
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 447985..448554
/estimated_length=570
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 452639..453415
/estimated_length=777
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 453628..454891
/locus_tag="ANCCEY_05996"
mRNA join(453628..453703,453789..453926,454767..454891)
/locus_tag="ANCCEY_05996"
/product="hypothetical protein"
CDS join(453628..453703,453789..453926,454767..454891)
/locus_tag="ANCCEY_05996"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74934.1"
/translation="MQALWPYHTYRPVTWDKYYVNEYHEKQPTVSSGVAAGVTIGIFF
LVFLFTFGCRIYSQYADRSQAQNTRSQESVLSESLAEDMWICGVPQGPPPPYEVAIRM
PSSPPDASPV"
gene complement(455957..461500)
/locus_tag="ANCCEY_05997"
mRNA complement(join(455957..456125,458426..458595,
459149..459234,459341..459461,460878..461044,
461212..461320,461390..461500))
/locus_tag="ANCCEY_05997"
/product="HAD hydrolase, family IIA"
CDS complement(join(455957..456125,458426..458595,
459149..459234,459341..459461,460878..461044,
461212..461320,461390..461500))
/locus_tag="ANCCEY_05997"
/note="KEGG: cel:K09H11.7 3.8e-33 nitrophenylphosphatase;
hypothetical protein; K01101 4-nitrophenyl phosphatase"
/codon_start=1
/product="HAD hydrolase, family IIA"
/protein_id="EPB74935.1"
/translation="MSQSSGGGGAFTGVYHAFKEECNRYLQRKRIPVSSKGSKMFNMT
NTEARVEQLTAESFVDLLPEFDTFIFGGDGPMWLREGVITDVSHLANLLIEHKKEVFI
LTNDTNYSRQTHETKLSQHRFNKKLNKDRIVTPGLLAASYINANVTSTKKGVYLLSSG
GIQDDLKEHGINLFGPGPDALASLPNEGEMFTVDISVRSTDVCAVIIGLDRHLSFKKL
VKAASYLKNPKCLFLAINDNAVLGDPNEDVVIPDAGTVNCDVQFGRNHGMRTLLIASN
PHELDEMEKMRSSRCDALPHYYITSIPTILPQNK"
assembly_gap 457610..458096
/estimated_length=487
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 465739..465838
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 468902..469005
/estimated_length=104
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 473178..476736
/estimated_length=3559
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(483917..488179)
/locus_tag="ANCCEY_05998"
mRNA complement(join(483917..483984,484304..484566,
484619..484719,485343..485448,485520..485619,
485705..485840,485896..486011,486456..486585,
487820..487966,488060..488179))
/locus_tag="ANCCEY_05998"
/product="hypothetical protein"
CDS complement(join(483917..483984,484304..484566,
484619..484719,485343..485448,485520..485619,
485705..485840,485896..486011,486456..486585,
487820..487966,488060..488179))
/locus_tag="ANCCEY_05998"
/inference="protein motif:HMMPfam:IPR007145"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74936.1"
/db_xref="InterPro:IPR007145"
/translation="MVTAVASDIENGLNRIERMRSQLGMEPWENRKAPPGSVELLKSI
ESEIKKLRPLYEARQQEQEQLIEQLEQLSYRLGIEEENPARGCDDELLPTEKVRALEA
RRLRYNDMLQKRVRQASKWQKDMKKFAAVVGRSVDADDENIQTILNLDFTKDVCLSES
MMNSIESCYTQLYEMYSEHVQEKEFRWMELHTRLAELWELCHVADIERMIPSSYDPEK
HTEKDFERITTEISRLECLYEARKEVFDILTKWKSKWAEKMAIEEKKKSAEYFQNRGR
ENNVFMDAKIERTLSEFTLPKLLKSLIVAYDNYRESHPNDDIRVDGFTPPDYVKWVID
EYNASKEVERKTRVTCCTRLTRSRAFSVNSSLDSLADAAQSGVYKCVENAAVGSRKVA
AKTSELVQAGATSQAPSLRFSFHTESMLQFDDIIDT"
assembly_gap 487508..487686
/estimated_length=179
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 488720..488903
/estimated_length=184
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 506199..506320
/estimated_length=122
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 507836..507935
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 508918..509017
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 520861..520960
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 521916..523130
/estimated_length=1215
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 524953..525052
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 526402..527249
/locus_tag="ANCCEY_05999"
mRNA join(526402..526504,526913..526999,527047..527249)
/locus_tag="ANCCEY_05999"
/product="hypothetical protein"
CDS join(526402..526504,526913..526999,527047..527249)
/locus_tag="ANCCEY_05999"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB74937.1"
/translation="MMIAGHLESHHKCTSQSYCVSVKSRNGLVQRSCDGNSMAHISLC
ALSPVRRRVTNGDFIYGSSGTNGPNIPVPGAPNHHQTRNGRHQQLDTNCFNGGDLGEV
CCCSTDFCNSTSSARWIAVIAVLILFLG"
assembly_gap 528208..528942
/estimated_length=735
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 532962..533061
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(533702..536476)
/locus_tag="ANCCEY_06000"
mRNA complement(join(533702..533782,533847..533916,
534157..534235,535464..535605,535783..535949,
536006..536129,536474..536476))
/locus_tag="ANCCEY_06000"
/product="Ser/Thr protein phosphatase family protein"
CDS complement(join(533702..533782,533847..533916,
534157..534235,535464..535605,535783..535949,
536006..536129,536474..536476))
/locus_tag="ANCCEY_06000"
/inference="protein motif:HMMPfam:IPR004843"
/note="KEGG: dme:Dmel_CG16717 1.0e-23 CG16717 gene product
from transcript CG16717-RA"
/codon_start=1
/product="Ser/Thr protein phosphatase family protein"
/protein_id="EPB74938.1"
/db_xref="InterPro:IPR004843"
/translation="MYNDMYNAFLFQDIPDGDVLIHAGDLTNYGSERELKKVSEELGR
LPHKHKLIVAGNHDLGFDDSEDPNGRLEKYRGQGTPNGYLLLKNVTWLHDKGVEIEGV
KFFGSSWHPLYGYPFYRPRPELKEKWKVLPSDLDVLITHTPALGYLDTFGDERWGCRY
LLKEIEERVKPNFPGSMFLVTCTNGTELYPMELRYTFINAAQATKNNKIVNKPLVFYI
PKK"
CONTIG join(ASSL01022101.1:1..6526,gap(213),ASSL01022102.1:1..6478,
gap(unk100),ASSL01022103.1:1..14233,gap(389),
ASSL01022104.1:1..2365,gap(344),ASSL01022105.1:1..3033,gap(403),
ASSL01022106.1:1..1751,gap(1252),ASSL01022107.1:1..2007,gap(694),
ASSL01022108.1:1..10536,gap(1555),ASSL01022109.1:1..1211,
gap(unk100),ASSL01022110.1:1..13674,gap(unk100),
ASSL01022111.1:1..2700,gap(334),ASSL01022112.1:1..1063,gap(537),
ASSL01022113.1:1..735,gap(635),ASSL01022114.1:1..778,gap(747),
ASSL01022115.1:1..3368,gap(unk100),ASSL01022116.1:1..3166,gap(175),
ASSL01022117.1:1..696,gap(577),ASSL01022118.1:1..1443,gap(617),
ASSL01022119.1:1..2700,gap(663),ASSL01022120.1:1..2966,gap(228),
ASSL01022121.1:1..1744,gap(unk100),ASSL01022122.1:1..900,
gap(unk100),ASSL01022123.1:1..3950,gap(unk100),
ASSL01022124.1:1..14011,gap(unk100),ASSL01022125.1:1..7811,
gap(284),ASSL01022126.1:1..2186,gap(unk100),ASSL01022127.1:1..8462,
gap(499),ASSL01022128.1:1..2076,gap(1361),ASSL01022129.1:1..3721,
gap(unk100),ASSL01022130.1:1..2489,gap(131),ASSL01022131.1:1..1187,
gap(477),ASSL01022132.1:1..663,gap(492),ASSL01022133.1:1..5159,
gap(880),ASSL01022134.1:1..590,gap(unk100),ASSL01022135.1:1..551,
gap(672),ASSL01022136.1:1..1912,gap(469),ASSL01022137.1:1..1638,
gap(202),ASSL01022138.1:1..7819,gap(640),ASSL01022139.1:1..2457,
gap(746),ASSL01022140.1:1..1842,gap(unk100),ASSL01022141.1:1..4283,
gap(674),ASSL01022142.1:1..1670,gap(153),ASSL01022143.1:1..2284,
gap(unk100),ASSL01022144.1:1..4879,gap(189),ASSL01022145.1:1..1017,
gap(unk100),ASSL01022146.1:1..7776,gap(3524),
ASSL01022147.1:1..5551,gap(unk100),ASSL01022148.1:1..1005,gap(316),
ASSL01022149.1:1..3322,gap(unk100),ASSL01022150.1:1..11240,
gap(950),ASSL01022151.1:1..12092,gap(187),ASSL01022152.1:1..2184,
gap(648),ASSL01022153.1:1..16154,gap(242),ASSL01022154.1:1..1528,
gap(1245),ASSL01022155.1:1..6972,gap(unk100),
ASSL01022156.1:1..8873,gap(688),ASSL01022157.1:1..5454,gap(754),
ASSL01022158.1:1..15552,gap(1219),ASSL01022159.1:1..3262,gap(138),
ASSL01022160.1:1..2930,gap(1442),ASSL01022161.1:1..1526,gap(1143),
ASSL01022162.1:1..738,gap(1851),ASSL01022163.1:1..4076,gap(465),
ASSL01022164.1:1..3001,gap(unk100),ASSL01022165.1:1..2714,gap(777),
ASSL01022166.1:1..938,gap(432),ASSL01022167.1:1..7343,gap(unk100),
ASSL01022168.1:1..2492,gap(758),ASSL01022169.1:1..15922,gap(201),
ASSL01022170.1:1..2046,gap(333),ASSL01022171.1:1..8794,gap(unk100),
ASSL01022172.1:1..1161,gap(413),ASSL01022173.1:1..25552,gap(463),
ASSL01022174.1:1..7489,gap(unk100),ASSL01022175.1:1..8944,gap(440),
ASSL01022176.1:1..860,gap(124),ASSL01022177.1:1..4453,gap(633),
ASSL01022178.1:1..4001,gap(1033),ASSL01022179.1:1..1532,gap(430),
ASSL01022180.1:1..6375,gap(1090),ASSL01022181.1:1..775,gap(586),
ASSL01022182.1:1..11183,gap(163),ASSL01022183.1:1..1854,gap(549),
ASSL01022184.1:1..2704,gap(unk100),ASSL01022185.1:1..6976,gap(151),
ASSL01022186.1:1..1090,gap(570),ASSL01022187.1:1..4084,gap(777),
ASSL01022188.1:1..4194,gap(487),ASSL01022189.1:1..7642,gap(unk100),
ASSL01022190.1:1..3063,gap(104),ASSL01022191.1:1..4172,gap(3559),
ASSL01022192.1:1..10771,gap(179),ASSL01022193.1:1..1033,gap(184),
ASSL01022194.1:1..17295,gap(122),ASSL01022195.1:1..1515,
gap(unk100),ASSL01022196.1:1..982,gap(unk100),
ASSL01022197.1:1..11843,gap(unk100),ASSL01022198.1:1..955,
gap(1215),ASSL01022199.1:1..1822,gap(unk100),
ASSL01022200.1:1..3155,gap(735),ASSL01022201.1:1..4019,gap(unk100),
ASSL01022202.1:1..7729)
//