LOCUS KE124864 664832 bp DNA linear CON 06-JUN-2013
DEFINITION Ancylostoma ceylanicum unplaced genomic scaffold
A_ceylanicum-1.0_Cont90, whole genome shotgun sequence.
ACCESSION KE124864 ASSL01000000
VERSION KE124864.1
DBLINK BioProject: PRJNA72583
KEYWORDS WGS; HIGH_QUALITY_DRAFT.
SOURCE Ancylostoma ceylanicum
ORGANISM Ancylostoma ceylanicum
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae;
Ancylostomatinae; Ancylostoma.
REFERENCE 1 (bases 1 to 664832)
AUTHORS Mitreva,M.
TITLE Draft genome of the parasitic nematode Anyclostoma ceylanicum
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 664832)
AUTHORS Mitreva,M., Abubucker,S., Martin,J., Minx,P., Warren,C.,
Pepin,K.H., Palsikar,V.B., Zhang,X.W.E. and Wilson,R.K.
TITLE Direct Submission
JOURNAL Submitted (14-MAY-2013) The Genome Institute, Washington University
School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA
COMMENT Ancylostoma ceylanicum is a parasite of humans and carnivores in
Asia. The parasite was adapted to the Syrian golden hamster
(Mesocricetus auratus) in 1972 by Ray and Bhopale. The strain
(Indian) was distributed worldwide from the lab of Dr. Jerzy Behnke
in the 1980's. The sequenced strain was obtained by Dr. John M.
Hawdon (jhawdon@gwu.edu) from Dr. Ricardo Fujiwara at the Federal
University of Minas Gerais, Brazil. The strain was maintained in
Dr. Hawdon's lab in dogs and hamsters since 2007. Worm isolation
and extraction of nucleic acids was performed by Dr. Verena
Gelmedin and others in the Hawdon lab, or the Genome Institute
production team. Voucher specimens are on deposit in the U.S.
National Parasite Collection (accession number 102954). For the
original isolation and adaptation to hamsters see Ray, D.K.,
Bhopale, K.K., 1972. Complete development of Ancylostoma ceylanicum
(Looss, 1911) in golden hamsters, Mesocricetus auratus. Experientia
28, 359-361
This assembly consists of fragments, 3kb and 8kb insert whole
genome shotgun libraries. The sequences were generating on the
Roch/454 platform and assembled using Newbler. To improve
scaffolding, inhouse tools CIGA (Cdna tool for Improving Genome
Assembly) and Pygap (Gap closure tool) were used to map 454 cDNA
reads using blat to the genomic assembly to link genomic contigs
based on cDNA evidence. Only joins confirmed by additional
independent data typing were accepted and close gaps followed by
the Pyramid assembler and Illumina paired reads to closing gaps and
extending contigs
The repeat library was generated using Repeatmodeler (A.F.A. Smit,
R. Hubley & P. Green http://repeatmasker.org). The Ribosomal RNA
genes were identified using RNAmmer (Lagesen et. al., 2007 Nucleic
Acids Res.) and transfer RNA's were identified with tRNAscan-SE
(Lowe and Eddy, Nucleic Acids Res. 1997). Non-coding RNAs, such as
microRNAs, were identified by sequence homology search of the Rfam
database (Griffiths-Jones et. al., 2003 Nucleic Acids Res.).
Repeats and predicted RNA's were then masked using RepeatMasker (A.
Smit, R. Hubley & P. Green http://repeatmasker.org). Protein-coding
genes were predicted using a combination of ab initio programs Snap
(Korf, 2004 BCM Bioinformatics), Fgenesh (Salamov A., Solovyev V.
2000, Genome Res.) and Augustus (M. Stanke, et. al., 2008
Bioinformatics) and the annotation pipeline tool Maker (M. Yandell
et. al., 2007 Genomc Research) which aligns mRNA, EST and protein
information from same species or cross-species to aid in gene
structure determination and modifications. A consensus gene set
from the above prediction algorithms was generated, using a
logical, hierarchical approach developed at the Genome institute.
Gene product naming was determined by BER
(http://ber.sourceforge.net).
Our goal is to explore this WGS draft sequence of A. ceylanicum to
better define proteins involved in nematode parasitism that impact
health and disease and are relevant to both host-parasite
relationships and basic biological processes.
For information regarding this assembly or project, or any other
GSC genome project, please visit our Genome Groups web page
(http://genome.wustl.edu/genome_group_index.cgi) and email the
designated contact person. For specific questions regarding the A.
ceylanicum genome project contact Makedonka Mitreva
(mmitreva@genome.wustl.edu) at Washington University School of
Medicine. The National Human Genome Research Institute (NHGRI) of
the National Institutes of Health (NIH) provided funds for this
project.
##Genome-Assembly-Data-START##
Current Finishing Status :: High-Quality Draft
Assembly Method :: Newbler v.
MapAsmResearch-04/19/2010-patch-
08/17/2010
Assembly Name :: A_ceylanicum1.3.ec.cg.pg
Genome Coverage :: 26.10x
Sequencing Technology :: 454
##Genome-Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..664832
/organism="Ancylostoma ceylanicum"
/mol_type="genomic DNA"
/submitter_seqid="A_ceylanicum-1.0_Cont90"
/specimen_voucher="USDA:USNPC:102954"
/db_xref="taxon:53326"
/chromosome="Unknown"
gene <857..1923
/locus_tag="ANCCEY_04469"
mRNA join(<857..955,1017..1139,1345..1432,1736..1923)
/locus_tag="ANCCEY_04469"
/product="hypothetical protein"
CDS join(<857..955,1017..1139,1345..1432,1736..1923)
/locus_tag="ANCCEY_04469"
/inference="protein motif:HMMPfam:IPR013078"
/note="KEGG: cbe:Cbei_2798 3.1e-08 phosphoglycerate
mutase; K01834 phosphoglycerate mutase"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76411.1"
/db_xref="InterPro:IPR013078"
/translation="FSKVNLNHVFASPYDRTIETASIIVADKGLLVKPEPGLCEGLYM
CEKPPGFWEPEKLKEKFPLVDTDYISVFSKHTLPKEGFGDDACIPRVRTTLNRITEKY
DGDIMLVSHGAPIGAIHEIWMGEFKYVGQATVTKFIETEKGKIRMEFSSDASHLSDKR
NLRPW"
gene complement(2392..7442)
/locus_tag="ANCCEY_04470"
mRNA complement(join(2392..2490,3471..3629,3742..3874,
3973..4131,5734..5833,5901..6034,6102..6163,6337..6474,
6574..6707,7400..7442))
/locus_tag="ANCCEY_04470"
/product="Mov34/MPN/PAD-1 family protein"
CDS complement(join(2392..2490,3471..3629,3742..3874,
3973..4131,5734..5833,5901..6034,6102..6163,6337..6474,
6574..6707,7400..7442))
/locus_tag="ANCCEY_04470"
/inference="protein motif:HMMPfam:IPR000555"
/note="KEGG: tgo:TGME49_051500 2.0e-22 eukaryotic
translation initiation factor 3 subunit 3, putative
K03247"
/codon_start=1
/product="Mov34/MPN/PAD-1 family protein"
/protein_id="EPB76412.1"
/db_xref="InterPro:IPR000555"
/translation="MPKVMGFPHFRLRAGSIMSLAATISTITPPNVSYIQMDSLVIMK
IVKHVDSELYAGMSEVAGEACQGLLTGLVSVEKDDSRLEITNCFPTARAEPVLESDEN
AAQANQMYEELKQQEMLDMLRKFRNMNIDYELVGFYQAHPFGACFSQDLVDSMFDYQS
NGPDGVVIIYDPVKTRQGQLCMRAYRLSVPALELCAKNDWSPDAVKAANLTYQTMFEE
LPIVIKSSHLVNVMMAELSLAPTRIADRFSTHLELGSRRSLEKSVRAMMANIDELNKS
ISAYGKYVNDKQRHDNMIYNLTQKRQAENEQRIARGEPPLSMDDIRKQKEPQLQTRNG
MLDAMLSSCETQALADFCVKVTGENIAKLFLAEALADDKGTTKDRSQSLLSR"
assembly_gap 4529..4954
/estimated_length=426
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 8984..12407
/locus_tag="ANCCEY_04471"
mRNA join(8984..9036,10510..10708,12035..12124,12179..12265,
12327..12407)
/locus_tag="ANCCEY_04471"
/product="hypothetical protein"
CDS join(8984..9036,10510..10708,12035..12124,12179..12265,
12327..12407)
/locus_tag="ANCCEY_04471"
/note="KEGG: phu:Phum_PHUM167510 4.8e-26 Stromal
cell-derived factor 2 precursor, putative"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76413.1"
/translation="MTIDVKLVRSAGTLPIVTTAMSWFIVLVVSSCAVIGFCYDDEVT
CGTVLKLMNINEGSRLHSHEVKYGSGSGQQSVTAVQASDDEVSCFGNDAQSDTGDNWQ
VVCDTEEWLETEPVKFKHVDTGVYLALSGQQFGRPIHGQREVVGTDGATSGGKWKAAE
GVFMKRNKE"
assembly_gap 9684..10022
/estimated_length=339
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(19168..25526)
/locus_tag="ANCCEY_04472"
mRNA complement(join(19168..19500,20439..20518,20581..20710,
20796..20962,21043..21149,23024..23124,23392..23512,
23582..23655,23947..24032,25406..25526))
/locus_tag="ANCCEY_04472"
/product="zinc finger, C4 type"
CDS complement(join(19168..19500,20439..20518,20581..20710,
20796..20962,21043..21149,23024..23124,23392..23512,
23582..23655,23947..24032,25406..25526))
/locus_tag="ANCCEY_04472"
/inference="protein motif:HMMPfam:IPR000536"
/inference="protein motif:HMMPfam:IPR001628"
/codon_start=1
/product="zinc finger, C4 type"
/protein_id="EPB76414.1"
/db_xref="InterPro:IPR000536"
/db_xref="InterPro:IPR001628"
/translation="MSSARVELSSTSVAVGAIIIKKEEPSSTSTTPHADGEENQPQIE
VIPCKVCGDKSSGVHYGVITCEGCKGFFRRSQSSVTNYQCPRQKNCTVDRVNRNRCQY
CRLKKCMELGMSRDAVKFGRMSKKQREKVEDEVRLHKQMQEATGIPYMYGEYSPPSQQ
PSTYAAPGYETGLYPHYAQGAYAHAVAAAPISYPSHSYGVAQQGAVPASNGGFPSPQL
SSAAEEDTAAKIVAAFETTHTQMRNMNPNPTIVDVNRFASINRVGIWRQFAADLTTVI
QAIIEFAKMIDGFMRLGQEEQIHLLKGGVFELAVVELSSRYSPETQTLSMDNIVRFVQ
EFHQCLHDLAVCQLTSSEVALLCAILLLEPASTEQATCERLRTVLAQSLAARVGSDAQ
RVWDTLPRLKQTAHLHLILLGQLRAQFPAETEAGLPALYKELFSIDP"
assembly_gap 20187..20286
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 26756..26855
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 38688..39183
/estimated_length=496
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 41234..41647
/estimated_length=414
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 42694..42793
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 48920..49392
/estimated_length=473
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 52108..52316
/estimated_length=209
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(<52472..56020)
/locus_tag="ANCCEY_04473"
mRNA complement(join(<52472..52588,52645..52749,52800..52946,
54099..54241,54325..54783,54866..55034,55877..56020))
/locus_tag="ANCCEY_04473"
/product="translation elongation factor EF-1, subunit
alpha"
CDS complement(join(<52472..52588,52645..52749,52800..52946,
54099..54241,54325..54783,54866..55034,55877..56020))
/locus_tag="ANCCEY_04473"
/inference="protein motif:HMMPfam:IPR000795"
/inference="protein motif:HMMPfam:IPR004160"
/inference="protein motif:HMMPfam:IPR004161"
/inference="protein motif:HMMTigr:IPR004539"
/note="KEGG: cbr:CBG16491 2.3e-209 Cbr-eft-3.1; C.
briggsae CBR-EFT-3.1 protein; K03231 elongation factor
EF-1 alpha subunit"
/codon_start=1
/product="translation elongation factor EF-1, subunit
alpha"
/protein_id="EPB76415.1"
/db_xref="InterPro:IPR000795"
/db_xref="InterPro:IPR004160"
/db_xref="InterPro:IPR004161"
/db_xref="InterPro:IPR004539"
/translation="MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK
EAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYITIIDAPGHRDFIKNM
ITGTSQADCAVLVVACGTGEFEAGISKNGQTREHALLAQTLGVKQLIVACNKMDSTEP
PFSEARYNEITTEVSNFIKKIGYNPKAVAFVPISGFNGDNMLEPSSNMPWFKGWNVER
KEGNATGKTLLEALDAIIPPQRPTDRPLRLPLQDVYKIGGIGTVPVGRVETGILKPGM
VVTFAPQNVTTEVSNFIKKIGYNPKAVAFSLPEAVPGDNVGFNVKNVSVKDIRRGSVC
SDSKNDPAKEARTFNAQVGEVIIMNHPGQIAAGYTPVLDCHTAHIACKFAELKEKVDR
RTGKKVEDNPKFLKSGDAGIVELIPTKPLCVESFTD"
assembly_gap 52965..54002
/estimated_length=1038
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 60248..60439
/estimated_length=192
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(60871..67142)
/locus_tag="ANCCEY_04474"
mRNA complement(join(60871..60972,61045..61179,61667..61786,
62092..62186,66489..66606,66857..66916,67056..67142))
/locus_tag="ANCCEY_04474"
/product="SH3 domain protein"
CDS complement(join(60871..60972,61045..61179,61667..61786,
62092..62186,66489..66606,66857..66916,67056..67142))
/locus_tag="ANCCEY_04474"
/inference="protein motif:HMMPfam:IPR002110"
/inference="protein motif:HMMPfam:IPR011511"
/note="KEGG: spu:584303 5.9e-13 similar to Tankyrase,
TRF1-interacting ankyrin-related ADP-ribose polymerase;
K10799 tankyrase"
/codon_start=1
/product="SH3 domain protein"
/protein_id="EPB76416.1"
/db_xref="InterPro:IPR002110"
/db_xref="InterPro:IPR011511"
/translation="MAAAAPPRPAPKPGRVRVYRALYDYDARTKSSSTYQRAKRSTMD
DSRKEDQEMSFTEGDLLYVSDRGPNDDWLPATCGGRKGLVPANYVVSENVEELSNPLH
EAARRGNLLFLKECIGNQVSVNSLDKSGSTALYWACHGGHVEVVKYLLQLPAVTISSQ
NKLGDTPLHAASWKGHKECVKLMIESGANTWIRNRDQKLPIDVAKDAEIAGLIDQAMR
RDTKNEDCDNEYESGSDKED"
assembly_gap 62393..63115
/estimated_length=723
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 64958..65652
/estimated_length=695
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(71248..86645)
/locus_tag="ANCCEY_04475"
mRNA complement(join(71248..71358,74989..75091,75601..75701,
76005..76077,76144..76229,76818..76940,77007..77124,
79951..80063,80179..80298,81543..81700,81796..81952,
82035..82147,82232..82344,82680..82822,84239..84343,
84408..84450,84536..84690,86264..86307,86519..86645))
/locus_tag="ANCCEY_04475"
/product="Pyrimidine 5'-nucleotidase"
CDS complement(join(71248..71358,74989..75091,75601..75701,
76005..76077,76144..76229,76818..76940,77007..77124,
79951..80063,80179..80298,81543..81700,81796..81952,
82035..82147,82232..82344,82680..82822,84239..84343,
84408..84450,84536..84690,86264..86307,86519..86645))
/locus_tag="ANCCEY_04475"
/note="KEGG: cin:100184046 3.8e-27 similar to Cytosolic
5-nucleotidase III (cN-III) (Pyrimidine 5-nucleotidase 1)
(P5N-1) (P5N-1) (PN-I); K01081 5'-nucleotidase"
/codon_start=1
/product="Pyrimidine 5'-nucleotidase"
/protein_id="EPB76417.1"
/translation="METEVIVLDSDDDDSPANAPVNGSALASIAIVNSKPLDLDALAN
RIKSSTGVPYLQTITPIAPAPQRQRLSYEKTRSQPPCEDFGINAISLLGNTASPRPLQ
PRPTTQMHSRPANVSPSFRPAPRLVPESRVQNYNFEVNGEKSTDSDRQLCPSLKFKVG
TASALRTHLLAHEPNDAPYHCKRCRYRCTVRMHLFDHFVREHKNTSTLMCPFCTFTIV
VPSYQRKRPVIRASEFVYHMISHEAEARLGCDHCALSFSTLSEKQKHRKEHTAVNPKW
TLTYRNPETRTRPRSRLVCPEQKPLYQCSKCACSSETGTSHKKCISCVTLAFNESLYG
KRRAATDTSLRRGIDSLQCSTRGMRFRCKCGLSTRNGNRMALHYYYCHKDFDVVETDD
ELGDQSETDEAEDKQEAQLFSVLHKVEPVVKAREKTVERKRKFLEPPLMIFEQQMNSM
SKPEDMNNAVVFFLRIVMVVEKILNHPSVRVRDRSAVVEKLNAILKGGNEQLAVISDF
DFTLTKSIDEKGERCLGSHAVVNHLLLSLHPELAAEVNAVNAKYVAIEYDPHLTKEEK
MPYMVEWWTKAHENYITHGIHRDDIERIVEEGRVNACSEPLIHVFCKDSSVIPHDAPF
FDDIAHRGNVLLLGDSLGDIHMDVGVDHSGTVLRIGYLNSNAEKLLELYLDGFDIVLV
EDQTTDVPDLILQALVKQC"
assembly_gap 71826..74218
/estimated_length=2393
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 80821..80920
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 86360..86476
/estimated_length=117
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 87660..88605
/estimated_length=946
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(89687..95239)
/locus_tag="ANCCEY_04476"
mRNA complement(join(89687..89713,94826..95239))
/locus_tag="ANCCEY_04476"
/product="hypothetical protein"
CDS complement(join(89687..89713,94826..95239))
/locus_tag="ANCCEY_04476"
/note="KEGG: oaa:100084025 4.5e-09 hypothetical protein
LOC100084025; K04407 mitogen-activated protein kinase
kinase kinase kinase 4"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76418.1"
/translation="MENDFPEPRRTSGKTYSDSLSAVKRRLQRANETEQEKYERRFEA
RRRALLKRLNESEEQRENRLGRERVRMRLRRADETREQTMKRRQENLERMRVKRALES
EEERMDRRRRERERKRIRRHLEAARSRIFGAGDETRGISGISSI"
assembly_gap 90971..91951
/estimated_length=981
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 93101..93375
/estimated_length=275
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 96091..96400
/estimated_length=310
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(97496..104247)
/locus_tag="ANCCEY_04477"
mRNA complement(join(97496..97560,97626..97824,100367..100456,
101362..101437,101499..101605,101677..101791,
104207..104247))
/locus_tag="ANCCEY_04477"
/product="zona pellucida-like domain protein"
CDS complement(join(97496..97560,97626..97824,100367..100456,
101362..101437,101499..101605,101677..101791,
104207..104247))
/locus_tag="ANCCEY_04477"
/inference="protein motif:HMMPfam:IPR001507"
/codon_start=1
/product="zona pellucida-like domain protein"
/protein_id="EPB76419.1"
/db_xref="InterPro:IPR001507"
/translation="MEPDSIVTQSIDVSSLPTTELADEMQMPKCEYSVRRDSPNGPTL
TFANVGETVYHVWECNPPDMGMLVKKCFVTDGDGEDHAVIDYDGCSTDVSLLSELAYD
QNLMKAHATSQVFKYADSNQLYFTCQIRLCQRQMGMCQDVTPPFCGANRPNRTRRAIA
RTQDPEIDVATREMLILDSEERRQMGSSPLCVPKMTLPLIPLVLISVSAVSVTATLLV
VSRKRDKIPYEA"
gene complement(105845..110079)
/locus_tag="ANCCEY_04478"
mRNA complement(join(105845..105850,105877..105916,
107063..107133,107653..107738,110067..110079))
/locus_tag="ANCCEY_04478"
/product="hypothetical protein"
CDS complement(join(105845..105850,105877..105916,
107063..107133,107653..107738,110067..110079))
/locus_tag="ANCCEY_04478"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76420.1"
/translation="MIVSCTPKVMCEDNDLALDIVTEKPFQGNIFVKSIPLGKASNLS
WTSANLIYNVDLIMSKSDFFKALAKMS"
gene 106355..106426
/locus_tag="ANCCEY_16223"
tRNA 106355..106426
/locus_tag="ANCCEY_16223"
/product="tRNA-Ala"
assembly_gap 109427..109526
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 114719..114818
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 117248..117347
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 119389..119699
/estimated_length=311
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 123054..125808
/locus_tag="ANCCEY_04479"
mRNA join(123054..123147,123741..123856,123973..124049,
124937..125054,125154..125443,125631..125808)
/locus_tag="ANCCEY_04479"
/product="hypothetical protein"
CDS join(123054..123147,123741..123856,123973..124049,
124937..125054,125154..125443,125631..125808)
/locus_tag="ANCCEY_04479"
/inference="protein motif:HMMPfam:IPR000571"
/note="KEGG: smm:Smp_165400.1 1.7e-10 acetyl-CoA
carboxylase; methylcrotonyl-CoA carboxylase"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76421.1"
/db_xref="InterPro:IPR000571"
/translation="MSRSTHEQFYYGIDDQARRPAVRPIPENRQKGFHGQTRLITVTP
DAIIPSINEPIEASGGSRRRRSDLFDTRGRRQCVTWETMTDEEREEVQRMKRKDEAYK
TALCDAFKKFGFCPYGEGCRFAHGDSELRLPAQPRGKAHPKYKTQLCDKFSTFGHCPY
GPRCQFIHKLKKVMLLPTCAGLLRLHMRFQGLPLIQYERLLAAGQISPEREDESRVTP
ASQSRRSSATSPGRNRADAARRLSFDRENSFTPRQKIPNAIYPKMEIINIERACKYAD
EIVEKSEFGDCSYS"
assembly_gap 124710..124809
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 126776..127055
/estimated_length=280
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 133676..136982
/estimated_length=3307
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 142534..145680
/locus_tag="ANCCEY_04480"
mRNA join(142534..142721,144707..144945,145580..145680)
/locus_tag="ANCCEY_04480"
/product="hypothetical protein"
CDS join(142534..142721,144707..144945,145580..145680)
/locus_tag="ANCCEY_04480"
/note="KEGG: ssl:SS1G_04405 0.0014 hypothetical protein;
K12858 ATP-dependent RNA helicase DDX23/PRP28"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76422.1"
/translation="MLQIWDRRCDIPAADRNLGETKDDAVVAGIVLASIHRAQRGLEI
LCDLNVICHRVRDLRGQIRSPSPSPPPPPPPPDDAASRRRNRDKSETPPPPPPPLPLI
NEFALERQQRKLREAEKRVAEARSKRARSPPKKKSKTNNDGMSEKERRKRELMEQLRA
VEAELKKRTAATSAM"
gene 148552..151034
/locus_tag="ANCCEY_04481"
mRNA join(148552..148691,149295..149445,149498..149573,
150892..151034)
/locus_tag="ANCCEY_04481"
/product="hypothetical protein"
CDS join(148552..148691,149295..149445,149498..149573,
150892..151034)
/locus_tag="ANCCEY_04481"
/inference="protein motif:HMMPfam:IPR002730"
/note="KEGG: cel:C15C6.4 1.2e-38 hypothetical protein;
K03538 ribonuclease P subunit P29"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76423.1"
/db_xref="InterPro:IPR002730"
/translation="MKNQTEEQRLGVLHLDKTHRCKRNPKKFRKTNFTRSALTEEDKR
ALKYEQVEPLYQMWCEYYRSLLGDQQKTPDERMLKADYHGALVLVAEAHNTTMIGKVG
IIVLETRQTFQLITKENKYAVIPKQGTALQFILDGRVFTLFGDAMRYKPSLRGKKHRL
RVALPFFIR"
assembly_gap 152021..152198
/estimated_length=178
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 153095..154344
/estimated_length=1250
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 163418..163888
/estimated_length=471
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 166311..167428
/estimated_length=1118
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 168697..170664
/estimated_length=1968
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 175682..175850
/estimated_length=169
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(177676..>178896)
/locus_tag="ANCCEY_04482"
mRNA complement(join(177676..177808,177873..177973,
178031..178144,178726..>178896))
/locus_tag="ANCCEY_04482"
/product="DB module"
CDS complement(join(177676..177808,177873..177973,
178031..178144,178726..>178896))
/locus_tag="ANCCEY_04482"
/inference="protein motif:HMMPfam:IPR002602"
/codon_start=1
/product="DB module"
/protein_id="EPB76424.1"
/db_xref="InterPro:IPR002602"
/translation="TFSGGDRKLLNQEYPITEVTDADLTAECGTEKSRPPYSPCLARK
TVDDVFKSCCQQHHEFQVPANCHSLCTYEHREHVAAETLIAAVQQDGCDLKYLSSILY
CANQNRDNRKCCEFLGMSNSELGVGDRCLRLCNVAPSGDRVSSVEKSDLVCLSNWNVM
MYCARSGLRTFN"
assembly_gap 185736..187195
/estimated_length=1460
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(188347..190102)
/locus_tag="ANCCEY_04483"
mRNA complement(join(188347..188352,188654..188744,
189999..190102))
/locus_tag="ANCCEY_04483"
/product="hypothetical protein"
CDS complement(join(188347..188352,188654..188744,
189999..190102))
/locus_tag="ANCCEY_04483"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76425.1"
/translation="MLPDSPSPRAALALSDLQPQTGASTFAVGTGSGNWSTHGVGGVG
GDSFMDKNSNGGGLFGNNRQND"
assembly_gap 197448..197547
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 203412..204175
/estimated_length=764
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 216741..217347
/estimated_length=607
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 226291..226532
/estimated_length=242
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 234714..235437
/estimated_length=724
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 244816..245006
/estimated_length=191
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(247454..248108)
/locus_tag="ANCCEY_04484"
mRNA complement(join(247454..247530,247587..247701,
247758..248108))
/locus_tag="ANCCEY_04484"
/product="hypothetical protein"
CDS complement(join(247454..247530,247587..247701,
247758..248108))
/locus_tag="ANCCEY_04484"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76426.1"
/translation="MTARRSSIIGHQSISSRAASIADDDDDDGFYDNIGTVRGTCSPV
PAKEKLRNAKPGILQFDDRRFSRGSEMDVASMSSHQLPPHGWKPTKIGSFLRKIGGGG
SRPPGSAASLVSLNKQVANETSIKSGNLMKSNSLSTEPWKKMVIDGPSLPTRESTPSK
GGFGARIKNSIFGSKKRLNG"
assembly_gap 248594..248693
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 252872..253250
/estimated_length=379
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(253713..253961)
/locus_tag="ANCCEY_04485"
mRNA complement(join(253713..253887,253945..253961))
/locus_tag="ANCCEY_04485"
/product="hypothetical protein"
CDS complement(join(253713..253887,253945..253961))
/locus_tag="ANCCEY_04485"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76427.1"
/translation="MRGSLRSLPDAGLIIRGKYHQVTPPKDPTLAIDQLCAELELNTD
QVTVWVAVYPGFLIWVKVA"
gene complement(263184..269731)
/locus_tag="ANCCEY_04486"
mRNA complement(join(263184..263268,265594..265679,
266035..266179,269727..269731))
/locus_tag="ANCCEY_04486"
/product="hypothetical protein"
CDS complement(join(263184..263268,265594..265679,
266035..266179,269727..269731))
/locus_tag="ANCCEY_04486"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76428.1"
/translation="MNGALDSATKQLDQVIDQARHKHSQHRNKFKEAIDYLDQIFEDL
KKEVDPGVEDKIKDRSVAAAAPQHSTVTSPPDNIRFINNWHNISATSSAPSEKHDDIS
CSSL"
assembly_gap 270390..270489
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(277378..284066)
/locus_tag="ANCCEY_04487"
mRNA complement(join(277378..277426,280103..280250,
284024..284066))
/locus_tag="ANCCEY_04487"
/product="hypothetical protein"
CDS complement(join(277378..277426,280103..280250,
284024..284066))
/locus_tag="ANCCEY_04487"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76429.1"
/translation="MALVLFQCIAVSLPGMTSLSTSYTAAIAANTNPARHERYAAHIP
VNKGDSSGRQSTVSQDSQHRLVNLQFDTPLLFLVF"
assembly_gap 279245..279486
/estimated_length=242
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 285789..286037
/estimated_length=249
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 286760..287238
/estimated_length=479
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 296835..296934
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 302517..302616
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 307527..307626
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 313122..313469
/estimated_length=348
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(315434..319245)
/locus_tag="ANCCEY_04488"
mRNA complement(join(315434..315748,317318..317555,
319199..319245))
/locus_tag="ANCCEY_04488"
/product="hypothetical protein"
CDS complement(join(315434..315748,317318..317555,
319199..319245))
/locus_tag="ANCCEY_04488"
/note="KEGG: tca:100142337 1.4e-09 similar to chromodomain
helicase-DNA-binding protein 3; K11643
chromodomain-helicase-DNA-binding protein 4"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76430.1"
/translation="MTKVNTVLWFTSNQIRDQEEQGVIHVWAARNRASVTKRTKATPR
KVAAPKPALNKPAETKQDQMKKDDKKEMKENIPAEDKDQKKSTGPSKFKKLFTAKTRP
VVEPAKDEKTEVKVEAKQAAGDKKKEVERKEAIEPKEVKKPEPPKKTQAKSEPQKDEK
PVVASPATKKDEGKVEKVVPKPRILKEEKTQVGIIMFTT"
assembly_gap 319967..320066
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 321314..321413
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 327614..329757
/estimated_length=2144
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 333164..333798
/estimated_length=635
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(336105..354036)
/locus_tag="ANCCEY_04489"
mRNA complement(join(336105..336178,336690..337116,
340222..340369,353939..354036))
/locus_tag="ANCCEY_04489"
/product="hypothetical protein"
CDS complement(join(336105..336178,336690..337116,
340222..340369,353939..354036))
/locus_tag="ANCCEY_04489"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76431.1"
/translation="MRVEELGGDPEASHPTRVKPEITSSTVKNSKSGVSFAVRWMDLR
TAVELRQPPESLQPMRKVISITFVSRHDFESRAAAGNKLELSTLEQTFKHGLSKPLGS
DRVAIRAEALLLDVAGVAHRSVAVLDGDQRSGEGRRSSGQVSMKNDDLLTGWRLFVAA
PRDRERDQSALSQRDRLRFSHFSLGPSVSARASRFDSSMRDGGNKYRNEAREILNKWT
SRERNGACMEMPHIKDGHALLTPLSPLFNH"
assembly_gap 343395..343503
/estimated_length=109
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 351709..351890
/estimated_length=182
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 364285..364384
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 365484..365583
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 381872..382222
/estimated_length=351
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 387517..387810
/estimated_length=294
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 390533..392645
/estimated_length=2113
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 396698..396797
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 400674..401408
/estimated_length=735
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 404601..405232
/estimated_length=632
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 405880..405979
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 406756..406987
/estimated_length=232
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 408226..408913
/estimated_length=688
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 413654..414227
/estimated_length=574
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 417634..417850
/estimated_length=217
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 420709..421461
/estimated_length=753
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 427917..428016
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 430315..430859
/estimated_length=545
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 431093..434123
/locus_tag="ANCCEY_04490"
mRNA join(431093..431148,432765..432920,433584..433722,
433950..434123)
/locus_tag="ANCCEY_04490"
/product="Neurotransmitter-gated ion-channel ligand
binding domain protein"
CDS join(431093..431148,432765..432920,433584..433722,
433950..434123)
/locus_tag="ANCCEY_04490"
/inference="protein motif:HMMPfam:IPR006202"
/codon_start=1
/product="Neurotransmitter-gated ion-channel ligand
binding domain protein"
/protein_id="EPB76432.1"
/db_xref="InterPro:IPR006202"
/translation="MLTAVLAFVLNLAWAKELGYEKLLDEQRIIRNLLENPQSDYDWR
VRPRGRLNEPDYVDADPVIITVNMYLRSISKVDDVNMEYSLHFTFREEWVDERLYFNS
PTLKHIVLSPGQRIWVPDTFFQNEKDGKKHDIDTPNILIRVHNGTARILYSCRLTLTL
SCPMKYEQSVLLPY"
assembly_gap 452102..452672
/estimated_length=571
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(455425..455691)
/locus_tag="ANCCEY_04491"
mRNA complement(455425..455691)
/locus_tag="ANCCEY_04491"
/product="hypothetical protein"
CDS complement(455425..455691)
/locus_tag="ANCCEY_04491"
/inference="protein motif:HMMPfam:IPR000477"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76433.1"
/db_xref="InterPro:IPR000477"
/translation="MRRLEWDSMGVRVDSGLLHHLRFADDIVLITPSISQVERMLADF
DDACGKIGPQLNLTKTMFMRNGWVPNAPFSLNGTTISECPATYI"
assembly_gap 458863..462427
/estimated_length=3565
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 466290..466389
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 468357..468456
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 473199..473582
/locus_tag="ANCCEY_04492"
mRNA join(473199..473399,473463..473582)
/locus_tag="ANCCEY_04492"
/product="hypothetical protein"
CDS join(473199..473399,473463..473582)
/locus_tag="ANCCEY_04492"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76434.1"
/translation="MYCASRFSKLKADWAGRSDEGGEACPSATKIQVMSIRIGGDKSR
LTGLRSRHTYSPPQLPMLAQLQLILLFICLFLLSLPSEACLGGLGGGGACCGQPACPT
PCGK"
assembly_gap 475192..475535
/estimated_length=344
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 478858..479350
/estimated_length=493
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 488114..488213
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 490029..490128
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 493297..493396
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 496682..497703
/estimated_length=1022
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 504024..504316
/estimated_length=293
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 506610..507325
/estimated_length=716
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(510493..511871)
/locus_tag="ANCCEY_04493"
mRNA complement(join(510493..510630,510840..511109,
511609..511758,511821..511871))
/locus_tag="ANCCEY_04493"
/product="NAC domain protein"
CDS complement(join(510493..510630,510840..511109,
511609..511758,511821..511871))
/locus_tag="ANCCEY_04493"
/inference="protein motif:HMMPfam:IPR000449"
/inference="protein motif:HMMPfam:IPR002715"
/note="KEGG: isc:IscW_ISCW012592 9.8e-51 transcription
factor containing NAC and TS-N domains, putative K03626"
/codon_start=1
/product="NAC domain protein"
/protein_id="EPB76435.1"
/db_xref="InterPro:IPR000449"
/db_xref="InterPro:IPR002715"
/translation="MVAKAEVEAAKKEEMEKVVVEDGSDDSDDDAPGLDGELTEEQKK
VAEAAGLGDQMDKQVKQSRSEKKARKLFSKLGLKPVTGVSRVCIRKSKNILFVINKPD
VYKSPGSDTYIVFGEAKIEDLSQHQQLTAIDRIKPSEDTPAPLATVQEDEDDGTEEDA
TGIEEKDIELVMSQANTSRNKAIRALRSADNDIVNAIMSLTM"
gene complement(512377..>519403)
/locus_tag="ANCCEY_04494"
mRNA complement(join(512377..513147,513512..513671,
513739..513899,513971..514178,515850..515992,
516470..516604,516933..517097,517220..517312,
519140..>519403))
/locus_tag="ANCCEY_04494"
/product="HECT-domain protein"
CDS complement(join(512377..513147,513512..513671,
513739..513899,513971..514178,515850..515992,
516470..516604,516933..517097,517220..517312,
519140..>519403))
/locus_tag="ANCCEY_04494"
/inference="protein motif:HMMPfam:IPR000008"
/inference="protein motif:HMMPfam:IPR000569"
/inference="protein motif:HMMPfam:IPR001202"
/note="KEGG: cel:Y65B4BR.4 0. wwp-1; WW domain Protein (E3
ubiquitin ligase) family member (wwp-1); K05633 atrophin-1
interacting protein 5 (WW domain containing E3 ubiquitin
protein ligase 1)"
/codon_start=1
/product="HECT-domain protein"
/protein_id="EPB76436.1"
/db_xref="InterPro:IPR000008"
/db_xref="InterPro:IPR000569"
/db_xref="InterPro:IPR001202"
/translation="LFTLTFAVISAKLSPSGGFLSKPVDSYVEVSIEGSQASKKTSVK
KKSNNPEWDESLSLPVTDSSVLLFRVYSRAKLFDDPLIAQAQMKGVDGEASTSSGVMA
RRSSVKNRDTIAQTQQQPSAEDQRLPDGWEMRYDQYGRKYYVDHTTKSTTWERPSTQP
LPSGWEMRRDPRGRVYYVDHNTRTTTWQRPTADMLEAHEQWQSGRDQAMHQWEQRFLL
QANSLTASDDPLGPLPDGWEKREDPKTRRIYFVNHVNRTTQWEDPRTQGVSDQPLPDG
WEMRFTEQGVPFFIDHSTKTTTYNDPRTGKPVGPMGVFGVSMSYERTFRWKIAQFRYL
CLSNSVPNHVKIAVSRTNVFEDSFQEIMRKNAVDLRRRLYIQFRGEEGLDYGGVAREW
FFLLSHEVLNPMYCLFMYAGNSNYSLQINPASFVNPDHLKYFEYIGRFIAMALFHGKF
IYSGFTMPFYKKMLSKKIALKDIEQVDTEFYNSVMWIKDNNIDECQMELYFVADYELL
GELKTHELKPGGTDIPVTEANKLEYISLLVEWRFNRGVEQQTKAFFTGFNSVFPLEWL
QYFDERELELLLCGMQDVDVDDWQRNTVYRHYAPQSKQVVWFWQWVRNLDQEKRARLL
QFVTGTCRVPVGGFSELMGSTGPQLFCIERVGKENWLPRSHTCFNRLDLPPYRSYEQL
SEKLNMAIEMTEGFGNE"
assembly_gap 514354..514505
/estimated_length=152
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 520818..521279
/estimated_length=462
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(522038..525311)
/locus_tag="ANCCEY_04495"
mRNA complement(join(522038..522448,522940..523046,
525206..525311))
/locus_tag="ANCCEY_04495"
/product="partner of SLD five, PSF3"
CDS complement(join(522038..522448,522940..523046,
525206..525311))
/locus_tag="ANCCEY_04495"
/inference="protein motif:HMMPfam:IPR021151"
/codon_start=1
/product="partner of SLD five, PSF3"
/protein_id="EPB76437.1"
/db_xref="InterPro:IPR021151"
/translation="MAANEVEVPSDFYDIDSLLSRSTRVTCTYSSGTPRDVYDMLGTT
APHFTDEKGYKVLTPFWFVRALEPFCSLVLPMAYNSNVRNVLLANAAHADLEALQPYY
YEMGMHLCNSLNETVSIALAECLLKTIVQRIGGKPTCRLKLGFAMPNNFAGFIGIVLR
TIHGNEAPRRIDNLEKKLYEESAKNRDRLQDYFRKQRSTKGRKRRYE"
gene 526319..531133
/locus_tag="ANCCEY_04496"
mRNA join(526319..526390,526918..527051,527106..527288,
527740..527896,528998..529016,529810..529948,
530048..530138,530655..530745,530821..530891,
530954..531133)
/locus_tag="ANCCEY_04496"
/product="papain family cysteine protease"
CDS join(526319..526390,526918..527051,527106..527288,
527740..527896,528998..529016,529810..529948,
530048..530138,530655..530745,530821..530891,
530954..531133)
/locus_tag="ANCCEY_04496"
/inference="protein motif:HMMPfam:IPR000668"
/inference="protein motif:HMMPfam:IPR013201"
/note="KEGG: cin:100175491 1.7e-49 similar to predicted
protein; K01373 cathepsin F"
/codon_start=1
/product="papain family cysteine protease"
/protein_id="EPB76438.1"
/db_xref="InterPro:IPR000668"
/db_xref="InterPro:IPR013201"
/translation="MWINFLWEFIYIPKEQLGKDYVERAALRPSPPSKPASLVMWPKL
TKKSKDIDFERLEISEGEDVPSKKRTWFIPVLYQLVDLVAVVVQSVLVGAVIYLFLKM
SFILYNRVFAAVFQELIPSGSGIHDSQFYNKTYTDEAERDFRFSVFITNVRYFEEEEK
KHPGLDLDVTQFADWTEEEMKKVSSKNISQCGSCWAFATAASVEAGHAIKTGRLTTLS
EQEMVDCDTRNNGCNGGFVKEHGLMKEAEYPYRGTDDNMCLLTKNSTDRVFIRDYRTL
STNEEVVADWIAANGPATFGMNVTKALYSYRSGVFTPSKEDCEEHSLGSHALTIVGYG
AEGGKPFWLVKNSWGSRWGQGGYFKLARGVNACGAANNVVAPIL"
gene complement(532676..537146)
/locus_tag="ANCCEY_04497"
mRNA complement(join(532676..532789,532877..532964,
533027..533144,533221..533315,533368..533578,
533644..533797,533877..534039,534101..534180,
534581..534745,534894..535037,535098..535194,
535258..535343,535401..535496,535555..535661,
535717..535846,535913..536032,536773..536887,
536982..537055,537111..537146))
/locus_tag="ANCCEY_04497"
/product="homeobox domain protein"
CDS complement(join(532676..532789,532877..532964,
533027..533144,533221..533315,533368..533578,
533644..533797,533877..534039,534101..534180,
534581..534745,534894..535037,535098..535194,
535258..535343,535401..535496,535555..535661,
535717..535846,535913..536032,536773..536887,
536982..537055,537111..537146))
/locus_tag="ANCCEY_04497"
/inference="protein motif:HMMPfam:IPR001356"
/note="KEGG: phu:Phum_PHUM582360 5.1e-09 LIM/homeobox
protein Lhx2, putative K09373"
/codon_start=1
/product="homeobox domain protein"
/protein_id="EPB76439.1"
/db_xref="InterPro:IPR001356"
/translation="MGEFFNPLVAQQMQQLSSSPAKRARTRITDDQLKILRQYFDINN
SPSESQIKEMSIKAGLPEKVIKHWFRNTLFKERQRDKDSPYNFSVPPQMGIDLDTYEK
TGETKVVPLSNTEPKREPSPKPAETAAVQPLNLQAMMQQMQHANPFQFMDPSGMIPGP
MTQLSQTSSSGRRANRTRFTDYQLRTLQQFFDKQAYPKDDDLEVLSKKLQLSPRVIVV
WFQNARQKARKIYENQPNHENTDRFVRTPGCNFQCKRCNLVFQRYYELIQHQQKKCYK
DDCLAQANDNKIVEESLTDEEKAQLIQQTALASINDAKPADLAKLLGASKSSTEALLK
LCESANPSPSSFHKRCPFCGLLFRSKQSLQEHIPSRHPQQHSVTPLDVDLLPNAEDVP
VLIPPATPEQKDLAGVLDLSSASQERDTLSISPFLGQSDDDPSEYVEENPGTFVAGMS
QSRSPTSNKRYRTHLTPTQVHVMKSVFGDYKTPSMTECEALGREIGLHKRVVQVWFQN
ARAKERKTRTTVGAGEEETTRSASTSCSLCNVEYTGRVTMQDHLFSLQHLGLLKAQLK
TETGHADTPSSAFPSENGNERRPQSARPSKPSTTQPAQLANLNLNGFPFNFMNGMMSG
GLPLVYDPTMFGTPVSMLQIPDVVKLRIKSDITAGLSTSRFTQDGLSVDELRQKLDAE
DAQTLTQKEVEVGWACPSCTNVFQQEVLLKNHQRTVCQGTESVQLLTY"
assembly_gap 537197..537684
/estimated_length=488
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 540435..540934
/estimated_length=500
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 544612..544711
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 573574..573673
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene complement(574851..577121)
/locus_tag="ANCCEY_04498"
mRNA complement(join(574851..575157,575245..575330,
576087..576186,576259..576415,576538..576606,
577043..577121))
/locus_tag="ANCCEY_04498"
/product="zinc finger, C2H2 type"
CDS complement(join(574851..575157,575245..575330,
576087..576186,576259..576415,576538..576606,
577043..577121))
/locus_tag="ANCCEY_04498"
/note="KEGG: spu:589396 1.0e-05 similar to glutathione
reductase; K00383 glutathione reductase (NADPH)"
/codon_start=1
/product="zinc finger, C2H2 type"
/protein_id="EPB76440.1"
/translation="MSSDFGVHQLLMPFDGNQPGPSRMMVESSTNTPPTTDTSDLLRL
LSEGNRSDSPKAHLERLLNSAVDMHDGPSPMALDATTPFSLGMDLSMYGDLTGAGALR
NSSKTLKCPKCNWHYKYQETLEIHMKEKHSDGEVKCGYCAENRVHPKLARGESYSCGY
KPYRCELCKYSTTTKGNLSIHMQSDKHLHAVQELPSSIGKRLVVYSSNPMSVNDVKHS
PITASLSCPPARSPPQDGDRSLVCVVCGCFSTDDMEEMIAHADKVRY"
assembly_gap 588903..589383
/estimated_length=481
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 593622..593721
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 604755..605205
/estimated_length=451
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 615657..615978
/estimated_length=322
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 618727..619172
/estimated_length=446
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 628947..630038
/estimated_length=1092
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 633709..634432
/estimated_length=724
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 636632..637341
/estimated_length=710
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
assembly_gap 642742..643395
/estimated_length=654
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
gene 644684..651590
/locus_tag="ANCCEY_04499"
mRNA join(644684..644710,646696..646817,647969..648133,
648182..648275,648334..648492,651374..651430,
651519..651590)
/locus_tag="ANCCEY_04499"
/product="hypothetical protein"
CDS join(644684..644710,646696..646817,647969..648133,
648182..648275,648334..648492,651374..651430,
651519..651590)
/locus_tag="ANCCEY_04499"
/inference="protein motif:HMMPfam:IPR013099"
/codon_start=1
/product="hypothetical protein"
/protein_id="EPB76441.1"
/db_xref="InterPro:IPR013099"
/translation="MPEKVSEVEVVVASIPYIVICILIFLYVVFGAFTFSVIDDFMDE
DTFATNFVSITTIGFGDVIVSPQNNFDTAILTLYLVGGIVIMTTFINGVVDHVLWIHY
LGREPTNIRKNVVWFGGESMTVQELIVVAASSFEISPRQLRVTLRNLDQIIALALNDE
LRKKSKVFLHSDSIFTDGALLENEESKMKHRLRAASSLSRMSTPCHEKNIAALKLIYC
RTCAKPGNEVTDG"
assembly_gap 663246..663345
/estimated_length=unknown
/gap_type="within scaffold"
/linkage_evidence="paired-ends"
CONTIG join(ASSL01015573.1:1..4528,gap(426),ASSL01015574.1:1..4729,
gap(339),ASSL01015575.1:1..10164,gap(unk100),
ASSL01015576.1:1..6469,gap(unk100),ASSL01015577.1:1..11832,
gap(496),ASSL01015578.1:1..2050,gap(414),ASSL01015579.1:1..1046,
gap(unk100),ASSL01015580.1:1..6126,gap(473),ASSL01015581.1:1..2715,
gap(209),ASSL01015582.1:1..648,gap(1038),ASSL01015583.1:1..6245,
gap(192),ASSL01015584.1:1..1953,gap(723),ASSL01015585.1:1..1842,
gap(695),ASSL01015586.1:1..6173,gap(2393),ASSL01015587.1:1..6602,
gap(unk100),ASSL01015588.1:1..5439,gap(117),ASSL01015589.1:1..1183,
gap(946),ASSL01015590.1:1..2365,gap(981),ASSL01015591.1:1..1149,
gap(275),ASSL01015592.1:1..2715,gap(310),ASSL01015593.1:1..13026,
gap(unk100),ASSL01015594.1:1..5192,gap(unk100),
ASSL01015595.1:1..2429,gap(unk100),ASSL01015596.1:1..2041,gap(311),
ASSL01015597.1:1..5010,gap(unk100),ASSL01015598.1:1..1966,gap(280),
ASSL01015599.1:1..6620,gap(3307),ASSL01015600.1:1..15038,gap(178),
ASSL01015601.1:1..896,gap(1250),ASSL01015602.1:1..9073,gap(471),
ASSL01015603.1:1..2422,gap(1118),ASSL01015604.1:1..1268,gap(1968),
ASSL01015605.1:1..5017,gap(169),ASSL01015606.1:1..9885,gap(1460),
ASSL01015607.1:1..10252,gap(unk100),ASSL01015608.1:1..5864,
gap(764),ASSL01015609.1:1..12565,gap(607),ASSL01015610.1:1..8943,
gap(242),ASSL01015611.1:1..8181,gap(724),ASSL01015612.1:1..9378,
gap(191),ASSL01015613.1:1..3587,gap(unk100),ASSL01015614.1:1..4178,
gap(379),ASSL01015615.1:1..17139,gap(unk100),
ASSL01015616.1:1..8755,gap(242),ASSL01015617.1:1..6302,gap(249),
ASSL01015618.1:1..722,gap(479),ASSL01015619.1:1..9596,gap(unk100),
ASSL01015620.1:1..5582,gap(unk100),ASSL01015621.1:1..4910,
gap(unk100),ASSL01015622.1:1..5495,gap(348),ASSL01015623.1:1..6497,
gap(unk100),ASSL01015624.1:1..1247,gap(unk100),
ASSL01015625.1:1..6200,gap(2144),ASSL01015626.1:1..3406,gap(635),
ASSL01015627.1:1..9596,gap(109),ASSL01015628.1:1..8205,gap(182),
ASSL01015629.1:1..12394,gap(unk100),ASSL01015630.1:1..1099,
gap(unk100),ASSL01015631.1:1..16288,gap(351),
ASSL01015632.1:1..5294,gap(294),ASSL01015633.1:1..2722,gap(2113),
ASSL01015634.1:1..4052,gap(unk100),ASSL01015635.1:1..3876,gap(735),
ASSL01015636.1:1..3192,gap(632),ASSL01015637.1:1..647,gap(unk100),
ASSL01015638.1:1..776,gap(232),ASSL01015639.1:1..1238,gap(688),
ASSL01015640.1:1..4740,gap(574),ASSL01015641.1:1..3406,gap(217),
ASSL01015642.1:1..2858,gap(753),ASSL01015643.1:1..6455,gap(unk100),
ASSL01015644.1:1..2298,gap(545),ASSL01015645.1:1..21242,gap(571),
ASSL01015646.1:1..6190,gap(3565),ASSL01015647.1:1..3862,
gap(unk100),ASSL01015648.1:1..1967,gap(unk100),
ASSL01015649.1:1..6735,gap(344),ASSL01015650.1:1..3322,gap(493),
ASSL01015651.1:1..8763,gap(unk100),ASSL01015652.1:1..1815,
gap(unk100),ASSL01015653.1:1..3168,gap(unk100),
ASSL01015654.1:1..3285,gap(1022),ASSL01015655.1:1..6320,gap(293),
ASSL01015656.1:1..2293,gap(716),ASSL01015657.1:1..7028,gap(152),
ASSL01015658.1:1..6312,gap(462),ASSL01015659.1:1..15917,gap(488),
ASSL01015660.1:1..2750,gap(500),ASSL01015661.1:1..3677,gap(unk100),
ASSL01015662.1:1..28862,gap(unk100),ASSL01015663.1:1..15229,
gap(481),ASSL01015664.1:1..4238,gap(unk100),
ASSL01015665.1:1..11033,gap(451),ASSL01015666.1:1..10451,gap(322),
ASSL01015667.1:1..2748,gap(446),ASSL01015668.1:1..9774,gap(1092),
ASSL01015669.1:1..3670,gap(724),ASSL01015670.1:1..2199,gap(710),
ASSL01015671.1:1..5400,gap(654),ASSL01015672.1:1..19850,
gap(unk100),ASSL01015673.1:1..1487)
//