LOCUS BACRGRRNB 7430 bp DNA linear BCT 22-MAR-2001
DEFINITION Bacillus subtilis 16S ribosomal RNA, 23S ribosomal RNA, and 5S
ribosomal RNA genes, complete sequence; and tRNA-Val, tRNA-Thr,
tRNA-Lys, tRNA-Leu, tRNA-Gly, tRNA-Leu, tRNA-Arg, tRNA-Pro,
tRNA-Ala, tRNA-Met, tRNA-Ile, tRNA-Ser, tRNA-Met, tRNA-Asp,
tRNA-Phe, tRNA-His, tRNA-Gly, tRNA-Ile, tRNA-Asn, tRNA-Ser, and
tRNA-Glu genes, complete sequence.
ACCESSION K00637 M10606
VERSION K00637.1
KEYWORDS .
SOURCE Bacillus subtilis
ORGANISM Bacillus subtilis
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
REFERENCE 1 (bases 4897 to 7430)
AUTHORS Green,C.J. and Vold,B.S.
TITLE Sequence analysis of a cluster of twenty-one tRNA genes in Bacillus
subtilis
JOURNAL Nucleic Acids Res. 11 (16), 5763-5774 (1983)
PUBMED 6310512
REFERENCE 2 (bases 1 to 1168)
AUTHORS Stewart,G.C. and Bott,K.F.
TITLE DNA sequence of the tandem ribosomal RNA promoter for B. subtilis
operon rrnB
JOURNAL Nucleic Acids Res. 11 (18), 6289-6300 (1983)
PUBMED 6312417
REFERENCE 3 (bases 1 to 7430)
AUTHORS Green,C.J., Stewart,G.C., Hollis,M.A., Vold,B.S. and Bott,K.F.
TITLE Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon,
rrnB
JOURNAL Gene 37 (1-3), 261-266 (1985)
PUBMED 2414156
REFERENCE 4 (sites)
AUTHORS Su,S.L. and Dubnau,D.
TITLE Binding of Bacillus subtilis ermC' methyltransferase to 23S rRNA
JOURNAL Biochemistry 29 (25), 6033-6042 (1990)
PUBMED 2116903
COMMENT [3] revises [1],[2].
[4] sites for [1],[2] and [3].
Draft entry and sequence in computer readable form for [1],[2],[3]
kindly provided by K.F.Bott, 26-DEC-1985.
The RNAs, encoded by the sequence presented below, are probably
transcribed as one polycistronic unit, including the tRNA region,
because there are no obvious terminator stem loop structures until
after the end of the tRNA region at positions 7245-7272 and
7392-7413 [3].
[1] notes that though the Ile-tRNA-nau sequence has the methionine
anticodon 'cau', it is highly homologous to Ile-tRNA-gau; the 'c'
in the wobble position may be post-transcriptionally modified to
recognize 'aua' codons.
Promoter P1 is located at positions 184-189 (-35 region) and
207-213 (-10 region), and P2 at 276-281 (-35 region) and 299-304
(-10 region). A third promoter region could be at positions
5517-5522. A potential stem-loop structure, necessary for
processing of the mature 16S rRNA, is found at positions 327-360
[2].
FEATURES Location/Qualifiers
source 1..7430
/organism="Bacillus subtilis"
/mol_type="genomic DNA"
/strain="W168"
/sub_strain="PY79"
/db_xref="taxon:1423"
rRNA 485..2034
/product="16S ribosomal RNA"
old_sequence 504
/citation=[2]
old_sequence 571..573
/citation=[2]
rRNA 2203..5129
/product="23S ribosomal RNA"
old_sequence 5029..5031
/citation=[1]
rRNA 5185..5300
/product="5S ribosomal RNA"
tRNA 5322..5397
/product="tRNA-Val"
/note="codon recognized: GUA"
tRNA 5430..5504
/product="tRNA-Thr"
/note="codon recognized: ACA"
tRNA 5543..5618
/product="tRNA-Lys"
/note="codon recognized: AAA"
tRNA 5629..5715
/product="tRNA-Leu"
/note="codon recognized: CUG; Leu-tRNA-cug"
tRNA 5721..5795
/product="tRNA-Gly"
/note="codon recognized: GGC; Gly-tRNA-ggc"
tRNA 5810..5895
/product="tRNA-Leu"
/note="codon recognized: UUA; Leu-tRNA-uua"
tRNA 5905..5981
/product="tRNA-Arg"
/note="codon recognized: CGU"
tRNA 5997..6073
/product="tRNA-Pro"
/note="codon recognized: CCA"
tRNA 6079..6151
/product="tRNA-Ala"
/note="codon recognized: GCA"
old_sequence 6165..6167
/citation=[1]
tRNA 6172..6248
/product="tRNA-Met"
/note="codon recognized: AUG"
tRNA 6251..6327
/product="tRNA-Ile"
/note="Ile-tRNA-nau"
tRNA 6334..6425
/product="tRNA-Ser"
/note="codon recognized: UCA; Ser-tRNA-uca"
tRNA 6443..6519
/product="tRNA-Met"
/note="codon recognized: AUG"
tRNA 6531..6607
/product="tRNA-Asp"
/note="codon recognized: GAC"
tRNA 6620..6695
/product="tRNA-Phe"
/note="codon recognized: UUC"
tRNA 6712..6788
/product="tRNA-His"
/note="codon recognized: CAC"
tRNA 6799..6872
/product="tRNA-Gly"
/note="codon recognized: GGA; Gly-tRNA-gga"
tRNA 6888..6964
/product="tRNA-Ile"
/note="codon recognized: AUC; Ile-tRNA-gau"
tRNA 6975..7049
/product="tRNA-Asn"
/note="codon recognized: AAC"
tRNA 7053..7143
/product="tRNA-Ser"
/note="codon recognized: AGC; Ser-tRNA-agc"
tRNA 7169..7240
/product="tRNA-Glu"
/note="codon recognized: GAA"
BASE COUNT 1906 a 1694 c 2125 g 1705 t
ORIGIN
1 ctttaatgct ccccttgtgg tcatcagtat ttagttcgtt tcacatacaa gaaaacgaaa
61 aaaacaacaa gatcacatga ctgatgtata tgttctttta agaaacttat atgatacaca
121 cgctttagaa atcatggcga ggattatagt ttatttgttt tatagatttt ttttaaaaaa
181 ctattgcaat aaataaatac aggtgttata ttattaaacg tcgctgatgc acagcggaca
241 caactagatg cttcaaaaca acttgaaaaa agttgttgac aaaaaagaag ctgaatgtta
301 tattagtaaa gctgcttcat tgagaagtaa cgaaatgatc tttgaaaact aaacaagaca
361 aaacgtacct gttaattcag tttttaaaaa tcgcactgcg atgtgcgtat catcaaacag
421 ggcctgcacg acgcaggtca cacaggtgtc gccgcaggat gcggtgaact taacctgtga
481 tccatttatc ggagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca
541 tgcaagtcga gcggacaggt gggagcttgc tccgatgtta gcggcggacg ggtgagtaac
601 acgtgggtaa cctgcctgta agactgggat aactccggga aaccggggct aataccggat
661 ggttgtttga accgcatggt tcaaacataa aaggtggctt cggctaccac ttacagatgg
721 acccgcggcg cattagctag ttggtgaggt aacggctcac caaggcaacg atgcgtagcc
781 gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc ctacgggagg
841 cagcagtagg gaatcttccg caatggacga aagtctgacg gagcaacgcc gcgtgagtga
901 tgaaggtttt cggatcgtaa agctctgttg ttagggaaga acaagtaccg ttcgaacagg
961 gcggtacctt gacggtacct aaccagaaag ccacggctaa ctacgtgcca gcagccgcgg
1021 taatacgtag gtggcaagcg ttttccggaa ttattgggcg taaagggctc gcaggcggtt
1081 tcttaagtct gatgtgaaag cccccggctc aaccggggag ggtcattgga aactggggaa
1141 cttgagtgca gaagaggaga gtggaattcc acgttgtagc ggtgaaatgc gtagagatgt
1201 ggaggaacac cagtggcgaa ggcgactctc tggtctgtaa ctgacgctga ggagcgaaag
1261 cgtggggagc gaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa
1321 gtgttagggg gtttccgccc cttagtgctg cagctaacgc attgagcact ccgcctgggg
1381 agtacggtcg caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc
1441 atgtggttta attcgaagca acgcgaagaa ccttactagg tcttgacatc ctctgacaat
1501 cctagagata ggacgtcccc ttcggggcag agtgacaggt ggtgcatggt tgtcgtcagc
1561 tcgtgtcgtg agatgttggg ttaagtcccg caacgagcgc aacccttgat cttagttgcc
1621 agcattcagt tgggcactct aaggtgactg ccggtgacaa accggaggaa ggtggggatg
1681 acgtcaaatc atcatgcccc ttatgacttg ggctacacac gtgctacaat ggacagaaca
1741 aagggcagcg aaccgcgagg ttaagccaat cccacaaatc tgttctcagt tcggatcgca
1801 gtctgcaact cgactgcgtg aagctggaat cgctagtaat cgcggatcag catgccgcgg
1861 tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt tgtaacaccc
1921 gaagtcggtg aggtaacctt ttaggagcca gccgccgaag gtgggacaga tgattggggt
1981 gaagtcgtaa caaggtagcc gtatcggaag gtgcggctgg atcacctcct ttctaaggat
2041 attatacgga atataagacc caaggtctta taaacagaac gttccctgtc ttgtttagtt
2101 ttgaaggatc attccttcga aacgtgttct ttgaaaacta gataacagta gacatcacat
2161 tcaattagta acacaagata tcacatagtg attcttttta acggttaagt tagaaagggc
2221 gcacggtgga tgccttggca ctaggagccg atgaaggacg ggacgaacac cgatatgctt
2281 cggggagctg taagcaagct ttgatccgga gatttccgaa tggggaaacc caccactcgt
2341 aatggagtgg tatccatatc tgaattcata ggatatgaga aggcagaccc ggggaactga
2401 aacatctaag tacccggaga agagaaagca aatgcgattc cctgagtagc ggcgacgaac
2461 acgggatcag cccaaaccaa gaggcttgcc tctgtggttg taggacactc tgtacggagt
2521 tacaaaagaa cgaggtagat gaagaggtct ggaaagggcc cgccatagga ggtaacagcc
2581 ctgtagtcaa aacttcgttc tctcctgagt ggatcctgag tacggcggaa cacgtgaaat
2641 tccgtcggaa tccgggagga ccatctccca aggctaaata ctccctagtg accgatagtg
2701 aaccagtacc gtgagggaaa ggtgaaaagc accccggaag gggagtgaaa gagatcctga
2761 aaccgtgtgc ctacaagtag tcagagcccg ttaacggtga tggcgtgcct tttgtagaat
2821 gaaccggcga gttacgatcc cgtgcaaggt taagcagaag atgcggagcc gcagcgaaag
2881 cgagtctgaa tagggcgcat gagtacgtgg tcgtagaccc gaaaccaggt gatctaccca
2941 tgtccagggt gaagttcagg taacactgaa tggaggcccg aacccacgca cgttgaaaag
3001 tgcggggatg aggtgtgggt aggggtgaaa tgccaatcga acctggagat agctggttct
3061 ctccgaaata gctttagggc tagcctcaag gtaagagtct tggaggtaga gcactgattg
3121 gactaggggc cctcaccggg ttaccgaatt cagtcaaact ccgaatgcca atgacttatc
3181 cttgggagtc agactgcgag tgataagatc cgtagtcgaa agggaaacag cccagaccgc
3241 cagctaaggt cccaaagtat acgttaagtg gaaaaggatg tggagttgct tagacaacca
3301 ggatgttggc ttagaagcag ccaccattta aagagtgcgt aatagctcac tggtcgagtg
3361 actctgcgcc gaaaatgtac cggggctaaa cgtatcaccg aagctgcgga ctgttcttcg
3421 aacagtggta ggagagcgtt ctaagggctg tgaagccaga ccggaaggac tggtggacgg
3481 cttagaagtg agaatgccgg tatgagtagc gaaaagaggg gtgagaatcc ctccaccgaa
3541 tgcctaaggg ttcctgagga aggctcgtcc gctcagggtt agtcgggacc taagccgagg
3601 ccgaaaggcg taggcgatgg acaacaggtt gatattcctg taccacctcc tcaccatttg
3661 agcaatgggg ggtcgcagga ggatagggta agcgcggtat tggatatccg cgtccaagca
3721 gttaggctgg gaaataggca aatccgtttc ccataaggct gagctgtgat ggcgagcgaa
3781 atatagtagc gaagttcctg attccacact gccaagaaaa gcctctagcg aggtgagagg
3841 tgcccgtacc gcaaaccgtc acaggtaggc gaggagagaa tcctaaggtg atcgagagaa
3901 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctctgtta
3961 gggtgcaagc ccgagagagc cgcagtgaat aggcccaggc gactgtttag caaaaacaca
4021 ggtctctgcg aagccgtaag gcgaagtata ggggctgacg cctgcccggt gctggaaggt
4081 taagaggagc gcttagcgta agcgaaggtg cgaattgaag ccccagtaaa cggcggccgt
4141 aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa
4201 ggcgcaacga tctgggcgct gtctcaacga gagactcggt gaaattatag tacctgtgaa
4261 gatgcaggtt acccgcgaca ggacggaaag accccgtgga gctttactgc agcctgatat
4321 tgaatgttgg tacagcttgt acaggatagg taggagcctt ggaaaccgga gcgccagctt
4381 cggtggaggc atcggtggga tactaccctg gctgtattga ccttctaacc ccccgccctt
4441 atcgggcggg gagacagtgt caggtgggca gtttgactgg ggcggtcgcc tcctaaaagg
4501 taacggaggc gcccaaaggt tccctcagaa tggttggaaa tcattcgcag agtgtaaagg
4561 cacaagggag cttgactgcg agacctacaa gtcgagcagg gacgaaagtc gggcttagtg
4621 atccggtggt tccgcatgga agggccatcg ctcaacggat aaaagctacc ccggggataa
4681 caggcttatc tcccccaaga gctccacatc gacggggagg tttggcacct cgatgtcggc
4741 tcatcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc attaaagcgg
4801 tacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc gcgggcgctg
4861 gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacgcac cgctggtgta
4921 ccagttgttc tgccaagggc atcgctgggt agctatgtgc ggacgggata agtgctgaaa
4981 gcatctaagc atgaagcccc cctcaagatg agatttccca ttccgcaagg aagtaagatc
5041 cctgaaagat gatcaggttg ataggtctga ggtggaagtg tggcaacaca tggagctgac
5101 agatactaat cgatcgagga cttaaccata tttttgaatg atgtcacacc tgttatctag
5161 ttttgagaga acactctcaa tttgtttggt ggcgatagcg aagaggtcac acccgttccc
5221 ataccgaaca cggaagttaa gctcttcagc gccgatggta gtcgggggtt tccccctgtg
5281 agagtaggac gccgccaagc aattgcacgt tagtgcaata tggaggatta gctcagctgg
5341 gagagcatct gccttacaag cagagggtcg gcggttcgag cccgtcatcc tccaccattt
5401 ttcattatac atatcggttt tacatatatg ccggtgtagc tcaattggta gagcaactga
5461 cttgtaatca gtaggttggg ggttcaagtc ctcttgccgg caccactttt atatgatata
5521 atattcaagt ctattgtaag aagagccatt agctcagttg gtagagcatc tgacttttaa
5581 tcagagggtc gaaggttcga gtccttcatg gctcaccatt tacatgttgc ggatgtggcg
5641 gaattggcag acgcgctaga atcaggctct agtgtcttta cagacgtggg ggttcaagtc
5701 ccttcatccg caccatttct gcggaagtag ttcagtggta gaacaccacc ttgccaaggt
5761 gggggtcgcg ggttcgaatc ccgtcttccg ctccaactat accatccacg ccggggtggt
5821 ggaattggca gacacacagg acttaaaatc ctgcggtagg tgactaccgt gccggttcaa
5881 gtccggccct cggcattaag ttttgcgccc gtagctcaat tggatagagc gtttgactac
5941 ggatcaaaag gttaggggtt cgactcctct cgggcgcgcc atgatctata tgaaatcggg
6001 aagtagctca gcttggtaga gcacatggtt tgggaccatg gggtcgcagg ttcgaatcct
6061 gtcttcccga ccattcttgg ggccttagct cagctgggag agcgcctgct ttgcacgcag
6121 gaggtcagcg gttcgatccc gctaggctcc acttgatttc aaaaactatt tggcggtgta
6181 gctcagctgg ctagagcgta cggttcatac ccgtgaggtc gggggttcga tcccctccgc
6241 cgctaccaat ggacctttag ctcagttggt tagagcagac ggctcataac cgtccggtcg
6301 taggttcgag tcctacaagg tccaccacta tacggaggaa tacccaagtc tggctgaagg
6361 gatcggtctt gaaaaccgac agggtgtcaa agcccgcggg ggttcgaatc cctcttcctc
6421 cgccatacat attcctaatc atcgcggggt ggagcagttc ggtagctcgt cgggctcata
6481 acccgaaggt cgcaggttca aatcctgccc ccgcaaccaa attttaaaat ggtccggtag
6541 ttcagttggt tagaatgcct gcctgtcacg caggaggtcg cgggttcgag tcccgtccgg
6601 accgccattt aaatacttag gctcggtagc tcagttggta gagcaacgga ctgaaaatcc
6661 gtgtgtcggc ggttcgattc cgtcccgagc caccatttat caatatgctt tggcggttgt
6721 ggcgaagtgg ttaacgcacc agattgtggc tctggcattc gtgggttcga ttcccatcaa
6781 tcgccccaaa taaaaattgc gggtgtagtt tagtggtaaa acctcagcct tccaagctga
6841 tgtcgtgggt tcgattccca tcacccgctc catttctata tcgtcatggg cctgtagctc
6901 agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc attcaggccc
6961 accatgactt ttgttccaca gtagctcagt ggtagagcta tcggctgtta accgatcggt
7021 cgcaggttcg aatcctgcct gtggagccaa atggagaagt actcaagtgg ctgaagaggc
7081 gcccctgcta agggtgtagg tcgtgtaagc ggcgcgaggg ttcaaatccc tccttctccg
7141 ccatatgatt acagatatca taattatcgg cccgttggtc aagcggttaa gacaccgccc
7201 tttcacggcg gtaacacggg ttcgaatccc gtacgggtca tcccagaagc cttgcatatc
7261 ctgcaaggtt tttttgtttt tataaatcat gtatatgtct tagattttgt tctttatttt
7321 aaaaacagac tacaaaaatc tccatatatt tcgtttttct tcagaaaatg aagttaattg
7381 tctataagta taagccgttt cagggaaagg gctttttttt atttcttcga
//