LOCUS JO316724 799 bp mRNA linear TSA 23-FEB-2012 DEFINITION TSA: Nitella hyalina strain KGK0190 nhya_c58890_c mRNA sequence. ACCESSION JO316724 VERSION JO316724.1 DBLINK BioProject: PRJNA51163 KEYWORDS TSA; Transcriptome Shotgun Assembly. SOURCE Nitella hyalina ORGANISM Nitella hyalina Eukaryota; Viridiplantae; Streptophyta; Charophyceae; Charales; Characeae; Nitella. REFERENCE 1 (bases 1 to 799) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Broad phylogenomic sampling and the sister lineage of land plants JOURNAL PLoS ONE 7 (1), E29696 (2012) PUBMED 22253761 REFERENCE 2 (bases 1 to 799) AUTHORS Timme,R.E., Bachvaroff,T.R. and Delwiche,C.F. TITLE Direct Submission JOURNAL Submitted (12-JUL-2011) Cell Biology and Molecular Genetics, University of Maryland, Bioscience Research Building, College Park, MD 20947, USA COMMENT Two-step clustering process: First, trimmed 454 reads were clustered with MIRA v.2.9.43 using 454 EST assembly specific parameters. Second, the raw Sanger reads were combined with the 454 contigs + singleons (with quality scores) and were cleaned then clustered with CAP3 (100 bp plus 95 percent identity overlap). ##Assembly-Data-START## Assembly Method :: MIRA v. 2.9.43; CAP3 Sequencing Technology :: 454 GS FLX Titanium; Sanger ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..799 /organism="Nitella hyalina" /mol_type="mRNA" /strain="KGK0190" /db_xref="taxon:181804" BASE COUNT 187 a 194 c 208 g 210 t ORIGIN 1 ccggagctct cattgttttt gaagacaaca ttgatttctg ccgaacccgt ttccgaggga 61 acacggtcgt tacggcgaca gggagaaagg tcgttcaaca cgtctggatc ggtatgcaag 121 atcccgacga cggaaccgta accttctgcc caagacgccc gtcgaccggg atcgtcctcg 181 ctcatccttc gttcgcacca agtgtcatcg acacttgcag tcggtgccgg taggtgtgag 241 ccagcgagga aagaaatcca gctgcattca tgtccactca atttcgctgt attctaaacg 301 caatttcgta cgcgaatttg cggcgagtcg cgatgaaatc taggggacaa aggagtagta 361 tgttgttggt ttttcctttt caccattgtt gcttagaact taactcatca gttgttccta 421 ggaaggaagg aaggaaggaa ggtagagtgt cgtttctgcg gcaaccgtga tattaacaat 481 cagatggtaa gtggtgctat agatagggcg aggttccact tcgctgcatc cagcacttcc 541 ggccgtagat ccggctcatc aacggctgca actgctgcat acagcttagc ggaaccttgc 601 ccattggtag gtcgtgccac gtattagtac tccagattcc agaaggctta gcacgggcgc 661 atttatatcc cattgaaatt ttatcgcctt ttctaacttc ggggagctac gtatgtgagt 721 cattccgaca cgtcagatca gtcgtgtgac acgtcattac ttactgtgat gtgggatcgt 781 gagcgccgga atcctttga //